uncompromised MS/MS simplicity Unstoppable productivity

Thermo Scientific TSQ 8000 Triple Quadrupole GC-MS/MS

2

your next competitive advantage...

Easy Operation, Brilliant Results • Uncompromised MS/MS simplicity

• Unstoppable productivity

• Automated SRM development

• Maximum uptime with vent-free source removal

The role of the analytical laboratory has changed in recent times as analytical data has become more commoditized. At the same time, competitive pressures have increased the demand for analytical cost savings while maintaining compliance with regulatory guidelines by producing high quality data with confidence on time, every time.

The Thermo Scientific TSQ 8000 triple quadrupole GC-MS/MS system is designed to provide the modern laboratory with unstoppable productivity and uncompromised MS/MS simplicity without sacrificing the high level performance you come to expect from Thermo Scientific mass spectrometers.

The TSQ™ 8000 GC-MS/MS is a system built for laboratories seeking a competitive edge to accelerate the turnaround time to get from raw sample to final report while reducing overhead costs. This GC-MS/MS system delivers high performance selected reaction monitoring (SRM) through the use of software that alleviates the usual concerns associated with the adoption of triple quadrupole GC-MS/MS technology.

3

4

unstoppable productivity Powerful MS/MS results, routinely

More Compounds, Less Time

The speed, sensitivity and selectivity delivered by the TSQ 8000 GC-MS/MS system allows more compounds to be run in shorter analysis times.

The structurally-selective nature of SRM reduces the likelihood of interference from matrix and other target compounds, allowing for the rapid analysis of more compounds in a single run. SRM has the ability to deliver clean peak detection along with automatic peak integration through Thermo Scientific TraceFinder software, and this solution enables the user to spend less time adjusting peak integrations while realizing faster result-reporting.

The Thermo Scientific ISQ single quadrupole GC-MS has a strong reputation in allowing laboratories to tackle the demands of producing results on time, every time. Building upon the success of this proven technology, the TSQ 8000 triple quadrupole GC-MS enables laboratories to take the next step in productivity through the power of selected reaction monitoring (SRM).

The compound selectivity advantage that SRM brings to GC-MS technology allows:

• Simple sample preparation

• Lower detection limits

• Faster analytical run times

Multiple single quadrupole pesticides methods consolidated into a single

40 minute 350 pesticides (700 transitions) run

• Consolidation of methods into a single analysis

• Faster data processing

• High confidence in compound identification and quantitation

Less Sample Prep, Faster Results

The selectivity of the TSQ 8000 GC-MS/MS system provided through SRM enables the ability to cut through heavy matrix background for clean peak detection and quantitation with confidence, in contrast to traditional single quadrupole GC-MS analysis. Therefore, costly sample preparation procedures can be optimized to deliver an efficient laboratory workflow.

Selectivity of SRM (bottom) vs

SIM (Top) for malathion at 5 ppb

in soil extract

5

More Robustness, More Conforming Results

The TSQ 8000 GC-MS/MS system combines high sensitivity with system robustness to ensure that your results stay within regulatory limits. Less maintenance intervention is needed, and the instrument can remain in production to report results for longer periods.

Precision at 10 pg/µL before and after 1,400 vegetable matrix injections

Timed SRM automatically optimizes

methods for maximum sensitivity

Less Method Maintenance, More Uptime

Retention-Timed SRM (t-SRM)

As more compounds are added to a single run, more complexity arises with the management of acquisition windows. The TSQ 8000 GC-MS/MS system dramatically reduces this complexity by automatically optimizing the targeting of a particular compound. Simply enter the retention time, and the time required to capture the peak and t-SRM takes care of the rest. This ensures that compound detection is optimized for maximum sensitivity, and more compounds can be added to the method without compromising the excellent sensitivity of each individual analyte. The mass spectrometer is not consuming valuable resources scanning for compounds at the times when those compounds are not eluting.

SR

M O

pti

miz

atio

nM

etho

d S

ync

MS/MS simplicity

6

The advantages of selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) analysis often come with challenges, especially with respect to adoption of new technology in the laboratory. These challenges are due to the complexity of mass spectrometer set-up and optimization and management of complex methods. Laboratories that strive to realize the benefits of MS/MS must overcome some barriers in the tuning, set-up and optimization to reduce the time required to achieve a smooth operating routine.

