CURRICULUM FRAMEWORK AND SYLLABUS FOR OUTCOME BASED EDUCATION IN Bachelor of Biotechnology Degree Program FOR THE STUDENTS ADMITTED FROM THE ACADEMIC YEAR 2019 - 2020 ONWARDS HICAS HINDUSTHAN COLLEGE OF ARTS AND SCIENCE (AUTONOMOUS) (Affiliated to Bharathiar University and Accredited by NAAC) COIMBATORE-641028 TAMILNADU, INDIA. Phone: 0422-4440555 : www.hindusthan.net/hicas / BOS meeting approved: Approved in 5 th Academic Council meeting on :
Transcript
CURRICULUM FRAMEWORK AND SYLLABUSFOR OUTCOME BASED EDUCATION IN
Bachelor of Biotechnology Degree Program
FOR THE STUDENTS ADMITTED FROM THEACADEMIC YEAR 2019 - 2020 ONWARDS
HICAS
HINDUSTHAN COLLEGE OF ARTS AND SCIENCE (AUTONOMOUS)(Affiliated to Bharathiar University and Accredited by NAAC)
COIMBATORE-641028TAMILNADU, INDIA.
Phone: 0422-4440555: www.hindusthan.net/hicas/
BOS meeting approved: Approved in 5thAcademic Council meeting on :
HINDUSTHAN COLLEGE OF ARTS AND SCIENCEDEPARTMENT OF BIOTECHNOLOGY
VISION
To provide world class education to the students to face global challenges and to inculcate the latest
trends in technological advancement. To cater the needs of the environmental and ethical values in the mind of
students to become good citizens and entrepreneurs.
MISSION
The Mission of the college is to pursue a philosophy of perceptual acquisition of knowledge. The
important policy is to provide value based education and to bring out the hidden potentials in students that equip
them to approach life with optimism.
Programme Educational Objectives (PEO)
Under Graduates of B. Sc. Biotechnology program will be
PEO1: Understand and apply the concepts of Biotechnology, instrumentation and related aspects of
science and technology.
PEO2: To produce research oriented Biotechnology graduates who will be employable in academic/Industry sponsored research projects for pursuing higher studies and successful careers in industry.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
PROGRAM OUTCOME
PO1: To identify, analyze and understand problems related to biotechnology and finding, valid conclusions with basic knowledge in biotechnology.
PO2: To gain and apply knowledge of Biotechnology concepts to solve problems related to field of Biotechnology.
PO3: Graduates will be able design, perform experiments, analyze and interpret data for investigating complex problems in biotechnology related fields.
PO4: To undertake any responsibility as an individual and as a team in a multidisciplinary environment.
PROGRAM SPECIFIC OUTCOMES (PSO)
PSO1: To make students competent in the field of biotechnology and its allied areas.
PSO2: Acquire knowledge in domain of biotechnology enabling their applications in industry and research.
PSO3: To make the students to be an effective entrepreneur to start biotechnology industries and or companies.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
HINDUSTHAN COLLEGE OF ARTS AND SCIENCE (AUTONOMOUS)
COIMBATORE – 641 028
B.Sc., BIOTECHNOLOGY
SCHEME OF EXAMINATIONS – CBCS PATTERN
(For the Students Admitted from the Academic Year 2019 -2020 and Onwards)
19BTU17 SEC Allied Practical VII - Programming Lab in C (CSC)
2 3 40 60 100 2
Part IV
19GSU03 AEE Skill Based - Internet Security 2 - 100 - 100 2
Part V
19GSU04 AEE Extension Activity - - 100 - 100 2
28 23Students Should Complete Value Added Courses, Online Courses (Or) Participation Certificates For Seminars, Workshops From Other Institutions For Each Semester And Womens Studies / Interdisciplinary at the end of Second YearExtension Activity – means all those activities under NSS/NCC/sports/YRC Programme and other Co and extracurricular activities offered under part V of the programme. Every student shall participate compulsorily for a period of not less than two years (4 semesters) in any one of these programmes.Semester V
