Number 1, January 1988

Mini-reviewGenetic determinants of the virulence and infectivity of La Crosse

virus, F . Gonzalez-Scarano, B . Beaty, D . Sundin, R . Janssen, M . J . Endres andN . Nathanson 1

ArticlesUnexpected effects of absorbed normal rabbit serum and bovine serum

on survival of Haemophilus influenzae type b in the infant rat, M. R. Loeb

9

Pulmonary secretions accelerate the metabolic rate of Escherichia coil.R. T . Ellison III and T . J . Giehl 15

Priming and boosting of the rabbit intestinal immune system with liveand killed, smooth and rough Vibrio cholerae cells, W. H. Jansen,H . Gielen, S . G . T. Rijpkema and P . A . M . Guinee 21

Neisseria meningitidis group B serosubtyping using monoclonalantibodies in whole-cell Elisa, H . Abdillahi and J . T . Poolman27

Purification and immunological characterization of neuraminidaseproduced by Streptococcus pneumoniae, R . A. Lock, J . C. Paton andD. Hansman 33

Comparison of clonal analysis and DNA restriction analysis for typing ofNeisseria meningitidis, T . Olyhoek, B . A. Crowe, R . A . Wall and M . Achtman 45

Pathogenesis and genetic control of resistance to the Sterne strain ofBacillus anthracis, S . L. Welkos and A . M . Friedlander53

Antigenic relatedness of a strongly immunogenic 65 kDA mycobacterialprotein antigen with a similarly sized ubiquitous bacterial commonantigen, J . E . R . Thole, P . Hindersson, J . de Bruyn, F . Cremers, J . van der Zee,H . de Cock, J . Tommassen, W. van Eden and J . D . A. van Embden71

Number 2, February 1988Mini-reviewMechanisms of persistence in human virus infections, F. Rapp and

J. M. Cory 85

ArticlesHost factors in the resistance of newborn mice to K1 Escherichia coli

infection, G. Pluschke and S. Pelkonen 93

A new heat-labile cytolethal distending toxin (CLDT) produced byEscherichia coli isolates from clinical material, W. M . Johnson and H . Lior 103

A new heat-labile cytolethal distending toxin (CLDT) produced byCampylobacter spp., W . M. Johnson and H . Lior 115

iii

Inhibition of protein synthesis by a Vero toxin (VT2 or Shiga-like toxinII) produced by Escherichia coli 0157:H7 at the level of elongation factor1-dependent aminoacyl-tRNA binding to ribosomes, T . Ogasawara, K . Ito,K. Igarashi, T. Yutsudo, N . Nakabayashi and Y . Takeda127

Effect of co-incubation with cytomegalovirus on growth of interleukin2-dependent lymphocytes, K. Numazaki, H . Goldman and M . A . Wainberg 137

Two thymidine kinase deficient herpes simplex viruses exhibitunexpected virulence properties, K. M. Izumi and J . G . Stevens145

Evidence of correlation between 50-kilobase plasmid of Salmonellacholeraesuis and its virulence, K . Kawahara, Y . Haraguchi, M . Tsuchimoto,N . Terakado and H . Danbara 155

Short communicationEffects of Interferon-y treatment on surgically simulated wound

infection in mice, M . J . Hershman, G . Sonnenfeld, B . W. Mays, F . Fleming,L. S . Trachtenberg and H . C . Polk, Jr 165

Number 3, March 1988Mini-reviewPathogenesis of measles immunodeficiency and encephalomyelitis :

parallels to AIDS . R . T . Johnson, D . E . Griffin and T . R . Moench169

ArticlesThe interaction of Escherichia coil with normal human serum: factors

affecting the capacity of serum to mediate lipopolysaccharide release .V. L . Tesh and D . C. Morrison 175

The effects of host age, virus dose, and virus strain on heterologousrotavirus infection of suckling mice . R. F . Ramig 189

Experimental meningococcal meningitis in the infant rat . K. Saukkonen

203

Ultrastructural localization of gonococcal antigens in infectedepithelial cells as visualized by post-embedding immuno-electromico-scopy. J . F . L . Weel and P . M. van Putten 213

