Contents of Volume 10

Number 1, January 1991Mini-reviewsInteractions between herpesviruses and retroviruses : implications

in the initiation of disease . J . F. Evermann, D . Derse and P . L Dorn1

Progress in the pathogenesis of Legionella pneumophila .N. C. Engleberg and B . I . Eisenstein 11

ArticlesCharacterization of the Helicobacterpylori urease and purification

of its subunits . D. J . Evans, Jr, D. G . Evans, S . S . Kirkpatrick and D . Y. Graham

15

The effect of site and mode of expression of a heterologousantigenic determinant on the properties of poliovirus hybrids .A. D . Murdin, C . Mirzayan, A . Kameda and E . Wimmer27

Phenotypic characterization of antigenic hybrids of poliovirus .A. D. Murdin, A. Kameda, M . G . Murray and E . Wimmer39

Expression cloning of Yersinia enterocolitica 0 :3 rfb gene cluster inEscherichia coli K12 . A. Al-Hendy, P . Toivanen and M . Skurnik47

Similarity of the tdh gene-bearing plasmids of Vibrio cholerae non-01 and Vibrio parahaemolyticus. K. Baba, H . Shirai, A . Terai, K . Kumagai,Y. Takeda and M . Nishibuchi 61

Characterization of classic and atypical serogroup 0 :11 Aeromonas :evidence that the surface array protein is not directly involved inmouse pathogenicity . R. P. Kokka, N . A. Vedros and J . M . Janda71

Short communicationThe effect of growth temperature on the biosynthesis of Yersinia

enterocolitica 0:3 lipopolysaccharide: temperature regulates thetranscription of therfb but not of the rfaregion .A. Al-Hendy, P . Toivanenand M . Skurnik 81

Number 2, February 1991Mini-reviewsThe low-Ca 2+ response virulence regulon of human-pathogenic

yersiniae . S.C. Straley 87

ArticlesAnalysis of LPS released from Salmonella abortus equi in human

serum . M . A. Freudenberg, U . Meier-Dieter, T . Staehelin and C . Galanos93

Detection of toxic viral-associated double-stranded RNA(dsRNA)in influenza-infected lung . J . A. Majde, R . K. Brown, M . W. Jones, C. W .Dieffenbach, N . Maitra, J . M . Krueger, A . B. Cady, C . W. Smitka and H . F .Maassab 105

Comparison of the human immune response to live oral, killed oral orkilled parenteral Salmonella typhi TY21A vaccines . A. Kantele, H .Arvilommi, J . M . Kantele, L . Rintala and P . H . Makela117

Virulence determinants of Escherichia coli 06 extraintestinalisolates analysed by Southern hybridizations and DNA long rangemapping techniques . G . Blum, M . Ott, A. Cross and J . Hacker127

Characterization of Borrelia burgdorferi invasion of culturedendothelial cells . L . E. Comstock and D . D . Thomas137

Immunity conferred by Aro - salmonella live vaccines . C. E .Hormaeche, H . S . Joysey, L . Desilva, M . Izhar and B . A . D . Stocker149

Short communicationsImmunogenicity and protective efficacy of pertussis toxin subunit

S1 produced by Bacillus subtilis. R .-M . Olander, A. Muotiala, J .-P .Himanen, M . Karvonen, U . Airaksinen and K . Runeberg-Nyman159

Characterization of thermostable direct haemolysins encoded byfour representative tdh genes of Vibrio parahaemolyticus . M . Yoh,T. Honda, T. Miwatani and M . Nishibuchi 165

Number 3, March 1991

ArticlesExtracellular lipase of Pseudomonas aeruginosa : biochemical

characterization and effect on human neutrophil and monocytefunction in vitro. K.-E . Jaeger, A . Kharazmi and N . HOiby173

Plasmid-associated virulence factors of non-toxigenic (pXO1 - )Bacillus anthracis. S . L . Welkos 183

Guinea-pig alveolar macrophages kill Mycobacterium tuberculosisin vitro, but killing is independent of susceptibility to hydrogenperoxide or triggering of the respiratory burst . S . O'Brien, P . S . Jackett,D. B. Lowrie and P . W. Andrew 199