19BTU30 SEC Practical XI -, Environmental Biotechnology
5 6 40 60 100 3
30 24
Students Should Complete Value Added Courses, Online Courses / Entrepreneurship/Startups/ Job Oriented Courses and Placement Training at the end of the Third Year
140
BOS meeting approved: Approved in 5thAcademic Council meeting on :
No of papers Course Type
Total Credit Points
4 Modern Indian Language(MIL) 12
4 Ability Enhancement Compulsory course(AECC) 12
6 Ability Enhancement Elective (AEE) 12
11 Discipline Specific course(DSC) 54
2 Discipline Specific Elective(DSE) 07
12 Skill Enhancement Course(SEC) 30
4 Generic Elective(GE) 13
43 TOTAL 140
BOS meeting approved: Approved in 5thAcademic Council meeting on :
UG- REGULATION (From 2019-2020 and Onwards)
1. Internal Marks for all UG
Components MarksTest I 5Test II 5
Model Exam 10Assignment 5Attendance* 5
TOTAL 30
*Split-up of Attendance Marks for UG
75-79 - 1 marks 80-84 - 2 marks 85-89 - 3 marks 90-94 - 4 marks 95-100 - 5 marks
QUESTION PAPER PATTERN FOR I.E TEST I and IIDuration: Two Hours Maximum: 50 Marks
SECTION - A (6 x 1 = 6 Marks)Answer ALL Questions
ALL Questions Carry EQUAL MarksMultiple choice/Fill up the blanks /True or False questions
SECTION - B (4x 5 = 20 marks)Answer ALL Questions
ALL Questions Carry EQUAL Marks Either or Type
SECTION - C (3x 8 = 24 marks)Answer ALL Questions
ALL Questions Carry EQUAL Marks Either or Type
QUESTION PAPER PATTERN FOR IE Model Examination
Duration: Three Hours Maximum: 70 MarksSECTION - A (10x1=10 Marks)
Answer ALL QuestionsALL Questions Carry EQUAL Marks
Q.No 1 to 10: (Multiple choice/Fill up the blanks /True or False questions).(Two questions from each unit)SECTION - B (5x4=20 Marks)
Answer ALL QuestionALL Questions Carry EQUAL MarksQ.No 11 to 15: Either or type questions
(One question from each Unit)SECTION- C (5x8=40 Marks)
Answer ALL QuestionsALL Questions carry EQUAL Marks
Q.No 16 to 20: Either or type questions (One question from each Unit)2 a) Components for Practical I.E.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Components Marks
Test –I 20
Test - II 20
Total------- 40=====
2 b) Components for Practical E.E.
Components Marks
Completion of Experiments 50
Record 5
Viva 5
Total------- 60=====
3. Institutional/ Industrial Training, Mini Project and Major Project WorkInstitutional /Industrial Training Mini Project Major Project WorkComponents Marks Marks Components Marks
I.EWork Diary Report Viva –voceExamination
255025
- 5050
I. Ea) Attendance 10 Marksb) Review /Work Diary*1 30 Marks
40
Total 100====
100==== E.E*2
a) Final Report 40 Marksb) Viva-voce 20 Marks 60
Total 100====
*1 Review is for Individual Project and Work Diary is for Group Projects (group consisting of minimum 3 and maximum 5)
*2Evaluation of report and conduct of viva voce will be done jointly by Internal and External Examiners
3. Components for Value Education (Part IV):S.No. Components Marks
BOS meeting approved: Approved in 5thAcademic Council meeting on :
a) Attendance 30 marks96% and above - 30 marks91% to 95% - 25 marks86% to 90% - 20 marks76% to 85% - 10 marks
b) Participation in group activity 30 marksc) Assignment (2 x 10) 20 marksd) Test
(1 hr for 20 marks)2 out of three questions, 10 marks each
20 marks
Total 100 marks
On completion of the above components students will be remarked as follows:Range of marks Equivalent remarks80 and above Exemplary70 – 79 Very good60 – 69 Good50 – 59 Fair40 – 49 SatisfactoryBelow 39 Not Satisfactory = Not completed
The passing minimum for this paper is 40%In case, the candidate fails to secure 40% passing minimum, he / she may have to reappear for the same in the subsequent semesters.
