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Troubleshooting
Edwin A. Burgstaler, MT., HP. (ASCP)
ASFA 2015 Annual Meeting May, 2015
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Troubleshooting Objectives
• The participant will recognize some problems in apheresis procedures and their possible causes.
• The participant will be able to cite examples of techniques to address troubles with apheresis procedures.
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Apheresis Troubleshooting
Pump Troubleshooting Roller Pump
Dirty rollers
Slippery rollers
Poor tubing tension
Wrong tubing diameter or platen clearance
Poor roller tension, broken or weak spring
Rotor slipping on shaft
Obstruction in pump head
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Apheresis Troubleshooting
Pump Troubleshooting Pneumatic Pump
Pump not properly seated
Poor air pressure
Compressor is faulty
Rubber shield out of place
Kit leaks or defects
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Apheresis Troubleshooting
Clamp Troubleshooting
Dirty and sticky due to spills
Poor spring tension
Poor electric signal or air pressure
Wrong size tubing
Stacked tubing
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Apheresis Troubleshooting
Machine Components-Air Detectors
Most are ultra-sonic
Level sensors usually optical with light transmission
Very sensitive
Foam can be a problem
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Apheresis Troubleshooting
Air Detector Troubleshooting
Need very good contact with sensor
Clean the sensor
Load properly- “floss in”
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Apheresis Troubleshooting
Machine Components- Electronic Scales
Very sensitive-handle with care
Don’t use as a miscellaneous hanger at any time
Need to be tarred (zero)
Watch for obstructions
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Apheresis Troubleshooting
Centrifuge Troubleshooting
Need to be installed properly
Prevent multi-lumen tube breakage
Proper separation
Good balance
Air locks are possible
Difficult to remove
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Apheresis Troubleshooting High Centrifuge Pressure
Possibilities:
Strangulated or pinched tubing
Air in centrifuge-leak or introduced
Clot in centrifuge
Kinked tubing beyond centrifuge, kit defect
Poor return access
Concentrated RBC
High viscosity fluid-protein, HES replacement
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Apheresis Troubleshooting
Machine Components- Pressure Sensors
Measure positive, negative, or both pressures
Need a good seal of sensor and pod
Need a baseline reading (room air pressure)
Some have protective filters for circuit boards
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Apheresis Troubleshooting
Pressure Troubleshooting
Poor seal of sensor and pod
Kink in line
Leak in kit
Protective filter is wet
Defective sensor or pod
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Apheresis Troubleshooting
Pressure Troubleshooting
• Access pressure low
• Causes: kinks, clots, bore and needle size, vein position, collapsing vein, stress of patient/donor, faulty sensor
• Remedy: check the line from inlet pump to access site only, correct problems, apply heat, slow down, reassure patient/donor
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Apheresis Troubleshooting
Pressure Troubleshooting
• Return pressure high
• Causes: kinks, vein position, bore and needle size, clots, clogged filter, stress, or faulty sensor
• Remedy: check return line from sensor to return site, correct problem, slow down, dilute replacement solution, apply heat, calm patient/donor
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Apheresis Troubleshooting
Machine Components- Optical Sensors
Interface Detectors
Light absorbance
Camera
Line Sensors
Light absorbance or block
Cell effect on light-CCM red vs. green light
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Apheresis Troubleshooting Optical Sensor Troubleshooting
Proper positioning and loading
Avoid smudges, oils, and films
Keep clean-use recommended agents
Lipemic and hemolyzed plasma could be an issue
Watch for cracks, chips, and scratches
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Apheresis Troubleshooting Machine Components- Leak Detectors
Found in centrifuges
Detects current flow between electric wires
Usually humidity not an issue
Dust, particulate build-up could affect
Watch for tears and breaks after centrifuge line break
Use care cleaning
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Specific Problems
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Apheresis Troubleshooting Prime Failure
Test- Pump,Clamp,Sensor inTime
Checks:
Leak in kit
Pump performance
Clamp performance
Pressure sensor performance
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Apheresis Troubleshooting Prime Failure
Check:
Roller & pinch clamps completely closed
Clamps correctly loaded
Stacked tubing
Fluids completely open
Pumps loaded correctly
Pressure sensor has tight seal
Signs that pump roller may be sticky
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Apheresis Troubleshooting Air in line (post prime)
Possibilities:
Clot
Access site blocked-against wall of vessel or catheter
Kinked tubing before the pump head
Leak in tubing kit-connections, couplers, tubing
Leak in catheter
Clamped line
Replacement fluid not completely spiked
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Apheresis Troubleshooting Removing air in the line
Open lines and direct air to waste bag, saline bag, drip chamber
If in Return line:
Use a syringe at the injection site to draw into syringe
Disconnect from patient/donor, drain into waste container
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Apheresis Troubleshooting Hemolysis
Possibilities:
Frozen pump roller
Kink in tubing
Extended time in the centrifuge
Excessive heat in the centrifuge
Pre-existing hemolysis in patient
Wrong fluids attached
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Apheresis Troubleshooting Photo-Interface Creeps to Center of Bowl
Possibilities:
Bowl filled too fast, RBC not packed enough
Using port for access
Suggestion:
Drop collect rate to 20-25ml/min
If the interface stabilizes at the light beam, could raise the collect rate
If interface creeps, stay slow, decrease speed, put in pause
Change Double Needle to Single
Press STOP to re-establish interface
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Rippled Amicus Reflector
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Filtration Troubleshooting cont.
High TMP
• Causes: anticoagulation, plasma pump rate too high, high triglycerides
• Remedy: check anticoagulant, slow plasma pump, encourage better low fat diet for patient
High differential pressures
• Causes: clamped lines, kinks, return access anticoagulants, clogged separator, columns, or filter
• Remedy: correct obstructions, check anticoagulant
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Apheresis Troubleshooting
Best Practices in Trouble Shooting
Know your equipment
Check your equipment before you start
Use care during set-up and prime
Keep the equipment clean and in good shape
Do not panic; but reassure the donor/patient
Do localize, determine cause-effect, & correct
Do seek help, if needed
Inform vendors of problems with equipment and disposables