TODAYThe SouThern California reSearCh

CenTer for alPD anD CirrhoSiS

10th AnnuAl SympoSium

winTer 2009The Center is supported by grants

from the National Institute on Alcohol Abuse and Alcoholism: P50 AA011999, T32 AA07578,

and R24 AA12885.

inSiDe ThiS iSSue

1 10th Annual Symposium1 Lee Summer Student Fellowship

Program

2 Spotlight on Derick Han2 2009 Pilot Project Award Recipients2 New Grants by Center Members3 Recent Publications by Center Members

ConTaCTShide Tsukamoto Director htsukamo@usc.edu 323-442-5107

rosy Macias Admistrative Manager rmacias@usc.edu 323-442-3109

rebecca handan Secretary handan@usc.edu 323-442-3121

fax: 323-442-3126 www.usc.edu/alpd

The SouThern California reSearCh Center for ALPD and Cirrhosis has hosted its 10th annual symposium on December 5, 2008. This whole day symposium marked the center’s 10th year anniversary since its inception in 1999 and also celebrated the second successful competitive renewal of the NIH/NIAAA P50 center grant totaling $8.1M for 5 years from January 1st, 2009. The symposium showcased three state-of-the-art lectures by George Kunos, Scientific Director of NIAAA/NIH, Peter Tontonoz, Howard Hughes Investigator and Professor of Pathology at UCLA, and Michael Karin, Professor of Pharmacology at UCSD, as well as presentations by the Center investigators and Pilot Project investigators. Samir Zakhari, the Director of the Division of Metabolism and Health Effects of NIAAA, made special remarks on funding opportunities for your investigators.

The center’s scientific advisory board participated in the symposium and evaluated the center program including pilot project applications. The board members who attended the event included Thomas Badger of University of Arkansas, Mark Czaja of Albert Einstein College of Medicine, Jacquelyn Maher of UCSF, and David Whitcomb of University of Pittsburg.

GeorGe KunoS PeTer TonTonoz SaMir zaKhariMiChael Karin

The symposium was followed by a reception at Plaza Market-place where more than 70 center members and friends enjoyed great wine and food including sushi freshly prepared by Mr. Ishito and his assistant from Kappo Ishito. Steve Pandol introduced Carol Dreher, Executive Director for AGA foundation. Elizabeth Fini, Vice Dean of Research Advancement, welcomed the guests and praised the center’s success and the visionary leadership of the center director, Hide Tsukamoto. Abstract awards were presented to four postdoctoral fellows by Vijay Kalra and Kinji Asahina. Hide Tsukamoto closed the event by paying tribute to each center member’s effort which collectively culminated to the center’s success. He also applauded administrative support rendered by Rosy Macias, Rebecca Handan, and Shirley Tsai. w

in SuMMer 2008 The SouThern California Research Center for ALPD and Cirrhosis offered five Student Fellowship Awards under its Annual Lee Summer Student Fellowship Program. The Fellowship Program provided opportunity for the Master and undergraduate students to participate for at least ten weeks in research in the Center investigator’s laboratory and to give a presentation on their research at the center’s progress report meeting in August. They received an $800 Award and a Certificate after completion of the program. ContinueS on next pAge...

lee Summer Student FellowShip progrAm

Noriko Matsushita accepting her abstract award from Kinji Asahina and Vijay Kalra

Spotlight on deriCK hAnDeriCK han iS an aSSiSTanT Professor of Research in the Division of GI/Liver in the Department of Medicine at USC Keck School of Medicine. He obtained B.A. degree in Sociology and Biochemistry from UC Berkeley. During the undergraduate years at Berkeley, he worked in the laboratory of Lester Packer, a pioneer in the field of anti-oxidants. This field fascinated Derick and he has continued in this area. After finishing his undergraduate work, he moved to USC to pursue research under the direction of Enrique Cadenas, a pioneer in the field of free radical biology. In 2001, Derick obtained his Ph.D. and started his post doctoral research in the Center’s training program under the direction

of Neil Kaplowitz, M.D., Professor of Medicine at USC, an authority in the field of glutathione biology and liver toxicity.

