Program for the 17th Meeting of the European Society for Pigment Cell Research

Geneva, Switzerland,

Tuesday 11th to Thursday 13th of September 2012.

Centre Médical Universitaire 1, Rue Michel-Servet

1211 Geneva 4

The Melanocyte and its Environment

Sponsors

-Krebsliga Schweiz, Ligue Suisse contre le Cancer, Lega Svizzera contro il Cancro -Krebsfoschung Schweiz, Recherche Suisse contre le Cancer, Ricerca Svizzera contro

il Cancro -Colorix SA -Zeiss AG -Bucher Biotec AG -Ligue Genevoise contre le Cancer -Leica Microsystems -University of Geneva, Switzerland -European Society for Pigment Cell Research Local organizing committee: Nicole Aebischer (Geneva) Friedrich Beermann (Lausanne) Lionel Fontao (Geneva) Marie-Claude Jacquier (Geneva) Severine Tabone-Eglinger (Geneva) Monique Wehrle-Haller (Geneva) International experts: Friedrich Beermann (CH) Dot Bennett (UK) Anja Bosserhoff (GER) Heather Etchevers (F) Lionel Larue (F) Marie-Dominique Galibert (F) Jose-Carlos Garcia-Borron (E) Robert Kelsh (UK) Luisa Lanfrancone (I) Lluis Montoliu (E) Alessandra Napolitano (I) Elisabeth Patton (UK) Mauro Picardo (I) Lukas Sommer (CH) Eirikur Steingrimsson (Iceland) Alain Taïeb (F) Bernhard Wehrle-Haller (CH) FAL = Fritz Anders Lecture, KL = keynote lecture, IS = invited speaker, OP = oral presentation

Program, sessions and speakers (Lecture Hall B400): 1st day (Tuesday, 11. September 2012)

8:00-9:30 ESPCR council meeting 9:30 Opening of Registration and Poster set up (foyer) 10:00-12:00 Special interest group meeting supported by ESPCR

-Development

12:30-13:00 Opening ceremony of the 17th ESPCR meeting: -Lionel Larue, President of the ESPCR -Bernhard Wehrle-Haller, Head of the local organizing committee

13:00 – 13:30 Fritz Anders Lecture: Chair: Lionel Larue FAL Friedrich Beermann (Lausanne, Switzerland)

Tyrosinase - transgenic mice and beyond ...

13:30 – 15:15 1st Session: Development of the Melanocyte: Chairs: Elizabeth Patton and Friedrich Beermann

IS1 Robert Kelsh (Bath, UK) Expanding our understanding of the zebrafish melanocyte gene regulatory network

IS2 Tosso Leeb (Bern, Switzerland) Hypopigmentation phenotypes in domestic animals

OP1 Mitch Levesque (Zürich, Switzerland) Tissue-specific transcriptomes and extracellular protein interactions identify gene

regulatory networks involved in neural crest development and zebrafish pigmentation OP2 Verena Kottler (Tübingen, Germany)

Kita and csf1ra mutants provide novel insights into pigment pattern formation in the guppy, Poecilia reticulata

OP3 Davide Seruggia (Madrid, Spain) The nuclear structure of the mouse tyrosinase locus

OP4 Edouard Reyes-Gomez et al (Maisons-Alfort, France) Sbno2 overexpression in Dct-expressing cells alters melanocyte and nerve

development in mice

15:15-15:45 Coffee break 15:45 – 16:45 2nd Session: The Bright and Dark side of the Sun

Chairs: Mauro Picardo and Alain Taïeb IS3 Alessandra Napolitano (Naples, Italy)

The bright and dark side of red hair pigments IS4 Marie-Dominique Galibert (Rennes, France)

How does USF1 orchestrate the UV-response?