5. Guidelines for Environmental Studies (Part IV)
The paper Environmental Studies is to be treated as 100% IE course which is offered in III Semester for II year UG students. The classes will be handled for two hours per week till the end of the Semester. At least one field trip should be arranged.Total Marks for the subject = 100
Components Marks
Two Tests (2 x 30) 60
Field visit and report (10 + 10) 20
Two assignments (2 x 10) 20
Total------- 100=====
The question paper pattern is as follows:Test I – 2 hours [3 out of 5 essay type questions] 3 x 10 = 30 MarksTest II – 2 hours [3 out of 5 essay type questions] 3 x 10 = 30 Marks
The passing minimum for this paper is 40%In case, the candidate fails to secure 40% passing minimum, he / she may have to reappear for the same in the subsequent semesters.6. Guidelines for Skill based subject - Internet Security (Part IV)
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Components Marks
Two Tests (2 x 40) 80
Two assignments (2 x 10) 20
Total------- 100=====
The question paper pattern is as follows:Test I – 2 hours [4 out of 7 essay type questions] 4 x 10 = 40Marks Test II – 2 hours [4 out of 7 essay type questions] 4 x 10 = 40 Marks
------------------------ Total 80 Marks ------------------------
The passing minimum for this paper is 40%In case, the candidate fails to secure 40% passing minimum, he / she may have to reappear for the same in the subsequent semesters7. Guidelines for General Awareness (Part IV)
Components Marks
Two Tests (2 x 50) 100
The question paper pattern is as follows:Test I – 2 hours [50multiple choice questions] 50 x 1 = 50Marks Test II – 2 hours [50 multiple choice questions] 50 x 1 = 50 Marks
------------------------- Total 100 Marks -------------------------
The passing minimum for this paper is 40%In case, the candidate fails to secure 40% passing minimum, he / she may have to reappear for the same in the subsequent semesters
8. Guidelines for Law of Ethics (Part V)
Components MarksTwo Tests (2 x 50) 100
The question paper pattern is as follows:Test I – 2 hours [5 out of 8 essay type questions] 5 x 10 = 50Marks Test II – 2 hours [5 out of 8 essay type questions] 5 x 10 = 50 Marks
-------------------------- Total 100 Marks --------------------------
The passing minimum for this paper is 40%In case, the candidate fails to secure 40% passing minimum, he / she may have to reappear for the same in the subsequent semesters
9. Guidelines for Extension Activity (Part V)
BOS meeting approved: Approved in 5thAcademic Council meeting on :
At least two activities should be conducted within this semester (IV) consisting of two days each. The activities may be Educating Rural Children, Unemployed Graduates, Self Help Group etc.
The marks may be awarded as follows
No of Activities Marks
2 x 50( Each Activity for two days)
100
10. QUESTION PAPER PATTERN FOR EE (Part III Theory Papers)
Duration: Three Hours Maximum: 70 Marks
SECTION - A (10x1=10 Marks)Answer ALL Questions
ALL Questions Carry EQUAL Marks
Q.No 1 to 10: (Multiple choice/Fill up the blanks /True or False questions). (Two questions from each unit)
SECTION - B (5x4=20 Marks)Answer ALL Question
ALL Questions Carry EQUAL Marks
Q.No 11 to 15: Either or type questions (One question from each Unit)
SECTION- C (5x8=40 Marks)Answer ALL Questions
ALL Questions carry EQUAL Marks
Q.No 16 to 20: Either or type questions (One question from each Unit)
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: DSC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU01 Course Title Batch: 2019-2022CELL BIOLOGY AND GENETICS Semester: I
Hrs/Week: 5 Credits: 5
Course Objective1.To enable the students to learn the basics of Cell Biology and Genetics2.Strong foundation in understanding the composition of cells and to know in detail the various fields of Genetics
Course Outcomes (CO)
K1 CO1 The knowledge of cell structures, functions, cellular organelles and various metabolic processes will help students to design experiments with appropriate understanding and leads to conduct genuine research.
K2 CO2 It will make students able to think critically in reading and analyzing biological information and will demonstrate the activity to articulate, verbally and in writing knowledge of biology, biological methods and biological issues in context.
K3 CO3 The students will gain knowledge about the inheritance pattern of the genes and genetic diseases and also about the gene frequencies in the population.
K4 CO4 The students can take up career in research or clinical molecular genetics labs .
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S S S SCO2 S M S SCO3 S S S MCO4 M S S S
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU01 CELL BIOLOGY AND GENETICS I
Unit No. Topics Hours
Unit I
Cellular OrganizationConcepts of Cell Theory --Cell as a basic unit of life- classification of cell types (bacteria, fungi, virus, plant and animals) - Prokaryotic and Eukaryotic cell Organization. Cell Organelles: Structure and Function of Plasma Membrane, Chloroplast, Endoplasmic Reticulum, Golgi Apparatus, Nucleus, Ribosomes, Mitochondria and Cell Inclusions – lysosomes, Peroxysomes, gas vacuoles
12
.
