Gas CH Chromatography l U.S. 800-323-4340 toll-free, 847-549-7600 (outside the U.S.), www.coleparmer.com Canada 800-363-5900 l India 91-22-6716-2222 l UK 0500-345-300 227 Table-of-Contents Web Registration Meet the demands of the busy analytical laboratory without taking up excess space—the Varian 3900 occupies less than half the workspace of many gas chromatograph (GC) units. Simple to use syringe trigger mechanism starts the GC upon injection. Electronic flow control of detector and injector eliminates knobs to turn off, thus saving you time, increasing long term precision of the GC, and saving you money on gases. Each GC features a front-access compact column oven with precise temperature control that uses standard 7" (17.5 cm) diameter capillary columns. Maximum operating temperature is 450°C—accommodating any application. Plus, the oven cools down from 450°C to 50°C in only 5.2 minutes! All models feature an icon-based keypad that simplifies training and increases daily productivity. Displays offer six pre- programmed languages and store up to five programs. Password protection locks keypad from unauthorized access. Automate the GC system to allow for reproducible results, high throughput, and unattended operation by adding an autoinjector or autosampler; order from facing page. Order Star Workstation software and 39001-50 ethernet communication kit for PC communication below. Optional Ethernet card 39001-52 is required for PC control if PC lacks 10 base-T Ethernet card; must be factory-installed and ordered at same time as GC. What’s included: Varian 3900 GC unit, split/splitless injector, column oven, and either flame ionization detector (FID) or thermal conductivity detector (TCD). Varian 3900 GC 39001-00 shown with autoinjector 39001-30 from page 228 Specifications & Ordering Information FID Detector Detectivity: 2 picograms of carbon per second Flame-out detection: yes Auto re-ignition: yes Data acquisition rate: up to 80 Hz Split/Splitless Injector Pressure range: 0 to 100 psi Total flow: 500 mL/min at 10 psi Communication Analog output: 0 to 1 V or 0 to 10 V (standard) Digital output and control: TCP/IP with industry- standard Ethernet 10 Base-T (optional) System Specifications Operating temperature range: 10 to 35°C (50 to 95°F) Power requirements Models 39001-00 and -10: 120 VAC ± 10%, 60 Hz ±2%, 6 amps, 1.3 kW Models 39001-05 and -15: 230 VAC ± 15%, 50 Hz ±2%, 6 amps, 1.3 kW Dimensions: 12 1 ⁄2"W x 21 4 ⁄5"H x 22"D Column Oven Temperature range: ambient + 10°C to 450°C Cool-down rate: 450°C to 50°C in 5.2 minutes Temperature resolution: 1° TCD Detector Detectivity: 300 pg/mL (butane) Linear dynamic range: 106 (butane) Filament protection: yes, standard Automatic bridge balancing: yes, standard Gas Chromatography Second Edition. By I. Fowlis, 1998. Presents the basic principles and theory behind the technology and incorporates the most important recent advances in the field. EK-15263-27 Gas Chromatography, 2nd Edition 258 pp. ISBN 0-471-95468-3 Varian ® 3900 Gas Chromatograph Systems Automated GC system in space-saving footprint l Simplified routine operation icon-based keypad maximizes efficiency Catalog number Detector Power (VAC, Hz) Shpg wt lb (kg) Price KH-39001-00 FID 120, 60 80 (36.3) KH-39001-05 230, 50 KH-39001-10 TCD 120, 60 80 (36.3) KH-39001-15 230, 50 ColeParmer.com/Chromatography Search online for all our new items— product lines are continually updated! Star Workstation software lets you control, collect data, and set user access for the Varian 3900 GC system. KH-39001-56 Star workstation software for single Varian GC unit KH-39001-58 Star workstation software for up to four Varian GC units KH-39001-60 Star custom report writer lets you create custom and automated reports in a variety of formats KH-39001-50 Ethernet communication kit. Required by all GC models for PC control KH-39001-52 Ethernet communication card. Required for PC control if PC lacks 10 base-T Ethernet card (PCI bus). Factory-installed; must be ordered at same time as GC KH-39001-54 Analog cable with sync, spade lugs. Required by models 39001-00 and -05 for PC control Software and Communication Accessories www.coleparmer.com
Transcript
GasCHChromatography
l U.S. 800-323-4340 toll-free, 847-549-7600 (outside the U.S.), www.coleparmer.com Canada 800-363-5900 l India 91-22-6716-2222 l UK 0500-345-300
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Meet the demands of the busy analytical laboratory without taking up excess space—the Varian 3900 occupies less than half the workspace of many gas chromatograph (GC) units. Simple to use syringe trigger mechanism starts the GC upon injection. Electronic flow control of detector and injector eliminates knobs to turn off, thus saving you time, increasing long term precision of the GC, and saving you money on gases.
Each GC features a front-access compact column oven with precise temperature control that uses standard 7" (17.5 cm) diameter capillary columns. Maximum operating temperature is 450°C—accommodating any application. Plus, the oven cools down from 450°C to 50°C in only 5.2 minutes!
All models feature an icon-based keypad that simplifies training and increases daily productivity. Displays offer six pre-programmed languages and store up to five programs. Password protection locks keypad from unauthorized access.
Automate the GC system to allow for reproducible results, high throughput, and unattended operation by adding an autoinjector or autosampler; order from facing page. Order Star Workstation software and 39001-50 ethernet communication kit for PC communication below. Optional Ethernet card 39001-52 is required for PC control if PC lacks 10 base-T Ethernet card; must be factory-installed and ordered at same time as GC.
What’s included: Varian 3900 GC unit, split/splitless injector, column oven, and either flame ionization detector (FID) or thermal conductivity detector (TCD).
Varian 3900 GC 39001-00 shown with autoinjector 39001-30
Dimensions: 121⁄2"W x 214⁄5"H x 22"DColumn OvenTemperature range: ambient + 10°C to 450°CCool-down rate: 450°C to 50°C in 5.2 minutesTemperature resolution: 1°TCD DetectorDetectivity: 300 pg/mL (butane)Linear dynamic range: 106 (butane)Filament protection: yes, standardAutomatic bridge balancing: yes, standard
Gas ChromatographySecond Edition. By I. Fowlis, 1998. Presents the basic principles and theory behind the technology and incorporates the most important recent advances in the field.
EK-15263-27 Gas Chromatography, 2nd Edition 258 pp. ISBN 0-471-95468-3
Varian® 3900 Gas Chromatograph SystemsAutomated GC system in space-saving footprintl Simplified routine operation icon-based keypad
ColeParmer.com/ChromatographySearch online for all our new items—product lines are continually updated!
Star Workstation software lets you control, collect data, and set user access for the Varian 3900 GC system.
KH-39001-56 Star workstation software for single Varian GC unit KH-39001-58 Star workstation software for up to four Varian GC units KH-39001-60 Star custom report writer lets you create custom and automated reports in a variety of formats KH-39001-50 Ethernet communication kit. Required by all GC models for PC control KH-39001-52 Ethernet communication card. Required for PC control if PC lacks 10 base-T Ethernet card (PCI bus). Factory-installed; must be ordered at same time as GC KH-39001-54 Analog cable with sync, spade lugs. Required by models 39001-00 and -05 for PC control
Software and Communication Accessories
www.coleparmer.com
GasCH Chromatography
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Versi-Vials provide superb performance at an economical priceMany types to choose from—snap, wide neck, screw-thread or shell vials with plugs.l Limited-volume inserts feature
bottom springs that eliminate costly needle damage
l Graduated marking spots enhance volume identification
See page 1965 for details
Flat-bottom insert
98730-04 shown
inside vial 98721-01
Bottom-spring insert
98730-00 shown
inside vial 98722-01
Varian® 3900 Gas Chromatograph System (continued)
Automation Accessories
Autosampler features a 100 x 2 mL vial capacity carousel and is ideal for labs which require high throughput and conformational analyses. The possibility of bent needles is greatly reduced as the autosampler aligns the syringe over the injector nut before each injection.
KH-39001-20 Varian 8400 GC autosampler
Both the autoinjector and the autosampler offer two injection modes which provide an exceptional level of sample throughput. Dual injection mode injects two different samples into two different injectors at the same time. Duplicate injection mode injects the same sample into two different injectors sequentially; an improvement over splitting the sample between two columns with a single injection. Neither of these modes requires manual intervention. Standards or matrix spikes can be automatically added.
A waste drain and three separate wash vials eliminate sample carryover. Program any, all, or a combination of pre- and post-solvent flushes during method set-up. Both units prevent volatile solvent evaporation; sample trays and syringe needles are positioned away from heated surfaces.
l For increased speed and efficiency in analyzing GC samples
Autoinjector offers labs increased flexibility as the operator can select three different sample sizes: 2 mL, 5 mL, and 10 mL. All three size vials are held on a fixed carousel. To help prevent evaporation of volatile solvents, the autoinjector does not position samples directly over the injector port.
KH-39001-30 Varian 8410 GC autoinjector
Autoinjector 39001-30
Autosampler 39001-20
GasCHChromatography
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Temperature range: 25 to 400°CTemperature control: programmable, with unlimited
ramp and dwell timesTemperature stability: ±0.1°C
Heating rate: 50°C per minute to 300°C; 20°C per minute from 300 to 400°C
Cooling rate: 300 to 50°C in 5 minutesGas flow control: electronic pressure controlFittings: 1⁄8" compression
Dimensions Oven: 8"W x 3"H x 8"D Overall: 181⁄2"W x 10"H x 141⁄2"D
Power: 115 or 220 VAC, 50/60 Hz; 115 VAC model includes 6-ft cord with grounded plug
Specifications & Ordering Information
Optional purge and trap
Compact Gas ChromatographsLow-cost, high-quality analysisl Use capillary or packed columns for optimal separationsl Analyze data with sophisticated software These economical, durable gas chromatographs provide all the functionality you need. Achieve excellent separations under digitally controlled conditions. Versatile detector options provide response over a broad range of compounds. Sophisticated data analysis software enables you to quickly quantify, compare, and validate results.
System ConfigurationAll gas chromatographs will accept 0.53-mm capillary columns or 1⁄8" packed columns. On-column injector is standard. See below for detector descriptions and system selection.
