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TSETSE SURVEY IN SWAZILAND Report for WHO R.K. Saini & P.P. Simarro April to May 2008 Swaziland Collaborators: Ministry of Health and Social Welfare, Swaziland Ministry of Agriculture and Cooperatives, Swaziland Vestergaard Frandsen For further information, contact: Dr. Rajinder Kumar Saini icipe - Insect Science for Food and Health Email [email protected] Regional Centre for Mapping of Resources for Development
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Page 1: TSETSE SURVEY IN SWAZILAND

TSETSE SURVEY IN SWAZILAND Report for WHO

R.K. Saini & P.P. Simarro April to May 2008

Swaziland Collaborators:Ministry of Health and Social Welfare, Swaziland Ministry of Agriculture and Cooperatives, Swaziland Vestergaard Frandsen

For further information, contact: Dr. Rajinder Kumar Saini icipe - Insect Science for Food and Health Email [email protected]

Regional Centre for Mapping of Resources for Development

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TSETSE SURVEY IN SWAZILAND

Report for WHO

R.K. Saini & P.P. Simarro

The Principal Investigator of the Survey and WHO encourage fair use of this material provided

proper citation is made. No reproduction, copy or transmission of this report may be made

without permission of WHO and the PI.

Rajinder K Saini Pere P Simarro

Principal Investigator Human African Trypanosomasis icipe - Insect Science for Food and Health Innovative and Intensified Disease Management P.O.Box 30772, 00100 Control of Neglected Tropical Diseases Nairobi Communicable Diseases KENYA World Health Organization e-mail [email protected] 20 Avenue Appia, 1211 Geneva 27

Switzerland e-mail: [email protected]

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Contents

Executive Summary ...................................................................................................................... 5

Background to HAT and Tsetse Distribution in Swaziland ..................................................... 6

HAT cases in Swaziland.......................................................................................................... 6 Animal Trypanosomiasis (Nagana) cases in Swaziland ......................................................... 6 Distribution of Tsetse in Swaziland (from Historical & Current work in Southern Africa)... 7 Capacity for T&T Control in Swaziland ................................................................................. 8

Goal of the Project ........................................................................................................................ 8

Objectives: ..................................................................................................................................... 8

Sampling Methodology................................................................................................................. 8

a) Defining the Survey Area ................................................................................................ 8 b) Sampling Device and Trapping Methodology ................................................................ 9 c) Survey Teams ................................................................................................................ 10 d) Trap and Tsetse Distribution ........................................................................................ 10 e) Estimation of Apparent Density .................................................................................... 10 f) Sensitizing the Local Population to the Objectives of the Sampling Exercise.............. 10 g) Capacity Building ......................................................................................................... 11

Accomplishments of the Survey Exercise ................................................................................. 12

1. Area surveyed and insects caught................................................................................. 12 Vandalism of traps by Cows .................................................................................. 12 Tsetse flies trapped ................................................................................................ 13 Other biting flies trapped....................................................................................... 13 2. Community Sensitization............................................................................................... 13 3. Capacity Building ......................................................................................................... 14 4. Recommendations and Conclusions by the Survey Team ............................................. 14 5. Recommendations of Stakeholders ............................................................................... 15

Acknowledgments ....................................................................................................................... 17

Annexure...................................................................................................................................... 18

Annex 1 Swaziland Annual Medical and Sanitary Report 1928 ....................................... 19 Annex 2 Pre-Survey Questionnaire ................................................................................... 28 Annex 3a Distribution of tsetse in Swaziland...................................................................... 30

Fig. 1: Satellite image showing location of Swaziland................................................... 30 Fig. 2 Distribution of G. pallidipes in Zululand in relation to the occurrence of this species in Portuguese East Africa 31 Fig. 3 Historical distribution of tsetse in KwaZulu-Natal.............................................. 32 Fig. 4 Schematic drawing of historical fly distribution in South Africa and Mozambique.................................................................................................. 33Fig. 5 Historical fly distribution in South Africa and Mozambique .............................. 34 Fig. 6: Presence- absence prediction model indicating the probability of presence of G. austeni (A) and G. brevipalpis (B) in KwaZulu-Natal and Southern Mozambique ........................................................................................................ 35Fig. 7: Distribution of G. austeni (A) and G. brevipalpis (B) in KwaZulu-Natal .......... 36 Fig. 8 Prevalence of trypanosomosis in KwaZulu-Natal 37 Fig. 9: Predictive maps of Morsitans group of flies presence or absence in South Africa, Swaziland and Mozambique ................................................................................ 38 Fig. 10: Predictive maps of G. austeni presence or absence in South Africa, Swaziland and Mozambique.................................................................................................. 39

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Fig.11: Predictive maps of G. brevipalpis presence or absence in South Africa, Swaziland and Mozambique ............................................................................... 40 Fig. 12: Predictive maps of G. pallidipes presence or absence in South Africa, Swaziland and Mozambique ............................................................................... 41

Annex 3b Sources of Additional Information...................................................................... 42 Annex 4 Map of area Defined for Survey.......................................................................... 44 Annex 5 Coordinates of Centroids for Grids Surveyed..................................................... 45 Annex 6 Data Recording Sheet.......................................................................................... 47 Annex 7 Tsetse Survey Teams........................................................................................... 48 Annex 8a English Brochure for Community Sensitization.................................................. 51 Annex 8b siSwati Brochure for Community Sensitization.................................................. 53 Annex 8c Community Sensitization .................................................................................... 55 Annex 9 Schedule of Survey Work ................................................................................... 56 Annex 10 Typical day before and after field work .............................................................. 57 Annex 11 GIS MAPS: ......................................................................................................... 58

Map 1 Grid suitability and traps deployed ................................................................. 58 Map 2 Overview map of traps with tsetse flies.......................................................... 58 Map 3 Traps with tsetse flies...................................................................................... 58 Map 4 Traps with other biting flies (Stomoxys & Tabanids) .................................... 58

Annex 12 Habitats Surveyed .............................................................................................. 59 Annex 13 Trap Deployment (steps involved)..................................................................... 63 Annex 14 Traps Vandalized by cows ................................................................................. 66 Annex 15 Unaggregated Data Sheets ................................................................................. 67 Annex 16 Summary Data Sheet........................................................................................ 115 Annex 17 Gis Files and Maps Produced in the CD .......................................................... 117 Annex 18a) List of Participants for the Training of National Staff ................................... 118 Annex 18b) Capacity Building .......................................................................................... 120 Annex 19 Programme for Tsetse Debriefing Meeting .................................................... 121 Annex 20 List of Stakeholders Present in the Tsetse Debriefing Meeting ...................... 122 Annex 21 End of Survey Party hosted by Principal Investigator .................................... 124

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TSETSE SURVEY IN SWAZILAND

Executive Summary

A tsetse survey in Swaziland was undertaken in order to establish the true picture of trypanosomiasis in the country for it to be declared a Tsetse and Trypanosomiasis free zone, in view of the fact that no Human African Trypanosomiasis (HAT) cases have been reported for more than a century and only two African Animal Tryapanosomaisis cases have been reported in the last two decades (but even in these cases the animals came across the border). Based on historical fly distribution data, an area of approximately 7,475 km2 was originally demarcated for the survey. This area was divided into 75 grids (each 10 x 10 km). The actual area was further reduced to approximately 3,525 km2 on the basis of satellite images observed for each grid and field reconnaissance prior to deployment of traps. 372 H-traps were deployed in this area covering an area of approximately 3,000 km2. The survey area extended from Lavumisa in the South to Lomahasha in the North and the Northern part of Swaziland where Mbuluzi River starts right up to the area it enters Mozambique. This area falls in Hhohho, Lubombo and Shishelweni districts and a greater part of it falls in the lowveld area to the East, which borders Mozambique and South Africa. Glossina austeni in very low densities were found in Mlawula Park close to the Mozambique border. Even though the tsetse fly densities were very low this has implications for the creation of tsetse free zones in South Africa and in Southern part of Mozambique, as this area could be a site for reinvasion. Trap catches included >5,000 Stomoxys, >300 Tabanid flies and >2,000 other insects.

Prior to the survey exercise a brochure for community sensitization was prepared and translated into siSwati (local language). Nearly 4,500 copies of this brochure were distributed in the survey area to Farming Communities, Cattle Ranches, Royal Swazi Police, Royal Defense Army and Game Parks in order to sensitize the communities for the smooth operation of the survey exercise.

Capacity building was an integral part of the exercise and twenty National participants have been trained in basic tsetse survey methodologies and 7 participants who were derived from the relevant Ministries have got hands-on training in trap deployment, maintenance and data collection.

In view of the fact that the G. austeni is not involved in the transmission of HAT and no cases have been reported for nearly a century the labeling of Swaziland as an HAT endemic country is questionable. A second seasonal survey that should include a parasitological assessment is recommended to confirm the distribution of tsetse and determine the trypanosomes involved. The survey team and the stakeholders involved have made several other recommendations, which are highlighted, in the main report.

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Background to HAT and Tsetse Distribution in Swaziland

Tsetse flies, the vectors of sleeping sickness (Human African Trypanosomosis) and nagana (Animal African Trypanosomosis) are unique to Africa. The disease occurs in 36 sub-Saharan African countries covering close to 9 million km2, an area that corresponds approximately to one-third of the Africa’s total land area. Human African Trypanosomosis (HAT) threatens an estimated 60 million people while 45-50 million head of cattle are at risk of African Animal Trypanosomosis (AAT). In 1998, WHO estimated that about 300,000 to 500,000 HAT cases exist that largely remained undiagnosed and therefore, untreated. During recent epidemics in several villages in the Democratic Republic of Congo, Angola and Southern Sudan the disease prevalence reached up to 50% (WHO, 2006). Every year, AAT causes about 3 million deaths in cattle while approximately 35 million doses of trypanocidal drugs are administered. Nagana has a severe impact on agriculture and food security in sub-Saharan Africa. The economic losses in cattle production alone are in the range of US$ 1.0 – 1.2 billion. In a weighted evaluation Budd (1999) extrapolated for all tsetse-infested areas the total losses, in terms of agricultural Gross Domestic Products, at US$ 4.5 billion per year. African countries affected by trypanosomiasis recognise that the disease represents a major constraint to human and livestock health and better land use and to addressing the continuing problem of underdevelopment on the continent (Assembly of Heads of State and Government, July 2002, Lomé, Togo).

HAT cases in Swaziland

HAT is listed in WHO records to be endemic in Swaziland, yet for many years no HAT cases have been reported and consequently, WHO has not provided any drugs for treatment. During the initial planning mission in Swaziland (13th – 19th January 2008), the Statistics Department of the Ministry of Health in Swaziland indicated that they have no records of any HAT cases in Swaziland for last 10 years for which they have been keeping records. Medical reports from 1911 to 1934 available from the National Archives in Mbabane also have no documentation of any HAT cases or of the presence of vectors in the country. In fact, the Annual Medical and Sanitary Report of 1928 clearly states that there is no trypanosomiasis present in Swaziland. (See Annex 1, page 2)

Animal Trypanosomiasis (Nagana) cases in Swaziland

Prior to the planning mission in January, a Questionnaire (see Annex 2) was developed and circulated to the relevant Ministries and other Stakeholders in Swaziland in order to determine the knowledge and treatment of T & T and other animal diseases in the country. The answers received and in discussions with the staff of the Ministry of Livestock and Veterinary Services it was clear that trypanosomiasis was not a major problem in Swaziland and no cases of AAT have been reported in Swaziland for a long time. The Livestock and Veterinary Services Department mentioned that in 1980 and 2003, one animal was diagnosed with trypanosomiasis but in both cases the animal had came from KwaZulu Natal in South Africa. Tick and tick-borne diseases caused by a variety of ticks were the most prevalent animal diseases and in order to control them, farmers commonly used trypanocides. Dipping of cattle with acaricides was a common practice and mandatory for all livestock keepers. Acaricides were purchased by the government and provided at no cost to farmers. Knowledge about T & T was academic among the veterinary staff and completely lacking in farmers.

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After the survey was undertaken, the Principal investigator was informed by the Deputy Director of Department of Veterinary Services, that while going through the archives he had found in the Veterinary Services Annual Report of 1950 a mention about tsetse and trypanosomiasis in Swaziland when a special unit working on tsetse and trypanosomiasis within the Veterinary department existed. According to the report a few tsetse (G. austeni) were trapped in Mlawula Nature Reserve but it was concluded that the population was too small to cause any problem. Deforestation along the boarder of Mozambique was the main control strategy employed (the cordon line was supposed to be without thick vegetation). Trypanosoma congolense

was also diagnosed in a few animals in the Lavumisa area but all efforts to trap flies were unsuccessful. T. congolense has also been subsequently diagnosed in 2001 in the same area. Tsetse trapping in 1998 – 1990 undertaken by a Veterinarian from UK was unsuccessful.

Distribution of Tsetse in Swaziland (from Historical & Current work in Southern Africa)

No specific records exist of tsetse distribution in Swaziland per se but could be deduced from graphic and textual sources available for Southern Africa. Particularly relevant are the papers by Fuller (1923) and du Toit (1954) which provide guidelines for fly distributions up to the mid 50’s in Zululand and Transvaal respectively. In addition, the work of Kappemeier et al., (1998, 2007) and the AVIA-GIS (2002) report to IAEA provide important information on the distribution of flies in KwaZulu Natal and Southern Mozambique. Predictive maps (Annex 3a, Figs. 9-12) of tsetse presence or absence (http://ergodd.zoo.ox.ac.uk) also provide information on the limits of distribution of morsitans group of flies.

According to the work of du Toit (1954), Fuller (1923) and Kappemeier et al., (1998) (Annex

3a, Figs. 2 – 5) it may be concluded that the distribution of morsitans flies in two lobes extends from Southern Zimbabwe into North Eastern South Africa. Of, these two lobes, the Eastern one (Annex 3a, Figs. 4 & 5) extends into Northern Swaziland up to the Mbuluzi River (latitude 26° South; (suggestion of du Toit (1954). A further population identified as G. pallidipes by Kappemeier et al., (1998) and du Toit (1954) extends South East of Swaziland right into Kwa Zulu Natal. Thus, according to these authors G. pallidipes is also present in the South East corner of Swaziland. AVIA-GIS maps (Annex 3a, Figs 6 & 7), though not specific for Swaziland indicate absence of G. austeni and G. brevipalpis close to the South Eastern border of Swaziland. A study of the prevalence of trypanosomosis in Kwa-Zulu Natal (Annex 3a, Fig. 8) indicates very low incidence of the disease along the south eastern border of Swaziland (Kappemeier et

al., 1998). AAT is also prevalent in neighboring Mozambique. Interestingly, the strategy for the creation of a tsetse-free zone in the Republic of South Africa and in the Southern part of the Republic of Mozambique (Kappemeier et al., 2007) in order to achieve the objectives of the Pan African Tsetse and Trypanosomaisis Eradication Campaign (PATTEC), does not take into account the probability of the presence of flies in Swaziland neither along the border with South Africa or that of Mozambique. (see Annex 3b for Sources of Additional information)

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Capacity for T&T Control in Swaziland

Due to lack of recorded trypanosomiasis cases, there is also a lack of capacity in Swaziland with regards to vector control and survey methodologies.

In view of the above it was imperative to undertake an assessment on the presence or absence of the disease and the vector in the country and to establish the true picture of trypanosomiasis in Swaziland.

Goal of the Project

Establish the true picture of trypansomiasis in Swaziland for the country to be declared a Tsetse and Trypanosomaisis free zone.

Objectives:

The Primary objectives of the tsetse survey in Swaziland were:

Determine the precise presence, abundance and distribution of tsetse populations in the country

Determine all the tsetse species present in the area

Determine the relative abundance (apparent densities) of each species present and seasonal changes in apparent density in geo-referenced positions

Identify the main ecological niches (habitats) for each tsetse species

Determine the presence of other biting flies (Tabanidae, stomoxys spp.)

