CURSO PRE Y POSTGRADO INTERNACIONAL

EVO-DEVO IN ASCIDIANS AND OTHER CLONAL MARINE DEUTEROSTOMES:

DEVELOPMENTAL GENES, STEM CELLS, REGENERATION

(PROYECTO SPRINT 4/2017- GRANT 2018/50017-0)

(FAPESP UNIV. SAO PAULO, USP-UNIVERSIDAD DE MAGALLANES, UMAG)

INTRODUCTION The biology of clonal organisms has been well studied in plants, protozoa and fungi, but less studied in animals. Although clonal propagation by fission and regenerative processes has received some attention, we believe that the study of budding processes and animal coloniality deserves more in depth study. In this class we want to bring together a group of interested and curious biologists that work on different topics to seek a synthesis and integration to understand the biology (physiology, ecology, development, etc.) and evolution (genetics of clonality, selection, etc.) of animal clonality and coloniality. We plan to contrast embryological developmental mechanisms of animals to post-embryonic/adult developmental processes of clonal and colonial deuterostomes. Using a theoretical-practical approach we will mainly focus on the study of fundamental aspects of life cycle evolution of common solitary and colonial marine chordates (i.e. ascidians), as well as some general aspects of larval cloning in echinoderms. We will discuss the evolutionary and biological consequences of clonality and colonial life histories in animals. Taking advantage of subantarctic fauna present in the Magellan region, we plan to explore the potential of local species to serve as laboratory models for the study of development and regeneration. Uses to these knowledgments in differents field of Biology research will be discussed.

GOALS

Train to young scientists with interest in Evo-Devo topics of deuterostomes

Acquire general knowledge and understanding of animal development, by a careful study and documentation of embryological and regenerative processes of local marina fauna. By the end of the course, students should be able to integrate developmental mechanisms in animals into an evolutionary framework, and to discuss current topics of interest in evo-devo.

o Familiarize with basic principles of comparative embryology and regenerative biology

Observe, identify morphological features, and visually represent the developmental stages of many invertebrates with different life histories.

Become familiar with techniques and experimental approaches used in evolutionary developmental biology studies.

Identify some practical uses of this basic Research.

ACADEMIC STAFF

1. Dr. Federico Brown A. (Universidad de Sao Paulo, Brasil) 2. Dra. Laurel Sky Hiebert (Post-Doc Universidad de Sao Paulo, Brasil)

3. Dr. Stefano Tiozzo (Lab. Biol. Development, Villefranche-sur-mer, CNRS, Universidad Pierre y Marie Curie, Francia)

4. Mag. Juan I. Cañete (Lab. Oceanografía Biologica Austral, LOBA, Facultad Ciencias, Universidad de Magallanes, Punta arenas, Chile)

LOCATION OF COURSE Facultad de Ciencias, Universidad de Magallanes, Punta Arenas, Chile, Av. Bulnes 01855, Punta Arenas, Chile, Lab. Biología, Primer Piso y Laboratorio CERESUB, Campus Instituto de la Patagonia, Av. Bulnes 01890, Punta Arenas, Chile.

DATE OF COURSE Lunes 9 al 12 diciembre, 2019.

DATE LINE OF APPLICATION Jueves 24 de noviembre, 2019. Forms and letter should be send in english, spanish, french

or portuguese.

DATE OF ACEPTATION OF APPLICATION Viernes 25 de noviembre, 2019

CERTIFICATION El curso será acreditado mediante un certificado de participación para quién hayan logrado una asistencia del 80%, cumplido con trabajos laboratorio y aprobado un test final. Se debe cancelar US$ 5 por el certificado tramitado por la Universidad de Magallanes.

DOCUMENTS TO APPLY:

1. Carta con expresión de interés en el curso 2. Curriculum vitae breve (máximo 3 páginas carta, letra times new roman 12) 3. Certificado alumno (a) regular actualizado 4. Formulario completo 5. Una carta recomendación de investigador en área de desarrollo organismos marinos.

COST OF COURSE El curso tendrá un costo de US$ 70/alumno (a). Alumnos de la USP o UMAG liberados de pago. El pago se efectuará al momento de la inscripción.

NUMBER OF STUDENTS Se estima un número adecuado de participantes de 25, entre alumnos de pre y postgrado.

ADDITIONAL INFORMATION OF PUNTA ARENAS CITY La región de Magallanes y Antártica Chilena está compuesta por cuatro provincias: Magallanes, Última Esperanza, Tierra del Fuego y Antártica, que en conjunto crean el territorio chileno continental y territorio Antártico, lo que la convierte en la región más extensa del país y puerta de entrada al continente blanco y las maravilla que este ofrece.

Debido a su gran extensión e influencia del relieve, el mar y los vientos, la región presenta importantes variaciones climáticas, que hacen que en un mismo día podamos tener lluvia,

sol, frío, calor o ráfagas de viento de hasta 100 km/hr. logrando tener marcadas estaciones del año, que regalan paisajes totalmente distintos con maravillosos colores, luces y sensaciones, todo el año.

