UV_Vis

LAMBDA 25/35/45UV/Vis Spectrophotometers

keep your analysis flowing

WinLab software works the way you work, guiding youthrough a step-by-step process that simplifies analysisfrom sample entry to report generation, eliminatingcostly mistakes and delivering rapid results. You canrely on our proven technology to deliver consistentlyhigh-quality results over the lifetime of the system, andyou can confirm those results with our extensive suiteof Instrument Performance Verification (IPV) tests. ForFDA-regulated industries, the Enhanced Security (ES)version of UV WinLab integrates seamless 21 CFR Part11 technical compliance without slowing you down.

LAMBDA 25/35/45 UV/Vis Spectrophotometers in-crease productivity by mimicking the QA workflow togenerate high-quality results the first time, every time.And thats exactly what you need in a busy laboratorywhen the pressures on and its your job to keep theresults flowing. LAMBDA systems are easy to operateand deliver results you can trust with the minimum ofoperator training. With our complete range of LAMBDAsystems its easy to develop simple, robust methodsand ensure theyre followed without mistakes. And,whichever LAMBDA system you choose, your day-to-day UV/Vis analyses will be faster, simpler and moredependable than ever before.

speed and simplicity keep the results flowing

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The LAMBDA 25 is an idealmatch for most routine UV/VISapplications and regulatorytests, giving fast and reliableresults time after time.

The variable bandwidth of theLAMBDA 35 offers the bestsolution for measurements onsolids, pastes and powders.

The LAMBDA 45s premono-chromator deals easily withlight scattering samples.

LAMBDA 25 LAMBDA 35 LAMBDA 45

Range (nm) 1901100

Double-Beam Yes Yes YesOperation

Bandwidth (nm) 1 (fixed) 0.54 (variable) 0.54 (variable)

Pre-Monochromator No No Yes

Modes of Operation Scanning, Wavelength Program, Timedrive, Rate, Quant, Scanning Quant

Applications Routine UV/Vis testing Routine UV/Vis testing Routine UV/Vis testing

Liquids analysis Liquids analysis Liquids analysis

Pharmacopeia and Solids, pastes and Solids, pastes andregulatory tests powder samples powder samples

Regulatory tests requiring Regulatory tests requiringvariable bandwidths variable bandwidths

Highly scatteringsamples

www.perkinelmer.com 3

technologyfor best-in-class performance

Figure 1. LAMBDA systems exceed the pharmacopeia stray light testspecification of an absorbance of > 2.0A at 200 nm using a 1.2 w/vpotassium chloride solution. The additional pre-monochromator inthe LAMBDA 45 further reduces stray light compared to the LAMBDA 25 and 35.

With more than 10,000 units installed worldwide, oursuperior technology has proven itself under the toughestconditions.

True double-beam operation provides the best possiblestability and allows references to be measured andcorrected in real time.

Sealed and quartz-coated high-throughput opticsensure consistent performance throughout the instru-ments service life.

Figure 4. LAMBDA systems exceed pharmacopeia and ASTM re-quirements for wavelength accuracy (NIST 260-140) using a 4 mg/mlsolution of holmium perchlorate.

Figure 3. LAMBDA systems easily pass the pharmacopeia absorbanceaccuracy test at 235 nm, 257 nm, 313 nm and 350 nm using 60.06 mg/lpotassium dichromate solution.

Figure 2. Pharmacopeia resolution test using 0.02% w/v toluene inhexane at 1 nm slit width. Ratio of peak and trough near 269 nm and266 nm > 1.9 vs. the pass criterion of 1.5.

Specified Value (nm) Measured Value (nm) Maximum Tolerance (nm)

241.15 241.10 +/- 1.0

361.50 361.10 +/- 1.0

536.30 536.40 +/- 3.0

Fast scanning with no compromise in analyticalperformance.

Surpasses all regulatory body, quality system andpharmacopeia requirements.

Prealigned deuterium and tungsten halogen lampsmean that when a lamp needs to be replaced theres no service call and no downtime.

Figure 5. UV WinLab features a comprehensive list of IPV tests.

UV WinLab Enhanced Security

The Enhanced Security (ES) version of UV WinLab

software integrates 21 CFR Part 11 technical

compliance within the workflow user interface.

Unlike other packages with bolt-on compliance

modules, UV WinLab ES eliminates compliance

loopholes without compromising productivity.

Multi-level user permissions including

administrator, method developer, analyst

and reviewer.

Password-protected access control includes

password aging and expiry.

Method-lock facility ensures that methods

cannot be overwritten. All revisions are saved

in a single location.

Fully configurable e-signature points.

Six-level database security protects

valuable data.

Works the way you work

UV WinLab software mimics the way youwork, guiding you through method devel-opment, analysis, reporting and analysis ofresults in a series of simple steps so itseasy to keep in touch with all the stages ofyour analysis.

UV WinLab archives all of your results andmethods with a single mouse click in asecure, relational database. This transformsyour data from a collection of individual

UV WinLab software

results into valuable knowledge to help you make faster decisions.A range of intelligent querying options allows you to instantlyanswer questions from your customers and auditors and identifypotential problems before they happen.

Seamless validation and technical compliance

UV WinLabs integral performance tests give you the absoluteconfidence that your analyses and results will always meet thetoughest internal and external quality standards.

UV WinLab software includes a comprehensive suite of20 Instrument Performance Verification (IPV) tests (Figure 5)covering pharmacopeia, ASTM, GLP and ISO requirements.

Specify the intervals between IPV tests.

Compliance for FDA-regulated industries

As the leading supplier of UV/Vis systems to regulated industries,PerkinElmer is your partner for all stages on the route towardssystem validation. We provide a full suite of materials to help youcomplete a thorough validation plan according to the GAMP 4guidelines.

Detailed functional and system design specifications.

Materials to simplify preparation of your user requirementsspecification and supplier assessment questionnaire.

Manufacturing test specification and certificates.

Comprehensive IQ/OQ plan.

Data processing validation and suitability CD.

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sampling accessoriessimple, reproducible and robust

LAMBDA 25/35/45 systems offer the widest range of reproducible and robust sampling accessories to simplify and speed your analysis whatever your sample type. Choose the right accessory for your application at:www.perkinelmer.com/UVsupplies

The sensitive integrating sphere simplifies the analysis of a wide variety of sample types,including solids, powders, pastes and liquids.

A range of automatic linear cell changersspeeds and simplifies multi-sample measure-ments and quantitative calibrations.

A liquid autosampler is also available for high-throughput measurements using a wide rangeof sample vessels.

For everyday UV/Vis measurements, a widerange of routine cells and cell holders ex-tends the range of sample you can analyze.

The fiberoptic probeenables in-situ samplingaway from the instrument.

For liquids, the peristaltic sipperspeeds throughput.

Peltier controllers accuratelycontrol the temperature of yourexperiment.

The temperature measurement kit providesprecise in-cell temperature monitoring withreal-time display within UV WinLab.

Service, when and where you need it

OneSourceSM Laboratory Services from PerkinElmer is your worldwide source for comprehensive Service and Support. Our OneSource solutions are focused oncustomer value creation through improving instrumentperformance, increasing uptime/efficiency and driving

lab productivity. Whether you require customizedservice agreements, comprehensive validation services,or individualized expert training; OneSource LaboratoryServices has a solution to fit your needs, allowing youto focus on what matters most results.

mimics QA workflow for quick results

6

Simple method development

Scan, rate, quant, timedrive and wavelengthprogram modes provide maximum applica-tion flexibility. Method lock and passwordprotection keep key parameters from beingchanged, preventing errors. Fully integratedmath engine includes conditional formattingfor easy setup of pass and fail criteria. Savefrequently used equations as favorites. In theCommuniqu reporting environment, createcustomized report templates using a simpledrag-and-drop report editor.