Assist PATTEC, South Africa and Mozambique to prepare appropriate plans for eradication of tsetse in the region

Capacitate national systems in tsetse trapping and survey methodologies

Circulate survey report to host country and all international stakeholders.

Sampling Methodology

a) Defining the Survey Area

The survey area was defined taking into consideration: all the historical and current information regarding tsetse distribution in Southern Africa

(as indicated above), using predictive tsetse maps (http://ergodd.zoo.ox.ac.uk), using satellite images (obtained from Regional Centre for Mapping of Resources for

Development (RCMRD) in Nairobi and National survey maps of Swaziland (obtained from the office of the Director General of

survey and mapping in Mbabane.

In view of the above, the survey area was defined as shown in Annex 4 and was divided in 10 x 10 km UTM grids for survey purposes. The survey area falls in Hhohho, Lumbombo and Shiselweni Districts. The Hhohho area comprises of the mountainous highveld with a temperate climate but was included as per the suggestion of Fuller (1923) and du Toit (1954). Greater part

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of the surveyed area falls in the Lowveld area to the East, which is sub-tropical and includes the Eastern zone along the Lubombo Mountains, which form Swaziland’s border with Mozambique. The survey area also covered parts of the Shiselweni region, Lubombo region and Hhohho region along the South African border. An identification system for each grid squared for assisting in the survey and in correctly assigning data was established. Initially 75 grids were demarcated for survey (an area of approximately 7,475 km2). Some of these grids were further subdivided into halves or quarters for surveying based on observations of satellite images of the respective grid and on land use maps.

In order to assist in the identification of grids and in navigation between the grids, the coordinates of the mid point of each grid (centroid) were worked out in advance and saved as way points on the GPS to be used in the field (see Annex 5)

b) Sampling Device and Trapping Methodology

H-traps (Kappmeier, 2000) provided free of charge by Vestergaard Frandsen were used as standard-sampling devices placed roughly 4/km2. Traps were placed in each grid depending on vegetation type, drainage system, accessibility and human activities (settlements or cultivated land). All traps were baited with cow urine dispensed at 100 mg/hr from plastic bottles with an aperture of 2x4 cm cut at the sides and with acetone, dispensed at 500mh/h from glass bottles with an aperture of 2mm at the top. All trap posts were greased to prevent ants from damaging the catch.

It was estimated that a minimum period of 3 days trapping per grid square of 10x10 km was required to reduce variability of trap catches to acceptable levels and was therefore taken as minimum period of sampling in each grid (determined to give 95% probability of detecting any tsetse in the area). On the fourth day after sampling, traps were removed and moved to the next set of grids.

In order to maximize on survey time, each team worked on a 5-day cycle as follows:

Day 1 Reconnaissance and deployment of traps in a grid (a 10 x 10 km grid) Day 2 Reconnaissance and deployment of traps in a second grid and sampled flies in grid 1. Day 3 Sampled flies in grids 1 & 2 Day 4 Sampled flies in grid 1 and removed traps, and sampled grid 2, reconnaissance

and deployment of traps in next set of grids. Day 5 Sampled flies in grid 2, removed traps and started deployment of traps in next grid.

The method of data collection and recording and the way it will be analyzed was standardized prior to the initiation of the survey. This involved having pre printed data recording sheets for field use (see Annex 6). The data recording sheets included detailed GIS coordinates of each trap deployed and the tsetse flies, biting or other insects caught. Each geo-referenced trap had a unique identifying number and a combination of letters indicating the grid in which it was placed, the trap number and the vegetation type.

For example: H5T06T indicates: Grid H5; Trap No. 06 and Vegetation as Thicket

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c) Survey Teams

Two survey teams each comprising of a technician driver, tsetse entomologist and one field technician were formed (see Annex 7 for details). These teams were responsible for reconnaissance, deployment of traps, collecting flies and initial recording of data. The third team comprised of a driver, the data entry person and the overall supervisor/manager of the survey. The latter team also assisted in deployment of traps and data collection. The field teams brought their data to the data entry person every evening, who downloaded and entered it into a single master database and also made backups of the data. The two teams were supervised by the Principal Investigator / Senior Tsetse Entomologist who ensured that the survey plan protocol was followed and also verified the accuracy of the data entry. GIS expertise was provided by a GIS expert from RCMRD who also developed the tsetse fly sample grids over the study area including scanning of maps and providing satellite imagery, plotting of GPS readings, development of tsetse spatial databases from field observations and production of maps.

Tsetse species were identified using conventional identification keys that are available in standard texts (e.g. from Mulligan, 1971; FAO training manual Vol.1 1982; or by using the computer identification software developed by IRD/CIRAD and available in CD-ROM.

d) Trap and Tsetse Distribution

All Grid-Squares and trap sites were geo-referenced using the Global Positioning System (Garmin GPS) for later incorporation in a Geographical Information System (GIS). The habitat types were classified according to the vegetation cover of the area e.g. woodland, grassland, cultivated land, forest and thicket. The coordinates of all trap sites were entered into a database and the information was used to produce tsetse and other biting fly’s distribution maps.

e) Estimation of Apparent Density

Since it is not possible to precisely estimate the density of a tsetse population in a given area, or even a rough estimate of the density by trapping, the apparent density (which roughly reflects changes in real density of the population) was calculated. The apparent density which is relative to the type of sampling tool (trap) used is expressed as the average number of flies caught per trap per day (flies/trap/day) or FTD. The apparent density was calculated by dividing the totalnumber of tsetse captured ( F) by the product of the number of functioning traps used to catch them (T) and the number of days for which traps were operational (D)

FTD = F/TxD

If a trap was not operational for some reason, e.g. it was vandalized, stolen, blown down or destroyed by cows, that trap-day was excluded from the sum of trap days.

f) Sensitizing the Local Population to the Objectives of the Sampling Exercise

Farming communities and the local authorities were sensitized on the survey to be undertaken and especially made familiar with the traps that were placed in their farms. Community involvement ensured that the technicians were allowed passage in their farms / ranches for deployment of traps and that the traps were protected and not vandalized. In order to sensitize the local communities, a brochure was prepared and translated into siSwati (see Annex 8a, b & c

for details). The officials from Ministry of Health and Social Welfare and Ministry of

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Agriculture and Cooperatives in Swaziland were given responsibility for sensitizing the communities and informing the Game Parks and Private Ranches in advance of the survey exercise.

g) Capacity Building

One of the major objectives of the survey was to capacitate the National staff in basic tsetse biology and ecology and undertaking baseline surveys. The following training courses were offered:

Basic tsetse biology and ecology Vector control techniques Baseline tsetse survey methodology Trap deployment and servicing and interpretation of trap catches Data collection

Since National staff were also part of the survey teams, hands-on-training in trapping of vectors was provided to them.

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Accomplishments of the Survey Exercise

The schedule of the entire exercise is shown in (Annex 9). The results of the survey are presented below under the following headings:

Area surveyed and insects caught Community sensitization Capacity building Recommendations and conclusions by the survey team Recommendations of stakeholders

1. Area surveyed and insects caught

A typical sampling day (Annex 10) was as follows:

0600h – 0630h Breakfast 0630h Departure for field 1800h Return from field 1900h – 2030h Dinner 2030h – 2115h Briefing of day’s activities and workplan for the next day

All the teams worked full time without any weekends from 12th April to 7th May 2008 (actual survey time)

The original area demarcated for the survey comprised of 75 grids (each 10 x 10 km) which is equivalent to approximately 7,475 km2

(Annex 4). This area was further reduced to approximately 3,525 km2 on the basis of satellite images observed for each grid and field reconnaissance prior to deployment of traps. This reduction was due to vegetation cover (man-made forests, cultivated lands), or human settlement (houses, villages, towns etc.). Annex 11,

Map 1 shows the grids suitable for sampling and the traps deployed in the various grids. The area in which 372 H-traps were deployed covered an area of 3,100 km2. This area extended from Lavumisa in the South to Lomahasha in the North and the Northern part of Swaziland including the area from where the Mbuluzi River starts right up to the area where it enters Mozambique. The area where Mbuluzi River starts was open grassland and the habitat is not suitable for tsetse. The various habitats surveyed including where traps were deployed are shown in Annex 12

while the entire exercise of a trap being deployed is shown in Annex 13. The survey teams were greatly assisted in trap deployment and protection along the Mozambique and South African borders by the soldiers of the Swaziland Royal Defense Army and by the Game wardens in the various games reserves surveyed.

Vandalism of traps by Cows

10 traps were completely destroyed by cows that partially ate the traps (see Annex 14). This peculiar behavior of the cows was seen along the Mozambique border in Grids J03 & J08. In fact, the soldiers of the Royal Defense Army of Swaziland warned us in advance that the cows in the region were notorious for eating cloth. Since community sensitization (see below) in advance was largely very successful, vandalism by people was minimum and only two traps were lost.

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Tsetse flies trapped

Annex 11, Maps 2 & 3 show the overall traps deployed and the traps in which tsetse were caught. The tsetse caught were identified as Glossina austeni. These flies were however caught in very low numbers (only 7 of which 3 were males and 4 were females). These tsetse flies were caught in Mlawula Nature Reserve (in Grid I05) close to the Mozambique border. No tsetse were caught in the neighbouring game reserves of Hlane and Mbuluzi. Similarly, no tsetse were caught in the Mbuluzi gorge in gird J04 which borders Mozambique. In fact, no flies were also caught along the Mbuluzi River in grids I04 and J04 up to the point it enters Mozambique. No tsetse were also caught along the South African border in the South, Southeast or North of Swaziland.

Other biting flies trapped

Annex 11, Map 4 shows the traps deployed and the traps in which other non tsetse biting flies were caught. While Stomoxys were wide spread in the areas surveyed (>5,000 Stomoxys caught), Tabanids were much fewer in numbers (only about 300 Tabanid flies were caught). Over 2300 other insects comprising of grasshoppers, moths, houseflies, wasps, bees, beetles etc were also caught in the traps.

Annex 15 contains all the Un-aggregated Data Sheets, while Annex 16 shows the Summary sheet of the traps deployed in various grids and the insects caught. Annex 17 in enclosed CD contains all the GIS files and the Maps produced.

2. Community Sensitization

The brochure prepared by Dr. R.K.Saini ‘Is Africa’s Deadly Fly (Tsetse) present in Swaziland was translated into siSwati (local language) by the Ministry of Agriculture and Cooperatives (see

Annex 8a, b & c). 4,400 copies of this brochure were distributed in the survey area to farming communities, cattle ranches, Royal Swazi Police, Royal Defense Army, and Game Parks etc. Members of the survey team explained the objectives of the survey to the various stakeholders prior to deployment of traps. On the whole, the survey teams received excellent collaboration and good will from the community members in all the areas surveyed.

All the Private Cattle Ranches and Game Parks which included: Hlane Royal National Park, Mlawula Nature Reserve, Mbuluzi Game Reserve and Nilsela Game Reserve were very cooperative and allowed free access to the game parks for the survey. They also provided game wardens to assist the survey teams and to protect them from wild animals. Mkhaya Game Reserve, however, was the exception and could not be surveyed because of the uncooperative attitude of the Management. One of the Royal Farms was also not accessible.

Community sensitization would have been much better, if the relevant Ministry had informed its extension services well in advance of the survey exercise. Some minor problems were encountered in Grid F15 near Salitje, Grid I03 near Lomahasha and Grid H07 near Kalangea (close to Siteki) where the community called the police due lack of information regarding the traps deployed within these sites. However, explanation by the members of the survey teams quickly resolved the misunderstanding and the tense situation.

In the entire survey exercise only 2 traps in Grids E01 and E02 were lost due to vandalism.

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3. Capacity Building

One of the objectives of the Trypanosomiasis survey in Swaziland was to capacitate the National Systems in tsetse trapping and survey methodologies so that in future indigenous capacity exists to undertake similar surveys. This objective was met through:

a training workshop held for 20 national participants and through hands-on field training of 7 participants.

Training of National Staff on Wednesday 9th

April at Siteki Hotel, Swaziland

20 participants attended the day long training workshop (see Annex 18 a & b). These participants included: 8 from the Ministry of Health and Social Welfare, 10 from the Ministry of Agriculture and Cooperatives and 2 from the WHO Regional Office. The workshop was opened by Dr. Roland Dlamini; Deputy Director, Veterinary Services.

Lectures were given on: Basic tsetse biology and ecology – Dr. R.K. Saini

Integrated vector control techniques – Dr. R.K. Saini

Baseline tsetse survey methodology – trap deployment, servicing, data collection and interpretation of data (John Andoke)

After the lectures a demonstration was given on how to deploy the H-trap (see Annex 18 b for

details)

Hands-on Training in Trap Deployment and Survey Methodologies

Seven participants – 3 each from the Ministries of Health and Agriculture and 1 participant from WHO, participated as survey team members for a whole month and hence have got hands-on training in trap deployment, maintenance and data collection. These 7 personnel can now be focal points for further training and undertaking any future surveys.

4. Recommendations and Conclusions by the Survey Team

Based on the above results the following conclusions can be drawn:

1. Swaziland is not a tsetse and trypanosomiasis free zone.

2. Since no human trypanosomiasis cases have been reported for more than a century and the vector present – G. austeni is not involved in transmission of human trypanosomiasis and in view of the fact that WHO has not provided any drugs for treatment of HAT cases in the last 10 decades, Swaziland cannot be labeled as a HAT endemic country. At the most it may be labeled as a country at risk.

3. Even though, only two cases of animal trypanosomiasis have been recorded in the last nearly 30 years, the presence of G. austeni, which is known to transmit nagana, cannot

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rule out Swaziland being at risk from animal trypanosomiasis especially along the Mozambique border where the situation should be monitored very carefully.

4. Creation of tsetse free zones in the Republic of South Africa and Southern part of Mozambique cannot ignore the presence of tsetse on the Eastern border of Swaziland with Mozambique. This area could be a site for reinvasion into Mozambique and hence in any control/eradication plans Swaziland must be included.

5. AU/PATTEC, PAAT and relevant authorities in South Africa and Mozambique should be made aware of the presence of G. austeni in Swaziland.

6. A second seasonal survey is recommended to WHO to confirm the further presence of tsetse in Swaziland. It is also recommended that this should be accompanied by a parasitological survey in order to determine the trypanosomes present in the flies and cattle (especially in the Mlawula Park area).

7. Mkhaya Game Reserve to which the survey teams were denied access needs to be surveyed. In addition, it is recommended that the deep valleys along the Mozambique border, need to be surveyed in more detail as G. austeni may be present there.

5. Recommendations of Stakeholders

The results of the survey were presented to all stakeholders at a debriefing meeting held at the Ministry of Agriculture and Cooperatives conference room on Friday 9th May, 2008. The agenda for the meeting is shown in (Annex 19) and the list of Stakeholders present is shown in (Annex

20). The Director of Veterinary Services, Dr. Thwala, chaired the meeting and the stakeholders present included the Permanent Secretaries from the Ministries of Agriculture and Health and representative of the WHO Country Office - Mbabane. The representatives of the Game Parks were also present.

The stakeholders thanked Dr. Saini for the professional manner in which the survey was undertaken and the results presented. They endorsed the recommendations and conclusions of the survey team and as the way forward recommended:

1. A request be made to WHO to support the second seasonal survey, which should include a parasitological analysis.

2. All partners including AU/PATTEC, PAAT and the neighbouring countries of Mozambique and South Africa be informed of the survey results.

3. Since flies were present in Swaziland, the Ministry of Agriculture and Cooperatives should undertake additional surveillance and monitor the nagana situation in the affected area.

4. Since no HAT cases have been reported for nearly a century in Swaziland the country requests WHO to remove it as being listed as an endemic country.

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5. The stakeholders agreed that after the second survey they would plan on elimination of the G. austeni pocket in Mlawula Nature Reserve or any other pockets along the Mozambique border.

6. The technical staff that had been trained and participated in the survey work could form the nucleus for survey of other vector borne diseases.

