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The LightCycler® 480 Real-Time PCR SystemAccuracy. Versatility. Convenience.

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Go forward, confidently. Roche knows real-time PCR.

For more than 15 years, we’ve been innovating

instrumentation and reagents to bring the

best technologies to your lab. Our renowned

LightCycler® 480 System represents the

perfect combination of accuracy, versatility,

and convenience.

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Accuracy Count on reliable, reproducible results. Innovative design elements of the LightCycler® 480 System, such as the thermal block, optical system, and data analysis software, enable superior data accuracy.

VersatilityInstantly change between 96- and 384-well fast blocks without the need for tools or calibration. The LightCycler® 480 System is an open platform, which allows you to choose an automation partner that fits your workflow.

ConvenienceKeep your focus on what’s most important: your research.

• No required calibrations • No need for passive reference dyes • No need to change your light source

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The LightCycler® 480 thermal block unit includes a Therma-Base layer, a thin cavity lined with a wick structure and filled with fluid. Utilizing a series of condensation and evaporation events, the Therma-Base rapidly adjusts to temperature changes by efficiently dissipating heat across the block.

• Count on consistent temperature distribution across the plate.

• Obtain rapid, accurate heat equalization.

• Run fast PCR protocols. — 96 wells in < 1 hour — 384 wells in < 40 minutes

• Achieve reproducible data uniformity.

Innovative Thermal Block Fast, efficient temperature control for accurate results

Therma-Base technology improves data accuracy and consistency

Real-time PCR of a 442 bp amplicon on a 96-well LightCycler® 480 Instrument and Life Technologies 96-well Fast System. Ninety-six identical reactions were amplified on each instrument, with detection using a hydrolysis probe. Each instrument was programmed according to manufacturers’ specifications. (Roche R&D data)

Cq values from above plotted three-dimensionally based on the position in the actual 96-well PCR plate.

LightCycler® 480 System, 96 well Life Technologies 96-well Fast System

Cq Range: 25.74 – 26.1; 0.36 cycles Cq Range: 27.93 – 30.92; 2.99 cycles

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With an efficient, optimized design and a broad-spectrum, high-intensity LED lamp, the LightCycler® 480 optical system provides accurate data acquisition across the plate, eliminating the need for a passive reference dye.

• Ensure even excitation of all wells for the life of the instrument.

• Acquire raw data and avoid edge effect with the advanced mirror chamber.

• Capture all data simultaneously, without the need to scan, avoiding inconsistencies and mechanical instability.

• Experience the highest flexibility in choice of fluorescent dyes and detection formats, and enhanced multiplexing capabilities.

Advanced Optical System Industry-leading performance for accurate data capture

LightCycler® Cyan 500

LightCycler® Red 640 and 705

Cy5

LightCycler® Red 610 Fluorescein

and FAM

SYBR Green I and ResoLight Dye

HEX (VIC)

Fluorescent Dye Options Versatility to meet your application needs

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The innovative LightCycler® 480 software is characterized by advanced design and unique algorithms that enable rapid, accurate data analysis. Customizable views, a highly sophisticated sample editor, and convenient import and export functionalities are just a few of the benefits afforded by the software.

State-of-the-Art Analysis Software Fast, accurate data generation

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

LightCycler® 480 Software Applications

• Quickly start experiments from ready-to-use macros or templates for all applications.

• Apply basic or advanced analysis modes for both gene expression and genotyping studies.

• Define or edit sample information in plate or table views.

• Choose between research mode or a secured traceable database mode.

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Take your gene expression studies to the next level with fast and sophisticated software analysis options.

• Choose between basic and advanced modules for Cq calling and PCR efficiency correction.

• Use one or several targets and/or reference genes.

• Analyze targets and references between plates.

Want to learn more about our advanced data analysis options? Scan now.

Genotyping (SNP analysis) on the LightCycler® 480 System can be performed by endpoint or melting curve analysis. For both methods, software modules for fully automated analysis are included in the preinstalled LightCycler® 480 Software.

Gene Expression Analysis Absolute or relative quantification

Genotyping Advanced solutions for genetic variation research

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

The LightCycler® 480 HRM (Gene Scanning) module provides specificity and sensitivity with high sample throughput.

• Efficiently identify new variants via automated grouping algorithms.

• Reliably detect single-base variants – including class IV SNPs.

Want to know more about HRM? Scan now.

High Resolution Melting Rapid, efficient mutation discovery

AbsoluteQuantification

Gene ExpressionAnalysis

High ResolutionMelting

EndpointGenotyping

Melt CurveGenotyping

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Optimized Reagents to Simplify your Research Premium quality for sensitive, specific qPCR

Want to know more about our Innovative Probe Technology? Scan now.

Whether it’s high-performance master mix, custom qPCR panels, or ready-to-use reference genes, we have the reagents you need.