Su naturaleza exótica ofrece además panoramas de gran diversidad, donde sus visitantes se pueden deslumbrar con la magia de la Cordillera Paine y el Campo de Hielo Patagónico Sur, sentir el poder de la montaña, o navegar por los canales de las islas australes, hasta alcanzar las rutas marítimas del Estrecho de Magallanes y sus canales, que conducen al Cabo de Hornos y la Antártica.

Aproximadamente el 65% del territorio de la región más extensa de Chile se encuentra protegida, resaltando el Parque Nacional Torres del Paine por sus singularidades geológicas, gran biodiversidad típica de la Patagonia chilena, el cual es una reserva de biosfera localizado a casi 500 km al norte de Punta Arenas.

El 2020 se celebran el V Centenario desde el descubrimiento del Estrecho de Magallanes por el navegante portugués Fernando de Magalhaes, hito turístico y marítimo que conecta el Océano Atlántico con el Oceánico Pacífico localizado sólo a 1000 m de la Universidad de Magallanes y desde donde es posible observar ballenas, delfines y una importante biodiversidad marina en la zona litoral urbana de Punta Arenas y visualizar la mítica Tierra del Fuego.

PROGRAMA CURSO EVO-DEVO IN ASCIDIANS AND OTHER CLONAL MARINE DEUTEROSTOMES:

DEVELOPMENTAL GENES, STEM CELLS, REGENERATION (PROYECTO SPRINT 4/2017- GRANT 2018/50017-0)

(FAPESP UNIV. SAO PAULO, USP-UNIVERSIDAD DE MAGALLANES, UMAG)

Day 1 (December 9th):

Lecture Room: - Presentations

- Course Briefings

- Mini-project explanations and topic selection (each student will select either a topic

provided by one of the instructors, or a topic of their interest)

- Introduction to Evo-Devo studies in Deuterostomes (Profs: J. Cañete/F. Brown)

Laboratory: - Lab procedures and preparation of tools (Profs: L. Hiebert)

- In vitro fertilization demo: Urchin (Profs: F. Brown)

- Regeneration demo: zooids, colonies, stolons (Profs: S. Tiozzo)

- Allorecognition demos: colonial ascidians (Profs: S. Tiozzo)

Day 2 (December 10th):

Lecture Room: - Development in deuterostomes (Profs: F. Brown/S. Tiozzo)

- Regeneration and larval cloning in deuterostomes (Profs: S. Tiozzo/L. Hiebert)

Laboratory: - In vitro fertilization demo: Ascidians (Profs: F. Brown)

- Regeneration: Larvae in Plankton (Profs: L. Hiebert)

Day 3 (December 11th):

Lecture Room: - Zooids allorecognition (Profs: S. Tiozzo/F. Brown)

- Life history evolution (Profs: L. Hiebert/J. Cañete)

- Tropical vs subtropical geographic patterns and life history (Profs: J. Cañete/L.

Hiebert)

Laboratory: - Observations and documentation for mini-projects

Day 4 (December 12th):

Laboratory: - Last observations and documentation for mini-projects

Lecture Room:- Final student presentations of mini-projects and discussion on practical applications of

these knowledgments

FORMULARIO INSCRIPCION CURSO

EVO-DEVO IN ASCIDIANS AND OTHER CLONAL MARINE

DEUTEROSTOMES: DEVELOPMENTAL GENES, STEM CELLS,

REGENERATION (PROYECTO SPRINT 4/2017- GRANT 2018/50017-0)

(FAPESP UNIV. SAO PAULO, USP-UNIVERSIDAD DE MAGALLANES, UMAG)

Complete Name

Passport number

Citizenship

Date of born

Professional Title

Academic degree

Career (students)

Name of posgraduate studies

Institution

Phone

Email

Field of work in Evo-Devo

Research

Letter with motivation for this

course

Curriculum Vitae

Letter of recomendation

SEND OF APPPLICATION TO:

Prof. Juan I. Cañete (UMAG-Chile)

Dpto. Ciencias y Recursos naturales, Facultad Ciencias, Universidad de Magallanes, Punta

Arenas, Chile. Fono: +56 612 209495 - 612-207938 (secretaria). Email: ivan.canete@umag.cl

Dr. Federico Brown (USP-Brasil)

Departamento de Zoologia, Instituto de Biociências USP, Rua do Matão. Travessa 14. no. 101,

Cidade Universitaria, São Paulo, SP, CEP 05508-090, Fono: +11-30910950

fdbrown@usp.br

Dr. Laurel Hiebert (USP-Brasil)

Departamento de Zoologia, Instituto de Biociências USP, Rua do Matão. Travessa 14. no. 101,

Cidade Universitaria, São Paulo, SP, CEP 05508-090, laurel@usp.br

Dr. Stefano Tiozzo (CNRS- France)

CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-Mer (LBDV), Sorbonne

Université, 06230 Villefranche-sur-Mer, France, tiozzo@obs-vlfr.fr

INFORMATION ON TEACHERS

PROF. JUAN I. CAÑETE Prof. Asociado, Biólogo marino, oceanógrafo y gestor ambiental. Su trabajo en la biología

del desarrollo se ha centrado en moluscos gasterópodos (Trochita), poliquetos plagas

(Spionidae), ascidias coloniales de la región de Magallanes (Polyzoa, Didemnum),

desarrollo larval de crustáceos de importancia económica y más recientemente ha

profundizado en caracterizar la componente larval del neuston de la zona austral de

Chile. En este último caso, se encuentran en ejecución proyectos para intentar

caracterizar el efecto de gradientes de salinidad sobre el desarrollo larval de una amplia

gama de invertebrados bentónicos solitarios y coloniales de la zona austral de Chile, un

estuario de 1700 km de extensión. El objetivo de esta investigación es definir la

importancia del neuston como bio-indicador del cambio climático en zonas templadas

frías sub antárticas.