System ControlControl temperature (ambient to 400°C) and pressure simply and precisely using a 20-bit A/D board. Interface using PeakSimple software for temperature, pressure, and gradient control. Unit LCD shows temperature and set point. Program unlimited ramp and dwell times plus carrier gas pressure. Utilize eight TTL outputs to achieve device contact closures.
SoftwareAll gas chromatographs come with PeakSimple analysis package for Windows®. PeakSimple enables everything from integration and data smoothing to chromatogram overlays, 3D multiple displays, validation, and other advanced features. PeakSimple is also the system interface for gas chromatograph setup, control, data acquisition, and dynamic data exchange (DDE) linkages with other software.
Oven-cooling fans Tilt-up cover with interlock switch
Injection portColumn
oven Digital temperature display and selector
switch
Detector
34009-40
Detector / System SelectionA Gas Chromatograph with Thermal Conductivity Detector (TCD) is used for gas analysis or for simple mixtures. TCD responds well to oxygen, nitrogen, water, and other nonhydrocarbons with detection limits in the 100 ppm range. Filament protection eliminates TCD burnout.
B Gas Chromatograph with Flame Ionization Detector (FID) detects hydrocarbon down to 1.0 nanogram and responds linearly over a majority of its range. Hydrocarbons ionize in the hydrogen gas flame and are attracted to a metal collector electrode. Ceramic ignitor glows permanently to prevent flameouts and re-ignites when flame is flooded with water.
C Combination TCD and FID Detector is connected in series, allowing you to perform two analyses of the same sample at once.
PeakSimple analysis software (included)
For chromatography reference books, look under ”Analytical Chemistry” in our online “Cole-Parmer Bookstore”.
Key letter Catalog number Description Power Price
A KH-34009-10 Gas chromatograph with 115 VAC, 50/60 Hz KH-34009-15 thermal conductivity detector (TCD) 220 VAC, 50/60 Hz
B KH-34009-20 Gas chromatograph with 115 VAC, 50/60 Hz KH-34009-25 flame ionization detector (FID) 220 VAC, 50/60 Hz
C KH-34009-40 Gas chromatograph with combination thermal 115 VAC, 50/60 Hz KH-34009-45 conductivity and flame ionization detector (TCD/FID) 220 VAC, 50/60 Hz
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Chrysalis® II Hydrogen GeneratorsReliable, continuous output of >99.9999% pure hydrogen using only DI waterl Safe alternative to dangerous and expensive laboratory gas cylinders l LCD display and membrane keypad for easy operationl High performance models are maintenance-free—no messy desiccant cartridges to replaceChyrysalis II hydrogen generators feature the newest membrane technology available for electrolytic production of pure hydrogen to ensure reliable, controlled gas delivery. Digital display provides system operating status and diagnostic capabilities. Multiple alarms alert user to low water level, poor water quality, and low pressure conditions to maintain optimum generator performance. Unit is powerful, yet compact—small footprint conserves laboratory benchtop space. Ideal for gas chromatography applications or as a fuel gas for flame ionization detectors.
Chrysalis II hydrogen generators are available in multiple styles and flow rates. Standard model uses desiccant cartridge for final purification and moisture removal. High performance model features an innovative internal drying system to remove moisture for maintenance-free operation without desiccant cartridges.
Call our Application Specialists for more information on gas chromatography.
847-549-7600
86441-10
Chrysalis® II ZERO Air GeneratorsEliminate the need for high-purity air cylindersl Compact, energy efficient, and easy to installl Minimal maintenance requiredZERO air generators continuously produce high-purity air from plant compressed air—and remove particulates down to 0.5 microns, hydrocarbon and CO impurities to less than 0.1 ppm, and moisture to less than 70°C dew point. These generators conserve valuable laboratory space by eliminating the need for high-purity air cylinders. Eliminate the need to recalibrate instruments after replacing air cylinders and the costs of replacement cylinders.
Models feature indicating lights for power, warm-up, ready and fault notification of low/high catalyst temperature, low/high inlet pressure, and life expectancy/expiration of catalyst. Digital thermal switch automatically shuts off power to the catalyst in the event the air supply is shut off.
What’s included: power cord with plug.
KH-86441-70 Replacement external air filter (housing and element) KH-86441-74 Replacement cartridge for external air filter KH-86441-78 Replacement cartridge for internal air filter
Specifications & Ordering Information
Catalog number Capacity Inlet air pressure Power PriceKH-86441-30 1.5
Outlet temperature: ambient 59°F (1°C)Connections: ¼" inlet, 1/8" outlet Dimensions: 16"W x 18"H x 10"D
86441-30
GasCHChromatography
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Column Packing AccessoriesKH-01999-70 Glass wool; deactivated, silane treated, 50 g KH-01999-72 Glass wool; deactivated, HMDS treated, 10 g KH-01999-60 Column vibrator for tight, consistent packing, 115 VAC KH-34670-00 Tubing cutter features a sharp adjustable wheel blade that cuts tubing 1⁄8" to 11⁄8" thick. Use on copper, brass, and aluminum tubing only KH-34670-10 Deburring tool lets you make tight connections. Sharp blades remove burrs inside and outside all 1⁄8" to 15⁄8" OD tubing
Tubing and Accessories
304 SS tubing 34680-00
Electric SS Tubing CutterClean, burr-free cuts improve chromatographic reproducibilityl Assures zero dead volume tubing
connections in chromatography applicationsl Eliminates sample dispersion caused by
improper deburring of tubingEliminate burrs and uneven cuts on stainless steel and copper tubing for chromatography applications. Cutting wheel produces a square cut on tubing. Use with any HPLC or chromatography pilot scale plumbing. Dressing tool for 1⁄4" and 1⁄8" OD tubing available separately.
What’s included: dressing tool for 1⁄16" OD tubing.
95649-00
EK-95649-50 Dressing tool for 1⁄8" OD tubing EK-95649-55 Needle insert for 1⁄16" OD dressing tool EK-95649-60 Replacement cutting wheel. Pack of 3
Packed ColumnsPacked stainless steel columns are great for the analysis of gas and low molecular weight species. Columns measure 3-ft long x 1⁄8" OD. Model 34005-05 is a double column; outer column is 1⁄4" OD, inner column is 1⁄8" OD.
Catalog number Description Application Price
KH-34005-00 Silica gel Use for C1-C6 gas separations; good for separating air and CO2
KH-34005-01 Molecular sieve
Separates oxygen, nitrogen, methane, and CO; not good for heavier molecules
KH-34005-02 Porapak-Q Use to separate water from solvents and C1–C8 hydrocarbons
KH-34005-05 CTR-1 Double column designed to separate oxygen, nitrogen, methane, CO, and CO2
Catalog number Description PriceEK-95649-00 Electric SS tubing cutter, 110-120/220-240 V
Catalog number Description Price/50-ft rlStainless steel tubing
KH-34680-00 SS tubing, 1⁄8" OD KH-34680-10 SS tubing, 1⁄4" OD
Copper tubingKH-34671-00 Copper tubing, 1⁄8" OD x 0.065" ID KH-34671-10 Copper tubing, 1⁄4" OD x 0.190" ID
304 Stainless Steel TubingMake your own GC columns; simply cut tubing to desired length and coil to fit in your gas chromatograph. Special cleaning process eliminates residual oils and dirt; tubing is endcapped to keep it clean. Max pressure is 7200 psi. Fittings are not included. Roll of 50 ft.
Copper TubingCut this copper tubing to desired length to plumb gas lines into your gas chromatograph. Maximum pressure: 2800 psi. Roll of 50 ft.
Oxygen TrapsReduce residual oxygen content to less than 0.1 ppm with standard gas chromatography flow rates. Indicating material changes color from bright green to gray when depleted. Plastic sleeve protects glass housing in case of breakage. Traps measure 101⁄4"L x 13⁄8" dia.
Moisture TrapsGlass trap removes moisture while eliminating potential contamination from plastic. Graphite seal ensures purity and sealing at pressures up to 100 psi. Plastic outer tube provides protection in case of breakage. Adsorbent materials (100-g) are indicating Drierite® (Calcium sulfate with cobalt chloride) and 5-Å molecular sieve; a 40-µm stainless steel screen removes fine particulates. Drierite® changes color from blue to pink when depleted—easily regenerated. Trap is 101⁄4"L x 13⁄8" dia.
Carbon Dioxide TrapsTraps remove carbon dioxide from your gas stream. A 500-mL nickel-plated carbon steel cylinder holds packed absorbent (80% emulsive, 320 g; 20% NaOH, 70 g); withstands up to 1000 psi. Include drying stage to prevent moisture from entering system. If downstream moisture is harmful to application,a second drying stage is recommended.
Trap measures 15"L x 2" dia; frits are integral to each fitting. Regeneration or refilling is not recommended.
Traps Remove impurities to prolong column life and reduce detector interference
Large-Capacity Moisture TrapsTraps remove moisture, oils, and dust from carrier gases. Large 400-mL capacity means fewer refillings are required. Adsorbent materials are indicating Drierite® and 5-Å molecular sieve. Drierite® changes color from blue to pink when depleted. Easily refill the trap with the refill kit sold below. The silicone O-ring seals maintain pressures to 100 psi. Traps feature transparent acrylic housing. Measure 17"L x 2" dia. Choose a trap with base plate for benchtop mounting.
KH-34651-39 Moisture trap refill kit for large-capacity traps only
01997-20
34651-60
34651-00
01997-00
Catalog number Volume Fittings Base plate PriceKH-34651-00 400 mL
1⁄8" No KH-34651-10 1⁄4" KH-34651-20 400 mL
1⁄8" Yes KH-34651-30 1⁄4"
Catalog number Volume Fittings PriceKH-01997-20 55 mL 1⁄8" KH-01997-22 55 mL 1⁄4"
Catalog number Volume Fittings PriceKH-34651-60 40 L
1⁄8" KH-34651-70 1⁄4"
Catalog number Volume Fittings PriceKH-01997-00 40 mL
1⁄8" KH-01997-02 1⁄4"
GasCH Chromatography
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SeptaMarathon™ Septa have outstanding durability in either autosampler or manual injection applications. Operate at a maximum temperature of 400°C and achieve up to 400 injections per septum. Pre-piercing eliminates injection coring on select CenterGuide™ septa.
BTO® (Bleed and Temperature Optimized) Septa are designed for low bleed and temperatures up to 375°C. Septa changes are easy—septa will not stick to your port. Pre-piercing eliminates injection coring on select CenterGuide septa.
