How to print organ?How to print organ?

Vladimir Mironov MD, PhDVladimir Mironov MD, PhDDirector of Director of

MUSC MUSC Bioprinting Bioprinting CenterCenterMedical University of South CarolinaMedical University of South Carolina

Charleston, South Carolina, USACharleston, South Carolina, USA

Kidney price: $30,Kidney price: $30,000.00.00

Tissue assembly technologiesTissue assembly technologies

1. Rolling1. Rolling

2. Embedding2. Embedding

3. Seeding3. Seeding

How to print organ?How to print organ?

Part I.Part I.EngineeringEngineeringaspectsaspects

What is organ printing?What is organ printing?

Automatic computerAutomatic computer--aided aided layer by layer depositionlayer by layer deposition of of living and biologically relevant living and biologically relevant materials with purpose of rapid materials with purpose of rapid manufacturing of functional manufacturing of functional human organhuman organ

ComputerComputer--assisted assisted prototype polymer scaffold printingprototype polymer scaffold printing

ThericsTherics Inc, NJ, USA Inc, NJ, USA Rapid prototyping technologyRapid prototyping technology

Organ printing technologyOrgan printing technology

nScriptnScript Inc.(SciperioInc.(Sciperio), USA ), USA Organ printing technologyOrgan printing technology

Three Main Steps in Three Main Steps in Organ Printing TechnologyOrgan Printing Technology

1. Preprocessing1. Preprocessing(CAD, blueprints, preconditioning)(CAD, blueprints, preconditioning)2. Processing2. Processing(actual printing, solidification)(actual printing, solidification)3. 3. PostprocessingPostprocessing(perfusion, (perfusion, postconditioningpostconditioning,,accelerated tissue maturation)accelerated tissue maturation)

How to print organ?How to print organ?

1. Preprocessing1. Preprocessing(CAD, blueprints, preconditioning)(CAD, blueprints, preconditioning)

Blueprint for HeartBlueprint for Heart

Blueprints for VasculatureBlueprints for Vasculature

Blueprint for VasculatureBlueprint for Vasculature

According to TissueInformatics, Inc.

Blueprint for VasculatureBlueprint for Vasculature

Blueprint for VasculatureBlueprint for Vasculature(from R. Karch et al., 1999, 2000)

How to print organ?How to print organ?

2. Processing2. Processing(actual printing, solidification)(actual printing, solidification)

BioprinterBioprinter is probably not a big problem ...is probably not a big problem ...

ThericsTherics EnvisiontechEnvisiontechSciperioSciperio

SandiaSandia NLNL

Canon, Epson,Canon, Epson,HP, LexmarkHP, LexmarkNeatcoNeatco

NeatcoNeatco Inc. CanadaInc. Canada

Methods of DispensingMethods of Dispensing

Modeling of Printed Vascular UnitModeling of Printed Vascular Unit

V

A

Sometimes modeling Sometimes modeling is not a bad idea...is not a bad idea...

Roadmap for organ printingRoadmap for organ printing

How to print organ?How to print organ?

3. Postprocessing3. Postprocessing(perfusion, accelerated tissue maturation, (perfusion, accelerated tissue maturation, postconditioningpostconditioning))

Perfusion bioreactorPerfusion bioreactor

……one of “Six technologies that will change the world”one of “Six technologies that will change the world”

…at least according to magazine “Business -2.0”

Organ Printing TechnologyOrgan Printing Technology

Printing of living organs Printing of living organs will be another pioneering will be another pioneering

step in the history of science, step in the history of science, technology and civilizationtechnology and civilization

BioprintingBioprinting in 2024in 2024

Here is what Michael Dell (Dell Inc) saidin his speech at Hitotsubashi University, Japan (24 May 2004):(Micheal Dell started his company when he was 19 years old student with only $1,000 cash. 20 years later Dell Inc is one of fastest growing $50bln company with $14bln cash)

Question:"How could you imagine yourself, your company in 2024?"

Michael Dell’s answer:”… I think certainly by the time there will be a whole new industryas you combine the physical sciences and the biological sciences andwe don't quite know what that will look like, but I think it's an incredible opportunity to change medicine and health.That brings me back to what I started to study in college, to be a doctor”.