Supporting Information

Altered learning, memory and social behavior in type 1 taste receptor subunit 3 knockout mice is associated with neuronal

dysfunction

Bronwen Martin1*, Rui Wang1*, Wei-Na Cong1, Caitlin M. Daimon1, Wells W. Wu1, Bin Ni2, Kevin G. Becker3, Elin Lehrmann3, William H. Wood III3, Yongqing Zhang3, Harmonie

Etienne4,5, Jaana van Gastel4,5, Abdelkrim Azmi4,5, Jonathan Janssens4,5, Stuart Maudsley2,4,5†

From the 1Metabolism Unit, National Institute on Aging, National Institutes of Health, Baltimore, MD. USA, 2Receptor Pharmacology Unit, National Institute on Aging, National Institutes of Health, Baltimore, MD. USA, 3Gene Expression and Genomics Unit, National Institute on Aging, National Institutes of Health, Baltimore, MD. USA, 4Translational Neurobiology Group, VIB Department of Molecular Genetics, University of Antwerp, Antwerp, Belgium, 5Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium

Running title: T1R3 KO mice have altered cognitive function

* These authors contributed equally.

† To whom correspondence should be addressed: Dr. Stuart Maudsley, University of Antwerp, VIB Department of Molecular Genetics, Universiteitsplein 1, Antwerp 2610; Telephone: +32 3 265 1057; FAX: +32 3 265 1113; E-mail: Stuart.Maudsley@molgen.vib-ua.be

Keywords: Dendritic, spine, hippocampus, cognition, learning, sociability, neurotrophic

SUPPLEMENTAL INFORMATION - CONTENTS

FIGURE S1. Comprehensive activity evaluation of T1R3KO mice compared to WT controls.

TABLE S1. Gene transcripts significantly and differentially regulated in T1R3KO cortex compared to WT control cortex.

TABLE S2. Gene transcripts significantly and differentially regulated in T1R3KO hippocampus compared to WT control cortex.

TABLE S3. Gene transcripts significantly and differentially regulated in T1R3KO hypothalamus compared to WT control cortex.

TABLE S4. Venn diagram-based dissection of T1R3KO-specific transcriptomic expression patterns between cortical, hippocampal and hypothalamic tissues.

TABLE S5. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO cortex compared to WT controls.

TABLE S6. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO hippocampus compared to WT controls.

TABLE S7. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO hypothalamus compared to WT controls.

TABLE S8. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO cortex compared to WT controls.

TABLE S9. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO hippocampus compared to WT controls.

TABLE S10. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO hypothalamus compared to WT controls.

TABLE S11. Proteins altered in expression between T1R3KO and WT hippocampal tissues.

TABLE S12. Canonical signaling pathway analysis of proteins significantly and differentially regulated in T1R3KO mice hippocampus compared to WT control mouse hippocampus.

TABLE S13. BioFunction/Disease pathway analysis of proteins significantly and differentially regulated in T1R3KO mice hippocampus compared to WT control mouse hippocampus.

TABLE S14. Proteins altered in expression between T1R3KO and WT cortical tissues.

TABLE S15. Canonical signaling pathway analysis of proteins significantly and differentially regulated in T1R3KO mice cortex compared to WT control mouse cortex.

TABLE S16. BioFunction/Disease pathway analysis of proteins significantly and differentially regulated in T1R3KO mice cortex compared to WT control mouse cortex.

TABLE S17. Textrous!-based protein clustering of significantly-regulated proteins from T1R3KO hippocampus versus WT control hippocampus.

TABLE S18. Textrous!-based protein clustering of significantly-regulated proteins from T1R3KO cortex versus WT control cortex.

TABLE S19. Textrous!-based extracted noun-phrases from differential hippocampal T1R3KO versus WT control protein expression data.

TABLE S20. Textrous!-based extracted noun-phrases from differential cortical T1R3KO versus WT control protein expression data.

TABLE S21. WriteWords-generated word frequency scores from Textrous!-extracted noun-phrases from differential protein expression data from T1R3KO hippocampus.

TABLE S22. WriteWords-generated word frequency scores from Textrous!-extracted noun-phrases from differential protein expression data from T1R3KO cortex.

FIGURE S1. Comprehensive activity evaluation of T1R3KO mice compared to WT controls. The activity status of T1R3KO mice compared to WT controls was assesse using a CLAMS system across a variety of outputs (WT, black triangles; T1R3KO, white squares), including (A) X-axis total activity, (B) X-axis ambulatory activity, (C) Z-axis total activity, (D) Accumulated feeding and (E) Accumulated water intake. Parameters in all panels were measured over a 48-h period. The dark cycle time is indicated by the grey section of the pictogram bar in each CLAMS activity. Data are means ± S.E.M, p ≤ 0.05 was considered statistically significant, n = 8.

Table S1. Gene transcripts significantly and differentially regulated in T1R3KO cortex compared to WT control cortex. For each significantly-regulated transcript the Official Gene Symbol, transcript description and expression Z ratio, T1R3KO versus WT are represented.

Gene Symbol Description Z ratio T1R3KO:WTBC032265 cDNA sequence BC032265 10.89Tmem87a transmembrane protein 87A 9.14Stbd1 starch binding domain 1 7.47Cap1 CAP, adenylate cyclase-associated protein 1 6.84Fcgr3 Fc receptor, IgG, low affinity III 6.68Hpcal1 hippocalcin-like 1 6.64Rgs5 regulator of G-protein signaling 5 6.25Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog 6.13Ndufb10 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 6.01Smpdl3b sphingomyelin phosphodiesterase, acid-like 3B 5.71Nubp2 nucleotide binding protein 2 5.542310002B06Rik RIKEN cDNA 2310002B06 gene 5.41Trim2 tripartite motif protein 2 5.2Trhr thyrotropin releasing hormone receptor 5.2Col9a2 collagen, type IX, alpha 2 5.12Hyi hydroxypyruvate isomerase homolog (E. coli) 5.11Sfrp1 Secreted frizzled-related protein 1 4.81Itgb3bp integrin beta 3 binding protein (beta3-endonexin) 4.7EG245297 predicted gene, EG245297 4.61Camk2d calcium/calmodulin-dependent protein kinase II, delta 4.59Rbm45 RNA binding motif protein 45 4.44Cox18 COX18 cytochrome c oxidase assembly homolog (S. cerevisiae) 4.41Wars2 tryptophanyl tRNA synthetase 2 (mitochondrial), nuclear gene encoding mitochondrial protein 4.4Marcksl1 MARCKS-like 1 4.31110017D15Rik RIKEN cDNA 1110017D15 gene 4.24Wfs1 Wolfram syndrome 1 homolog (human) 4.19Nubp2 nucleotide binding protein 2 4.19Epha3 Eph receptor A3 4.16Alad aminolevulinate, delta-, dehydratase 4.13Ly6g6e lymphocyte antigen 6 complex, locus G6E 4.02Tmem91 transmembrane protein 91 4Dcn decorin 3.97Gnb1 guanine nucleotide binding protein (G protein), beta 1 3.93Tmem132e transmembrane protein 132E 3.91Lpin2 Phosphatidate phosphatase LPIN2 3.87Hmgn2 high mobility group nucleosomal binding domain 2 3.81Apaf1 apoptotic peptidase activating factor 1 3.76Pgam2 phosphoglycerate mutase 2 3.72Clec16a C-type lectin domain family 16, member A 3.7Dcn decorin 3.7Vars2 valyl-tRNA synthetase 2, mitochondrial 3.66Ntng1 netrin G1 3.63Adamts4 A disintegrin and metalloproteinase with thrombospondin motifs 4 3.59Inpp4b inositol polyphosphate-4-phosphatase, type II 3.58Dnaic1 dynein, axonemal, intermediate chain 1 3.53Ttl tubulin tyrosine ligase 3.52BC048546 cDNA sequence BC048546 3.5