Advanced Green 3 Septa are low-bleed septa that are specifically created to combine a long injection life with low injection port adhesion to meet even your most challenging analysis. Maximum temperature 305°C. Pre-piercing eliminates injection coring on select CenterGuide septa.
Puresep-P™ Septa are made of silicone rubber with a stable layer of polyimide film. Maximum temperature 572°F (300°C).
Puresep-T™ Septa are made of Teflon® and polyimide films on silicone rubber bases. Teflon gives maximum inertness and low bleed. Maximum temperature: 437°F (225°C).
FerrulesVespel®/Graphite Ferrules are one-piece design requiring no back ferrule. Maximum temperature: 400°C. Recommended for GC/MS interface applications. Reusable—does not seize into column.
Graphite Ferrules are preferred for capillary columns. Maximum temperature: 450°C. Does not stick to glass and is reusable if not over- tightened. One-piece design—no back ferrule.
Graphite ferrules
Vespel/graphite ferrules
Marathon septa 01999-00
BTO Septa 01999-10
Advanced green septa 01999-20
Puresep-T septa 01994-20
Puresep-P septa 01994-02
Catalog number Description Price/pk of 50Marathon™ septa
Description For tube size (ID)Vespel/graphite Graphite
Catalog number Price/pk of 10 Catalog number Price/pk of 10Capillary column ferrules
1⁄16" to 0.4 mm 0.25 mm KH-01998-60 KH-01998-50 1⁄16" to 0.5 mm 0.32 mm KH-01998-62 KH-01998-52 1⁄16" to 0.8 mm 0.53 mm KH-01998-64 KH-01998-54
Short ferrules for Agilent inlets1⁄16" to 0.4 mm 0.25 mm KH-01998-66 KH-01998-56 1⁄16" to 0.5 mm 0.32 mm KH-01998-68 KH-01998-58 1⁄16" to 0.8 mm 0.53 mm KH-01998-69 KH-01998-59
Gas PurifiersRemove contaminants from source gases for better results!Purify research-grade helium for gas chromatography applications. Choose either an all-in-one system for removing most common contaminants, or an individual cartridge purifier for single contaminant removal. Mount on lab walls or benches—hardware included.
A All-in-One Gas Purification SystemsOne cartridge efficiently removes oxygen, water, sulfur compounds, and hydrocarbons. Removes oxygen and water to < 0.2 ppm. Choose either 1⁄8" or 1⁄4"NPT fittings.
B Individual Cartridge PurifiersRemoves a specific contaminant from your system. Mix and match purifier cartridges to meet your specific application. Choose either 1⁄8" or 1⁄4" connection fittings. Order replacement cartridges below.
Individual purifiers are ideal when you need to remove only
one contaminant.
All-in-one gas purification
systems 01997-10
BA
DescriptionPurifiers with 1⁄8" connection fittings Purifiers with 1⁄4" connection fittings Replacement cartridges
Catalog number Description Fitting size PriceKH-01997-10
All-in-one system1⁄8" NPT
KH-01997-12 1⁄4"NPT
GasCHChromatography
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Flame Detector Cleaning KitKit includes a set of jet reamers, two wire minibrushes, and a dual-ended handle for reamers and brushes.
Fine Wire Cleaning KitUse this kit to clean syringes, needles, and more. Includes ten 0.15-mm SS wires, ten 0.25-mm SS wires, ten 0.50-mm SS wires, and a pin vise to hold wire during use.
Gas Chromatography Cleaning and Maintenance Kits Prevent erroneous peaks from appearing in chromatograms
Syringe Accessories
Capillary Maintenance KitIncludes lighted magnifier, tweezers, ceramic-tipped fused silica cutter, three column scribes, two universal unions, single-ended pin vise, round needle file, multipurpose microhook, 15-cm ruler, three drill bits (0.4 mm, 0.5 mm, and 0.8 mm), one 1⁄4" to 5⁄16" open-ended wrench, three nylon brushes (1⁄8", 3⁄16", and 1⁄4"), two 1-foot lengths of pipe cleaner (1⁄8" and 1⁄4"), one 3-ft length of high-temperature string, and ten chromatogram labels.
01996-50
Chaney Adapter ensures reproducibility of better than ±1%; use when injections of identical volumes are required.
Repeating Dispenser delivers 1⁄50 of total syringe volume with each actuation. Order the dispenser that fits your syringe size.
Syringe Guides prevent the plunger from bending or pulling out of your small-bore syringe.
34650-00
Gastight® High-Performance SyringesRemain gas- and liquid-tight up to 200 psi!These essentially inert syringes have Teflon®-coated plungers (50-µL and 100-µL models have stainless steel plunger with removable Teflon tip). Barrels and plungers are interchangeable within each syringe size, allowing field repair. Note: Needle is not included with Luer tip and Teflon Luer-lock syringes; order CTFE-hubbed hypodermic needles below.
Syringe 07939-02 with cemented
needle
Syringe CleanerEliminate cross-contamination and peak ghostingVaporize hard-to-clean needle residues quickly and easily. Syringe cleaner helps ensure the integrity of your analysis with a 250°C cleaning that requires only 30 seconds and a few pumps on the syringe plunger. Thermostatically controlled unit accepts needles up to 1 mm in diameter and 100 mm long. A vacuum source hookup is recommended for proper operation.
AutosamplersUses minimum bench space—fits even in hooded enclosuresl Removable, 20-vial carousel uses standard autosampler vialsl Remote control capabilities—start injection and initiate data acquisition by contact closure
Our low-cost autosamplers for your HPLC work are fully programmable from front-panel controls. Program sample size/injection volume (recommended three to five times loop volume) and the number of injections. One or more injections may be programmed from the same vial. Ensure accuracy by using fixed loop or partial loop injection. Perform manual injection via easily accessible injection port. Electrically activated unit features automatic wash rinse between samples.
What’s included: a 20-vial carousel, one hundred 11-mm vials, external wash reservoir, and chromatography support software.
42664-50
HPLC SystemsInstruments for your performance requirements and budgetary constraints
Our HPLC systems provide the functionality necessary to achieve precise high-quality separations for analytical and preparative methods. Select from robust 3000 PSI models (PEEK construction) or 5000 PSI models (stainless steel construction) with all the features needed for consistent results and total component integration for ease of use. Pumps utilize a built-in pulse damper for smooth, low-pulse flows. Features include solvent delivery, gradient/isocratic control, 20 µL injection loop, UV-Vis detection, and data acquisition and processing.
Isocratic HPLC Systems with Variable Wavelength DetectorsUse these precision instruments for routine separation and analysis of compounds absorbing in the 195 to 800 nm range. For analytical and preparative applications not requiring gradient capability. All wettable parts are constructed of chemical-resistant materials (PEEK, PTFE, SS). Select wavelength with scrolling controls, autozero, and mark events from the front panel. Digital display of absorbance and wavelength. Analog outputs for data acquisition or chart recorder. Use graphics, integration, and analytical tools in Star-Chrom Lite™ software (sold separately at right).
Automated Binary Gradient HPLC Systems with Variable Wavelength DetectorsThese versatile systems provide integrated HPLC capabilities that meet the needs of most lab applications. Beyond the features of the isocratic systems sold above, these systems include a second solvent pump, a data acquisition system for feedback/control, and Star-Chrom computer software for Windows® NT/XP/2000. Solvent mixing takes place in a precision mixing tee monitored by pressure transducer. Use the software for gradient and other parametric setups, data acquisition and system control, complete graphical/analytical capability, method storage, control of automated sampling and fraction collection. Connection to computer is RS-232; computer not included.
42661-04
Catalog number Pressure Voltage Dimensions
(W x H x D) Price
Isocratic HPLC Systems with variable wavelength detectorsKH-42661-04 3000 psi 115 77⁄8" x 71⁄8" x 16" KH-42661-06 230 KH-42663-30 5000 psi 115 77⁄8" x 71⁄8" x 16" KH-42663-35 230
Automated binary gradient HPLC systems with variable wavelength detectorsKH-42664-10 3000 psi 115 77⁄8" x 71⁄8" x 16" KH-42664-15 230 KH-42664-20 5000 psi 115 77⁄8" x 71⁄8" x 16" KH-42664-25 230
Flow rate: 0.01 to 9.99 mL/minDisplay: absorbance, wavelength,
Drift: <0.0005 AU/hrNoise: <0.00005 AUFlow cell, analytical: 7.0 mm path,
10 µL volumeRecorder output: 10.0 mVAnalog output: 1.0 to 2.0 V (2.0 A)
Specifications & Ordering Information
KH-42661-80 Star-Chrom Lite software, 115 VAC KH-42661-85 Star-Chrom Lite software, 230 VAC
Star-Chrom ™ computer software
42664-10 (PC not included)
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See page 235 for chromatography refrigerators.
CHChromatography
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Binary Gradient SystemsFunctionality and flexibility in a simple-to-use systeml EZStart™ software reduces control to just three steps: configure, run, and reportl Flow rates between 0.001 to 5 mL/minl Time-programmable UV/Vis detectorRugged systems bring dependability and reliability to the lab. EZStart™ software offers comprehensive control features in an easy-to-use system. Model 42662-50 uses a variable-wavelength UV/Vis detector ideal for routine applications. Model 42662-52 provides single- and dual-wavelength capabilities with spectral scanning, multiple method storage, file linking, and more. Advanced pump features include rapid-refill cam and prime-purge valve/low-volume pulse dampener, giving minimal baseline noise and low pulsation.
What’s included: binary pump, UV detector, EZStart™ software, mixing kit, injection valve with 20-mL loop and tubing.
Catalognumber Detector type Max
pressure Power Price
KH-42662-50 Single variable wavelength 6000 psi 110 to 220 VAC, 50/60 Hz
KH-42662-52 Dual wavelength
42662-50
Variable-Volume Autosampler, with 120-vial capacity, offers multiple injection modes, random vial access, reusable calibration standards, and nonvolatile storage of up to four complete methods.
KH-42662-60 Variable-volume autosampler; 110 to 220 VAC, 50/60 Hz
Fluorescence Detector provides high sensitivity and exceptional flexibility for routine and trace analyses. Time programming of excitation and emission wavelengths let you optimize sensitivity and selection. Store up to four user files with ten timelines each for easy wavelength changes.