7. That community sensitization should be improved prior to any such survey and agreed to improve it prior to the second survey and also to get permission from Mkhaya Game Reserve and the King’s Farms for survey purposes.

8. That all the Swazi participants in the survey work be awarded certificates for their participation and for being trained in tsetse baseline survey methodologies.

9. That the final report be submitted in both hard copies and in CDs through the WHO Country Office.

Dr. Saini thanked them for their input and agreed to forward the above recommendations to WHO and other international bodies for their necessary action.

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Acknowledgments The Team Leader of the Trypanosomiasis survey mission in Swaziland, Dr. R. K. Saini would like to record his appreciation and gratitude to the following for their unreserved cooperation and assistance in ensuring that the survey was successfully carried out and completed on schedule (Annex 21).

1. WHO Headquarters, Geneva, Switzerland for providing the funds and their support.

2. WHO Country Office - Mbabane: Dr. E. Maganu – WR Dr. Augustine Ntilivaumda – HIV/AIDS Country Office Dr. Benjamin Gama - HIV/AIDS Country Office Mr. Ebrima Ndure – Administrative Officer Mrs. Hleli Dlamini – Administrative Assistant Mr. Thamsanqa Dlamini – ITC Officer for being the focal point of the survey and for all the administrative coordination and provision of one vehicle.

3. Ministry of Health and Social Welfare – Malaria Unit Dr. Simon Kunene – Malaria Programme Manager Zulisile Zulu – Malaria Researcher for providing two vehicles and 3 technical staff

4. Ministry of Agriculture and Cooperatives Dr. Robert Thwala – Director Dr. Roland Dlamini – Deputy Director for providing 3 technical staff and in community sensitization

5. Vestergaard Frandsen A/S – Mr. Torben Vestergaard Frandsen for free supply of tsetse H traps

6. Regional Centre for Mapping of Resources for Development (RCMRD), Nairobi, Kenya

7. Survey Team Members: John Andoke – ICIPE Nairobi Peter Muasa – ICIPE Nairobi Caroline Muya – ICIPE Nairobi David Camp – WHO Regional Office Vusie m’Kumalo – Veterinary Service, Manzini (MoAC) Samson Manana – Veterinary Service, Manzini (MoAC) Paoulos Khoza – Malaria Unit – (MoH) Joel Matse - Malaria Unit – (MoH) Musa Mndzeble - Malaria Unit – (MoH) Augustine Motsa - Veterinary Service, Manzini (MoAC) for their hard work

8. Royal Swazi Police and Royal Defense Force for providing security especially along the Mozambique and South African borders

9. Game Parks - Hlane Royal National Park; Nisela Park; Mlawula Nature Reserve; Mbuluzi Game Reserve; for providing game wardens and free access to the parks.

10. Cattle Ranches – Bar Circle Ranch; Crookers Farm; Iysis, King’s Farms and Mtfumunde Ranch for allowing free access to their private ranches for the survey.

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Annexure

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Annex 1 Swaziland Annual Medical and Sanitary Report for the year ended

31st December, 1928 (D. 11131/29.)

SWAZILAND.

ANNUAL MEDICAL AND SANITARY REPORT FOR THE YEAR ENDED

.31ST DECEMBER, 1928.

I. Administration.

(a) STAFF.

European.

1 Principal Medical Officer. 1 Medical Officer.3 Doctors (subsidized~. 3 Hospital Assistants and Dispensers. 1 Typist. 1 Female Nurse. 2 Female Nurses (subsidized).

Native.

4 Hospital Orderlies. 1 Laundress. 3 Native Employees.

One Hospital Assistant and Dispenser, 1 Native Orderly, and 1 Native Laundress were appointed during the year.

(h) LIST OF ORDINANCES AFFECTINO PUBLIC HEALTH ENACTED DURING THE YEAR

The Swaziland Medical Inspection of Schools Proclamation, No. 2 of 1928. The Regulations (High Commissioner’s Notice No. 12 of 1928) published under the above Proclamation.

(e) FINANCIAL.

The revenue earned by the Department was £56 13s. 3d. and the expenditure £5,104 Os. 7d. The ratio of medical expenditure to the total revenue of the Territory was as 1 to 14.

II. Public Health.

(a) GENERAL REMARKS.

(I) General Diseases.

A continually increasing knowledge of the nature of the prevalent diseases of the Territory leads to the following observations. Epilepsy is most extraordinarily prevalent all through the country. It is not possible to get at any figures, but the large number of out-patients who come to be treated for this complaint, the number of cases one finds in the goals and the number of offences directly traceable to the effects of this disease dealt with in the courts of the Assistant Commissioners point to a very high incidence of the malady. No feasible explanation of this has been put forward. Scurvy is always common towards the end of the winter and ‘disappears again in the early summer when a supply of green food becomes available.

Rheumatism is very prevalent in ‘the higher parts of, the country, the extreme and rapid variations in the climate and the higher rainfall in those parts of the country probably account for this. Diseases of the respiratory system are common in the parts of the country where rheumatism is prevalent, and the reasons are probably the same. Pneumonia is not common, but colds and chronic bronchitis occur~ frequently. Asthma is very common indeed in the country, and there must be something that tends to bring out this condition as persons from outside who have a tendency to it almost invariably get this disease after a few days spent in the Territory.

A few cases of pellagra crop up every year. Ainhum is common in the country. The experience of the Medical Officer, Hiatikulu, is that it is found mostly in males,

while most of my cases have occurred in females. Med. and San.—Gp. 6. 56/2179. 3. 3. 3. 24. 75. 9/29. (3840) M. & S.

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2

(II) Communicable Diseases.

Mosquito or insect-borne.

The only disease of this nature is malaria. This year there was a very severe outbreak all over the middle and low veld, and several cases of blackwater ‘fever occurred.

There is no trypanosomiasis, yellow fever, filariasis, or relapsing fever. One would expect the latter as the vector is common all over the country, is well known to the natives, and huts where it is known to exist are avoided by them. They consider the bite is poisonous, but no cases of relapsing fever have ever been found, the natives do not know the disease and the effects of the bite are considered by them to be immediate and only local.

Infectious Diseases.

Amoebic dysentery occurs as isolated cases all over the Territory and many more cases than usual were treated this year.

There was an outbreak of bacillary dysentery at Hluti in Hiatikulu District but prompt and effective measures were taken

and the outbreak soon stamped out. Typhoid. There was a serious outbreak of this at one of the European schools in the Hlatikulu area and one death

occurred. Here again prompt measures soon put an end to the outbreak. There were a few cases in Bremersdorp but not so many as usual. The installation of the filter bed in the water supply probably helped to diminish the cases. Unfortunately some parts of the township do not get their supply from this source. The water after filtration was bacteriologically tested andfound to be good.

The rest of the Territory was very free from this disease. I am afraid there can be no doubt that tuberculosis in all forms is slowly increasing amongst the natives. Bone and joint

affections are not very common but tuberculosis of the glands is quite common and pulmonary tuberculosis is also common, the latter is probably more common than hospital practice would tend to indicate as there is no doubt that many of these cases residing in distant kraals never seek assistance.

Syphilis does not seem to be on the increase. A gratifying feature in connection with this disease is that the number of infected natives who voluntarily seek treatment is on the increase every year. Statistics collected from the incidence of this disease amongst the natives working on the Gold Mines show that the Swazis are comparatively free from it.

There was no outbreak of any of the ordinary infectious fevers during the year.

Helminthic Diseases.

Taeniasis and ascariasis are very prevalent. Oxyuris vermicularis is found occasionally. Schistosomiasis is very common all over the country below a level of about 3,000 feet. Large numbers of natives are

treated for it. It i~ hard to control this disease in such a very scattered community but where townships exist in the infectedarea attempts are made to prevent the use of water known to he infected.

Ankylostomiasis, trichinosis and guinea worm are unknown.

Vital Statistics.

(1) General Native Population.

Estimated population in 1928, 120,000 (over-estimated in 1927). No other statistics are available. Registration is not compulsory, but every kraal should be compelled to report a death

and to give a short account of the nature of tile illness, so that some idea of the cause of death can be obtained.

(2) General European Population.

Estimated number of residents, 2,600. Total deaths, 20. Percentage of deaths to total residents, 0.76. No other statistics are available.

3

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(3) European Officials.

Table showing the Sick, Invaliding and Death-Rates of European Officials.

1926. 1927. 1928.

1.Total number of officials resident ... ... ... 81 83 85 2.Average number resident ... ... ... ... 76 80 80 3.Total number on Sick List ... ... ... ... 10 7 16 4.Total number of days on Sick List ... ... ... 440 201 617 5.Average daily number on Sick List ... ... ... 1.20 0.55 1.7 6.Percentage of sick to average number resident ... 1.58 0.68 2.12 7.Average number of days on Sick List for each patient 44 28.71 38.56 8.Average sick time to each resident ... ... ... 5.78 2.5 7.71 9.Total number invalided ,., .,, .., ... Nil Nil Nil 10.Percentage of invalidings to total residents ... ... Nil Nil Nil 11.Total deaths ... ... ... ... ... ... Nil Nil 2 12.Percentage of deaths to total residents ... ... Nil Nil 2.35 13.Percentage of deaths to average number resident ... Nil Nil 2.5 14.Number of cases of sickness contracted away from residence ... ... ... ... ... ... . Nil Nil Nil

(4) Native Officials.

Table showing the Sick, Invaliding and Death-Rates of Native Officials 1926. 1927. 1928.

1.Total number of officials resident ... ... ... 147 144 145 ‘2.Average number resident ... ... ... ... 137 135 135 3.Total number on Sick List ... ... ... ... 62 49 55 4.Total number of days on Sick List ... ... 634 262 650 5.Average daily number on Sick List ... ... ... 1.73 0.71 1.78 o.Percentage of sick to average number resident ... 1.26 0.49 1.31 7.Average number of days on Sick List for each patient 10.22 5.34 11.81 8.Average sick time to each resident ... ... ... 4.62 1.9 4.81 9.Total number invalided ... ... ... ... 1 Nil Nil 10.Percentage of invalidings to total residents ... ... 0,68 Nil Nil 11.Total deaths ... ... ... ... ... ... Nil Nil Nil 12.Percentage of deaths to total residents ... ... Nil Nil Nil 13.Percentage of deaths to average, number resident ... Nil Nil Nil 14.Number of cases of sickness contracted away from residence ... ... ... ... ... ... 3 1 2

III. Hygiene and Sanitation.

There is no Sanitary Department.

(A) General review of work done and progress made.

1. Preventive Measures.

Mosquito and Insect-Borne Diseases. Distribution of quinine was continued as in past years.

Epidemic Diseases. Typhoid vaccine is administered as required.

Helminthic Diseases.— The position continues the same as mentioned in previous reports.

II. General Measures of Sanitation.

Government-controlled bucket latrines are used in township areas, and persons outside these areas have to conform to this system, which is the best practical system under present conditions.

The water supply, usually obtained from springs, is fairly good. Sanitary inspections are made from time to time.

.111. School Hygiene.

This is directed by the teachers.

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4

1V. Labour Conditions.

Nearly all the natives working in the country are employed as farm labourers.. Practically the only industries are the tin mines, these are alluvial and therefore all surface work, and a small gold mine at Forbes Reef which is only in the development stage and employs only a few natives. Measures are being taken to improve the sanitary conditions on the tin mines.

V. Housing and Town Planning.

There is nothing to report under ‘this head.

VI. Food in relation to Health and Disease,

Food supplies’ have not given any trouble, and, under existing conditions, regular inspection is not necessary. Slaughtering is done at specially appointed places, and is supervised by the Police. During most seasons of the year the natives have a plentiful supply of food, although they suffer from a lack of green food during the winter months.

B. Measures taken to spread the knowledge of Hygiene and Sanitation.

None, except that incorporated in school teaching.

D. Recommendations for future work.

Measures should be taken to improve the water supply in all townships.

IV. Port Health Work and Administration.

Not applicable. V. Maternity and Child Welfare.

There is nothing to report under this head.

VI. Hospitals, Dispensaries, and Venereal Clinics.

As it is almost certain that the new Mbabane Hospital will be built next year,. only a brief reference will be made to the present Hospital, which is obsolete.

254 Patients were’ admitted during the year, which with the 31 remaining from the previous year gives a total of 286 treated in Hospital.

There were 8 deaths. Chief causes of admission were syphilis, malaria and injuries. 7,050 out-patients were treated during the year. The new Government Hospital at Hiatikulu was opened for out-patients in January,. and for in-patients on 30th

October. It is small but excellently equipped, and has accommodation for 4 European and. 4 Native patients. It is hoped that it will be found possible to enlarge this hospital in the near future,. as it is much too small.

The staff consists of 1 European Hospital Assistant in charge, 1 female European trained nurse, 1 Native laundress and

nurse help, and 2 Native orderlies. 12 cases were admitted. There were no deaths. 3,409 out-patients were treated during the year. The Mission Hospitals at Bremersdorp and Mahamba continue to do very useful’ work, and are treating increasing

numbers of patients. They are both excellently equipped and staffed. Available details appear in the annexure to Table V. The Government Dispensary at Mankaiana, and Mission Dispensaries at .Stegi and Pigg’s Peak are also doing useful

work. .

TABLE I.

Medical Staff.

Dr. B. Jamison, Principal Medical Officer; Dr. F. A. Donolly, Medical Officer.

Subsidized Doctors. .

Dr. C. S. Gibbons, Mbahane; Dr. B. Hynd, Bremersdorp; Dr. A. T. Till, Mahamba.

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5

Principal Members of the Subordinate Staff.

Mr. II. B. Barnard, Hospital Assistant; Mr. J. O’N. Anderson, Hospital Assistant; Mr. A. G. Lunnis, Hospital Assistant; Mrs. Rose, Nurse; Mrs. M. L. Anderson, Typist.

Subsidized.

Miss Pelley, Nurse; Miss Carpenter, Nurse.

Principal Changes.

Mr. A. 0. Lunnis was appointed during the year.

TABLE II.

Revenue of Swaziland ... ... £114,301. 13 5 Expenditure .of Swaziland ... £123,930 6 6

TABLE III.

This table cannot be compiled.

TABLE IV.

Meteorological Return for the Year 1928.

Station—Mbabane. Temperature. Rainfall.

Max. Mm. Mean. Inches.

January ... ... 91 53 72 8.35 February ... ... 92 48 ‘ 70 2.34

March ... ... 88 50 69 3.73

April ... ... 84 43 63 2.85

May ... ... 74 38 56 0.86 June ... ... 74 32 53 Nil July ... ... 75 33 54 0.25 August ... ... 87 36 6]. 1.15 September ... ... 90 40 65 2.23

October ... ... 98 44 71 3.93 November ... ... 96 47 71 6.03

December ... ... 92 50 71 7.96

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TABLE V.

Hospital or Institution :—Government Hospital, Mbabane.

Return of Diseases and Deaths (In-patients) for the Year 1928. Remaining Yearly Total. TotalRemaining

in Hospital . in Hospital

Drsease9. at end of Deaths. at end of 1927. 1928.

I.Epidemic, Endemic and In-

fectious Diseases.

5.Malaria (a) Tertian ... 1 40 1 41 5 16.Dysentery (a) Amoebic ... 1 1 25.(b) Varicella ... ... 1 1 31.Tuberculosis, Pulmonary 1 1 1 35.Tuberculosis of Bones and Joints ... ... 1 2 3 2 36.Tuberculosis of other organs (c) Lymphatic system ... ... 1 ‘ 1 38.Syphilis (b) Secondary ... 13 65 68 6 39.Soft Chancre ,.. ... 2 2 40.A. Gonorrhea and its complications ... 5 5 B. Gonorrheal Ophthalmia 2 2

Total carried forward 18 107 2 125 14

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TABLE V.—continued. Remaining Yearly Total. Total Remaining

Diseases in Hospital Admis- Deaths. cases in Hospital

1927. sions Treated. 1928. Brought forward ... 1.8 107 2 125 14

II. General Diseases not mentioned above.

48.Cancer or other malig nant tumours of the skin 1 1 50.Tumours non-malignant 3 3 51.Acute Rheunmatism ... 1 10 11 53.Scurvy ... ... 1 1 2 64.Pellagra ... ... 1 1 58.(b) Other Anaemias ... 1 1 69. Other General Diseases... 4 1 4 III.Affections of the Nervous

System and Organs of the

Senses.