Publicaciones 1. Cañete, J.I., J.L. López & E. Mutschke. 2019a. Ascidia ceratodes (Huntsman, 1912)

(Tunicata: Ascidiidae) off the northern Chilean coast: new record. Latin American

Journal of Aquatic Research 47: 184-189.

2. Schmidt-Rhaesa, A., M. Lee, M. Scherf, T. Figueroa, J. Sellanes & J. I. Cañete. 2019.

First report of marine Gastrotricha from Chilean beaches. Latin American Journal

of Aquatic Research 47: 684-693.

3. Cañete, J.I., C. S. Gallardo, A. Medina, J. Díaz-Ochoa, C. Olave & T. Figueroa.

2019. Abundance and spatial distribution of Polygordius (Polychaeta:

Polygordiidae) neustonic exolarvae in estuaries from southern Chile. Anales

Instituto de la Patagonia, 47: 19-33.

4. Cañete, J.I. 2018. Polyzoa opuntia (Ascidiacea) in the Magellan región, Chile: colony

shape changing with size and substrate. Anales Instituto Patagonia 46: 81-87.

5. Cañete, J.I. J. A. Díaz-Ochoa T. Figueroa & A. Medina. 2017. Infestation of

Pseudione tuberculata (Isopoda: Bopyridae) on juveniles of Lithodes santolla (region

of Magallanes, Chile): a spatial mesoscale analysis. Latin American Journal of

Aquatic Research 44: 576-587.

6. Cañete, J.I., C.S. Gallardo, C. Olave, M. S. Romero, T. Figueroa & D. Haro. 2016.

Abundance and spatial distribution of neustonic copepodites of Microsetella rosea

(Harpacticoida: Ectinosomatidae) along the western coast of Magellan, southern

Chile. Latin American Journal of Aquatic Research 44 (3): 576-587.

7. Turon, X., J I. Cañete, J. Sellanes, R. M. Rocha & S. López-Legentil. 2016. Too cold

for invasions? Contrasting patterns of native and introduced ascidians in

subantarctic and temperate Chile. Marine Biological Invasions 7: 77-86.

8. Turon, X., J.I. Cañete , J. Sellanes, R.M. Rocha & S.López-Legentil. 2016. Ascidian

fauna (Tunicata, Ascidiacea) of subantarctic and temperate regions of Chile.

Zootaxa 4093: 151-180.

9. Oyarzún, P., J. E. Toro, J.I. Cañete & J. P. A. Gardner. 2016. Bioinvasion threatens

the genetic integrity of native diversity and a natural hybrid zone: smooth-shelled

blue mussels (Mytilus spp.) in the Strait of Magellan. Biological Journal of the

Linnean Society 117: 574-585.

10. González-Wevar, C.A., P. Salinas, M. Hüne, N.I. Segovia, L. Vargas-Chacoff, M.

Astorga, J. I. Cañete & E. Poulin. 2015. Phylogeography in Galaxias maculatus

(Jenyns, 1848) along Two Biogeographical Provinces in the Chilean Coast. PlosOne

10(7): e0131289.

11. Cañete, J.I., C. Osorio & L. Huaquín. 2013-2015. Nuevos registros de imposex en

muricidos de la zona austral de Sudamérica (Estrecho de Magallanes). Revista

Ciencia y Tecnología del Mar 36: 81-90.

12. Cañete, J.I., C. A. Cárdenas, M. Palacios & R. Barría. 2013. Agregaciones

reproductivas pelágicas del poliqueto Platynereis australis (Schmarda, 1861)

(Nereididae) en zonas someras de canales fueguinos y magallánicos, Chile. Latin

American Journal of Aquatic Sciences 41: 170-176.

13. González-Wevar, C.A., A. Díaz, K. Gérard, J.I., Cañete & E. Poulin. 2013.

Divergence time estimations and contrasting patterns of genetic diversity between

Antarctic and southern South American benthic invertebrates. Revista Chilena de

Historia Natural 85: 445-456.

14. Cañete, J.I.. & R. M. Rocha 2013. Modiolarca lateralis (Bivalvia: Pteryomorphia:

Mytilidae) is a symbiont with six species of ascidians from Bocas del Toro, Panama.

Latin American Journal of Aquatic Sciences 41: 1030-1035.

15. Cañete, J.I. & V. Häussermann. 2012. Colonial life under the Humboldt Current

System: deep-sea corals from O’Higgins I seamount. Latin American Journal of

Aquatic Sciences 40: 467-472.