Derick has published extensively on the role of glutathione redox status in liver injury and role of C-jun N-terminal kinase in acetoaminophen- induced liver hepatoxicity. To date, he has published 41 papers and 5-chapters in book. His current interest is in redox signaling in alcoholic liver disease. Derick is recipient of several young investigators awards. He received the Young Investigator award from the Oxygen Society and Presidential Fellowship from USC Leadership Research Institute. He is the recipient of Wright Foundation Research Grant from USC, Zumberge Research and Innovation Award and pilot grant from USC Research Center for Liver Research. Based on novel findings on the effect of ethanol on hepatocytes redox signaling, Derick has applied and successfully obtained a KO-1 grant. On the personal side, Derick has developed a passion for acting, as he happens to be living in Hollywood. There he met aspiring actor Tracy. Derick got married to Tracy in September 2007, and they are living happily thereafter. w

This year, we presented the awards to these following students: Sarah ahmadi, (ProjeCT TiTle: Post-translational protein redox changes during alcoholic liver disease) whose mentor was Derick Han & Neil Kaplowitz; lewei Duan (ProjeCT TiTle: TLR4 and hepatocarcinogenesis: Synergistic interactions between alcohol and Hepatitis C virus) whose mentor was Keigo Machida; Yang li (ProjeCT TiTle: The effect of PTEN on mitochondrial biogenesis in the damage liver caused by alcohol and obesity) whose mentor was Bangyan Stiles; Polina Mareninova (ProjeCT TiTle: Effect of ethanol feeding on autophagy in pancreas), whose mentor was Anna Gukovskaya & Stephen Pandol; Yizhou zhu (ProjeCT TiTle: Adams and TIMPs by hepatic stellate cells), whose mentor was Yuan Ping-Han & Lan Qin. w ToP row: Yang Li, Polina Mareninova, Sarah Ahmadi, Yizhou Zhu, Keane Lai. BoTToM row: Lewei Duan and Samantha Yeligar

CongrAtulAtionS to: 2009 pilot projeCt AwArd reCipientSKinji asahina, Ph.D., uSC, Hepatic Stellate Cell Precursors in Developing and Regenerating Livershyeongnam (joe) jeong, Ph.D., uSC, Identification of Novel Transforming Genes in Hepatocellular Carcinomaekihiro Seki, M.D., Ph.D., uCSD, TLR4 Regulates Activation of Hepatic Stellate Cells and Kupffer Cells in Alcoholic Liver FibrosisBernd Schnabl, M.D., uCSD, Early Bacterial Translocation as Mechanism for Alcohol-Induced Fibrosis Progressionjingzhen (jenny) Yuan, Ph.D., uCla, The Role of Protein Kinase D1 in Alcohol-Induced Pancreatitis

new grAntS by Center memberSLaurie D. DeLeve, 2R01 DK606423-05A2, Determinants of Sinusoidal Endothelial Cell Phenotype, 5/1/08 – 6/30/12Derick Han, 1K01 AA016911-01A2, Thiol Redox Modulation of NF-kB Pathway in Alcoholic Liver Injury, 12/1/08 – 11/30/12Neil Kaplowitz, 1R21 AA017746-01, Retrograde Signaling In Alcohol-Induced Mitochondrial Stress and Biogenesis, 9/15/08 – 8/31/10Keigo Machida, Wright Foundation/USC Institutional Fund, AP1 and DNA Damage-Repair in HCV-Induced Hepatocellular Carcinoma, 7/1/08 – 6/30/09Komal Ramani, K99/R00 NIH Pathway to Independence Award, Methionine Adenosyl Transferase Genes as Therapeutic Targets for Liver Fibrosis, 4/1/09 – 3/31/13Hamid M. Said, R01 AA018071, Mechanisms of Pancreatic Thiamin Uptake: Effect of Alcohol, 1/1/09 – 12/31/13

ContinueS From previouS pAge...

2 www.usc.edu/alpd

www.usc.edu/alpd 3

1. Adachi M, Brenner DA. High molecular weight adiponectin inhibits proliferation of hepatic stellate cells via activation of adenosine monophosphate-activated protein kinase. Hepatology 47(2):677–85, 2008.