Unit II
Cell Cycle, Cell Division and ChromosomesCell Growth – Cell Cycle – Cell Divisions –Mitosis and Meiosis significance– Chromosome - Organization in prokaryotes, Eukaryotes, and virus, Structure and function of specialized chromosomes (Polytene, and Lampbrush )
12
Unit III
Mendelian GeneticsHistory of Genetics - Mendelian Principles, Segregation, Independent Assortment, Dominance. Multiple alleles, Pseudo alleles, Incomplete dominance, Over dominance and co dominance, complementation test.Non -Mendelian GeneticsChromosomal theory of inheritance, maternal effects.
12
Unit IVCytogenetics Linkage, crossing over, Mechanism, Types of crossing over (Single, Double and Multiple) gene mapping, Chromosomal Mutations – Numerical changes-Anuploidy,Eupoidy-Structural changes- Insertion - Deletion, Duplication, Inversion, Translocation
12
Unit V
Population GeneticsGene Pool and Gene Frequency- Two Models of Gene pool – Chance Mating or Panmixis - Hardy Weinberg’s Law – Genetic Drift –– speciation and evolution Eugenics, Pedigree Analysis
12
Text Book:1.Verma P.S and Agarwal V.K, “Genetics” , S Chand and Sons, NewDelhi.2.Verma P.S and Agarwal V.KCell Biology, Genetics, Molecular Biology, Evolution and Ecology S Chand and Sons, NewDelhiReference Books:1.Molecular cell Biology, by Darnell, Lodish, Baltimore, Scientific American Books, Inc., 1994. 2. Molecular and cellular Biology, Stephen L.Wolfe, Wadsworth Publishing Company, 1993. 3. Molecular Cloning: a Laboratory Manual, J. Sambrook, E.F. Fritsch and T. Maniatis, Cold Spring Harbor Laboratory Press, New York, 2000. 4.The science of Genetics by Alan G. Atherly, Jack. R, Girton, Jhon. F, Mc Donald. Sounders college publishers. 5. Genes VII by Benjamin Lewin
Course Designed by Verified by HOD Checked by Approved by
DR.K.MANIMEKALAI
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: GE Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU02 Course Title Batch: 2019-2022Allied I - CHEMISTRY (BIO) Semester: I
Hrs/Week: 5 Credits: 3
Course ObjectiveTo Make the Student Acquire Sound Knowledge With the Principles of Chemical Bonding, Stereoisomerism, Inter-Halogen Compounds, Chemistry of Heterocyclic Compounds, Dye Chemistry and Chemical Kinetics.
Course Outcomes (CO)
K1 CO1 Acquire foundations in the fundamentals and application of current chemical and scientific theories.
K2 CO2 Students will be able to design and carry out scientific experiments precisely and analyze the results accurately.
K3 CO3 Explain the behavior of, and interactions between, matter and energy at both the atomic and molecular levels.
K4 CO4 Understand the Electrophilic substitution reactions including identifying the oxidation, reduction, oxidation agent and reducing agent..
K5 CO5 Learn effective steps used to learn the preparation of chemicals for bioanalytical studies.
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S S M LCO2 S M M MCO3 S L M MCO4 S M M L
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU02 Allied I - CHEMISTRY (BIO) I
Unit No. Topics Hours
Unit I
Chemical bondingMolecular Orbital Theory - MO diagram of H2, He2, N2 and O2 Molecules. Interhalogen Compounds – Preparation, Properties, Structure and uses of ICl, BrF3. Diborane – Preparation, Properties and Structure.
12
Unit II
Hybridization and StereoisomerismOrbital Overlap - Geometry of Organic Molecules – Methane, Ethylene and Acetylene. Inductive Effect, Electromeric Effect, Mesomeric Effect - Effects in Properties of Compounds. Stereoisomerism - Optical Isomerism, Symmetry - Elements of Symmetry, Cause of Optical Activity - Optical Activity in Lactic and Tartaric acid. Racemisation and Resolution - Geometric Isomerism exhibited by Maleic and Fumaricacid.
12
Unit III
Chemical KineticsIntroduction - Rate of a Reaction - Order and Molecularity of aReaction– Examples. Various Order of a Reaction – Zero, First, Second Order Reactions - Pseudo First Order Reaction - Methods of Determining Order of a Reaction - Effect of Temperature on Reaction Rate – Energy of Activation.
12
Unit IV
Aromatic compoundsElectrophilic substitution in benzene - Mechanism of nitration, halogenation, alkylation, acylation, sulphonation - Preparation and properties of naphthalene. Heterocyclics: Preparation and properties of furan and pyrrole.
