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UNIVERSITY OF BOLOGNA DiSTA Department of Agroenvironmental Science and Technologies GRiCI – Research Group on Industrial Crops Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 2096652. Fax: 0039 051 2096241. G.Ri.C.I. website: www.agrsci.unibo.it/dista/grici/ BioKenaf Project Contract No: QLK5-CT2002-01729 Second Year Results Second Year Results (WP2 (WP2 - - Task 2.2 _ 2.3) Task 2.2 _ 2.3) Fifth Technical Meeting Fifth Technical Meeting Catania Catania July 5 July 5 - - 6, 2005 6, 2005 Technical staff: Technical staff: Prof Prof G.Venturi G.Venturi Prof M.T. Prof M.T. Amaducci Amaducci Dott Dott . N. Di Virgilio . N. Di Virgilio
Transcript
Page 1: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

UNIVERSITY OF BOLOGNA

DiSTA Department of Agroenvironmental Science and Technologies

GRiCI – Research Group on Industrial Crops –Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 2096652. Fax: 0039 051 2096241. G.Ri.C.I. website: www.agrsci.unibo.it/dista/grici/

BioKenaf Project Contract No: QLK5-CT2002-01729

Second Year ResultsSecond Year Results(WP2 (WP2 -- Task 2.2 _ 2.3)Task 2.2 _ 2.3)

Fifth Technical MeetingFifth Technical MeetingCataniaCatania –– July 5 July 5 -- 6, 20056, 2005

Technical staff: Technical staff: Prof Prof G.VenturiG.Venturi

Prof M.T. Prof M.T. AmaducciAmaducciDottDott. N. Di Virgilio. N. Di Virgilio

Page 2: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

Experimental Farm of Bologna University, Cadriano (Northern Italy)

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

44° 33’ N, 11° 21’ E

altitude: 32 m a.s.l.soil slope: 0% organic matter = 1.9%pH = 7.14

33 m

3 14 4 57

6

3

14

48

35 m

3 7 4 47 7 3

hemp

fieldhemp

field

Task 2.2

Task 2.3

Block 1

Block 2

Block 3

Block 4

Block 4

Block 3

Block 2

Block 1farm soil typology:Udic Ustochreps fine silty, mixed mesic

sand = 19%silt = 53%clay = 28%

"

"

"

"

"

""

"

"

"

"

"

"

"

"" "

" ""ROMA

Bologna Experimental layout

Page 3: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

1 S1 V2 D1 S2 V2 D1 S2 V2 D2 S1 V2 D1 N1 N0

2 S1 V2 D2 S2 V1 D1 S1 V1 D1 S2 V1 D2 N3 N2

3S2 V2 D2 S1 V1 D2 S1 V2 D1 S1 V2 D2 N3 N1

4 S2 V2 D1 S1 V1 D1 S2 V1 D2 S1 V1 D2 N2 N0

5 S2 V1 D2 S1 V2 D2 S2 V1 D1 S2 V2 D1 N0 N1

6 S1 V1 D2 S2 V2 D2 S2 V2 D1 S2 V2 D2 N2 N3

7 S2 V1 D1 S1 V2 D1 S1 V1 D2 S1 V1 D1 N2 N3

8 S1 V1 D1 S2 V1 D2 S1 V2 D2 S2 V1 D1 N0 N1

TASK 2.2 TASK 2.3

Block 4

Block 3

Block 1 Block 2 Block 3 Block 4

Block 2

Block 1

S1S2V1V2D1 20 p m-2

D2 40 p m-2

N0N1N2N3

100 N Kg ha-1

150 N Kg ha-1

Task 2.2

Task 2.30 N Kg ha-1

50 N Kg ha-1

Everglades41Tainung2

1st sowing time (7th May 2004)2nd sowing time (9th June 2004)

First sowing date:7th May 2004Tot. emergence:5 days

Second sowing date:9th June 2004Tot. emergence:6 days Woven non woven tissue

Irrigation after sowingto help emergence

Fertilization:4th June 2004

sowing date:21th May 2004Tot. emergence:5 days

Complete randomized block design for all tested factors

TESTED TREATMENTS AND EXPERIMENTAL LAYOUT OF Task 2.2 and Task 2.3

Page 4: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

COLLECTED DATA

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Repeated harvests during crop cycle Repeated harvests during crop cycle