2. Asahina K, Tsai S, Li P, Ishii M, Maxson RE, Sucov HM, Tsukamoto H. Mesen-chymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development. Hepatology In press, 2008

3. Bardag-Gorce F, Oliva J, Villegas J, Fraley S, Amidi F, Li J, Dedes J, French B, French SW. Epigenetic mechanisms regulate Mallory-Denk body formation in the livers of drug-primed mice. Exp Mol Pathol 84(2):113–21, 2008.

4. Brandl K, Plitas G, Mihu CN, Ubeda C, Jia T, Fleisher B, Schnabl B, DeMatteo RP, Pamer EG. Vancomycin-resistant enterococci exploit antibiotic-induced innate immune deficits. Nature 455(7214):804–7, 2008.

5. Cheng JH, She H, Han YP, Wang J, Xiong S, Asahina K, Tsukamoto H. Wnt antagonism inhibits hepatic stellate cell activation and liver fibrosis. Am J Physiol Gastrointest Liver Physiol 294(1):G39–49, 2008.

6. Chung C, Shugrue C, Nagar A, Doll JA, Cornwell M, Gattu A, Kolodecik T, Pandol SJ, Gorelick F. Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. Gastroenterology 2008.

7. DeLeve LD, Wang X, Guo Y. Sinusoidal endothelial cells prevent rat stellate cell activation and promote reversion to quiescence. Hepatology 48(3):920–930, 2008.

8. DeLeve LD, Wang X, Kanel GC, Atkinson RD, McCuskey RS. Prevention of hepatic fibrosis in a murine model of metabolic syndrome with non-alcoholic steatohepatitis. Am J Pathol 173(4):993–1001, 2008.

9. De Minicis S, Seki E, Oesterreicher C, Schnabl B, Schwabe RF, Brenner DA. NADPH oxidase mediates fibrotic and inflammatory effects of leptin on hepatic stellate cells. Hepatology 48(6):2016–26, 2008.

10. De Minicis S, Seki E, Oesterreicher C, Schnabl B, Schwabe RF, Brenner DA. Reduced nicotinamide adenine dinucleotide phosphate oxidase mediates fibrotic and inflammatory effects of leptin on hepatic stellate cells. Hepatology 48(6):2016–26, 2008.

11. Edderkaoui M, Odinokova I, Ohno I, Gukovsky I, Go VL, Pandol SJ, Gukovskaya AS. Ellagic acid induces apoptosis through inhibition of nuclear factor kappa B in pancreatic cancer cells. World J Gastroenterol 14(23):3672–80, 2008.

12. Fu Y, Zhou YJ, Lu SC, Chen AP. Epigallocatechin-3-gallate inhibits growth of activated hepatic stellate cells by enhancing the capacity of glutathione synthesis. Mol Pharmacol 73:1465–73, 2008.

13. Gäbele E, Mühlbauer M, Dorn C, Weiss T, Froh M, Schnabl B, Wiest R, Schölmerich J, Obermeier F, Hellerbrand C. Role of TLR-9 in hepatic stellate cells and experimental liver fibrosis. Biochem Biophys Res Comm 376(2):271–6, 2008.

14. Gordeuk VR, Diaz SF, Onojobi GO, Kasvosve I, Debebe Z, Edossa A, Pantin JM, Xiong S, Nekhai S, Nouraie M, Tsukamoto H, Taylor RE. Ferroportin Q248h, dietary iron, and serum ferritin in community African-Americans with low to high alcohol consumption. Alcohol Clin Exp Res 32(11):1947–53, 2008.

15. Gukovsky I, Lugea A, Shahsahebi M, Cheng JH, Hong PP, Jung YJ, Deng QG, French BA, Lungo W, French SW, Tsukamoto H, Pandol SJ. A rat model reproducing key pathological responses of alcoholic chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol 294(1):G68–79, 2008.

16. Hanawa N, Shinohara M, Saberi B, Gaarde WA, Han D, Kaplowitz N. Role of JNK translocation to mitochondria leading to inhibition of mitochondria bioenergetics in acetaminophen-induced liver injury. J Biol Chem 283(20):13565–77, 2008.

17. Iglesias Ara A, Xia M, Ramani K, Mato JM, and Lu SC. S-adenosylmethionine inhibits lipopolysaccharide-induced gene expression via modulation of histone methylation. Hepatology 47:1655–1666, 2008.