Text Book :1.Veeraiyan V. and Vasudevan A.N.S, “Allied Chemistry Paper I & II”, High Mount Publishing House, Chennai.Reference Books:1. Puri B.R., Sharma L.R and Pathania M.S, “Principles of Physical Chemistry”, Vishal Publishing
Co.,NewDelhi.2. Puri B.R and Sharma L.R, “Inorganic chemistry”, Shobanlal Nagin Chand and Co., NewDelhi.
Course Designed by Verified by HOD Checked by Approved by
Mrs.R.GOHILA
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: SEC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU03Course Title Batch: 2019-2022
PRACTICAL I -CELL BIOLOGY AND GENETICS Semester: I
Hrs/Week: 4 Credits: 3
Course Objective1. To enable the students to understand the basic techniques in Cell Biology, Genetics2. Strong foundation in understanding the composition of cells and to know in detail the various fields of Genetics
Course Outcomes (CO)
K1 CO1 Students should be able to demonstrate an understanding of the characteristics of living organisms including their chemical composition, cellular structure, and cellular metabolism
K2 CO2 Students should be able to apply methods of scientific measurement, analyze experimental data and report experimental results in scientific format
K3 CO3 Students should be able to demonstrate critical thinking skills.
K4 CO4 Students should be able to demonstrate an understanding of the Chemical Hazards Communication Standard and how it applies to a laboratory setting
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S S M LCO2 S M L MCO3 S S L MCO4 S M M L
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU03 PRACTICAL I -CELL BIOLOGY AND GENETICS
I
Unit No. Topics Hours
Cell Biology1. Laboratory Rules and Regulations.2. Microscopy – Light Microscope – Compound Microscope – Dark – Field Microscope and Bright Field Microscope.3. Identification of, Bacteria, Fungi, Animal and Plant cells4. Identification of Lymphocytes.5. Fraction of Cellular components - Chloroplast and Mitochondria.6. Mitotic Preparation - Onion Root Tip.7. Meiosis 8. Cell Permeability9. Karyotyping- Demo
Genetics10.Drosophila – Morphology, Section Culture and Maintenance.11.Identification of Mutant s - Physical and Chemical Methods.12.Monohybrid and Dihybrid Cross Using Plants - Demo13.Identification of Barr Bodies.14.Mounting of Polytene Chromosome from Chironomous Larvae.
Reference Books:1. Molecular Cloning: a Laboratory Manual, J. Sambrook, E.F. Fritsch and T. Maniatis, Cold pring
arbor Laboratory Press, New York, 2000.2. Molecular and cellular Biology, Stephen L.Wolfe, Wadsworth Publishing Company, 1993. 3. Introduction to Practical Molecular Biology, P.D.Dabre, John Wiley & Sons Ltd., New York,
1998. 4. Human genetics, A.Gardner, R.T.Howell and T.Davies, Published by VinodVasishtha for Viva
Books private limited, 2008.5. Molecular Biology of the Gene (4th Edition), J.D.Watson, N.H.Hopkins, J.W.Roberts, J.A.
Steitz and A.M.Weiner, The Benjamin/Cummings Publ. Co., Inc., California, 1987.
Course Designed by Verified by HOD Checked by Approved by
DR.K.MANIMEKALAI
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: SEC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU04Course Title Batch: 2019-2022
ALLIED PRACTICAL I -LAB IN CHEMISTRY Semester: I
Hrs/Week: 4 Credits: 2
Course ObjectiveTo enable the student to understand the basics of Acid- Base Titrations and to analyze the
Organic Substance systematically and to study the Concepts and Working Principle of Laboratory Equipments
Course Outcomes (CO)
K1 CO1 To understand about volumetric analysis of various acids and bases.
K2 CO2 To understand about organic analysis of aliphatic, aromatic, saturated and unsaturated elements.
K3 CO3 To understand about working principle and functions of laboratory equipmentsK4 CO4 To understand about various chromatographic analysis
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S S M LCO2 S M S LCO3 S L M MCO4 S M L M
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU04 ALLIED PRACTICAL I -CHEMISTRY (BIO)
I
Unit No. Topics Hours
ChemistryVolumetric Analysis
1. Estimation of Hydrochloric Acid- Standard OxalicAcid.
2. Estimation of Ferrous Sulphate- Standard Mohr SaltSolution.
3. Estimation of Potassium Permanganate - Standard SodiumHydroxide
Organic Analysis
4. Detection of Elements ( N, S,Halogens).
5. To distinguish between Aliphatic and Aromatic.
6. To distinguish between Saturated and Unsaturated.
7. Functional group tests for Phenols, Dicarboxylic Acids, Aromatic
8. Primary Amine, Diamide, Carbohydrate.
9. Functional groups characterized by Confirmatory Test
Reference Books:1. Jesse E. Day ,Practical Chemistry with Experiments. Revised edition,(Newell, Lyman C.), J. Chem.
company, New Delhi.4.D.H.Williams-Ian Fleming, Spectroscopic Methods in Organic Chemistry,Mc Graw HillPublishing Company Ltd, 2006.