Master Mix Stable at Room Temperature • Storage of pre-pipetted plates in a place stacker • True walkaway automation

Customized qPCR Assays • Prevalidated and function tested • Single assays or custom panels

Innovative Probe Technology • 165 pre-tested, FAM-labeled

Universal ProbeLibrary probes • Ready-to-use reference genes

Selected Publications Hu S, Li G, Li H, Liu X, Niu J, Quan S, Wang F, Wen H, Xu Y, Li Q. Rapid detection of isoniazid resistance in Mycobacterium tuberculosis isolates by use of real-time-PCR-based melting curve analysis. J Clin Microbiol. 2014 May;52(5):1644-52. doi: 10.1128/JCM.03395-13. Epub 2014 Mar 5. PubMed PMID: 24599986; PubMed Central PMCID: PMC3993622.

Thomas V, Mazard B, Garcia C, Lacan P, Gagnieu MC, Joly P. UGT1A1 (TA)n genotyping in sickle-cell disease: high resolution melting (HRM) curve analysis or direct sequencing, what is the best way? Clin Chim Acta. 2013 Sep 23;424:258-60. doi: 10.1016/j.cca.2013.06.023. Epub 2013 Jul 1. PubMed PMID: 23827693.

Gocze K, Gombos K, Juhasz K, Kovacs K, Kajtar B, Benczik M, Gocze P, Patczai B, Arany I, Ember I. Unique microRNA expression profiles in cervical cancer. Anticancer Res. 2013 Jun;33(6):2561-7. PubMed PMID: 23749909.

Selected Videos Pacifici, M., Delbue, S., Kadri, F., Peruzzi, F. Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR. J. Vis. Exp. (83), e51172, doi:10.3791/51172 (2014).

Chugh, P., Tamburro, K., Dittmer, D. P. Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays. J. Vis. Exp. (46), e2210, doi:10.3791/2210 (2010).

Warth, S. C., Heissmeyer, V. Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation. J. Vis. Exp. (78), e50455, doi:10.3791/50455 (2013).

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As an open platform, the LightCycler® 480 System offers you the flexibility to utilize any pre-existing automation at your site or to choose an automation partner that best fits your needs. You won’t sacrifice on implementation time, as we’ve also partnered with select automation vendors to offer a plug-and-play solution for your needs.

• Installs on standard lab bench

• Any-orientation plate handling

• Built-in error detection and recovery

• One-touch teaching

• Features allow for CFR 21 Part 11 compliance

Integrated Robotics for True Walkaway Automation Versatility to meet your workflow needs

“We recently combined an automated pipetting system for setup of our real-time qPCR using the Roche LightCycler® System and have been very pleased with our results. This setup allows us to continuously run real-time reactions on the LightCycler® System throughout the night and is highly efficient in obtaining accurate and reproducible results in a very timely and streamlined manner. Our joint system adds speed, comfort and reliability to each step from PCR setup to analysis. This system allows us to run an average of ~4,000 reactions per day, which dramatically improves our productivity and offers us the ability to perform high-throughput projects with ease.”

Pauline Chugh, Ph.D., University of North Carolina, Dittmer Lab

“Integrating 20 separate instruments in a novel DNA-to-data automated laboratory system can be challenging. Keeping such a complicated system running around the clock, even more so. 1.8 million qPCR reactions and over 4,750 instrument hours later, I haven’t had a single production run failure from my four integrated LightCycler® 480 Instruments. Running over 30,000 qPCR reactions per day, the Roche LightCycler® 480 Instruments have increased throughput, reduced reagent cost, and improved both the reproducibility and accuracy of my assay.”

Kyle Lapham, University of California, San Francisco, Blackburn Lab

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Dimensions W 24 in x D 24 in x H 21.5 in (W 57.4 cm D 58.8 cm H 49.7 cm)

Weight 121 lb (55.6 kg)

Power consumption 200–240 Vac (50/60 Hz, 1500 VA)

Reaction volumes 5 µl–20 µl (384-well), 10 µl–100 µl (96-well)

Temperature control Peltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses)

Heating rate 96-well block: 4.4°C/s384-well block: 4.8°C/s

Cooling rate 96-well block: 2.2°C/s384-well block: 2.5°C/s

Excitation LightCycler® 480 LED Lamp (430 - 630 nm)

Detector Cooled monochrome CCD camera

Filters Excitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660

Computer Pentium PC with Windows 7

Preinstalled software

configuration

• Absolute Quantification • Relative Quantification Analysis• Tm Calling • Endpoint Genotyping• Absolute Quantification Analysis • Melting Curve Genotyping

Accessory software analysis module • Gene Scanning Software

Automation• Interface with LightCycler® 480 LIMS Interface Module • Barcode-assisted multiwell plate scanning • Plate loading capability

Data management 21 CFR Part 11 compatibility

LightCycler® 480 Instrument Characteristics

Overview of excitation and emission filters, dyes, and detection formats. The LightCycler® 480 Instrument employs a high-intensity LED that emits light over a broad wavelength range (430–630 nm).