Data Collection Setup

Sample Entry

Fast analysis

Purpose-designed sample entry tables in-clude information on sample preparation,sample description, supplier informationand control limits. Prompts for mandatoryinformation in sample table standardizeanalyses. GLP information is included in theheader and footer of the report. Reports caneven be automatically emailed anywheretheyre needed at the end of the analysis.

Easy access to your data

Secure, encrypted storage of all results in oneplace aids GLP and makes reviewing resultseasy. Intelligent querying allows searching onmultiple table parameters. Trending tools addanother dimension to your data review,analyze and predict.

Database Query


Data Processing Report Template

Results Report

Trending

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices

2004 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. LAMBDA, WinLab, Communiqu and EnhancedSecurity are trademarks and OneSource is a service mark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. All other trademarks not owned byPerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any timewithout notice and disclaims liability for editorial, pictorial or typographical errors.

006658B_01 Printed in USA

PerkinElmer Life and Analytical Sciences710 Bridgeport AvenueShelton, CT 06484-4794 USAPhone: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

PerkinElmer, Inc. is a global technology leader focusedin the following businesses Life and AnalyticalSciences, Optoelectronics, and Fluid Sciences. A $1 billion market leader, the Life and AnalyticalSciences division serves a large number of industries,including the academic research, biotechnology,clinical screening, pharmaceutical, environmental,forensic, petrochemical and semiconductor segments.

Our instruments and related software and customersupport programs, including our OneSourceSM teamof over 1,000 factory-trained service professionals

deployed in over 125 countries, help our customers toenhance research productivity, meet strict regulato-ry requirements, improve time-to-market, and increasemanufacturing efficiencies.

The LAMBDA 25/35/45 spectrophotometers are theUV/Vis systems of choice for thousands of laborato-ries worldwide. Discover for yourself how our proventechnology and excellent service and support willhelp to increase your labs productivity by deliveringconsistently high quality results over the entirelifetime of the systems.

LAMBDA UV/Vis/NIR and UV/Vis spectrophotometers:

powered by PerkinElmer

The PerkinElmer LAMBDA AdvantageINDUSTRY-LEADING INCREASED TECHNOLOGY POWER IN UV WINLAB PREMIUM SERVICE PERFORMANCE PRODUCTIVITY YOU CAN TRUST SOFTWARE GO-TO SUPPORT

For almost 50 years,PerkinElmer has been anindustry leader in UV/Vis/NIRand UV/Vis instrumentation,and continues to setbenchmarks with theinnovative LAMBDA Series.

Our workflow softwareand simple, robustsampling accessoriessimplify and speed youranalysis whatever your sample type.

With more than 10,000units installed worldwide,our superior technologyhas proven itself underthe toughest conditions.

Updated softwareworks the way you doby mimicking laboratoryworkflow and offeringthe tools and enhancedsecurity you need to meetall of your analyticalchallenges and datarequirements.

PerkinElmer is theonly major life andanalytical sciencesmanufacturer to havea dedicated servicebusiness OneSourceSMLaboratory Services.

LAMBDA UV/Vis and UV/Vis/NIR Spectrophotometers 850/950/1050

WHYLIMITYOURSELF?

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright 2007-2010, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 008021A_01 Printed in USA

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

Ask your account

manager for detailed

specification sheets.

Industry-leading Performance No Limits!

For more than 50 years, PerkinElmer has been an industry leader in spectrometry technology and continues to set the benchmark with the new LAMBDA 1050. Whether you need high-performing UV, Vis or NIR testing, the LAMBDA series wont limit you.

Lowest Sampling Setup Time

With intelligent design features like snap-in modules, large dual compartments and more, the LAMBDA series offers the industrys lowest sampling setup time.

Enhanced Flexibility and Convenience

The LAMBDA series offers multiple options and the versatility to handle the widest range of sample types. This flexible approach saves time, space and cost by combining all your testing capabilities into one system.

Intuitive UV WinLab Software

Updated software is easy to navigate and provides powerful analysis of your data. UV WinLab 6, with its Data Processor Viewer module, allows easy access and advanced data manipulation.

Global Service and Support

PerkinElmer is by your side today and tomorrow. With a global network of certified support professionals, were there to help maintain or service your instruments, and keep you up and running.

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices or visit our website at www.perkinelmer.com/new-limits.

The PerkinElmer LAMBDA Advantage

GET READY FOR RESULTS YOUVE NEVER SEEN BEFORE.

2

3LAMBDA Spectrophotometers

Your dreams arent limited, why should your capabilities be?

For more than 50 years, PerkinElmer has been setting the standard in spectrophotometry technology. Our instruments are known worldwide for data integrity, sampling flexibility and ease of use. Trendsetting features include snap-in modules, innovative accessories and intuitive software, all designed to make your process more efficient, versatile and productive.

We also know the importance of delivering reliable data on even the most demanding applications. Thats why with every instrument weve designed, weve advanced whats possible for your testing capabilities, whether its measuring the absolute reflectance of coatings at various angles with our LAMBDA 950 or analyzing highly absorbing liquids with the LAMBDA 850.

Now, with the new LAMBDA 1050, were pushing the limits even more. The LAMBDA 1050 redefines your range of capabilities by providing a better level of sensitivity, resolution and scanning speed in the NIR range than was previously possible. This opens up a whole new world of quality control, product improvement and discovery for countless industries and applications.

www.perkinelmer.com/new-limits

4LAMBDA 850

OPTIMAL PERFORMANCE IN THE UV/Vis

RANGE

For companies and products that only require UV/Vis test-ing capabilities, the LAMBDA 850 has just what you need. Equipped with our state-of-the-art gridless PMT detector,

the LAMBDA 850 provides excellent sensitivity between 175 and 900 nm, with resolution up to 0.05 nm. It also provides optimal sample control and flexibility with a range of features and accessories, including:

Large,dualsamplecompartments

UniversalReflectanceAccessory

Snap-inintegratingspheres

General-PurposeOpticalBench

Cosmetics and Sun Protection Products

Appearance and protection from UV rays are important considerationsinconsumerpurchases.Spectroscopic measurementsarecriticaltounderstandthingslikeSPF factors or determining the true color of a material.

Flat Panel Displays

The need to improve display performance continues in several areas. Color, brightness, viewing angle and power consumption are all important. Measurements are needed to improve overall performance.

Inks, Dyes, Pigments, Paints

With the explosion of digital photography, there is a need to develop inks and dyes that will represent true color and not fade over time. These products will require more accurate measurements.

Solar Energy Research

Silicon-basedsolarcellsarebecomingmoreimportantas a source of renewable energy. Once limited to use in space and military applications due to cost, research is ongoing to reduce cost and improve performance.

UV Vis

175 nm 900 nm

5LAMBDA 950

HIGH PERFORMANCE

ACROSS THE UV/Vis/NIR RANGE

Long the industry standard in spec-trophotometry, the LAMBDA 950 uses a gridless PMT with Peltier-controlled PbSdetectortoachieve high- performance testing across the spectral

range up to 3300 nm. The UV/Vis resolution reaches 0.05 nm, while the NIR resolution reaches up to 0.20 nm. It also features our industry-leading components and accessories for sample control and flexibility, including:

Large,dualsamplecompartments

UniversalReflectanceAccessory

Snap-inintegratingspheres

General-PurposeOpticalBench

Architectural and Specialty Glass

Energy conservation is more important now than ever before. Analysis of coated glass provides important information on thermal efficiency and other key design considerations.

Flat Panel Displays

The need to improve display performance continues in several areas. Color, brightness, viewing angle and power consumption are all important. Measurements are needed to improve overall performance.