Oligomerization of 3H-labelled staphylococcal alpha-toxin and frag-ments on adrenocortical Y1 tumour cells . L. Blomqvist and M . Thelestam . . . 223

Investigation of minor components of Escherichia coliType 1 Fimbriae :protein chemical and immunological aspects . K. A . Krogfelt and P . Klemm

231

iv

Number 4, April 1988

Mini-reviewComplex gene expression of lentiviruses . J . L. Davis and J . E. Clements 239

ArticlesNutritionally variant streptococci develop ultrastructural abnormalities

during experimental endocarditis . C. Frehel, R . Hellio, A-C . Cremieux,A. Contrepois and A . Bouvet 247

Morphological studies on fimbriae expressed by Vibrio cholerae 01 .R . H . Hall, P . A . Vial, J . B . Kaper, J . J . Mekalanos and M . M . Levine257

A sandwich adhesin on Streptococcus pneumoniae attaching tohuman oropharyngeal epithelial cells in vitro . B . Andersson, E . H . Beachey,A. Tomasz, E . Tuomanen and C . Svanborg-Eden 267

Outer membrane proteins of Bacteroides fragilis and Bacteroidesvulgatus in relation to iron uptake and virulence . B R. Otto, A . M . J . J .Verweij-van Vught, J . van Doorn and D . M. Maclaren 279

Gonococcal variants selected by growth • in vivo or in vitro haveantigenically different LPS . R . D. de Hormaeche, H . Jessop and K. Senior 289

Short communicationsDifferential susceptibility of salmonid fishes to furunculosis correlates

with differential serum enhancement of Aeromonas salmonicida extra-cellular protease activity . A. E . Ellis and K . J . Stapleton299

An experimental mouse model of Yersinia-induced reactive arthritis .Z. Yong, J . L . Hill, T . Hirofuji, M . Mander and D . T . Y . Yu305

Purification of fimbriae from Pasteurella haemolytica A-1 . A. A . Potter,K. Ready and J . Gilchrist 311

Number 5, May 1988Mini-review

Clostridium perfringens enterotoxin. B . A. McClane, P . C. Hanna andA. P. Wnek 317

ArticlesPoxvirus deletion mutants :virulence and immunogenicity . K. M. Edwards,

T . C . Andrews, J . Van Savage, P . S. Palmer and R . W. Moyer325

Cloning of the adenylate cyclase genetic determinant of Bordetellapertussis and its expression in Escherichia coli and B.pertussis .R . M . Brownlie, J . G . Coote, R . Parton, J. E. Schultz, A. Rogel and E . Hanski 335

Nucleotide sequence of the invasion plasmid antigen B and C genes(ipaB and ipaC) of Shigella flexneri. B . Baudry, M . Kaczorek andP. J. Sansonetti 345

v

Bacteriophage T4 resistant mutants of the plant pathogen Erwiniacarotovora. M . Pirhonen, P . Heino, I . Helander, P . Harju and E . T. Palva 359

Mutations in cloned Escherichia coli P fimbriae genes that makesfimbriae-production resistant to suppression by trimethoprim .V. Vaisanen-Rhen, S . Saarela and M . Rhen 369

Short communicationsHemagglutination by Staphylococcus saprophyticus and other

coagulase-negative staphylococci . J . Beuth, H . L. Ko, F. Schumacher-Perdreau, G . Peters, P . Heczko and G . Pulverer 379

Plasmid-associated virulence of Salmonella enteritidis. M . Hovi,S. Sukupolvi, M . F . Edwards and M . Rhen 385

Number 6, June 1988Mini-review

Structural and functional modulation of gonococcal surfaceproteins . C. Gibbs, R . Haas and T . F . Meyer 393

ArticlesFunctional and structural alterations of the nervous system

induced by avian retrovirus RAV-7 . L . R . Whalen, D . W. Wheeler, D . H .Gould, S. A. Fiscus, L . C. Boggie and R . E . Smith 401

Pathogenesis of acute myocardial necrosis in inbred mice infectedwith coxsackievirus B3. J . B . Grun, M. Schultz, S . D. Finkelstein, R . L .Crowell and B . J . Landau 417

Yersinia enterocolitica 0 :9 as a potential live oral carrier forprotective antigens . M .-P . Sory and G. Cornelis

431

Cytotoxic effects of ingested Staphylococcus aureus on bovineendolethial cells : Role of S.aureus a-hemolysin . J. M . Vann and R . A .Proctor 443

Short communicationProduction of a Shiga-like cytotoxin by Campylobacter. M. A.