Identification of Streptococcus pyogenes proteins that bind torabbit kidney in vitro and in vivo . I . Glurich, B . Winters, B . Albini andM . Stinson 209

Comparison of host responses after intranasal infection of guinea-pigs with Mycoplasma genitalium or with Mycoplasma pneumoniae .E . Jacobs, T . Watter, H . E . Schaefer and W. Bredt 221

Interactions of Haemophilus influenzae with cultured humanendothelial cells . M . Virji, H . Kayhty, D . J . P . Ferguson, C . Alexandrescu andE . R. Moxon 231

Short communicationStrain differences in expression of virulence by the 90 kilobase pair

virulence plasmid of Salmonella serovar Typhimurium . J . T. Ou andL. S. Baron 247

Number 4, April 1991

Mini-reviewMechanisms of herpesvirus immuno-evasion . D. R . Fitzpatrick and

H . Bielefeldt-Ohmann 253

ArticlesComplement-mediated serum activities against genetically defined

capsular transformants of Haemophilus influenzae . A . J . Swift,E. R. Moxon, A. Zwahlen and J . A . Winkelstein 261

Serological conservation and location of the adhesin of avianEscherichia coli type 1 fimbriae . N . K. Chanteloup, M . Dho-Moulin,E. Esnault, A . Bree and J .-P . Lafont 271

Characterization of a non-hemolytic mutant of Actinobacilluspleuropneumoniae serotype 5: role of the 110 kilodalton hemolysin invirulence and immunoprotection . T . J . Inzana, J . Todd, J . Ma and H . Veit 281

A 'safe-site' for Salmonella typhimurium is within splenic cellsduring the early phase of infection in mice . N . E . Dunlap,W. H . Benjamin, Jr, R . D . McCall, Jr, A . B . Tilden and D . E . Briles297

Characterization of outer membrane and secreted proteins ofLeptospira interrogansserovar pomona . R . L . Zuerner, W . Knudtson, C . A .Bolin and G . Trueba 311

Neisseria gonorrhoeae LPS variation, serum resistance and itsinduction by cytidine 5'-monophospho-N-acetylneuraminic acid .R . Demarco de Hormaeche, R . van Crevel and C . E. Hormaeche323

Number 5, May 1991

ArticlesLysophospholipase activity of Ascaris suum-induced mouse

peritoneal neutrophils and eosinophils . H . E. Laubach and P. A . Hall333

Kinetics of intraepithelium and lamina propria lymphocyteresponses during Giardia lamblia infection in mice . V. K. Vinayak,R. Khanna and K . Kum 343

Genetic characterization of the 04 polysaccharide gene clusterfrom Escherichia coli . G . E . Haraguchi, U. Zahringer, B . Jann, K . Jann, R . A .Hull and S . I . Hull 351

Effects of vaccinia virus-expressed interleukin 2 on the immunesystem of sublethally irradiated mice . M . E. Andrew, G . Karupiah,D . B . Boyle, R . V . Blanden, A . Mullbacher, I . A. Ramshaw and B. E . H . Coupar 363

Effect of human chorionic gonadotropin (hCG) on Neisseriagonorrhoeae invasion of and IgA secretion by human fallopian tubemucosa . G . L . Gorby, C. M. Clemens, L . R . Barley and Z . A. McGee373

rIL-1a enhances adoptive transfer of resistance to Listeriamonocytogenes infection . M . Haak-Frendscho, R . S . Kurtz andC. J. Czuprynski 385

Expression of Bordetellapertussisfimbrial (fim) genes in Bordetellabronchiseptica : fimX is expressed at a low level and vir-regulated .B . Riboli, P . Pedroni, A . Cuzzoni, G . Grandi and F . de Ferra393

Short communicationsAbsence of extracellular enzyme activity and cytotoxicity in cell-

free culture filtrates of Haemophilus ducreyi . D. Abeck, A . P . Johnson,A. Hartinger, M . Kollmann, H . C . Korting, R . C . Ballard and O. Braun-Falco405

Neutralizing monoclonal antibodies to Pasteurella haemolyticaleukotoxin affinity-purify the toxin from crude culture supernatants .M . J . Gentry and S . Srikumaran 411