Optical Component Measurements

Busy optical labs must be capable of dealing with a wide variety of components over a large number of measurement techniques. The flexibility of the LAMBDA series helps meet this ever-changing need.

Solar Energy Research

Solarcellsarebecomingmoreimportantasasourceofrenewable energy. Once limited to use in space and military applications due to cost, research is ongoing to reduce cost and improve performance. The LAMBDA 950s capability in the NIR region allows full characterization of not only the active solar cell materials but also the reflective protective covering.

UV Vis NIR

175 nm 3300 nm

www.perkinelmer.com/new-limits

6LAMBDA 1050

Responding to the need for a UV/Vis/NIR spectrophotometer with improved sensitivity, PerkinElmer is proud to offer the LAMBDA 1050. Now, you can achieve a greater level of sensitivity, resolution and

speed in the NIR range. Its perfect for a wide range of demanding applications, such as high absorbing glass, optical coatings or thin film filters. But the LAMBDA 1050s versatility doesnt end there. It also features two large sampling compartments and a variety of snap-in modules and accessories, including our unique, patented Universal Reflectance Accessory (URA), integrating spheres and a GeneralPurposeOpticalBench(GPOB).

Want a spectrophotometer that wont limit you today or tomorrow? The LAMBDA 1050 is the perfect choice.

OPTIMAL PERFORMANCE THAT TRULY GOES THE DISTANCE

UV Vis NIR

175 nm 3300 nm

7Smarter Flexibility

The LAMBDA 1050s smart modular design and range of snap-in accessories allow you to set up the instrument to suit a variety of needs and configurations, including:

Dual Sampling Compartment The LAMBDA 1050 can be configured with two large sampling compartments for twice the flexibility. Use one for standard reflectance or transmission tests, and the other for more specific customizable applications

Universal Reflectance Accessory Remove the standard detector and snap in this innovative accessory for automatically measuring sample angles

General-Purpose Optical Bench SwapinourGPOBtoaccommodate larger samples such as lenses, beamsplitters and optics

And the Fastest Setup Time

Withsomanyuser-friendlyfeatures,theLAMBDASeriesofferstheindustryslowestsamplingsetuptime.So,notonly can you go where youve never gone before, but you can get there the fastest way possible!

PerkinElmer also offers a standard validation kit for all of our LAMBDA spectrophotometers.

Unmatched Sensitivity and Resolution

TheLAMBDA1050featuresauniquePMT,InGaAsandPbS3-detector module for testing across the entire UV/Vis/NIR range.OurcooledInGaAsdetectorallowsyoutoextendthe spectral range up to 2500 nm. And because of its high sensitivity, you can set the resolution to the exact level you need, down to 0.2 nm.

Increased Scanning Speed

With its high sensitivity, the LAMBDA 1050 can scan faster than ever with no reduction in data quality.

Better Sample Control

The LAMBDA 1050 has been designed with a host of features and accessories to control your sample and ensure the quality of your data, including:

The industrys largest sample compartments for more sample maneuverability and quick installation of various accessories

Snap-in integrating spheres to capture diffuse and specular reflectance. Available in 60 and 150 mm, with 8 reflectance and center mount capability

Universal Reflectance AccessorywithdualSiandInGaAsdetectors for automated, precise and reproducible angle adjustments

Pol/Depol Drive Unit for automatic control of polarized or depolarized light via PC

Internal instrument controlled attenuation from 1 to 0.1%, for high absorbing measurements

The LAMBDA 1050 with dual compartments and a 150 mm snap-in integrating sphere.

8www.perkinelmer.com/new-limits

With the LAMBDA 1050s increased sensitivity in the

NIR range, you can handle a wide range of demanding

applications and measurements with more reliable results.

High Absorbing Glass

From cockpit and automotive windshields to laser protection shields to safety eyewear and headgear, the LAMBDA 1050 can look deeper, at higher resolutions with better signal-to-noise ratios. And the world can be safer because of it.

Optical Coatings

In the past, optical companies were limited when trying to measure coatings in the NIR range in order to improve the quality of their products. With the LAMBDA 1050, this has changed, and optical companies can finally get the competitive edge they need.

Fiber Optic Filters

For narrow band optical filters, such as DWDM filters, the LAMBDA 1050 can provide the necessary wavelength separation needed to ensure quality or detect areas of improvement. Once again, the LAMBDA 1050 answers the call for better testing methods.

Nanomaterials

The use of nanomaterials in optical, non-optical, renewable energy and advanced polymer design is on the rise. The wide array of sampling accessories and open architecture of the LAMBDA 1050 make it ideal for nanomaterial research and manufacturing testing needs.

For the most demanding applications

In just seconds, the LAMBDA 1050 can go from our standard large sample compartment instrument to operating with our 60 mm sphere, Universal Reflectance Accessory or General-Purpose Optical Bench.

9Only with LAMBDA

Interchangeable 3-detector Module

Sincemanycompaniestodayhaveawiderangeoftestingneeds,PerkinElmer developed a 3-detector module for the LAMBDA 1050 thatallowsforPMT,InGaAsandPbStestingallinoneinstrument. The 3-detector module provides ultimate testing flexibility, as well as optimal sensitivity, resolution and speed in the NIR range.

Universal Reflectance Accessory

A breakthrough in high-sensitivity, absolute reflectance measurements, our unique, patented Universal Reflectance Accessory (URA) dramatically improves on traditional methods of testing by automatically and reproducibly changing the angle of your sample. Previously, multi-angle analyses required several accessories and many manual adjustments. Now, sampling angles can be pre-set with a simple mouse-click, and the URA makes all the adjustments automatically. In addition, the sample is placed on a horizontal sampling plate to avoid damage caused by vertical clamping.

Two Large Sampling Compartments

Twice the flexibility. Twice the ease. All LAMBDA instruments can be configured to include dual sampling compartments, which also happen to be the largest in the industry. The primary compartment is used for a range of standard reflectance and transmission accessories and polarizers, while the second is customizable to a variety of intelligent sampling accessories or modules, including integrating spheres, the URA or transmission optics.

General-Purpose Optical Bench

Designed to accommodate large or oddly shaped optical samples, our GPOBgivesyoutheflexibilitytoconfigureameasurementinminutes.

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INSIDE THELAMBDA

11www.perkinelmer.com/new-limits

Another PerkinElmer Exclusive

All three of the LAMBDA series instruments

feature unique polarization measurement

capabilities to match your analytical needs.

Deuterium and Tungsten Halogen Light SourcesPrealigned and prefocused for quick replacement and maximum uptime. SourceDoublingMirror(LAMBDA1050only)forultra-high sensitivity.

Double Holographic Grating MonochromatorsFor ultra-low stray light performance.

Common Beam MaskAllows precise adjustment of beam height to match samples of different dimensions.

Common Beam DepolarizerCorrects for inherent instrument polarization to allow accurate measurements of birefringent samples (optional).

ChopperSwitchesbetweensampleandreferencebeam.Four-segmentdesign provides individual blank readings for sample and reference, increasing measurement accuracy.

Sampler and Reference Beam AttenuatorsFor extremely sensitive and accurate measurements on highly absorbing samples.

Largest Sample Compartment in the IndustryAllows easy access to a wide variety of sampling accessories and sample types.

High-sensitivity Photomultiplier and Peltier-controlled PbS DetectorsProvides full range UV/Vis/NIR coverage from 175 to 3300 nm (LAMBDA 950).

Second Sampling AreaHouses a range of snap-in sampling modules including transmission optics (shown), 60 mm and 150 mm integrating spheres and the Universal Reflectance Accessory for high-precision absolute reflectance measurements.

High-sensitivity Photomultiplier, Peltier-controlled InGaAs and PbS DetectorsProvides full range UV/Vis/NIR coverage from 175 to 3300 nm (LAMBDA 1050 only).

