SPOT ONThe faster path to MS analysis

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VeriSpray ion source mounted to a Thermo Scientific triple quadrupole mass spectrometer for worry-free operation, faster sample turnaround times (TAT), reduced per sample costs, and high-confidence MS-based results.

SPOT ONThe path to fast MS analysis

Day after day, analytical testing laboratories face

considerable challenges due to backlogs of time-critical

samples, rising per-sample costs, availability of skilled

laboratory technicians, and chromatography

maintenance and downtime.

The Thermo Scientific™ VeriSpray™ PaperSpray ion

source uniquely addresses these concerns. Combine

the VeriSpray ion source with next-generation Thermo

Scientific™ mass spectrometers to reduce time to results

and cost per test with automated, high-throughput,

direct mass spectrometry (MS)-based sample analysis.

The VeriSpray ion source allows use of “dilute and

shoot” methods to minimize solvent consumption

and time-consuming sample preparation steps like

derivatization, while avoiding needs for expert operators

and instrument downtime.

For laboratories currently performing LC or GC methods,

or for those that want to add the robust sensitivity and

selectivity of MS-based tests, the VeriSpray ion source

provides an ideal alternative to the complexity of

chromatography-based solutions.

From sample spotting to MS results—a remarkably simplified workflow. The VeriSpray ion source enables direct MS-based ionization of complex sample matrices spotted on paper—without time-consuming sample preparation and chromatography.

Achieve remarkable simplicity

For many tests, the VeriSpray ion source solution eliminates time-consuming, complicated off-line sample preparation and chromatographic separation, simplifying MS analyses for any user. The sensitivity and selectivity of MS-based analysis and quantification of compounds in complex matrices are now more accessible than ever before.

Reduce sample turnaround time

The VeriSpray ion source solution permits high-throughput analysis of unprocessed samples in just one step. By minimizing sample preparation and eliminating chromatography separation time, MS results can be obtained in less than two minutes. With the VeriSpray ion source, there is no chromatographic downtime or maintenance.

Lower costs per test

Compared to LC, the VeriSpray ion source substantially reduces solvent consumption and disposal, and eliminates LC or GC instrument maintenance costs and extra derivatization steps. By making the specificity and sensitivity of MS results available to non-experts, you can run more tests in house, saving money spent on send outs.

Automate analyses

A VeriSpray ion source solution can run up to 240 samples unattended. For automated sample spotting, the VeriSpray sample plates are compatible with robotic spotters. Barcode reading capabilities enable integration into Laboratory Information Systems (LIS).

Go green

Environmentally friendly, the VeriSpray ion source reduces solvent consumption. The VeriSpray ion source itself consumes very little solvent and produces minimal waste.

Apply Rewet Solvent

Apply Spray Solvent

Apply Spray Voltage to Induce Ionization

3 KV

Concentration ng/mL

Are

a R

atio

Are

a R

atio

ng/mL

2.0

1.6

1.2

0.8

0.4

0.0

0.28

0.24

0.20

0.16

0.12

0.08

0.04

0.00

0 100 200 300 400 500

0 20 40 60 80

EDDP in Whole BloodY = 4.169e-3X – 5.17e-3; R2: 0.9982; Origin: Ignore; W: 1/X; Area

Target level

3

TSQ Quantis VeriSpray TSQ Altis VeriSprayTSQ Quantis VeriSpray TSQ Altis VeriSprayTSQ Quantis VeriSpray TSQ Altis VeriSpray

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STATE-OF-THE-ART DESIGN automates analyses for high throughput and lower costs

With an advanced design, the VeriSpray ion source automates direct MS analyses, helping to improve turnaround time and reduce sample analysis costs.

For high-throughput automation, the VeriSpray sample plates can be spotted using popular robotic spotters. The plate loader holds up to ten plates, allowing unattended analysis of up to 240 samples. Plate loader barcode reading capability streamlines laboratory workflows by allowing integration into LIS. Automation is enhanced with data acquisition by Thermo Scientific™ Xcalibur™ software (version ≥4.3 SP1) and Thermo Scientific™ TraceFinder™ software (version ≥4.1 SP5).

VeriSpray system showing the plate loader with ten-plate magazine, and VeriSpray ion source, installed on a Thermo Scientific triple quadrupole mass spectrometer.

VeriSpray Ion Source

Plate Loader

Plate Magazine

55

VeriSpray 24-sample plate with view of barcode in plate magazine.

VeriSpray 24-sample plate for productivity and tracking

The microplate format of the VeriSpray 24-sample plate fits commercial robotic spotters. The barcode and paper strip provide a unique location identifier for each sample.

VeriSpray ion source operation is fully integrated into the mass spectrometer instrument control software (version ≥3.2).

Single use sample cartridge

The sample plate allows spotting of any fluid directly to the paper cartridge. The sample is applied and allowed to dry, after which it can be analyzed directly with the VeriSpray ion source. Once the sample has been analyzed the cartridge is locked, preventing subsequent re-analysis.

Optional plate loader for walkaway analyses

The plate loader with plate magazine holds up to ten plates of 24-samples each, making it possible to run up to 240 samples unattended.