74.Apoplexy (a) Haemorr

hage... ... ... 2 2 2

77.Other forms of Mental’

Alienation ... ... 1 1 1 78.Epilepsy ... ... 2 2 82.B. Neuritis ... ... 1 1

85.Affections. of the Organs

of Vision. (a)Diseases of the Eye 5 5 2 (e)Other Affections of the Eye ... ... 1 1 86.Affections of the Ear ... 1 1 IV. Affections of the Circulatory System.

92. Thrombosis ... ... 2 2

93. Diseases of the Veins.

Haemorrhoids ... 2 2 Phlebitis ... ... 2 2

V. Affections of the Respiratory System.97. Rhinitis ... ... 1 1

98. Bronchitis (a)Acute ... 3 3 101. Pneumonia (a)Lobar ... 2 1 2 102. Pleurisy... ... ... 1 1 105. Asthma ... ... ... 2 2

VI. Diseases of tile Digestive

System.

111. A. Ulcer of the Stomach 1 1 112. Gastritis... ... ... 1 1 114. Diarrhoea and Enteritis. Two years and over... 2 2 Colitis ... ... 1 1 117. Appendicitis ... ... 2 2

127. Other affections of the

Digestive System ... 1 1 VII. Diseases of the Genito- urinary System (non-

‘Venereal).

128. Acute Nephritis ... 1 1 1.30. B. Schistosorniasis ... 4 4 Total carried forward 21 168 7 189 19

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7TABLE V.’—continued.

Remaining Yearly Total. Total Remaining

in Hospital . in Hospital

Diseases. at end of Admis- Deaths. Cases at end of

1927. smons. Treated. 1928.

Brought forward ... 21 168 7 189 19 136. Diseases (non-Venereal) of the. Genital Organs of Man ... ... 3 . 3Orchitis ... ... ... ‘ 3 3 Hydrocele ... ... 1 1 141. A. Metritis ... ... 11 11 B. Other affections of the Female Genital Organs ... ... 1 1 Displacements of Uterus 1 1

IX.. Affections of the Skin and Cellular Tissues.

153. Abscess ... ... 2 2

Oellulitis ... ... 7 7

154. B. Scabies ... ... 3 3 155. Other Diseasesof the Skin 1 3 4

X.. Diseases of Bones and Organs of Loco-

motion (other than Tuberculosis)

157. Synovitis ... ... 1 1 1.58. Other Diseases of Bones or Organs of Locomotion ... 5 5 2 XI. Malformations.

159. Malformations ... 1 1 XIV. Affections produced by

External Cause’s.

176. Snake Bite ... ... 2 2 178. Burns (by Fire) ... 7 1 7 1 183. Wounds (by Firearms, War excepted). ... 1 1 184. Wounds (by cutting or Stabbing instruments)... 11 U 1.88. Wounds (crushing) ... 2 2 201. B. Sprain ... ... ‘ 2 2 C. Fracture ... ... 4 9 13 202. Other External Injuries... 4 9 13 XV. Ill-defined Diseases.

205. A. Ascites: ... 2 2 Total ... 31 254 8 285 23

TABLE VI.

The number of Out-Patients treated was 7,050.

Government Hospital, Hlatikulu.

Admitted (30th October to 31st December), 12. Deaths, Nil. Out-Patients treated (full year), 3,409.

Government Dispensary, Mankaiana.

Staff: 1 European Hospital Assistant. Out-Patients treated (18th September to 31st December), 477.

No records available prior to 18th September.

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Annex 2 Pre-Survey Questionnaire

1. Which are the most prevalent animal diseases in Swaziland?

2. How are these diseases treated?

3. Do farmers treat animals themselves?

4. Which veterinary drugs, are commonly used?

5. Are paravets common in Swaziland?

6. Do farmers/veterinarians/shops use or stock trypanocides?

7. Do livestock keepers know symptoms of nagana?

8. Have livestock owners ever reported any strange disease?

9. How efficient is dipping in the country and which acaricides are being used?

10. Are any pour-on insecticides commonly used on cattle?

11. When was the last trypanosomiasis survey undertaken?

12. Are records available of past trypanosomiasis surveys?

13. Are any records of Human Trypanosomiasis cases in Swaziland available? (These may be

published records or Ministry of Health Records).

14. When were cases of nagana last reported? Are records available?

15. Which tsetse species have been reported to be present in Swaziland? Are any

distributions maps available?

16. Is there trans border cattle grazing in the South Eastern border of South Africa (Kwazulu

Natal) /Mozambique?

17. Is there cattle trade /movement to neighboring countries or from neighboring countries

into Swaziland? If so, is there any screening of the animals undertaken?

18. Is there inter border movement of wild hosts of trypanosomosis between neighboring

countries?

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19. In case of tsetse infestation which areas would be the most suspected?

20. What would be the best time for any seasonal surveys to be undertaken?

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Page 38: TSETSE SURVEY IN SWAZILAND

38

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Page 39: TSETSE SURVEY IN SWAZILAND

39

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0:

P

red

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ve

ma

ps

of

G. a

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pre

sen

ce o

r a

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nce

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Page 40: TSETSE SURVEY IN SWAZILAND

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ps

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Page 41: TSETSE SURVEY IN SWAZILAND

41

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. 1

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42

Annex 3b Sources of Additional Information

(AVIA-GIS) Agriculture and Veterinary Intelligence and Analysis. 2002. Tsetse presence- absence prediction model for Glossina austeni and Glossina brevipalpis in KwaZulu- Natal – South Africa. Report to the International Energy Atomic Agency. IAEA, Vienna Austria.

Biesemans, J., Hendrickx, G., Van Camp, N., Kappmeier, K., Williams, R. & Nevill, E. 2002. Geostatistical Space-Time Population Dynamics Modeling of Glossina spp. in Kwa Zulu-Natal: Proceedings of the SVEPM Annual Conference 2003

Du Toit, R. 1954. Trypanosomiasis in Zululand and the control of tsetse flies by chemical means. Onderstepoort Journal of Veterinary Research 26: 317-387.

Du Toit, R. 1959. ‘Trypanosomiasis in Zululand’ and ‘The Eradication of the Tsetse Fly (Glossina pallidipes) from Zululand, Union of South Africa’, in C. Brandly and E.L. Jungherr (eds), Advances in Veterinary Science, Volume 5 (New York, Academic Press, 1959), pp. 227–40.

Fuller, C. 1923. Tsetse in the Transvaal and Surrounding Territories – An Historical Review. Entomology Memoirs, Memoir No 1, Division of Entomology, Pretoria 1923, Department of Agriculture, Union of South Africa. (Also available as Ninth and Tenth Reports of the Director of Veterinary Education and Research, Union of South Africa, and in Book format).

Hendrickx, G., Nevill, E., Biesemans, J., Kappmeier-Green, K., Van Camp, N. and Williams, R. (2002) The Use of Geostatistics and Remote Sensing to Optimize Tsetse Field Survey Results. The example of KwaZulu-Natal (RSA). Newsletter on Integrated Control of Pathogenic Trypanosomes and their Vectors

Kappemeier K., Nevill, E. M. and Bagnall, R. J. 1998. Review of tsetse and trypanosomosis in South Africa. Onderstepoort Journal of Veterinary Research 65: 195-203.

Kappmeier, K. (2000) A newly developed baited-baited “H trap” for the live collection of Glossina brevipalpis and Glossina austeni (Diptera: Glossinidae) in South Africa.

Onderstepoort Journal of Veterinary Research, 67: 15-26.

Kappmeier Green, K. 2002. Strategy for monitoring and sustainable integrated control or eradication of Glossina brevipalpis and Glossina austeni (Diptera: Glossinidae) in South Africa. Ph.D Dissertation. University of Pretoria, Pretoria, South Africa.

Kappemeier, Green, K. 2003. A proposed strategy for tsetse control in KwaZulu-Natal. Newsletter on Integrated Control of Pathogenic Trypanosomes and their Vectors (ICPTV) 7: 30-33

Kappmeier Green, K., Potgieter, F. T. and Verysen, M. J. B. 2007. A strategy for an area- wide control campaign with an SIT component to establish a tsetse-free South Africa. In Vreysen, M. J. B., Robinson, A. S. and Hendrichs, J. (Eds), Area-Wide Control of

Page 43: TSETSE SURVEY IN SWAZILAND

43

Insect Pests: From Research to Field Implementation. Springer, Dordrecht, The Netherlands.

Knight, J. D. 2006. An examination of the costs and benefits of tsetse control in KwaZulu- Natal including the provision of a public relations campaign. Report to the joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. IAEA Vienna, Austria.

Parker, G. 2003. Situation analysis of the feasibility and desirability for tsetse fly eradication, KwaZulu-Natal, South Africa. Report to the International Energy Atomic Agency. IAEA, Vienna Austria.

Sigauque, I., Van Den Bossche, P., Moiana, M., Jamal, S. & Neves, L. (2000) The distribution of tsetse (Diptera: Glossinidae) and bovine trypanosomosis in the Matutuine District, Maputo Province, Mozambique. Onderstepoort Journal of Veterinary Research, 67: 167-172.

Van den Bossche, P., Esterhuizen, J., Nkuna, R., Matjila, T., Penzhorn, B. L., Geerts, S. and Marchotty, T. 2006. An update of the bovine trypanosomosis situation at the edge of the Hluhluwe-iMfolozi Park, KwaZulu-Natal Province, South Africa. Onderstepoort Journal of Veterinary Research 73: 77-79.

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44

Annex 4 Map of area Defined for Survey

Page 45: TSETSE SURVEY IN SWAZILAND

45

Annex 5 Coordinates of Centroids for Grids Surveyed

Alphanumeric Code

NumericCode

GridCode Latitude (Y) Longitude (X) For Sampling

A 00 A00 -25.76292 31.17927 NO A 01 A01 -25.86292 31.17927 NO A 02 A02 -25.96292 31.17927 NO A 03 A03 -26.06292 31.17927 YES B 00 B00 -25.76292 31.27927 YES B 01 B01 -25.86292 31.27927 YES B 02 B02 -25.96292 31.27927 NO B 03 B03 -26.06292 31.27927 YES C 00 C00 -25.76292 31.37927 YES C 01 C01 -25.86292 31.37927 YES C 02 C02 -25.96292 31.37927 NO C 03 C03 -26.06292 31.37927 YES D 02 D02 -25.96292 31.47927 YES D 00 D00 -25.76292 31.47927 YES D 01 D01 -25.86292 31.47927 YES D 03 D03 -26.06292 31.47927 YES E 02 E02 -25.96292 31.57927 YES E 00 E00 -25.76292 31.57927 YES E 01 E01 -25.86292 31.57927 YES E 03 E03 -26.06292 31.57927 YES F 05 F05 -26.26292 31.67927 NO F 06 F06 -26.36292 31.67927 NO F 07 F07 -26.46292 31.67927 NO F 08 F08 -26.56292 31.67927 YES F 09 F09 -26.66292 31.67927 NO F 10 F10 -26.76292 31.67927 NO F 11 F11 -26.86292 31.67927 NO F 12 F12 -26.96292 31.67927 NO F 13 F13 -27.06292 31.67927 NO F 14 F14 -27.16292 31.67927 NO F 15 F15 -27.26292 31.67927 YES F 04 F04 -26.16292 31.67927 NO F 02 F02 -25.96292 31.67927 NO F 03 F03 -26.06292 31.67927 YES F 01 F01 -25.86292 31.67927 NO G 04 G04 -26.16292 31.77927 NO G 05 G05 -26.26292 31.77927 YES G 06 G06 -26.36292 31.77927 YES G 07 G07 -26.46292 31.77927 NO G 08 G08 -26.56292 31.77927 YES G 09 G09 -26.66292 31.77927 YES G 10 G10 -26.76292 31.77927 YES G 11 G11 -26.86292 31.77927 YES G 12 G12 -26.96292 31.77927 NO G 13 G13 -27.06292 31.77927 YES

Page 46: TSETSE SURVEY IN SWAZILAND

46

G 14 G14 -27.16292 31.77927 YES G 15 G15 -27.26292 31.77927 YES G 02 G02 -25.96292 31.77927 NO G 03 G03 -26.06292 31.77927 YES H 02 H02 -25.96292 31.87927 NO H 03 H03 -26.06292 31.87927 NO H 04 H04 -26.16292 31.87927 YES H 05 H05 -26.26292 31.87927 YES H 06 H06 -26.36292 31.87927 YES H 07 H07 -26.46292 31.87927 YES H 08 H08 -26.56292 31.87927 NO H 09 H09 -26.66292 31.87927 YES H 10 H10 -26.76292 31.87927 YES H 11 H11 -26.86292 31.87927 YES H 12 H12 -26.96292 31.87927 YES H 13 H13 -27.06292 31.87927 NO H 14 H14 -27.16292 31.87927 YES H 15 H15 -27.26292 31.87927 YES I 02 I02 -25.96292 31.97927 YES I 03 I03 -26.06292 31.97927 YES I 04 I04 -26.16292 31.97927 YES I 05 I05 -26.26292 31.97927 YES I 06 I06 -26.36292 31.97927 YES I 07 I07 -26.46292 31.97927 NO I 08 I08 -26.56292 31.97927 YES I 09 I09 -26.66292 31.97927 NO I 10 I10 -26.76292 31.97927 NO I 11 I11 -26.86292 31.97927 YES I 12 I12 -26.96292 31.97927 YES I 13 I13 -27.06292 31.97927 YES I 14 I14 -27.16292 31.97927 YES I 15 I15 -27.26292 31.97927 YES J 02 J02 -25.96292 32.07927 YES J 03 J03 -26.06292 32.07927 YES J 04 J04 -26.16292 32.07927 YES J 05 J05 -26.26292 32.07927 YES J 06 J06 -26.36292 32.07927 YES J 07 J07 -26.46292 32.07927 YES J 08 JO8 -26.56292 32.07927 YES J 09 J09 -26.66292 32.07927 YES J 10 J10 -26.76292 32.07927 YES J 11 J11 -26.86292 32.07927 NO

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47

An

nex

6

Da

ta R

eco

rdin

g S

hee

t

Pa

ge:

_

__

__

C

ou

ntr

y S

wa

zila

nd

Gri

d I

D:

____

____

____

____

____

_

UT

M Z

ON

E:

____________________

__

__

D

ate

: _

__

__

__

__

__

__

__

__

__

__

Tea

m N

o:

_____

____

____

____

____

Da

ta o

ffic

er:

_____

____

____

____

____

___

Sh

eet

No

: __

____

____

____

___

Tra

p N

o

Lat

itude

(y)

coord

inat

e

Longit

ude

(x)

coord

inat

e

Alt

itude

Veg

. T

ype

Tse

tse

Bit

ing F

lies

O

ther

s R

emar

ks

Spec

ies

Mal

es

Fem

ales

T

ota

l F

amil

y

N

o.