(rows of 1,5 m)(rows of 1,5 m)• Fresh / dry weight of harvested rowFresh / dry weight of harvested row

•• Number of plantsNumber of plants

•• Height (cm) and Base Stem Diameter (mm)Height (cm) and Base Stem Diameter (mm)

•• Fresh / dry weight of stems, leaves and petiolesFresh / dry weight of stems, leaves and petioles

•• Leaf area (cmLeaf area (cm22))

Meteorological data like Meteorological data like ……•• Daily maximum/minimum air temperature (Daily maximum/minimum air temperature (°°C)C)

•• Rainfall (mm)Rainfall (mm)

•• Air humidity (%)Air humidity (%)

•• Evaporation (glass A panel)Evaporation (glass A panel)

•• Sunshine hoursSunshine hours

•• Water tableWater table

Soil typical data before sowingSoil typical data before sowing

Final harvests of 5 mFinal harvests of 5 m22 (Harvest A and B)(Harvest A and B)•• Biometric parameters Biometric parameters

•• Number of branchesNumber of branches

•• Number of nodesNumber of nodes

•• DecorticationDecortication (fresh / dry weight of bark and core)(fresh / dry weight of bark and core)

•• C and N% in leaves, stems and soil (Harvest A)C and N% in leaves, stems and soil (Harvest A)

•• Ash content (%) in coreAsh content (%) in core

The light response curve of leaf photosynthesis (A)The light response curve of leaf photosynthesis (A)

I II III IV V VI VII VIII IX Xdate 18/6 1/7 15/7 29/7 12/8 2/9 20/9 13/10 17/11 14/12DOY 170 183 197 211 225 246 264 287 322 349DAE 38 51 65 79 93 114 132 155 190 217GDD 258 407 563 744 926 1175 1328 1465 1542 1542DAE 4 17 31 45 59 80 98 121 156 183GDD 35 184 341 521 703 959 1111 1248 1325 1325date 18/6 1/7 15/7 29/7 12/8 3/9 21/9 14/10 18/11 15/12DOY 170 183 197 211 225 247 265 288 323 350DAE 24 37 51 65 79 101 119 142 177 204GDD 190 338 495 676 858 1119 1269 1397 1474 1474

S2

TASK 2.3

Harvest n°

TASK 2.2

S1

Page 5: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

COLLECTED DATA

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

4

Seeds at 10 cm within the row

4

Seeds at 5 cm within the row

Seeds at 5 cm within the row

2

STAR 1 STAR 3STAR 2

• Height (cm) and Base Stem Diameter (mm) for all plantsHeight (cm) and Base Stem Diameter (mm) for all plants

•• Fresh / dry weight Fresh / dry weight

•• Fresh / dry weight of bark at 20, 50, 150 cm from centreFresh / dry weight of bark at 20, 50, 150 cm from centre

•• % of fresh bark% of fresh bark

Effect of the distance between the rows on plant biometric paramEffect of the distance between the rows on plant biometric parameters and yieldseters and yields

Tainung2Sowing: 21st May 2004

Page 6: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

COLLECTED DATA

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Meteorological data

0

5

10

15

20

25

30

35

40

MAY JUN JUL AUG SEP OCT NOV DEC

°C

0

10

20

30

40

50

60

70

80

mmRainfall Rainfall (10 years) T Max

T Min T mean T Max (10 years)

T min (10 years)

Rainfall, Min and Max temperature during the experimental period.

Air temperature followed the typical trend of the local conditions

Rainfalls were almost absent from August until 12th September

50 mm in one event with hail

More rainfall in May and June than 2003

Temperatures during the growing period resulted lower than 2003 mainly during August

Harvest A 195 434,4 1541,8

Harvest B 222 517,4 1541,8

Harvest A 162 385,8 1325,5

Harvest B 189 468,8 1325,5

Harvest A 182 425,2 1473,8

Harvest B 209 508,2 1473,8

TASK 2.2

TASK 2.3

Plant Cycle Lenght (Days)

acc. Rainfalls (mm)

S1

S2

acc. GDD from emerg (°C)

After the first sowing date few days without GDD increasing was observed.

GDD stopped to increase on the beginning of November, few days before harvest A. In 2003 it happens at the middle of October.

