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TTh  11:00-12:15 in Clark S361

Profs: Serafim Batzoglou, Gill Bejerano

TAs: George Asimenos, Cory McLean

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Lecture 13

Insulators in the Human Genome

Discuss Project

Co-option

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Insulators: A Reminder

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CTCF

[Ohlsson et al., 2001]

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The Glass Ceiling

[Ohlsson et al., 2001]

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ChIP-chip reminder

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Breaking the Glass Ceiling

[Kim et al., 2007]

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A known locus

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More & less interesting correlations

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Outliers

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Consensus!

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Not all are captured

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From in vitro to in silico

[Xie et al., 2007]

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Genomic Distribution of Ultraconserved Elements

•exonic•non•possibly

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Uniquely Abundant in Coelacanth

?

x

Upto 80%id between Coelacanth instances

and some human instances, inc uc.338.

100 diverged copies in a Gigabase

60 highly similar copies in a Megabase

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Repeats / obile Elements ("selfish DNA")

HumanGenome:

3*109 letters1.5%

knownfunction >50%

junk

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The LF SINE (for Lobefin Fish / “Living Fossil”)

Reconstructionout back

target siteduplications

not similar to any known repeat

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To Be Continued….