Page 48: TSETSE SURVEY IN SWAZILAND

48

Annex 7 Tsetse Survey Teams

Vusie m’ Khumalo Animal Health Inspector Ministry of Agriculture & Cooperatives Manzini, Swaziland

Samson MananaAnimal Health Inspector Ministry of Agriculture & Cooperatives Siteki, Swaziland

Paulos M. Khoza

Health Assistant, Malaria Health Unit Ministry of Health (MOH) Manzini, Swaziland

John Andoke Akiri

Tsetse Entomologist ICIPE Nairobi, Kenya

Peter Nthale MuasaTsetse Entomologist ICIPE Nairobi, Kenya

Musa Mndzebele

Health Assistant, Malaria Health Unit Ministry of Health (MOH) Manzini, Swaziland

TEAM: 1

TEAM: 2

Page 49: TSETSE SURVEY IN SWAZILAND

49

Augustine MotsaVeterinary Assistant Ministry of Agriculture & Cooperatives Manzini, Swaziland

Joel M. Matse Health Assistant, Malaria Health Unit Ministry of Health (MOH) Manzini, Swaziland

Caroline Muthoni Muya

Data Manager ICIPE Nairobi, Kenya

David Camp

Transport Assistant World Health Organization (WHO) Mbabane, Swaziland

Group photo of the survey team

Dr. Rajinder K. Saini

Principal Investigator ICIPE Nairobi, Kenya

TEAM 3 - MANAGEMENT TEAM

Page 50: TSETSE SURVEY IN SWAZILAND

50

Contacts of Survey Team Members

NAME AFFILIATION CONTACT ADDRESS

MANAGEMENT TEAM Dr. Rajinder K. Saini Principal Investigator

ICIPE Nairobi Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya [email protected]

Caroline Muthoni Muya Data Manager ICIPE Nairobi

Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

David Camp Transport Assistant WHO

WHO Cell: +268 6237447 P.O.Box 903, Mbabane, Swaziland

TEAM 1 John Andoke Akiri Tsetse Entomologist

ICIPE Nairobi Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

Samson Manana Animal Health Inspector MOAC

Cell: +268 6081971 P.O.Box 43, Siteki, Swaziland

Vusie m’ Khumalo Animal Health Inspector MOAC

Cell: +268 6130720; Fax: +268 5056443 P.O.Box 4192, Manzini, Swaziland

Paulos M. Khoza Ministry of Health (MOH) Health Assistant Unit Malaria Health

Cell: +268 6079372 P.O.Box 53, Manzini, Swaziland

TEAM 2 Peter Nthale Muasa Tsetse Entomologist

ICIPE Nairobi Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

Augustine Motsa Veterinary Assistant MOAC

Cell: +268 6242040 P.O.Box 43, Siteki, Swaziland

Joel M. Matse Health Assistant Malaria Health Unit MOH

Cell: +268 6082155 P.O.Box 53, Manzini, Swaziland

Musa Mndzebele Health Assistant Malaria Health Unit MOH

Cell: +268 6352601 P.O.Box 53, Manzini, Swaziland

Page 51: TSETSE SURVEY IN SWAZILAND

51

An

nex

8a

En

gli

sh B

roch

ure

fo

r C

om

mu

nit

y S

ensi

tiza

tio

n

How

can

you

an

d t

he

farm

ing

co

mm

un

ity

hel

p?

Coo

per

atio

n o

f th

e fa

rmer

s is

ver

y i

mp

ort

ant

for

su

cces

sfu

l su

rvey

s.

Mo

reo

ver

, th

e in

form

atio

n

wil

l ben

efit

th

e fa

rmin

g c

om

mu

nit

y d

irec

tly

as

th

ere

anim

als

are

at r

isk

. In

ord

er t

o h

elp

ple

ase:

A

llo

w t

he

tech

nic

ian

s to

dep

loy

the

trap

s

in y

our

farm

s o

r al

low

pas

sage

thro

ugh

th

em

P

rote

ct t

he

trap

s an

d k

eep

ch

ildre

n a

way

fr

om

th

em.

ST

OP

any

one

fro

m S

TE

AL

ING

th

em

A

lso p

rote

ct t

he

bott

les

wit

h t

he

attr

acti

ve

sub

stan

ces

A

void

touch

ing t

he

chem

ical

su

bst

ance

s,

as

they

may

be

tox

ic a

nd

fla

mm

able

A

vo

id f

ires

in

are

as w

her

e tr

aps

are

dep

loy

ed

B

e as

sure

d t

hat

the

trap

s ar

e n

ot

har

mfu

l to

the

env

iro

nm

ent,

p

eop

le o

r th

eir

anim

als

A

ssis

t th

e su

rvey

tea

ms

in t

hei

r w

ork

Is A

fric

a’s

Dea

dly

Fly

(T

sets

e)

pre

sen

t in

Sw

azi

lan

d?

A s

urv

ey t

o b

e u

nd

erta

ken

by

:

M

inis

try

of

Hea

lth

an

d S

oci

al W

elfa

re,

Sw

azil

and

Min

istr

y o

f A

gri

cult

ure

an

d C

oo

per

ativ

es,

Sw

azil

and

ICIP

E,

Nai

rob

i, K

eny

a

Wit

h t

he

sup

po

rt o

f:W

HO

Pre

par

ed b

y:

Dr.

Ra

jin

der

K.

Sain

i

Inte

rnat

ional

Cen

tre

of

Inse

ct P

hy

sio

log

y a

nd

Eco

logy

(IC

IPE

) P

.O.B

ox

307

72

– 0

010

0

Nai

robi,

Ken

ya

Page 52: TSETSE SURVEY IN SWAZILAND

52

Wh

at

is a

Tse

tse

Fly

?

It i

s a

dea

dly

blo

od

fee

din

g, d

isea

se-c

arry

ing

fly

, al

mo

st t

he

size

of

a h

ou

sefl

y.

It d

efer

s fr

om

oth

er b

itin

g f

lies

by

th

e fa

ct t

hat

at

rest

its

win

gs

are

cro

ssed

ov

er i

ts a

bdo

men

lik

e a

scis

sor

wit

h i

ts p

robo

scis

po

inte

d

ou

t. B

oth

mal

e an

d f

emal

e fl

ies

feed

on b

loo

d.

Th

e m

ou

thpar

ts a

re

tail

ore

d f

or

bit

ing a

nd

su

ckin

g.

Fli

es a

re f

ou

nd

in 3

2 A

fric

an c

ou

ntr

ies.

T

he

word

tse

tse

com

es f

rom

Mat

abél

é o

r Z

ulu

peo

ple

.

Wh

y W

orr

y A

bou

t T

sets

e F

lies

?

Fli

es a

re k

no

wn t

o t

ransm

it a

dea

dly

par

asit

e ca

lled

a t

rypan

oso

me

fro

m

wil

d a

nim

als

(bu

ffal

os,

bu

sh p

igs,

war

tho

gs

etc)

to

hu

man

bei

ng

s an

d

do

mes

tic

anim

als

(cat

tle)

. T

his

par

asit

e d

oes

no

t ca

use

dis

ease

in

wil

d

anim

als

bu

t is

fat

al t

o b

oth

hu

man

s (h

um

an s

leep

ing s

icknes

s) a

nd c

attl

e (n

agan

a). N

agan

a m

ean

s ‘w

eak

nes

s’ o

r ‘d

ebil

itat

ing

’ in

Zu

lu.

Ev

ery

y

ear

3 m

illi

on c

attl

e d

ie i

n A

fric

a d

ue

to N

agan

a. C

attl

e th

at c

on

trac

t th

e d

isea

se l

oo

se w

eig

ht

hav

e a

low

er c

alvin

g r

ates

, g

row

th r

etar

dat

ion

and

dif

ficu

lty

wal

kin

g. I

nfe

cted

fem

ales

can

ab

ort

an

d t

ho

se w

ho

m

anag

e to

giv

e b

irth

pro

du

ce l

ess

mil

k.

Inca

se o

f h

um

ans,

th

e in

fect

ed

per

son

may

get

a h

igh f

ever

or

hav

e a

ver

y b

ad h

ead

ach

e; v

ery

oft

en a

sw

elli

ng

ap

pea

rs a

t th

e b

ott

om

of

the

nec

k;

som

etim

es t

he

per

son

’s f

ace

may

bec

om

e ver

y p

uff

y.

Aft

er s

om

etim

e, t

he

per

son b

eco

mes

ver

y s

ick,

oft

en s

leep

s d

uri

ng t

he

day

, ea

ts v

ery

lit

tle

and

bec

om

es t

hin

or

beh

aves

li

ke

a m

ad p

erso

n. I

f n

ot

trea

ted

, th

e si

ck p

erso

n w

ill

die

.

Is S

wa

zila

nd

In

fest

ed w

ith

Tse

tse f

lies

?

Th

e an

swer

is

NO

or

YE

S.

We

do n

ot

kn

ow

un

til

a p

rop

er s

urv

ey i

s

un

der

tak

en.

Nei

ghb

ou

ring

Moza

mb

ique

and K

waz

ulu

Nat

al i

n S

outh

A

fric

a ar

e ho

wev

er,

infe

sted

and

cat

tle

mo

vin

g f

rom

thes

e co

un

trie

s co

uld

be

infe

cted

. I

n S

waz

ilan

d t

he

dis

ease

bo

th i

n c

attl

e an

d i

n h

um

ans

has

not

bee

n r

eport

ed f

or

a lo

ng t

ime.

Tse

tse

Su

rvey

Min

istr

ies

of

Ag

ricu

ltu

re a

nd

Hea

lth

of

the

Go

ver

nm

ent

of

Sw

azil

and

in

co

op

erat

ion

wit

h

Inte

rnat

ion

al C

entr

e o

f In

sect

Phy

sio

logy

and E

colo

gy

(I

CIP

E),

Nai

robi,

Ken

ya

and

wit

h t

he

sup

po

rt o

f W

HO

an

d V

este

rgaa

rd

Fra

nd

sen ,

Den

mar

kar

e p

lann

ing t

o c

onduct

a s

urv

ey i

n S

waz

ilan

d t

his

y

ear

imm

edia

tely

aft

er t

he

rain

s an

d i

n t

he

dry

sea

son

. T

he

surv

ey w

ill

be

cond

uct

ed i

n t

he

Lub

om

bo a

nd

Shis

elw

eni

reg

ions

bo

rder

ing

Mo

zam

biq

ue

and

Kw

azu

lu-N

atal

(i.

e. i

n t

he

Eas

tern

Lo

wvel

d,

Wes

tern

L

ow

vel

d a

nd

Leb

om

bo

Ran

ge)

.

Ho

w w

ill

we

catc

h t

sets

e fl

ies?

Sci

enti

sts

hav

e d

evel

op

ed b

lue

and

bla

ck c

loth

tra

ps

to c

aptu

re t

sets

e.

Blu

e an

d b

lack

colo

urs

att

ract

fli

es,

wh

ile

the

mo

squit

o n

etti

ng

lea

ds

the

flie

s to

cat

chin

g b

ott

les.

U

sing

att

ract

ive

odo

urs

e.g

. ch

emic

al

sub

stan

ces

that

mim

ic t

he

odo

ur

of

catt

le, th

e ef

fici

ency

of

the

trap

s ca

n

be

enhan

ced.

In S

waz

ilan

d t

he

‘H’

trap

dev

elo

ped

in S

outh

Afr

ica

and

supp

lied

by

Ves

terg

aard

Fra

ndse

n w

ill

be

use

d t

o c

atch

th

e fl

ies.

Try

pan

oso

mes

in b

lood

H -

Tra

p

Hu

man

bei

ng i

nfe

cted

w

ith t

rypan

oso

mes

Infe

cted

cow

Page 53: TSETSE SURVEY IN SWAZILAND

53

An

nex

8b

siS

wa

ti B

roch

ure

fo

r C

om

mu

nit

y S

ensi

tiza

tio

n

Page 54: TSETSE SURVEY IN SWAZILAND

54

Page 55: TSETSE SURVEY IN SWAZILAND

55

Annex 8c Community Sensitization

2

4

7

3

5 6

8

1

1, 2, 3 & 5: Sensitizing the community members using the brochures

4: Children assisting in trap deployment

6, 7 & 8: Demonstrating deployment of a trap and explaining how it works

Page 56: TSETSE SURVEY IN SWAZILAND

56

Annex 9 Schedule of Survey Work

Sunday 6th April 2008 Arrival of Team leader and Data Manager from ICIPE

Monday 7th Meeting with WHO and the Ministries involved to sort out logistics, timetable of activities, training schedule, formation of survey teams and purchase of expendables at Malaria Unit in Manzini

Tuesday 8th Meeting with WHO – WR, AO and AO assistant Arrival of rest ICIPE Technical Staff

Wednesday 9th Full day Training of National Staff at Siteki Hotel

Thursday 10th & Friday 11th Purchasing of Supplies and Expendables

Sat 12th April to Wed 7th May Tsetse Survey Work.

Friday 9th May Tsetse Survey Briefing with all stakeholders and future plans at Ministry of Agriculture and Cooperative Conference Room

Saturday 10th May 2008 Departure

Page 57: TSETSE SURVEY IN SWAZILAND

57

Annex 10 Typical day before and after field work

1 2

3 4

5 6

7 8

1 & 2: Map reading and identification of grids prior to departure to the field

3: Loading of vehicles

4 – 7: Daily debriefing and preparation of workplan for following day

8: Data input

Page 58: TSETSE SURVEY IN SWAZILAND

58

Annex 11 GIS MAPS:

Map 1 Grid suitability and traps deployed

Map 2 Overview map of traps with tsetse flies

Map 3 Traps with tsetse flies

Map 4 Traps with other biting flies (Stomoxys & Tabanids)

Page 59: TSETSE SURVEY IN SWAZILAND

59

Annex 12 Habitats Surveyed

1 2

3 4

5 6

7 8

1 & 2: Site where Mbuluzi River starts

3 & 4: Mbuluzi River flowing towards Mozambique in grids I 04 & J04

5 & 6: Mlawula River flowing towards Mbuluzi River in grids I 04 & I 05

7: Entrance to Mlawula Nature Reserve

8: H-Trap inside Mlawula Nature Reserve

Page 60: TSETSE SURVEY IN SWAZILAND

60

9 10

11 12

13 14

15 16

9 & 10: Habitat where G. austeni were caught in grid I 05

11: Lebombo Mountains along Mozambique border

12: Mozambique border close to where tsetse were found

13 & 14: Roads leading towards Mozambique border

15 & 16: Dry River beds crossed on foot for trap deployment

Page 61: TSETSE SURVEY IN SWAZILAND

61

17 18

19 20

21 22

23 24

17 & 18: Cultivated areas and human settlement North of Piggs Peak area (not

suitable tsetse habitats)

19 & 20: Man-made forest in Piggs Peak area (not suitable tsetse habitats)

21 & 22: Sugarcane plantations in Simunye and Lavumisa areas respectively

Page 62: TSETSE SURVEY IN SWAZILAND

62

25 26

2728

29 30

23: Cows grazing in Mlawula Nature Reserve

24: Cows in Nisela Park

25: Cattle crush in Bar Circle Ranch in grid H09 where traps were deployed

26: Cows in grid G9

27 – 30: Wild animals encountered during the survey

29 & 30: Poaching of animals along Mozambique border

Page 63: TSETSE SURVEY IN SWAZILAND

63

Annex 13 Trap deployment (steps involved)

1 2

3 4

56

7 8

1 & 2: Map reading and identification of grids to be surveyed

3, 4 & 5: Loading vehicles for survey work

6 & 7: Preparing odour dispensers

Page 64: TSETSE SURVEY IN SWAZILAND

64

9 10

11 12

13 14

15 16

8, 9 & 10: Carrying trap and associated equipment to the identified habitat for trap

deployment

11: Clearing the vegetation in a trap site

12 – 15: Trap deployment

16: Putting car-grease around support poles to protect captured flies from

predators

Page 65: TSETSE SURVEY IN SWAZILAND

65

17 18

19 20

21 22

23 24

16 – 18: Marking, numbering and geo-referencing each trap position

19: Marking trap site with a ribbon for ease of identification during sampling days

20 & 21: H-trap fully deployed

22: One of the side cones of the trap showing the fly collection bottle

23 & 24: Soldiers of the Royal Swazi Defense Force providing protection and

assisting in trap deployment

Page 66: TSETSE SURVEY IN SWAZILAND

66

Annex 14 Traps vandalized by cows

1 2

3 4

5 6

7 8

1 – 8: Traps destroyed by cows especially in grids J03 and J08 along Mozambique

border

Page 67: TSETSE SURVEY IN SWAZILAND

67

An

nex

15

Un

agg

rega

ted

Da

ta S

hee

ts

Grid

ID

J10

UT

M Z

one

36

D

ate

of tr

ap d

eplo

ymen

t 16t

h A

pril

2008

Tea

m

2

D

ata

Offi

cer

P. M

uasa

N

o.

