ORDERING INFORMATION

MicropipetteCapacity Color Stock No.

5 ul Orange MP-510 ul Violet MP-1020 ul Copper MP-2025 ul Brown MP-2530 ul Black MP-3050 ul Blue MP-50

100 ul Red MP-100200 ul Green MP-200250 ul Gold MP-250300 ul Lime MP-300400 ul Black MAP-400500 ul Blue MAP-500750 ul Green MAP-750

1000 ul Red MAP-1000

Tips, Disposable, Polypropylene

Stock No. MPT -2Recommended for use with 1 JLI through approx.150 JLI pipettes. Maximum dispensing volume up toapprox. 200 JLI for special applications. Overalllength-approx. 23/4".

Stock No. MPT -4

Recommended for pipettes dispensing 200 JLI

through 1 ,000 JLI. Overalllength-approx. 3".

WORK STATION, PIPET (pIs)W 0126 Recommended for use with all MP and MAP pipets

REPLACEMENT SPRINGS FORSIGMA FIXEO VOLUME PIPETS (pIs)

S 5010 For MP-5 through MP-1 00 PipetsS 4260 For MP-2OO through MP-300 PipetsS 1890 For MP-500 through MP-1000 PipetsS 6511 For MP-11 00 through MP-5000 Pipets

Note: The printing on a few of ourpipettes has tended to wear off pre-maturely. Please let us know if thisoccurs.

Sigma warrants that its products conform to the informationcontained in this and other Sigma publications. Purchasermust determine the suitability of the product for its pa~icularuse. See reverse side of invoice or packing slip for additionalterms and conditions of sale.

Thank you for your cooperation.

Please read instructions for bestresults with your precision-madeSIGMA Micropipette.CHEMICAL COMPANY

P.o. BOX 14508, ST. LOUIS, MO 63178Telephone 314-771-5750 1310/30 9014

PRECISION PIPETTING INSTRUCTIONS

--?~K 1.

Hold the SIGMA Micro-pipette perpendicular to thework station. Thrust thenozzle firmly into a clean tipand lift out the instrumentwith tip affixed. (Fig. 1).

4.

Immerse the tip approxi-mately 1/8" into the samplesolution. (Fig. 4).

~ Fig. 4---~"-'"'

---

Fig. 1

~~

/~,

-/

5.Allow the plunger to returnslowly to the release

position. (Fig. 5).

2.

Holding the Micropipette in

hand, give a slight twist to

the tip to insure a tight seal.

(Fig.2).

( .~

~

WJ Fig. 5

Fig. 2

-1- 6.Remove the tip from thesample solution. Withouttouching the tip opening,wipe off any excess samplewith a lint-free wiper .

3.

Depress the plunger com-

pletely before entry into

sample solution. (Fig. 3).-

See NOTES.

~ Fig. 3

Notes7.To complete empty the con-tents of the tip, hold the pointagainst the wall of the receivingvessel (Fig. 6) and depress theplunger slowly to the stop.(Fig. 7). The capillary actionbetween the wall of the receiv-ing vessel and the tip is animportant consideration inassuring that all of the samplehas been dispensed from the

tip.

...

4

~

Occasionally, it is possible that thecollar and nozzle of the Micropipettehave become loosened in transport.Before using, hand tighten thecollar firmly onto the barrel. Thencheck the nozzle and also firmlyhand tighten onto barrel.

~ At the start of each day's operation,depress and release plunger a fewtimes. Because of the strongpositive action of the Micropipette,it is necessary to depress andrelease the plunger smoothly andslowly.

Fig. 6 Fig.7

8a.After all of the contents havebeen dispensed, move the tipaway from the wall of the receiv-ing vessel before allowing theplunger to return to its release

position. (Fig. 8).

1After prolonged use, the disposabletips may leave a thin film of plasticon the nozzle. This may cause theSIGMA precision disposable tipsnot to seat properly. This film maybe removed by cleaning off the endof the nozzle with water and a

detergent.

ab.There are applications where itis necessary to dispense thecontents of the tip into a solu-tion already present in thereceiving vessel, without hold-ing the point against the wall.After dispensing all the con-tents and before releasing theplunger, touch the tip pointagainst the wall to remove anysample residue. Then, movepoint away from wall and re-lease the plunger. (Figs. 7 & 8).

1-.:1

Fig. 8

The SIGMA Micropipette has beendesigned to operate with SIGMAprecision disposable tips. Othervarieties of tips may appear to fitthe Micropipette, but performanceaccuracy (:t 1%) can be guaranteedonly when Sigma disposable tipsare used.

9.

Remove the used tip.

Guarantee REPRODUCTION OF ACCURACY PERSINGLE UNIT MICROPIPETTE.

PROCEDURE: each Micropipette received 20consecutive tests. A new tip was used for eachtest.

The SIGMA Micropipette is uncondi-tionally guaranteed for one year. If,for any reason, other than misuse,the Micropipette fails, return theinstrument to SIGMA and it will berepaired or replaced at no charge.

CoeffI-cIent

ofVariation

0.40;00.3%0.30;00.2%0.50;00.2%0.20;00.1%0.2%0.30;00.60;00.3%0.10;00.30;0

MeanValue

5.005 ul10.05 ul20.00 ul25.00 ul29.93 ul50.11 ul

100.59 ul200.31 ul251.05 ul301.19 ul399.54 ul500.55 uJ750.65 ul

1006.30 ul

StandardDeviation

0.022 ul0.03 ul0.06 ul0.07 ul0.15 ul0.11 ul0.22 ul0.38 ul0.53 ul0.99 ul2.20 ul1.53 ul1.22 ul3.27 ul

Size

5 ul10 ul20 ul25 ul30 ul50 ul

100 ul200 ul250 ul300 ul400 ul500 ul750 ul

1000 ul

Performance

The following statistical data wereobtained utilizing gravimetric mea-surements. An analytical balance,sensitive to 0.01 milligrams, wasused.

Since the Micropipette is designedto deliver micro-quantities, theoperating temperature is an impor-tant consideration. Each SIGMAMicropipette is rated and calibratedto deliver an intended volume withan accuracy of::!: 1% at 20-22°C.

PERFORMANCE PER BA TCH OFMICROPIPETTES.

PROCEDURE: In each test, 20 differentMicropipettes were used. A new tip was usedfor each test.

Coeffi-cient

ofVariation

0.4%

0.4%

0.5%

0.4%

0.5%

0.5%

0.5%

0.4%

0.3%

0.4%

0.7%

0.4%

0.1%

0.3%

MeanValue

5.005 ul10.00 ul20.00ul25.05 ul29.96 ul50.10 ul99.88 ul

200.77 ul251.22 ul301.67 ul399.58 ul500.66 ul750.36 ul

1003.63 ul

StandardDeviation

0.025 ul

0.04 ul

0.11 ul

0.12 ul

0.17 ul

0.28 ul

0.50 ul

0.83 ul

0.97 ul

1.42 ul

2.71 ul

2.09 ul

1.29 ul

3.81 ul

Size

5ul10 ul20 ul25ul30 ul50 ul

100 ul200 ul250 ul300ul400 ul500 ut750ul

1000 ul

Prior to making the tests, distilledwater, Micropipette, and disposabletips "Vere brought to 20-22°C.

Coeffi-cient

ofAccuracy

0.1%0.5%0.0%0.00;00.2%0.2%0.5%0.1%0.40;00.30;00.10;00.10;00.0%0.60;0

Coeffi-cient

ofAccuracy

0.1%0.0%0.0%0.2%0.1%o.~/o0.1%0.3%0.40;00.50;00.10;00.10;00.00;00.3%