KH-42662-65 Fluorescence detector; 110 to 220 VAC, 50/60 Hz
PC Preconfigured with EZStart Software provides a ready-to-use control system making start-up easier. Includes a flat-panel monitor.
KH-42662-70 PC with EZStart software and flat-panel monitor
Accessories
Chromatography RefrigeratorsIdeal for use with entire thin layer chromatography (TLC) tank assembliesDesigned for the chromatography lab, these refrigerators feature two, 2"-diameter (5.1 cm) capped access portholes in each side wall to permit the insertion of thermocouples and other wiring without disturbing temperature settings. Push-button lights and circuit breaker-protected duplex electrical receptacles simplify equipment operation and allow observation of ongoing separations.
Units feature microprocessor-controlled thermostats and digital temperature display for precise temperature control, and come with 21⁄2"-thick foamed-in-place, CFC-free urethane insulation. Rust-resistant, phosphate-impregnated steel cabinets are coated with baked-on enamel for a durable finish.
What’s included: two sliding thermopane glass doors, epoxy-coated, steel-wire shelves, keyed ON/OFF power switch, keyed door lock, and adjustable stainless steel legs.
Interior light Model 44260-60: 30-watt fluorescent Model 44260-70: 40-watt fluorescent
Lock-out control: all doors are keylocked
Adjustable legs: four levelersPower cord: NEMA 5-20P
(requires NEMA 5-20R receptacle)
44260-70
Specifications & Ordering Information
Specifications & Ordering Information Flow rate: 0.001 to 5 mL/minPump accuracy: ±2%Pump precision: 0.2% RSD
Drift: <0.0002 AU/hrNoise: <0.000025 AU
Analog outputs: range selectable over entire absorbance range
Catalognumber Capacity Temperature
rangeDimensions (W x H x D) No. of
shelvesAmps/
breakerPower
(VAC, Hz)Shpg wtlb (kg) Price
Interior Overall
EK-44260-60 38 cu ft (1076 L) Adjustable from1 to 8°C (34 to 46°F)
401⁄2" x 55" x 251⁄2" 44" x 78" x 311⁄2" 2 half-depth,
16/20 115, 60456 (207) (103 x 140 x 65 cm) (112 x 198 x 80 cm) adjustable;
EK-44260-70 45.4 cu ft (1285 L) 49" x 55" x 261⁄2" 52" x 78" x 311⁄2" 6 full-depth, 533 (242) (124 x 140 x 67 cm) (132 x 198 x 80 cm) adjustable
CH Chromatography
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Liquid Chromatography DetectorsSensitive, affordable detectors for HPLC and low-pressure chromatographyReplace your failed component or augment your liquid chromatography system with these precision UV/Vis detectors. All wetted parts are PEEK, PTFE, or Tefzel® for excellent chemical resistance and biocompatibility. Integrate detector into your data stream via analog output.
Fixed Wavelength DetectorsUse these durable detectors for your repetitive analysis and preparative techniques. Standard 254 nm detection wavelength can be changed using optional filter conversion kits. Adapt for preparative operation using shorter path length flow cells (sold separately below right).
Variable Wavelength DetectorsThese versatile instruments have double beam optics, chemical-resistant fluid contact surfaces, and a small footprint. Select your UV/Vis wavelength (195 to 800 nm) using front-panel scrolling or remote computer-driven contact closures. Digital readout and analog outputs for wavelength and-absorbance.
High-Precision Piston PumpsDeliver precise volumes at pressures up to 6000 psi!l Ideal for use in HPLC applicationsl Pulse dampener significantly reduces
pulsation with low dead volume. 74930-10
Economical Digital PumpDigital pump provides precise, reproducible flow in an economical package. Single-piston pump significantly reduces pulsation through the use of an advanced electronic rapid-refill design.
Microprocessor-controlled pump provides control signals to the motor drive circuits, interfaces with the keypad and digital LED display, receives signals to refill based on flow rate, and provides RS-232 communication. Other features include run/stop inputs, dual-voltage capabilities, self-flushing pump heads, and a motor stall detector.
What’s included: a 6-ft power cord with plug.
Constant-Flow HPLC PumpUse this dual head pump for analytical to small-scale chromatography applications. Constant-flow pump can be used with columns from 2 to 22 mm ID.
Drive features a linear cam and pressure compensation to provide accurate gradients in high- or low-pressure modes. Chemical-resistant keypad and digital LED display allow flow adjustments in 0.01-mL increments. Other features include a RS-232 serial communication port, run/stop inputs, remote frequency andvoltage controls, dual voltage capability, and real-time pressure monitoring.
Maxpsi Pump head Repeatability Wetted parts Dimensions
(W x H x D) Price
A EK-74930-00 0.01 to 10 3000 PEEK ±0.5%PEEK check valve body and outlet tubing; synthetic ruby and sapphire check valve internals; sapphire piston; PTFE inlet tubing and pulse dampener diaphragm.
73⁄4" x 61⁄4" x 123⁄4"
A EK-74930-05 0.01 to 10 3000 316 Stainless steel
±0.5% 316 SS check valve body and tubing; synthetic ruby and sapphire check valve internals and piston; fluorocarbon pulse dampener diaphragm.
73⁄4" x 61⁄4" x 123⁄4"
B EK-74930-10 0.01 to 12 6000 ±0.2% 11" x 6" x 18"
Specifications & Ordering InformationMaximum temperature (fluid):
212°F (100°C)Operating ambient: 32 to 176°F
(0 to 80°C) with max 98% humidityDuty cycle: continuousSelf-priming: no
Accuracy: ±2%Power: 110 to 220 VAC, 50/60 Hz
*Description and complete information can be found on page 1389.
1389
See page 1389 for additional high-precision, high-pressure piston pumps.
CHChromatography
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AccessoriesEK-29707-50 Single-position handheld compressor EK-29707-52 Six-position compressor *Contact our Application Specialists for a list of approved autosamplers.
Specifications & Ordering Information
Catalog number Material Pore size Qty/cs Price/csEK-29707-00 Nylon 0.45 µm 100 EK-29707-02 PTFE EK-29707-04 PVDF 0.45 µm 100 EK-29707-06 PP
Generate less lab waste and increase cost savings per test by replacing your existing syringe-coupled filtration devices and autosampler vials with this single disposable unit. Filter incorporates filtration membrane on one end of plunger and preattached cap/septum on the other. Depress plunger to force liquid through membrane into plunger reservoir. Mini-UniPrep filter is then ready to be placed into any approved* autosampler designed to accept 12-mm H x 32 mm dia vials. For more filter options including amber housing, see page 467.
What’s included: handheld compressor 29707-50. Use compressor for individual Mini-UniPrep filters or order processor 29707-52 for six-position parallel filtration. Processor is used in conjunction with Mini-UniPrep stand, which holds up to 60 samples.
Use the six-position compressor 29707-52 for easy parallel filtration.
29707-00
Combine modes for more versatilityCreate multi-function modes by combining any of the four following nozzle movement modes.
Simple Mode controls dispensing or collection of a uniform quantity in each test tube for the entire sequence of tubes.
Window Mode lets you set up to 10 sample collection time windows and vary the interval between sample collections.
Peak Mode allows you to collect up to 10 individual absorbance peaks in separate tubes. At start, sample is discharged to wait drain if level is below the programmed peak value. Sample collection in the tubes begins when the detected absorbance level exceeds the programmed level. Between peaks, the sample is collected in a separate line of tubes. Use three-way valve 01473-93 for operation in peak mode; order separately below.
Bottle Mode lets you collect individually selectable quantities in larger-volume bottles.
Automate your fractioning procedures with our programmable fraction collector. Features three fractionation modes (time, drop count, and external count), and four-nozzle movement modes. A shifting-type nozzle that lets you deliver liquids into specified tube position. Built-in wait drain, with programmable interval, allows discharge of buffer or waste solution apart from the collection tubes.
Enter programs by following easy-to-use prompts on the LED display. Retrieve stored procedures, from battery back-up memory, for automatic repetition. Collector also was a built-in heater for cold room use and a self-check function.
What’s included: a test tube rack that holds 120 tubes in a 12 x 10 array (accommodates 90- to 180-mm H x 12- to 18-mm dia tubes), test tube tray for placement under rack (121⁄2"L x 81⁄2"W x 21⁄2"H; 318 mm x 216 mm x 64 mm), 61⁄2-ft length of 1-mm ID PTFE tubing, and a 6-ft power cord with U.S. standard plug.
KH-01473-93 Optional three-way valve
Catalog number Description PriceKH-01473-90 Programmable fraction collector
Nozzle movement: pulse-controlled stepper motor
Nozzle movement rate: 0.15 sec minimum (simple mode); 10 min maximum (peak mode)
Timer: quartz crystalEvent markers: two delay relay
contact outputs—one fractionation marker and one row marker (100 millisecond minimum each)
Programmable fraction range Time: 1 sec to 99 min, 59 sec Drop count: 1 to 9999 drops External count: 1 to 9999 counts
Programmable wait drain range Time: 1 sec to 99 min, 59 sec Drop count: 1 to 9999 drops External count: 1 to 9999 counts
Drop rate: 10 drops/sec maximumUser memory: 10 programs, with
battery back-up Operating temperature: 36 to 86°F
(2 to 30°C) without condensationHeater: 15 wattsPower: 90 to 260 VAC; 50/60 HzDimensions:
143⁄4"W x 101⁄4"H x 143⁄8"D (375 mm x 260 mm x 365 mm)
Specifications & Ordering Information
Programmable Fraction CollectorStore up to 10 different procedures
Ideal for automated liquid chromatography
systems.
01473-90
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Liquid Chromatography Columns
Catalog number Packing Column PriceKH-25583-34 Waters
C18 150 x 4.6 KH-25585-00 KH-25585-02 C18 250 x 4.6 KH-25585-04 KH-25585-06
Waters Spherisorb®
ODS-1 250 x 4.6 KH-25583-24 KH-25582-24 ODS-2 150 x 4.6 KH-25583-30 KH-25582-30 ODS-2 250 x 4.6 KH-25583-32 KH-25582-32 Cyano 150 x 4.6 KH-25583-70 KH-25582-70 Cyano 250 x 4.6 KH-25583-72 KH-25582-72
GL Sciences Inertsil®
ODS-2 150 x 4.6 KH-25585-08 KH-25585-10 ODS-2 250 x 4.6 KH-25585-12 KH-25585-14 Phenyl 150 x 4.6 KH-25585-16 KH-25585-18
C8 150 x 4.6 KH-25585-20 KH-25585-22 AKZO Nobel Kromasil®
C18 250 x 4.6 KH-25585-24 KH-25585-26 Merck LiChrosorb®
RP-18 250 x 4.6 KH-25585-28 KH-25585-30 RP-8 KH-25585-32 KH-25585-34
Merck LiChrospher®
RP-18 250 x 4.6 KH-25585-36 KH-25585-38 RP-18* KH-25585-40 KH-25585-42
*Endcapped.