S

AM

PLI

NG

OF

DA

Y 1

1

7 A

pr. 2

008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt*

V

eg.

Tse

tse

Biti

ng F

lies

* A

ltitu

de

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

T

he W

est s

ide

of th

e G

rid is

cul

tivat

ed

land

an

d H

omes

tead

s J1

0 T

1 26

.741

80

32.0

4795

54

2 T

hick

et

0

0 0

3 0

0

J10

T2

26.7

3939

32

.054

28

495

Thi

cket

0 0

0 2

0 1

The

trap

was

dep

loye

d in

the

valle

y to

war

ds

J10

T3

26.7

8251

32

.119

26

337

For

est

0

0 0

1 0

0

J1

0 T

4 26

.786

89

32.1

0359

38

9 F

ores

t

0 0

0 1

0 0

J10

T5

26.7

7794

32

.085

14

329

For

est

0

0 0

0 0

0

J1

0 T

6 26

.770

62

32.0

8065

35

8 F

ores

t

0 0

0 2

0 0

J10

T7

26.7

7296

32

.069

38

737

For

est

0

0 0

0 0

0

S

AM

PLI

NG

OF

DA

Y 2

1

8th

Apr

. 200

8

T

sets

e

B

iting

Flie

s

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

. S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

tom

oxys

T

aban

ids

J1

0 T

1 26

.741

80

32.0

4795

54

2 T

hick

et

0

0 0

1 0

0

J1

0 T

2 26

.739

39

32.0

5428

49

5 T

hick

et

0

0 0

0 0

0

J1

0 T

3 26

.782

51

32.1

1926

33

7 F

ores

t

0 0

0 0

0 0

J10

T4

26.7

8689

32

.103

59

389

For

est

0

0 0

0 0

0

J1

0 T

5 26

.777

94

32.0

8514

32

9 F

ores

t

0 0

0 0

0 0

J10

T6

26.7

7062

32

.080

65

358

For

est

0

0 0

1 0

0

J1

0 T

7 26

.772

96

32.0

6938

73

7 F

ores

t

0 0

0 0

0 0

S

AM

PLI

NG

OF

DA

Y 3

19t

h A

pril

2008

T

sets

e

B

iting

Flie

s

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

. S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Page 68: TSETSE SURVEY IN SWAZILAND

68

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Sto

mox

ys

Tab

anid

s

J10

T1

26.7

4180

32

.047

95

542

Thi

cket

0 0

0 3

0 0

J10

T2

26.7

3939

32

.054

28

495

Thi

cket

0 0

0 1

0 0

J10

T3

26.7

8251

32

.119

26

337

For

est

0

0 0

0 0

0

J1

0 T

4 26

.786

89

32.1

0359

38

9 F

ores

t

0 0

0 2

0 0

J10

T5

26.7

7794

32

.085

14

329

For

est

0

0 0

10

0 0

J10

T6

26.7

7062

32

.080

65

358

For

est

0

0 0

2 0

0

J1

0 T

7 26

.772

96

32.0

6938

73

7 F

ores

t

0 0

0 0

0 0

Grid

ID.

E1

UT

M Z

one

36

Dat

e 1s

t May

200

8

Tea

m

2

D

ata

Offi

cer

P. M

uasa

N

o

S

AM

PLI

NG

OF

DA

Y 1

2n

d M

ay 2

008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

T

sets

e

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

T

he g

rid is

has

sch

ools

, vill

ages

and

sm

all s

cale

farm

ing.

E

1 T

1 25

.896

88

31.5

6162

46

9 T

hick

et

0

0 0

0 0

0

E

1 T

2 25

.893

00

31.5

5887

49

6 La

nten

a B

0 0

0 0

0 0

E1

T3

25.8

7747

31

.585

15

330

Thi

cket

0 0

0 0

0 0

E1

T4

25.8

8275

31

.591

14

328

Thi

cket

0 0

0 0

0 0

Tra

p ne

ar s

wam

py a

rea

E1

T5

25.8

8403

31

.645

34

361

Thi

cket

0 0

0 3

0 2

E1

T6

25.8

8225

31

.635

98

385

Woo

dlan

d

0 0

0 2

0 0

E1

T7

52.8

8576

31

.619

29

442

Thi

cket

0 0

0 5

0 0

S

AM

PLI

NG

OF

DA

Y 2

3rd

May

200

8

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

T

sets

e

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

E

1 T

1 25

.896

88

31.5

6162

46

9 T

hick

et

0

0 0

14

0 0

E1

T2

25.8

9300

31

.558

87

496

Lant

ena

B

0

0 0

18

2 0

E1

T3

25.8

7747

31

.585

15

330

Thi

cket

0 0

0 0

0 0

E1

T4

25.8

8275

31

.591

14

328

Thi

cket

0 0

0 0

0 0

E1

T5

25.8

8403

31

.645

34

361

Thi

cket

0 0

0 0

0 0

E1

T6

25.8

8225

31

.635

98

385

Woo

dlan

d

0 0

0 0

0 0

E1

T7

52.8

8576

31

.619

29

442

Thi

cket

0 0

0 0

0 0

Page 69: TSETSE SURVEY IN SWAZILAND

69

S

AM

PLI

NG

OF

DA

Y 3

4th

May

200

8

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

E

1 T

1 25

.896

88

31.5

6162

46

9 T

hick

et

0

0 0

0 0

0

E

1 T

2 25

.893

00

31.5

5887

49

6 La

nten

a B

0 0

0 8

2 0

E1

T3

25.8

7747

31

.585

15

330

Thi

cket

0 0

0 2

0 0

E1

T4

25.8

8275

31

.591

14

328

Thi

cket

0 0

0 1

1 0

E1

T5

25.8

8403

31

.645

34

361

Thi

cket

0 0

0 2

0 0

E1

T6

25.8

8225

31

.635

98

385

Woo

dlan

d

0 0

0 7

0 0

E1

T7

52.8

8576

31

.619

29

442

Thi

cket

0 0

0 4

0 0

Grid

ID.

E2

UT

M Z

one

36

Dat

e 1s

t May

200

8

Tea

m

2

D

ata

offic

er P

. Mua

sa

No.

S

AM

PLI

NG

OF

DA

Y 1

2nd

May

200

8

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

G

rid fa

lls in

vill

ages

, sch

ools

and

be

twee

n bi

g su

garc

ane

farm

s an

d bi

sect

ed b

y riv

er N

kom

ati

E2

T1

25

.989

45

31.6

1842

38

2 T

hick

et

0

0 0

1 0

0

E

2 T

2 25

.987

62

31.6

1153

37

8 T

hick

et

0

0 0

6 0

0

E

2 T

3 25

.981

60

31.6

1010

34

6 T

hick

et

0

0 0

3 0

1

E

2 T

4 25

.977

51

31.6

0569

33

1 W

oodl

and

0

0 0

4 0

0

E

2 T

5 25

.968

30

31.5

9906

29

9 W

d/T

h

0 0

0 8

0 0

E2

T6

25.9

6089

31

.596

57

290

Thi

cket

0 0

0 4

2 0

E2

T7

25.9

6460

31

.544

43

371

Thi

cket

0 0

0 10

1

1

E

2 T

8 25

.961

10

31.5

5240

35

0 T

hick

et

V

anda

lised

SA

MP

LIN

G O

F D

AY

2

3r

d M

ay 2

008

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

E2

T1

25

.989

45

31.6

1842

38

2 T

hick

et

0

0 0

1 0

0

E

2 T

2 25

.987

62

31.6

1153

37

8 T

hick

et

0

0 0

3 0

0

E

2 T

3 25

.981

60

31.6

1010

34

6 T

hick

et

0

0 0

0 0

0

E

2 T

4 25

.977

51

31.6

0569

33

1 W

oodl

and

0

0 0

4 0

0

E

2 T

5 25

.968

30

31.5

9906

29

9 W

d/T

h

0 0

0 1

0 0

E2

T6

25.9

6089

31

.596

57

290

Thi

cket

0 0

0 2

0 0

Page 70: TSETSE SURVEY IN SWAZILAND

70

E2

T7

25.9

6460

31

.544

43

371

Thi

cket

0 0

0 2

0 0

E2

T8

25.9

6110

31

.552

40

350

Thi

cket

0 0

0

SA

MP

LIN

G O

F D

AY

3

4th

May

200

8

T

sets

e

Biti

ng F

lies

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

E2

T1

25

.989

45

31.6

1842

38

2 T

hick

et

0

0 0

0 0

0

E

2 T

2 25

.987

62

31.6

1153

37

8 T

hick

et

0

0 0

3 0

0

E

2 T

3 25

.981

60

31.6

1010

34

6 T

hick

et

0

0 0

3 1

0

E

2 T

4 25

.977

51

31.6

0569

33

1 W

oodl

and

0

0 0

0 0

2

E

2 T

5 25

.968

30

31.5

9906

29

9 W

d/T

h

0 0

0

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le tr

ap s

tole

n

E2

T6

25.9

6089

31

.596

57

290

Thi

cket

0 0

0 7

0 0

E2

T7

25.9

6460

31

.544

43

371

Thi

cket

0 0

0 0

0 0

E2

T8

25.9

6110

31

.552

40

350

Thi

cket

0 0

Grid

ID.

G10

U

TM

Zon

e 36

D

ate

15th

Apr

il 20

08

T

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Page 71: TSETSE SURVEY IN SWAZILAND

71

SA

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Page 72: TSETSE SURVEY IN SWAZILAND

72

Tse

tse

B

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Page 73: TSETSE SURVEY IN SWAZILAND

73

S

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Page 74: TSETSE SURVEY IN SWAZILAND

74

SA

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3

24th

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Page 75: TSETSE SURVEY IN SWAZILAND

75

Tse

tse

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36

Dat

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th A

pril

2008

Page 76: TSETSE SURVEY IN SWAZILAND

76

Tea

m

2

D

ata

offic

er P

. mua

sa

No.

S

AM

PLI

NG

OF

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Y 1

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Page 77: TSETSE SURVEY IN SWAZILAND

77

H10

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11

UT

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one

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Dat

e 14

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pril

2008

Tea

m

2

D

ata

offic

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S

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PLI

NG

OF

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Page 78: TSETSE SURVEY IN SWAZILAND

78

S

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Dat

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Page 79: TSETSE SURVEY IN SWAZILAND

79

Tse

tse

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Grid

ID.

I 4

UT

M Z

one

36

Dat

e 27

th A

pril

2008

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m

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28

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29

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Page 80: TSETSE SURVEY IN SWAZILAND

80

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OF

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Y 3

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Page 81: TSETSE SURVEY IN SWAZILAND

81

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Page 82: TSETSE SURVEY IN SWAZILAND

82

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Page 83: TSETSE SURVEY IN SWAZILAND

83

Tra

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ys

Tab

anid

s

B1

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25.9

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31

.306

63

813

For

est

0

0 0

0 0

1 H

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.858

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0

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.867

18

31.3

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.874

90

31.3

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NG

OF

TR

AP

S D

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3

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(m)

Typ

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T

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F

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s R

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anid

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est

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Page 84: TSETSE SURVEY IN SWAZILAND

84

Grid

ID.

D1

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Tab

anid

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D1

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Page 85: TSETSE SURVEY IN SWAZILAND

85

F3

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Tab

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97

31.7

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69

31.7

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26.0

6483

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Tab

anid

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F3

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1094

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31.7

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Page 86: TSETSE SURVEY IN SWAZILAND

86

F3

T11

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TR

AP

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29

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(x)

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C

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Page 87: TSETSE SURVEY IN SWAZILAND

87

SA

MP

LIN

G O

F T

RA

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DA

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30th

Apr

il 20

08

T

sets

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Grid

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e

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anid

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F15

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88

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

19th

Apr

il 20

08

T

sets

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Page 89: TSETSE SURVEY IN SWAZILAND

89

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

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cies

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Page 90: TSETSE SURVEY IN SWAZILAND

90

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

26th

Apr

il 20

08

T

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Page 91: TSETSE SURVEY IN SWAZILAND

91

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T5

26.3

4849

31

.801

36

317

Thi

cket

0 0

0 2

0 0

G6

T6

26.3

1843

31

.783

49

294

Thi

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0 0

0 4

1 5

Hou

sefli

es

G

6 T

7 26

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94

31.7

8209

28

8 W

oodl

and

0

0 0

3 0

4 H

ouse

flies

G6

T8

26.3

2294

31

.781

27

293

Thi

cket

0 0

0 0

0 3

Hou

sefli

es a

nd b

utte

rfly

G

6 T

9 26

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87

31.7

7872

30

8 T

hick

et

0

0 0

0 0

2 H

ouse

flies

G6

T10

26

.326

49

31.7

7757

31

6 T

hick

et

0

0 0

6 0

6 H

ouse

flies

G6

T11

26

.328

52

31.7

7580

32

1 T

hick

et

0

0 0

12

0 5

Hou

sefli

es

G

6 T

12

26.3

3462

31

.768

25

328

Thi

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0 0

0 9

0 9

Hou

sefli

es

G

rid ID

. G

8

U

TM

Zon

e 36

D

ate

22nd

Apr

il 20

08

T

eam

1

Dat

a of

ficer

J. A

ndok

e

N

o.

SA

MP

LIN

G O

F D

AY

1

23

rd A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

G8

T1

26.5

4378

31

.819

10

241

Woo

dlan

d

0 0

0 10

0

8 W

oodl

and

neig

hbor

ing

culti

vate

d fie

lds

(Hou

sefli

es)

G8

T2

26.5

4365

31

.821

84

250

Woo

dlan

d

0 0

0 0

0 0

G8

T3

26.5

6390

31

.823

69

236

Woo

dlan

d

0 0

0 1

0 0

Alo

ng th

e riv

er

G8

T4

26.5

6127

31

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81

240

Thi

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0 0

0 0

0 1

Priv

ate

Ran

ch

G

8 T

5 26

.561

04

31.8

1105

24

1 T

hick

et

0

0 0

1 0

1 In

side

the

Ran

ch (

Hou

sefly

)

Page 92: TSETSE SURVEY IN SWAZILAND

92

G8

T6

26.5

6422

31

.805

61

260

Thi

cket

0 0

0 5

0 3

Insi

de th

e R

anch

(H

ouse

flies

, was

p an

d be

e)

G8

T7

26.5

6525

31

.801

81

263

Thi

cket

0 0

0 7

0 3

Insi

de th

e R

anch

- C

ows

tam

pere

d w

ith

trap

G

8 T

8 26

.561

84

31.7

8721

27

4 T

hick

et

0

0 0

3 0

4 In

side

the

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ch (

Hou

sefli

es)

G8

T9

26.5

6204

31

.790

74

263

Thi

cket

0 0

0 1

0 1

Insi

de th

e R

anch

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ouse

fly)

G8

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26

.563

30

31.7

9534

24

9 T

hick

et

0

0 0

0 0

2 In

side

the

Ran

ch (

Hou

sefli

es)

SA

MP

LIN

G O

F T

RA

PS

DA

Y 2

24th

Apr

il 20

08

T

sets

e

Biti

ng F

lies

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

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o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

G8

T1

26.5

4378

31

.819

10

241

Woo

dlan

d

0 0

0 7

0 7

Hou

sefli

es

G

8 T

2 26

.543

65

31.8

2184

25

0 W

oodl

and

0

0 0

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0

G

8 T

3 26

.563

90

31.8

2369

23

6 W

oodl

and

0

0 0

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0

G

8 T

4 26

.561

27

31.8

1481

24

0 T

hick

et

0

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0 0

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flies

G8

T5

26.5

6104

31

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05

241

Thi

cket

0 0

0 1

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G8

T6

26.5

6422

31

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61

260

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0 0

0 2

0 2

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sefli

es

G

8 T

7 26

.565

25

31.8

0181

26

3 T

hick

et

0

0 0

5 0

2 H

ouse

flies

G8

T8

26.5

6184

31

.787

21

274

Thi

cket

0 0

0 2

0 3

Hou

sefli

es

G

8 T

9 26

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04

31.7

9074

26

3 T

hick

et

0

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asp

G

8 T

10

26.5

6330

31

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34

249

Thi

cket

0 0

0 0

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sefly

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

25th

Apr

il 20

08

T

sets

e

Biti

ng F

lies

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

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o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

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mox

ys

Tab

anid

s

G8

T1

26.5

4378

31

.819

10

241

Woo

dlan

d

0 0

0 3

0 3

Hou

sefli

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G

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65

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2184

25

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and

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G

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90

31.8

2369

23

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0

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27

31.8

1481

24

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et

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0

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8 T

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04

31.8

1105

24

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0

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0 0

0

G

8 T

6 26

.564

22

31.8

0561

26

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et

0

0 0

1 0

1 H

ouse

flies

G8

T7

26.5

6525

31

.801

81

263

Thi

cket

0 0

0 0

0 2

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sefli

es

G

8 T

8 26

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84

31.7

8721

27

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et

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0

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04

31.7

9074

26

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0

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0

G

8 T

10

26.5

6330

31

.795

34

249

Thi

cket

0 0

0 0

0 1

Fru

itfly

Grid

ID.