16. Cañete, J.I., C. S. Gallardo, T. Césped, C. A. Cárdenas & Mario Santana. 2012.

Encapsulated development, spawning and early veligers of the ranellid snail

Fusitriton magellanicus (Röding 1798) in the cold waters of the Magellan Strait,

Chile. Latin American Journal of Aquatic Sciences 40: 914-928.

17. Claudio A. González-Wevar, M. Hüne, J. I. Cañete, A. Mansilla, T. Nakano & E.

Poulin. 2012. Towards a model of postglacial biogeography in shallow marine

species along the Patagonian Province: lessons from the limpet Nacella magellanica

(Gmelin, 1791). BMC Evolutionary Biology Publicado online 7 agosto 2012.

Volumen 12.

18. Gallardo, C.S., D. Haro, C. Wagner, O. Garrido & J.I. Cañete. 2012. Egg laying

behavior and intracapsular development of Argobuccinum pustulosum (Gastropoda:

Ranellidae) in temperate waters at the South coast of Chile. Marine Biology

Research 8: 815-828.

19. González-Wevar, C.A., T. Nakano, J.I. Cañete & E. Poulin. 2011. Concerted genetic,

morphological and ecological diversification in Nacella limpets in the Magellan

Province. Molecular Ecology 20: 1936-1951.

20. Gonzalez-Wevar, C., M Nakano, JI Cañete & E. Poulin. 2010. Molecular phylogeny

and phylogeography in gastropods of genus Nacella (Patellogastropoda) from

Southern Ocean. Molecular Phylogenetics and Evolution. 56:

PROF. FEDERICO D. BROWN

Assistant Professor at the Universidade de São Paulo (USP). Leads the laboratory of

evolutionary developmental biology (EvoDevo Lab) in the Departmento de Zoologia of

the Instituto de Biociências (IB-USP). Research interests include: (1) evolution of

coloniality in marine cordates (i.e. ascidians); (2) stem cells and regeneration in

invertebrates; and (3) evolution of behaviors in nematodes.

Publicaciones 1. Juan Jiménez, Isadora Santos de Abreu, Silvana Allodi, Cintia M. de Barros, Stefano

Tiozzo, Federico D. Brown. Putative stem cells in the hemolymph and in the intestinal

submucosa of the solitary.ascidian Styela plicata. EvoDevo 2019 (accepted)

2. Hiebert LS, Vieira EA, Dias GM, Tiozzo S, Brown FD. (2019) Colonial ascidians

strongly preyed upon, yet dominate the substrate in a subtropical fouling community.

Proceedings of the Royal Society of London. Series B: Biological Sciences,

286:1899:20190396. DOI: 10.1098/rspb.2019.0396

3. Prünster MM, Ricci L, Brown FD, Tiozzo S. (2019) Modular co-option of

cardiopharyngeal genes during non-embryonic myogenesis. EvoDevo, 10:3. DOI:

10.1186/s13227-019-0116-7

4. Prünster MM, Ricci L, Brown FD, Tiozzo S. (2019) De novo neurogenesis in a budding

chordate: Co-option of larval anteroposterior patterning genes in a transitory

neurogenic organ. Developmental Biology, 448:2:342-52. DOI:

10.1016/j.ydbio.2018.10.009

5. Salamanca, D., Brown, F.D. (2018) Sub-functionalization of dorsal and ventral eyes

in a whirligig beetle (Coleoptera: Gyrinidae). Neotropical Biodiversity, 4:1:138-44.

DOI: 10.1080/23766808.2018.1510567

6. Alié A, Hiebert LS, Simion P, Scelzo M, Prünster MM, Lotito S, Delsuc F, Douzery

EJP, Dantec C, Lemaire P, Darras S, Kawamura K, Brown FD, Tiozzo S. (2018)

Convergent Acquisition of Nonembryonic Development in Styelid Ascidians.

Molecular Biology and Evolution. 35(7):1728-1743. doi: 10.1093/molbev/msy068

7. Velandia-Huerto CA, Brown FD, Gittenberger AA, Stadler PF, Bermudez-Santana

CI (2018) Non-protein-coding RNAs as regulators of development in tunicates. In

MARINE ORGANISMS AS MODEL SYSTEMS IN BIOLOGY AND MEDICINE.

Eds. M. Kloc & JZ Kubiak. Springer International Publishing. Pp. XII, 727. Series

65. eBook ISBN 978-3-319-92486-1, Hardcover ISBN 978-3-319-92485-4, Series ISSN

0080-1844, DOI 10.1007/978-3-319- 92486-1.

8. Gutierrez, S., Brown, F.D. (2017) Vascular budding in Symplegma brakenhielmi and

the evolution of coloniality in styelid ascidians. Developmental Biology, 423:152-69.