Specifications & Ordering InformationMaterial: 316 SS, PEEKParticle size: 3 µmDimensions: 53 mm L x 7 mm ID
Hamilton polymeric PRP™ columns are resin-based, offering excellent resolution due to their high selectivity, efficiency, and capacity. The chemically inert macroporous poly (styrene-divinylbenzene) stationary phase is stable from pH 1 to 13. Compatible with any concentration of organic solvent; can be used up to 60°C and 5000 psi. Hamilton guarantees that the spherical media will not shrink or swell.
PRP-1 Reverse Phase Columns contain poly (styrene-divinyl-benzene). Large surface area (415 m2/g) allows high capacity and rapid flow rates for fast analysis. Ideal for separating strong acids and bases. Efficiency of 30,000 to 50,000 plates/meter.
PRP-3 Reverse Phase Columns are made of a wide pore (300 Å) poly (styrene-divinylbenzene) copolymer. Use to isolate or purify proteins and peptides. For extended life, regenerate your column up to five times—protein recoveries are better than 90%. Choose a 50-mm column for the gradient elution of high-molecular-weight proteins. The 150-mm column is best suited for the gradient separation of lower molecular weight proteins (less than 5000 MW).
PRP-X100 Strong Anion Exchange Columns contain poly (styrene-divinylbenzene)-trimethyl ammonium. These columns offer superior, rapid resolution. Exchange capacity is 0.2 mEq/g.
PRP-X200 Strong Cation Exchange Columns contain poly (styrene-divinylbenzene) sulfonate. These columns also offer superior, rapid resolution; exchange capacity is 0.035 mEq/g.
PRP-X300 Ion Exclusion Columns contain a partially sulfonated poly (styrene-divinylbenzene) media. Exhibit a high degree of both reverse phase and ion exclusion characteristics; separations are based on charge as well as relative hydrophobicity. Ideal for analysis of carboxylic acids and alcohols—no elevated temperature required. Exchange capacity is 0.17 mEq/g.
Several varieties—select the column that best fits your application
Hamilton pH-Stable HPLC ColumnsEfficient separations with durable media
Catalognumber Application Particle
sizeDimensionsL x ID (mm) Price
PRP-1 reverse phase columnsKH-01941-00 High resolution 5 µ 150 x 4.1 KH-01941-02 Fast analytical 10 µ 150 x 4.1 KH-01941-04 Analytical 10 µ 250 x 4.1 KH-01941-06 Semipreparative 10 µ 305 x 7.0 KH-01941-08 Preparative 10 µ 250 x 21.5 PRP-3 reverse phase columnsKH-02004-00 Protein isolation 10 µ 50 x 4.1 KH-02004-10 Protein isolation 10 µ 150 x 4.1 PRP-X100 strong anion exchange columnsKH-01942-00 Fast screening 10 µ 100 x 4.1 KH-01942-02 Fast analytical 10 µ 150 x 4.1 KH-01942-04 Analytical 10 µ 250 x 4.1 PRP-X200 strong cation exchange columnsKH-01943-00 Fast analytical 10 µ 150 x 4.1 KH-01943-02 Analytical 10 µ 250 x 4.1 PRP-X300 ion exclusion columnsKH-01944-00 Fast analytical 7 µ 150 x 4.1 KH-01944-02 Analytical 7 µ 250 x 4.1
Specifications & Ordering Information
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Eppendorf® Column Temperature Control SystemIncrease the accuracy of your results by ensuring a stable temperature
42650-00
42650-60
Eppendorf® Column HeaterThis Eppendorf column heater heats up to-two columns at a time and accepts columns from 1⁄4" to 1⁄2" OD and 30-cm length. Includes inlet preheater to warm the mobile phase before it reaches the column—eliminates the problem of column temperature gradients and offers greater efficiency. Position heater vertically to conserve space.
What’s included: inlet preheater and support brackets.
Eppendorf® Temperature ControllerRegulate your column heater with this Eppendorf temperature controller. Controller controls from ambient to 150°C—with a precise ±0.1°C temperature stability. Ten-turn potentiometer lets you set temperature in 0.1°C increments. The 4-digit LED lets you monitor the set or current temperature of the column. Controller also offers ON/OFF remote operation.
What’s included: a-6-ft cord with plug.
Cat. no. KH-42650-00 KH-42650-05Columns Maximum of twoColumn sizes 1⁄4" to 1⁄2" OD x 30 cm LInlet preheater Two sets (included)Heater element 125 wattsHeater block Aluminum, two part ovenSensor 1000 Ω Pt RTDThermal cutout 155°C (±5°C) temperature limitPower (50/60 Hz) 110 VAC 220 VACDimensions 191⁄2"L x 23⁄4"W x 3"HPrice
Cat. no. KH-42650-60 KH-42650-65Range Ambient to 150.0°CResolution 0.1°C incrementsStability ±0.1°C typical core stabilityAccuracy ±1°C at 50°CDisplay 4-digit LEDPower (50/60 Hz) 110 VAC 220 VACDimensions 81⁄2"L x 7"W x 31⁄4"HPrice
Easily change the configuration of your system with these self-adjusting sample loops. Loops provide a variety of inert, PEEK tubing connection options. All models are 1⁄16" OD.
PEEK Sample Loops
02015-00
Catalog number Color ID Length Volume PriceKH-02015-00 Red 0.005" 10 cm 1 µL KH-02015-01 20 cm 3 µL KH-02015-10 Blue 0.010" 10 cm 5 µL KH-02015-11 20 cm 10 µL KH-02015-20 Orange 0.020" 10 cm 20 µL KH-02015-21 20 cm 40 µL KH-02015-30 Green 0.030" 10 cm 46 µL KH-02015-31 20 cm 91 µL
Easy-to-use guard cartridges trap unwanted particulates and dissolved impurities before they reach your analytical HPLC column.
Choose cartridges with the same packing media as in your HPLC column. Guard cartridge kits come complete with two cartridges and one holder. Guard cartridges accept 1⁄16" OD tubing. Replacement cartridges are sold in packs of five. Order guard cartridge kits and replacement cartridges from table below.
Catalognumber
Max psi
Maxtemperature
Dimensions(OD x ID) Color Qty/
pkPrice/
pk316 Stainless steel tubingKH-03300-02 3000
550°F (288°C)
1⁄16" x 0.032"
— Two 3-ftlengths
KH-03300-05 3000 1⁄8" x 0.055" KH-03300-10 3000 1⁄4" x 0.180" KH-03300-15 3000 3⁄8" x 0.305" KH-03300-20 3000 1⁄2" x 0.380" Tefzel® tubingKH-06488-01 975
302°F (150°C)1⁄8" x 0.063"
— 10 ft *
KH-06488-02 975 1⁄16" x 0.032" *KH-06488-03 1514 1⁄16" x 0.016" *PEEK (polyetheretherketone) tubingKH-02006-00 5000
212°F (100°C)
1⁄16" x 0.010" Natural
5 ft
KH-02006-10 5000 1⁄16" x 0.010" Blue KH-02006-20 5000 1⁄16" x 0.020" Orange KH-02006-30 3000 1⁄16" x 0.030" Green
Chromatography Tubing316 Stainless Steel Tubing offers good chemical resistance.
Tefzel® Tubing is translucent and slightly flexible. Ideal for biochromatography—will not denature sensitive biomolecules. Tefzel exhibits excellent mechanical resistance and is more chemically inert than SS tubing.
PEEK Tubing is flexible and easy to cut; provides excellent chemical resistance to both organic and inorganic solvents.
AccessoriesEG-06438-10 Tubing cutter, cuts plastic tubing from 1⁄16 to 5⁄8" OD EG-06438-11 Replacement blade
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Upchurch Scientific® Chromatography Fittings
A-E Stainless Steel Fittings include nuts and ferrules. They are equivalent to Parker and Swagelok®. Tees and crosses connect two or more columns from the injector (0.020" bore), and join multiple solvent lines before the injector (0.040" bore). Tees and crosses take 1⁄16" OD tubing. Tee includes three ferrules and nuts each. Cross includes four ferrules and nuts each. Zero dead-volume (ZDV) unions connect tubing without any dead volume.
F Standard Fingertight® I High-Pressure Fittings are in one piece, and are finger tightened to withstand 4000 psi. Use with 1⁄16" OD plastic or SS tubing.
G-H Standard Fingertight II High-Pressure Fittings hold up to 6000 psi. Wrench tighten the SS nut/PEEK* ferrule type to hold up to 10,000 psi. Use with 1⁄16" OD plastic or SS tubing.
I PEEK* Fingertight III Fittings withstand bursting pressures of any 1⁄16" OD tubing or to 6000 psi. Replacement ferrules available below right.
J-L PEEK* High-Pressure Unions, Tees, and Crosses hold up to 5000 psi and include Fingertight III fittings. Fittings work with any 1⁄16" OD tubing.
J-L PEEK* Low-Pressure Unions, Tees, and Crosses hold up to 1000 psi (1⁄16" tubing), or to 500 psi (1⁄8" tubing). PEEK flangeless fittings included. Models 02008-00, -10, and -20 take 1⁄16" OD tubing; models 02008-04, -14, and -24 take 1⁄8" OD tubing.
M PEEK* Fitting Kit contains twenty ferrules, six Fingertight III nuts with ferrules, two metric adapters, two 10-32 female to 1⁄4-28 female adapters, two unions with 0.020" bore, one union with 0.050" bore, tees with 0.020" bore and 0.050" bore each, and crosses with 0.020" and 0.050" bore each.
N-O Flangeless Fittings are easy to assemble; slide the ferrule over the tubing and finger tighten the nut. No need to flange your Teflon® tubing. Models 02007-54 and -64 take 1⁄16" OD tubing and are rated at 1000 psi; models 02007-56 and -66 take 1⁄8" OD tubing and are rated at 500 psi. Feature 1⁄4-28 threads.