Nrsn2 neurensin 2 3.48BC048546 cDNA sequence BC048546 3.48Sez6 seizure related gene 6 3.43Tnnt1 troponin T1, skeletal, slow 3.41Moxd1 monooxygenase, DBH-like 1 3.41Gpr19 G protein-coupled receptor 19 3.34Snapc3 small nuclear RNA activating complex, polypeptide 3 3.31Fibcd1 fibrinogen C domain containing 1 3.23Scp2 sterol carrier protein 2, liver 3.19Wnt7b wingless-related MMTV integration site 7B 3.11C4b complement component 4B 3.1Tbc1d8 TBC1 domain family, member 8 3.09Hn1 hematological and neurological expressed sequence 1 3.06Cacna1h calcium channel, voltage-dependent, T type, alpha 1H subunit 2.99Odz3 odd Oz/ten-m homolog 3 (Drosophila) 2.99Gpr83 G protein-coupled receptor 83 2.98Pnck pregnancy upregulated non-ubiquitously expressed CaM kinase 2.96Ddo D-aspartate oxidase 2.96Ntng1 netrin G1 2.95Sdhc succinate dehydrogenase complex, subunit C, integral membrane protein 2.94Gins4 GINS complex subunit 4 2.94Cmbl carboxymethylenebutenolidase-like (Pseudomonas) 2.86Ttc39a tetratricopeptide repeat domain 39A 2.84LOC100046744 similar to Serine/arginine repetitive matrix protein 2 2.84Sap130 Sin3A associated protein 2.83Extl1 exostoses (multiple)-like 1 2.82Top1mt DNA topoisomerase 1, mitochondrial 2.81Cpne6 copine VI 2.8Fahd2a fumarylacetoacetate hydrolase domain containing 2A 2.76Chga chromogranin A 2.76Grb7 growth factor receptor bound protein 7 2.74Mrps10 mitochondrial ribosomal protein S10 2.71Cachd1 cache domain containing 1 2.71Cdkal1 threonylcarbamoyladenosine tRNA methylthiotransferase 2.67Cdh13 cadherin 13 2.64Syt9 synaptotagmin IX 2.63Gfer growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration) 2.63Mcm6 minichromosome maintenance deficient 6 2.62Cpne2 copine II 2.62Hdhd3 haloacid dehalogenase-like hydrolase domain containing 3 2.6Pex6 peroxisomal biogenesis factor 6 2.6Mrps15 mitochondrial ribosomal protein S15 2.57Crtac1 Cartilage acidic protein 1 2.57Col4a2 collagen, type IV, alpha 2 2.57Mettl1 methyltransferase-like 1 2.54Dlx2 distal-less homeobox 2 2.53Tmem35 transmembrane protein 35 2.51Phactr4 phosphatase and actin regulator 4 2.49Pdyn prodynorphin 2.48Ddo D-aspartate oxidase 2.48Tnfaip8 tumor necrosis factor, alpha-induced protein 8 2.47Slc29a1 solute carrier family 29 (nucleoside transporters), member 1 2.46C2cd4c C2 calcium-dependent domain containing 4C 2.46Tiam1 T-cell lymphoma invasion and metastasis 1 2.44

Creb3 cAMP responsive element binding protein 3 2.44Dpysl3 dihydropyrimidinase-like 3 2.41Asah3l N-acylsphingosine amidohydrolase 3-like 2.41Gnai2 guanine nucleotide binding protein, alpha inhibiting 2 2.41Emid2 EMI domain containing 2 2.4Habp4 hyaluronic acid binding protein 4 2.4Gng10 guanine nucleotide binding protein 2.4Usp11 ubiquitin specific peptidase 11 2.39Lrrc33 leucine rich repeat containing 33 2.39Kirrel3 kin of IRRE like 3 2.38Rgs12 regulator of G-protein signaling 12 2.38Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase 2.38Ssbp3 single-stranded DNA binding protein 3 2.37Gfap glial fibrillary acidic protein 2.36LOC677317 similar to Mod1 protein, transcript variant 4 2.35Cox18 COX18 cytochrome c oxidase assembly homolog 2.35Lamc1 laminin, gamma 1 2.34Lin7b lin-7 homolog B 2.34Pex6 peroxisomal biogenesis factor 6 2.31Coq5 coenzyme Q5 homolog, methyltransferase 2.29Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase 2.29Nol4 nucleolar protein 4 2.28Chga chromogranin A 2.27Sumf2 sulfatase modifying factor 2 2.25Acy1 aminoacylase 1 2.25H1fx H1 histone family, member X 2.24Ccrk cell cycle related kinase 2.23Reln reelin 2.23Rsph1 radial spoke head 1 homolog 2.23Slc25a1 solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1 2.22Lrrc1 leucine rich repeat containing 1 2.22Rgs17 Regulator of G-protein signaling 17 2.22Palm paralemmin 2.22Dapk1 death associated protein kinase 1 2.21Syngr3 synaptogyrin 3 2.21 March 8 membrane-associated ring finger (C3HC4) 8 2.21Tspo translocator protein 2.2Dgkg diacylglycerol kinase, gamma 2.2Wnt2 wingless-related MMTV integration site 2 2.2Tmem150 transmembrane protein 150 2.19Igsf9 immunoglobulin superfamily, member 9 2.18Tmem176b transmembrane protein 176B 2.18Taf10 TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor 2.18Ndufaf5 NADH dehydrogenase 2.17Mlp MARCKS-like 1 2.16Lgals1 Galectin-1 2.15Ntsr1 neurotensin receptor 1 2.15Ass1 argininosuccinate synthetase 1 2.14Hexim2 hexamthylene bis-acetamide inducible 2 2.13Gas2l1 growth arrest-specific 2 like 1 2.13Casc4 cancer susceptibility candidate 4 2.13Pacrg Park2 co-regulated 2.12D4Ertd22e DNA segment, Chr 4, ERATO Doi 22, expressed 2.1Lbh limb-bud and heart 2.1

Rnaseh2c ribonuclease H2, subunit C 2.09Lrrfip1 leucine rich repeat (in FLII) interacting protein 1 2.09Bzw2 basic leucine zipper and W2 domains 2 2.09Polb polymerase 2.08Coq5 coenzyme Q5 homolog, methyltransferase 2.08Tmsb10 thymosin, beta 10 2.07Ebna1bp2 EBNA1 binding protein 2 2.06Klhl2 kelch-like 2, Mayven 2.06Acvr2a activin receptor IIA 2.06Cpsf1 cleavage and polyadenylation specific factor 1 2.05Sepn1 selenoprotein N, 1 2.05Csf2ra colony stimulating factor 2 receptor, alpha, low-affinity 2.05Tsen2 tRNA splicing endonuclease 2 homolog 2.05Srp14 signal recognition particle 14 2.04BC056474 cDNA sequence BC056474 2.04Fbxo6 F-box protein 6 2.03Bloc1s1 biogenesis of lysosome-related organelles complex-1, subunit 1 2.02Mrpl54 mitochondrial ribosomal protein L54 2.02Mapk12 mitogen-activated protein kinase 12 2.01Rnps1 ribonucleic acid binding protein S1 2.01Sox12 SRY-box containing gene 12 2.01Mmab methylmalonic aciduria (cobalamin deficiency) type B homolog (human) 2.01Cdc42se2 CDC42 small effector 2 2Ebna1bp2 EBNA1 binding protein 2 2Chl1 cell adhesion molecule with homology to L1CAM 21500010J02Rik RIKEN cDNA 1500010J02 gene 1.991700037H04Rik RIKEN cDNA 1700037H04 gene 1.99Mctp1 multiple C2 domains, transmembrane 1 1.99Plch2 phospholipase C, eta 2 1.98Slc9a6 solute carrier family 9 (sodium/hydrogen exchanger), member 6 1.98Acot8 acyl-CoA thioesterase 8 1.96Igsf11 immunoglobulin superfamily, member 11 1.96Muted muted 1.96Epha7 Eph receptor A7 1.96Cadm3 cell adhesion molecule 3 1.96

St6galnac5ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1, 3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 1.94

Igfbp2 insulin-like growth factor binding protein 2 1.94Frmd3 FERM domain-containing protein 3 1.94Gm1673 gene model 1673, 1.93Fkbp5 FK506 binding protein 5 1.92