Number 6, June 1991Articles

Antibodies may act synergistically or additively with interferon toinhibit poliovirus . M. P. Langford, R. Crainic, R . Vrijsen and E. Wimmer419

Escherichia coli CS31A fimbriae: molecular cloning, expression andhomology with the K88 determinant . C. Martin, C. Boeuf and F. Bousquet 429

Development and design of a novel in vivo chamber implant for theanalysis of microbial virulence and assessment of antimicrobialtherapy. W. J . Pike, A . Cockayne, C . A . Webster, R . C . B . Slack, A . P . Sheltonand J . P. Abuthnott 443

Chemotaxis of polymorphonuclear leukocytes to varicella-zostervirus antigens . T. Ihara, N . Yasuda, H . Kamiya, S . Torigoe and M . Sakurai . . . . 451

Positive regulator for the expression of Mba protein of the virulenceplasmid, pKDSC50, of Salmonella choleraesuis . H . Matsui, A . Abe,K . Kawahara, N. Terakado and H . Danbara 459

The characterization of lectin-binding proteins of Chlamydiatrachomatis as glycoproteins . A. F . Swanson and C . -c . Kuo465

Short communicationsAdhesin gene of Mycoplasma genitalium exists as multiple copies .

S . F . Dallo and J . B. Baseman 475

Production of type 1 fimbriae by Escherichia coli HB101 . S . J . Elliott,N . Nandapalan and B . J. Chang 481

The frequency of fim genes among Salmonella serovars.D. L. Swenson, S. Clegg and D . C . Old 487

Tn5 mutagenesis of the Salmonella typhimurium 100 kb plasmid :definition of new virulence regions . D . R . Sizemore, P. S . Fink, J . T . Ou,L. Baron, D . J . Kopecko and R . L . Warren 493

Volume contents and index to volume 10 i

Index to Volume 10Author Index

Abe, A . See Matsui, H . 459Abeck, D., Johnson, A . P ., Hartinger, A ., Kollmann, M ., Korting, H . C ., Ballard, R . C . and Braun-Falco, 0 . Absence

of extracellular enzyme activity and cytotoxicity in cell-free culture filtrates of Haemophilus ducreyi 405Airaksinen, U . See Olander, R.-M . 159Albini, B. See Glurich, I . 209Alexandrescu, C. See Virji, M . 231Al-Hendy, A., Toivanen, P . and Skurnik, M . Expression cloning of Yersinia enterocolitica 0:3 rfb gene cluster in

Escherichia coli K12 47Al-Hendy, A ., Toivanen, P . and Skurnik, M . The effect of growth temperature on the biosynthesis of Yersinia

enterocolitica 0:3 lipopolysaccharide : temperature regulates the transcription of the rfb region 81Andrew, M . E ., Karupiah, G ., Boyle, D . B ., Blanden, R . V ., Mullbacher, A ., Ramshaw, I . A . and Coupar, B . E . H . Effects

of vaccinia virus-expressed interleukin 2 on the immune system of sublethally irradiated mice 363Andrew, P . W . See O'Brien, S . 199Arbuthnott, J . P . See Pike, W . J . 443Baba, K ., Shirai, H ., Terai, A ., Kumagai, K .,Takeda, Y . and Nishibuchi, M . Similarity ofthetdhgene- bearing plasmids

of Vibrio cholerae non-01 and Vibrio parahaemolyticus 61Ballard, R . C . See Abeck, D . 405Barley, L . R . See Gorby, G . L . 373Baron, L . See Sizemore, D . R . 493Baron, L . S . See Ou, J . T . 247Baseman, J . B . See Dallo, S . F . 475Benjamin, Jr, W . H . See Dunlap, N . E . 297Bielefeldt-Ohmann, H . See Fitzpatrick, D . R . 253Blanden, R . V. See Andrew, M . E . 363Blum, G., Ott, M ., Cross, A . and Hacker, J . Virulence determinants of Escherichia coli 06 extraintestinal isolates