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Guides you from Setup to Results

PerkinElmers powerful UV WinLab software guides you through method development and reporting and analysis of results in simple steps. New UV WinLab 6 software includes an improved interface, easier access to stored data and a new data-viewing module.

ThesoftwareisalsoavailableinanEnhancedSecurity(ES)21CFRPart11compliantversionforregulatedindustries.

Ensuring Peak Performance Today and Tomorrow

With our worldwide network of certified technicians and service representatives, help is right around the corner for servicing or maintaining your laboratory instruments. Our global service capabilities include:

Preventivemaintenance

On-site,on-demandrepair

Softwareandhardwareupgrades

Validationservices

Trainingandconsulting

Freetechnicalandapplicationsphonesupport

UV WINLABSOFTWARE

SERVICE ANDSUPPORT

For the full range of PerkinElmer OneSource service and support offerings, visit us at www.perkinelmer.com/service.

GET READY FOR RESULTS YOUVE NEVER SEEN BEFORE.

2

LAMBDA UV/Vis and UV/Vis/NIR Spectrophotometers 850/950/1050

WHYLIMITYOURSELF?

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright 2007-2010, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 008021A_01 Printed in USA

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

Ask your account

manager for detailed

specification sheets.

Industry-leading Performance No Limits!

For more than 50 years, PerkinElmer has been an industry leader in spectrometry technology and continues to set the benchmark with the new LAMBDA 1050. Whether you need high-performing UV, Vis or NIR testing, the LAMBDA series wont limit you.

Lowest Sampling Setup Time

With intelligent design features like snap-in modules, large dual compartments and more, the LAMBDA series offers the industrys lowest sampling setup time.

Enhanced Flexibility and Convenience

The LAMBDA series offers multiple options and the versatility to handle the widest range of sample types. This flexible approach saves time, space and cost by combining all your testing capabilities into one system.

Intuitive UV WinLab Software

Updated software is easy to navigate and provides powerful analysis of your data. UV WinLab 6, with its Data Processor Viewer module, allows easy access and advanced data manipulation.

Global Service and Support

PerkinElmer is by your side today and tomorrow. With a global network of certified support professionals, were there to help maintain or service your instruments, and keep you up and running.

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices or visit our website at www.perkinelmer.com/new-limits.

The PerkinElmer LAMBDA Advantage

Technical Specifications for the LAMBDA 25/35/45UV/Vis Spectrophotometers

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/VIS

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www.perkinelmer.com

Technical description and specifications LAMBDA 25 LAMBDA 35 LAMBDA 45

Part number* (Standard System) L6020060 L6020064 L6020068

Part number* (Enhanced Security System) L6020062 L6020066 L6020070

Wavelength range 190-1100 nm 190-1100 nm 190-1100 nm

Bandwidth 1 nm fixed 0.5, 1, 2, 4 nm 0.5, 1, 2, 4 nmvariable variable

Stray light At 220 nm (NaI) < 0.01%T < 0.01%T < 0.005%TAt 340 nm (NaNO2) < 0.01%T < 0.01%T < 0.005%TAt 370 nm (NaNO2) < 0.01%T < 0.01%T < 0.005%TAt 200 nm (KCl) < 1%T < 1%T < 1%T

Wavelength At D2 peak (656.1 nm) 0.1 nm 0.1 nm 0.1 nmaccuracy

Wavelength 10 measurements at 656.1 nm 0.05 nm 0.05 nm 0.05 nmreproducibility

Photometric accuracy At 1 A using NIST 930D filter 0.001 A 0.001 A 0.001 AAt 2 A using NIST 1930D filter 0.005 A 0.005 A 0.005 A Potassium dichromate 0.010 A 0.010A 0.010 A

Photometric Maximum deviation ofreproducibility 10 measurements at 1 A < 0.001 A < 0.001 A < 0.001 A

Photometric stability Stability at 1 A, at 500 nm < 0.00015 < 0.00015 < 0.00015with 2-sec. response time A/hour A/hour A/hour

Photometric noise Noise 500 nm/0 A RMS < 0.00005 A < 0.00005 A < 0.00005 Aat 500 nm (RMS) Slit 1 nm

Baseline flatness Slit 1 nm 0.001 A 0.001 A 0.001 A


2004 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. LAMBDA is a trademark of PerkinElmer, Inc. or its subsidiaries,in the United States and other countries. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners.PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.

006658_02 Printed in USA


Technical description and specifications LAMBDA 25 LAMBDA 35 LAMBDA 45

Construction Solid CNC-machined aluminum chassis for thermal and vibration stability

Optics Double-beam, sealed, quartz-coated mirrors; lens-free system to reduce chromatic aberrations

Monochromator Seya Namioka

Grating Holographic, concave grating with 1053 lines per mm

Source Deuterium and Tungsten prealigned sources with automatic switch-over

Size (W x D x H) 650 mm (25 in.) x 560 mm (22 in.) x 233 mm (9 in.)

Weight (approx.) 26 kg (57 lbs)

The specifications listed above are confirmed and are based on final test pass criteria used in manufacturing.

Typical specifications may be superior to those listed.

*Requires PC

Introduction

PerkinElmer UV/Vis spectrophotometers are built to the highest ISO-9001 manufacturing standards. Thisdocument presents confirmed performance specifications based on factory tests. All instruments will meetor achieve better than the confirmed specifications, under normal conditions of use as described in theuser manual.

Technical Description and Specifications LAMBDA 650

Principle Double beam, double monochromator, ratio recording UV/Vis spectrophotometer with microcomputer electronics,controlled by DELL PC or compatible personal computer.

Optical System All reflecting optical system (SiO2 coated) with holographic grating monochromator with 1440 Lines/mm UV/Vis blazed at 240 nm,Littrow mounting, sample thickness compensated detector optics.

Beam Splitting System Chopper (46+ Hz, Cycle: Dark/Sample/Dark/Reference,Chopper Segment Signal Correction CSSC).

Detector R955 Photomultiplier, giving high energy throughout the whole UV/Vis range.

Source Pre-aligned tungsten-halogen and deuterium.

Wavelength Range 190 900 nm

UV/Vis Resolution 0.17 nm

Stray LightAt 200 nm (12 g/l KCl USP/DAP Method) > 2 AAt 220 nm (10 g/l NaI ASTM Method) 0.0001 %TAt 340 nm (50 mg/l NaNO2 ASTM Method) 0.0001 %TAt 370 nm (50 mg/l NaNO2 ASTM Method) 0.0001 %T

Wavelength Accuracy +/- 0.15 nm

Wavelength ReproducibilityDeuterium Lamp Lines 0.06 nm

Photometric AccuracyDouble Aperture Method 1 A +/- 0.0012 ADouble Aperture Method 0.3 A +/- 0.0006 ANIST 1930D Filters 2 A +/- 0.003 ANIST 930D Filters 1 A +/- 0.003 ANIST 930D Filters 0.5 A +/- 0.002 AK2Cr2O7 Solution USP/DAP Method +/- 0.01 A

Photometric Linearity(Addition of Filters at 546.1 nm, 2 nm Slit,1-sec. integration time) At 3.0 A +/- 0.02 A

Technical Specifications for the LAMBDA 650 UV/Vis Spectrophotometer

SP

EC

IF

IC

AT

IO

N

UV

/VIS

SP

EC

TR

OS

CO

PY

www.perkinelmer.com

Technical Description and Specifications (contd) LAMBDA 650

Photometric Reproducibility1 A with NIST 930D Filter at 546.1 nmStandard Deviation for 10 measurements 0.0008 A0.3 A with NIST 930D Filter at 546.1 nmStandard Deviation for 10 measurements 0.0004 A(2 nm Slit, 1-sec. integration time)

Photometric Range 6 A

Photometric Display Unlimited

Bandpass 0.17 nm5.00 nm in 0.01 nm incrementsUV/Vis rangeFix resolution, constant energy or slit programming.