Smarcc1SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 1.92

BC004004 cDNA sequence BC004004 1.91Rwdd3 RWD domain containing 3 1.91A030009H04Rik RIKEN cDNA A030009H04 gene 1.9Unc45a unc-45 homolog A 1.9Trim9 tripartite motif protein 9 1.9Setd6 SET domain containing 6 1.89Unc5c unc-5 homolog C 1.89Ercc1 excision repair cross-complementing rodent repair deficiency, complementation group 1 1.89BC068157 cDNA sequence BC068157 1.87Mapbpip mitogen-activated protein binding protein interacting protein 1.87Aloxe3 arachidonate lipoxygenase 3 1.87Asb3 ankyrin repeat and SOCS box-containing protein 3 1.87

BC021381 cDNA sequence BC021381 1.86Th1l TH1-like homolog 1.86Alcam activated leukocyte cell adhesion molecule 1.85Gm1673 gene model 1673, 1.84U2af2 U2 small nuclear ribonucleoprotein auxiliary factor 1.84Zcchc3 zinc finger, CCHC domain containing 3 1.84Emr1 EGF-like module containing, mucin-like, hormone receptor-like sequence 1 1.83Acsf3 acyl-CoA synthetase family member 3 1.83LOC100048083 similar to Tect2 1.82Gpr162 G protein-coupled receptor 162 1.82Nup88 nucleoporin 88 1.82Tdrd7 tudor domain containing 7 1.81Mrps10 mitochondrial ribosomal protein S10 1.81Med11 mediator of RNA polymerase II transcription, subunit 11 homolog 1.81Trps1 trichorhinophalangeal syndrome I 1.8Ifngr1 interferon gamma receptor 1 1.82410022L05Rik RIKEN cDNA 2410022L05 gene 1.8Grip1 glutamate receptor interacting protein 1 1.8Cog1 component of oligomeric golgi complex 1 1.79Chchd8 coiled-coil-helix-coiled-coil-helix domain containing 8 1.79E4f1 E4F transcription factor 1 1.79Elmo1 engulfment and cell motility 1, ced-12 homolog 1.78Rapgef5 Rap guanine nucleotide exchange factor 1.782510006D16Rik RIKEN cDNA 2510006D16 gene 1.78Ldoc1l leucine zipper, down-regulated in cancer 1-like 1.77Tsr2 TSR2, 20S rRNA accumulation, homolog 1.77Fam126a Family with sequence similarity 126, member A 1.77LOC100048046 similar to SRp25 nuclear protein 1.761110039B18Rik RIKEN cDNA 1110039B18 gene 1.76Psmc3ip proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein 1.75Fahd2a fumarylacetoacetate hydrolase domain containing 2A 1.75Mbd4 methyl-CpG binding domain protein 4 1.75Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 1.74Zfp385a zinc finger protein 385A 1.74B230373P09Rik RIKEN cDNA B230373P09 gene 1.74Bzw2 basic leucine zipper and W2 domains 2 1.741700030K09Rik RIKEN cDNA 1700030K09 gene 1.74Ncdn neurochondrin 1.74Samd10 sterile alpha motif domain containing 10 1.73Apitd1 apoptosis-inducing, TAF9-like domain 1 1.73Lrpap1 low density lipoprotein receptor-related protein associated protein 1 1.73Tmsb10 thymosin, beta 10 1.73Hn1 hematological and neurological expressed sequence 1 1.73Zbtb39 zinc finger and BTB domain containing 39 1.73Ahdc1 AT hook, DNA binding motif, containing 1 1.73Chchd5 coiled-coil-helix-coiled-coil-helix domain containing 5 1.72Ccdc23 coiled-coil domain containing 23 1.72Pum1 pumilio 1 1.72Lrwd1 eucine-rich repeats and WD repeat domain containing 1 1.72Xrcc6 X-ray repair complementing defective repair in Chinese hamster cells 6 1.72Lgtn ligatin 1.72Zfp318 zinc finger protein 318 1.711810009N02Rik RIKEN cDNA 1810009N02 gene 1.71Hook2 hook homolog 2 1.71

Pfdn2 prefoldin 2 1.71Scamp3 Secretory carrier membrane protein 3 1.71Lrrc16b leucine rich repeat containing 16B 1.7Cntn6 contactin 6 1.7LOC100044159 hypothetical protein LOC100044159 1.7Mettl1 methyltransferase-like 1 1.69Pfn1 profilin 1 1.69Atp2b4 ATPase, Ca++ transporting, plasma membrane 4 1.69Dmrtc1a DMRT-like family C1a 1.68Mtap7d1 microtubule-associated protein 7 domain containing 1 1.68Dlx1 distal-less homeobox 1 1.68Kcnip3 Kv channel interacting protein 3, calsenilin 1.67Dhcr7 7-dehydrocholesterol reductase 1.67Hcn3 hyperpolarization-activated, cyclic nucleotide-gated K+ 3 1.66Mccc1 methylcrotonoyl-Coenzyme A carboxylase 1 1.66Nhlrc1 NHL repeat containing 1 1.66Ddx51 DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 1.66Fgfrl1 fibroblast growth factor receptor-like 1 1.65Mybbp1a MYB binding protein (P160) 1a 1.65Prkrip1 Prkr interacting protein 1 (IL11 inducible) 1.65Ssh3 slingshot homolog 3 1.65Calca calcitonin/calcitonin-related polypeptide, alpha 1.64Lst1 Leukocyte specific transcript 1 1.64Smyd3 SET and MYND domain containing 3 1.64Efs embryonal Fyn-associated substrate 1.63Pom121 nuclear pore membrane protein 121 1.63Abcg1 ATP-binding cassette, sub-family G 1.63Zxdc ZXD family zinc finger C 1.63Stmn1 Stathmin 1 1.63Prdx5 peroxiredoxin 5 1.63Trpt1 tRNA phosphotransferase 1 1.62Aurkaip1 aurora kinase A interacting protein 1 1.62Sla src-like adaptor 1.62Gsk3a glycogen synthase kinase 3 alpha 1.62Caln1 calneuron 1 1.62 Naa60 N(alpha)-acetyltransferase 60, NatF catalytic subunit 1.62Pak3 p21 (CDKN1A)-activated kinase 3 1.62Nsmce1 non-SMC element 1 homolog 1.61Tha1 threonine aldolase 1 1.616720458F09Rik RIKEN cDNA 6720458F09 gene 1.61Kif21b kinesin family member 21B 1.6St7 Suppression of tumorigenicity 7 1.6Bola2 bolA-like 2 1.59Spire2 spire homolog 2 1.59Ifitm2 interferon induced transmembrane protein 2 1.59Slc39a6 solute carrier family 39 (metal ion transporter), member 6 1.59Gtpbp6 GTP binding protein 6 1.59Rin3 Ras and Rab interactor 3 1.59Map3k3 mitogen-activated protein kinase kinase kinase 3 1.59Tmem117 transmembrane protein 117 1.59Hist1h2bk histone cluster 1, H2bk 1.58Ovgp1 oviductal glycoprotein 1 1.58Rai14 retinoic acid induced 14 1.58Rnps1 Ribonucleic acid binding protein S1 1.58