P LiteTouch® Ferrule Systems feature unique double-coned ferrule and SS compression ring. Won’t twist tubing while disconnecting. Compression ring remains attached to tubing for quick reconnection. Rated for 5000 psi (01939-36) or 7000 psi (01939-34 and -44). Order tool 01939-39 (at right) to remove compression ring. Order SS nuts (“A”) or PEEK nuts (“P”) in table.
Q Column Couplers allow zero dead-volume connections between column and column, column and detector, or column and guard column. Include a 5-cm length of 0.007" ID tubing.
*Maximum operating temperature is 302°F (150°C).
A B
C
D
E
F G H
I J
K
L M
N O P
Q
AccessoriesKH-02007-50 Replacement ferrule for “I” fitting 02007-00 KH-02007-52 Replacement ferrule for “I” fitting 02007-02 KH-01939-39 Removal tool for “P” ferrule systems
Key Cat. no. Description Qty PriceStainless steel fittingsA KH-01939-80 1⁄16", male nut Pk of 10 B KH-01939-90 1⁄16", ferrule Pk of 10
C KH-01939-50 0.020" bore, tee0.040" bore, tee
11
KH-01939-52
D KH-01939-60 0.020" bore, cross0.040" bore, cross
M KH-02008-52 PEEK fitting kit 1 Flangeless fittings
N KH-02007-54 1⁄16", Tefzel ferrule Pk of 10 KH-02007-56 1⁄8", Tefzel ferrule Pk of 10
O KH-02007-64 1⁄16", PEEK male nut Pk of 10 KH-02007-66 1⁄8", PEEK male nut Pk of 10
LiteTouch® ferrule systems
P
KH-01939-34 1⁄16", PEEK ferrule system Pk of 10 KH-01939-36 1⁄8", PEEK ferrule system Pk of 10 KH-01939-44 1⁄16", PEEK nut Pk of 10 KH-01939-46 1⁄8", PEEK nut Pk of 10
Column couplers
Q KH-01939-95 Two CTFE fittings, built-in nuts and ferrules 1 KH-01939-96 Two sets of acetal nuts/ketone ferrules 1
Specifications & Ordering Information
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Switching Valves offer two flow patterns. Carbon-filled PEEK valve, flangeless Tefzel® ferrules, and Delrin® nuts.
Drain Valves release pressures from HPLC systems or redirect the flow of the mobile phase. Carbon-filled PEEK valve includes three Fingertight® III fittings and a titanium rotor. Dead volume is 6.0 µL when closed and 18.4 µL when open.
Shut-Off Valves. PEEK valve and nut, flangeless Tefzel ferrule.
Upchurch® HPLC Valves
Catalog number Description Max
psi Tubing Price
KH-02012-02 Switching valve, three port 500 1⁄16" OD KH-02012-00 Switching valve, four port KH-02013-00 Drain 5000 1⁄16" OD KH-02013-01 Drain, bulkhead mountable KH-02014-00 Shut-off valve 500
1⁄16" OD KH-02014-01 1⁄8" OD
02012-00 02013-00 02014-00
25585-66
Metal-Free Solvent Inlet FilterThis 20-µm polyethylene filter is housed in a PEEK plastic body. Filter features a 1⁄4-28 UNF thread type, can be used with 1⁄16" or 1⁄8" OD tubing, and can withstand temperatures up to 212°F (100°C).
KH-42701-00 Disposable filter, for 1⁄16" ID tubing — —KH-42701-02 Disposable filter, for 1⁄8" OD tubing — —KH-42700-00 Replaceable filter assembly, 2 µm KH-42700-50 KH-42700-02 Replaceable filter assembly, 10 µm KH-42700-52
Disposable Solvent Inlet Filters are economical and provide up to 6 months of protection (depending on usage and type of mobile phase). These 10-µm filters are easy to replace; simply tighten by hand. Wetted parts are 316 SS. Max flow rate is 15 mL/min; stem OD is 1⁄16".
Replaceable Solvent Inlet Filters are designed with the inlet tube at the bottom of the filter allowing filters to draw solvent to within 0.06" of the reservoir bottom. Reusable filter assembly features replaceable filter cup—easily change by loosening lock nut and removing cup. Wetted parts are 316 SS cup, PEEK nut and Tefzel seal. Max flow rate is 10 mL/min. Use with 1⁄8" or 3⁄16" OD tubing.
Upchurch® HPLC Solvent Filters
42701-00
42700-00
Solvent Filters
Whatman® In-Line Solvent DegassersDegassers simultaneously filter and degas solvents to provide better HPLC reproducibility. They also eliminate false peaks due to solvent impurities and gas bubbles. Degassers feature built-in 0.2-µm filter. Aqueous phase degasser has a nylon filter and organic phase degasser has a polypropylene filter. Other wetted parts are polypropylene. Each box includes one set of ferrule nuts for 1⁄8" OD tubing. 29702-00
Metal-Free In-Line FilterIn-line filters are placed between the injection valve and the column. PEEK body and PEEK Alloyed with PTFE (PAT®) filter construction ensure that only inert, high-strength polymers contact the fluid stream. Max pressure is 5000 psig. Use with 1⁄16" OD tubing.
Upchurch® HPLC In-Line FiltersInlet and outlet connections fit Upchurch, Swagelok®, Valco and Parker fittings. These stainless steel filters have a stainless steel frit and PEEK ring.
In-Line Solvent Filter is placed between pump and sample injection valve to trap piston seal particles. An 8° distribution cone spreads the solvent flow over the entire surface of the frit (0.094" dia x 0.062"), with 0.050" dia flow thru-hole for solvent.
Precolumn Filters have 0.020" dia flow thru-holes and 8° distribution cones for minimum band spreading and mixing.
In-Line Filters
239
See page 239 for PEEK (polyetheretherketone) and Tefzel® chromatography tubing.
CH Chromatography
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HPLC
Use this bottle cap to change tubing without unscrewing fittings. Cap comes with Tefzel® fittings and an adapter to accommodate either 1⁄8" or 5⁄32" OD tubing. Screw cap size is 45 mm; see our “Bottles” section on pages 144–166. Note: Do not use caps in applications exceeding 10 psi.
Quick-Disconnect Bottle Cap
Catalog number Description PriceEK-02016-00 Quick-disconnect bottle cap
Labglass™ Filtration AssembliesLabglass™ filtration assemblies are designed for HPLC, particulate, and microbiological contamination filtrations. Sintered glass support provides even, flat support structure for membrane filters. Assemblies include a coarse fritted glass support base, a graduated glass funnel, an anodized aluminum clamp, and a #8 silicone stopper with 9⁄16" hole.
Order the Labglass Pyrex® glass filtering flasks separately below. Then attach a 3⁄8" ID vacuum hose to the sidearm of the flask via the #2 hose connector.
See our selection of membrane filters on page 566.
Labglass Filtering FlasksCatalog number Capacity Dimensions (in mm) Price
EK-34509-32 1000 mL 225 H x 135 base diaEK-34509-34 2000 mL 292 H x 168 base diaEK-34509-36 4000 mL 370 H x 208 base dia
Catalognumber
Filtersize
Funnelvolume Price
Replacement funnelCatalog number Price
EK-34509-00 47 mm 300 mL KH-34509-38EK-34509-10 90 mm 1000 mL KH-34509-40
Filtration assembly
includes base, funnel, clamp, and stopper.
Order the filtering flask
separately.
02017-45
02017-60
Kontes HPLC Mobile Phase Reservoirs
Ultra-Ware® HPLC Economy Cap SystemsThese complete reservoir systems have chemical-resistant fluid/vapor contact surfaces. Cap body is PTFE; with TFE/-propylene O-ring and polypropylene screw collar. Tubing passes through 1⁄4-28 fittings. Cap has two 1⁄8" through holes and one through hole for 1⁄6" OD tubing. Flat-bottom reservoir is Type I, Class A borosilicate glass with a GL 45-4 screw thread and a plastic safety coating. The plastic coating blocks UV light up to 385 nm.
What’s included: one 2 mm PEEK inlet/sparge filter, two ETFE fittings, one ETFE plug, 10 ft of 1⁄8" OD Teflon® FEP tubing, and reservoir.
Catalog number Reservoir volume PriceKH-02017-45 1 L KH-02017-50 2 L KH-02017-55 5 L
Specifications & Ordering Information
Ultra-Ware HPLC Standard Cap SystemsUse these complete mobile phase systems for solvent handling integrity, convenience, and efficiency. Borosilicate glass and PTFE construction provide excellent chemical resistance to most HPLC mobile phases. Make convenient line connections without handling inside tubing and sparge filter. Connecting threads on top and bottom of the cap use 1⁄4-28 flangeless fittings.
Cap body is PTFE; O-ring is TFE/propylene. Contact our Applications Specialists for a Kalrez O-ring to use with aggressive organic solutions or a filtration cap and solvent pickup adapter for direct filtration into reservoir. Plastic-coated conical bottom reservoir enables maximum mobile phase utilization; top has GL 45-4 screw thread.
What’s included: three hole cap, standard fittings kit, Teflon FEP tubing, and reservoir.
Specifications & Ordering Information
Mobile Phase ReservoirsThese reservoirs are manufactured of high-quality borosilicate glass for maximum chemical resistance. Reservoirs are coated with plastic for safety and UV resistance. Select from conical-bottom configuration for maximum solvent utilization or flat-bottom reservoirs.
Catalognumber
Bottomstyle
Reservoirvolume Price
KH-02017-00
Conical
0.5 L KH-02017-05 1 L KH-02017-10 2 L KH-02017-15 5 L KH-02017-20
Flat1 L
KH-02017-25 2 L KH-02017-30 5 L
Specifications & Ordering Information
1205
See page 1205 for gas regulators.
Catalog number Reservoir volume PriceKH-02017-60 1 L KH-02017-65 2 L KH-02017-70 5 L
For our complete selection of test tubes.
1842–1844
CHChromatography
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LG
AClean-up/drying
columns
Columns with fritted disk,
24/40 joint, stopcock, and plug
BLG
Columns with reservoirs, 24/40 joint,
stopcock, and plug
C
Glass Chromatography ColumnsIdeal for adsorption, gel, permeation, and ion exchange techniques
A Disposable Glass Columns are ideal for the clean up of sample extracts or for drying of samples. Disposable columns reduce the chances of cross contamination.