18. Ji C, Shinohara M, Vance D, Than TA, Ookhtens M, Chan C, Kaplowitz N. Effect of transgenic extrahepatic expression of betaine-homocysteine methyltransferase on alcohol or homocysteine-induced fatty liver. Alcohol Clin Exp Res 32(6):1049–58, 2008.

19. Kaplowitz N, Shinohara M, Liu ZX, Han D. How to protect against acetaminophen: Don’t ask for JUNK. Gastroenterology 135(4):1047–51, 2008.

20. Kisseleva T, Brenner DA. Fibrogenesis of parenchymal organs. Proc Am Thorac Soc 5(3):338–42, 2008.

21. Kisseleva T, Brenner DA. Mechanisms of fibrogenesis. Exp Biol Med (Maywood) 233(2):109–22, 2008.

22. Ko KS, Yang HP, Noureddin M, Iglesia-Ara A, Xia M, Wagner C, Luka Z, Mato JM, Lu SC. Changes in S-adenosylmethionine and glutathione homeostasis during endotoxemia in mice. Lab Invest 88:1121–1129, 2008.

23. Kodama Y, Brenner DA. c-Jun N-terminal kinase signaling in the pathogenesis of nonalcoholic fatty liver disease: Multiple roles in multiple steps. Hepatology 49(1):6–8 2009.

24. Kondo Y, Machida K, Liu M, Ueno Y, Kobayashi K, Wakita T, Shimosegawa T, Lai MM. Hepatitis C virus infection of T cells inhibits proliferation and enhances FAS-mediated apoptosis of T cells by down-regulating CD44v6 expression. J Infec Dis In press, 2008.

25. Lai CK, Jeng KS, Machida K, Lai MM. Association of hepatitis C virus replication complexes with microtubules and actin filaments is dependent on the interaction of NS3 and NS5A. J Virol In press, 2008.

26. Lee JK, Edderkaoui M, Truong P, Ohno I, Jang KT, Berti A, Pandol SJ, Gukovskaya AS. NADPH oxidase promotes pancreatic cancer cell survival via inhibiting JAK2 dephosphorylation by tyrosine phosphatases. Gastroenterology 133(5):1637–48, 2007.

27. Li J, Bardag-Gorce F, Dedes J, French BA, Amidi F, Oliva J, French SW. S-adenosylmethionine prevents Mallory Denk body formation in drug-primed mice by inhibiting the epigenetic memory. Hepatology 47(2):613–24, 2008.

28. Lu SC, Mato JM. S-adenosylmethionine in cell growth, apoptosis and liver cancer. J Gastroenterol Hepatol 1:S73–7, 2008.

29. Machida K, Kondo K, Hwang J, Chen Y, Cheng KT, Keck Z, Foung S, Dubuisson J, Sung VM, Lai

MM. HCV-induced immunoglobulin hypermutation reduces the affinity and neutralizing activities of antibodies against HCV envelope protein and antibody-dependent cell-mediated cytotoxicity. J Virol In press, 2008.

30. Machida K, Tsukamoto H, Mkrtchyan H, Duan L, Dynnyk A, Liu H, Wang J, Ray R, Ray R, Ou J, Seki E, Lai MM. Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatocellular carcinoma: The role of stem cell marker nanog. Proc Natl Acad Sci U S A In press, 2008.

31. Martinez-Chantar ML, Vázquez-Chantada M, Ariz U, Martínez N, Varela M, Luka Z, Capevila A, Rodriguez J, Aransay AM, Matthiesen R, Yang HP, Calvisi DF, Esteller M, Fraga M, Lu SC, Wagner C, Mato JM. Loss of the GNMT gene leads to steatosis and hepatocellular carcinoma in mice. Hepatology 47:1191–1199, 2008.

32. Martínez-López N, Varela-Rey M, Ariz U, Embade N, Vazquez-Chantada M, Fernandez-Ramos D, Gomez-Santos L, Lu SC, Mato JM, Martinez-Chantar ML. S-adenosylmethionine and proliferation: New pathways, new targets. Biochem Soc Trans 36:848–852, 2008.

