Warm-UpWarm-Up

1.1. What types of substances need to move What types of substances need to move into and out of cells?into and out of cells?

2.2. What cellular organelle facilitates this What cellular organelle facilitates this movement?movement?

Show Us What You Can Do!Show Us What You Can Do! With a partner…grab a mini white board With a partner…grab a mini white board

(and a WB marker), and design an (and a WB marker), and design an illustration that explains active and passive illustration that explains active and passive transport…you can’t use the picture that I transport…you can’t use the picture that I drew…but you can be creative and or drew…but you can be creative and or humorous humorous

Be prepared to explain what makes your Be prepared to explain what makes your illustration effective. You will do this with illustration effective. You will do this with another pair of illustrators.another pair of illustrators.

You should be prepared to offer and accept You should be prepared to offer and accept constructiveconstructive criticism. criticism.

Lab: Cell Transport-OsmosisLab: Cell Transport-OsmosisPre-Lab QuestionsPre-Lab Questions

1.1. Why is it necessary for materials to Why is it necessary for materials to move move intointo a cell? What types of a cell? What types of substances are these?substances are these?

2.2. Why is it necessary for materials to Why is it necessary for materials to move move out ofout of a cell? What types of a cell? What types of substances are these?substances are these?

3.3. What is the limiting factor that is the What is the limiting factor that is the reason that cells are not bigger than reason that cells are not bigger than they are?they are?

4.4. What is osmosis?What is osmosis?

Some Important Pre-Lab Some Important Pre-Lab VocabularyVocabulary

Hypertonic:Hypertonic: the cell’s environment has a the cell’s environment has a higherhigher concentration of solutes (stuff) concentration of solutes (stuff) than inside the animal or plant cell. than inside the animal or plant cell.

HypotonicHypotonic: the cell’s environment has a : the cell’s environment has a lowerlower concentration of solutes (stuff) than concentration of solutes (stuff) than inside the animal or plant cell. inside the animal or plant cell.

IsotonicIsotonic: the cell’s environment has the : the cell’s environment has the samesame concentration of solutes (stuff) concentration of solutes (stuff) than inside the animal or plant cell. than inside the animal or plant cell.

What You’ll Be DoingWhat You’ll Be Doing

Over the course of the next several days, Over the course of the next several days, you will be immersing an egg in various you will be immersing an egg in various solutions.solutions.

Prior to each immersion, you will hypothesize as Prior to each immersion, you will hypothesize as to what will happen to the egg.to what will happen to the egg.

On each day that you perform this task, On each day that you perform this task, you will make detailed quantitative and you will make detailed quantitative and qualitative observations about the egg. qualitative observations about the egg. You will also record qualitative You will also record qualitative observations regarding the solution used.observations regarding the solution used.

For ExampleFor Example On day one you will immerse the egg in 5% On day one you will immerse the egg in 5%

CHCH33COOH (Vinegar).COOH (Vinegar). Your day 1 Hypothesis will be something like: Your day 1 Hypothesis will be something like: I I

think that if the egg is immersed in 5% think that if the egg is immersed in 5% CHCH33COOH for one to two days, then …”.COOH for one to two days, then …”.

You will record the circumference of the egg You will record the circumference of the egg using the string providedusing the string provided

You will record the mass of the egg using the You will record the mass of the egg using the analytical balance.analytical balance.

You will record the color, texture, and any other You will record the color, texture, and any other observable traits of the egg.observable traits of the egg.

You will record your observations You will record your observations regarding the solutionregarding the solution as well (clear, red, as well (clear, red, cloudy, bubbles present, viscous (thick), cloudy, bubbles present, viscous (thick), sweet smelling, etc.).sweet smelling, etc.).

Osmosis Lab:Osmosis Lab:MaterialsMaterials

1.1. 1 Egg1 Egg

2.2. White Vinegar (aka: White Vinegar (aka: acetic acid or acetic acid or ethanoic acid or…ethanoic acid or…CHCH33COOH)COOH)

3.3. Analytical BalanceAnalytical Balance

4.4. Ruler (metric)Ruler (metric)

5.5. StringString

6.6. HH22O (water)O (water)

7. Corn Syrup7. Corn Syrup

8. Food Coloring8. Food Coloring

9. Plastic Cup9. Plastic Cup

10. NaCl (salt)10. NaCl (salt)

*11. One liquid (safe) of *11. One liquid (safe) of your choiceyour choice

*If time permits*If time permits

ProcedureProcedure

1.1.Obtain one egg and one plastic cup. Write your Obtain one egg and one plastic cup. Write your name on a label and place it on the side of cup!name on a label and place it on the side of cup!2.2.Record your Day 1 hypothesis…you will be Record your Day 1 hypothesis…you will be placing the egg in vinegar. If….then...placing the egg in vinegar. If….then...3.3.Use the analytical balance to mass your egg to Use the analytical balance to mass your egg to the nearest 0.1 (1/10) gram.the nearest 0.1 (1/10) gram.4.4.Use the string and a metric ruler to measure the Use the string and a metric ruler to measure the circumference of your egg in centimeters to the circumference of your egg in centimeters to the nearest 0.1 (1/10) cm.nearest 0.1 (1/10) cm.5.5.Record any additional Record any additional qualitativequalitative observations observations about your egg (color, texture, markings, etc.)about your egg (color, texture, markings, etc.)

6. Gently place your egg in the plastic cup 6. Gently place your egg in the plastic cup and carefully add the 5 % acetic acid and carefully add the 5 % acetic acid solution (vinegar) until your egg is solution (vinegar) until your egg is covered. covered.

7. Observe for at least five (5) minutes and 7. Observe for at least five (5) minutes and record any additional observations.record any additional observations.

8. Place your cup in the designated area 8. Place your cup in the designated area labeled with your class color.labeled with your class color.