16:45-18:00 Flash-Talks: Chair: Lluis Montoliu

OP5 Toyoko Akiyama (Nagoya, Japan) Effects of pigmentation related genes on the fibromelanosis phenotype in

Silky chickens OP6 Melanie Domingues (Paris, France)

Increased motility and invasiveness of melanoma cells overexpressing ICAT, an inhibitor of β-catenin

OP7 Sara Maia (Lisboa, Portugal) Optimization of a Retinal Pigment Epithelium differentiation protocol driven

by ectopic expression of eye transcription factors OP8 Lionel Larribère (Heidelberg, Germany)

Human iPS cell-based modeling of hyperpigmentation in Neurofibromatosis type 1 (NF1)

OP9 Chandana Basu Mallick (Tartu, Estonia) An insight on SLC24A5 gene and its role in human skin color variation

OP10 Sabine Druillennec (Paris, France) Raf signaling in melanocyte and melanoma development

OP11 Kate Brown (Norfolk, UK) Investigating heterogeneous cell populations in melanoma tumours

OP12 Phil Cheng (Zürich, Switzerland) DNA methylation in melanoma phenotype switching

OP13 Mohammad Krayem (Brussels, Belgium) Vemurafenib stimulates pigmentation in V600EBRAF melanoma cell lines through

MITF stimulation and cell differentiation

18:00-20:00 Poster Viewing and Welcome Reception (Foyer)

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2nd day (Wednesday, 12. September 2012)

9:00-10:30 3rd Session: Adhesion and Migration of Melanocyte and Keratinocytes Chairs: Lionel Fontao and Lukas Sommer

IS5 Laura Machesky (Glasgow, UK) Role of the Rac1 GTPase in melanoblast motility and melanoma invasion

and metastasis IS6 Arnoud Sonnenberg (Amsterdam, NL)

Regulation of hemidesmome dynamics during cell migration OP14 Lionel Larue (Paris, France)

Lack of PTEN overcomes senescence barrier and promotes melanoma metastasis on a NRAS background

10:30-11:00 Coffee break

11:00-12:30 4th Session: From Structure to Function and Therapeutics: Chairs: Robert Kelsh and Alessandra Napolitano

IS7 Eirikur Steingrimsson (Reykjavik, Iceland) DNA binding specificity and restricted dimerization properties of the melanocyte

master regulator MITF IS8 Olivier Michielin (Lausanne, Switzerland)

Rational design of new therapeutic strategies in stage IV melanoma OP15 Francoise Degoul (Clermont-Ferrand, France)

Targeted radionuclide therapy for pigmented melanomas OP16 Marie Dutreix (Paris, France)

New DNA repair inhibitors for Melanoma treatment: Dbait OP17 Christine Grill (Reykjavik, Iceland)

MITF and IRF4 regulate human pigmentation

12:30-14:00 Lunch buffet and poster viewing

14:00-14:45 Keynote lecture: Microenvironment and Cancer

Chair: Richard Marais

KL1 Doug Hanahan (Lausanne, Switzerland) Melanoma and its environment

14:45-16:30 5th Session: Stem Cells Chairs: Jo Lambert and Colin Goding IS9 Dot Bennett (London, UK)

Overview: stem cell concepts for melanocytes and melanoma IS10 Yann Barrandon (Lausanne, Switzerland)

Keratinocyte stem cells IS11 Colin Goding (Oxford, UK)

A key chromatin remodelling complex controls MITF expression and activity. OP18 Severine Tabone-Eglinger (Geneva, Switzerland)

Membrane-bound KitL: kinase dependent and independent functions through the c-kit receptor to control stem cells

16:30-17:00 Coffee break

17:00-19:00 6th Session: Hyperpigmentation:

Chairs: Heather Etchevers and Dot Bennett IS12 Jo Lambert (Gent, Belgium)

The melanocyte and its microenvironment : lessons from the clinic IS13 Alain Taïeb (Bordeaux, France)

TBA IS14 Jose Carlos Garcia-Borron (Murcia, Spain)

Relationships of melanocortin 1 receptor signaling to the cAMP and mitogen-activated protein kinase ERK1 and ERK2 pathways in heterologous and human melanoma cells

OP19 Andre Furger (London, UK) MC1R poly(A) site and its role to regulate intergenic splicing and the expression of

chimeric MC1R-Tubulin beta III receptors OP19bis Kasraee Behrooz (Geneva, Switzerland)

