Ways of Studying Cells A. Visualization of Cells
BSCI 420 Advanced Cell Biology Sept 11, 2002
“Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln
(In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath)
Resolving Power
Bright-fieldlight microscope
Resolution depends on the Numerical Aperture
Types of LM: A) Bright field B) Phase contrast
Types of LM: C) Diff. Interference Contrast
D) Dark field
Bright field microscopy of stained section of kidney tubules
Bright field vs. Phase contrast
Constructive and destructive interference of light
Fluorescence Microscope
Fluorescence image of a cell in mitosis labeled w 3 tags:Green: fluorescein-labeled anti-tubulin (MTS)Red: anti-centromereproteinBlue: DAPI(DNA)
Indirect Immunocytochemistry
Excitation and Emission wave-lengths
Note emission w-l is always > thanexcitation w-l.So they have lost energy(Energy = 1/wave-length
Immunofluorescence of individual MTs
Images of Conventional (A) vs Confocal (B) Fluorescence
(Drosophila embryo stained w fluorescent probe against actin)
GFT tagged talin in trichrome cells of Arabidopsis
Dynamics of GFP-labeled Cajal bodies in the nucleus