Moore, M. J . Blaser, G . I . Perez-Perez and A . D. O'Brien455

Volume contents and index to volume 4 i

vi

Index to Volume 4Author Index

Abdillahi, H . and Poolman, J . T . Neisseria meningitidis group B serosubtyping using monoclonal antibodies inwhole-cell Elisa 27

Achtman, M . See Olyhoek, T. 45Andersson, B ., Beachey, E . H ., Tomasz, A ., Tuomanen, E . and Svanborg-Eden, C. A sandwich adhesin on

Streptococcus pneumoniae attaching to human oropharyngeal epithelial cells in vitro 267Andrews, T . C . See Edwards, K . M. 325Baudry, B., Kaczorek, M . and Sansonetti, P . J . Nucleotide sequence of the invasion plasmid antigen B and C

genes (ipaB andipaC) ofShigella flexneri 345Beachey, E . H . See Andersson, B . 267Beaty, B . See Gonzalez-Scarano, F . 1Beuth, J ., Ko, H . L., Schumacher-Perdreau, F ., Peters, G ., Heczko, P. and Pulverer, G . Hemagglutination .by

Staphylococcus saprophyticus and other coagulase-negative staphylococci 379Blaser, M . J . See Moore, M . A. 455Blomqvist, L . and Thelestam, M . Oligomerization of 3 H-labelled staphylococcal alpha-toxin and fragments on

adrenocortical Yl tumour cells 223Boggle, L. C. See Whalen, L . R . 401Bouvet, A . See Frehel, C . 247Brownlie, R. M ., Coote, J . G ., Parton, R ., Schultz, J . E ., Rogel, A . and Hanski, E . Cloning of the adenylate

cyclase genetic determinant of Bordetella pertussis and its expression in Escherichia coil and B. pertussis 335Clements, J . E. See Davis, J . L. 239Contrepois, A. See Frehel, C . 247

Davis, J . L . and Clements, J . E . Complex gene expression of lentiviruses 239de Bruyn, J . See Thole, J . E. R . 71de Cock, H . See Thole, J . E . R . 71de Hormaeche, R . D ., Jessop, H . and Senior, K. Gonococcal variants selected by growth in vivo or in vitro have

antigenically different LPS 289Edwards, K. M., Andrews, T. C ., Van Savage, J ., Palmer, P . S . and Moyer, R . W. Poxvirus deletion mutants :

virulence and immunogenicity 325Edwards, M . F. See Hovi, M . 385Ellis, A . E . and Stapleton, K . J . Differential susceptibility of salmonid fishes to furunculosis correlates with

differential serum enhancement of Aeromonas salmonicida extracellular protease activity 299Ellison, R . T ., III and Giehl, T . J . Pulmonary secretions accelerate the metabolic rate of Escherichia coil 15Endres, M . J . See Gonzalez-Scarano, F. 1Finkelstein, S. D . See Grun, J . B. 417Fiscus, S. A. See Whalen, L. R . 401Fleming, F . See Hershman, M . J . 165Frehel, C ., Hellio, R ., Cremieux, A-C., Contrepois, A. and Bouvet, A . Nutritionally variant streptococci develop

ultrastructural abnormalities during experimental endocarditis 247Friedlander, A. M . See Welkos, S . L . 53Gibbs, C ., Haas, R . and Meyer, T. F . Structural and functional modulation of gonococcal surface proteins 391;Giehl, T. J . See Ellison, R . T., III 15Gielen, H . See Jansen, W . H . 21Gilchrist, J . See Potter, A. A . 311Goldman, H . See Numazaki, K . 137Gonzalez-Scarano, F., Beaty, B., Sundin, D., Janssen, R ., Endres, M. J. and Nathanson, N . Genetic