Evaluation of a novel colorimetric technology for discrimination and measurement of cutaneous pigmentation and erythema

19:30 Gala-dinner

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3rd day (Thursday, 13. September 2012)

08:30-10:30 7th Session: The Melanocyte Environment in Health and Disease: Chairs: Eugene Healy and Jose-Carlos Garcia-Borron

IS15 Eugene Healy (Southampton, UK) Regulating keratinocyte cancer; battles within battles

IS16 Mauro Picardo (Rome, Italy) Vitiligo, a multi-pathways process: a metabolic view

IS17 Salem Chouaib (Villejuif, France) TBA

OP20 Muriel Cario-André (Bordeaux, France) Fibroblasts regulate both physiological and pathological skin pigmentation in

vitro and in vivo OP21 Tag S. Anbar (Al-Minya, Egypt)

Inflammatory cells in vitiligo: is it a rare finding?

10:30-11:00 Coffee break

11:00-12:30 8th Session: Melanoma and the Role of the Microenvironment:

Chairs: Laura Machesky and Luisa Lanfrancone IS18 Elisabeth Patton (Edinburgh, UK)

A novel role for the phosphatase PRL-3 in melanocyte regeneration IS19 Anja Bosserhoff (Regensburg, Germany)

miRNAs in malignant melanoma development and progression IS20 Corine Bertolotto (Nice, France)

MITF controls the senescence and stem cell-like properties of melanocyte cells OP22 Ruth Halaban (New Haven, USA)

The Landscape of Mutations in Melanomas Revealed by Exome Sequencing

12:30-14:00 Lunch and poster viewing 13:00-14:00 General Assembly of the ESPCR

14:00-16:00 8th Session: Melanoma and the Role of the Microenvironment:

Chairs: Anja Bosserhoff and Eirikur Steingrimsson IS21 Luisa Lanfrancone (Milan, Italy)

Molecular determinants of cell invasion in melanoma: in vitro and in vivo studies IS22 Lukas Sommer (Zürich, Switzerland)

Common regulators of neural crest stem cells and melanoma formation IS23 Adam Hurlstone (Manchester, UK)

Novel determinants of melanoma progression from zebrafish

IS24 Donata Rimoldi (Lausanne, Switzerland) TBA

OP23 Nallet-Staub et al (Paris, France) Characterization of the Hippo Pathway in Melanoma: Focus on the effectors

YAP and TAZ

16:00-16:30 Coffee break 16:30-17:30 8th Session: Melanoma and the Role of the Microenvironment:

Chairs: Marie-Dominique Galibert and Alain Mauviel IS25 Jose Neptuno Rodriguez Lopez (Murcia, Spain)

Methotrexate promotes MITF-driven phenotype-switching in melanomas IS26 Manfred Schartl (Würzburg, Germany)

Use of the medaka fish model to identify conserved molecular pathways in melanoma

OP24 Catherine André (Rennes, France) Melanoma in predisposed dog breeds: spontaneously occuring models for genetics

and therapies of human melanoma

17:30-18:00 Keynote Lecture Chair: Bernhard Wehrle-Haller KL2 Richard Marais (Manchester, UK)

BRAF in melanoma

18:00-18:15 Concluding remarks and welcome to the ESPCR2013 (Bernhard Wehrle-Haller and Graça Raposo)

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Late speaker abstracts:

MITF controls the senescence and stem cell-like properties of melanocyte cells Bertolotto Corine INSERM U1065, 151, avenue St Antoine de Ginestière, BP3194, 06204 Nice, France, bertolot@unice.fr