Galntl4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 1.58Eno1 enolase 1, alpha non-neuron 1.57Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor 1 1.57Mtvr2 Family with sequence similarity 89, member B 1.56Zfp692 zinc finger protein 692 1.56Igsf21 immunoglobin superfamily, member 21 1.56Sec14l1 SEC14-like 1 1.56Banf1 barrier to autointegration factor 1 1.55Rps6ka1 ribosomal protein S6 kinase polypeptide 1 1.55Efemp2 epidermal growth factor-containing fibulin-like extracellular matrix protein 2 1.54Psme1 proteasome (prosome, macropain) 28 subunit, alpha 1.54Fkbp2 FK506 binding protein 2 1.54Slc5a6 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 1.54Mtx1 metaxin 1 1.54Tgs1 trimethylguanosine synthase homolog 1.54Nme3 non-metastatic cells 3, protein expressed in 1.53Spsb4 splA/ryanodine receptor domain and SOCS box containing 4 1.53Gspt2 G1 to S phase transition 2 1.53Lca5 Leber congenital amaurosis 5 1.52Actr8 ARP8 actin-related protein 8 homolog 1.52Trappc4 trafficking protein particle complex 4 1.52Syf2 SYF2 homolog, RNA splicing factor 1.52Fpgs folylpolyglutamyl synthetase 1.52Tmem107 transmembrane protein 107 1.511700025G04Rik RIKEN cDNA 1700025G04 gene 1.51Tctex1d2 Tctex1 domain containing 2 1.51Pacsin1 protein kinase C and casein kinase substrate in neurons 1 1.51Scarb2 scavenger receptor class B, member 2 1.5Haghl Hydroxyacylglutathione hydrolase-like 1.5Gabrd gamma-aminobutyric acid (GABA-A) receptor, subunit delta -1.5Ccdc127 coiled-coil domain containing 127 -1.5Mdga2 MAM domain containing glycosylphosphatidylinositol anchor 2 -1.51Stard8 START domain containing 8 -1.51Inppl1 inositol polyphosphate phosphatase-like 1 -1.52Acsl6 acyl-CoA synthetase long-chain family member 6 -1.52Igfbp6 insulin-like growth factor binding protein 6 -1.52Klhdc3 kelch domain containing 3 -1.53Rgs6 regulator of G-protein signaling 6 -1.53Yaf2 YY1 associated factor 2 -1.54Dmrta2 doublesex and mab-3 related transcription factor like family A2 -1.55LOC100047651 similar to FOG -1.55Trnp1 TMF1-regulated nuclear protein 1 -1.55Mrpl9 mitochondrial ribosomal protein L9 -1.56Ndufaf1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 -1.56Prei4 preimplantation protein 4 -1.57Itm2a integral membrane protein 2A -1.57Acsl6 acyl-CoA synthetase long-chain family member 6 -1.57Plekhg3 pleckstrin homology domain containing, family G (with RhoGef domain) member 3 -1.57Epdr1 ependymin related protein 1 -1.58Bzw1 Basic leucine zipper and W2 domains 1 -1.59Dguok deoxyguanosine kinase -1.59Irf9 interferon regulatory factor 9 -1.59Ppm1b protein phosphatase 1B, magnesium dependent, beta isoform -1.59Srd5a3 steroid 5 alpha-reductase 3 -1.6

Idh3g isocitrate dehydrogenase 3 (NAD+), gamma -1.6Cyb5r4 cytochrome b5 reductase 4 -1.61Nars asparaginyl-tRNA synthetase -1.61Faah fatty acid amide hydrolase -1.61Gpr34 G protein-coupled receptor 34 -1.62Arhgap15 Rho GTPase activating protein 15 -1.62Lass2 LAG1 homolog, ceramide synthase 2 -1.62Stt3b STT3, subunit of the oligosaccharyltransferase complex, homolog B -1.63Necab3 N-terminal EF-hand calcium binding protein 3 -1.63Trak2 trafficking protein, kinesin binding 2 -1.63Fbxo7 F-box only protein 7 -1.63Napa N-ethylmaleimide sensitive fusion protein attachment protein alpha -1.63

Herpud1homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 -1.64

Wdr8 WD repeat domain 8 -1.64Ncald neurocalcin delta -1.64Rtn4 reticulon 4 -1.651700084C01Rik RIKEN cDNA 1700084C01 gene -1.65Lmna lamin A, transcript variant 2 -1.66Ugt8a UDP galactosyltransferase 8A -1.66Snrpd1 small nuclear ribonucleoprotein D1 -1.67Camkk1 calcium/calmodulin-dependent protein kinase kinase 1, alpha -1.67Lrrc6 leucine rich repeat containing 6 -1.67AW549877 expressed sequence AW549877 -1.68Spire1 spire homolog 1 -1.682310016C16Rik RIKEN cDNA 2310016C16 gene -1.68Pcgf5 polycomb group ring finger 5 -1.68Golm1 golgi membrane protein 1 -1.69Mtap7d2 MAP7 domain containing 2 -1.69Actb actin, beta, cytoplasmic -1.69Asf1a ASF1 anti-silencing function 1 homolog A -1.69Lass2 LAG1 homolog, ceramide synthase 2 -1.7Fbxo27 F-box protein 27 -1.7Cacng2 calcium channel, voltage-dependent, gamma subunit 2 -1.714933421E11Rik RIKEN cDNA 4933421E11 gene -1.71Kbtbd2 kelch repeat and BTB (POZ) domain containing 2 -1.71Ociad1 OCIA domain containing 1 -1.72Bcor Bcl6 interacting corepressor -1.72Kcnb1 potassium voltage gated channel, Shab-related subfamily, member 1 -1.73Ociad1 OCIA domain containing 1 -1.73Evi2a ecotropic viral integration site 2a -1.73Fbxo32 F-box protein 32 -1.73Gria3 glutamate receptor, ionotropic, AMPA3 -1.74Iars2 isoleucine-tRNA synthetase 2, mitochondrial -1.74Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform -1.74Gpx8 glutathione peroxidase 8 -1.75LOC382010 Similar to KIAA1712 protein -1.76Dctd dCMP deaminase -1.76Mfn2 mitofusin 2 -1.76Rpl7l1 ribosomal protein L7-like 1 -1.77Slc12a2 solute carrier family 12, member 2 -1.77Fa2h fatty acid 2-hydroxylase -1.78Rgs7 regulator of G protein signaling 7 -1.78Mgll monoglyceride lipase -1.78Msc musculin -1.78

Dpm1 Dolichol-phosphate (beta-D) mannosyltransferase 1 -1.8Hebp1 heme binding protein 1 -1.8Psat1 phosphoserine aminotransferase 1 -1.8Mapk10 mitogen-activated protein kinase 10 -1.8Anxa3 annexin A3 -1.8Jdp2 Jun dimerization protein 2 -1.81Cadm2 cell adhesion molecule 2 -1.81Lmna lamin A -1.84Mrpl48 mitochondrial ribosomal protein L48 -1.84Aldh1l1 aldehyde dehydrogenase 1 family, member L1 -1.85Ap1s1 adaptor protein complex AP-1, sigma 1 -1.86Wdr22 DDB1 and CUL4 associated factor 5 -1.86Ppm1m protein phosphatase 1M -1.86Scd2 stearoyl-Coenzyme A desaturase 2 -1.86Fez2 Fasciculation and elongation protein zeta 2 -1.87Pde1a phosphodiesterase 1A, calmodulin-dependent -1.87Kcnk4 potassium channel, subfamily K, member 4 -1.88Stmn4 stathmin-like 4 -1.88Trak2 trafficking protein, kinesin binding 2 -1.88Cadps2 Ca2+-dependent activator protein for secretion 2 -1.88Ttc17 tetratricopeptide repeat domain 17 -1.88Ppm2c protein phosphatase 2C, magnesium dependent, catalytic subunit -1.88Enpp6 ectonucleotide pyrophosphatase/phosphodiesterase 6 -1.89Fndc5 fibronectin type III domain containing 5 -1.92310016C16Rik RIKEN cDNA 2310016C16 gene -1.9Pebp1 phosphatidylethanolamine binding protein 1 -1.91Ifit3 interferon-induced protein with tetratricopeptide repeats 3 -1.91Arid4b AT rich interactive domain 4B (Rbp1 like), transcript variant 2 -1.91Gabrg2 gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2, transcript variant 2 -1.91Hexdc hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing -1.92Stk17b serine/threonine kinase 17b -1.93Eif2ak1 Eukaryotic translation initiation factor 2 alpha kinase 1 -1.93Rab3b RAB3B, member RAS oncogene family -1.93Mkks McKusick-Kaufman syndrome protein -1.93Cdc2l1 cell division cycle 2-like 1 -1.94Parva parvin, alpha -1.94Snrpd1 small nuclear ribonucleoprotein D1 -1.96Hist1h1c histone cluster 1, H1c -1.96Wipi1 WD repeat domain, phosphoinositide interacting 1 -1.96Msi2h Musashi RNA-binding protein homolog 2 -1.97Kcnu1 potassium channel, subfamily U, member 1 -1.97Stmn4 stathmin-like 4 -1.98Sbds Shwachman-Bodian-Diamond syndrome homolog -1.98C130038G02Rik RIKEN cDNA C130038G02 gene -1.98Frmd6 FERM domain containing 6 -1.99Rab5a RAB5A, member RAS oncogene family -1.99Dpm1 dolichol-phosphate (beta-D) mannosyltransferase 1 -1.99Arhgap15 Rho GTPase activating protein 15 -2Copa coatomer protein complex subunit alpha -2Fgfr1op2 FGFR1 oncogene partner 2 -2Mobp myelin-associated oligodendrocytic basic protein -2Pou6f1 POU domain, class 6, transcription factor 1 -2Tceb1 transcription elongation factor B -2.01Exoc2 exocyst complex component 2 -2.01