DOI: 10.1016/j.ydbio.2017.01.012

9. Chávez-Viteri, Y.E., Brown, F.D., Pérez, O.D. (2016) Deviating from the norm:

peculiarities of Aplysia californica early cleavage compared to traditional spiralian

models. Journal of Experimental Zoology – Part B, 328,1-2: 72-87. DOI:

10.1002/jez.b.22718

10. Velandia-Huerto, C.A., Gittenberger, A.A., Brown, F.D., Stadler, P.F., Bermudez-

Santana, C.I. (2016) Automated detection of ncRNAs in the draft genome sequence of

a basal chordate: The Carpet Sea Squirt Didemnum vexillum. BMC Genomics,

17:691. DOI: 10.1186/s12864-016-2934-5

11. Marcellini, S., González, F.A., Sarrazin, A.F., Pabón-Mora, N., Benítez, M., Pyñeyro-

Nelson, A., Rezende, G.L., Maldonado, E., Schneider, P.N., Grizante, M.B., Fonseca,

R.N., Vergara-Silva, F., Suaza-Gaviria, V., Zumajo-Cardona, C., Zattara. E.E.,

Casasa, S., Suárez-Baron, H., Brown. F.D. (2016) Evolutionary developmental biology

(Evo-Devo) research in Latin America. Journal of Experimental Zoology – Part B,

328, 1-2: 5-40. DOI: 10.1002/jez.b.22687

12. Quiroga, S. Y.*, Bonilla, E. C.*, Bolaños, D. M., Carbayo, F., Litvaitis, M. K., Brown,

F. D. (2015) Evolution of flatworm central nervous systems: insights from polyclads.

Genetics and Molecular Biology, 38, 3: 233-248. Doi: 10.1590/S1415-475738320150013

13. Moczek, A. P., Sears, K. E., Stollewerk, A., Wittkopp, P. J., Diggle, P., Dworkin, I.,

Ledon-Rettig, C., Matus, D. Q., Roth, S., Abouheif, E., Brown, F. D., Chiu, C.-H.,

Cohen, C. S., Tomaso, A. W. D., Gilbert, S. F., Hall, B., Love, A. C., Lyons, D. C.,

Sanger, T. J., Smith, J., Specht, C., Vallejo-Marin, M. and Extavour, C. G. (2015) The

significance and scope of evolutionary developmental biology: a vision for the 21st

century. Evolution & Development, 17: 198–219. doi: 10.1111/ede.12125

14. Stolfi, A., Brown, F. D. (2015) Book chapter: Tunicata. In EVOLUTIONARY

DEVELOPMENTAL BIOLOGY OF INVERTEBRATES 6: DEUTEROSTOMIA.

Editor Andreas Wanninger (University of Vienna, Austria). Springer, Wien. DOI

10.1007/978-3-7091-1856-6_4

15. Ogawa, A., Brown, F. D. (2015) Book Chapter: Dauer formation and dauer-specific

behaviours in Pristionchus pacificus. In PRISTIONCHUS PACIFICUS – A

NEMATODE MODEL FOR COMPARATIVE AND EVOLUTIONARY BIOLOGY.

Nematology Monographs & Perspectives. Vol. 11. Edited by Ralf Sommer (Max

Planck Institute for Developmental Biology, Tübingen, Germany). BRILL, Leiden-

Boston. Pp. 257-299.

16. Brown, F. D., Swalla, B. J. (2012) Evolution and development of budding by stem

cells: Ascidian coloniality as a case study. Developmental Biology. 369(2):151-62.

17. Brown, F. D.*, D’Anna, I.*, Sommer, R. (2011) Host finding behavior in the nematode

Pristionchus pacificus. Proceedings of the Royal Society of London. Series B:

Biological Sciences. 278 (1722): 3260-3269.

18. Brown, F. D.*, Tiozzo, S.*, Roux, M. M.*, Ishizuka, K., Swalla, B. J., De Tomaso,

A.W. (2009). Early lineage specification of long-lived germline precursors in the

colonial ascidian, Botryllus schlosseri. Development. 136: 3485-3494.

19. Brown, F.D.*, Keeling, E.*, Le, A., Swalla, B. J. (2009). Whole-body regeneration in

a colonial ascidian, Botrylloides violaceus. Journal of Experimental Zoology Part B:

Molecular and Developmental Evolution. 312: 885-900.

20. Brown, F. D., Prendergast, A., Swalla, B. J. (2008) Man is but a worm: chordate

origins. Genesis. 46:605-613.

21. Tiozzo, S., Voskoboynik, A., Brown, F. D., De Tomaso, A.W. (2008) A conserved role

of the VEGF pathway in angiogenesis of an ectodermally-derived vasculature.

Developmental Biology. 315 (1): 243-255.

22. Tiozzo, S., Brown, F. D., De Tomaso, A.W. (2008) Book chapter: Regeneration and

Stem Cells in Ascidians in STEM CELLS FROM HYDRA TO MAN. Ed. Thomas

Bosch. Springer. Pp. 95-112.

23. Brown, F. D., Swalla, B. J. (2007) Vasa expression in a colonial ascidian, Botrylloides

violaceus. Evolution & Development. 9: 165-177.

24. Brown, F. D., del Pino, E. M. (2003) Oogénesis y el órgano de Bidder en los machos

hermafroditas de Bufo marinus. Revista de la Pontificia Universidad Católica del

Ecuador. 71: 37-46.

25. del Pino, E. M., Pérez, O. D., Sáenz F. E., Brown, F. D., Ávila, M. E., Barragán, V. A.

(2003). Los polipéptidos asociados a la lámina nuclear 2 (LAP2) en 27 peces y anfibios.