Rainfalls from the first sowing (7th May 2004) to the final harvest (15th December 2004) were 517 mm, instead of 380 mm of the last year and of 539 mm as a mean of the last 10 years

Page 7: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Task 2.2 Task 2.3 units method

36 44 %

41 35 %

23 21 %

7,74 7,41 M.III. 1 DM 13-9-99

< 1 < 1 % (Dietrich-Fruehling)

< 1 < 1 % (Drouineau)

9,4 8,6 g kg-1

1,6 1,5 %

1,06 1,09 %o (Dumas)

16 12 meq/100 g (M. 27 DM 11-05-92)

182 169 g kg-1 P2O5 (Olsen)

197 215 g kg-1 K2O (M. 13,5 DM 13-9-99)

2955 2625 g kg-1 CaO (M. 13,5 DM 13-9-99)

308 380 g kg-1 MgO (M. 13,5 DM 13-9-99)

37 33 g kg-1 (M. 13,5 DM 13-9-99)Na exc.Mg exc.

CaCO3 ACT

(Walkley & Black)

Ca exc.

pH (in H20)

Organic Mattertotal N

Organic Carbon

CaCO3 TOT

sandsilt

clay

K exc.

C.E.C.P ass.

Soil analysis results of Task 2.2 and 2.3 fields

Page 8: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

50

60

70

80

90

100%

Tainung2Everglades41

Sowing on 14/04/2004. First germination survey on 19/04/2004. 50 seeds per 4 replications. Incubation room at 20-30 °C.

Second germination survey on 26/4/2004.

Weight 1000 seeds:

Tainung2: 297.1 g

Everglades41: 292.2 g

20% of seed damaged by sowing 20% of seed damaged by sowing machinemachine

Adapted mechanical Adapted mechanical sowing machine sowing machine

““VignoliVignoli””

Seed germination test

5 DAS A B C D mean St. Dev. St. Err

Tainung2 78 90 74 88 82,5 7,72 3,86

Everglades41 88 90 86 98 90,5 5,26 2,63

10 DAS

Tainung2 98 92 100 100 97,5 3,79 1,89

Everglades41 94 96 90 98 94,5 3,42 1,71

96

% of germinated plants after 5 and 10 days of incubation.

Page 9: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Plant germination and emergence on field

D1

D2

Task 2.3

0

5

10

15

20

25

30

35

40

45

50

1/6 1/7 31/7 30/8 29/9 29/10 28/11 28/12

Harvest date

p m -2

D1 was reasonably reached, 18.4 ± 3.5 plants m-2

D2 was 35 ± 5.7 plants m-2

D1 density constant during time

D2 density decreased from 40 p m-2 to 30 p m-2 at final harvests

No important influence of the variety on emergence on field.

Page 10: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Height (cm) and Base stem diameter (mm) during time

S1plant height (cm)

S2

S1

base stem diameter (mm)

S2

0 25 50 75 100 125 150 175 200 225

DAE

cm

0

5

10

15

20

25

30mmplant height (cm)

D1

D2

D1

D2

base stem diameter (mm)

0

25

50

75

100

125

150

175

200

225

150 175 200 225 250 275 300 325 350 375

DOY

cm

0

5

10

15

20

25

30mm

Effect of sowing time Effect of density

V1plant height (cm)

V2

V1

base stem diameter (mm)

V2

150 175 200 225 250 275 300 325 350 375

DOY

cm

0

5

10

15

20

25

30mm

N0

plant height (cm)

base stem diameter (mm)

N2 N1N3

N0N1N2

N3

0

25

50

75

100

125

150

175

200

225

0 25 50 75 100 125 150 175 200 225

DAE

cm

0

5

10

15

20

25

30mm

Effect of genotype Effect of N level

234 cm by S1, D1, Tainung2

164 cm in 2003

D2 density reached the lower heights for both the sowing times.