G9

UT

M Z

one

36

Dat

e 22

nd A

pril

2008

Tea

m

1

D

ata

offic

er J

. And

oke

No.

Page 93: TSETSE SURVEY IN SWAZILAND

93

S

AM

PLI

NG

OF

DA

Y 1

23rd

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

Sou

th s

ide

of th

e gr

id -

Hom

este

ads

and

culti

vate

d la

nd

G9

T1

26

.695

21

31.7

6374

19

0 T

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0

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rap

set a

fter

hom

este

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ith c

rush

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G

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T3

26.6

9444

31

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52

175

Thi

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26.6

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18

234

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0 2

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Bug

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26.6

7904

31

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232

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0 0

0 2

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26.6

7709

31

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92

217

Thi

cket

0 0

0 2

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26.6

7189

31

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28

217

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0 3

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Ant

and

spi

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G

9 T

9 26

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29

31.8

0186

21

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0

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rap

near

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bur

row

s (H

ouse

fly)

SA

MP

LIN

G O

F T

RA

PS

DA

Y 2

24th

Apr

il 20

08

T

sets

e

Biti

ng F

lies

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

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N

o.

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rdin

ates

C

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es

(m)

Typ

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peci

es

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otal

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ther

s R

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Tab

anid

s

G9

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26

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31.7

6374

19

0 T

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G

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44

31.7

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18

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26.6

9444

31

.773

52

175

Thi

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0 0

0 2

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26.6

8307

31

.773

78

205

Thi

cket

0 0

0 0

0 0

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T5

26.6

8129

31

.786

18

234

Thi

cket

0 0

0 1

0 0

G9

T6

26.6

7904

31

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95

232

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cket

0 0

0 2

0 0

G9

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26.6

7709

31

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92

217

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0 0

0 1

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G9

T8

26.6

7189

31

.797

28

217

Thi

cket

0 0

0 1

0 0

G9

T9

26.6

7329

31

.801

86

211

Thi

cket

0 0

0 5

0 1

Hou

sefly

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

25th

Apr

il 20

08

T

sets

e

Biti

ng F

lies

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rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

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o.

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rdin

ates

C

oord

inat

es

(m)

Typ

e S

peci

es

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otal

F

amily

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ther

s R

emar

ks

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mox

ys

Tab

anid

s

G9

T1

26

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21

31.7

6374

19

0 T

hick

et

0

0 0

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0

G

9 T

2 26

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44

31.7

7123

18

1 T

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0

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19

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26.6

9444

31

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52

175

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0 5

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flies

and

1 m

oth

G9

T4

26.6

8307

31

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78

205

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0 0

0 1

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26.6

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31

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18

234

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0 0

0 2

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sefly

G9

T6

26.6

7904

31

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95

232

Thi

cket

0 0

0 2

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sefly

G9

T7

26.6

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31

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92

217

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26.6

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31

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28

217

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flies

and

was

p G

9 T

9 26

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29

31.8

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21

1 T

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et

0

0 0

2 0

0

Page 94: TSETSE SURVEY IN SWAZILAND

94

Grid

ID.

G15

U

TM

Zon

e 36

D

ate

13th

and

15

Apr

il 20

08

T

eam

1

Dat

a of

ficer

J. A

ndok

e

N

o.

SA

MP

LIN

G O

F D

AY

1

16

th A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

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Flie

s

No.

C

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inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

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al

Fam

ily

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S

tom

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G

15 T

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42

31.8

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31

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58

337

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Not

e N

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appl

ied

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ac

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e

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MP

LIN

G O

F T

RA

PS

DA

Y 2

17th

Apr

il 20

08

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p

Latit

ude(

y)

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itude

(x)

Alt

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.

T

sets

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ates

(m

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84

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31

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58

337

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40

378

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and

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0

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`

SA

MP

LIN

G O

F T

RA

PS

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Y 3

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08

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de(x

) A

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sets

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ng F

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rdin

ates

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es

(m)

Typ

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S

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es

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amily

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ther

s R

emar

ks

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ys

Tab

anid

s

G15

T1

27.2

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31

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49

335

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ssla

nd

0

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84

31.7

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43

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27.2

9866

31

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58

337

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0

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1 4

Page 95: TSETSE SURVEY IN SWAZILAND

95

G15

T4

27.2

9035

31

.788

40

378

Gra

ssla

nd

0

0 0

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ther

s -

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sefli

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G15

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27.2

9952

31

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398

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0

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0

G

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18

31.7

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30

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and

0

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fly

G

15 T

7 27

.290

03

31.7

6703

35

2 T

hick

et

0

0 0

3 0

2 H

ouse

flies

Grid

ID.

H4

UT

M Z

one

36

Dat

e 28

th A

pril

2008

Tea

m

1

HLA

NE

RO

YA

L N

AT

ION

AL

PA

RK

ON

TH

E N

OR

TH

ER

N P

AR

T

No.

Dat

a of

ficer

J. A

ndok

e

SA

MP

LIN

G O

F D

AY

1

29

th A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

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.

Tse

tse

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Flie

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C

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inat

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ates

(m

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Spe

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Tot

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Rem

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S

tom

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T

aban

ids

H

4 T

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42

31.9

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26

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der

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.198

32

31.9

0012

25

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land

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flies

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26.1

9764

31

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45

256

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ssla

nd

0

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flies

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T4

26.1

9965

31

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07

254

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0

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flies

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26.1

9638

31

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40

233

Gra

ssla

nd

0

0 0

1 1

0

H

4 T

6 26

.194

26

31.8

8499

24

0 G

rass

land

0 0

0 1

1 1

Hou

sefly

H4

T7

26.1

9657

31

.881

92

247

Gra

ssla

nd

0

0 0

0 2

1 H

ouse

fly

H

4 T

8 26

.197

79

31.8

7807

26

6 G

rass

land

0 0

0 1

1 1

Hou

sefly

H4

T9

26.1

9338

31

.878

17

256

Gra

ssla

nd

0

0 0

1 3

3 2

mot

hs 1

spi

der

H4

10

26.1

8799

31

.877

74

238

Gra

ssla

nd

0

0 0

6 3

0

H

4 T

11

26.1

8259

31

.871

62

243

Gra

ssla

nd

0

0 0

0 0

2 S

pide

r an

d m

oth

H4

T12

26

.181

07

31.8

6362

24

8 T

hick

et

0

0 0

3 1

0

H

4 T

13

26.1

7382

31

.859

11

221

Thi

cket

0 0

0 0

1 1

Hou

sefly

SA

MP

LIN

G O

F T

RA

PS

DA

Y 2

30th

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

H4

T1

26.2

0142

31

.902

03

263

Gra

ssla

nd

0

0 0

0 1

0

H

4 T

2 26

.198

32

31.9

0012

25

8 G

rass

land

0 0

0 5

1 30

H

ouse

flies

H4

T3

26.1

9764

31

.897

45

256

Gra

ssla

nd

0

0 0

0 2

1 H

ouse

fly

H

4 T

4 26

.199

65

31.8

9407

25

4 G

rass

land

0 0

0 2

0 10

H

ouse

flies

H4

T5

26.1

9638

31

.888

40

233

Gra

ssla

nd

0

0 0

0 1

0

H

4 T

6 26

.194

26

31.8

8499

24

0 G

rass

land

0 0

0 1

2 1

Hou

sefly

H4

T7

26.1

9657

31

.881

92

247

Gra

ssla

nd

0

0 0

0 2

1 H

ouse

fly

H

4 T

8 26

.197

79

31.8

7807

26

6 G

rass

land

0 0

0 0

1 1

Hou

sefly

H4

T9

26.1

9338

31

.878

17

256

Gra

ssla

nd

0

0 0

0 1

1 H

ouse

fly

H

4 10

26

.187

99

31.8

7774

23

8 G

rass

land

0 0

0 0

1 0

H4

T11

26

.182

59

31.8

7162

24

3 G

rass

land

0 0

0 0

1 0

H4

T12

26

.181

07

31.8

6362

24

8 T

hick

et

0

0 0

0 0

0

Page 96: TSETSE SURVEY IN SWAZILAND

96

H4

T13

26

.173

82

31.8

5911

22

1 T

hick

et

0

0 0

1 0

0

S

AM

PLI

NG

OF

TR

AP

S D

AY

3

1s

t May

200

8

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

H

4 T

1 26

.201

42

31.9

0203

26

3 G

rass

land

0 0

0 0

0 2

Hou

sefli

es

H

4 T

2 26

.198

32

31.9

0012

25

8 G

rass

land

0 0

0 4

1 29

H

ouse

flies

H4

T3

26.1

9764

31

.897

45

256

Gra

ssla

nd

0

0 0

1 1

25

Hou

sefli

es

H

4 T

4 26

.199

65

31.8

9407

25

4 G

rass

land

0 0

0 5

5 51

H

ouse

flies

H4

T5

26.1

9638

31

.888

40

233

Gra

ssla

nd

0

0 0

0 1

1 S

pide

r

H4

T6

26.1

9426

31

.884

99

240

Gra

ssla

nd

0

0 0

1 0

1 H

ouse

fly

H

4 T

7 26

.196

57

31.8

8192

24

7 G

rass

land

0 0

0 1

2 0

H4

T8

26.1

9779

31

.878

07

266

Gra

ssla

nd

0

0 0

2 0

1 H

ouse

fly

H

4 T

9 26

.193

38

31.8

7817

25

6 G

rass

land

0 0

0 1

0 1

Hou

sefly

H4

10

26.1

8799

31

.877

74

238

Gra

ssla

nd

0

0 0

0 0

2 H

ouse

flies

H4

T11

26

.182

59

31.8

7162

24

3 G

rass

land

0 0

0 0

1 3

1 B

utte

rfly

H4

T12

26

.181

07

31.8

6362

24

8 T

hick

et

0

0 0

0 1

0

H

4 T

13

26.1

7382

31

.859

11

221

Thi

cket

0 0

0 1

0 1

Hou

sefly

Grid

ID.

H5

UT

M Z

one

36

Dat

e 23

rd A

pril

2008

Tea

m

1

D

ata

offic

er J

. And

oke

No.

GR

ID IN

SID

E H

LAN

E R

OY

AL

NA

TIO

NA

L P

AR

K

S

AM

PLI

NG

OF

DA

Y 1

24th

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

Tra

ps 1

-8 C

ow u

rine

+ ac

eton

e

Tra

ps 1

-10

depl

oyed

on

the

Wes

t sid

e of

th

e gr

id

H5

T1

26.2

4951

31

.875

09

274

Thi

cket

0 0

0 0

0 2

Gra

ssho

pper

and

mot

h H

5 T

2 26

.245

21

31.8

7503

26

7 T

hick

et

0

0 0

1 1

3 1

hous

efly

, 1 m

oth

and

1 w

asp

H5

T3

26.2

3599

31

.874

55

252

Thi

cket

0 0

0 3

1 4

2 ho

usef

lies

1 be

e an

d 1

ant

H5

T4

26.2

2680

31

.874

26

277

Thi

cket

0 0

0 0

0 1

Spi

der

H

5 T

5 26

.217

14

31.8

7423

27

5 T

hick

et

0

0 0

3 4

0

H

5 T

6 26

.261

50

31.8

1390

28

3 T

hick

et

0

0 0

0 0

2 1

But

terf

ly 1

spi

der

H5

T7

26.2

6634

31

.869

83

270

Thi

cket

0 0

0 2

0 1

Mot

h

H5

T8

26.2

7643

31

.866

93

287

Thi

cket

0 0

0 3

5 0

H5

T9

26.2

8386

31

.859

14

280

Thi

cket

0 0

0 2

0 0

H5

T10

26

.280

90

31.8

5249

28

6 T

hick

et

0

0 0

7 0

0 T

rap

near

wat

er h

ole

H5

T11

26

.281

89

31.8

8320

29

8 W

oodl

and

0

0 0

8 0

0

H5

T12

26

.283

17

31.8

8704

28

3 T

hick

et

0

0 0

7 0

2 T

rap

insi

de s

mal

l drie

d riv

er b

ed (

1 be

etle

&

mos

quito

)