Dfna5h deafness, autosomal dominant 5 homolog -2.01Krt222 keratin 222 -2.02Ufc1 ubiquitin-fold modifier conjugating enzyme 1 -2.02Pla2g7 phospholipase A2, group VII -2.02Zbtb17 zinc finger and BTB domain containing 17 -2.02Strbp spermatid perinuclear RNA binding protein -2.02Ephb6 Eph receptor B6 -2.02Pik3r4 phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 -2.02Lix1 Protein limb expression 1 homolog -2.03Ddef2 development and differentiation enhancing factor 2 -2.05Grb14 growth factor receptor bound protein 14 -2.06LOC100046035 similar to ADAM23 -2.01Pcmt1 protein-L-isoaspartate -2.07Ola1 Obg-like ATPase 1 -2.08Cnp 2',3'-cyclic nucleotide 3' phosphodiesterase -2.08Pde1a phosphodiesterase 1A, calmodulin-dependent -2.09Cyp51 cytochrome P450, family 51 -2.09Iigp2 interferon inducible GTPase 2 -2.09Dbc1 deleted in bladder cancer 1 -2.09Rims1 regulating synaptic membrane exocytosis 1 -2.1Pdzph1 PDZ and pleckstrin homology domains 1 -2.11Tmem49 transmembrane protein 49 -2.12E2f1 E2F transcription factor 1 -2.14Rab6 RAB6, member RAS oncogene family -2.14Mag myelin-associated glycoprotein -2.14Tek endothelial-specific receptor tyrosine kinase -2.15Napa N-ethylmaleimide sensitive fusion protein attachment protein alpha -2.16Slc40a1 solute carrier family 40 -2.16B2m beta-2 microglobulin -2.18Ppox protoporphyrinogen oxidase -2.18Ddr1 discoidin domain receptor family, member 1 -2.18Atf4 activating transcription factor 4 -2.2Crebl2 cAMP responsive element binding protein-like 2 -2.22Lsm12 LSM12 homolog -2.228430419L09Rik RIKEN cDNA 8430419L09 gene -2.22Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1 -2.23Emp2 epithelial membrane protein 2 -2.23Ccdc132 coiled-coil domain containing 132 -2.23Ep300 E1A binding protein p300 -2.24Zfp828 zinc finger protein 828 -2.25Med23 mediator complex subunit 23 -2.26Darc Duffy blood group, chemokine receptor -2.26Anxa3 annexin A3 -2.26Hsd17b7 hydroxysteroid (17-beta) dehydrogenase 7 -2.27Snx13 sorting nexin 13 -2.28Rab6 RAB6, member RAS oncogene family -2.29LOC100046781 similar to carboxypeptidase D -2.29Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -2.29Hexdc hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing -2.3Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 -2.31Ccndbp1 cyclin D-type binding-protein 1 -2.31Rora RAR-related orphan receptor alpha -2.31Pex19 peroxisome biogenesis factor 19 -2.32Ncam2 neural cell adhesion molecule 2 -2.32

Igtp interferon gamma induced GTPase -2.32Lix1 limb expression 1 homolog -2.32Lgi3 leucine-rich repeat LGI family, member 3 -2.33Abcd3 ATP-binding cassette, sub-family D -2.33Srd5a3 steroid 5 alpha-reductase 3 -2.34B2m beta-2 microglobulin -2.34Cacnb4 calcium channel, voltage-dependent, beta 4 subunit -2.35Arl6ip1 ADP-ribosylation factor-like 6 interacting protein 1 -2.35Acaa2 acetyl-Coenzyme A acyltransferase 2 -2.35Ivd isovaleryl coenzyme A dehydrogenase -2.36Frag1 FGF receptor activating protein 1 -2.37Ing3 inhibitor of growth family, member 3 -2.37Them4 thioesterase superfamily member 4 -2.37Bcas1 breast carcinoma amplified sequence 1 -2.37Cited4 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 -2.38Iqgap1 IQ motif containing GTPase activating protein 1 -2.38C1qdc2 C1q domain containing 2 -2.39D1Bwg0212e DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed -2.39Ykt6 YKT6 homolog -2.41Tssc1 tumor suppressing subtransferable candidate 1 -2.41Mat2b methionine adenosyltransferase II, beta -2.42Socs6 suppressor of cytokine signaling 6 -2.43Slc45a1 solute carrier family 45, member 1 -2.45Bok BCL2-related ovarian killer -2.45Med8 mediator of RNA polymerase II transcription, subunit 8 homolog -2.45LOC100046039 similar to histone deacetylase HD1 -2.46Ufsp2 UFM1-specific peptidase 2 -2.46Centg1 centaurin, gamma 1 -2.48Nos1ap nitric oxide synthase 1 (neuronal) adaptor protein -2.49Rsrc2 arginine/serine-rich coiled-coil 2 -2.49Rab6 RAB6, member RAS oncogene family -2.49Zfp326 zinc finger protein 326 -2.5Ubxd4 UBX domain containing 4 -2.5Fbxo44 F-box protein 44 -2.5Syt1 Synaptotagmin I -2.52Akap7 A kinase (PRKA) anchor protein 7 -2.55Ifngr2 interferon gamma receptor 2 -2.56Rshl2a radial spokehead-like 2A -2.58Rtn4 Reticulon 4 -2.58Rabl2a RAB, member of RAS oncogene family-like 2A -2.58Rcan2 regulator of calcineurin 2 -2.59Ttyh2 tweety homolog 2 -2.59Cbwd1 COBW domain containing 1 -2.6Pgm2l1 Phosphoglucomutase 2-like 1 -2.61Lims2 LIM and senescent cell antigen like domains 2 -2.63Socs2 suppressor of cytokine signaling 2 -2.63Camkk2 calcium/calmodulin-dependent protein kinase kinase 2, beta -2.65Rpap1 RNA polymerase II associated protein 1 -2.66Caskin1 CASK interacting protein 1 -2.66Hr hairless -2.681700040I03Rik RIKEN cDNA 1700040I03 gene -2.68Atf7ip activating transcription factor 7 interacting protein -2.68Dusp7 dual specificity phosphatase 7 -2.68Eps15 epidermal growth factor receptor pathway substrate 15 -2.68