Revista de la Pontificia Universidad Católica del Ecuador. 71: 27-35.

26. Brown, F. D., del Pino, E. M., Krohne, G. (2002) Bidder’s organ in the toad Bufo

marinus: Effects of orchidectomy on the morphology and expression of lamina-

associated polypeptide 2. Development, Growth and Differentiation. 44: 527-535.

27. del Pino, E. M., Sáenz, F. E., Pérez, O. D., Brown, F. D., Avila, M., Barragán, V. A.,

Haddad, N., Paulin-Levasseur, M., Krohne, G. (2002) Lamina-Associated Polypeptide

2 (LAP2) expression in fish and amphibians. International Journal of Developmental

Biology. 46: 227-234.

28. Brown, F. D. (2001) Oogénesis, el órgano de Bidder y patrón de expressión de

polipéptidos asociados a la lámina nuclear 2 (LAP2) en Bufo marinus

(ANURA:BUFONIDAE). Tesis de Licenciatura de la Pontificia Universidad Católica

del Ecuador. 1-135.

DR. LAUREL SKY HIEBERT

Postdoctoral researcher at the Universidade de São Paulo (USP). Member of the

laboratory of evolutionary developmental biology (EvoDevo Lab) in the Departmento

de Zoologia of the Instituto de Biociências (IB-USP). Research interests include: (1)

life cycle evolution and origin of morphological novelties in marine invertebrate life

histories; (2) evolution of coloniality, clonality, regeneration, and dormancy in

metazoans (focusing on tunicates) using eco-evo-devo approaches.

Publicaciones

1. Juan Jiménez, Isadora Santos de Abreu, Silvana Allodi, Cintia M. de Barros,

Stefano Tiozzo, Federico D. Brown. Putative stem cells in the hemolymph and in

the intestinal submucosa of the solitary.ascidian Styela plicata. EvoDevo 2019

(accepted)

2. Marta Scelzo, Alexandre Alié, Sophie Pagnotta, Camille Lejeune, Pauline Henry,

Laurent Gilletta, Laurel S. Hiebert, Francesco Mastrototaro, Stefano Tiozzo:

Novel budding mode in Polyandrocarpa zorritensis: A model for comparative

studies on asexual development and whole body regeneration. EvoDevo 12/2019;

10(1)., DOI:10.1186/s13227-019-0121-x

3. Laurel Sky Hiebert, Edson A Vieira, Gustavo M Dias, Stefano Tiozzo, Federico D

Brown: Colonial ascidians strongly preyed upon, yet dominate the substrate in a

subtropical fouling community. Proceedings of the Royal Society B: Biological

Sciences 03/2019; 286(1899):20190396., DOI:10.1098/rspb.2019.0396

4. Alexandre Alié, Laurel Sky Hiebert, Paul Simion, Marta Scelzo, Maria Mandela

Prünster, Sonia Lotito, Frédéric Delsuc, Emmanuel J. P. Douzery, Christelle

Dantec, Patrick Lemaire, Sébastien Darras, Kazuo Kawamura, Federico D.

Brown, Stefano Tiozzo: Convergent Acquisition of Nonembryonic Development in

Styelid Ascidians. Molecular Biology and Evolution 04/2018; 35(7).,

DOI:10.1093/molbev/msy068

5. Andrew Billings, Carl Kaiser, Craig M. Young, Laurel S. Hiebert, Eli Cole, Jamie

K.S. Wagner, Cindy Lee Van Dover: SyPRID Sampler: A Large-Volume,

High−Resolution, Autonomous, Deep-Ocean Precision Plankton Sampling System.

Deep Sea Research Part II Topical Studies in Oceanography 05/2016;,

DOI:10.1016/j.dsr2.2016.05.007

6. Cynthia D. Trowbridge, Laurel S. Hiebert, Juan Junoy, Colin Little, Penny

Stirling, Brittney Dlouhy-Massengale, Rob Mcallen: Nemerteans in an Irish

marine reserve: Synopsis of current and historical records. Marine Biodiversity

Records 10/2015; 8:e145., DOI:10.1017/S1755267215001189

7. Laurel S Hiebert, Svetlana A Maslakova: Expression of Hox, Cdx, and Six3/6 genes

in the hoplonemertean Pantinonemertes californiensis offers insight into the

evolution of maximally indirect development in the phylum Nemertea. EvoDevo

08/2015; 6(1):26., DOI:10.1186/s13227-015-0021-7

8. Laurel S Hiebert, Svetlana A Maslakova: Hox genes pattern the anterior-posterior

axis of the juvenile but not the larva in a maximally-indirect developing

invertebrate, Micrura alaskensis (Nemertea). BMC Biology 04/2015; 13(1):23.,

DOI:10.1186/s12915-015-0133-5

9. Terra C. Hiebert, George von Dassow, Laurel S. Hiebert, Svetlana Maslakova: The

peculiar nemertean larva pilidium recurvatum belongs to Riserius sp., a basal

heteronemertean that eats Carcinonemertes errans, a hoplonemertean parasite of

Dungeness crab. Invertebrate Biology 09/2013; 132(3):207-225.,

DOI:10.1111/ivb.12023

10. Minoree Kohwi, Laurel S Hiebert, Chris Q Doe: The pipsqueak-domain proteins

Distal antenna and Distal antenna-related restrict Hunchback neuroblast

expression and early-born neuronal identity. Development 03/2011; 138(9):1727-

35., DOI:10.1242/dev.061499

11. Laurel S. Hiebert, Greg Gavelis, George von Dassow, Svetlana A. Maslakova: Five

invaginations and shedding of the larval epidermis during development of the

hoplonemertean Pantinonemertes californiensis (Nemertea: Hoplonemertea).