The transcription factor MITF is the master gene of the melanocyte lineage. Many reports sustain a role for MITF in melanoma disease. In this regards, we recently identified a gain of function mutation of MITF that predisposes to melanoma (MITFE318K). We demonstrated in vitro that MITFE318K increases the tumorigenic properties of melanocyte cells. Conversely, we showed that the sustained inhibition of MITF triggers senescence of melanoma cells. These results indicate that in some circumstances MITF can overcome the program of senescence and can favor melanoma development. However, we and other also provide evidences that transient inhibition of MITF increases the tumorigenic potential of melanoma cells. Indeed, we showed that MITF inhibition by siRNA in human and mouse melanoma cells triggered an increase in the expression of the stem cell markers Oct4 and Nanog. Further, low-MITF expressing cells are more prone to form tumors in mice than high-MITF expressing cells. A slow-growing population with low-Mitf level and high tumorigenic potential also exists spontaneously in human melanoma samples. Ablation of this population dramatically decreases tumor formation. I will give an overview of the complex role of MITF that appears at the heart of programs controlling the senescence and stem cell-like properties of melanocyte cells.

Novel determinants of melanoma progression from zebrafish Adam Hurlstone University of Manchester, Manchester, UK

Frequent driver mutations detected in BRAF, NRAS, CKIT, GNAQ and ERBB4 in melanoma, malignant melanocyte neoplasms, present opportunities for personalized medicines in the form of drugs targeting these oncogene products or their downstream signalling components like MEK. While promising effects have recently been achieved in the clinic for advanced melanoma particularly for small molecule inhibitors of BRAF, relapse appears inevitable and spurs the search for novel targets and mechanistic insight into resistance mechanisms. We and others have shown that expressing BRAF and RAS driver mutations in zebrafish melanocytes also induces melanocyte neoplasia with histological and molecular hallmarks of human melanoma. These animal models of melanoma afford new opportunities to identify novel determinants of progression, which could potentially be therapeutic targets. We identify the small GTPase Rac as a determinant of progression. Transcriptome profiling is also providing a systems view of an expression profile associated with progression that again is likely to harbor novel therapeutic targets.

List of posters (presenter underlined): 1stSession–DevelopmentoftheMelanocyte 1. Tomoko Adachi1,3, Yusuke Nagao1,2, Vicky Chau3, Masahiko Hibi1,2, Robert N.

Kelsh3, Hisashi Hashimoto1,2 Sox5 regulates the development of neural crest-derived pigment cells in medaka and zebrafish

2. (OP5) Toyoko Akiyama1, Ai Shinomiya1, Keiji Kinoshita2, Makoto Mizutani2, Yoichi Matsuda2

Effects of pigmentation related genes on the fibromelanosis phenotype in Silky chickens

3. Johansen, P.1, Andersen, J.D.1, Delgado, M.C.1, Christoffersen, S.R.2, Harder, S.2, Paulsen, R.R.2, Børsting, C.1 and Morling, N.1

The genetics of human eye colour using a novel eye colour classification method

4. (OP2) Verena A. Kottler, Detlef Weigel, Christine Dreyer Kita and csf1ra mutants provide novel insights into pigment pattern formation in the guppy, Poecilia reticulata

5. (OP1) Mitch Levesque1,2, Johannes Junker1, Christian Soellner1, Georg Otto1, Karsten Borgwart1, Christianne Nüsslein-Volhard1 Tissue-specific transcriptomes and extracellular protein interactions identify gene regulatory networks involved in neural crest development and zebrafish pigmentation

6. (OP4) Edouard Reyes-Gomez, Nelly da Silva, Stéphanie Gadin-Czerw, Jean-Jacques Panthier, Geneviève Aubin-Houzelstein

Sbno2 overexpression in Dct-expressing cells alters melanocyte and nerve development in mice

7. (OP3) Davide Seruggia1, Lluís Montoliu1,2

The nuclear structure of the mouse tyrosinase locus

8. J. Djian-Zaouche1, 2, C. Campagne1, 2, G. Egidy1, 2, 3, S. Jule1, 2, E. Reyes-Gomez1,

2, S. Gadin-Czerw1, 2, F. Bernex1, 2, A. Louise3, F. Relaix3, M. Buckingham3, F. Beermann4, J.J. Panthier3 and G. Aubin-Houzelstein1, 2, 3

Pax3GFP: a new reporter for the melanocyte lineage and melanoma cells in the mouse