Task 2.3 similar height for all N levels, N2: 207 cmN0: 190 cm

20 mm Ø by S1 D1 Everglades41

very similar values for all N levels: 16 mm

N0: 14 mm

Last two harvests showed a stabilization of plant height,

the base stem diameter seems to stop increasing already after 75 DAE,

similar to 2003 trends

Page 11: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Fresh yields (t ha-1) of total biomass and stems during time

V1

Fresh biomass

V2

Fresh stems

V1V2

0

10

20

30

40

50

60

70

80

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1

Effect of genotypeVertical lines represent standard error

Fresh biomass

N0

N1

N2Fresh stems

N3

N0N1

N2N3

0

10

20

30

40

50

60

70

80

90

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

Effect of N levelVertical lines represent standard error

Fresh biomassD1

Fresh stems

D1D2

D2

0

10

20

30

40

50

60

70

80

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1Effect of density

Vertical lines represent standard error

S1Fresh biomass

S2

Fresh stems

S1

S2

0

10

20

30

40

50

60

70

80

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

Effect of sowing timeVertical lines represent standard error Best performance for N1

and for S1 Tainung2, D1 and D2

75 t ha-1 Fresh biomass(52 t ha-1 in 2003) at harvest VIII (13th October)

Sowing times affected fresh biomass yield and mainly fresh stem yield,

at the final harvest S1 reached up to 30 t ha-1 of fresh stem instead of 20 of S2.

The stem growth rate for both sowing times was similar until 125 DAE

All trends were also very similar, like 2003 year.

During the last surveys fresh yields decreased significantly, as moisture content on plant decreased too

Page 12: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

S1

S2

0

10

20

30

40

50

60

20 70 120 170

DA E

t ha-1

Fresh stem (t ha-1)of S1 and S2 thesis

S1

S2

0

10

20

30

40

50

60

20 70 120 170

DA E

t ha-1

Fresh stem (t ha-1)of S1 and S2 thesis

Fresh yields (t ha-1) of total biomass and stems

Fresh biomass S1

S2

0

10

20

30

40

50

60

70

80

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

2004 Fresh biomass (t ha-1)

S1

Fresh stems

S2

0

10

20

30

40

50

60

70

80

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

Fresh stem (t ha-1)

S1

S2

0

10

20

30

40

50

60

20 70 120 170

DA E

t ha-1

Fresh biomass (t ha-1)of S1 and S2 thesis

S1

S2

0

10

20

30

40

50

60

20 70 120 170

DA E

t ha-1

Fresh biomass (t ha-1)of S1 and S2 thesis

2003

S1

S2

Page 13: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Stems

S1

S1Petioles

S2

S1

Leaves

S2

S2

0

10

20

30

40

50

60

70

80

90

100

0 25 50 75 100 125 150 175 200 225

DAE

%

petioles

leaves

stems

0

10

20

30

40

50

60

70

80

90

100

0 25 50 75 100 125 150 175 200 225

DAE

%

Effect of sowing time

Fresh biomass partition in Task 2.3

Partition of FB on plant organs during time

The same consideration can be done for N level

Stem % increased during time, Leaves and petioles decreased until to reach almost 0 at the final harvests.

Sowing time seems to have a little effect on partition of biomass in plant organs.

S2 showed higher values of leaves % during time until November.

Density and genotype did not affected the partition of biomass on plant organs.

Page 14: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Dry Biomass yields (t ha-1)

S1

Dry biomass

S2Dry stems

S1

S2

0

5

10

15

20

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1 Effect of sowing time vertical lines represent standard error

Dry biomass

D1Dry stems

D1

D2

D2

0

5

10

15

20

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1 Effect of densityvertical lines represent standard error

V1Dry biomass

V2

Dry stems

V1

V2

0

5

10

15

20

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1Effect of genotype

vertical lines represent standard error

Dry biomass

N0

N1

N2

Dry stems

N3

N0

N1

N2N3

0

5

10

15

20

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

Effect of N level vertical lines represent standard error

Dry biomass increased significantly until October

18 t ha-1 at 155 DAE (11 t ha-1 at 140 DAE in 2003)by S1 D1 Tainung2

Trends of all treatments were very similar.

S1 produced more than S2, as D2 and Tainung2 produced more than D1 and Everglades41

…differences was not so relevant and the growth rates were comparable

At 150 DAE N1 and N3 treatment showed the best performance (up to 18 t ha-1)…high standard error

Page 15: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Dry stem yields (t ha-1)

S1

Dry biomass

S2Dry stems

S1

S2

0

5

10

15

20

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1 Effect of sowing time vertical lines represent standard error

Dry biomass

D1Dry stems

D1

D2

D2

0

5

10

15

20

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1 Effect of densityvertical lines represent standard error

V1Dry biomass

V2

Dry stems

V1

V2

0

5

10

15

20

150 175 200 225 250 275 300 325 350 375

DOY

t ha -1Effect of genotype

vertical lines represent standard error

Dry biomass

N0

N1

N2

Dry stems

N3

N0

N1

N2N3

0

5

10

15

20

0 25 50 75 100 125 150 175 200 225

DAE

t ha -1

Effect of N level vertical lines represent standard error

Trends were quite similar

Best performance was shown by combination S1 D1 Tainung2 (13 t ha-1)

Plants of S1 treatments increased dry stem biomass until around 190 DAE, while S2 (sowed 30 days after S1) stopped to growth around 125 DAE.