Journal of Natural History 10/2010; 44(37-40-37-40):2331-2347.,

DOI:10.1080/00222933.2010.504893

DR. STEFANO TIOZZO

Research Director for National Center of the Scientific Research (CNRS) . Leads the

laboratory of evolutionary and developmental biology at the Villefranche sur Mer

Developmental Biology Laboratory (LBDV - Sorbonne University). Research interests

includes: (1) evolution of regeneration and non-embryonic developments, (2) stem cells

and regeneration in ascidians

1. Juan Jiménez, Isadora Santos de Abreu, Silvana Allodi, Cintia M. de Barros,

Stefano Tiozzo, Federico D. Brown. Putative stem cells in the hemolymph and in

the intestinal submucosa of the solitary.ascidian Styela plicata. EvoDevo 2019

(accepted)

2. Marta Scelzo, Alexandre Alie, Francesco Mastrototaro, Sonia Lotito, Federico

Brown, Stefano Tiozzo. Characterization of a novel mode of asexual development

in the colonial ascidian Polyandrocarpa zorritiensis. EvoDevo 2019 Apr 3;10:7.

doi: 10.1186/s13227-019-0121-x.

3. Laurel Sky Hiebert, Edson A. Vieira, Gustavo M. Dias, Stefano Tiozzo, Federico

D. Brown. Colonial ascidians strongly preyed upon, yet dominate the substrate in

a tropical fouling community. Proc Biol Sci. 2019 Mar 7;286(1899):20190396.

4. Maria Mandela Prünster, Lorenzo Ricci, Federico Brown, Stefano Tiozzo.

Modular co−option of cardiopharyngeal genes during non−embryonic myogenesis.

Evodevo. 2019 Mar 5;10:3. doi: 10.1186/s13227-019-0116-7. eCollection 2019.

5. Maria Mandela Prünster, Lorenzo Ricci, Federico Brown, Stefano Tiozzo. De

novo neurogenesis in a budding chordate: Co−option of larval anteroposterior

patterning genes in a transitory neurogenic organ. Dev. Biol. 2018 Oct 25. pii:

S0012-1606(17)30887-4. doi: 10.1016/j.ydbio.2018.10.009.

6. Alexandre Alié , Laurel S Hiebert, Paul Simion , Marta Scelzo, Maria Mandela

Prünster, Sonia Lotito, Frederic Delsuc , Emmanuel JP Douzery, Cristelle Dantec,

Patrick Lemaire , Sebastien Darras, Kazuo Kawamura, Federocp D Brown,

Stefano Tiozzo. Convergent Acquisition of Nonembryonic Development in Styelid

Ascidians. Mol Biol Evol. 2018 1;35(7):1728−1743. doi: 10.1093/molbev/msy068.

7. Lorenzo Ricci, Fabien Cabrera, Sonia Lotito, Stefano Tiozzo. Conservation of

germ layers specification domains during asexual and regenerative development.

Dev Biol. 2016 Aug 1;416(1):235-48.

8. Lorenzo Ricci, Ankita Chaurasia, Pascal Lapébie, Philippe Dru, Rebecca R.

Helm, Richard R. Copley, Stefano Tiozzo. Identification of differentially expressed

genes from multipotent epithelia at the onset of an asexual developmental. Sci Rep.

2016 Jun 6;6:27357.

9. Alberto Valero-Gracia, Rita Marino, Fabio Crocetta, Stefano Tiozzo, Paolo

Sordino. Evolution of serotoninergic neurotransmission in thaliaceans. Front Zool.

2016 Sep 29;13:45.

10. Rebecca R Helm, Stefano Tiozzo, Martin K S Lilley, Fabien Lombard and Casey

W Dunn. Comparative muscle development of jellyfish (Scyphozoa) with simple

and complex life cycles. EvoDevo. 2015 Apr 17;6:11

11. Alessandro Di Maio, Leah Setar, Anthony W. De Tomaso, Stefano Tiozzo. Wnt

affects symmetry and morphogenesis during post-embryonic development in

colonial chordates EvoDevo 2015 May 1;6:17

12. Stefano Tiozzo, Richard R. Copley. Reconsidering Regeneration in metazoans:

an evo-devo approach. Front. Ecol. Evol. 2015 June 3;67

13. Auxane Buresi, Roger P. Croll, Stefano Tiozzo, Laure Bonnaud, Sébastien

Baratte. Emergence of sensory structures in the developing epidermis in Sepia

officinalis and other coleoid cephalopods. J.Comp. Neur 2014 Feb 18.