Gpr135 G protein-coupled receptor 135 -2.69Slc35a5 solute carrier family 35, member A5 -2.74Cbx5 chromobox homolog 5 -2.74Ank2 ankyrin 2, brain -2.74Kcnip4 Kv channel interacting protein 4 -2.75Ttyh2 Tweety homolog 2 -2.76AI316807 expressed sequence AI316807 -2.78Itpka Inositol 1,4,5-trisphosphate 3-kinase A -2.78Myl4 myosin, light polypeptide 4 -2.78Ociad2 OCIA domain containing 2 -2.8Dhrsx dehydrogenase/reductase (SDR family) X chromosome -2.81Rasgrp1 RAS guanyl releasing protein 1 -2.81Gpr17 G protein-coupled receptor 17 -2.81Pak7 p21 -2.84D4Ertd617e DNA segment, Chr 4, ERATO Doi 617, expressed , long non-coding RNA -2.84Lgals3bp lectin, galactoside-binding, soluble, 3 binding protein -2.86Zfp160 zinc finger protein 160 -2.87Grin1 glutamate receptor, ionotropic, NMDA1 -2.88Nudt4 nudix -2.9Trem2 triggering receptor expressed on myeloid cells 2 -2.9Klhl9 kelch-like 9 -2.91Aldh1a1 aldehyde dehydrogenase family 1, subfamily A1 -2.91Vangl2 vang-like 2 (van gogh, Drosophila) -2.93Ramp3 receptor (calcitonin) activity modifying protein 3 -2.96Zfp101 zinc finger protein 101 -2.96Tsc2 tuberous sclerosis 2 -2.96Cldn11 claudin 11 -2.97Rapgef4 Rap guanine nucleotide exchange factor (GEF) 4 -2.97Lrg1 leucine-rich alpha-2-glycoprotein 1 -3Bckdhb branched chain ketoacid dehydrogenase E1, beta polypeptide -3.01Rock2 Rho-associated coiled-coil containing protein kinase 2 -3.02Pdlim2 PDZ and LIM domain 2 -3.02Rnf41 ring finger protein 41 -3.02Kif5a kinesin family member 5A -3.04Camk1d calcium/calmodulin-dependent protein kinase ID -3.05Ctsk cathepsin K -3.07LOC100044190 hypothetical protein LOC100044190 -3.08Slc35a5 solute carrier family 35, member A5 -3.12Gria3 glutamate receptor, ionotropic, AMPA3 -3.14Hnrph2 heterogeneous nuclear ribonucleoprotein H2 -3.16Pdlim2 PDZ and LIM domain 2 -3.16Zfp91 zinc finger protein 91 -3.17AI593442 expressed sequence AI593442 -3.17Lypd6b LY6/PLAUR domain containing 6B -3.21Fam82a2 family with sequence similarity 82, member A2 -3.21Kif5a kinesin family member 5A -3.21LOC100043257 similar to RNA binding motif protein 3 -3.24Fndc5 fibronectin type III domain containing 5 -3.26Actl6b actin-like 6B -3.27Yipf4 Yip1 domain family, member 4 -3.3Extl2 exostoses (multiple)-like 2 -3.33Pigz phosphatidylinositol glycan anchor biosynthesis, class Z -3.34Csnrp3 cysteine-serine-rich nuclear protein 3 -3.36Snapc3 small nuclear RNA activating complex, polypeptide 3 -3.36

Rnf41 ring finger protein 41 -3.38Sytl2 synaptotagmin-like 2 -3.39Tspan2 tetraspanin 2 -3.39Ddx6 DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 -3.43Arid5b AT rich interactive domain 5B (Mrf1 like) -3.44Morf4l1 mortality factor 4 like 1 -3.44Cd200 CD200 antigen -3.45Pik3ca phosphatidylinositol 3-kinase, catalytic, alpha polypeptide -3.47Zfp238 zinc finger protein 238 -3.5Mrps7 mitchondrial ribosomal protein S7 -3.54Ccdc85a coiled-coil domain containing 85A -3.56Tmprss7 transmembrane serine protease 7 -3.57Pdlim2 PDZ and LIM domain 2 -3.57AI593442 expressed sequence AI593442 -3.58Lars2 leucyl-tRNA synthetase, mitochondrial -3.6Llgl2 lethal giant larvae homolog 2 -3.6Fastkd3 FAST kinase domains 3 -3.61Tmem10 transmembrane protein 10 -3.63Sbf1 SET binding factor 1 -3.63Cort cortistatin -3.64Rorb RAR-related orphan receptor beta -3.64Zfp691 zinc finger protein 691 -3.64Hbb-b1 hemoglobin, beta adult major chain -3.67Rcan2 regulator of calcineurin 2 -3.72Dkk3 dickkopf homolog 3 (Xenopus laevis) -3.76BC017643 cDNA sequence BC017643 -3.78Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -3.81Tmem132d transmembrane protein 132D -3.84Atp2c1 ATPase, Ca++-sequestering -3.9Zmynd11 zinc finger, MYND domain containing 11 -3.93Cort cortistatin -3.95Zmynd11 Zinc finger, MYND domain containing 11 -3.95Med23 mediator complex subunit 23 -3.96Rnmt RNA (guanine-7-) methyltransferase -3.98Cnksr2 connector enhancer of kinase suppressor of Ras 2 -4Spire1 spire homolog 1 -4.01Mobp myelin-associated oligodendrocytic basic protein -4.03Zfp91-cntf zinc finger protein 91, ciliary neurotrophic factor transcription unit -4.05H2-T23 histocompatibility 2, T region locus 23 -4.06Tmc7 transmembrane channel-like gene family 7 -4.06Ash1l Ash1 (absent, small, or homeotic)-like (Drosophila) -4.07Bok Bcl-2-related ovarian killer protein -4.12Atpbd1b ATP binding domain 1 family, member B -4.14Kif21a kinesin family member 21A -4.15Zfp758 zinc finger protein 758 -4.21Plcb4 phospholipase C, beta 4 -4.21Tfrc transferrin receptor -4.23Cit citron -4.24Arhgef19 Rho guanine nucleotide exchange factor (GEF) 19 -4.25Mog myelin oligodendrocyte glycoprotein -4.27Mobp myelin-associated oligodendrocytic basic protein -4.28Ubtd2 ubiquitin domain containing 2 -4.33D17Wsu92e DNA segment, Chr 17, Wayne State University 92, expressed -4.34Ddx6 DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 -4.35

Creg2 cellular repressor of E1A-stimulated genes 2 -4.36Ankrd12 ankyrin repeat domain 12 -4.38Bach1 BTB and CNC homology 1 -4.4Flrt2 fibronectin leucine rich transmembrane protein 2 -4.43Zfp367 zinc finger protein 367 -4.431110057K04Rik RIKEN cDNA 1110057K04 gene -4.44Iap Intracisternal A particles -4.46Axud1 AXIN1 up-regulated 1 -4.49Tfrc transferrin receptor -4.5Mod1 malic enzyme, supernatant -4.59Mog myelin oligodendrocyte glycoprotein -4.61Zfp106 Zinc finger protein 106 -4.61Rasgrp1 RAS guanyl releasing protein 1 -4.68Pex19 peroxisome biogenesis factor 19 -4.71Ash1l ash1 (absent, small, or homeotic)-like (Drosophila) -4.71LOC545056 ubiquitin-conjugating enzyme E2, J2 homolog pseudogene -4.74Spag9 sperm associated antigen 9, transcript variant 3 -4.79Mef2c myocyte enhancer factor 2C -4.79Klhl21 kelch-like 21 -4.83Tagap T-cell activation Rho GTPase-activating protein -4.87Rpl29 ribosomal protein L29 -4.87Tex9 testis expressed gene 9 -4.88Epm2aip1 EPM2A (laforin) interacting protein 1 -4.89Arl5a ADP-ribosylation factor-like 5A -4.93Agxt2l1 alanine-glyoxylate aminotransferase 2-like 1 -5.01Bsdc1 BSD domain containing 1 -5.06Mal myelin and lymphocyte protein, T-cell differentiation protein -5.1Osbpl6 oxysterol binding protein-like 6 -5.15Fgfr1op2 FGFR1 oncogene partner 2 -5.19Slc25a18 solute carrier family 25 (mitochondrial carrier), member 18 -5.21Zfp106 zinc finger protein 106 -5.25Lsamp limbic system-associated membrane protein -5.43Abcd2 ATP-binding cassette, sub-family D (ALD), member 2 -5.44Agxt2l1 alanine-glyoxylate aminotransferase 2-like 1 -5.57Ccl27 chemokine (C-C motif) ligand 27, transcript variant 1 -5.58R3hdm1 R3H domain 1 (binds single-stranded nucleic acids) -5.66Rps15a ribosomal protein S15a -5.82Btbd3 BTB (POZ) domain containing 3 -5.95Abcd2 ATP-binding cassette, sub-family D -6.2Lrrc57 leucine rich repeat containing 57 -6.21Arl5a ADP-ribosylation factor-like 5A -5.925Atp8a1 ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1, transcript variant 2 -6.48Agpat5 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) -6.5Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 -6.54Ccp110 centriolar coiled coil protein 110 -6.57Scn1a Sodium channel, voltage-gated, type I, alpha polypeptide -6.61Nsd1 nuclear receptor-binding SET-domain protein 1 -7.3Zfp68 zinc finger protein 68 -7.33Kif1b kinesin family member 1B -7.34Zzz3 zinc finger, ZZ domain containing 3 -7.38Taf6 TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor -7.92LOC433801 similar to RIKEN cDNA 6330416L07 gene -8.02Kitl kit ligand -8.16Megf9 multiple EGF-like-domains 9 -8.18