determinants of the virulence and infectivity of La Crosse virus 1Gould, D . H . See Whalen, L. R. 401Griffin, D . E. See Johnson, R . T . 169Grun, J . B ., Schultz, M ., Finkelstein, S. D ., Crowell, R . L. and Landau, B . J . Pathogenesis of acute myocardial

necrosis in inbred mice infected with coxsackievirus B3 417Guinee, P . A. M . See Jansen, W. H . 21

vii

Coote, J . G . See Brownlie, R . M . 335Cornelis, G . See Sory, M.-P . 431Cory, J . M . See Rapp, F. 85Cremers, F. See Thole, J . E . R . 71Cremieux, A-C. See Frehel, C . 247Crowe, B. A. See Olyhoek, T. 45Crowell, R . L. See Grun, J . B . 417Danbara, H. See Kawahara, K. 155

VIII

Authorlndex

Haas, R. See Gibbs, C . 393Hall, R . H ., Vial, P . A ., Kaper, J . B ., Mekalanos, J . J . and Levine, M . M . Morphological studies on fimbriae

expressed by Vibrio cholerae 01 257Hanna, P . C . See McClane, B . A. 317Hanski, E. See Brownlie, R . M . 335Hansman, D. See Lock, R . A. 33Haraguchi, Y. See Kawahara, K . 155Harju, P. See Pirhonen, M. 359Heczko, P. See Beuth, J . 379Heino, P. See Pirhonen, M. 359Helander, I . See Pirhonen, M . 359Hellio, R . See Frehel, C . 247Hershman, M . J ., Sonnenfeld, G ., Mays, B . W., Fleming, F ., Trachtenberg, L. S . and Polk, H . C., Jr Effects of

interferon -7 treatment on surgically simulated wound infection in mice 165Hill, J . L . See Yong, Z. 305Hindersson, P. See Thole, J . E . R . 71Hirofuji, T . See Yong, Z. 305Hovi, M., Sukupolvi, S ., Edwards, M . F . and Rhen, M . Plasmid-associated virulence of Salmonella enteritidis

385Igarashi, K. See Ogasawara, T. 127Ito, K . See Ogasawara, T. 127lzumi, K. M . and Stevens, J . G . Two thymidine kinase deficient herpes simplex viruses exhibit unexpected

virulence properties 145Jansen, W . H ., Gielen, H ., Rijpkema, S . G . T . and Guinea, P . A . M . Priming and boosting of the rabbit intestinal

immune system with live and killed, smooth and rough Vibrio cholerae cells 21Janssen, R . See Gonzalez-Scarano, F . 1Jessop, H. See de Hormaeche, R . D. 289Johnson, W. M . and Lior, H . A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli

isolates from clinical material 103Johnson, W . M . and Lior, H . A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter

spp. 115Johnson, R . T., Griffin, D. E. and Moench, T . R. Pathogenesis of measles immunodeficiency and

encephalomyelitis : parallels to AIDS 169Kaczorek, M. See Baudry, B . 345Kaper, J . B . See Hall, R . H . 257Kawahara, K., Haraguchi, Y. Tsucimoto, M ., Terakado, N . and Danbara, H . Evidence of correlation between 50

kilobase plasmid of Salmonella choleraesuis and its virulence 155Klemm, P. See Krogfelt, K . A. 231Ko, H . L . See Beuth, J . 379Krogfelt, K . A. and Klemm, P. Investigation of minor components of Escherichia coli Type 1 fimbriae : protein

chemical and immunological aspects 231Landau, B . J . See Grun, J . B . 417Levine, M . M . See Hall, R . H . 257Lior, H. See Johnson, W. M. 103,115Lock, R . A., Paton, J . C. and Hansman, D . Purification and immunological characterization of neuraminidase

produced by Streptococcus pneumoniae 33Loeb, M. R . Unexpected effects of absorbed normal rabbit serum and bovine serum on survival of Haemophi/us

influenzae type b in the infant rat 9McClane, B . A ., Hanna, P . C . and Wnek, A. P . Clostridium perfringens enterotoxin 317Maclaren, D. M . See Otto, B . R. 279Mander, M. See Yong, Z . 305Mays, B . W. See Hershman, M . J. 165Mekalanos, J . J . See Hall, R . H . 257Meyer, T . F . See Gibbs, C. 393Moench, T . R . See Johnson, R. T. 169Moore, M . A ., Blaser, M . J ., Perez-Perez, G . I . and O'Brien, A. D . Production of a Shiga-like cytotoxin by

