UBC Engineering Physics UBC Applied Biophysics Laboratory
½ of these samples failed:
UBC Engineering Physics UBC Applied Biophysics Laboratory
Andre MarzialiApplied Biophysics Laboratory
Engineering PhysicsDepartment of Physics and Astronomy
University of British Columbia
Beyond CSIUltra-sensitive DNA analysis for
forensics and clinical diagnostics
UBC Engineering Physics UBC Applied Biophysics Laboratory
Copyright © 2005 BrightSide Technologies Inc.
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
Synchronous Coefficient of Drag Alteration: SCODA
UBC Engineering Physics UBC Applied Biophysics Laboratory
http://www.umich.edu/~morgroup/hsvm.html
λ phage DNA in gel
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
E field
E field
kEE += 0)( μμ
Evrr μ=
Ex
Ey
electrode
electrode
electrode
electrode
1 2
UBC Engineering Physics UBC Applied Biophysics Laboratory
ExEy
electrode
electrode
electrode
electrode
1 2
UBC Engineering Physics UBC Applied Biophysics Laboratory
Ex
Ey
electrode
electrode
electrode
electrode
1 2
UBC Engineering Physics UBC Applied Biophysics Laboratory
Ex
Ey
electrode
electrode
electrode
electrode
1 2
UBC Engineering Physics UBC Applied Biophysics Laboratory
)2cos(21
21)(cos2 ωω +=
Assume a quadratic velocity term
… and a rotating driving field:
( )2ˆ Ekv E=r
( )tEEx ωcos= ( )tEEy ωsin=
( )[ ] xx EtkEv d2cos2
d2cos
ωω=
⎥⎥⎦
⎤
⎢⎢⎣
⎡⎟⎟⎠
⎞⎜⎜⎝
⎛+⎟
⎟⎠
⎞⎜⎜⎝
⎛+= y
yxx
xx E
EEE
EE
EEkv ddd
2
UBC Engineering Physics UBC Applied Biophysics Laboratory
( )txEE qx ω2cosdd −= ( )tyEE qy ω2cosdd =
2cos2(x) = 1 + cos(2x) rv rr
4d
d qEkE−=
The quadratic term in the velocity generates a 2nd harmonic of ω:
So we beat this 2nd harmonic with a perturbing field at frequency 2ω:
Most molecules: DNA and RNA:
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
DNA Concentration
A. Marziali, J. Pel, D. Bizzotto, L. Whitehead, Electrophoresis 2005, 26, 82–90
1 cm
Genome BC Technology Development UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
Boreal Genomics
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
UBC Engineering Physics UBC Applied Biophysics Laboratory
Courtesy of Rob Holt, GSC
UBC Engineering Physics UBC Applied Biophysics Laboratory
1. Lysis 2. Loading
3. Electrophoresis 4. Concentrated DNAUBC Engineering Physics UBC Applied Biophysics Laboratory
Courtesy of Rob Holt, GSC
UBC Engineering Physics UBC Applied Biophysics LaboratoryUBC Engineering Physics UBC Applied Biophysics LaboratoryGenome BC Technology Development UBC Applied Biophysics Laboratory
P.S. I didn’t do much of the actual work….
http://http://www.physics.ubc.ca/~andrewww.physics.ubc.ca/~andre//
Collaborators:Lorne WhiteheadJulian DaviesRob Holt (BCCA–GSC)Vivian MiaoKaren LuHiron Poon (RCMP)
Vincent Tabard-CossaMatthew WigginCarolina TropiniDhruti TrivediNahid JethaGeorge SterlingChris Feehan
UBC Engineering Physics UBC Applied Biophysics Laboratory
But I did hire some excellent people that did:
David Broemeling Dylan Gunn Joel Pel Peter EugsterGiorgia Tropini Jason ThompsonLaura Mai … and many othersIvan ChanGareth Mercer
Boreal Genomics