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TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References...

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Columnchromatography celumn TLC Is up down demented I fn zstpi.mg IIHF Ital 4 nonpolar out 1st Model for Column Tweed chromatography most polar last Seperationtooly L S Prep TLC iii in
Transcript
Page 1: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

Columnchromatographycelumn TLC

Isup

downdemented

I

fn

zstpi.mg

IIHF Ital4 nonpolar out1st

Model forColumnTweed chromatography

mostpolar last

Seperationtooly LSPrep TLC

iiiin

Page 2: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

aa a t.TTC C C

cawwww

Im Gage 14 o8gopen Gage

Et Et Et EtH H H H

Fl packedCotton makeslurrySand ofSolidsupport 11 Column

Addsolvent witffffkeneftheats Adddislodge air bubbles up boils slurrypurge air Let it cool

gsofff.IO M mixing bubble19

25mL500mL solyennt 50mLwork

Typumb

Gage

1 so coolesttubesI

F

II eEEi I

Page 3: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

Loading Column Comparison to TLC

a a

Ee.io fgl jjfresoumEE'sED's

O_O

ffesolution

II 1Large Small

Gage Gage

20 TLC 1H H

III IT IIImixtures pure

off a E'sC C Z n IsortLoad Ignessample

Efe pig pig 599 good

11 11 11 HIT HITHIT HIT HIT H HH H H

Drawdown Drawdown Add draw Fill wsolvent

solvent down solventsmall Runamount column

Page 4: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

E

Hii

HII

iImE cc.Ianarani µ'd

L1

iE

Page 5: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

Paxia has table in appendixcolumnChromatographySamnite 4 a CELIE IE It

mm m7 cop

Thechemists Companion A Handbook of

Practical Data Techniques References

Experimented

Ifluorene fluorenone

50 50 mlm

0.30mL of Sample

El Eadient

0.3g Mixture

11

it 111111111174 Etamis

J 4 3 2 I6

fluorenone fluorene

Page 6: TLC Is · 4/1/2020  · ThechemistsCompanion A Handbook of PracticalData Techniques References Experimented I fluorene fluorenone 50 50 mlm 0.30mL ofSample El Eadient 0.3gMixture

solvent

III

I56


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