TRAINING

EXOSSINE™: EXO-P™

DETOXIFYING-ANTI-POLLUTION

• Introduction on protection, claims & consumers

• Exo-P: Protection

– In vitro assay (heavy metal chelation & UV protection)

– Ex vivo assay (pollution protection)

– Anti-pollution consumer test

• Toxicological studies

• Applications

• Conclusion

Exossine Technical Agenda

What are we protecting our skin from?

Before

• UV

• Aging process

• Skin sensitivity & intolerance

Now

• Elements (extreme weather, pollution, heavy metals)

• Our own physiological changes (hormones)

• Man-made factor (electromagnetic waves, cigarette)

We protect ourselves from things that make us look bad & unhealthy

Effect of pollution on skin

Increases the oxidation of sebum, pimple formation = acne

Rashes, allergies & skin sensibility

Interferes with the skin ability to regulate moisture = dehydration

Takes out the oxygen from the skin cells = dull & saggy skin

Increases free radicals and the effect of UV radiation

Complexion becomes grayish

Premature aging!

Decrease collagen & elastin production = rough skin & fine lines

Skin cells do not have proper defenses against man-made pollution

Market Info

• Since January 2008: 4624 products were launched with the «Protect against harsh elements, pollution, smoke, environmental aggressors, urban damage claim»

• Important evolution since 2008.

Evolution since 2008 Product segmentation

+40%

40%

Global Protection Market

Consumer Info • Who buys «Protective products» and why?

- Consumers who have sensitive skin and reactive skin. Why? To reduces or minimize unwanted side effect of sensitive & reactive skin

(redness, irritation, etc).

- Consumers who live in big polluted cities and who desire a maximum skin protection from pollution, sun and other elements.

Why? To minimize as much as possible skin damage and reduce dull skin that come with skin submitted to pollution.

- Consumers who have a preventive approach to skin aging and photo-protection (suncare).

Why? By reinforcing skin defenses it will help fight the skin aging process and maintain as long as possible a youthfull appearance.

Global Protection Market

Global Protection Application

Clarins Expertise 3P Screen Mist

Anti-Electromagnetic Waves & Urban Pollution

Clinique Comfort On Call Allergy Tested Relief Cream protects skin against wind and cold; said to prevent pollen allergies

DoshaCare Environmental Shield

helps to neutralize the effects of smoke, smog and surface radicals

SkinMedica TNS Ultimate Daily Moisturizer

fight the visible signs of environmental damage resulting from UV light, pollutants,

and harmful chemicals

Many Different Applications

• Moisturizing care & radiant skin care • Protective care • Sensitive skin • Daily care • Sun care • Hair care

What we are offering: Exo-P

Exotic expolysaccharide and sustainable active that provides protection against toxic chemicals, UV, heavy metals, cigarette

smoke, fuel exhaust and urban pollution!

Reality…

• Human activities

• Urban pollution

• Sun exposure

• Cigarette smoke

Affect everybody!!!

Cause loss of skin hydration, protection, dull skin complexion, skin sensitivity…

Lucas Meyer Cosmetics Innovations found the solution in a hidden paradise of French Polynesia…

French Polynesia a hidden beauty beyond the ordinary…

French Polynesia are volcanic islands and atolls located in

the eastern South Pacific

Moorea Island called the «Magical Island» is an atoll

Atoll is an island of coral that encircles a lagoon

Volcano

Corals that grow on the flanks of the volcano

Exotic ExoPolySaccharides

The salty water of Moorea lagoon are the site of Kopara Kopara are unique ecosystem constituted of microorganisms only To protect themselves from UV, pH & salinity variations, Kopara microorganisms produce exopolysaccharides (EPS) as protective shield What is an exopolysaccharide vs polysaccharides?!?

Polysaccharides: Polymers that are composed of repeated sugar.

Example of polysaccharide: cellulose, chitine, cell membrane constituants of yeast Exopolysaccharides: Polymers that are composed of sugar and are secreted by a microorganism into the surrounding environment.