The TSQ 8000 GC-MS/MS was built to guarantee an easy start-up. Whether you are managing retention times, starting a completely new analysis, transferring a method from a single quadrupole, or porting a known MRM method from another instrument, the TSQ 8000 system ensures the fastest route to routine high performance results through its integrated software tools.

• AutoSRM: A purpose-built software for automated SRM method creation and optimization from full scan to the complete analytical method setup.

• TSQ 8000 GC-MS/MS Instrument Method: Offers true timed-SRM operation, allowing for high sensitivity and ease of use for the most complex SRM methods

• TraceFinder™ Software: State of the art, multi-platform user-friendly chromatography analysis software

AutoSRM

TSQ 8000 Instrument

Method

TraceFinder Software

From the Start

For new compounds, choose to begin the AutoSRM function, ask for a precursor ion study (Step 1), and place a vial in the autosampler. The AutoSRM function will report back to you a full scan chromatogram of the injection, and you simply identify your peaks and choose the most appropriate precursors that AutoSRM presents.

From an Existing SIM Method

If you are moving from a single quadrupole GC-MS method using SIM analysis, or you already know which ions you want to select for your precursor ions, you can start with Step 2. The AutoSRM function will perform product ion scans for each selected precursor and present the results in a single window. Simply select (or ask AutoSRM to select) the best product ion for that compound.

From a Previous MS/MS Method

If you have already performed previous AutoSRM steps or are simply moving from another MS/MS system, then Step 3 can take you to the final fully optimized SRM transitions for your compounds. Fully automated collision energy optimization is performed to ensure that transitions are as sensitive as they can be. These optimized SRM transitions are then exported into a finished MRM method ready for analyzing real samples in routine.

7

Step 1: Selects your precursor ions from full scan

Step 2: Selects your product ions from product ion scans

Step 3: Optimizes the collision energy for selected transitions

AutoSRM Workflow

Your Starting Point

Known SRM transitions• From an existing method

• From SRM transition list

Optimized SRM transitions• From Compound Data Store

• From existing TSQ 8000 GC-MS/MS method

Unknown SRM transitions• Start from the very beginning

• Start with existing SIM method

8

high performance in routine applications

For Contract Analysis, Food Safety, Environmental Monitoring, and Bioanalysis

The analytical advantages of high performance SRM are not limited to a single application area. Laboratories working in food safety, environmental monitoring and bioanalysis can all benefit from the unique advantages that the TSQ 8000 system brings. This is especially true for laboratories working in a production environment, such as performing contract analysis where delivering on-time, high quality, low cost analysis is high priority.

Take Environmental Analysis to the Next Level of Productivity

Triple quadrupole GC-MS/MS instruments are quickly becoming a competitive requirement for environmental laboratories. Method consolidation into large multi-analyte tests with generic sample preparation assist laboratories in the maintenance of low sample overhead. The TSQ 8000 system is the fastest route to productive triple quadrupole analysis available. It has been designed to ease the transition from single quadrupole methodologies and enable easy method and results management.

Confidence in Routine Forensic Toxicology Applications

Confident drug detection, whatever the matrix, is paramount even with high sample volumes. The high selectivity and robustness of the TSQ 8000 system means you can expect defendable and quality results every time you inject.

Multi-drug class screen in oral fluid. Cocaine and BZE at 0.8 ng/mL. THC and THCA at 0.2 ng/mL.

Quantitative SRM transitions on top row, confirmatory SRM transitions on bottom row.

1,3-Dichlorobenzene,

1,4-Dichlorobenzene

and 1,2-Dichlorobenzene

(peaks left to right) in

soil extract at 5 ppb

9

High-throughput in Pesticide Analysis

The TSQ 8000 system is well prepared for routine analysis of pesticides. It provides high robustness of the chromatographic system and MS ion source, thus reducing the need for frequent maintenance and avoiding system downtime for high sample throughput and productivity. The high matrix tolerance, sensitivity, and selectivity for target compounds allows for multi-residue confirmatory analysis to be performed. Clean detection and automatic integration of peaks results in faster data processing and result production.