9. F. Belaubre, S. Poigny, E. Questel, S. Julie, N. Castex-Rizzi, S. Bessau-Touya A Unique Three-Compound Association to improve Melasma and Solar Lentigo Hyperpigmentation

10. Jens Hoffmann, Daniel Fuchs, Tanja Klein, Oliver Engelking & Horst W. Fuchs Characterisation of a new melanocyte containing reconstructed epidermis

2ndSession–TheBrightandtheDarkSideoftheSun

11. Seunghyun Bang, Myoung Shin Kim, Sora Choi, Jeong Hwan Kim1, Chong Hyun Won, Mi Woo Lee, Jee Ho Choi, Kee Chan Moon, Sungeun Chang Inhibition of laser-induced post-inflammatory hyperpigmentation by tranexamic acid: in vitro study

12. Seunghyun Bang, Woo Jin Youn, Sora Choi, Kyunghyun Min, Chong Hyun Won, Mi Woo Lee, Jee Ho Choi, Kee Chan Moon, Sungeun Chang Human epidermal melanocytes express EGF receptor and respond to EGF via Erk signaling

13. Marta Abrisqueta1, Concepción Olivares Sánchez1, Ana B Pérez-Oliva1, Cecilia Herraiz1, José Carlos García-Borrón1, 2 and Celia Jiménez-Cervantes1, 2. Mahogunin Ring Finger 1 may contribute to MC1R-dependent ubiquitylation of β-arrestins

3rdSession–AdhesionandMigrationofMelanocyteandKeratinocyte

14. (OP14) A. Conde Perez1 , C. Longvert1, G.Gros1 , I. Puig1 , F. Beermann2 , Leon Van Kempen3 , L. Larue1

Lack of PTEN overcomes senescence barrier and promotes melanoma metastasis on a NRAS background

15. (OP6) M. Domingues, F. Rambow, L. Larue and J. Bonaventure Increased motility and invasiveness of melanoma cells overexpressing ICAT, an inhibitor of β-catenin

16. Ino F.1, Kaya G.2, Piguet V.3, Fontao L.1 Co-expression of Vimentin, Nestin and Plectin in melanomas

17. Valérie Petit*1, Fabrice Cordelières*2, Stuart Gallagher1, Olivier Debeir3 and Lionel Larue1

Automated cell tracking and analysis in phase contrast videos: Development of a java software based on combined mean-shift processes

4thSession–FromStructuretoFunctionandTherapeutics

18. (OP15) Viallard C1, Bonnet M1, Besse S1, Cardamone, E1, Mishellany F2, Cayre A2, Labarre P1, Miot-Noirault E1, Chezal J-M1, Sun Y-S3, Dutreix M4, Degoul F1 Targeted radionuclide therapy for pigmented melanomas

19. (OP16) Julian Biau1,2, Flavien Devun3, Amélie Crozet1,3, Cécile Laurent1, Mano Sayarath 3, Jian-sheng Sun3, Simon Saule1, and Marie Dutreix1

New DNA repair inhibitors for Melanoma treatment: Dbait

20. Christine Grill1, Kristín Bergsteinsdottir1, Margret H. Ögmundsdottir1, Veronique Pingault2 and Eirikur Steingrimsson1 Functional analysis of MITF mutations associated with Melanoma and with Waardenburg and Tietz syndromes

21. Maria S. Correia1,2, Giulia Bolasco3,4, Alistair N. Hume3,5, Abul K. Tarafder1, Francisco Pereira1, Clare E. Futter7, Duarte C. Barral1 and Miguel C. Seabra 1,2,3 Molecular characterization of melanin uptake and processing by keratinocytes

22. Anca Filimon, Sabina Zurac 1, Gabriela Negroiu The study of TRP-2 antigen expression in cell populations of melanocytic lesions revealed molecular events with possible implications in melanoma diagnosis and prognosis

23. (OP17) Christian Praetorius1, Christine Grill1, Alex Metcalf2, Alexander Schepsky1, Aaron Smith2, Kathleen Robinson3, Eric van Otterloo4, David Fisher3, Robert Cornell4, Rick Sturm2, and Eiríkur Steingrímsson1