VeriSpray ion source for simplicity

The VeriSpray ion source transports the sample plate from the plate loader to the inlet of the mass spectrometer for analysis. Spray voltage is applied to the paper to initiate electrospray, and samples may be analyzed in less than two minutes. The VeriSpray ion source can also accommodate manual loading of a single sample plate without use of the plate loader.

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Sample preparation and the complexity of chromatographic separations have posed significant barriers to broad adoption of LC-MS and GC-MS for routine use. The VeriSpray ion source reduces these barriers with direct sample analysis capability that eliminates the need for sample preparation and traditional chromatographic separation for a wide range of applications.

The accuracy, sensitivity, and selectivity of MS are now matched with worry-free operation that reduces sample turnaround time and costs.

Application benefits• Avoids rate-limiting, complex sample preparation

and long chromatographic runs

• Improves sample-to-sample analysis time

• Reduces solvent consumption and waste

WORRY-FREE PRODUCTIVITY for a breadth of applications

Quantitation of cocaine in urine. The chronograms show the presence of cocaine confirmed at 5 ng/mL using the VeriSpray ion source coupled to the TSQ Quantis mass spectrometer. The detection levels shown are more than adequate for most drugs-of-abuse screening applications.

High-confidence quantification of cocaine in urine

5 ng/mLMA: 9.545E5

10 ng/mLMA: 1.440E6

20 ng/mLMA: 2.468E6

50 ng/mLMA: 8.860E6

200 ng/mLMA: 3.022E7

500 ng/mLMA: 9.858E7

1000 ng/mLMA: 2.169E8

2000 ng/mLMA: 5.478E8

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

NL: 3.89E4

NL: 5.10E4

NL: 9.24E4

NL: 3.31E5

NL: 9.83E5

NL: 3.94E6

NL: 5.52E6

NL: 2.17E7

RT :0.00-1.01 TIC MS F: Cocaine:+ c PSI SRM ms2 304.150 [77.124-77.126, 82.195-82.197, 182.195-182.197]

RT :0.00-1.01 TIC MS F: Cocaine:+ c PSI SRM ms2 304.150 [77.124-77.126, 82.195-82.197, 182.195-182.197] 201...

Time

Rel

ativ

e A

bun

dan

ce

Concentration ng/mL

Are

a R

atio

Are

a R

atio

ng/mL

0.8

0.6

0.4

0.2

0.0

0.16

0.12

0.18

0.04

0.00

0 200 400 600 800 1000

0 40 80 120 160 200 220

Target level

Cocaine in UrineY = 7.716e-4X – 4.467e-4; R2: 0.9889; Origin: Ignore; W: 1/X2; Area

Concentration ng/mL

Are

a R

atio

Are

a R

atio

ng/mL

2.0

1.6

1.2

0.8

0.4

0.0

0.28

0.24

0.20

0.16

0.12

0.08

0.04

0.00

0 100 200 300 400 500

0 20 40 60 80

EDDP in Whole BloodY = 4.169e-3X – 5.17e-3; R2: 0.9982; Origin: Ignore; W: 1/X; Area

Target level

7

Overlays of three calibration curves of EDDP in blood, calculated at the beginning, middle, and end of the study, show robust and consistent performance under challenging conditions without sample cleanup. The study involved running 240 samples of EDDP spiked in whole blood with a run time of approximately two minutes that covered an eight-hour shift.

Quantification of antifungal drugs with the VeriSpray ion source coupled to the TSQ Altis mass spectrometer. The VeriSpray ion source makes it possible to obtain these quantification results, quickly without sample send outs. Except for the addition of internal standard solution, no sample preparation was performed prior to analysis. Plasma-based calibration curves for the three antifungal agents showed excellent linearity in the desired quantitative range of 0.1 to 10 µg/mL.

Robust and reproducible Brings more methods in-house, avoiding costly send-outs

Analyte LOD (µg/mL) LOQ (µg/mL)

Voriconazole 0.02 0.06

Itraconazole 0.03 0.09

Posaconazole 0.04 0.15

Number of Matrix Injections

Cal

cula

ted

Co

ncen

trat

ion

(ng

/mL)

100

80

60

40

20

035 85 135 185 235 240

EDDP in Whole BloodQCI = 50 ng/mL

+20% % CV = 1.7%

-20%

Concentration µg/mL

Are

a R

atio

2.8

2.4

2.0

1.6

1.2

0.8

0.4

0.0

0 2 4 6 8 10

PosaconazoleY = 2.791e-1X + 1.701e-4; R2: 0.9981; Origin: Ignore; W: Equal; Area

Range of interest

Spot more at thermofisher.com/VeriSpray

For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. BR65405-EN 0319M

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COMPATIBLE

mass spectrometers

The VeriSpray ion source is compatible with the following Thermo Scientific mass spectrometers.

• Thermo Scientific™ TSQ Altis™ mass spectrometer

• Thermo Scientific™ TSQ Quantis™ mass spectrometer

• Thermo Scientific™ TSQ Fortis™ mass spectrometer

• Thermo Scientific™ TSQ Quantiva™ mass spectrometer

• Thermo Scientific™ TSQ Endura™ mass spectrometer