Course Designed by Verified by HOD Checked by Approved by
MRS.R.GOHILA
Programme Code: DSC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Course Code: 19BTU05 Course Title Batch: 2019-2022
BIOCHEMISTRY Semester: IIHrs/Week: 5 Credits: 5
Course Objective1. To Enable the Students to Understand the Basic Concepts in Biological Chemistry with the
Emphasis on Biomolecules, its Metabolism and Function in Living System.2. On the successful completion of the course the students will get an overallunderstanding of the
structure and functions of biomolecules, enzyme kinetics, biopolymers and metabolic reactions in a living system.
Course Outcomes (CO)
K1 CO1 This paper in biochemistry has been designed to provide the student with a firm foundationin the biochemical aspects of cellular functions which forms a base for their future research.
K2 CO2 Students can able to recognize the structures and functions of biomolecules that formthe basis of what we understand to be living organisms.
K3 CO3 By the end of the course students could able to understand the principles of biochemicalpathways which regulate the cellular mechanisms.
K4 CO4 Further, the students will be exposed to the limitations of biomolecules in regulation ofmolecular functions in mammals especially in humans.
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S M M LCO2 S S M LCO3 S S L LCO4 S S L M
S - Strong; M-Medium; L-Low.
19BTU05 BIOCHEMISTRY I
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Unit No. Topics Hours
Unit I
Structure of Atoms and BiomoleculesAtomic theory – Valency - Atomic weight - Molecular weight - Molarity. Chemical Bonding – Electrostatic – Covalent - Ionic and Vander Waal’s. Structure of Water Molecules - Properties and Ionization of Water. pH and Buffers.
12
Unit II
Carbohydrates and ProteinsCarbohydrates: Classification, Structure and Functions - Physical and Chemical Properties. Proteins: Amino Acids and Peptides - Structure and Classification of Amino Acids - Classification and Organization of Proteins – Primary – Secondary - Tertiary and Quaternary - BiologicalSignificance.
12
Unit IIIEnzymes and Nucleic AcidIUB Classification and Nomenclature of Enzymes - Michaelis-Menten Equation - Active Sites - Activators and Inhibitors. Isoenzymes -Allosteric Enzymes. Nucleic Acids: Structure - Properties and Functions of Nucleic Acids – DNA - RNA and their Types.
12
Unit IVLipids and VitaminsClassification - Structure and Functions. Triglycerides – Phospholipids - Glycolipids and Lipoproteins - Structure and Function. Vitamins: Types- Structure and Functions.
12
Unit V
MetabolismGlycolysis - TCA Cycle - Glycogen Breakdown and Synthesis - Gluconeogenesis - Electron Transport Chain and Oxidative Phosphorylation – Photophosphorylation - Biosynthesis and Degradation of Nucleic Acids (Purines and Pyrimidines).
12
Text Book:1. Principles of Biochemistry – Smith et al., McGraw – Hill International book Company,8th Edition.2. Principles of Biochemistry – Lehninger , Nelson, Cox, CBS publishersReference Books:1. Fundamentals of Biochemistry – Voet et al., John Wiley and Sons, Inc.2. Biochemistry – Zubay , WCB publishers3. Harper‟s Biochemistry – R.K.Murray, D.K.Granner, P.A.Mayes and V.W Rodwell,Prentice-Hall International.4. Biochemistry (VIthEd.) – J.M Berg; J.L.Tymoczko and L.Stryer, W H Freeman andCompany, NY.5.Biochemistry (3rd Edition) - Christopher K. Mathews, Kensal E. van Holde, Kevin G.Ahern, Pearson Education.
Course Designed by Verified by HOD Checked by Approved by
Dr.R.MOHAMMED RAFIQKHAN
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: GE Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU06 Course Title Batch: 2019-2022
Course Objective1. To establish the substantial foundation in Bioanalytical Techniques of students demanded for
the field of Instrumentation in life sciences.2. To gear up student to lend oneself reasoning inferred by the contextual knowledge to
bioanalytical practices, biological phenomena using physical laws in understanding principles and working mechanism of analytical equipment.