MITF and IRF4 regulate human pigmentation

24. Hiroko Isoda1,2, Myra O. Villareal2, Junkyu Han1,2, Hideyuki Shigemori1 and Abdelfatteh EL Omri1

Hair growth promotion and melanogenesis regulatory effects of Erica multiflora extract

25. (OP7) Cristiana F. Pires1,2, Inês P. Rodrigues1, Sara Maia1, Martim D. Portal1, José S. Ramalho1, Miguel C. Seabra1,3 Optimization of a Retinal Pigment Epithelium differentiation protocol driven by ectopic expression of eye transcription factors

26. Marieke Raaijmakers1, Daniel Widmer1, Tania Herrera de Rolo1, Reinhard Dummer1, Mitch Levesque1

Exploring phenotypic heterogeneity in melanoma cultures

27. Jung-Min Shin1, Mi-Yoon Kim1, Hye in Choi1, Kyung-Cheol Sohn1, Chang Deok Kim1, Jeung-Hoon Lee1, Tae-Jin Yoon2, Young Lee1 Effect of Nrf2/Keap1 pathway on melanogenesis

28. L. Ondrušová, J. Réda, J. Vachtenheim, Z. Tuháčková Involvement of c-Src activity in the mTOR-dependent oncogenicity of melanoma cells

29. Myra O. Villareal1, Junkyu Han1,2, Kenjiro Ikuta3, Hideyuki Shigemori2, and Hiroko Isoda1,2 The role of MAPK and cAMP pathways in the melanogenesis and cell differentiation effects of hirsein A in B16 murine melanoma cells

5thSession–StemCells

30. (OP18) Séverine Tabone-Eglinger, Nicole Aebischer, Monique Wehrle-Haller, Marie-Claude Jacquier, David Boettiger and Bernhard Wehrle-Haller Membrane-bound KitL: kinase dependent and independent functions through the c-kit receptor to control stem cells

31. (OP8) L. Larribère, J. Utikal Human iPS cell-based modeling of hyperpigmentation in Neurofibromatosis type 1 (NF1)

32. Daniel Zingg1, Olga Shakhova1, Lynda Chin2, Friedrich Beermann3, Haruhiko Koseki4, Lukas Sommer1 Role of the epigenetic modifier EZH2 in melanoma

6thSession–Hyperpigmentation

33. (OP19) Martin Dalziel, Marina Kolesnichenko, Nuno Nunes, Ricardo Pires das Neves, Francisco Iborra, Colin Goding and André Furger MC1R poly(A) site and its role to regulate intergenic splicing and the expression of chimeric MC1R-Tubulin beta III receptors

34. Hsu C.1, Ahmadi S.2, Pourahmad M.3, Ali Mahdi H.4 Cysteamine cream as a new skin depigmenting product

35. Ahmadi S.1, Pourahmadi M.1, Aslani F.S.2, Kasraee B.3 Topical isonicotinic acid hydrazide (isoniazid) as a new skin depigmenting compound

36. (OP19bis) Baquié Mathurin1, Kasraee Behrooz 2

Evaluation of a novel colorimetric technology for discrimination and measurement of cutaneous pigmentation and erythema

37. (OP9) Chandana Basu Mallick1,2

An insight on SLC24A5 gene and its role in human skin color variation

38. Jin Hee Kim* Down-regulation of melanin synthesis by ori2 and its derivatives in Melan-a cells

7thSession–Themelanocyteenvironmentinhealthanddisease

39. (OP21) Tag S. Anbar,1; Ibrahim El-Ghareeb,2; Magda I. Assaf,3; Hamza Abdel-Raouf,; Sherif S. Awad,; Amal T. Abdel-Rahman1; Maha H. Ragaie1; Waleed M. Albalat2 Inflammatory cells in vitiligo: is it a rare finding?