analysed by Southern hybridizations and DNA long range mapping techniques 127Boeuf, C. See Martin, C . 429Bolin, C . A . See Zuerner, R . L . 311Bousquet, F . See Martin, C . 429Boyle, D . B . See Andrew, M . E. 363Braun- Falco, 0. See Abeck, D . 405Bree, A. See Chanteloup, N . K. 271Bredt, W . See Jacobs, E . 221Briles, D . E . See Dunlap, N . E. 297Brown, R . K . See Majde, J . A . 105Cady, A . B . See Majde, J . A . 105Chang, B . J . See Elliott, S . J . 481Chanteloup, N . K ., Dho-Moulin, M ., Esnault, E ., Bree, A . and Lafont, J.-P . Serological conservation and location

of the adhesin of avian Escherichia coli type 1 fimbrriae 271Clegg, S. See Swenson, D . L . 487Clemens, C . M . See Gorby, G . L. 373Cockayne, A . See Pike, W . J . 443Comstock, L . E . and Thomas, D . D . Characterization of Borrelia burgdorferi invasion of cultured endothelial

cells 137Coupar, B . E . H . See Andrew, M . E . 363Crainic, R . See Langford, M . P . 419Cross, A . See Blum, G .

127Cuzzoni, A . See Riboli, B . 393Czuprynski, C . J . See Haak-Frendscho, M . 385Dallo, S . F . and Baseman, J . B . Adhesin gene of Mycoplasma genitalium exists as multiple copies 475Danbara, H . See Matsui, H . 459de Ferra, F . See Riboli, B . 393Demarco de Hormaeche, R ., van Crevel, R . and Hormaeche, C . E . Neisseria gonorrhoeae LPS variation, serum

resistance and its induction by cytidine 5'-monophospho-N-acetylneuraminic acid 323Derse, D . See Evermann, J . F . 1Desilva, L. See Hormaeche, C . E. 149Dho-Moulin, M . See Chanteloup, N . K. 271Dieffenbach, C . W . See Majde, J . A . 105Dorn, P . L . See Evermann, J . F . 1Dunlap, N . E ., Benjamin, W. H ., Jr, McCall, R . D ., Jr, Tilden, A . B . and Briles, D . E . A 'safe-site' for Salmonella

typhimurium is within splenic cells during the early phase of infection in mice 297

viii

Author Index

Eisenstein, B . I . See Engleberg, N . C .

1 1Elliott, S . J ., Nandapalan, N . and Chang, B . J . Production of type 1 fimbriae by Escherichia co/i HB101

481Engleberg, N . C . and Eisenstein, B . I . Progress in the pathogenesis of Legionella pneumophila 11Esnault, E . See Chanteloup, N . K . 271Evans, D . G . See Evans, D . J ., Jr, 15Evans, D . J ., Jr, Evans, D . G ., Kirkpatrick, S . S . and Graham, D . Y . Characterization of the Helicobacterpylori urease

and purification of its subunits 15Evermann, J . F ., Derse, D, and Dorn, P . L . Interactions between herpesviruses and retroviruses : implications in the

initiation of disease 1Ferguson, D . J . P . See Virji, M . 231Fink, P . S . See Sizemore, D . R . 493Fitzpatrick, D . R . and Bielefeldt-Ohmann, H. Mechanisms of herpesvirus immuno-evasion 253Freundenberg, M . A ., Meier-Dieter, U ., Staehelin, T . and Galanos, C . Analysis of LPS released from Salmonella

abortus equi in human serum 93Galanos, C. See Freundenberg, M. A . 93Gentry, M. J . and Srikumaran, S . Neutralizing monoclonal antibodies to Pasteurella haemolytica leukotoxin

affinity-purify the toxin from crude culture supernatants 411Glurich, I ., Winters, B ., Albini, B . and Stinson, M . Identification of Streptococcus pyogenes proteins that bind to

rabbit kidney in vitro and in vivo 209Gorby, G . L ., Clemens, C . M ., Barley, L . R . and McGee, Z . A . Effect of human chorionic gonadotrophin (hCG) on