This resulted in a quite higher yields of S1 treatments respect to S2

At the last survey in all the cases dry stem yield slightly decreased.

N0 higher values (up to 11 t ha-1)…high standard error

…3 t ha-1 of dry leaves can be estimated for N1 and N3 treatment at harvest VIII

Page 16: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

S1

Dry stems

S2

Dry biomassS1

S2

0

2

4

6

8

10

12

14

16

18

20

0 500 1000 1500 2000

acc GDD

t ha-1

Effect of sowing time on dry biomass yield and stems respect to the accumulation of the GDD (°C)

Dry stem yields (t ha-1)

In all cases S1 is up to S2

S2 needed more growing degree days to reach same values of S1 yield

S1

S2

0

2

4

6

8

10

12

14

20 70 120 170

DAE

t ha-12003

S1

S2

0

2

4

6

8

10

12

14

20 70 120 170 220

DAE

t ha-1 2004The higher value reached by

S1 in 2003 at 170 DAE (5th November) was reached by S1 in 2004 at 120 DAE (8th September),

…even if difference in sowing time was only of 10 days

Page 17: Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 ... · (WP2 - Task 2.2 _ 2.3) Fifth Technical Meeting Catania – July 5 - 6, 2005 Technical staff: Prof G.Venturi ... 5th Technical

RESULTS

BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/

Dry biomass content (%) during time

% dry content in plant

0

10

20

30

40

50

150 175 200 225 250 275 300 325 350 375

DOY

% % dry content in plant

0

10

20

30

40

50

150 175 200 225 250 275 300 325 350 375

DOY

%

Dry biomass content (%) in plant during time in Task 2.2vertical lines represent standard error

Dry biomass content (%) in plant during time in Task 2.3vertical lines represent standard error

Dry matter content increased during time (from 10 to 40 %) a big jump was observed at the final harvest carried out on the middle of December

For all treatments trends were very similar

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RESULTS

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Leaf Area Index and Specific Leaf Area during time

S1

LAI

SLA

S2

S2

S1

0

5

10

15

0 25 50 75 100 125 150 175 200 225

DAE

LAI (m 2 m -2)

0

4

8

12

16

20

24SLA

(m2 kg-1)

Effect of sowing timevertical lines represent standard error

LAI

SLA

D1

D1

D2

D2

0

5

10

15

150 175 200 225 250 275 300 325 350 375

DOY

LAI (m 2 m -2)

0

4

8

12

16

20

24SLA

(m2 kg-1)

Effect of densityvertical lines represent standard error

V1

V2LAI

V2V1 SLA

0

5

10

15

150 175 200 225 250 275 300 325 350 375

DOY

LAI (m 2 m -2)

0

4

8

12

16

20

24SLA

(m2 kg-1)

Effect of genotypevertical lines represent standard error

N0

SLAN0

N1

N1

LAIN2

N2

N3

N3

0

5

10

15

0 25 50 75 100 125 150 175 200 225

DAE

LAI (m 2 m -2)

0

4

8

12

16

20

24SLA

(m2 kg-1)

Effect of N levelvertical lines represent standard error

Sowing time did not affected LAI as final value…even though during the crop cycle S2 trend were usually up to S1 curve

D2 and Tainung2 showed higher values of LAI respect to D1 and Everglades41,… differences were

not relevant

N3 treatment seems to produce more leaves per m-2 during time, but again difference was not so important to consider it.

In terms of SLA, the most relevant difference was showed by D2 density, but in any case trends were very similar.

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RESULTS

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Final harvests (5 m2)

At harvest soil moisture was in a range of 40 % to 50 % (V/V)

Crop did not reached complete flowering

Plants lost leaves before the harvests, all yields are referred to stem

Analysis of variance (ANOVA) for all treatments and their interaction:

Task 2.2Task 2.2

4 factors complete randomized block design

• Variety • Density • Sowing time• Harvest(2 levels)

Task 2.3Task 2.3

2 factors complete randomized block design

• N (4 levels)• Harvest (2 levels)

Differences analyzed at P = 0.05 and 0.01

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Reached density on field was significantly different between the two D thesis.