33. Mato JM, Martínez-Chantar ML, Lu SC. Methionine metabolism and liver disease. Ann Rev Nutr 28:273–293, 2008.

34. Miura K, Taura K, Kodama Y, Schnabl B, Brenner DA. Hepatitis C virus-induced oxidative stress suppresses hepcidin expression through increased histone deacetylase activity. Hepatology 48(5):1420–9, 2008.

35. Morgan K, Uyuni A, Nandgiri G, Mao L, Castaneda L, Kathirvel E, French SW, Morgan TR. Altered expression of transcription factors and genes regulating lipogenesis in liver and adipose tissue of mice with high fat diet-induced obesity and nonalcoholic fatty liver disease. Eur J Gastroenterol Hepatol 20(9):843–54, 2008.

36. Odinokova IV, Sung KF, Mareninova OA, Hermann K, Evtodienko Y, Andreyev A, Gukovsky I, Gukovskaya AS. Mechanisms regulating cytochrome c release in pancreatic mitochondria. Gut 2008.

37. Odinokova IV, Sung KF, Mareninova OA, Hermann K, Gukovsky I, Gukovskaya AS. Mitochondrial mechanisms of death responses in pancreatitis. J Gastroenterol Hepatol 23 Suppl 1:S25–30, 2008.

38. Oliva J, Bardag-Gorce F, French BA, Li J, McPhaul L, Amidi F, Dedes J, Habibi A, Nguyen S, French SW. Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis. Exp Mol Pathol 84(2):102–12, 2008.

39. Oliva J, Bardag-Gorce F, Li J, French BA, Nguyen SK, Lu SC, French SW. Betaine prevents Mallory-Denk body formation in drug-primed mice by epigenetic mechanisms. Exp Mol Pathol 2008.

40. Oliva J, French BA, Li J, Bardag-Gorce F, Fu P, French SW. Sirt1 is involved in energy metabolism: The role of chronic ethanol feeding and resveratrol. Exp Mol Pathol 85(3):155–9, 2008.

41. Ramani K, Yang HP, Xia M, Iglesias Ara A, Mato JM, Lu SC. Leptin’s mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2b. Hepatology 47:521–531, 2008.

42. Rountree CB, Senadheera S, Mato JM, Crooks GM, and Lu SC. Expansion of liver cancer stem cells during aging in methionine adenosyltransferase 1A deficient mice. Hepatology 47:1288–1297, 2008.

43. Saberi B, Shinohara M, Ybanez MD, Hanawa N, Gaarde WA, Kaplowitz N, Han D. Regulation of H(2)O(2)-induced necrosis by PKC and AMP-activated kinase signaling in primary cultured hepatocytes. Am J Physiol Cell Physiol 295(1):C50–63, 2008.

44. Schnabl B, Brandl K, Fink M, Gross P, Taura K, Gäbele E, Hellerbrand C, Falk W. A TLR4/MD2 fusion protein inhibits LPS-induced pro-inflammatory signaling in hepatic stellate cells. Biochem Biophys Res Comm 375(2):210–4, 2008.

45. Schnabl B, Scholten D, Brenner DA. What is the potential role of antifibrotic agents for the treatment of liver disease? Nat Clin Pract Gastroenterol Hepatol 5(9):496–7, 2008.

46. Seki E, Brenner DA. Toll-like receptors and adaptor molecules in liver disease: update. Hepatology 48(1):322–35, 2008.

47. Shukla SD, Velazquez J, French SW, Lu SC, Ticku MK, Zakhari S. Emerging role of epigenetics in the actions of alcohol. Alcohol Clin Exp Res 32(9):1525–34, 2008.

48. Sir D, Chen WL, Choi J, Wakita T, Yen TS, Ou JH. Induction of incomplete autophagic response by hepatitis C virus via the unfolded protein response. Hepatology 48(4):1054–61, 2008.

49. Taura K, De Minicis S, Seki E, Hatano E, Iwaisako K, Osterreicher CH, Kodama Y, Miura K, Ikai I, Uemoto S, Brenner DA. Hepatic stellate cells secrete angiopoietin 1 that induces angiogenesis in liver fibrosis. Gastroenterology 135(5):1729–38, 2008.

