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Use of dialysis to separate small and large molecules

Date post: 30-Dec-2015
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Use of dialysis to separate small and large molecules. Gel filtration chromatography. Molecular mass determination by gel filtration chromatography. Some commonly used gel filtration materials. Ion-exchange chromatography with stepwise elution. - PowerPoint PPT Presentation
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Use of dialysis to separate small and large molecules
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Page 1: Use of dialysis to separate small and large molecules

Use of dialysis to separate small and large molecules

Page 2: Use of dialysis to separate small and large molecules

Gel filtration chromatography

Page 3: Use of dialysis to separate small and large molecules

Molecular mass determination by gel filtration chromatography

Page 4: Use of dialysis to separate small and large molecules

Some commonly used gel filtration materials

Page 5: Use of dialysis to separate small and large molecules

Ion-exchange chromatography with stepwise elution

Page 6: Use of dialysis to separate small and large molecules

Device for generating a linear concentration gradient

Page 7: Use of dialysis to separate small and large molecules

Molecular formulas of cellulose-based ion exchangers

Page 8: Use of dialysis to separate small and large molecules

Some biochemically useful ion exchangers

Page 9: Use of dialysis to separate small and large molecules

Affinity chromatography

Page 10: Use of dialysis to separate small and large molecules

Covalent linking of ligand to agarose

Page 11: Use of dialysis to separate small and large molecules

Derivatization of epoxy-activated agarose

Page 12: Use of dialysis to separate small and large molecules

Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose

Page 13: Use of dialysis to separate small and large molecules

Experimental arrangement for paper chromatography

Page 14: Use of dialysis to separate small and large molecules

Two-dimensional paper chromatography

Page 15: Use of dialysis to separate small and large molecules

Paper electrophoresis

Page 16: Use of dialysis to separate small and large molecules

Apparatus for slab gel electrophoresis

Page 17: Use of dialysis to separate small and large molecules

Diagram of a disc electrophoresis apparatus

Page 18: Use of dialysis to separate small and large molecules

Polymerization of acrylamide and N,N-methylenebisacrylamide to form a cross-linked polyacryl-amide gel

Page 19: Use of dialysis to separate small and large molecules

SDS-PAGE

Page 20: Use of dialysis to separate small and large molecules

Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE

Page 21: Use of dialysis to separate small and large molecules

General formula of the ampholytes used in isoelectric focusing

Page 22: Use of dialysis to separate small and large molecules

Isoelectric points of several common proteins

Page 23: Use of dialysis to separate small and large molecules

Purification of rat liver glucokinase

Page 24: Use of dialysis to separate small and large molecules

END

Page 25: Use of dialysis to separate small and large molecules

An enzyme-linked immunosorbent assay (ELISA)

Page 26: Use of dialysis to separate small and large molecules

Solubilities of several proteins in ammonium sulfate solutions

Page 27: Use of dialysis to separate small and large molecules

Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type

Page 28: Use of dialysis to separate small and large molecules

Solubility of -lactoglobin as a function of pH at several NaCl concentrations

Page 29: Use of dialysis to separate small and large molecules

Protein crystals

Page 30: Use of dialysis to separate small and large molecules

Agarose gel electrophoretogram of a double helical DNA

Page 31: Use of dialysis to separate small and large molecules

Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs

Page 32: Use of dialysis to separate small and large molecules

Detection of proteins by immunoblotting

Page 33: Use of dialysis to separate small and large molecules

Two-dimensional (2D) gel electrophoresis

Page 34: Use of dialysis to separate small and large molecules

Sedimentation coefficients

in Svedbergs (S) for some

biological materials

Page 35: Use of dialysis to separate small and large molecules

A selection of preparative ultracentrifuge rotors

Page 36: Use of dialysis to separate small and large molecules

Zonal ultracentrifugation

Page 37: Use of dialysis to separate small and large molecules

Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm

Page 38: Use of dialysis to separate small and large molecules

Isopycnic ultracentrifugation

Page 39: Use of dialysis to separate small and large molecules

Physical constants of some proteins


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