Neisseria gonorrhoeae invasion of and IgA secretion by human fallopian tube mucosa 373Graham, D . Y . See Evans, D . J ., Jr, 15Grandi, G. See Riboli, B . 393Haak-Frendscho, M ., Kurtz, R . S . and Czuprynski, C . J . rI L-1x enhances adoptive transfer of resistance to Listeria

monocytogenes infection 385Hacker, J . See Blum, G . 127Hall, P . A . See Laubach, H . E . 333Haraguchi, G . E ., Zahringer, U ., Jann, B ., Jann, K ., Hull, R . A . and Hull, S . I . Genetic characterization of the 04

polysaccharide gene cluster from Escherichia co/i 351Hartinger, A . See Abeck, D . 405Himanen, J .-P . See Olander, R.-M . 159H¢iby, N. See Jaeger, K .-E . 173Honda, T. See Yoh, M . 165Hormaeche, C . E ., Joysey, H . S ., Desilva, L ., Izhar, M . and Stocker, B . A. D. Immunity conferred by Aro salmonella

live vaccines 149Hormaeche, C . E . See Demarco de Hormaeche, R . 323Hull, R . A . See Haraguchi, G . E . 351Hull, S . I . See Haraguchi, G . E . 351Ihara, T ., Yasuda, N ., Kamiya, H ., Torigoe, S . and Sakurai, M . Chemotaxis of polymorphonuclear leukocytes to

varicella-zoster virus antigens 451Inzana, T . J ., Todd, J ., Ma, J . and Veit, H . Characterization of a non-hemolytic mutant of Actinobacillus

pleuropeneumoniae serotype 5 : role of the 110 kilodalton hemolysin in virulence and immunoprotection 281Izhar, M . See Hormaeche, C . E . 149Jackett, P . S . See O'Brien, S . 199Jacobs, E ., Watter, T., Schaefer, H . E . and Bredt, W . Comparison of host responses after intranasal infection of

guinea-pigs with Mycoplasma genitalium or with Mycoplasma pneumoniae 221Jaeger, K.-E ., Kharazmi, A . and H$iby, N . Extracellular lipase of Pseudomonas aeruginosa : biochemical

characterization and effect on human neutrophil and monocyte function in vitro 173Janda, J . M . See Kokka, R . P . 71Jann, B . See Haraguchi, G . E . 351Jann, K . See Haraguchi, G . E . 351Johnson, A . P . See Abeck, D . 405Jones, J . W . See Majde, J . A. 105Joysey, H . S . See Hormaeche, C . E . 149Kameda, A . See Murdin, A . D . 27,39Kamiya, H. See Ihara, T. 451Kantele, J . M ., Rintala, L . and Makela, P . H . Comparison of the human immune response to live oral, killed oral or

killed parenteral Salmonella typhi TY21 A vaccines 117Karupiah, G. See Andrew, M . E . 363Karvonen, M . See Olander, R.-M . 159Kawahara, K . See Matsui, H . 459Kayhty, H . See Virji, M . 231Khanna, R. See Vinayak, V . K . 343Kharazmi, A . See Jaeger, K .-E . 173Kirkpatrick, S . S . See Evans, D . J ., Jr 15Knudtson, W. See Zuerner, R . L . 311Kokka, R . P ., Vedros, N . A. and Janda, J . M . Characterization of classic and atypical serogroup 0 : 11 Aeromonas :

evidence that the surface array protein is not directly involved in mouse pathogenicity 71

Author Index

Ix

Kollman, M . See Abeck, D . 405Kopecko, D . J . See Sizemore, D . R . 493Korting, H . C . See Abeck, D . 405Krueger, J . M . See Majde, J . A . 105Kum, K. See Vinayak, V . K . 343Kumagai, K . See Baba, K . 61Kuo, C.-C . See Swanson, A . F . 465Kurtz, R . S . See Haak-Frendscho, M . 385Lafont, J.-P . See Chanteloup, N . K . 271Langford, M . P ., Crainic, R ., Vrijsen, R . and Wimmer, E. Antibodies may act synergistically or additively with

interferon to inhibit poliovirus 419Laubach, H . E . and Hall, P . A . Lysophospholipase activity of Ascaris suum-induced mouse peritoneal neutrophils

and eosinopohils 333Lowrie, D . B . See O'Brien, S . 199Ma, J . See Inzana, T . J . 281Maassab, H . F . See Majde, J . A . 105McCall, R . D ., Jr See Dunlap, N . E . 297McGee, Z. A . See Gorby, G . L . 373Maitra, N . See Majde, J . A . 105Majde, J . A ., Brown, R . K ., Jones, M . W., Dieffenbach, C . W ., Maitra, N ., Krueger, J . M ., Cady, A . B ., Smitka, C . W .