Photometric StabilityAfter warm-up at 500 nm, 0 A, 2 nm Slit,2-sec. integration time, best-fit line 0.0003 A/h

Baseline Flatness200 nm 850 nm, 2 nm Slit, 0.2-sec.integration time, best-fit line +/- 0.0008 A

Photometric Noise RMS 0 A and 190 nm 0.00010 A0 A and 500 nm 0.00005 A2 A and 500 nm 0.00020 A4 A and 500 nm 0.002 A

(2 nm Slit, 1-sec. integration time)

Primary Sample Compartment Dimensions(W x D x H) 200 mm x 300 mm x 220 mm

Secondary Sample Compartment Dimensions(W x D x H) 480 mm x 300 mm x 220 mm

Instrument Weight 76 kg

Digital I/O RS 232 C

Instrument Dimensions 1020 mm x 630 mm x 300 mm

Light Beam 90 mm above the base plate120 mm beam separation3 mm12 mm beam height

Instrument RequirementsPower 90 VAC250 VAC, 50/60 Hz; 400 VATemperature 10C35CRecommended Humidity 1070% relative humidity, non-condensing


2004 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. LAMBDA is a trademark of PerkinElmer, Inc. or its subsidiaries,in the United States and other countries. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners.PerkinElmer reserves the right to change this document at any time and disclaims liability for editorial, pictorial or typographical errors.

006991A_15 Printed in USA


Introduction

PerkinElmer UV/Vis and UV/Vis/NIR spectrophotometers are builtto the highest ISO-9001 manufac-turing standards. This documentpresents confirmed performancespecifications based on factory tests.

All instruments will meet orachieve better than the confirmed

Technical Specifications for the LAMBDA 950 UV/Vis/NIR and LAMBDA 850 UV/Vis Spectrophotometers

SP

EC

IF

IC

AT

IO

N

UV

/VIS

AN

DU

V/V

IS/N

IRS

PE

CT

RO

SC

OP

Y

www.perkinelmer.com

specifications, under normalconditions of use as described in the user manual.

The LAMBDA Series of UV/Vis/NIR and UV/Vis spectrophotome-ters is the industry standard forhigh performance, flexibility andconvenience. Each model includes

Choose the LAMBDA 950 forultra-high UV/Vis/NIR performancefor wavelengths up to 3,300 nm, high-precision measurements, and forapplications such as highly reflectiveand anti-reflective coatings, colorcorrection coatings, bandpasscharacteristics of UV, Vis and NIRfilters, and more.

LAMBDA 850LAMBDA 950

Choose the LAMBDA 850 for ultra-highUV/Vis performance between 175 nm and900 nm. Sun protection, reflectanceproperties of flat panel display screens,paint films, transmission and reflectancecharacterization of glass, solar cells,and more.

175 nm 900 nm

UV-Vis

175 nm 3,300 nm

UV-Vis-NIR

the same range of modularcomponents and snap-in acces-sories to tackle a range of toughapplications. Whatever specifica-tions your laboratory requires,the LAMBDA Series providesbest-in-class accuracy, precisionand reproducibility.

Technical descriptionand specifications LAMBDA 950 LAMBDA 850

Principle Double beam, double monochromator, ratio Double beam, double monochromator,recording UV/Vis/NIR spectrophotometer ratio recording UV/Vis spectrophotometer with microcomputer electronics, controlled by with microcomputer electronics, controlled DELL PC or compatible personal computer. by DELL PC or compatible personal computer.

Optical System All reflecting optical system (SiO2 coated) All reflecting optical system (SiO2 coated) with holographic grating monochromator with with holographic grating monochromator 1440 Lines/mm UV/Vis blazed at 240 nm with 1440 Lines/mm UV/Vis blazed at 240 nm,and 360 Lines/mm NIR blazed at 1100 nm, Littrow mounting, sample thickness Littrow mounting, sample thickness compensated detector optics.compensated detector optics.

Beam Splitting System Chopper (46+ Hz, Cycle: Dark/Sample/Dark/ Chopper (46+ Hz, Cycle: Dark/Sample/Dark/Reference, Chopper Segment Signal Correction Reference, Chopper Segment Signal Correction CSSC). CSSC).

Detector Photomultiplier R6872 for high energy in the Photomultiplier R6872 for high energy in the whole UV/Vis wavelength range. Peltier cooled whole UV/Vis wavelength range.PbS detector for NIR.

Source Pre-aligned tungsten-halogen and deuterium. Pre-aligned tungsten-halogen and deuterium.Wavelength Range 175 nm3300 nm 175 nm900 nm(N2 purge required below 185 nm)

UV/Vis Resolution 0.05 nm 0.05 nmNIR Resolution 0.20 nm Stray Light At 200 nm (12 g/l KCl USP/DAP method) > 2 A > 2 AAt 220 nm (10 g/l NaI ASTM method) 0.00007 %T 0.00007 %TAt 340 nm (50 mg/l NaNO2 ASTM method) 0.00007 %T 0.00007 %TAt 370 nm (50 mg/l NaNO2 ASTM method) 0.00007 %T 0.00007 %TAt 1420 nm (H20 1-cm pathlength) 0.00040 %T At 1690 nm (CHCl3 4-cm pathlength) 0.0015 %T At 2365 nm (CHCl3 1-cm pathlength) 0.0005 %T

Wavelength Accuracy 0.08 nm UV/Vis 0.08 nm 0.30 nm NIR

Wavelength ReproducibilityUV/Vis (Deuterium Lamp Lines) 0.020 nm 0.020 nmNIR (Deuterium Lamp Lines) 0.080 nm Standard deviation of 10 measurements UV/Vis 0.005 nm 0.005 nmStandard deviation of 10 measurements NIR 0.020 nm

Photometric Accuracy Double Aperture Method 1 A 0.0006 A 0.0006 ADouble Aperture Method 0.5 A 0.0003 A 0.0003 ANIST 1930D Filters 2 A 0.003 A 0.003 ANIST 930D Filters 1 A 0.003 A 0.003 ANIST 930D Filters 0.5 A 0.002 A 0.002 AK2Cr2O7-Solution USP/DAP method 0.010 A 0.010 A

Photometric Linearity (Addition of filters UV/Vis at 546.1 nm, 2 nm Slit 1-sec. integration time)At 1.0 A 0.006 A 0.006 AAt 2.0 A 0.017 A 0.017 AAt 3.0A 0.020 A 0.020 A

2


Photometric Reproducibility1 A with NIST 930D Filter at 546.1 nmStandard Deviation for 10 measurements 0.00016 A 0.00016 A0.5 A with NIST 930D Filter at 546.1 nmStandard Deviation for 10 measurements 0.00008 A 0.00008 A0.3 A with NIST 930D Filter at 546.1 nmStandard Deviation for 10 measurements 0.00008 A 0.00008 A(2 nm Slit, 1-sec. integration time)

Photometric Range 8 A 8 APhotometric Display Unlimited UnlimitedBandpass 0.05 nm5.00 nm in 0.01 nm increments 0.05 nm5.00 nm in 0.01 nm increments

UV/Vis range UV/Vis range

0.20 nm20.00 nm in 0.04 nm increments Fixed resolution, constant energy or slit NIR range programming.

Fixed resolution, constant energy or slit programming.