Campylobacter 455Morrison, D . C . See Tesh, V . L. 175Moyer, R . W . See Edwards, K . M . 325Nakabayashi, N. See Ogasawara, T. 127Numazaki, K., Goldman, H . and Weinberg, M . A . Effect of co-incubation with cytomegalovirus on growth of

interleukin 2-dependent lymphocytes 137O'Brien, A . D . See Moore, M. A. 455Ogasawara, T ., Ito, K., Igarashi, K ., Yutsudo, T ., Nakabayashi, N . and Takeda, Y . Inhibition of protein synthesis

by a Vero toxin (VT2 or Shiga-like toxin II) produced by Escherichia coli 01 57 :H7 at the level of elongationfactor 1 -dependent aminoacyl-tRNA binding to ribosomes 127

Olyhoek, T., Crowe, B . A ., Wall, R . A. and Achtman, M . Comparison of clonal analysis and DNA restrictionanalysis for typing of Neisseria meningitidis 45

Authorindex

ix

Otto, B . R ., Verweij-van Vught, A . M . J . J ., van Doorn, J . and Maclaren, D. M. Outer membrane proteins ofBacteroides fragilis and Bacteroides vulgatus in relation to iron uptake and virulence 279

Palmer, P . S . See Edwards, K. M. 325Palva, E . T. See Pirhonen, M. 359Parton, R . See Brownlie, R . M. 335Paton, J . C. See Lock, R . A. 33Pelkonen, S. See Pluschke, G. 93Perez-Perez, G . I . See Moore, M . A . 455Peters, G. See Beuth, J . 379Pirhonen, M., Heino, P ., Helander, I ., Harju, P . and Palva, E. T. Bacteriophage T4 resistant mutants of the plant

pathogen Erwinia carotovora 359Pluschke, G. and Pelkonen, S . Host factors in the resistance of newborn mice to K1 Escherichia coli infection

93Polk, H . C ., Jr See Hershman, M . J . 165Poolman, J . T . See Abdillahi, H . 27Potter, A . A., Ready, K . and Gilchrist, J . Purification of fimbriae from Pasteurella haemolytica A-1 311Proctor, R . A . See Vann, J . M ., 443Pulverer, G . See Beuth, J . 379Ramig, R . F. The effects of host age, virus dose, and virus strain on heterologous rotavirus infection of suckling

mice 189Rapp, F . and Cory, J . M . Mechanisms of persistence inhuman virus infections 85Ready, K . See Potter, A . A. 311Rhen, M. See Hovi, M . 385Rhen, M. See Vaisainen-Rhen, V . 369Rijpkema, S . G . T. See Jansen, W . H . 21Rogel, A . See Brownlie, R . M. 335Saarela, S . See Vaisanen-Rhen, V. 369Sansonetti, P. J . See Baudry, B. 345Saukkonen, K . Experimental meningococcal meningitis in the infant rat 203Schultz, J . E . See Brownlie, R . M . 335Schultz, M . See Grun, J . B. 417Schumacher- Perdreau, F . See Beuth, J. 379Senior, K . See de Hormaeche, R . D. 289Smith, R . E . See Whalen, L . R . 401Sonnenfeld, G. See Hershman, M . J . 165Sory, M.-P. and Cornelis, G . Yersinia enterocolitica 0:9 as a potential live oral carrier for protective antigens