Zbtb7a zinc finger and BTB domain containing 7a -8.29Atp2b2 ATPase, Ca++ transporting, plasma membrane 2 -8.35Zfp365 zinc finger protein 365 -8.66Cfap69 cilia and flagella associated protein 69 -9.25Clstn1 Calsyntenin 1 -9.55Nell2 NEL-like 2 -9.55Kcna1 potassium voltage-gated channel, shaker-related subfamily, member 1 -9.57Uap1 UDP-N-acetylglucosamine pyrophosphorylase 1 -9.6Ccng2 cyclin G2 -10.09Nell2 NEL-like 2 -10.99Actl6b actin-like 6B -11.05Nell2 NEL-like 2 -11.09Zfp68 zinc finger protein 68 -11.44B3galt6 UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 -11.72Ccl21c chemokine (C-C motif) ligand 21c (leucine) -12.31Ccl21b chemokine (C-C motif) ligand 21b -13.85

Table S2. Gene transcripts significantly and differentially regulated in T1R3KO hippocampus compared to WT control cortex. For each significantly-regulated transcript the Official Gene Symbol, transcript description and expression Z ratio, T1R3KO versus WT are represented.

Gene Symbol Description Z ratio T1R3KO:WTBC032265 cDNA sequence BC032265 13.56Tmem87a transmembrane protein 87A 11.09Stbd1 starch binding domain 1 10.35Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog 8.05Atp10d ATPase, Class V, type 10D 7.88Ndufb10 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 7.55Cap1 CAP, adenylate cyclase-associated protein 1 7.1Hyi hydroxypyruvate isomerase homolog 7.08Nubp2 nucleotide binding protein 2 6.83Fcgr3 Fc receptor, IgG, low affinity III 6.48Trim2 tripartite motif protein 2 6.26Rgs5 regulator of G-protein signaling 5 6.04Sfrp1 secreted frizzled-related sequence protein 1 6.02Cox18 COX18 cytochrome c oxidase assembly homolog 5.27Itgb3bp integrin beta 3 binding protein (beta3-endonexin) 5.05Cdkal1 threonylcarbamoyladenosine tRNA methylthiotransferase 4.96Mns1 meiosis-specific nuclear structural protein 1 4.94Scp2 sterol carrier protein 2, liver 4.93Adamts4 A disintegrin and metalloproteinase with thrombospondin motifs 4 4.6Nubp2 nucleotide binding protein 2 4.58Cd74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), transcript variant 2 4.432310002B06Rik RIKEN cDNA 2310002B06 gene 4.34Sdhc succinate dehydrogenase complex, subunit C, integral membrane protein 4.31Wars2 tryptophanyl tRNA synthetase 2 4.27Clec16a C-type lectin domain family 16, member A 4.26Rbm45 RNA binding motif protein 45 4.22Vars2 valyl-tRNA synthetase 2, mitochondrial 4.11Alad aminolevulinate, delta-, dehydratase 4.09Igsf1 immunoglobulin superfamily, member 1 4.06Asah3l N-acylsphingosine amidohydrolase 3-like 4.05Wbp11 WW domain binding protein 11 4.03BC048546 cDNA sequence BC048546 4.02Hdhd3 haloacid dehalogenase-like hydrolase domain containing 3 3.96Dnaic1 dynein, axonemal, intermediate chain 1 3.92Vps33a vacuolar protein sorting 33A 3.89Nts neurotensin 3.87Rbm43 RNA binding motif protein 43 3.82Osbpl3 oxysterol binding protein-like 3 3.78Hmgn2 high mobility group nucleosomal binding domain 2 3.75Cpne9 copine family member IX 3.66BC048546 cDNA sequence BC048546 3.62Ogn osteoglycin 3.61Aurkaip1 aurora kinase A interacting protein 1 3.59Vps33a vacuolar protein sorting 33A 3.55Gpr19 G protein-coupled receptor 19 3.53Mrps10 mitochondrial ribosomal protein S10 3.516330406I15Rik RIKEN cDNA 6330406I15 gene 3.51

Snx30 sorting nexin family member 30 3.48Phactr4 phosphatase and actin regulator 4 3.46C4b complement component 4B 3.44Usf1 upstream transcription factor 1 3.38Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase 3.38Tmem91 transmembrane protein 91 3.34D8Ertd82e DNA segment, Chr 8, ERATO Doi 82, expressed 3.26Gfer growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration) 3.24Dapk1 death associated protein kinase 1 3.22LOC677317 similar to Mod1 protein, transcript variant 4 3.22Lbh limb-bud and heart 3.17Rerg RAS-like, estrogen-regulated, growth-inhibitor 3.15Gins4 GINS complex subunit 4 3.14Uchl5 ubiquitin carboxyl-terminal esterase L5 3.13Ttl tubulin tyrosine ligase 3.11Nefm neurofilament, medium polypeptide 3.1Tspo translocator protein 3.09Cadm3 cell adhesion molecule 3 3.07Coq5 coenzyme Q5 homolog, methyltransferase 2.98Wnt2 wingless-related MMTV integration site 2 2.98Igsf1 immunoglobulin superfamily, member 1 2.94Extl1 exostoses 2.93Mrps10 mitochondrial ribosomal protein S10 2.92B4galt3 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 2.85Ahdc1 AT hook, DNA binding motif, containing 1 2.85Trib2 tribbles homolog 2 (Drosophila) 2.83Sncg synuclein, gamma 2.83Gnb1 guanine nucleotide binding protein (G protein), beta 1 2.79Pgam2 phosphoglycerate mutase 2 2.78Pea15 phosphoprotein enriched in astrocytes 15, transcript variant 1 2.77Nptx2 neuronal pentraxin 2 2.75Coq5 coenzyme Q5 homolog, methyltransferase 2.71LOC100045304 similar to neurofilament protein 2.76330406I15Rik RIKEN cDNA 6330406I15 gene 2.69Cox18 COX18 cytochrome c oxidase assembly homolog 2.69Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 2.68Smc5l1 structural maintenance of chromosomes 5, transcript variant 2 2.67Nudc nuclear distribution gene C homolog 2.67Nudc nuclear distribution gene C homolog 2.66Slc17a5 solute carrier family 17 (anion/sugar transporter), member 5 2.64Fbxo6 F-box protein 6 2.63Snapc3 small nuclear RNA activating complex, polypeptide 3 2.612610524H06Rik RIKEN cDNA 2610524H06 gene 2.6Col16a1 collagen, type XVI, alpha 1 2.59Habp4 hyaluronic acid binding protein 4 2.59Itgb4 integrin beta 4 2.57Pfdn2 prefoldin 2 2.56Emp1 epithelial membrane protein 1 2.52Tmsb4x thymosin, beta 4, X chromosome 2.52Pex6 peroxisomal biogenesis factor 6 2.5Cdh22 cadherin 22 2.5Top1mt DNA topoisomerase 1, mitochondrial 2.48Haghl Hydroxyacylglutathione hydrolase-like 2.48Olfml2b olfactomedin-like 2B 2.47