Chemical Ionization Applications

Switching to a dedicated chemical ionization (CI) set-up is simple on the TSQ 8000 GC-MS. The Thermo Scientific ExtractaBrite ion source requires no venting of the MS system, and once in CI mode, the TSQ 8000 GC-MS enables quick and easy method setup for chemical ionization applications.

• The stages of instrument set-up and method development are all manageable through smart software tools integrated in the workflows needed for both positive and negative mode CI

• The TSQ 8000 automatically handles CI source tuning and optimization and offers automated switching between two CI gases

Peaks at 4 ppb from Tolylfluanid (left, 238.1 > 137.0, CE 15 V) and Pyridaben

(right, 309.1 > 147.1, CE 15 V) in lemon peel extract after 500 matrix injections

Quantitative performance at 10 ppb vegetable matrix for a selection of target pesticides and matrix calibration curves

BDE 209 at 2.5 ppb in negative chemical

ionization SRM mode

Aldrin: r2 0.9952

Endosulfan: r2 0.9994

Azinphos-Ethyl: r2 0.9976

Disulfoton: r2 0.9951

% RSD Ion Compound % RSD Ratio LOD95 (ppb)

Dichlooraniline 8.5 2.5 1.56

Azinphosethyl 4.6 1.0 0.84

Bifenazate 5.2 5.7 0.95

Bromopropylate 4.7 2.7 0.86

Carbaryl 5.0 1.2 0.92

Cyfluthrin 4.4 2.0 0.81

Deltamethrin 6.0 2.4 1.10

Endosulfan a 4.6 8.2 0.84

Etrimfos 3.8 4.5 0.70

Phosmet 4.9 1.4 0.90

Tecnazene 5.1 6.1 0.93

Tetraconazole 2.8 4.1 0.51

Vinchlozolin 3.3 7.4 0.60

• AutoSRM software can walk you through the CI method development process to obtain fully optimized SRM transitions

• Simultaneous negative and positive CI is possible with Pulsed-Positive Ion/Negative Ion Chemical Ionization (PPINICI)

10

powerful MS/MS; powerful MS

Add capability with GC-MS/MS, don’t compromise...

Single quadrupole (SQ) instruments have for years delivered excellent results in targeted and scanning determinations alike. Some regulations and methodologies still require the reporting of data based on the capabilities of single quadrupole analysis. Adding triple quadrupole technology enhances the capability of the laboratory. This is why the TSQ 8000 system was built to be a high performance instrument regardless of whether you wanted to perform MS or MS/MS experiments.

NIST ’08 Library search Pentachlorophenol >900 match

Pentachlorophenol full scan spectrum TSQ 8000

Hexachlorocyclopentadiene 100 ppb in

selected ion monitoring (SIM) mode

Simultaneous Acquisition Modes

11

Regulatory Tuning

The Target Tune function on a TSQ 8000 system ensures spectra created in a full scan analysis match “classic” single quadrupole GC-MS spectra. This facilitates comparison of spectra to standard libraries and makes it easy to meet regulations that specify excepted ion ratio ranges for certain compounds.

Retention Timed SIM (t-SIM)

Retention Timed SIM is available for the first time on the TSQ 8000 system. t-SIM makes complex SIM methods more efficient, more sensitive and easier to manage. Simply enter the mass and the retention time of your compound, and the TSQ 8000 GC-MS/MS takes care of the rest.

Offered in MS mode, as well as MS/MS, is the possibility to combine acquisition modes, such as SIM/full scan (and SRM/full scan) to understand the detail in each injected sample and identify unknowns by library search. Whether you monitor clean-up efficiency or catch an untargeted contaminant, a targeted-non-targeted approach can provide an edge without compromising the ability to measure low concentrations.

Classic spectra for DFTPP (Decafluorotriphenylphosphine) obtained using

Target Tune on a TSQ 8000 system

Lindane isomers 40 ppb in cucumber in simultaneous

full scan/SIM

12

brilliant design for brilliant results

Dual Filaments for Prolonged Uptime

Intelligent dual filament design keeps you running longer between maintenance intervals.

Dual Heated Zones on Source Maximize Ruggedness

Dual independently-heated zones on the TSQ 8000 system allow for thousands of injections of the dirtiest matrices before source cleaning is required.

