Citation: Gurdev S Khush, S.S. Virmani and D.S. Brar. 1990. Expectations of plant breeders from tissue culture. In S.S. Bhojwani (Ed.) Plant Tissue Culture: Applications and Limitations. Elsevier Amsterdam - Oxford - New York - Tokyo. pp. 412-423.

Keywords: Oryza sativa, plant breeding, major plant breeding objectives, transfer of alien genes, diversification of cytoplasmic male sterility, product of hybrids and organelle recombinants, induction of somaclonal variation, enhancing introgression of genes, transgenic crop varieties, reducing breeding cycle, exercising marker-based selection, characterization of pathogens, in-vitro selection at cellular level, large scale multiplication of elite germplasm,