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Ordering Information

LightCycler® 480 Instruments and Accessories Product Cat. No. Pack Size

LightCycler® 480 Instrument II, 96-well 05 015 278 001 1 Instrument 1)

LightCycler® 480 Instrument II, 384-well 05 015 243 001 1 Instrument 1)

LightCycler® 480 Block Kit 96 Silver 05 015 219 001 1 Kit 2)

LightCycler® 480 Block Kit 384 Silver 05 015 197 001 1 Kit 2)

LightCycler® 480 Bar-Code Scanner 04 710 606 001 1 Scanner

LightCycler® 480 Software, Version 1.5.1 04 994 884 001 1 Software Package

LightCycler® 480 LIMS Interface Module 05 066 310 001 1 Software Package

LightCycler® 480 Gene Scanning Software 05 103 908 001 1 Software Package

LightCycler® 480 Multiple Plate Analysis Software 05 075 122 001 1 Software Package

LightCycler® 480 Multiwell Plate 96, white 04 729 692 001 50 Plates / 50 Foils

LightCycler® 480 Multiwell Plate 384, white 04 729 749 001 50 Plates / 50 Foils

LightCycler® 480 Multiwell Plate 96, clear 05 102 413 001 50 Plates / 50 Foils

LightCycler® 480 Multiwell Plate 384, clear 05 102 430 001 50 Plates / 50 Foils

LightCycler® 480 Sealing Foil 04 729 757 001 50 Foils

1) Instrument package includes LightCycler® 480 Instrument, LightCycler® 480 thermal block unit (96- or 384-well), LightCycler® 480 Software, Version 1.5.1, LightCycler® 480 Instrument Operator’s Manual, LightCycler® 480 LED Lamp (spare lamp). A Pentium desktop PC is supplied with the instrument.

2) Kit package includes LightCycler® 480 thermal block unit (96- or 384-well), block cover, storage box.

LightCycler® 480 Reagents and Assays Product Cat. No. Pack Size

LightCycler® 480 SYBR Green I Master

(2x concentrated)04 707 516 00104 887 352 001

5 1 ml (500 20 µl reactions)10 5 ml (5000 20 µl reactions)

LightCycler® 480 Probes Master

(2x concentrated)

04 707 494 00104 887 301 00104 902 343 001

5 1 ml (500 20 µl reactions)10 5 ml (5000 20 µl reactions)1 50 ml (5000 20 µl reactions)

LightCycler® 480 Genotyping Master (5x concentrated) 04 707 524 001 4 384 µl (384 20 µl reactions)

LightCycler® 480 High Resolution Melting Master 04 909 631 001 5 1 ml (500 20 µl reactions)

LightCycler® 480 RNA Master Hydrolysis Probes 04 991 885 001 500 20 µl reactionsLightCycler® 480 Control Kit 04 710 924 001 1 Kit (3 control runs)

RealTime ready Cell Lysis Kit 06 366 821 001 1 kit (up to 50 40 µl reactions)

Transcriptor First Strand cDNA Synthesis Kit 3)

04 379 012 00104 896 866 001 04 897 030 001

1 Kit (50 reactions)1 Kit (100 reactions)1 Kit (200 reactions)

Universal ProbeLibrary Set, Human 04 683 633 001 1 Set 4)

Universal ProbeLibrary Set, Mouse 04 683 641 001 1 Set 4)

Universal ProbeLibrary Set, Rat 04 683 650 001 1 Set 4)

Universal ProbeLibrary Extension Set 04 869 877 001 1 Set 4)

Universal ProbeLibrary Set, Human Reference Gene Assays 05 046 114 001 1 Set 4)

Universal ProbeLibrary, Human PBGD Gene Assay 05 046 149 001 500 reactions

Universal ProbeLibrary, Human HPRT Gene Assay 05 046 157 001 500 reactions

Universal ProbeLibrary, Human ACTB Gene Assay 05 046 165 001 500 reactions

Universal ProbeLibrary, Human PGK1 Gene Assay 05 046 173 001 500 reactions

RealTime ready Focus Panels For detailed information please visit www.realtimeready.roche.com

RealTime ready DNA Probes Master (5x concentrated) 05 502 381 001 5 1 ml (1,250 x 20 µl reactions)

3) For detailed information, visit http://lifescience.roche.com/pcr4) For detailed information, visit www.universalprobelibrary.com

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Roche Diagnostics Corporation 9115 Hague Road Indianapolis, Indiana 46256

http://lifescience.roche.com

For life science research only. Not for use in diagnostic procedures.

For patent license limitations for individual products please refer to: www.technical-support.roche.com

LIGHTCYCLER, REALTIME READY, RESOLIGHT, and HYBPROBE are trademarks of Roche. Exiqon and ProbeLibrary are registered trademarks of Exiqon A/S, Vedbaek, Denmark. SYBR is a registered trademark of Life Technologies Corporation. All other product names and trademarks are the property of their respective owners.