431Stapleton, K. J . See Ellis, A . E . 299Stevens, J . G . See Izumi, K . M . 145Sukupolvi, S . See Hovi, M. 385Sundin, D . See Gonzalez-Scarano, F . 1Svanborg-Eden, See Andersson, B . 267Takeda, Y. See Ogasawara, T. 127Terakado, N. See Kawahara, K. 155Tesh, V. L . and Morrison, D . C . The interaction of Escherichia coil with normal human serum : factors affecting

the capacity of serum to mediate lipopolysaccharide release 175Thelestam, M . See Blomqvist, L . 223Thole, J . E . R ., Hindersson, P ., de Bruyn, J ., Cremers, F ., van der Zee, J ., de Cock, H ., Tommassen, J ., van Eden,

W. and van Embden, J. D . A. Antigenic relatedness of a strongly immunogenic 65 kDA mycobacterialprotein antigen with a similarly sized ubiquitous bacterial common antigen 71

Tomasz, A. See Andersson, B . 267Tommassen, J . See Thole, J . E . R . 71Trachtenberg, L. S. See Hershman, M . J . 165Tsuchimoto, M. See Kawahara, K. 155Tuomanen, E . See Andersson, B . 267Vaisanen-Rhen, V., Saarela, S . and Rhen, M . Mutations in cloned Escherichia coil P fimbriae genes that makes

fimbriae-production resistant to suppression by trimethoprim 369van der Zee, J. See Thole, J . E . R . 71van Doorn, J . See Otto, B . R. 279van Eden, W. See Thole, J . E . R . 71van Embden, J. D . A. See Thole, J . E . R . 71van Putten, P. M . SeeWeel, J . F . L. 213Van Savage, J . See Edwards, K. M . 325Vann, J . M . and Proctor, R . A . Cytotoxic effects of ingested Staphylococcus aureus on bovine endothelial cells :

Role of S. aureus x-hemolysin 443Verweij-van Vught, A. M . J . J . See Otto, B . R . 279Vial, P . A . See Hall, R . H. 257Wainberg, M . A. See Numazaki, K . 137

x

Author Index

Wall, R . A. See Olyhoek, T . 45Wee[, J . F . L. and van Putten, P . M . Ultrastructural localization of gonococcal antigens in infected epithelial

cells as visualized by post-embedding immuno-electronmicroscopy 213Welkos, S . L . and Friedlander, A . M . Pathogenesis and genetic control of resistance to the Sterne strain of

Bacillus anthracis 53Whalen, L. R ., Wheeler, D . W., Gould, D . H ., Fiscus, S . A., Boggie, L . C . and Smith, R . E. Functional and

structural alterations of the nervous system induced by avian retrovirus RAV-7 401Wheeler, D . W . See Whalen, L . R. 401Wnek, A . P . See McClane, B . A. 317Yong, Z., Hill, J . L., Hirofuji, T ., Mander, M . and Yu, D . T. Y . An experimental mouse model of Yersinia-induced

reactive arthritis 305Yu, D . T . Y . See Yong, Z . 305Yutsudo, T . See Ogasawara, T. 127

Subject index 1ci

Subject IndexAdenylate cyclase 335

Genetic determinants 1Gold-labelling 213

Adherence 257

299

Gonococcal surface proteins 393Adhesins 231, 267 Gonococcus 289Adhesion 213, 267 Growth conditions 279

Haemagglutination 311, 369, 379Aeromonas salmonicidaAIDS 169 Haemolysin 335,443

Haemophilus influenzae 9Heart muscle disease 417HeLa 115cC-hemolysin 443HEp-2 115Hepatitis 417Herpes simplex virus 145Heterologous infection 189Host age-dependence 189IL-2 137Immunization 431Immunodeficiency 169Immunohistochemistry 401Infant rat model 9, 203Infection 85, 289Interferon 165Internalization 213Intranasal virulence 335

Anthrax 53Antibody 27, 289, 311Antigen 289Antigen preservationArthritis 71, 305Avian leukosis viruses

213

401Bacillus anthracis 53Bacteremia 155Bacterial adhesins 231Bacterial antigen 71Bacterial cell surface 175Bacterial clone 45Bacterial metabolism 15Bacteriophage T4 359Bacteroides fragilis 279Bacteroides vulgatus 279BALB/c mice 417Bordetella pertussis 335Bronchoalveolar lavage fluidsCampylobacter 455Campylobactercytotoxin 455