Course Outcomes (CO)
K1 CO1 Students can envisage themselves toexplain the core concepts on inter and intramolecular interactions, structures of bio-molecules, acids, bases and buffers.
K2 CO2 Students will get them familiarize and able to perform and analyze various analytical equipment, techniques and applications in Clinical, Environmental, Food & Beverage.
K3 CO3 Students should be able to interpret and critically analyze various spectroscopies and their principles and applications in environmental and biomedical fields
K4 CO4 Students will have a thorough knowledge on various chromatography, and electrophoresis techniques and their applications in biomedical sciences
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S M L MCO2 S S L LCO3 S M M MCO4 S S L L
S - Strong; M-Medium; L-Low
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU06 BIOANALYTICAL TECHNIQUES I
Unit No. Topics Hours
Unit I
Acid, Bases and Buffers: Ionization, dissociation, acidity, basicity, theories of acid and bases, strength of acids and bases, acid-base equilibrium in aqueous and non-aqueous media. pH-dependent functions and structures of bio-molecules, Henderson–Hasselbach equation, use of indicators, buffers, amino acid titrations, biological buffers.
10
Unit II.Centrifugation: Principles of sedimentation, relation between g and rpm, instrumentation, working and applications of preparative and analytical ultracentrifugation, isopycnic centrifugation, rate zonal centrifugation
10
Unit III
Spectroscopic Techniques: Beer-Lambert’s law and its verifications and applications, scattering, phosphorescence, fluorescence, luminescence, diffraction spectra, Application of UV-Visible and FTIR spectroscopy, turbidometry and nephlometry.
10
Unit IVChromatography: Principles, instrumentation, working and applications of paper partition chromatography, TLC, HPTLC, Affinity, Gel filtration, ion exchange, hydrophobic interaction andreverse phase (HPLC/FPLC) chromatography and GC.
9
Unit V
Electrophoresis: Principles, factors affecting electrophoresis, support media used, instrumentation, denaturing/ non-denaturing/ non-reducing DNA and protein gel electrophoresis, working and applications of electrophoretic techniques-zone, disc, capillary, 2-D, pulsed-field gel electrophoresis, diagonal, isoelectric focusing, immune electrophoresis
9
Text Book:1.Veerakumari. L, “Bioinstrumentation”, MJP Publishers, Chennai.2. Abhilasha Shourie., Shilpa S. Chapadgaonkar., Bioanalytical Techniques.The Energy and Resources Institute, TERIReference Books:1.Sawhney S. K. and RandhirSingh, “Introductory to Practical Biochemistry”, Narosa Publishing House, New Delhi.2. Boyer Rodney F, “Modern Experimental Biochemistry”, Benjamin Cummings Publication, Sydney.3. Fundamentals of Bioanalytical Techniques and Instrumentation. Sabari Ghosal, Anupama Sharma Awasthi., PHI Learning; Second edition4. Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology.Andreas Hofmann, Samuel Clokie.Cambridge University Press; 8 edition5. Instrumental Methods in Bioanalytical Chemistry Paperback – 1 Jan 2016.S M Khopkar.New Age International Private Limited
Course Designed by Verified by HOD Checked by Approved by
DR. S.G. ANTONY GODSON
BOS meeting approved: Approved in 5thAcademic Council meeting on :
BACHELOR OF SCIENCE
Programme Code: SEC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU07Course Title Batch: 2019-2022
PRACTICAL III -BIOCHEMISTRY Semester: I
Hrs/Week: 4 Credits: 2
Course Objective1.To enable the students to learn the basic functions, structures and biologicalimportance of lifeless chemical compounds.2.It helps the biochemistry students in understanding the basic science in a variety ofapplications.
Course Outcomes (CO)
K1 CO1 To learn the chromatographic techniques for the separation of the individualcompound from the mixture of compound.
K2 CO2 To study the interaction between matter and electromagnetic radiation and visiblelight dispersed according to its wavelength, by a prism.
K3 CO3 To understand the characterization of surfaces using radioisotopes generallyinvolves observing the manner in which the radioactive species interact with thesurface.