Lucas Meyer Cosmetics has isolated mircoorganisms producing EPS:

Vibrio alginolyticus subspecies 245 (Exo-T)

Alteromonas macleodi subspecies 203 (Exo-H)

Alteromonas macleodi subspecies 229 (Exo-P)

Exclusive & Patented!

Sustainable ExoPolySaccharides

EPS are obtained through a proprietary green fermentation process

This process allows us to reproduce the Kopara ecosystem

without damaging the natural resources & disrupting the

biodiversity

EPS are produced in French Polynesia

to help maintain the local economy

The Exossine System: Protection - Hydration - Texture

Exo-P™ Product composition

Exo-P Protection

1000 kDa

Produced by Alteromonas macleodi subsp 229

Ramified polysaccharide composed: -57% neutral sugars (glucose, galactose, mannose)

-25% uronic acid (glucuronic acid, galacturonic acid)

-8% sulphates

Exo-P is formulated in butylene glycol and water without preservative

EPS are rich in essential minerals like: sodium, potassium, calcium & magnesium

Sodium: Detoxification & maintenance water balance Potassium: Balances healthy skin water balance

Calcium: Protects skin tissues, induces cell differentiation for a better skin barrier function Magnesium: Stimulates cell regeneration, energy production and is an anti-stress agent

- Confidential -

Exo-P the Natural Protector

In vitro & Ex vivo tests

Heavy metals chelation process

Chelating agent

Heavy Metal

Heavy metal chelation = Heavy metal no longer toxic & cannot penetrate the skin

Chelation:

The use of chelating agents to detoxify dangerous metal by converting them to a chemically inert form that can be eliminated

Effect on heavy metals chelation

Test Protocol: • Exo-P was incubated with heavy metals Cadmium (Cd) or Lead (Pb) for 3hrs

• Centrifugation

• Concentration in Cd and Pd in supernatant by flame atomic absorption spectrometry (FAAS)

Cadmium is known to cause DNA damages and has been associated with alopecia (hair loss) and serious skin rash Lead increases oxidative stress and skin lipid peroxidation

Exo-P protects against toxic chemicals and thus limits skin damages caused by pollution

Exo-P has the ability of chelating cadmium and lead

Effect of Exo-P on heavy metals chelation

Cadmium

0

20

40

60

80

100

120

140

160

180

0 100 200 300 400 500 600

[Cd] eq (mg/L)

q C

d a

bso

rbé

(mg/

g d

'EP

S)

Lead

0

50

100

150

200

250

300

0 100 200 300 400 500 600

[Pb]eq (mg/l)

q P

b r

eten

u (

mg/

g d

'EP

S)

free-radicals production (ROS)

DNA alterations

Lipid peroxidation Collagen fibers degradation

Proteins oxidation

Skin Aging

UV Stresses (internal / external)

Pollution

Free Radical Cascade

Test Protocol:

• Human keratinocytes incubated with Exo-P or positive control (BHA) for 24hrs

Butylated hydroxyanisole (BHA) is a well-known antioxidant added to foods to preserve

fats

• Cells were irradiated with UVB

• Lipid peroxidation is identified with a specific fluorescence probe and quantified by

cytometry

Effect on UV-induced lipid peroxidation

Exo-P protects and prevents UV-induced skin damage to limit premature aging caused by sun exposure

Exo-P reduces lipid peroxidation in a dose-

dependant manner

Effect on UV-induced lipid peroxidation

Test Protocol:

• Lotion containing 3% Exo-P was applied on skin explants surface for 4 days

• On day 4 addition of filter paper containing a mixture of heavy metals & hydrocarbons (found in fuel exhaust & cigarette smoke) on skin explants surface

• On day 5 removal of filter paper and MDA quantification & histological observations Malondialdehyde (MDA) = end product of lipid peroxidation used as biomarker of oxidation in

damaged skin

Anti-pollution effect on skin explants

Aluminium (Al)

Arsenic (As)

Cadnium (Cd)

Chromium (Cr)

Mercury (Hg)