Specifications & Ordering Information
Cat. no. Capacity Dimensions (mm) Qty/cs Price/csKH-34695-60 10 mL 10.9 OD x 8.60 ID x 290 H 200 KH-34695-62 25 mL 15.8 OD x 14.0 ID x 297 H 100 KH-34695-64 50 mL 18.0 OD x 16.2 ID x 300 H 50
C Columns with Reservoirs are perfect for flash chromatography applications. Includes a PTFE plug to control flow rate.
Specifications & Ordering Information
Cat. no. Capacity Dimensions (mm) Qty/cs Price/csKH-34695-54 200 mL 10.5 ID x 250 L 1 KH-34695-56 250 mL 19.0 ID x 300 L
B Standard Glass Columns are made of borosilicate glass and are available with or without joints and fritted disks. The joints on top of columns permit the connection of a funnel or other reservoir. Fritted disks (40 to 60 µm porosity) support packing materials. Includes a PTFE plug to control flow rate.
Specifications & Ordering Information
Catalognumber
Fritteddisk
joint24/40
Dimensions(mm)
Qty/cs
Price/ cs
KH-34695-46
No No
10.5 ID x 150 L
1
KH-34695-48 10.5 ID x 250 L KH-34695-50 19.0 ID x 250 L KH-34695-52 19.0 ID x 500 L KH-34695-32
No Yes
10.5 ID x 150 L
1
KH-34695-34 10.5 ID x 250 L KH-34695-36 19.0 ID x 250 L KH-34695-38 19.0 ID x 500 L KH-34695-40 10.5 ID x 150 L KH-34695-42 Yes Yes 10.5 ID x 250 L 1 KH-34695-44 19 ID x 250 L
Glass WoolEasy-to-use wool is made of fine glass fibers. Each bag contains one piece approximately 2" in diameter and 22" long.
KH-34552-01 Glass wool. Pack of 1 lbKH-34552-21 Glass wool. Case of twelve 1-lb packs
Low-Pressure Liquid Chromatography FittingsChemically inert PEEK construction These flangeless fittings connect easily by hand and offer an economical alternative to stainless steel fittings. Select from individual fittings or the comprehensive kit. Low-pressure fittings are rated to 1000 psi for 1⁄16" tubing and 500 psi for 1⁄8" tubing.
A-C Low-Pressure Unions, Tees, and Crosses – PEEK flangeless 1⁄4-28 threaded fittings included. 1⁄8" fittings feature 0.050" bore and are rated to 500 psi; 1⁄16" fittings feature 0.020" bore and are rated to 1000 psi.
D-E Flangeless Fittings are easy to assemble. Slide the ferrule over the tubing and finger-tighten the nut. Excellent chemical resistance. 1⁄8" fittings are rated to 500 psi; 1⁄16" fittings are rated to 1000 psi. All fittings feature 1⁄4-28 threads.
F PEEK Fitting Kit contains convenient 1⁄4-28 threaded fittings for both 1⁄16" and 1⁄8" tubing:1⁄16" tubing: PEEK flangeless nuts (6), Tefzel® flangeless ferrules (10), and PEEK 0.020" bore Tee (1), cross (1), and union (2). Also includes 10-32 to M6 adapters (2) and 10-32 to 1⁄4-28 female adapters (2).1⁄8" tubing: PEEK flangeless nuts (6), Tefzel flangeless ferrules (10), and PEEK 0.050" bore Tee (1), cross (1), and union (2). Also includes 10-32 to M6 adapters (2) and 10-32 to 1⁄4-28 female adapters (2).
Contact our Application Specialists for information regarding Delrin® acetal fittings or other materials.
Key Description QtyFor 1⁄16" tubing For 1⁄8" tubing
Catalog number Price Catalog number PriceA Union, PEEK 1 KH-02008-00* KH-02008-04** B Tee, PEEK 1 KH-02008-10* KH-02008-14** C Cross, PEEK 1 KH-02008-20* KH-02008-24** D Nut, PEEK 10 KH-02007-64 KH-02007-66 E Ferrule, Tefzel 10 KH-02007-54 KH-02007-56 F PEEK fitting kit 1 KH-02008-52 KH-02008-52
*0.020" bore. **0.050" bore.
Specifications & Ordering Information
A
B
C
D
E
F 02008-52
02008-00
02008-10
02008-20 01939-30
02007-66
Liquid
CH Chromatography
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Standard column 34696-02 shown with flow adapter 34696-54
Standard column 34696-02
Column design minimizes dead-volumeSpectrum chromatography columns are made from premium borosilicate glass. Each column is constructed with non- clogging bed supports made of a thin, 10-µm membrane and a 50-µm woven grid. All columns are autoclavable.
Columns for Aqueous Solutions include Teflon®, polypropylene, borosilicate glass, and nitrile rubber wetted parts. Ideal for most aqueous solutions—including most acids, bases, and salts.
Columns for Organic Solutions provide excellent chemical resistance with only Teflon and borosilicate glass wetted parts. Ideal for use with solvents (except hydrofluoric acid-solutions).
Accessories
Adjustable PlungersPlungers are adaptable bed supports designed to reduce the bed height of a column. Use at either or both ends of the column when you do not want space at the top of the packing. Plungers use the same non-clogging mesh bed support as the columns.
Plungers for aqueous solutions have polypropylene, nylon, Teflon, and Viton® wetted materials. Plungers for organic solutions have only Teflon wetted materials for ultimate chemical resistance.
Specifications & Ordering Information for Columns
Dia Length Maxpressure Volume Total
volumeColumns for aqueous solutions Columns for organic solutions
Catalog number Tubing OD Price Catalog number Tubing OD Price
0.9 cm 15 cm 50 psi 0.6 mL/cm 9.4 mL KH-34697-00 3⁄16" KH-34697-06 1⁄8" 30 cm 19 mL KH-34697-02 KH-34697-08
1.5 cm 20 cm 45 psi 1.7 mL/cm 35 mL KH-34697-12 3⁄16" KH-34697-20 1⁄8" 30 cm 53 mL KH-34697-14 KH-34697-22 20 cm 98 mL KH-34697-28 KH-34697-36
2.5 cm 30 cm 40 psi 4.9 mL/cm 147 mL KH-34697-30 3⁄16" KH-34697-38 1⁄8" 60 cm 294 mL KH-34697-32 KH-34697-40 30 cm 588 mL KH-34697-44 3⁄16" KH-34697-50 1⁄8"
5.0 cm 60 cm 25 psi 19.6 mL/cm 1176 mL KH-34697-46 3⁄16" KH-34697-52 1⁄8" 100 cm 1960 mL KH-34697-48 1⁄4" KH-34697-54 1⁄4"
Spectrum Chromatography Columns
Column ID Adjustmentlength
For aqueous solutions For organic solutionsCat. no. Price Cat. no. Price
0.9 cm 10 cm KH-34697-56 KH-34697-58 1.5 cm 10 cm KH-34697-60 KH-34697-62 2.5 cm 16 cm KH-34697-64 KH-34697-66 5.0 cm 32 cm KH-34697-68 KH-34697-70
Chromaflex® Chromatography Columns have borosilicate glass construction with PTFE end fittings and HDPE bed supports. Each end fitting has a thin PTFE layer that is curled over the O-ring to form a solvent resistant, leak free seal. These columns can be-used with all aqueous mobile phases. To use with organic solvents, order optional 20-µm PTFE bed supports from table.
What’s included: each column is supplied with two PTFE end fittings with 20-µm porosity HDPE bed supports, 5-ft (1.5 m) PTFE tubing, two flangeless tubing nuts, and two flangeless ferrules.
Flow Adapters protect gel bed from disruption during sample loading and eliminates dead volume by delivering the sample and buffer directly to top of gel bed. Ideal for concentration and pH gradients, reverse flow separations, and high-resolution gravity flow.
What’s included: 20-µm porosity HDPE bed support, PTFE/propylene O-ring seal, stainless steel adjusting rod, PTFE body and 5-ft (1.5 m) FEP Teflon® tubing.
Sterilization: ethylene oxide, 2N NaOH or 100% ethanol (autoclaving is not recommended)
ID Length Maximumvolume
Maximumpressure
OD ofsuppliedtubing
Standard columns Flow adapters PTFE bed supportCatalognumber Price Catalog
number Price Catalognumber Price
1.0 cm
30 cm 24 mL100 psi
(6.9 bar) 1⁄16"
KH-34696-00
KH-34696-54 KH-34696-48 60 cm 47 mL KH-34696-02 100 cm 79 mL KH-34696-04 120 cm 95 mL KH-34696-06
2.5 cm — — — 1⁄16" — — KH-34696-56 — —
2.5 cm
30 cm 147 mL75 psi
(5.2 bar) 1⁄8"
KH-34696-08
KH-34696-58 KH-34696-50 60 cm 295 mL KH-34696-10 100 cm 491 mL KH-34696-12 120 cm 589 mL KH-34696-14
4.8 cm
30 cm 543 mL50 psi
(3.4 bar)1⁄8"
KH-34696-16
KH-34696-60 KH-34696-52 60 cm 1086 mL KH-34696-18 100 cm 1810 mL KH-34696-20 120 cm 2172 mL KH-34696-22
Chromaflex® Standard Chromatography ColumnsPTFE shielded O-rings form a solvent resistant, leak-free seal
AccessoriesKH-34696-70 Adapter, 1⁄4-28 thread male to 1⁄16" barb KH-34696-72 Adapter, 1⁄4-28 thread male to 1⁄8" barb
34697-00
Liquid
CHChromatography
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SPE CartridgesRemove sample impurities the fast, economical wayUse these versatile SPE (solid-phase extraction) cartridges to get rid of liquid sample impurities with minimal effort. Eliminate the excess time, hassle, glassware, solvent usage, and cleaning associated with conventional liquid-liquid extraction. SPE cartridges are also widely used to easily concentrate analytes, e.g. trace organic pollutants in environmental samples.
Flanged polypropylene cartridges contain highest quality silica media. Low levels of fines and acid washing reduce or eliminate the need to pre-condition Cole-Parmer SPE beds. Select the media and loading suitable for your application.
Solid-phase extraction or ”SPE” is a form of “digital chromatography”, an all-or-nothing approach to purifying a sample. Unlike regular liquid chromatography, where the elution of peaks or components occurs gradually, molecules adhere to SPE completely and then are totally released from the SPE media in a separate step.