Photometric StabilityAfter warm-up at 500 nm, 0 A, 2 nm Slit, 0.0002 A/h 0.0002 A/h2-sec. integration time, Peak to Peak

Baseline FlatnessLAMBDA 950: 190 nm3100 nm, Slit (UV/Vis) 2 nm, 0.0015 ASlit (NIR) Servo Mode, NIR Gain = 1,integration time 3100-860.8 nm : 0.24 sec.,integration time 860.8-190 nm : 0.20 sec.,no smoothing applied

LAMBDA 850: 190-860 nm, Slit 2 nm, 0.0015 Aintegration time 0.20 sec., no smoothing applied

Photometric Noise RMS 0 A and 190 nm 0.00010 A 0.00010 A0 A and 500 nm 0.00005 A 0.00005 A2 A and 500 nm 0.00020 A 0.00020 A4 A and 500 nm 0.00100 A 0.00100 A6 A and 500 nm 0.00500 A 0.00500 A0 A and 1500 nm 0.00004 A 2 A and 1500 nm 0.00100 A 3 A and 1500 nm 0.00300 A (2 nm Slit, 1-sec. integration time, Gain 1 NIR)

Primary Sample Compartment Dimensions (W x D x H) 200 mm x 300 mm x 220 mm 200 mm x 300 mm x 220 mm

Secondary Sample Compartment Dimensions(W x D x H) 480 mm x 300 mm x 220 mm 480 mm x 300 mm x 220 mm

PurgingOptics YES YESSample Compartment YES YES

Instrument Dimension (W x D x H) 1020 mm x 740 mm x 300 mm 1020 mm x 740 mm x 300 mmInstrument Weight ~ 77 kg ~ 77 kgDigital I/O RS 232 C RS 232 CLight Beam 90 mm above the base plate 90 mm above the base plate

120 mm beam separation 120 mm beam separation3 mm12 mm beam height 3 mm12 mm beam height

Instrument RequirementsPower 90 VAC250 VAC, 50/60 Hz; 400 VA 90 VAC250 VAC, 50/60 Hz; 400 VATemperature 10 C35 C 10 C35 CRecommended Humidity 1070% relative humidity, non-condensing 1070% relative humidity, non-condensing

3www.perkinelmer.com


2005 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks and LAMBDA is a trademark of PerkinElmer, Inc. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time and disclaimsliability for editorial, pictorial or typographical errors.

006991B_12 Printed in USA


Introduction

PerkinElmer UV/Vis and UV/Vis/NIR spectrophotometers are built to the highest ISO-9001 manufac-turing standards. This document presents confirmed performance specifications based on factory tests.

All instruments will meet or achieve better than the confirmed specifications, under normal conditionsof use as described in the user manual.

The LAMBDA Series of spectrophotometers is the industry standard for high performance, flexibilityand convenience. Each model includes the same range of modular components and snap-in accessoriesto tackle a range of tough applications. Whatever specifications you require, the LAMBDA Seriesprovides best-in-class accuracy, precision and reproducibility.


Principle Double beam, double monochromator, ratio recording UV/Vis/NIRspectrophotometers with microcomputer electronics, controlled byDELL PC or compatible personal computer.

Technical Specifications for theLAMBDA 1050 UV/Vis/NIR andLAMBDA 950 UV/Vis/NIRSpectrophotometers

SP

EC

IF

IC

AT

IO

NS

UV/V

ISAND

UV/V

IS/N

IRSPECTROSCOPY

www.perkinelmer.com

Choose the LAMBDA 950 for ultra-high UV/Vis/NIRperformance for wavelengths up to 3300 nm, high-precision measurements, and for applications suchas highly reflective and anti-reflective coatings, colorcorrection coatings, bandpass characteristics of UV,Vis and NIR filters, and more.

LAMBDA 950

175 nm 3300 nm

UV-Vis-NIR

Choose the LAMBDA 1050 with its triple detectorcapability for ultra-high UV/Vis/NIR performance forwavelengths up to 3300 nm with extreme sensitivityin the NIR region (800-2500 nm) and higher energythroughput. For applications such as highly reflectiveand anti-reflective coatings, all types of glass fromclear to highly absorbing safety glass, optical filtersof all types from the deep UV through the NIR andmany more applications requiring the best photo-dynamic range and the widest possible samplingcapability.

LAMBDA 1050

175 nm 3300 nm

UV-Vis-NIR


Optical System All reflecting optical system (SiO2 coated) with holographic gratingmonochromator with 1440 lines/mm UV/Vis blazed at 240 nm and 360 lines/mm NIRblazed at 1100 nm, Littrow mounting, sample thickness compensated detector optics.

Beam Splitting System Chopper (46+ Hz, Cycle: Dark/Sample/ Dark/Reference, Chopper Segment SignalCorrection).

Detector Photomultiplier R6872 for high energy Photomultiplier R6872 for high energy in thein the entire UV/Vis wavelength range. entire UV/Vis wavelength range. High per-Combination of high performance formance Peltier-cooled PbS detector forPeltier-cooled InGaAs detector, 2 options: the NIR wavelength range.Narrow band covering 860-1800 or wideband covering 860-2500 nm and Peltier-cooled PbS detector for 1800/2500-3300nm in the NIR wavelength range.

Source Pre-aligned tungsten-halogen and Pre-aligned tungsten-halogen and deuterium.deuterium. Utilizes a source doublingmirror for improved UV/Vis/NIR energy.

Wavelength RangeN2 purge required below185 nm 175 nm - 3300 nm 175 nm - 3300 nm

UV/Vis Resolution 0.05 nm 0.05 nm

NIR Resolution 0.20 nm 0.20 nm

Stray LightAt 200 nm12 g/l KCl USP/DAP method > 2 A > 2 AAt 220 nm10 g/l NaI ASTM method 0.00007 %T 0.00007 %TAt 340 nm50 mg/l NaNO2 ASTM method 0.00007 %T 0.00007 %TAt 370 nm50 mg/l NaNO2 ASTM method 0.00007 %T 0.00007 %TAt 1420 nmH20 1 cm path length 0.00040 %T 0.00040 %TAt 2365 nmCHCl3 1 cm path length 0.00050 %T 0.00050 %T

Wavelength AccuracyUV/Vis 0.080 nm 0.080 nmNIR 0.300 nm 0.300 nm

Wavelength ReproducibilityUV/Vis (Deuterium lamp lines) 0.010 nm 0.020 nmNIR (Deuterium lamp lines) 0.040 nm 0.080 nm

Standard deviation of10 measurements UV/Vis 0.005 nm 0.005 nmStandard deviation of10 measurements NIR 0.020 nm 0.020 nm

2


Photometric AccuracyDouble Aperture Method 1 A 0.0003 A 0.0006 ADouble Aperture Method 0.5 A 0.0003 A 0.0003 ANIST 1930D Filters 2 A 0.0030 A 0.0030 ANIST 930D Filters 1 A 0.0030 A 0.0030 ANIST 930D Filters 0.5 A 0.0020 A 0.0020 AK2Cr2O7-Solution USP/DAPmethod 0.0080 A 0.0080 A

Photometric LinearityAddition of filters UV/Vis at546.1 nm, 2 nm slit, 1 secondintegration timeAt 1.0 A 0.0060 A 0.0060 AAt 2.0 A 0.0160 A 0.0170 AAt 3.0 A 0.0050 A 0.0200 ANIR At 1.0 A (1200 nm) 0.0005 ANIR At 2.0 A (1200 nm) 0.0010 A

Photometric ReproducibilityStandard deviation for 10measurements, 2 nm slit,1 second integration time1 A with NIST 930D Filterat 546.1 nm 0.00016 A 0.00016 A0.5 A with NIST 930D Filterat 546.1 nm 0.00008 A 0.00008 A0.3 A with NIST 930D Filterat 546.1 nm 0.00008 A 0.00008 A

Photometric RangeUV/Vis 8 A 8 ANIR 8 A 6 A

Photometric Display Unlimited Unlimited

Bandpass 0.05 nm - 5.00 nm in 0.01 nm increments UV/Vis range

0.20 nm - 20.00 nm in 0.04 nm increments NIR range

Fixed resolution, constant energy or slit programming.