In both the last harvests n° of plants m-2 was significantly higher for the S1 treatments than S2 ones (P = 0.01)

Trends of some interesting parameters related to the factor that significantly affect their value

0

5

10

15

20

25

30

S1 S2 S1 S2

20 40

Task 2.2

Plants (n° m-2)

Similar to 2003.In plants of S1 and D1 were present more nodes, around 40

Sowing time affected significantly the n° of nodes, S1 10 nodes more than S2.

Everglades41 more nodes than Tainung2 (P=0.05) …little statistical significance also among replications

n° of nodes

05

1015202530354045

S1 S2 S1 S2

Everglades41 Tainung2

Task 2.2

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Height and base stem diameter were influenced mainly by tested densities and sowing times, …as it was observed in 2003.

D1 and S1 treatments reached the higher values.

0

50

150

200

S1 S2 S1 S2

100

20 40

Task 2.2

Plant height (cm)

0

5

10

15

20

S1 S2 S1 S2

20 40

Task 2.2

Base stem diameter (mm)

Trends of some interesting parameters related to the factor that significantly affect their value

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Fresh yields of stems, bark and core were influenced significantly by sowing and harvest time,

The relevant difference between the harvest A and B was mainly due to the lost of moisture, as confirmed by the statistically significant increasing of dry content in stem, bark and core at harvest B respect to harvest A.

Fresh stem yield ranged from up to 55 t ha-1 to 20 t ha-1.

Higher values were observed at harvest A by all first sowing time treatments, 55 against 40 t ha-1 of S2 treatments.

Trends of some interesting parameters related to the factor that significantly affect their value

Fresh stem yieldt/ha

Fresh core yield t/ha

Fresh bark yield t/ha

0

10

20

30

40

50

60

S1 S2 S1 S2

A B

Task 2.2

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Trends of some interesting parameters related to the factor that significantly affect their value

Dry stem yield was influenced mainly by sowing time and density, while harvest time seemed do not affect it.

D1 and S1 treatments reached the highest values, around 13 t ha-1, against 8 t ha-1 of 2003 year.

Also variety and harvest time seems to influence dry stem yield, with a higher production of Tainung2 respect to Everglades41 at harvest A (P=0.05)… little statistical significance also among replications.

Dry bark and core yields are also influenced by sowing time but not by harvest time and density.

Dry core yield ranged from 6.5 to 10 t ha-1, while dry bark reached at maximum 5.8 t ha-1, higher values were reached in all cases again by S1 treatments.

02468

1012

S1 S2 S1 S2

20 40

Task 2.2

Dry stem yield (t ha-1)

0

2

4

6

8

10

12

S1 S2

Task 2.2

Dry bark yield t ha-1

Dry core yield t ha-1

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Trends of some interesting parameters related to the factor that significantly affect their value

A considerable number of plants, from up to 50 % to 95 % of harvested plants, has a branched stem, with at least 2 important ramifications per plant.

Density influenced significantly the presence of the branches, with higher values of branched plants and n° of ramifications per plants reached by D1 density.

S2 plants present more branched stems.

0102030405060708090

20 40 20 40

S1 S2

Task 2.2

Branched plants (% of harvested plants)

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Trends of some interesting parameters related to the factor that significantly affect their value

N2: the highest plant height (P=0.05).

The statistical analysis showed that only the harvest factor seems to affect fresh yields, and dry content

… dry yields were not significantly affected by harvest

180

185

190

195

200

205

N0 N1 N2 N3

Task 2.3

Plant height (cm)

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RESULTSN and C content (%) in leaves, stems and soil at final harvest of Task 2.3

N content in stem and leaf seems to increase with N level until N2

… differences are not relevant, as confirmed by the analysis of the variance

Carbon content in leaf and stems seems also to increase with N level, reaching values up to 43% in leaf of N3 treatment

…differences among treatments are significant at P=0.05

0,0

0,5

1,0

1,5

2,0

2,5

3,0

3,5

leaf stem soil leaf stem soil leaf stem soil leaf stem soil

N0 N1 N2 N3

41,0

41,5

42,0

42,5

43,0

leaf stem soil leaf stem soil leaf stem soil leaf stem soil

N0 N1 N2 N3

Nitrogen content (%)