K4 CO4 To obtain knowledge about the separation and analysis of macromolecules andtheir fragments, based on their size and charge
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S M M MCO2 S S L LCO3 S S M MCO4 S S L M
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU07 PRACTICAL II- BIOCHEMISTRY I
Unit No. Topics Hours
Biochemistry 1. Estimation of Protein by Lowry’s method. 2. Estimation of DNA by DPA Method. 3. Estimation of RNA by Orcinol method.4. Estimation of Sugars by Anthrone method. 5. Estimation of amino acids by Ninhydrin method. 6. Analysis of Oils- Iodine Number - Saponification Value - Acid Number.7. Quantification of Vitamin C.8. Estimation of inorganic phosphate by Subbarow method 9. Qualitative tests of Sugars, amino acids and lipids10. To study the effect of pH, temperature on the activity of salivary amylase
enzyme
Reference Books:
1. Experimental Biochemistry: A Student companion- Sashidhar Rao, B and Deshpande,V. IK International (P) Ltd.
2. Experiments and Techniques in Biochemistry: by Sheel Sharma, Galgotia publications.3. Instrumental methods of chemical analysis – P.K. Sharma.4. Biophysical chemistry – Upadhyay., Upadhyay and Nath.5. Handbook of Biomedical Instrumentation – R.S. Khandpur, Tata McGraw Hill.
Course Designed by Verified by HOD Checked by Approved by
Dr.K.MOHAMMED RAFIQKHAN
BOS meeting approved: Approved in 5thAcademic Council meeting on :
Programme Code: SEC Programme Title: Bachelor of SCIENCE IN BIOTECHNOLOGY
Course Code: 19BTU08 Course Title Batch: 2019-2022ALLIED PRACTICAL II -
BIOANALYTICAL TECHNIQUES (BIO)
Semester: II
Hrs/Week: 03 Credits: 02
Course Objective1. To enable the student to understand the basics of different analytical concepts, techniques and their
working Principle and operating procedure.2. To provide scientific understanding of analytical techniques, equipments and detail interpretation of results
Course Outcomes (CO)
K1 CO1 Students can handle and demonstrate experiments and critically analyzevariousLaboratory Equipment: Laminar Air Flow,Air Oven, Weighing balance, and preparation of buffers
K2 CO2 Students should be able to demonstrate an understanding of the characteristics of principle of Principles of Colorimetry: Beer's law
K3 CO3 Students will be able to demonstrate various separation techniques, identification, and quantification
K4 CO4 Students should be able to equip themselves with contemporary sophisticated equipment and able to elucidate and characterize their essential features and principle
Mapping of Outcomes
POCO PO1 PO2 PO3 PO4
CO1 S S M LCO2 S M L MCO3 S M S LCO4 M S S M
S - Strong; M-Medium; L-Low.
BOS meeting approved: Approved in 5thAcademic Council meeting on :
19BTU08 ALLIED PRACTICAL II -BIOANALYTICAL TECHNIQUES (BIO)
I
Unit No. Topics Hours
1. Working principle of Laboratory Equipment: Autoclave, Incubator,
Laminar Air Flow, Hot Air Oven, Weighingbalance.
2.Preparation of Buffers (pH)
3. Determination of pH using pH meter.
4. Principles of Colorimetry: (i) Beer's law, estimation of protein. (ii)
To study relation between absorbance and % transmission
5. Separation of Amino acids by paper chromatography.
6.Thin Layer chromatography
7.Agarose Gel Electrophoresis
8.Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis
(SDSPAGE)
9.Demonstration: HPLC
10.Demonstration: GC-MS
11.Demonstration: LC-MS
Text Book:1.Immunoassay and Other Bioanalytical Techniques by Jeanette M. van Emon.CRC Press2. Fundamentals of Bioanalytical Techniques and Instrumentation.,A.K. Shrivastava,Sabari Ghosal.PHI Learning, 2009Reference Books:1. Fundamentals and Techniques of Biophysics and Molecular Biology.Pranav Kumar.Pathfinder Publication, a unit of Pathfinder Academy Pvt. Ltd; Updated edition (2016)2. Boyer Rodney F, “Modern Experimental Biochemistry”, Benjamin Cummings Publication,Sydney.3. Practical HPLC Method Development by by Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch.Wiley India Pvt Ltd; Second edition4. Hans-Joachim Hübschmann: Handbook of GC-MS: fundamentals and applications, 3rd ed. By Markus R. Meyer. Springer-Verlag Berlin Heidelberg 20165. The Essence of Chromatography by C. F. Poole.Elsevier, 2003.
.Course Designed by Verified by HOD Checked by Approved by
DR. S.G. ANTONY GODSON
BOS meeting approved: Approved in 5thAcademic Council meeting on :