15 Invasion genes 345Iron 279K1 E. coli 93

C3H/HeJ mice 417Chang epithelial cellsCHO 115

Kinetics of bacteraemia and meningitis 203213 Klebsiella pneumoniae 165

La Crosse virus 1Lentiviruses 239Lipopolysaccharide 175,359Live carrier 431Live and inactivated vaccine 21

Cholera-like enterotoxin 115CLDT 103,115Cloning 335Clostridium perfringens 317CNS disease 401Colonization 257

Live vaccine 53LPS 289

Common bacterial antigen 71 LPS mutants 359LT 103Competence factor 267

Complement 53Coxsackievirus B3 417Cultured Yl cells 223Cytomegalovirus 137

Measles 169Membrane damage 223Meningitis 93Meningococcal group B infection 203Meningoencephalitis 401Cytotoxin 103, 115, 443, 455

DNA sequence 345 Mice 53, 417DNA restriction pattern 45Electron microscopy 247, 311

Monoclonal antibodies 27,311Mouse experimental model 189, 305Myocardial necrosis, acute 417Elisa, whole-cell 27

Encephalomyelitis 169Endocarditis, experimental 247

Myocardiotropic virus variants 417Neisseria gonorrhoeae 213Neisseria meningitidis 27,45Neonatal sepsis and meningitis 93Neuraminidase 33Neuroinvasiveness 145Neuropathology 401Neurovirulence 145

Endothelial cells 443Endotoxin 93Enterotoxin 317Epidemiology 45Erwinia 359E. coli 15, 93, 175, 231E. coli Shiga-like toxinExpression 335Extracellular proteaseFimbriae 257, 311Fimbriae, P. 369

455

299

Nutritionally variant streptococci 247Oligomers 223Organelle structure 231Outer membrane proteins 27, 279P fimbriae 369

Fimbrial components, minor 231 Pasteurella haemolytica 311Pathogenesis 53Persistent virus infections 85Phagocytosis 443Phytopathogen 359Pili fimbriae 257Plasmids 385

fl-galactosidase 431Gastroenteritis 189Gene expression 239Genes, invasion 345Gene regulation 369Genetic control 53

xii

Pneumococcus 33, 267Post-embedding immuno-electronmicroscopy

Subject index

213T lymphocytes 137Thymidine kinase 145

Post-vaccinial encephalitisPoxvirus 325

325 Toxin fragments 223Toxin, Shiga-like 127,455

Protease enhancement by serum 299 Toxin, Vero 115,127a -toxin 223Trimethoprim 369

Protease inhibition 299Protection 21Protein synthesis inhibition 127 Vaccine 431

Vaccine, live 53Rabbit model 21Rabbitpox virus 325 Vaccine, live and inactivated 21Recombinant mycobacterial antigen 71 Vaccine vectors 325Resistance, natural 93Retrovirus 401Rotavirus 189

Vaccinia 325Vero 115,127Vibrio cholerae 21, 257

Salmonella choleraesuis 155 Virulence 279Salmonella enteritidis 385Salmonid fish 299

Virulence, intranasal 335Virulence, plasmid 155

385Virulence, plasmid-associatedSerogroup A 45Serotyping 27Serum albumin effects 9

Virus dosage effects 189Virus infections, persistent 85 •Virus strain effects 189Serum-mediated LPS release 175

Shiga-like toxin 127,455455

Viruses, avian leukosis 401Viruses, Coxsackie B3 417Viruses, herpes simplex 145

ShigellaShigella flexneris 345Smooth and rough 21Staphylococci 379Staphylococcus aureus 223,443

Viruses, La Crosse 1Viruses, pox 325Viruses, rabbit pox 325VT2 127Staphylococcus saprophyticus 379

Sterne 53Streptococci 247

Yersinia 305Yersinia enterocolitica 431Whole-cell Elisa 27Wound infection 165

Streptococcus pneumoniae 33Subtyping 27Surface proteins, gonococcal 393