and Maassab, H . F . Detection of toxic viral-associated double-stranded RNA(dsRNA) in influenza-infectedlung 105

Makela, P . H . See Kantele, J . M . 117Martin, C ., Boeuf, C . and Bousquet, F . Escherichia co/i CS31 A fimbriae : molecular cloning, expression and

homology with the K88 determinant 429Matsui, H ., Abe, A ., Kawahara, K ., Terakado, N . and Danbara, H . Positive regulatorforthe expression of Mba protein

of the virulence plasmid, pKDSC50, of Salmonella choleraesuis 459Meier-Dieter, U . See Freundenberg, M . A . 93Mirzayan, C. See Murdin, A . D . 27Miwatani, T. See Yoh, M . 165Moxon, E . R . See Swift, A . J . 261Moxon, E . R . See Virji, M . 231Mullbacher, A. See Andrew, M . E . 363Muotiala, A. See Olander, R .-M . 159Murdin, A . D ., Kameda, A ., Murray, M . G . and Wimmer, E . Phenotypic characterization of antigenic hybrids of

poliovirus 39Murdin, A . D ., M irzayan, C ., Kameda, A . and Wimmer, E . The effect of site and mode of expression of a heterologous

antigenic determinant on the properties of poliovirus hybrids 27Murray, M. G . See Murdin, A . D . 39Nandapalan, N. See Elliott, S . J . 481Nishibuchi, M . See Baba, K . 61Nishibushi, M . See Yoh, M . 165O'Brien, S ., Jackett, P . S ., Lowrie, D . B . and Andrew, P . W . Guinea-pig alveolar macrophages kill Mycobacterium

tuberculosis in vitro, but killing is independent of susceptibility to hydrogen peroxide or triggering of therespiratory burst 199

Olander, R.-M ., Muotiala, A ., Himanen,J .-P ., Karvonen, M ., Airaksinen, U . and Runeberg-Nyman, K . Immunogenicityand protective efficacy of pertussis toxin subunit S1 produced by Bacillus subtilis 159

Old, D . C . See Swenson, D . L . 487Ott, M . See Blum, G . 127Ou, J . T . and Baron, L . S . Strain differences in expression of virulence by the 90 kilobase pair virulence plasmid of

Salmonella serovar Typhimurium 247Ou, J . T . See Sizemore, D . R . 493Pedroni, P . See Riboli, B . 393Pike, W . J ., Cockayne, A ., Webster, C . A ., Slack, R . C . B ., Shelton, A . P. and Arbuthnott, J . P . Development and

design of a novel in vivo chamber implant for the analaysis of microbial virulence and assessment ofantimicrobial therapy 443

Ramshaw, I . A . See Andrew, M . E . 363Riboli, B ., Pedroni, P ., Cuzzoni, A., Grandi, G . and de Ferra, F . Expression ofBordetellapertussisfimbrial (fim) genes

in Bordetella bronchiseptica : fimX is expressed at a low level and vir-regulated 393Rintala, L . See Kantele, J . M . 117Runeberg-Nyman, K. See Olander, R .-M . 159Sakurai, M . See Ihara, T . 451Schaefer, H . E . See Jacobs, E . 221Shelton, A . P . See Pike, W . J . 443Shirai, H. See Baba, K . 61Sizemore, D . R ., Fink, P . S ., Ou, J . T ., Baron, L ., Kopecko, D . J . and Warren, R . L . Tn5 mutagenesis of the Salmonella

typhimurium 100 kb plasmid : definition of new virulence regions 493

Straley, S . C . The low-Ca" response virulence regulon of human-pathogenic yersiniae 87Swanson, A . F . and Kuo, C.-c . the characterization of lectin-binding proteins of Chlamydia trachomatis as

glycoproteins 465Swenson, D . L ., Clegg, S . and Old, D . C . The frequency of fico genes among Salmonella serovars 487Swift, A . J ., Moxon, E . R ., Zwahlen, A . and Winkelstein, J . A . Complement-mediated serum activities against