Nickel (Ni)

Lead (Pb)

Phosphorus (P)

Tellurium (Te)

20 mg/L 20 mg/L 2 mg/L 20 mg/L 20 mg/L 5 mg/L 20

mg/L 10 mg/L 20 mg/L

Hydrocarbons content: benzene, toluene, xylene, anthracene, naphtol

Exo-P protects against urban pollution and thus can be used to reduce skin sensitivity and allergic reactions

caused by pollution

Exo-P reduces the production of MDA by 44%

under urban pollution conditions

Anti-pollution effect on skin explants

Control Skin + pollutant

Exo-P protects and reduces pollution damage on skin

Exo-P prevents the formation of pycnotic nuclei and improves dermal-epidermal cohesion

Dermal-epidermal separation = Lack of cohesion

Pycnotic nuclei = Severe cell damage which lead to cell

death

Skin + Pollutant + Exo-P™

SC

Epidermis

DEJ

Dermis

Anti-pollution effect on skin explants

No pycnotic nuclei

Reduced collagen network = Desease skin support

Normal collagen network

Exo-P the Natural Protector

Consumer test

Anti-pollution consumer test

Test Protocol:

• 57 consumers (age: 30 to 50) living in a big city & 50% are smokers (29 out of 57)

• Application of a cream with 2% Exo-P in the morning & at night for a period of 7 days

Consumers agree that Exo-P makes their skin more resistant to aggressions and improves dull skin for a healthy skin complexion

Toxicological Studies

• Cytotoxicity (maximal dose: 30%)

• HET CAM (maximal dose: 30%)

• AMES Test (maximal dose: 30%)

• Acute skin tolerance (single patch test) (maximal dose: 30%)

• HRIPT (irritation and sensitization) (maximal dose: 30%)

• Photosensitization (maximal dose: 30%)

Excellent Safety Profile

Products Efficacy Summary

In vitro Ex vivo Clinical

Exo-P Protection

-Heavy metals chelation

-Protection against UV-induced lipids peroxidation

-Protection against pollutants (cigarette smoke & fuel exhaust)

Anti-pollution consumer test

-Improves skin dullness, resistance to aggressions & climatic changes for a luminous & healthy skin

Exo-H Hydration

-Stimulation of hyaluronic acid secretion

-Induction of total lipid synthesis

-Protection against delipidation

-Induction of filaggrin expression (fast action! 3hrs)

Combined test:

-Induce skin hydration

-Improve skin texture

Exo-T Texture

-Induction of desquamation markers with higher efficacy than retinoic acid

-Induction of skin regeneration

-Protection against delipidation

-Induction of transglutaminase expression

Complete protection & moisturizing and restructuring effect leading to a healthy, smooth & radiant skin complexion

New results coming soon!

Exo-PTM Protects against free radicals, toxic chemicals, heavy metals and urban pollution

Exo-HTM

Boosts natural

hydrating functions by stimulating hyaluronic acid, fillagrin and lipids

Exo-TTM

Stimulates desquamation and

differentiation markers and induction of TGK

expression for maximal skin renewal

Products Efficacy Summary

Exossine™ Applications

Recommended dosage: 1-3%

•Moisturizing care •Detox skin care •Anti-stress care •Protective care •Daily care •Anti-aging care •Skin repair treatment •Sun care •Body care •Cosmeceutical care •Spa care •Men care •Hair care •Urban care

Anti-Pollution Daily Cream

2% Exo-P: pollution protection

1% Exo-H: moisturizing effect

0.5% Homeo-Shield: shielding effect

Super Detoxifying serum 2% Exo-P: anti-pollution effect

1% Aldavine: anti-inflammatory

0.01% EUK-134: neutralizes free radical

Purifying Anti-Pollution mask

1% Exo-P: detox & improves dullness

1% Exo-T: refines skin texture

1% CW: calms the skin

Potential Applications:

