A presentation submitted by

Graduated student M.Tech Food Technology

Guided By Prof . Ashish Mohite

VALUE ADDED FOOD PRODUCT BY KALANCHOE PINNATA

S P Gautam

Objective:1.Determine the phytochemical properties

2.Determine the physicochemical properties

3.Development of value added food product by Kalanchoe pinnatum

INTRODUCTION Kalanchoepinnatum(Synonyms:Bryoph

yllum pinnatum, Bryophyllum calycinum). is the perennial herb generally grown in tropical area, southern Africa and America, India, China, and Australia.

Generally this plant is used in herbal medicines to heal injuries, cure disease and is adopted as a herbal remedy in almost all over the world.

The leaves and leaf juice of this plants is considered as folkloric for the curing of kidney stone, gastric ulcer, pulmonary infection, rheumatoid arthritis etc.

Growth and chemical constituents found in kalanchoe pinnata It is a xerophytic plant having thick and

fleshy leaves. It is a popular houseplant. Flowers of this plant arises in the month of Nov-March and fruits in April month.

This plant mature up to height of 1-1.5 m and having four-angled, tall and branched stem and have fleshy dark green color leaves& reddish purple flower.

Leaves are opposite and have simple arrangement of lower leaves and have 7- folio late, long pointed & petioles joined by ridge of stem. It has harsh and sour flavour, sweet in post digestive effect.

The plant contains alkaloids, phenols, flavonoids , tannins, bufadienolides and other chemical compound

Conti… Alkaloids, triterpenes,

bufadienolides,tannins, anthocyanins, glycosides, saponins, sitosterols,quinines, carotenoids, flavonoids, phenolic compound macro elements; magnesium, calcium, potassium, phosphorus, sodium, microelements; iron, zinc, vitamins; niacin, riboflavin, thiamine and ascorbic acid are present in this plant.

Three main components such as bryophyllol, bryophollone and bryophollenone can be extract from fresh leaves of Bryophyllum pinnatum .

Bryophyllol

Syringic acid

Ferulic acid

BufadienolidesFlavonoids

Phenolic compound

Alkaloids

Sitosterol

p-coumaric acid Bryophollone Bryophollenone

Important medicinal properties

Astringent

Analgesic

Anti-aggregant

Anti-dysentric

Anti-inflammatory

Immunomodulatory

Methodology

PHYTOCHEMICAL SCREENING ANALYSIS

.Phytochemicals have two categories:1. Primary2. secondary The phytochemical analysis Commercially

value. Great interest in pharmaceutical companies for the

prod uction of the new drugs for curing of various diseases.

PHYTOCHEMICAL AND PHYSICO-CHEMICALANALYSIS: Steroids (LibermannBurchard’s

Test) Flavoniods.(Lead acetate Test) Saponins.(Foam Test) Carbohydrates.(Molisch’s Test) Tannins.(Ferric chloride Test)

ANALYSIS

Phytochemical Screening Analysis of kalanchoe pinnata.

CHEMICALS REQUIRED Acetic acid Sulfuric acid Lead acetate Fehling solution 1,2 Distrilled water

PROCEDURE

1.TEST OF STEROLS

1mg powder in 1ml acetic acid

Heating to boiling and cooled

Upper layer gives dark green color present of

sterol

Add 1/2 ml of H2So4

2. TEST FOR FLAVONOIDS

Few drop of 10% lead acetate + 0.2g of test

solution

Yellow color precipitate conferm

the flavonoid

3. TEST FOR TANNIN

0.025 mg powder + 2.5ml D water

Added 1.5ml of 10% lead acetate

A bulky white

precipitation occurs present of

tannin

Weigh accurate 2g powder of k.Pinnata

Heating on hot plate to removes fat

or oils

Put in the muffle furnace for 3 Hrs cooled in Dessicator

4. Ash content

5. Moisture content

5g sample Hot air oven at 105c for 3 hrs

Take ready after 1hrs using dessicator

Take the final ready using dessiccator

preparation of barley water

200 gm raw barley

drying (3hrs at 60◦c)

grinding

filter(120 mm seive)

Extract Barley Powder

Prepar ation of leaf kalanchoe pinnata value added food product

1. Making 2 sample 70ml, 120ml, added barley podwer .10g, .g, well mixed for 10 mintutes and then soaking for 24 hrs.

2. After 24 hrs, filter the water.

3. In sample 1, 50 ml of water filter and sample 2, 100 ml of water filter.

4. Then , add the powder of kalanchoe pinnata 5. Sodium benzoate is used as preservative of their

samples.very small amount of preservative used in kalanchoe pinnata juice.

Physicochemical Properties Result

total ash loss of drying pH moisture0

10

20

30

40

50

60

70

Series 1

Series 1

Phytochemical properties of Bryophyllum pinnata result 2

sterol sapponin flavonoid carbohhdrate tannin0

0.2

0.4

0.6

0.8

1

1.2

Series 1

Series 1

Final Result Of Value Added Food Product

water sample preservative0102030405060708090

100

Series 1

Series 2

Chart TitleSeries 1 Series 2

Incorporation of dehydrated leaf mixture powder in various recepies can be helpful for therapeutic purposes.

Kalanchoe's bufadienolides have demonstrated in clinical research to possess antibacterial, anti- tumor, cancer preventative, and insecticidal actions.

Kalanchoe is rich in alkaloids, triterpenes, glycosides,

flavonoids, steroids and lipids.

The leaves contain a group of chemicals called bufadienolides which are very active and have sparked the interest of scientists.

Conclusion

FUTURE ASPECT

Patharchatta leaves

juice extract

Preparation of sugar syrup

Mixing of juice and sugar syrup

Carbonation

Carbonated pinnata drink