Photometric StabilityAfter warm-up at 500 nm, 0 A,2 nm slit, 2 second integrationtime, peak to peak 0.0002 A/h 0.0002 A/h

Baseline Flatness190 nm - 3100 nm, 2 nm slit0.20 second integration timeUV/Vis, no smoothing applied 0.0008 A 0.0008 A0.24 second integration timeNIR, no smoothing applied

3www.perkinelmer.com


Photometric Noise RMS2 nm slit, 1 second integration time0 A and 190 nm 0.00010 A 0.00010 A0 A and 500 nm 0.00005 A 0.00005 A2 A and 500 nm 0.00020 A 0.00020 A4 A and 500 nm 0.00100 A 0.00100 A6 A and 500 nm 0.00500 A 0.00500 A0 A and 1500 nm 0.00002 A 0.00004 A2 A and 1500 nm 0.00010 A 0.00010 A3 A and 1500 nm,PbS (Servo) 0.00250 A 0.00300 A0 A and 1500 nm InGaAs 0.00002 A2 A and 1500 nm InGaAs 0.00010 A3 A and 1500 nm,Wide Band InGaAs (Servo) 0.00010 A3 A 1500 nm,Narrow Band InGaAs (Servo) 0.000025 A

Primary Sample CompartmentDimensions (W x D x H) 200 mm x 300 mm x 220 mm 200 mm x 300 mm x 220 mm

Secondary Sample CompartmentDimensions (W x D x H) 480 mm x 300 mm x 220 mm 480 mm x 300 mm x 220 mm

PurgingOptics YES YESSample Compartment YES YES

Instrument Dimension(W x D x H) 1020 mm x 740 mm x 300 mm 1020 mm x 740 mm x 300 mm

Instrument Weight ~ 77 kg ~ 77 kg

Digital I/O RS 232 C RS 232 C

Light Beam 90 mm above the base plate, 120 mm beam separation, 3 mm - 12 mm beam height

Instrument RequirementsPower 90 VAC - 250 VAC, 50/60 Hz; 250 VA 90 VAC - 250 VAC, 50/60 Hz; 250 VATemperature 10 C - 35 C 10 C - 35 CRecommended Humidity 10-70% relative humidity, 10-70% relative humidity,

non-condensing non-condensing


2007 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. PerkinElmer is a registered trademark and LAMBDA is atrademark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. DELL is a trademark of DELL, Inc. All other trademarks not owned by PerkinElmer, Inc. or itssubsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liabilityfor editorial, pictorial or typographical errors.

008021_02 Printed in USA

PerkinElmer, Inc.940 Winter StreetWaltham, MA 02451 USAPhone: (800) 762-4000 or(+1) 203-925-4602www.perkinelmer.com

Its not just a UV/Vis.Its a LAMBDA.

The Bio-calculator with the heart of a LAMBDA.

Introducing the PerkinElmer LAMBDA Bio and Bio+ personal UV/Vis spectrophotometers for allyour routine life-science calculations. Continuing on our tradition of providing you with quality UV/Visinstruments for more than 60 years, these low-cost spectrophotometers have been designed to acquireand calculate results in a matter of seconds. With the same complexity as a desktop calculator, theLAMBDA Bio and Bio+ have a series of pre-programmed methods to support the demands ofboth molecular biology and biotech laboratories:

DNA, RNA, concentration and purity, and Oligonucleotide methods Protein methods including: Direct UV, BCA, Bradford, Lowry, Buiret and Cell density measurements Curve fitting with up to 9 standards in triplicate assures accurate results

Performance that wows the crowd and the finance people.With its large LCD screen and full scanning capabilities, the LAMBDA Bio is a crowd pleaser.Built to last, easy to use and even easier to own. No moving parts, virtually no maintenance andno consumables make the LAMBDA Bio the perfect tool when the instrument just needs to work,day-in and day-out. Rely on the LAMBDA Bio. Its the smart choice for your laboratory in anyapplication from teaching where operator training may be an issue, to the most advanced R&Dlaboratory where precious samples need to be processed right, the first time. The LAMBDA Biowill win the hearts and minds of anyone working with it. After all, it is a LAMBDA.

LAMBDA Bio and Bio+Spectrophotometers

Speed, stability and confidence!Order online at www.perkinelmer.com/lambdabio

You dont need an impressive budget,to have an impressive instrumentThe performance youll experience with the LAMBDABio and Bio+ is only normally associated with muchmore expensive designs. Experience:

Instant answers unlike other instruments on themarket, our LAMBDA Bio requires no warm up time.Our ultra fast initialization will allow you to capturea full range of spectrum in less than 3 seconds.

Continuous uptime our maintenance-free designwith no moving parts and Xenon light source pro-vides you with the reliability your laboratory needs.

Superb stability avoid instrument drift from excessheat with our split beam design.

Ruggedness our sealed, zero bounce membranekeypad is resistant to even the largest solvent spills.

Provides you with the versatility to dojust about anythingYour work environment may be complex but thatdoesnt mean your instrument needs to be. With thesimplicity and elegance of the LAMBDA Bio, youll beempowered to analyze samples when and whereyour projects demand:

View, expand, and manipulate full spectra quicklyand easily with the clear 11.5 cm by 8.7 cm backlitLCD display.

Feel confident low stray light provides exceptionallinearity and confidence in your measurements.

Keep up the pace our innovative polychromatortechnology will give you the speed and performanceyou need to keep up with your fast paced laboratory.

Save time our optical design allows samples to berun with no sample compartment lid.

Personalize your laboratory the huge on-boardmemory storage will allow you to store up to 90methods along with unique method names andcalibration information (only available on the Bio+).

Convenient sampling flexibility measure yoursamples in any of our high quality precision glass,quartz, or disposable cells:

Macro cells: 3.5 ml

Semi-micro cells: 1.4 ml

Micro cells: 750 l

Ultra-micro cells: 15 l

2

designed to make

spectrophotometry easier

Share the knowledge multiplearchiving and data sharing options

Collect data from up to 8 instruments:a single PC can be configured to accept and auto-matically display data from up to 8 instruments.Data can be archived in Excel (.xls), ASCII (.csvor .txt), graphics (.emf), or rich text (.rtf) formats.

Built-in USB port: transfer data to your computerfor viewing, manipulating, printing, or archiving usingthe built in USB connection port. LAMBDA Bio PVCsoftware provided as standard with all instruments.

Optional integrated thermal printer: Foryour permanent records, use the optional thermalprinter to print all your results. With the standard57 mm printer roll, your print-out can accommodate:

Calibration Data

Result

Time and Date

Secure Digital (SD) Flash Storage Cardor Bluetooth compatible: for your archivingconvenience, the LAMBDA Bio can be outfittedto accommodate a standard SD flash storage cardor Bluetooth accessory allowing you additionalflexibility for transferring data.

Experience the power flexibleon-board software

The LAMBDA Bio software is optimized to provideyou with answers fast and conveniently. Save yourown methods or choose from our broad rangeof pre-loaded measurement modes for simple,efficient analysis.


the flexibility and performanceyou expect from a LAMBDA

Measure DNA, RNA or Oliglo-nucleotides. Pathlength anddilution factors producedirect concentration results.

Data acquired at a definedwavelength will be multi-plied by a constant selectedby the user and the resultdisplayed.

Allows you to specify a singlewavelength to acquire data in%T or absorbance.