14. Lucia Manni, Fabio Gasparini, Kohji Hotta, Katherine J. Ishizuka J, Lorenzo

Ricci, Stefano Tiozzo, Ayelet Voskoboynik, Delphine Dauga. Ontology for the

asexual development and anatomy of the colonial chordate Botryllus schlosseri

Plose ONE. 2014 May 1;9(5):e96434

15. Robert Lauzon, Catherine Castagna, Louis Kerr, Stefano Tiozzo. Phagocyte

dynamics in a highly regenerative urochordate: insights into development and host

defense Dev. Biol. 2013 374(2):357-73.

16. Stefano Tiozzo*, Kaz Kawamura*, Lucia Manni*, Takeshi Sunanaga*, Paolo

Burighel*, Anthony W. De Tomaso*. Gemline cell formation and gonad

regeneration in solitary and colonial ascidians. Dev. Dyn. 2011 240(2):299-308.

*equal contribution.

17. Stefano Tiozzo*, Ulrich Kuern*, Snjezana Rendulic*, Robert Lauzon. Asexual

propagation and regeneration in colonial ascidians. Biol. Bull. 2011 221(1):43-61

*equal contribution.

18. Annette Hellbach, Stefano Tiozzo, Jungho Ohn, Michael Liebling, Anthony W. De

Tomaso. Characterization of HCN2 and its cardiac function in the protochordate

B. schlosseri. J.Exp. Zool. A Ecol Genet Physiol 2011 315(8):476-86.

19. Stefano Tiozzo, Anthony W. De Tomaso. Functional analysis of Pitx during asexual

regeneration in a primitive chordate. Evol. Dev . 2009 11(2):152-62.

20. Stefano Tiozzo, Maureen Murray, Bernard M. Degnan, Anthony W. De Tomaso,

Roger P. Croll. Development of the neuromuscular system during asexual

propagation in an invertebrate chordate. Dev. Dyn. 2009 238(8):2081-94.

21. Stefano Tiozzo*, Federico D. Brown*, Michelle Roux*, Billie J. Swalla, Anthony

W. De Tomaso. Early lineage specification of long-lived germline precursors in the

colonial ascidian, Botryllus schlosseri. Development. 2009 136(20):3485-94. *equal

contribution.

22. Stefano Tiozzo, Federico D. Brown, Anthony W. De Tomaso. Regeneration and

Stem Cell in Ascidians. In “Stem Cells: From Hydra to Man”. 2008. Springer eds.

23. Stefano Tiozzo, Ayelet Voskoboynik, Federico D. Brown, Anthony W. De Tomaso.

A conserved role of the VEGF pathway in angiogenesis of an ectodermally-derived

vasculature. Dev. Bio. 2008 315(1):243-55.

24. Stefano Tiozzo, Loriano Ballarin, Paolo Burighel, Giovanna Zaniolo. Programmed

Cell Death In Vegetative Development: Apoptosis during the Colonial Life-Cycle

of the Ascidian Botryllus schlosseri. Tissue & Cell. 2006 38(3):193- 201

25. Stefano Tiozzo, Lionel Christiaen, Carole Deyts, Lucia Manni, Jean-Stephane

Joly, Paolo Burighel. Embryonic vs. Blastogenetic Development in the Compound

Ascidian Botryllus schlosseri: Insights from Pitx Expression Patterns. Dev. Dyn.

2005 232:469-479

26. Lucia Manni, Nancy J. Lane, Jean-Stephane Joly, Fabio Gasparini, Stefano

Tiozzo, Federico Caicci, Giovanna Zaniolo, Paolo Burighel. Neurogenic and Non

Neurogenic Placodes in Ascidians. J. Exp. Zool. Part B: Mol Dev Evol 2004 302(5):

483-504

27. Paolo Burighel, Nancy J. Lane, Fabio Gasparini, Stefano Tiozzo, Giovanna

Zaniolo, Lucia Manni. A Novel, Secondary Sensory Cell Organ In Ascidians: In

Search Of The Ancestor Of The Vertebrate Lateral Line J. Comp. Neurol. 2003

461:236-249

28. Marcello Cantini, Emanuele Giurisato, Claudia Radu, Stefano Tiozzo, Francesca

Pampinella, Doriana Senigaglia, Giovanna Zaniolo, Francesco Mazzoleni, Libero

Vitiello. Macrophage-Secreted Myogenic Factors: A Promising Tool for Greatly

Enhancing the Proliferative Capacity of Myoblasts in Vitro and in Vivo. Neurol.

Sci. 2002 23:189-194

Manuscripts submitted/under review

29. Kanoko Kishimoto, Alexandre Alie, Stefano Tiozzo, Kaz Kawamura.

Developmental role for Histone H3 lysine 4 demethylase in the establishment of

differentiation boundary of uni- and multipotent cells in budding tunicates (under

revision in Sci.Rep.)

30. Laurel Hiebert, Carl Simpson, Stefano Tiozzo. Coloniality, clonality and

modularity in animals: the elephant in the room. (under revision on JEZ part B)

31. Alexandre Alié , Laurel Hiebert, Marta Scelzo, Stefano Tiozzo. The eventful

history of non-embryonic development in colonial Tunicates. (Under revision in

JEZ part B).