Super sensitive skin cream

2% Exo-P: protects against urban pollution

0.3% Neutrazen: reduces sensitivity threshold

0.5% Abyssine: calms the skin

Formulation Tips

Exo-P is an aqueous gel easy to work and formulate with Exo-P can be added a room temperature or in the cooling phase at the end of the formulating process Exo-P can easily be incorporated in various aqueous formulas (emulsion O/W, lotion, foaming base, etc...) Exo-P will add a soft, light and creamy touch to the formula. No known incompatibilities pH: 4 - 8

Features Benefits

Actives produced by microorganisms using a green fermentation process

Offer Sustainable & Eco-friendly actives

Supply Renewable source of ingredients

Exotic exopolysaccharides rich

in minerals

Give the opportunity to explore new marketing avenues

Multi-functional (protection & improve formulating properties)

Protective effect of Exo-P Provides complete protection against urban pollution and UV

Positive consumer perception after only 7 days

Makes the skin more resistant to agressions and improves dull skin for healthy & luminous skin

Exossine™ Features & Benefits

Company / Product

Origin Application In vitro / Ex vivo Clinical %

Lucas Meyer Cosmetics /

Exossine™

Exo-P

Exopolysaccharide obtained by Kopara microorganisms from French Polynesia

Skin protection

Anti-pollution

Skin radiance

Cadmiun & lead chelation

0.1%: ↑ protection against UV

3%: ↑ protection against fuel exhaust & cigarette smoke (ex vivo)

2% Exo-P: ↑ skin protection & resistance = luminous, healthier & radiant look (7 days)

1-3%

Alpaflor/

Nectapure

Mix of plant extracts Treatment against city stress

Anti-oxidant 5%: ↑ moisturization (corneometry)

3-5%

L. Serobiologique /

Purisoft

Seed from Indian tree Anti-pollution Protection from cadmium & mercury

3% : protects from cigarette smoke

Skin cleansing: +62% vs placebo

1-3%

L. Serobiologique /

Egyptyl

Wheat seed extract

Protection from urban pollution

0.1 - 0.3 - 1%: ↑ cell growth

Protects against exhaust gas

1-3%

Sederma / Venuceane

Biotech product obtained from bacterial fermentation

(enzymes cocktail)

Anti-aging

Anti-oxidant

3%: ↓ peroxidation & protection from lipid peroxidation

3%: protect DNA from UVA

3% ↑ protection from UVA

3% ↓ peroxidation

3% ↓ TEWL & ↑ hydration (56d)

5% ↑ hydration & texture (6mths)

3%

Solabia /

Glycofilm

Polysaccharide obtained by bacterial fermentation

Second skin (protection)

5%: ↑ cytoprotection from metal (cadmium)

1%, 3% & 5%: protection from carbon particles

3% & 5%: boost SPF

3-5%

Sederma /

Osmopur

ivy, sunflower and rice bran on an acrylic polymer & polyol base

Protects from pollution

5%: ↓ citotoxicity to cigarette smoke

3%: ↑ protection against exhaust

fumes & heavy metals (cadmium)

Seppic / Manoliva Olive extract +manganes gluconate

Anti-pollution 6ppm: ↑ protection from cigarette smoke & peroxidation (↑ than vit C)

0.024%: anti-oxidant

3%

The Competition Origin

Fast action

Dosage

Exo-P: Retail: 209$/kg Retail: 141€/kg (2.82 €/kg) Distributor: 156$/kg Distributor: 105€/kg

What you get:

New & exotic polysaccharides

Innovative and patented product

Well defined active obtained through green process (fermentation)

Low recommended dosage range: 1 to 3%

Fast results (after 7 days)

Exossine™ Pricing

Lanacityn PF : 138$/kg - 95€/kg Distributor price Exo-H: 202$/kg - 134€/kg Exo-T: 236$/kg - 157 €/kg

Exo-P™ is an innovative

active providing complete protection

Exo-P™ Summary

Innovative & unique actives from exotic ecosystem

(we do not harm the local environment)

Provides complete protection

Complementary action with clinically proven efficacy to

induce long-term hydration & improve skin texture for a

smooth skin and radiant complexion!