Remove the Source of your Downtime

Allows full source removal without venting, the MS including ion guide surface. No wire connections are necessary, and venting is never necessary to clean your instrument.

No Noise is Good Noise

Off-axis optics provided by the curved ion guide effectively eliminate neutral noise, resulting in less background and lower limits of detection.

Never Clean or Replace Quads

Heated ion guide protects the main quadrupole sets so they never require cleaning or replacement.

Thermo Scientific

ExtractaBrite wireless ion

source cartridge

No Helium Required

Choose between nitrogen or argon for collision gas. No expensive helium is required.

Industry-Leading Detector Linearity

The Thermo Scientific DynaMax detection system standard on the TSQ 8000 system offers industry best linearity. Combined with the low detection limits attainable by SRM, this detector makes this mass spectrometer the ultimate quantitative instrument.

Fast Scanning Allows Fast Chromatography

Dynamic ion expulsion in the collision cell allows hundreds of MRM transitions per second. Combined with t-SRM for efficiently managed transition scheduling, the TSQ 8000 system can analyze hundreds of compounds with multiple transitions each over short chromatographic runs.

Switch Between Full Scan, SIM and MRM in Milliseconds

Effortless switching is made possible with the fast scanning capabilities of the TSQ 8000 GC-MS/MS. Pictured on the left is a simultaneous full scan/MRM acquisition of the dichlorobenzene isomers. On the right, a comparison of the 1,2-dichlorobenzene spectra obtained from the full scan/MRM acquisition (top) against the NIST full scan library spectra (bottom) is shown.

Off-Axis Optics

noise reducedto ~2 ions/sec

On-Axis (in-line) Optics

Noise reduction due to off-axis ion optics13

Maximum VersatilityThermo Scientific Xcalibur Data System

The Xcalibur™ data system is the core ‘operating system’ for each of the broad range of Thermo Scientific mass spectrometry systems. Offering a common set of easy-to-use, yet powerful, tools for GC/MS and LC/MS alike, Xcalibur software provides a unified experience for every user of every system. It offers instrument control, sample sequencing, and a set of programs for both qualitative and quantitative applications. Xcalibur software is also compatible with commercially-available mass spectral libraries, such as NIST, Wiley and Maurer-Pfleger-Weber,

and others. It also includes tools for generating and maintaining your own spectral libraries. Supporting each type of autosampler and GC available, Xcalibur software allows each mass spectrometer to be utilized to its fullest.

Easy Interpretation of Mass Spectral Data

Thermo Scientific Mass Frontier software provides a suite of options that allows the MS operator to interpret mass spectral data. This optional software package assists the operator in structural identification, isotope pattern determination, and spectral classifications.

Optimized ProductivityTraceFinder Software

The TraceFinder software application provides a streamlined workflow for the needs of a wide variety of high-throughput quantitative applications. Customized versions target the critical needs for key application areas, such as environmental, food safety, clinical research and forensic toxicology.

Beginning at the dashboard and working through each of the specialized modules, TraceFinder software guides the user through a highly productive workflow that is focused on the fastest route from sample to report.

Automated processing and QA/QC review, coupled to a reporting system capable of both standard and customized hardcopy and electronic deliverables, this software provides a comprehensive system for high-productivity quantitation.

14

intuitive software platforms for confident controlSolutions designed to meet the specific needs of your laboratory

TraceFinder software is designed to provide workflow guidance for those areas which benefit most from automation, while also providing sophisticated manual tools for those areas where the analyst’s expertise is key to completing that task at hand. From the very start, TraceFinder software is focused on efficient method development, rapid batch set-up, fluid data review, and flexible reporting.

Getting Started

Configuring the software to meet the unique needs of your laboratory is straightforward. Once set up, developing an optimized method is quick and easy through the use of Method Forge, an automated tool to take you from a standard data file to a complete method.

Getting Productive

Once the method is in place, the Batch Wizard accelerates the process of loading samples onto the system, targeting specific compounds, and selecting automated report options.