40. Arunachalam M, Colucci R, Conti R, Dragoni F, Galeone M, Moretti S. Vitiligo and Psoriasis Comorbidity - a case control study in Italian patients

41. Barbara Bellei, Angela Pitisci and Mauro Picardo Inhibition of Melanogenesis by the Pyridinyl Imidazole Class of Compounds: Possible Involvement of the Wnt/β-catenin Signaling Pathway

42. (OP20) Cario-Andre Muriel1,2,3, Khaled Ezzedine1,2,3, Pain Catherine1,2, Guyonnet-Dupérat Véronique4, Bibeyran Alice4, Taieb Alain1,2,3 Fibroblasts regulate both physiological and pathological skin pigmentation in vitro and in vivo

43. Catherine Pain1,2,3, Laila Benzekri4, Yvon Gauthier, Khaled Ezzedine1,2,3, Alice Bibeyran5, Véronique Guyonnet-Dupérat5, Isabelle Lamrissi-Gracia 1,2, ,Alain Taieb1,2,3 and Muriel Cario-André1,2,3 Study of CCN3 (Nov) expression in normal skin and vitiligo skin

44. Maria Lucia Dell’Anna, Daniela Kovacs, Giorgia Cardinali Monica Ottaviani, Mauro Picardo Vitiligo pathogenesis: are mitochondria relevant players?

45. Duval C, Cohen-Dellarre C, Chagnoleau C, Bernerd F Demonstration of the role of dermal fibroblasts in regulating pigmentation by means of a functional full thickness pigmented skin model

46. Jennifer Allouche1, Lionel Larribère2, Gilles Lemaitre1, Xavier Nissan1, Marc Peschanski1, Christine Baldeschi1

Modeling pigmentary defects of neurofibromatosis type I using embryonic stem cells

47. Christian Muteba Baseke Epidemiology study of vitiligo in three private hospitals in Kinshasa About 25 Cases

48. Hye-In Choi1, Mi Yoon Kim2, Jung-Min Shin2, Gue-In Choi1, Hyungwon Kim1, Kyung-Cheol Sohn2, Dae-Kyoung Choi2, Chang Deok Kim2, Jeung-Hoon Lee2, Young Ho Lee1 The role of melanosome in human keratinocytes

49. Colucci R. *, Benvenga S. §, Arunachalam M.*, Dragoni F. *, Galeone M*. Lotti F.δ, Lotti T.#, Moretti S*. Increased thyroid hormone autoantibodies in a group of non-segmental vitiligo patients- Preliminary results

50. Laurence Denat, Laurent Marrot, Christophe Jones, Jean-Roch Meunier Characterization at the transcription level of metabolic equipment of normal human melanocytes and keratinocytes in culture

51. Duval C, Cohen-Dellarre C, Chagnoleau C, Bernerd F Demonstration of the role of dermal fibroblasts in regulating pigmentation by means of a functional full thickness pigmented skin model

52. Agustín España1, Celia Jiménez-Cervantes1, 2, André Furger3, José C García-Borrón1, 2, Cecilia Herraiz1. Functional characterization of MC1R-TUBB3 intergenic splice variants

53. Mi Yoon Kim1, Jung-Min Shin1, Hye-In Choi2, Dae-Kyoung Choi1, Kyung-Cheol Sohn1, Jeung-Hoon Lee1, Tae-Jin Yoon3 and Chang Deok Kim1 Effect of etopic expression of tyrosinase on wound healing

54. Daniela Kovacs 1, Hamza Abdel-Raouf 2, Mohamed Al-Khayyat 2, Eman Abdel-Azeem 2, Pietro Donati 1, Mauro Picardo 1, Tag S. Anbar 2. Melanocyte characterization in vitiligo punch grafting

55. JY Kim, JY Shin, MR Kim, SK Hann, SH Oh siRNA-mediated knockdown of COX-2 in melanocytes suppresses melanogenesis

56. Chieh-Shan Wu1,2,3, Mayumi Komine3,4, Seiki Fujimoto5, Hanako Ohmatsu3, Kanako Kikuchi3, Hisao Enami5, Yayoi Tada3, Hsin-Su Yu2, Mamitaro Ohtsuki4, Kunihiko Tamaki3 Narrow-band UVB induced skin pigmentation is correlated with epidermal keratinocyte proliferation: induction of keratinocyte proliferation as a novel strategy for the treatment of vitiligo