Calculates cell density toensure cell proliferation issufficient for induction orharvesting. Choose from ODunits or enter a factor tocalculate cells/ml.

Calculate protein concentra-tion by direct UV, BCA, Biuret,Lowry and Bradford methods.

Nucleic Acid Concentration and Purity Protein Determinations

ConcentrationSingle Wavelength

Bacterial Cell Culture Measurement


2008 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. LAMBDA is a trademark and PerkinElmer isa registered trademark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. Bluetooth is a registered trademark of Bluetooth SIG, Inc. Excel is aregistered trademark of Microsoft Corporation. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of theirrespective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.

008106_03


Speed, stability and confidence!Order online at www.perkinelmer.com/lambdabio

SPECIFICATIONS LAMBDA Bio LAMBDA Bio+

Optical design Split beam

Wavelength Range 190-1100 nmScanning range 200-950 nmCalibration Automatic when the instrument is switched onAccuracy 2 nmReproducibility 1 nm

Spectral bandwidth 5 nm 3 nm

Light source Pulsed Xenon lamp 5+ year lifetime

Photometric Range -0.300 to 2.500 A, 0 to 199 %TLinearity 0.005 A or 1% of the reading,

whichever is the greater at 546 nmReproducibility 0.003 A (0-0.5 A), 0.007 A (0.5-1.0 A)

Stray light

Its not just a UV/Vis.Its a LAMBDA.

One of the most trusted product lines in the industry is expanding.

When you see the name LAMBDA you know youre getting a quality instrument. Weve been manufacturingUV/Vis and UV/Vis/NIR instruments under the name LAMBDA for over 60 years. Wed like to introduceyou to the newest, smallest member of our family, the LAMBDA XLS a personal UV/Vis spectro-photometer for all your routine laboratory needs. Continuing on our tradition of providing great performance,the LAMBDA XLS is an instrument were confident in carrying the family name.

This is one time when big news comes in a small package.Intuitive, easy to use tools can save you both time and money. The LAMBDA XLS was designedspecifically with this in mind. With the latest innovative technology, this instrument allows you to investyour time in analyzing important data, rather than struggling to generate it. In addition, built-in, multiplelanguage configurations will allow you to select the language most applicable to you. Can you sayimpressive in several different languages? You will:

Impresionante Eindrucksvoll Impressionnant Formidabile (Spanish) (German) (French) (Italian) (Japanese)

Performance that wows the crowd and the finance people.With its large LCD screen and full scanning capabilities, the LAMBDA XLS is a crowd pleaser. Built tolast, easy to use and even easier to own. No moving parts, virtually no maintenance and no consumablesmake the LAMBDA XLS the perfect tool when the instrument just needs to work, day-in and day-out.Rely on the LAMBDA XLS. Its the smart choice for your laboratory in any application from teaching where operator training may be an issue, to the most advanced R&D laboratory where precioussamples need to be processed right, the first time. The LAMBDA XLS will win the hearts and mindsof anyone working with it. After all, it is a LAMBDA.

LAMBDA XLS and XLS+Spectrophotometers

Speed, stability and confidence!Order online at www.perkinelmer.com/lambdaxls

You dont need an impressive budget,to have an impressive instrument.The performance youll experience with theLAMBDA XLS is only normally associatedwith much more expensive designs. Withthe LAMBDA XLS youll experience:

Instant answers unlike other instrument on themarket, our LAMBDA XLS requires no warm up time.Our ultra fast initialization will have you analyzing a fullrange of spectrum in less than 3 seconds.

Continuous uptime our maintenance free designwith no moving parts, Xenon Light Source (XLS)and innovative polychromator technology providesyou the reliability your laboratory needs.

Superb stability avoid instrument drift from excessheat with our split beam design and external powersupply.

Ruggedness our sealed, zero bounce membranekeypad is resistant to even the greatest solvent spills.

Provides you with the versatility to dojust about anythingYou may work in a complex environment, but thatdoesnt mean your instrument needs to be. With the

simplicity and elegance of the LAMBDA XLS, youll beempowered to analyze samples when and whereyour projects demand:

See instant results capture a full range of spectrumin less than 3 seconds.

View, expand, and manipulate full spectra quicklyand easily with the large, clear 11.5 cm by 8.8 cmbacklit LCD display.

Feel confidence low stray light provides exceptionallinearity and confidence in your measurements.

Personalize your laboratory with the capacityto store up to 90 user developed methods.

Options to allow you to export data for processingor archiving.

Convenient sampling flexibility measure yoursamples in any of our high quality precision glass,quartz, or disposable cells:

10, 20 and 40 mm pathlength cells

Semi-micro cells: 700 l

Micro: 70 l

Ultra-micro cells: 15 l

2

designed to make

spectrophotometry easier

Our cell windows are fusedto the bodies using a specialprocess which avoidsdeformation of the windows.This assures that the fullwidth of the cell is useableand the finished celldimensions are maintained.

Share the knowledge multiplearchiving and data sharing options

Connect data from up to 8 instruments: asingle PC, using PVC application software can beconfigured to accept and automatically display datafrom up to 8 instruments. Data can be archived inExcel (.xls), ASCII (.csv or .txt), graphics (.emf), orrich text (.rtf) formats.

Built in USB port: transfer data to your computerfor viewing, manipulating, printing, or archiving usingthe built in USB connection port. LAMBDA XLS PVCsoftware provided as standard with all instruments.

Optional integrated thermal printer: Foryour permanent records, use the optional thermalprinter to print all your results. With the standard57 mm printer roll, your print-out can accommodateup to 40 characters per line plus graphics. Printedinformation includes:

Calibration Data

Result

Time and Date

Secure Digital (SD) Flash Storage Cardor Bluetooth compatible: for your archivingconvenience, the LAMBDA XLS can be outfittedto accommodate a standard SD flash storage cardor Bluetooth accessory allowing you additionalflexibility for transferring data.

Experience the power flexibleon-board software

The LAMBDA XLS software is optimized to provideyou with answers fast and conveniently. Save yourown methods or choose from our broad range ofpre-loaded measurement modes for simple efficientanalysis.


the flexibility and performanceyou expect from a LAMBDA

Allows you to specify a singlewavelength to acquire data in%T or absorbance

Calculates absorbance orconcentration ratios usingtwo defined wavelengths

Enables changes in absorbanceor concentration at user-definedwavelengths to be measuredas a function of time

Data acquired at a definedwavelength will be multi-plied by a constant selectedby the user and the resultdisplayed.

Data will be acquired atselected wavelengths anddisplayed in %T or absorbance

Up to 9 standards can be usedto develop and store a cali-bration curve which will beused to determine a sampleconcentration

You can define a spectral rangeover which spectrum will beacquired and displayed in%T or absorbance

Single Wavelength Spectrum Standard Curve

Absorbance RatioKineticsConcentrationMulti Wavelength


2008 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. LAMBDA is a trademark and PerkinElmer isa registered trademark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. Excel is a registered trademark of Microsoft Corporation. All othertrademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to changethis document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.

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Speed, stability and confidence!Order online at www.perkinelmer.com/lambdaxls

SPECIFICATIONS LAMBDA XLS LAMBDA XLS+

Optical design Split beam

Spectral bandwidth 5 nm 3 nm

Wavelength Range 190-1100 nmScanning range 200-950 nmCalibration Automatic when the instrument is switched onAccuracy 2 nmReproducibility 1 nm

Light source Pulsed Xenon lamp

Photometric Range -0.300 to 2.500 A, 0 to 199 %TLinearity 0.005 A or 1% of the reading,

whichever is the greater at 546 nmReproducibility 0.003 A (0-0.5 A), 0.007 A (0.5-1.0 A)

Stray light

Date post:	26-Nov-2015
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