50. Thrower EC, Osgood S, Shugrue CA, Kolodecik TR, Chaudhuri AM, Reeve JR Jr, Pandol SJ, Gorelick FS. The novel protein kinase C isoforms -delta and -epsilon modulate caerulein-induced zymogen activation in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 294(6):G1344–53, 2008.

51. Tsukamoto H, She H, Hazra S, Cheng J, Wang J. Fat paradox of steatohepatitis. J Gastroenterol Hepatol 23 Suppl 1:S104–7, 2008.

52. Tsukamoto H, Mkrtchyan H, Dynnyk A. Intragastric ethanol infusion model in rodents. Methods Mol Biol 447:33–48, 2008.

53. Xiong S, She H, Zhang AS, Wang J, Mkrtchyan H, Dynnyk A, Gordeuk VR, French SW, Enns CA, Tsukamoto H. Hepatic macrophage iron aggravates experimental alcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 295(3):G512–21, 2008.

54. Yang HP, Iglesias Ara A, Magilnick N, Xia M, Ramani K, Chen H, Lee TD, Mato JM, Lu SC. Expression pattern, regulation and function of methionine adenosyltransferase 2β alternative splicing variants in hepatoma cells. Gastroenterology 134:281–291, 2008.

55. Yang HP, Magilnick N, Xia M, Lu SC. Effects of hepatocyte growth factor on glutathione synthesis, growth, and apoptosis is cell density-dependent. Exp Cell Res 314:398–412, 2008.

56. Yu MC, Yuan JM, Lu SC. Alcohol, cofactors and the genetics of hepatocellular carcinoma. J Gastroenterol Hepatol 1:S92–7, 2008.

57. Yuan J, Lugea A, Zheng L, Gukovsky I, Edderkaoui M, Rozengurt E, Pandol SJ. Protein kinase D1 mediates NF-B activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 295:G1190–G1201, 2008.

58. Yuksek K, Chen WL, Chien D, Ou JH. Ubiquitin-independent degradation of hepatitis C virus F protein. J Virol 83(2):612–21, 2009.

reCent publiCAtionS by Center memberS

The Southern California research Center for alPD and CirrhosisKeck School of Medicine of theUniversity of Southern California1333 San Pablo Street, MMR 4th FloorLos Angeles, CA 90033www.usc.edu/alpd

11th Annual lecture Series on Cellular homeostasisthurSdAyS: 12:00 – 1:30 pm: mCKibben leCture hAll, room 156, USC

Feb. 5regulation of the Cation Coupled Chloride Cotransporters (Slc12 Family), Gerardo Gamba, University of Mexico

Feb. 12induction of Coronary vascular progenitor Cells by liver bud-derived Signal, Takashi Mikawa, University of California San Francisco

Feb. 19the role of inflammation on insulin resistance and type 2 diabetes, Jerrold M. Olefsky, University of California San Diego

Feb. 26ryk mediated wnt Signaling during neurogenesis, Wange Lu, University of Southern California

mar. 5mining the Function of Stress-inducible Chaperone grp78/bip through the use of mouse models, Amy Lee, University of Southern California

mar. 12A novel role for Caspase-8 in initiating a wound healing response, Colin Jamora, University of California San Diego

mar. 26life in the Fast lane—rapid traffic of Sodium transporters maintains volume and blood pressure homeostasis, Alicia McDonough, University of Southern California

Apr. 2hepatic innate immune regulation that Controls hepatitis C virus, Takeshi Saito, University of Washington School of Medicine

Apr. 16ppArs, macrophage Activation and insulin resistance, Ajay Chawla, Stanford University School of Medicine

4th internAtionAl SympoSium on Alpd & CirrhoSiS

oCtober 1 & 2, 2009: hurghAdA, egypt

Organizers:gamal esmat, m.d.

email: gesmat@gamalesmat.comScott l. Friedman, m.d.

email: scott.friedman@mssm.edu

SAve the dAte6th Annual Community Seminar

Friday, April 3, 20098:30 am – 1:00 pm

Alhambra Campus Auditorium

uSC institute forhealth promotion &

disease prevention research

1000 South Fremont AvenueAlhambra, CA 91803

to register Contact:Alexandra bruehl(626) 299-4502

abruehl@ph.lacounty.govmuSt pre-regiSter

Free!!!