8thSession–Melanomaanditsenvironment

57. (OP24) Edouard Cadieu1,2*, Marc Gillard1,2*, Clotilde De Brito1,2, Jérome Abadie3, Béatrice Vergier4, Anne Sophie Guillory1,2, Patrick Devauchelle5, Fréderique Degorce6, Laëtitia Lagoutte1,2, Benoit Hédan1,2, Marie-Dominique Galibert1,2 and Catherine André1,2

Melanoma in predisposed dog breeds: spontaneously occuring models for genetics and therapies of human melanoma

58. (OP10) S. Druillennec1, C. Dorard1, A. Valluet1, C. Barbotin1, M. Larcher1, F. Beermann2, M. Baccarini3, L. Larue1, A. Eychène1. Raf signaling in melanocyte and melanoma development

59. (OP22) Michael Krauthammer1, Yong Kong2,3, Byung Hak Ha4, Perry Evans1, Antonella Bacchiocchi5, James P McCusker1, Elaine Cheng5, Matthew J Davis4, Gerald Goh6, 7, Murim Choi6, 7, Stephan Ariyan8, Deepak Narayan8, Ken Dutton-Regester 9,10, Ana Capatana1, Edna C Holman5, Marcus Bosenberg5, Mario Sznol11, Harriet M Kluger11,

Douglas E Brash5,6,12, David F Stern1, Miguel A Materin13, Roger S Lo14, Shrikant Mane6,15, Shuangge Ma16, Kenneth K Kidd6, Nicholas K. Hayward10, Richard P Lifton6,7, Joseph Schlessinger4, Titus J Boggon4, Ruth Halaban5* The Landscape of Mutations in Melanomas Revealed by Exome Sequencing

60. Fabrice Journe 1,#, Petra El Hajj 1,#, Murielle Wiedig 1, Ioanna Laios 2, François Salès 1, Marie-Dominique Galibert 3, Léon C van Kempen 4, Bassam Badran 5, Denis Larsimont 2, Ahmad Awada 6, Ghanem Ghanem 1 Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts disease-free and overall survival in high risk melanoma patients

61. (OP23) Flore Nallet-Staub, Cristèle Gilbert, Sophie Dodier, Véronique Bataille, Marius Sudol, Ling Li, Meenhard Herlyn, and Alain Mauviel Characterization of the Hippo Pathway in Melanoma: Focus on the effectors YAP and TAZ

62. Murielle Wiedig 1,#, Mimoune Berehab 1,#, Batiste Leroy 2, Ruddy Wattiez 2, Renato Morandini 1, Ahmad Awada 3, Ghanem Ghanem 1, Fabrice Journe 1 Proteomic analysis of V600EBRAF versus WTBRAF melanoma cell lines

63. (OP11) K. Brown, V. Sherwood. Investigating heterogeneous cell populations in melanoma tumours

64. Golovko, Anna1, Susanne Brükner1, Jörn Völker1, Veronica Höiom2, Johan Hansson2, Andersson, Leif1,3 Exon resequencing of PMEL17 in swedish melanoma patients

65. Delphine Javelaud, Dominique Dewolf, Elena Grigorenko and Alain Mauviel Simultaneous TaqMan® Q-PCR quantitation of hundreds of cancer-related genes in melanoma cell lines using the OpenArray® technology

66. Delphine Javelaud, Dominique Dewolf, Elena Grigorenko and Alain Mauviel Melanoma cell miRnome analysis using the TaqMan® OpenArray® technology: Modulation by TGF-β

67. Marie-Jeanne Pierrat, Véronique Marsaud, Alain Mauviel, and Delphine Javelaud Molecular Mechanisms underlying Inhibition of Microphthalmia-associated Transcription Factor (MITF) Expression by Transcription Factor GLI2 and Transforming Growth Factor-β

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