genetically defined capsular transformants of Haemophilus influenzae 261Takeda, Y . See Baba, K . 61Terai, A. See Baba, K . 61Terakado, N . See Matsui, H . 459Thomas, D . D . See Comstock, L . E . 137Tilden, A . B . See Dunlap, N . E . 297Todd, J . See Inzana, T . J . 281Toivanen, P . See Al-Hendy, A . 47,81Torigoe, S . See lhara, T . 451Trueba, G. See Zuerner, R . L . 311van Crevel, R . See Demarco de Hormaeche, R . 323Vedros, N . A . See Kokka, R . P . 71Veit, H . See Inzana, T . J . 281Vinayak, V. K ., Khanna, R . and Kum, K. Kinetics of intraepithelium and lamina propria lymphocyte responses during

Giardia lamblia infection in mice 343Virji, M ., Kayhty, H ., Ferguson, D . J . P ., Alexandrescu, C. and Moxon, E . R . Interactions of Haemophilusinfluenzae

with cultured human endothelial cells 231Vrijsen, R. See Langford, M . P . 419Warren, R . L . See Sizemore, D . R . 493Watter, T. See Jacobs, E . 221Webster, C . A . See Pike, W . J . 443Wekkos, S . L . Plasmid-associated virulence factors of non-toxigenic (pXO1 ) Bacillus anthracis 183Wimmer, E. See Langford, M . P . 419Wimmer, E. See Murdin, A . D . 27, 39Winkelstein, J . A . See Swift, A . J . 261Winters, B . See Glurich, I . 209Yasuda, N . See lhara, T . 451Yoh, M ., Honda, T ., Miwatani, T . and Nishibuchi, M . Characterization of thermostable direct haemolysins encoded

by four representative tdh genes of Vibrio parahaemolyticus 165Zahringer, U . See Haraguchi, G . E . 351Zuerner, R . L ., Knudtson, W ., Bolin, C . A . and Trueba, G . Characterization of outer membrane and secreted proteins

of Leptospira interrogans serovarpomona 311Zwahlen, A . See Swift, A . J. 261

x Author Index

Skurnik, M . See Al-Hendy, A . 47,81Slack, R . C . B . See Pike, W . J . 443Smitka, C . W . See Majde, J . A . 105Srikumaran, S . See Gentry, M . J . 411Staehelin, T. See Freundenberg, M . A . 93Stinson, M . See Glurich, I . 209Stocker, B . A . D . See Hormaeche, C . E . 149

Fimbriae, type 1 271,481Frequency 487Gene probes 127Gentamicin 297Giardia lamblia 343Glycoproteins 451, 465Gonococci 323, 373Growth characteristic 27Growth rate 39Haemagglutination 481Haemophilus ducreyi 405

Mycobacterium tuberculosis 199Mycoplasma genitalium 221, 475Mycoplasma pneumoniae 221Neisseria gonorrhoeae 323, 373Nematoda 333Nephritis 209Neutralization 419Neutrophil 173,333O-antigen biosynthesis 351OmpA-LPS complex 93Opsonic activity 261

Subject Index

Actinobacillus pleuropneumoniaeAdhesin 271, 429, 475Adhesion 481

281 Haemophilus influenzaeHelicobacter pylori 15Helper/inducer T cells

231,

343

261

25393

Adoptive transfer 385Aeromonas 71

Hemolysin 165, 281Herpesvirus 1

Affinity-purification 411Antibody 419

Herpesvirus immuno-evasionHigh density lipoprotein-LPS-complex

Antibody-secreting cell 117Antigens 311, 323

Host resistance 419Host response 221

Antigenic hybrid 27,39 Human chorionic gonadotropin 373Antigenic site 27Ascaris scum 333Association 231

Human fallopian tubeHybrid 27, 39Hydrogen peroxide 199

373

Bacillus anthracis, non-toxigenic 183 IgA 373Immunity 149, 385, 419Bacillus subtilis 159

Bacterial attachment 137 Immunodeficient mice 363Bacterial fimbriae 429 Immunological adjuvants 159Bacterial invasion 137 Immunoprotection 281