Page 97: TSETSE SURVEY IN SWAZILAND

97

H5

T13

26

.283

06

31.8

9531

26

7 T

hick

et

0

0 0

3 0

1 G

rass

hopp

er a

nd m

oth

H5

T14

26

.315

00

31.8

8289

31

5 W

oodl

and

0

0 0

0 0

4 2

ants

and

2 b

utte

rflie

s H

5 T

15

26.3

1254

31

.888

76

303

Thi

cket

0 0

0 15

0

2 H

ouse

flies

H5

T16

26

.262

18

31.8

9823

26

7 T

hick

et

0

0 0

3 2

1 B

utte

rfly

H5

T17

26

.249

51

31.9

0253

28

6 T

hick

et

0

0 0

4 1

0

S

AM

PLI

NG

OF

TR

AP

S D

AY

2

25

th A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

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ers

Rem

arks

S

tom

oxys

T

aban

ids

H

5 T

1 26

.249

51

31.8

7509

27

4 T

hick

et

0

0 0

1 0

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spid

er 1

hou

sefly

H

5 T

2 26

.245

21

31.8

7503

26

7 T

hick

et

0

0 0

0 0

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snai

l 3 a

nts

1 bu

tterf

ly

H5

T3

26.2

3599

31

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55

252

Thi

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0 0

0 2

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der

and

gras

shop

per

H5

T4

26.2

2680

31

.874

26

277

Thi

cket

0 0

0 0

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1 sp

ider

and

1 a

nt

H5

T5

26.2

1714

31

.874

23

275

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cket

0 0

0 2

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tle

H

5 T

6 26

.261

50

31.8

1390

28

3 T

hick

et

0

0 0

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0

H

5 T

7 26

.266

34

31.8

6983

27

0 T

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et

0

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fly a

nd a

nt

H5

T8

26.2

7643

31

.866

93

287

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cket

0 0

0 0

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g be

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s

H5

T9

26.2

8386

31

.859

14

280

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cket

0 0

0 0

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Dun

g be

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H5

T10

26

.280

90

31.8

5249

28

6 T

hick

et

0

0 0

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0

H

5 T

11

26.2

8189

31

.883

20

298

Woo

dlan

d

0 0

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usef

lies

and

1 gr

assh

oppe

r H

5 T

12

26.2

8317

31

.887

04

283

Thi

cket

0 0

0 3

1 0

H5

T13

26

.283

06

31.8

9531

26

7 T

hick

et

0

0 0

9 1

0

H

5 T

14

26.3

1500

31

.882

89

315

Woo

dlan

d

0 0

0 0

0 3

2 bu

tterf

lies

and

1 ho

usef

ly

H5

T15

26

.312

54

31.8

8876

30

3 T

hick

et

0

0 0

7 0

1 B

utte

rfly

H5

T16

26

.262

18

31.8

9823

26

7 T

hick

et

0

0 0

3 0

1 W

asp

H

5 T

17

26.2

4951

31

.902

53

286

Thi

cket

0 0

0 1

0 2

Spi

der

and

ant

S

AM

PLI

NG

OF

TR

AP

S D

AY

3

26

th A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

H

5 T

1 26

.249

51

31.8

7509

27

4 T

hick

et

0

0 0

0 0

3 S

pide

r m

osqu

ito a

nd b

utte

rfly

H

5 T

2 26

.245

21

31.8

7503

26

7 T

hick

et

0

0 0

0 0

0

H

5 T

3 26

.235

99

31.8

7455

25

2 T

hick

et

0

0 0

2 1

1 B

utte

rfly

H5

T4

26.2

2680

31

.874

26

277

Thi

cket

0 0

0 0

0 1

Spi

der

H5

T5

26.2

1714

31

.874

23

275

Thi

cket

0 0

0 0

2 1

Hou

sefly

H5

T6

26.2

6150

31

.813

90

283

Thi

cket

0 0

0 0

0 0

H5

T7

26.2

6634

31

.869

83

270

Thi

cket

0 0

0 2

0 2

Bet

tles

H

5 T

8 26

.276

43

31.8

6693

28

7 T

hick

et

0

0 0

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ung

beet

les

H

5 T

9 26

.283

86

31.8

5914

28

0 T

hick

et

0

0 0

2 1

2 B

utte

rfly

and

mot

h H

5 T

10

26.2

8090

31

.852

49

286

Thi

cket

0 0

0 1

0 0

H5

T11

26

.281

89

31.8

8320

29

8 W

oodl

and

0

0 0

5 1

4 H

ouse

flies

H5

T12

26

.283

17

31.8

8704

28

3 T

hick

et

0

0 0

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1 M

oth

Page 98: TSETSE SURVEY IN SWAZILAND

98

H5

T13

26

.283

06

31.8

9531

26

7 T

hick

et

0

0 0

3 1

1 G

rass

hopp

er

H

5 T

14

26.3

1500

31

.882

89

315

Woo

dlan

d

0 0

0 2

2 2

Ant

s

H5

T15

26

.312

54

31.8

8876

30

3 T

hick

et

0

0 0

16

0 0

H5

T16

26

.262

18

31.8

9823

26

7 T

hick

et

0

0 0

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1 W

asp

H

5 T

17

26.2

4951

31

.902

53

286

Thi

cket

0 0

0 2

3 1

Hou

sefly

Grid

ID.

I 3

UT

M Z

one

36

Dat

e 26

th A

pril

2008

Tea

m

1

D

ata

offic

er J

. And

oke

No.

S

AM

PLI

NG

OF

DA

Y 1

27th

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

oord

inat

es

(m)

Typ

e

S

peci

es

T

otal

F

amily

O

ther

s R

emar

ks

Sto

mox

ys

Tab

anid

s

Hill

y T

erra

in a

nd V

alle

ys

I 3 T

01

26.0

8756

31

.995

58

219

Thi

cket

0 0

0 50

1

7 H

ouse

flies

I 3 T

02

26.0

8492

31

.994

24

218

Thi

cket

0 0

0 62

0

26

Hou

sefli

es

I 3

T03

26

.084

40

31.9

9221

23

7 T

hick

et

0

0 0

0 0

7 H

ouse

flies

I 3 T

04

26.0

8196

31

.990

18

246

Gra

ssla

nd

0

0 0

7 1

14

Hou

sefli

es

I 3

T05

26

.080

46

31.9

8812

24

9 G

rass

land

0 0

0 5

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Tam

pere

d w

ith

I 3 T

06

26.0

3840

31

.983

34

403

Thi

cket

0 0

0 4

0 6

Hou

sefli

es

I 3

T07

26

.034

19

31.9

7894

39

4 T

hick

et

0

0 0

11

0 16

H

ouse

flies

I 3 T

08

26.0

3059

31

.972

52

391

Thi

cket

0 0

0 19

0

22

Hou

sefli

es

S

AM

PLI

NG

OF

TR

AP

S D

AY

2

28

th A

pril

2008

Tra

p

Latit

ude(

y)

Long

itude

(x)

Alt

Veg

.

Tse

tse

B

iting

Flie

s

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

Oth

ers

Rem

arks

S

tom

oxys

T

aban

ids

I 3

T 0

1 26

.087

56

31.9

9558

21

9 T

hick

et

0

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5 H

ouse

flies

I 3 T

02

26.0

8492

31

.994

24

218

Thi

cket

0 0

0 8

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ouse

flies

I 3 T

03

26.0

8440

31

.992

21

237

Thi

cket

0 0

0 0

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Hou

sefli

es

I 3

T04

26

.081

96

31.9

9018

24

6 G

rass

land

0 0

0 1

0 1

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sefli

es

I 3

T05

26

.080

46

31.9

8812

24

9 G

rass

land

0 0

0 42

0

5 H

ouse

flies

I 3 T

06

26.0

3840

31

.983

34

403

Thi

cket

0 0

0 1

0 3

1 w

asp

and

hous

eflie

s I 3

T07

26

.034

19

31.9

7894

39

4 T

hick

et

0

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20

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sefli

es

I 3

T08

26

.030

59

31.9

7252

39

1 T

hick

et

0

0 0

19

0 26

H

ouse

flies

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

29th

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

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Page 99: TSETSE SURVEY IN SWAZILAND

99

I 3 T

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Page 100: TSETSE SURVEY IN SWAZILAND

100

SA

MP

LIN

G O

F T

RA

PS

DA

Y 3

21st

Apr

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08

T

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Grid

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D

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Page 101: TSETSE SURVEY IN SWAZILAND

101

H15

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Page 102: TSETSE SURVEY IN SWAZILAND

102

I15

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rid ID

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TM

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ate

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D

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S

AM

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NG

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Page 103: TSETSE SURVEY IN SWAZILAND

103

J8 T

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ng F

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Page 104: TSETSE SURVEY IN SWAZILAND

104

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

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ily

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Page 105: TSETSE SURVEY IN SWAZILAND

105

I5 T

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Page 106: TSETSE SURVEY IN SWAZILAND

106

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Page 107: TSETSE SURVEY IN SWAZILAND

107

I5 T

29

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Page 108: TSETSE SURVEY IN SWAZILAND

108

Grid

ID.

I6

UT

M Z

one

36

Dat

e 22

nd A

pril

2008

Tea

m

2

D

ata

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cer

P. M

uasa

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o.

SA

MP

LIN

G O

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1

23

rd A

pril

2008

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p

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3

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Page 109: TSETSE SURVEY IN SWAZILAND

109

No.

C

oord

inat

es

Coo

rdin

ates

(m

) T

ype

Spe

cies

Tot

al

Fam

ily

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Grid

ID.

J3

UT

M Z

one

36

Dat

e 27

th A

pril

2008

Tea

m

2

G

RID

IN S

HE

WU

LA M

ILIT

AR

Y C

AM

P

1

N

o.

D

ata

Offi

cer

P. M

uasa

SA

MP

LIN

G O

F D

AY

1

28

th A

pril

2008

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p

Latit

ude(

y)

Long

itude

(x)

Alt

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.

Tse

tse

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s

No.

C

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inat

es

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rdin

ates

(m

) T

ype

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cies

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al

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ily

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arks

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tom

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W

est o

f the

grid

sm

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26

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87

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Page 110: TSETSE SURVEY IN SWAZILAND

110

SA

MP

LIN

G O

F D

AY

3

30th

Apr

il 20

08

T

rap

La

titud

e(y)

Lo

ngitu

de(x

) A

lt V

eg.

T

sets

e

Biti

ng F

lies

N

o.

Coo

rdin

ates

C

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es

(m)

Typ

e

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T

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Page 111: TSETSE SURVEY IN SWAZILAND

111

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Page 112: TSETSE SURVEY IN SWAZILAND

112

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Page 113: TSETSE SURVEY IN SWAZILAND

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114

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115

Annex 16 Summary Data Sheet

Overall insects caught in traps deployed in various grids sampled

No of insects caughtGrid No No of Traps

deployed Tsetse Flies Stomoxys Tabanids Others

B1 4 0 11 0 33 D1 2 0 8 0 9 F3 14 0 18 15 57 F8 11 0 27 0 36 F15 5 0 175 4 56 G3 7 0 13 9 31 G5 9 0 82 9 130 G6 12 0 84 4 128 G8 10 0 54 0 47 G9 9 0 138 3 17 G13 0 0 0 0 0 G14 0 0 0 0 0 G15 7 0 200 4 36 H4 13 0 47 45 205 H5 17 0 139 33 74 H13 0 0 0 0 0 H15 11 0 78 28 412 I2 0 0 0 0 0 I3 8 0 275 4 205 I13 10 0 118 18 143 I14 0 0 0 0 0 I15 7 0 103 51 248 J8 8 0 108 2 30 J9 4 0 65 2 17 E1 7 0 66 5 2 E2 8 0 62 4 4

G10 2 0 72 0 2 G11 7 0 102 3 13 H6 5 0 30 0 0 H7 9 0 24 0 2 H9 16 0 1027 13 9 H10 9 0 205 1 0 H11 4 0 31 0 1 H12 10 0 186 7 30 I4 9 0 273 13 6 I5 73 7 582 77 312 I6 11 0 30 1 3 I11 0 0 0 0 0 I12 0 0 0 0 0 J3 6 0 163 3 0 J4 7 0 291 0 0 J5 3 0 52 0 0 J6 5 0 24 0 1 J7 6 0 38 0 1

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J10 7 0 39 0 1 TOTAL 372 7 5032 330 2301

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117

Annex 17 GIS files and Maps produced in the CD

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Annex 18a) List of Participants for the Training of National Staff Wednesday 9

th April 2008, Siteki Hotel Swaziland

NAME AFFILIATION CONTACT ADDRESS Dr. Roland X. Dlamini Deputy Director

Veterinary Services MoAC

P.O.Box 162, Mbabane [email protected]

Zimon Mohale Animal Health Inspector MoAC

Tel: 268 6324053

Samson Manana Animal Health Inspector MoAC

Cell: 268 6081971 P.O.Box 43, Siteki

Vusie m’ Khumalo Animal Health Inspector MoAC

Cell: 268 6130720; Fax: 268 5056443 P.O.Box 4192, Manzini

Nandla Mkhonta Cartographic Draughtsman MoAC

Tel: 268 6410435 MANDLA.MKONTA@GMAIL .COM

Dr. Mduduzi P. Dlamini

Epidemiologist (Veterinary) Department of Vet. Services MoAC

Tel: 268 5056443 / 5052113 / 6041444 P.O.Box 3287, Manzini [email protected]

Augustine Motsa Veterinary Assistant MoAC

Cell: 268 6242040 P.O.Box 43, Siteki

Dr. Nhlanhla Shongwe Ministry of Agr. & Corp (MoAC) Regional Veterinary Officer Department of Vet. Services

Tel: 268 3434268 / 6068702; P.O.Box 43, Siteki [email protected] ; [email protected]

Petros N. Sihlabela Animal Husbandry Officer Animal Production Vet. Department

P.O.Box 42, Siteki

Dr. N.E.Chikuni Veterinary Officer Siteki

P.O.Box 43, Siteki [email protected]

Paulos M. Khoza Ministry of Health (MoH) Health Assistant Unit Malaria Health Unit

Cell: 268 6079372 P.O.Box 53, Manzini

Joel M. Matse Health Assistant Malaria Health Unit MoH

Cell: 268 6082155 P.O.Box 53, Manzini

Musa Mndzebele Health Assistant Malaria Health Unit MoH

Cell: 268 6352601 P.O.Box 53, Manzini

Albert Mkhabela Field Assistant Malaria Health Unit MoH

Cell: 268 6352601 P.O.Box 53, Manzini

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119

Abel B. Matse Field Assistant Malaria Health Unit MoH

Cell: 268 6384109 P.O.Box 53, Manzini

Fanyana P. Gamedze Field Assistant Malaria Health Unit MoH

Cell: 268 6478797 P.O.Box 53, Manzini

Mtensitoli Mazibulo Data Clerk Malaria Health Unit

Cell: 268 6088917 P.O.Box 53, Manzini

Zulisile Zulu Malaria Health Researcher Malaria Health Unit

Cell: 268 6284153 P.O.Box 53, Manzini [email protected]

Thamsanqa Dlamini ICT Officer WHO

Cell: 268 6240832 / 4042928 [email protected]

David Camp Transport Assistant World Health Organization Cell: +268 6237447 P.O.Box 903, Mbabane

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Annex 18b) Capacity Building

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1: Dr. Roland X. Dlamini, Deputy Director of Veterinary Services (MoAC)

opening the training workshop

2 – 5: Participants from MoAC, MoH and WHO Regional Office attending the

training workshop

5: Registration desk

6 – 8: Demonstration on trap deployment

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Annex 19 Programme for Tsetse Debriefing Meeting

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Annex 20 List of Stakeholders Present in the Tsetse Debriefing Meeting Friday 9

th May 2008, Ministry of Agriculture Conference Room

NAME AFFILIATION CONTACT ADDRESS Dr. Robert Dlamini Director

Veterinary Services MoAC

Tel: +268 4042731 P.O.Box 162, Mbabane [email protected]

Dr. Roland X. Dlamini Deputy Director Veterinary Services MoAC

Tel: +268 4042731 Cell: +268 6087734 P.O.Box 162, Mbabane [email protected]

Dr. S. U. Magagula Deputy Director Ministry of Health MoH

Tel: +268 4045554 Cell: +268 6062708 [email protected]

Mr. Sipho A. Nzuwalu MoAC Permanent Secretary

Tel: +268 4042731 Cell: +268 6062605 P.O.Box 162, Mbabane

Mr. Dumisani Mngomezulu MoAC Permanent Secretary

Tel: +268 4042731 Cell: +268 6216477 P.O.Box 162, Mbabane

Samson Manana Animal Health Inspector MoAC

Cell: +268 6081971 P.O.Box 43, Siteki

Dr. Benjamin Gama WHO Tel: +268 4042928 Cell: +268 6224039

Gumedze Sandle SNTC NEEP Cell: +268 6053476 [email protected]

Augustine Motsa Veterinary Assistant MoAC

Cell: +268 6242040 P.O.Box 43, Siteki

Vusie m’ Khumalo Animal Health Inspector MoAC

Cell: +268 6130720; Fax: +268 5056443 P.O.Box 4192, Manzini

George Mbatha Big Game Parks Tel: +268 5283943 [email protected]

Dr. S. Mdluli Epidemiologist (Veterinary) Department of Vet. Services MoAC

Tel: +268 5056443 P.O.Box 3287, Manzini [email protected]

Dr. Mduduzi P. Dlamini Epidemiologist (Veterinary) Department of Vet. Services MoAC

Tel: +268 5056443 / 5052113 / 6041444 P.O.Box 3287, Manzini [email protected]

Paulos M. Khoza Ministry of Health (MoH) Health Assistant Unit Malaria Health

Cell: +268 6079372 P.O.Box 53, Manzini

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Joel M. Matse Health Assistant Malaria Health Unit MoH

Cell: +268 6082155 P.O.Box 53, Manzini

Musa Mndzebele Health Assistant Malaria Health Unit MoH

Cell: +268 6352601 P.O.Box 53, Manzini

Dr. Rajinder K. Saini Principal Investigator ICIPE Nairobi

Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya [email protected]

Caroline Muthoni Muya Data Manager ICIPE Nairobi

Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

John Andoke Akiri Tsetse Entomologist ICIPE Nairobi

Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

Peter Nthale Muasa Tsetse Entomologist ICIPE Nairobi

Tel: +254 863200 P.O.Box 30772, 0100 Nairobi, Kenya

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Annex 21 End of Survey Party Hosted by Principal Investigator

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