Total adsorption of an analyte to a sorbent enables impurities to be rinsed away from the supported material using an appropriate solvent. For this step, the solvent has substantially less affinity for the analyte than does the SPE support material.
A final elution is then used to release the now-purified analyte away from the support. In this step, the solvent used has a strong affinity for the analyte. The process is shown visually at right.
1. Condition sorbent
2. Apply sample and analyte
Eluted interferences
Analyte
3. Interference elution
4. Analyte elution
10937-00
Call our Application Specialists for
additional information on available SPE media and sizing.
Application-specific bonded functionalities are also available with
technical support.
847-549-7600
ColeParmer.com/ChromatographyEven MORE online! Look for new items, stock status, technical info, etc.
Polar nonionic (normal phase)KH-10937-00 Silica 500 mg — — 3 mL 6.3 x 6 cm 50 KH-10937-03 1000 mg 6 mL 6.5 x 10 cm KH-10937-30 Si-cyano 500 mg 7% — 3 mL 6.3 x 6 cm 50 KH-10937-33 1000 mg 6 mL 6.5 x 10 cm
Nonpolar (reversed phase)KH-10937-12 C18 500 mg 17% Yes 3 mL 6.3 x 6 cm 50 KH-10937-15 1000 mg 6 mL 6.5 x 10 cm KH-10937-06 C18, water wettable 500 mg 17% No 3 mL 6.3 x 6 cm 50 KH-10937-09 1000 mg 6 mL 6.5 x 10 cm KH-10937-18 C8 500 mg 12% Yes 3 mL 6.3 x 6 cm 50 KH-10937-21 1000 mg 6 mL 6.5 x 10 cm KH-10937-24 C8, water wettable 500 mg 12% No 3 mL 6.3 x 6 cm 50 KH-10937-27 1000 mg 6 mL 6.5 x 10 cm
Polar ionic (ion exchange)KH-10937-36 Si-propylsulfonic acid 500 mg 12.3% as acid — 3 mL 6.3 x 6 cm 50 KH-10937-39 1000 mg 6 mL 6.5 x 10 cm KH-10937-42 Si-TMA chloride 500 mg 14% — 3 mL 6.3 x 6 cm 50 KH-10937-45 1000 mg 6 mL 6.5 x 10 cm KH-10937-48 SCX/SAX 500 mg — — 3 mL 6.3 x 6 cm 50 KH-10937-51 1000 mg 6 mL 6.5 x 10 cm
SPE
CH Chromatography
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Specifications & Ordering Information
Preparative TLC Uniplates
Analytical TLC Uniplates
AccessoriesKH-34105-35 Adsorbent scraper, 13 mm KH-34105-37 Replacement scraper blades, 13 mm. Pack of five
34101-40 34101-00
Efficient separations—economically!These TLC plates combine top-quality adsorbents, consistent manufacturing, rigid QC, and careful packaging— the results of 40 years of experience dedicated to thin layer techniques. Select the perfect plates for your application—analytical or preparative. Choose plates with or without a fluorescent indicator. Scored plates snap apart evenly to facilitate single-use separations or screening.
Analytical TLC UNIPLATESSoft Layer Plates use E. Merck silica gel and CaSO4 binder for general organic separations. Not recommended for solvent systems >20% water.
Hard Layer Plates with Organic Binder use proprietary silica to enable separations using solvent media up to 80% water.
Hard Layer Plates with Inorganic Binder have a hard proprietary silica layer and are recommended for charring visualization applications.
Reversed Phase Plates have a non-polar adsorbent—hydrocarbon-impregnated silica.
HPTLC Plates achieve fast, high-resolution separations using proprietary silica. Recommended for sample sizes <1 µL.
Preparative TLC UNIPLATESSoft Layer Plates use E. Merck silica gel and CaSO4 binder for general organic separations and easy scraping.
Reversed Phase Plates use a readily removed non-polar adsorbent—hydrocarbon-impregnated silica.
Analtech Glass-backed TLC UNIPLATES™
Precision-bore capillary tubes are calibrated to contain when filled from end to end. Insert a tube into the reusable dispensing bulb assembly and capillary action draws fluid up to the full capacity. Simply squeeze the bulb to dispense. Each case contains one bulb and 100 disposable tubes.
10 x 20 — KH-34102-50 KH-34102-55 8 KH-34102-60 KH-34102-65
HPTLC, Silica Gel HP-HL, 150 µm thickness, 10 µm particle, 60 Å pore, organic binder10 x 10 — 25 KH-34103-50 KH-34103-55 10 x 20 8 KH-34103-60 KH-34103-65
Thin Layer
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Whatman® Flexible Thin Layer Chromatography (TLC) PlatesMake the TLC plate you need—with a pair of scissors!These plates are ideal for applications that require rapid sample isolation or elution before other tests can be performed. Flexible plates exhibit the same characteristics as glass backed, silica gel plates while offering the convenience of custom sizing and flexibility.
MediaSilica Gel 60 Å Flexible Plates exhibit similar selectivity to the glass backed plates and are widely applicable for moderately to strongly polar analytes. Available on aluminum or polyester backing material.
Ion Exchange Plate (DEAE-diethylaminoethyl tertiary amine) is used for anionic species and is available on polyester-backed material.
Backing MaterialAluminum-Backed Plates are particularly useful for applications requiring charring.
Polyester-Backed Plates can be heated up to 110°C and are compatible with mobile phases containing strong acids or bases.
Cut flexible TLC plates to any size.
Specifications & Ordering Information
*Three-centimeter C18 strip on silica gel layer.
Catalognumber Media Backing
materialLayers
thicknessPlate size
(cm)Fluorescent
indicatorQty/bx
Price/bx
KH-34100-24 Silica gel 60 Å Polyester 250 µm
20 x 20
No
25
KH-34100-26 Silica gel 60 Å Polyester 250 µm Yes KH-34100-28 Silica gel 60 Å Aluminum 250 µm No KH-34100-30 Silica gel 60 Å Aluminum 250 µm Yes KH-34100-32 Ion exchange Polyester 100 µm 20 x 20 No 25
For our complete selection of “Ultraviolet Equipment.”
1902 to 1913
Whatman® Chromatography and Blotting PapersHighest purity of cellulose with no additives
KH-34100-00 1 0.18 130 20 x 20 100 KH-34100-06 46 x 57 KH-34100-10 3 0.36 130 46 x 57 100 KH-34100-04 3 MM 0.34 130 20 x 20 100 KH-34100-12 46 x 57
Thin Papers are ideal when the solute is not heavily loaded. They give you the best combination of speed and resolution for general purpose separations.
Thick Papers work best for heavily loaded solutes. Preparative separations benefit from the increased sample volumes that thicker papers carry. Ideal for-electrophoresis techniques.
Grade 1 CHR Papers are one of the thinnest papers available. They provide moderate flow rate, smooth surface, and good resolution.
Grade 3 CHR Papers are fairly thick and are ideal for general applications with medium to heavy solute loadings.
Grade 3 MM CHR Papers are thick with a smooth, medium-wet surface. These papers are used extensively as blotting paper for both electrophoresis and general chromatography.
For a full selection of our transilluminators.
869 to 879
Thin Layer
CH Chromatography
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Glass TLC Developing TanksTanks accommodate standard TLC plates. The top surface is ground to a precise tolerance for tight sealing to enable tank saturation and optimum plate development. Thinline™ tanks accommodate two plates and require less solvent for development. Latch-Lid™ units hold lids in place with stainless steel latches. Order rack to develop maximum number of plates in a tank.
Thin Layer Chromatography (TLC) Accessories
Specifications & Ordering Information
Wheaton Chromatography Fine-Mist Spray BottleThis economical chromatographic sprayer produces the droplet-free, uniform mist required for both TLC and paper chromatography. Spray bottle includes finger vent for instant spray control.
TLC Plate Desiccator ChamberThis sturdy Plexiglas® desiccator has a foam strip cover lining to prevent moisture and particulate ingress. Accommodates ten 20 x 20 cm plates when used with the TLC plate carrier (34105-55, sold separately at left).
Specifications & Ordering Information
TLC Plate CarrierUse this handy stainless steel rack to transport or store up to ten 20 x 20 cm TLC plates. When carried, handle slightly tilts TLC plates into backstops to ensure safe transport. Fits into TLC plate desiccator (34105-30, sold separately at right).
Specifications & Ordering Information
Specifications & Ordering Information
Specifications & Ordering Information
Wheaton TLC Sample Recovery TubeThis device removes samples from a developed TLC plate by vacuum. Sample is trapped on sealed-in frit and is eluted with solvent for sample analyte recovery.
All wetted parts are made of low extractable borosilicate glass or Teflon® (meets ASTM type 1, Class A, and USP type 1 standards). Recovery tube size is adjustable, thread size is 13-425. Approximate pore size of fritted funnel is 40 to 60 microns.
TLC Plate HolderHold up to 25 TLC plates of any size. Bottom grooves slope toward back vertical support grooves. Plastic construction resists common TLC solvents.
Wheaton TLC Reagent Sprayers and Replacement PartsThese sprayers are ideal for spraying reagents of all types onto both TLC plates and paper chromatographs. Sprayers atomize chromagenic reagents into very fine mists. Sprayers include a convenient finger vent for instant spray control.
Thinline tank with lid 2 KH-34105-15 Standard tank with lid 6* KH-34105-10 Latch-lid tank with lid 6* KH-34105-05 Latch -lid tank with lid and rack 6 KH-34105-40 Aluminum rack 6 KH-34105-50
20 x 20 cmStandard tank with lid 5*
KH-34105-20 Latch-lid tank with lid 5* KH-34105-25 Stainless steel rack 5
Catalog number Description PriceKH-34105-55 TLC plate carrier
Catalog number Dimensions (cm) PriceKH-34105-30 25.4 W x 25.4 H x 24.1 D
Catalog number Description Price
KH-34695-22 Chromatography fine-mist spray bottle
Catalog number Description Size Price
KH-34695-10 Complete sprayer125 mL
KH-34695-12 Head only KH-34695-14 Flask only KH-34695-16 Complete sprayer 250 mL KH-34695-20 Flask only
Catalog number Description PriceKH-34105-60 TLC plate holder
Catalog number Description PriceKH-34695-26 Sample recovery tube
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