Implant 443Infection 419Infection clearance 343Influenza 105Interferon 105,419Interleukin 1

385Interleukin 2 363Internalisation 231Intracellular 297

Bactericidal activityBasal laminae 209Bordetella pertussisBorrelia burgdorferiCapsule 231Carbohydrate analysis

261

3931 37

351Cationic protein 209Chamber 443Chemiluminescence 173Chemotaxis 173,451

465

Intraepithelial lymphocytes 343Lamina propria lymphocytes 343LD50 493Lectin binding proteins 465Legionella pneumophila 11Leptospira interrogans serovarpomona 311

Chicken 271Chimera 27Chlamydia trachomatisCloning 47, 481ComplementCytokine 105

261Leukotoxin neutralization 411

Cytotoxicity testing, in vitro 405DNA long range mapping 127

Lipase 173Lipopolysaccharide 47, 93, 323

dsRNA 105E. coli 271, 429, 481

Lipopolysaccharide biosynthesis 351Listeria monocytogenes 385

E. coli 0'- 351 Low-Cal response 87E. coli 06 127 LPS-protein-complex 93E. coli K12ELISPOT

47117

137, 231

LPS-release 93Macrophage 199

Endothelial cells Molecular hybridization 429Monoclonal antibody 271,411Eosinophils 333

Epidemiology 127Extracellular enzyme activity 405

Monocyte 173Mouse 183, 297, 385

Extraintestinal isolates 127 Mouse, immunodeficient 363Fallopian tube 373fim genes 393, 487Fimbriae 271, 393, 429

Mouse test 247Multiple copy 475Mutant, non-hemolytic 281

xii

Oral vaccination 117Outer membrane 311Overexpression 459Parasitic 333

Subject Index

Sialylation 323Spleen 297Splenomegaly 493Stability 39

Pasteurella haemolytica 411 Staphylococcus aureus 443Pathogenesis 11Pathogenicity 71Pertussis toxin 159

Staphylococcus epidermidis 443Strain difference 247Streptococcus pyogenesSubunit purification

209Phenotypic characteristics 281 15Plasmid 61,183 Subunit S1 of pertussis toxin 159

343Plasmid, virulence 247, 459, 493 Suppressor/cytotoxic T lymphocytesPoliovirus 27, 39, 419 Surface array protein

Synergism 419T cells, helper/inducer

71Poly-o-glutamic acid capsule 183Polymorphonuclear leukocytes 451Polyriboinosinic acid -polyribocytidylic acid 105

343T lymphocytes, suppressor/cytotoxic 343

Positive regulator 459Prostaglandin 105Protective immunity for pertussis 159

tdh gene 61, 165Temperature regulation 81Thermostable direct hemolysin 165

Protein, cationic 209Protein, secreted 159,311

Tn5 mutagenesis 493Tn5tagging 247

Protein, surface array 71Pseudomonas aeruginosa 173

Toxin, pertussis 159Transcription 459

183pX02 183 Transposon mutagenesisTrophozoite load 343Recombinant protein secretion in Bacillus subtilis

159 Typhimurium 247Recombinant proteins as vaccine 159 Typhoid fever 117,149

Urease 15Recombinant vaccinia virusRegulation 393Resistance 385, 419Respiratory infection 221

363Vaccination, oral 117Vaccines 149, 159Vaccinia virus 363Varicella-zoster virus 451Vibrio cholerae non-01 61Vibrio parahaemolyticus 61, 165

Reticuloendothelial system 297Retrovirus 1rfa 81rfb 81rfb gene cluster 351rfb region 47

vir : :Tn5 493Virulence 199,281Virulence expression 247

S layers 71Salmonella 487Salmonella abortus equi 93

Virulence factors 127

459, 493Virulence genes 481Virulence plasmid 247,

Salmonella aroA 149Salmonella choleraesuis 459Salmonella typhi Ty21 a 117Salmonella typhimurium 287, 487, 493

Virulence regulon 87Virus, herpes 1,253Virus, vaccinia 363Virus, varicella-zoster 451

Secreted proteins 159,311 Yersinia enterocolitica 47, 81Secretory immunity 117Serum resistance 323

Yersiniae 87