15

intuitive software platforms for confident controlSolutions designed to meet the specific needs of your laboratory

Getting Results

Following acquisition, automated processing kicks in, generating results sets for intuitive data review using a variety of customized views. Easy to see flags are available in the datasets, making it quick to determine which samples and compounds require attention based on concentrations, recoveries, ion ratios and a wide variety of user-defined metrics. On-the-fly modifications are immediately carried into the results, and no manual reprocessing is necessary. Once done, automated reporting can be started to generate print-outs and electronic files.

Data Review

Report View

Application Configuration

Batch View

streamlined, results-oriented workflow Software designed for the high-productivity laboratory

Method View

Active View

BR52289_E 09/12M

Africa-Other +27 11 570 1840Australia +61 3 9757 4300Austria +43 1 333 50 34 0Belgium +32 53 73 42 41Canada +1 800 530 8447China +86 10 8419 3588Denmark +45 70 23 62 60

Europe-Other +43 1 333 50 34 0Finland/Norway/Sweden +46 8 556 468 00France +33 1 60 92 48 00Germany +49 6103 408 1014India +91 22 6742 9434Italy +39 02 950 591

Japan +81 45 453 9100Latin America +1 561 688 8700Middle East +43 1 333 50 34 0Netherlands +31 76 579 55 55New Zealand +64 9 980 6700Russia/CIS +43 1 333 50 34 0South Africa +27 11 570 1840

Spain +34 914 845 965Switzerland +41 61 716 77 00UK +44 1442 233555USA +1 800 532 4752

www.thermoscientific.com©2012 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

Thermo Fisher Scientific, Austin, TX USA is ISO Certified.

excellence in GC-MS begins with the GC Match GC and autosampler options to your lab’s needs

Thermo Scientific chromatography columns and consumablesThe perfect partner for optimal analytical performance

Thermo Scientific chromatography columns and consumables are designed to complement our innovative range of GC and GC-MS systems. Get the most out of the TSQ 8000 GC-MS system by pairing it with advanced, high-performance Thermo Scientific products. The wide range of consumables and accessories offer customers applications-focused solutions in the environmental, food analysis, forensics/toxicology, petrochemical, pharmaceutical and general analytical industries.

Example of plug-in module installation by user

Ground-Breaking Plug-In ModularityWhy stop at a removable source design?

Tailor the Thermo Scientific TRACE 1300 Series GC to your needs with its proprie-tary user-exchangeable Instant Connect injector and detector modules. Swapping modules is easily done by the removal and replacement of three screws, accessible from the top of the GC. The entire process takes less than five minutes. This enables budget-conscious laboratories to purchase a base-configured GC today, then easily expand their capabilities to accommodate new applica tion and throughput demands.

Less is MoreThe oven, injectors, and detectors of the TRACE™ 1300 Series GC have a very low ther mal mass. This enables faster heat-up and cool-down times, reducing your inject-to-inject time and increasing your sample throughput.

Increase sensitivity with a completely new range of micro volume GC detectors. The unmatched sensitivity of these new instant connect detectors allows you to limit sample re-concentration requirements or reduce required initial sample amounts.

Easy Operation, Easy MaintenanceThe TRACE 1310 GC offers an intuitive, icon-based touch screen interface which allows for local control of the GC. In addition, tool-free access to septa and liners allows for quick routine maintenance. When more aggressive injector cleaning is needed, easy removal of the entire injector body allows for sonication in solvent. Finally, two easily accessible carbon traps ensure the injector and flow lines stay clean. Combined, these new features on the TRACE 1300 Series GC make it the most serviceable GC in the world.

Thermo Scientific AutosamplersPrecise sample introductions are required to achieve the best reproducibility and accuracy. The Thermo Scientific AI/AS 1310 autosampler and TriPlus™ RSH autosampler offer ideal solutions for automated injections.

TriPlus RSH Autosampler – Offers optimized liquid injection modes to support a wide range of sample types, inlets, and techniques for syringe-filling and injection.

AI/AS 1310 Autosampler – Offers a basic configuration for up to 8 vials at a time, while the AS 1310 holds 105 standard liquid autosampler vials. Excellent precision and tool-free alignment make this sampler a perfect choice for routine liquid sample injections.

TriPlus RSH Liquid Autosampler

With the TRACE 1300 Series GC paired with your TSQ 8000 GC-MS, tomorrow’s opportunity is in your hands today.