Carbon content (%)

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RESULTS

QE:QE:apparent quantum efficiencyapparent quantum efficiency

Light compensation pointLight compensation point

Amax:Amax:light saturated maximumlight saturated maximum

K: K: convexityconvexity

Light compensation point = 209

Amax=35.1QE=0.046K=0.605

Saturated PPFD=828

Amax=36.2QE=0.058K=0.274

Light compensation point = 169

Saturated PPFD=784

First First sowingsowing timetime

SecondSecond sowingsowing timetime

S2 higher QE, AmaxS2 higher QE, Amax

S2 lower light S2 lower light compensation pointcompensation point

leaves of S2 more effective leaves of S2 more effective in photosynthesisin photosynthesis

Portable IRGA (CIRASPortable IRGA (CIRAS--2, PP2, PP--Systems)Systems)

Tainung2; D 40 p mTainung2; D 40 p m--22

Most recently fully expanded leavesMost recently fully expanded leaves

The light response curve of leaf photosynthesis (A)

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RESULTS Ash content in core (%)

0,0

1,0

2,0

3,0

4,0

5,0

6,0

7,0

Harvest A Harvest B Harvest A Harvest B

Task 2.2 Task 2.3

Media di Hash (%)

Ash content in Kenaf core is on average around 5.5 %, like more commonly utilized herbaceous plant for energy production

The differences among factors seem to be not important (analysis of the variance)

The harvest time: harvest B showed higher values of ash % for all tested factors, increasing of more than 1 % the hash content, observed only in Task 2.2.

Ash content in core (%)

Harvest A Harvest B Harves t A Harves t B

Everglades41 5,219 6,439 0,708 0,815

Tainung2 4,848 6,121 0,495 0,822

S1 5,176 6,414 0,518 0,874

S2 4,890 6,146 0,712 0,770

D1 5,174 6,156 0,696 0,841

D2 4,893 6,405 0,541 0,809

s tandard deviation

Task 2.2

ash %

N level Harvest A Harvest B Harvest A Harvest BN0 5,574 5,672 0,680 0,061

N1 5,347 6,169 0,366 0,201

N2 5,322 5,913 0,444 0,745

N3 5,637 5,667 0,173 1,063

Task 2.3

ash % standard deviation

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RESULTS

In all the cases fresh weight increased significantly with distance from the star centre.

Also bark yields increased, even if bark % respect to the stem seemed to decrease

Both stem height and base diameter increased going far away from the centre of the star (increasing distance between the row)

Height seems to have a stabilization at the end, where in any case it is possible to observe a big variation.

Lower value of R2 anyway showed a bad estimation of the tendency curve, related to the high variation of the measurement recorded.

A possible reason could be the damages that hail made on plants of this trial, particularly seriously in this trial

Effect of the distance between the rows on plant biometric parameters and yields

height (cm )

base stem diam eter (m m )

R 2 = 0,8465

R 2 = 0 ,7139

0

50

100

150

200

250

0 50 100 150 200 250 300 distance(cm )

(cm

)

0

5

10

15

20

25

30

35

40

45

50

(mm

)

fresh weight (g)

fresh bark (g)

dry bark (g)

% of fresh bark

dry matter in bark (%)

0

200

400

600

800

1000

1200

1400

20 50 150 250 350distance from centre (cm)

(g)

0

5

10

15

20

25

30

35

40

45

50

(%)

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CONCLUSIONS

year 2004 more productively than 2003

Fresh and dry yields, stem height and LAI reached values significantly higher than 2003

2004 2003 2004 2003Height (cm) 234 164 S1 D1 V1 S1 D1 V1

Fresh Yield (t ha -1) 75 52 S1 D1 V1 S1 D2 V1Dry Yield (t ha -1) 18 11 S1 D1 V1 S1 D1 V2

Dry stem yield (t ha -1) 13 9 S1 D1 V1 S1 D1 V1LAI (m 2 m -2) 10 6 S2 D2 V2 S1 D2 V2

Year Year

The higher amount of rainfall, mainly in May and June, respect to 2003

Not particular dry season

Not particular cold temperatures were recorded, at the end of the crop cycle

…plants accumulated more growing degree days respect to the first year

Better climatic conditions:

Max reached values of some interesting parameters in 2004 and 2003

it has to be considered that yield levels of 2003 were very low, as observed for the other crops in the same location

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CONCLUSIONS

Sowing time affected most of investigated parameters, but the difference was lower than what observed during the last year

Increasing crop cycle length…Sowing at the beginning of May increased in 2004 the length of the crop cycle respect to 2003, without any kind of

problems for the plant growth

Two tested varieties did not show important differences as well as nitrogen levels seem not to influence yields, even if in N1 and N3 levels have been observed significantly higher values of LAI

The effects of density were low for the yield parameter, influencing mainly stem diameter and plant height

Harvest time mainly affected the fresh yields, significantly decreasing the moisture content in the last harvest

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KENAF FIBRE

Tainung2

First sowing time

Plant height: 87 cm

Base stem Ø: 11.38 mm

10 X10 XThanks to Federica Pelatti

20 X20 X

10 cm from stem bottom

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SowingSowing

2005 EXPERIMENTAL FIELDS

Task 2.2 S1: 28 04 2005

S2: 30 05 2005

Task 2.3: 10 05 2005

Task 2.2

S2V2 D1

S1V1 D2

S1V2 D2

S2V1 D1

S2V1 D2

S2V2 D2

S1V1 D1

S1V2 D1

S2V2 D2

S2V1 D2

S1V1 D1

S1V1 D2

S1V2 D1

S2V2 D1

S1V2 D2

S2V1 D1

S1V2 D2

S1V2 D1

S2V2 D1

S2V2 D2

S2V1 D1

S1V1 D1

S2V1 D2

S1V1 D2

N1

N3

N0

N2

38 m

4.5 7 4 47

4

7 4.5

48

102 m

Block 1 Block 3

S2V1 D1

S1V2 D1

S1V1 D1

S2V2 D2

S2V2 D1

S1V1 D2

S2V1 D2

N2

N0

N3

N1

N2

N1

N3

N0

N0

N3

N2

N1

7 m

3

6 m

6 m

14 m

24

Block 4

Block 3

Block 2

Block 1

110 m

2 m

24

5 m

S1V2 D2

Block 4

Task 2.2

S2V2 D1

S1V1 D2

S1V2 D2

S2V1 D1

S2V1 D2

S2V2 D2

S1V1 D1

S1V2 D1

S2V2 D2

S2V1 D2

S1V1 D1

S1V1 D2

S1V2 D1

S2V2 D1

S1V2 D2

S2V1 D1

S1V2 D2

S1V2 D1

S2V2 D1

S2V2 D2

S2V1 D1

S1V1 D1

S2V1 D2

S1V1 D2

N1

N3

N0

N2

38 m

4.5 7 4 47

4

7 4.5

48

102 m

Block 1 Block 3

S2V1 D1

S1V2 D1

S1V1 D1

S2V2 D2

S2V2 D1

S1V1 D2

S2V1 D2

N2

N0

N3

N1

N2

N1

N3

N0

N0

N3

N2

N1

7 m

3

6 m

6 m

14 m

24

Block 4

Block 3

Block 2

Block 1

110 m

2 m

24

5 m

S1V2 D2

Block 4

S2V2 D1

S1V1 D2

S1V2 D2

S2V1 D1

S2V1 D2

S2V2 D2

S1V1 D1

S1V2 D1

S2V2 D2

S2V1 D2

S1V1 D1

S1V1 D2

S1V2 D1

S2V2 D1

S1V2 D2

S2V1 D1

S1V2 D2

S1V2 D1

S2V2 D1

S2V2 D2

S2V1 D1

S1V1 D1

S2V1 D2

S1V1 D2

N1

N3

N0

N2

N1

N3

N0

N2

38 m

4.5 7 4 47

4

7 4.5

48

102 m

Block 1 Block 3

S2V1 D1

S1V2 D1

S1V1 D1

S2V2 D2

S2V2 D1

S1V1 D2

S2V1 D2

N2

N0

N3

N1

N2

N0

N3

N1

N2

N1

N3

N0

N2

N1

N3

N0

N0

N3

N2

N1

N0

N3

N2

N1

7 m

3

6 m

6 m

14 m

24

Block 4

Block 3

Block 2

Block 1

110 m

2 m

24

5 m

S1V2 D2

Block 4

HarvestsHarvests21 06 2005

28 06 2005


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