2210016L21Rik RIKEN cDNA 2210016L21 gene 2.44Fip1l1 FIP1 like 1 2.43Ebna1bp2 EBNA1 binding protein 2 2.41Ebna1bp2 EBNA1 binding protein 2 2.41Rreb1 ras responsive element binding protein 1 2.4Nol4 nucleolar protein 4 2.391810074P20Rik RIKEN cDNA 1810074P20 gene 2.31Arl6ip4 ADP-ribosylation factor-like 6 interacting protein 4 2.3Cidea cell death-inducing DNA fragmentation factor, alpha subunit-like effector A 2.3Unc5c unc-5 homolog C 2.29Nola3 nucleolar protein family A, member 3 2.29Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase 2.28A930025D01Rik RIKEN cDNA A930025D01 gene 2.085Bcl6 B-cell leukemia/lymphoma 6 2.27Gad1 glutamic acid decarboxylase 1 2.27N6amt1 N-6 adenine-specific DNA methyltransferase 1 2.25Tpd52l1 tumor protein D52-like 1 2.24Ndufb5 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 2.24Zfp692 zinc finger protein 692 2.24Pkp2 plakophilin 2 2.22Tmem74 transmembrane protein 74 2.21LOC100046996 similar to farnesyltransferase alpha subunit 2.21Scyl1 SCY1-like 1 2.19Pafah1b3 platelet-activating factor acetylhydrolase, isoform 1b, alpha1 subunit 2.171500032D16Rik RIKEN cDNA 1500032D16 gene 2.17Hectd2 HECT domain containing 2 2.16LOC100045780 similar to metalloprotease-disintegrin meltrin beta 2.16Ghitm growth hormone inducible transmembrane protein 2.15Sc5d sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) 2.14Moxd1 monooxygenase, DBH-like 1 2.14Rangrf RAN guanine nucleotide release factor 2.13Gmpr2 guanosine monophosphate reductase 2 2.12Col9a2 collagen, type IX, alpha 2 2.11Tsr2 TSR2, 20S rRNA accumulation, homolog 2.11Slc2a1 solute carrier family 2 (facilitated glucose transporter), member 1 2.1Ddo D-aspartate oxidase 2.09Pvalb parvalbumin 2.09Tesk1 testis specific protein kinase 1 2.09Gng10 guanine nucleotide binding protein (G protein), gamma 10 2.09Epha3 Eph receptor A3 2.073632451O06Rik RIKEN cDNA 3632451O06 gene 2.06Rftn1 raftlin lipid raft linker 1 2.05Sepn1 selenoprotein N, 1 2.04Polb polymerase (DNA directed), beta 2.04Socs2 suppressor of cytokine signaling 2 2.03Ntng1 netrin G1 2.02Magee1 melanoma antigen, family E, 1 2.02Tctex1d2 Tctex1 domain containing 2 2.02Raver2 ribonucleoprotein, PTB-binding 2 2.01Per2 period homolog 2 2.01Brdt bromodomain, testis-specific 2Golga3 golgi autoantigen, golgin subfamily a, 3 1.99D4Ertd22e DNA segment, Chr 4, ERATO Doi 22, expressed 1.99Inhba inhibin beta-A 1.99

Srxn1 sulfiredoxin 1 homolog 1.98Sec14l1 SEC14-like 1 1.98Dapk1 death associated protein kinase 1 1.97Abca3 ATP-binding cassette, sub-family A (ABC1), member 3, transcript variant 2 1.97Pcmt1 protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 1.97Cib2 calcium and integrin binding family member 2 1.96Rreb1 ras responsive element binding protein 1 1.95Ufsp1 UFM1-specific peptidase 1 1.95Vti1b vesicle transport through interaction with t-SNAREs 1B homolog 1.95Pmp22 peripheral myelin protein 1.94BC002163 cDNA sequence BC002163 1.94Sap130 Sin3A associated protein 1.93LOC100048046 similar to SRp25 nuclear protein, transcript variant 1 1.931810043G02Rik RIKEN cDNA 1810043G02 gene 1.93Creb3 cAMP responsive element binding protein 3 1.93Chrnb2 cholinergic receptor, nicotinic, beta polypeptide 2 1.92Poldip3 polymerase (DNA-directed), delta interacting protein 3 1.91Zmpste24 zinc metallopeptidase, STE24 homolog 1.91Zfp828 zinc finger protein 828 1.910610007P22Rik RIKEN cDNA 0610007P22 gene 1.91Rnps1 ribonucleic acid binding protein S1 1.9Ntn2l netrin 2-like 1.89Stard3 START domain containing 3 1.89Mpp6 membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) 1.89Cdkl2 cyclin-dependent kinase-like 2 (CDC2-related kinase), transcript variant 2 1.88Nme3 non-metastatic cells 3, protein expressed in 1.88Rgs12 regulator of G-protein signaling 12 1.87Zfp612 zinc finger protein 612 1.87Lefty1 left right determination factor 1 1.86Trappc1 trafficking protein particle complex 1 1.85Ppt1 palmitoyl-protein thioesterase 1 1.85Brsk2 BR serine/threonine kinase 2 1.84Smarcd3 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 1.84Abat 4-aminobutyrate aminotransferase 1.83Lgals8 lectin, galactose binding, soluble 8 1.831810009N02Rik RIKEN cDNA 1810009N02 gene 1.82Ost4 oligosaccharyltransferase 4 homolog (S. cerevisiae) 1.82Gmpr guanosine monophosphate reductase 1.81Chga chromogranin A 1.81Chga chromogranin A 1.81Nicn1 nicolin 1 1.8

Fkbp1b FK506 binding protein 1b 1.8Sumf2 sulfatase modifying factor 2 1.8Glrx2 glutaredoxin 2 (thioltransferase), transcript variant 1 1.8Col4a2 collagen, type IV, alpha 2 1.8Cdkl2 cyclin-dependent kinase-like 2 (CDC2-related kinase), transcript variant 2 1.79Lrrc33 leucine rich repeat containing 33 1.79Zcchc12 zinc finger, CCHC domain containing 12 1.78Aven apoptosis, caspase activation inhibitor 1.78Sdcbp syndecan binding protein 1.78Wnt7b wingless-related MMTV integration site 7B 1.77Atp13a4 ATPase type 13A4 1.77Hus1 Hus1 homolog 1.77Nhlrc1 NHL repeat containing 1 1.77

Psmb10 proteasome (prosome, macropain) subunit, beta type 10 1.76B020018G12Rik RIKEN cDNA B020018G12 gene 1.76Pftk1 PFTAIRE protein kinase 1 1.76Rnaseh2c ribonuclease H2, subunit C 1.75Mrps21 mitochondrial ribosomal protein S21 1.75Rhbdl7 rhomboid, veinlet-like 7 (Drosophila) 1.75Xpa xeroderma pigmentosum, complementation group A 1.75Tceb2 transcription elongation factor B 1.73Tmem85 transmembrane protein 85 1.73Prdm8 PR domain containing 8 1.73Grip1 glutamate receptor interacting protein 1 1.72Sec14l1 SEC14-like 1 1.72Rdm1 RAD52 motif 1 1.71Dtnb dystrobrevin, beta 1.7Habp4 hyaluronic acid binding protein 4 1.7Fabp3 fatty acid binding protein 3, muscle and heart 1.7Mybbp1a MYB binding protein (P160) 1a 1.7Frs3 fibroblast growth factor receptor substrate 3 1.7Mmab methylmalonic aciduria (cobalamin deficiency) type B homolog (human) 1.7Slc35b1 solute carrier family 35, member B1 1.69Epha7 Eph receptor A7 1.68C85492 expressed sequence C85492 1.68Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor 1 1.68Mtpn myotrophin 1.67Cnot7 CCR4-NOT transcription complex, subunit 7 1.67Arpc5 actin related protein 2/3 complex, subunit 5 1.67Banf1 barrier to autointegration factor 1 1.67Psip1 PC4 and SFRS1 interacting protein 1 1.66Cdc2l5 cell division cycle 2-like 5 1.66Pih1d1 PIH1 domain containing 1 1.661700037H04Rik RIKEN cDNA 1700037H04 gene 1.65LOC100039571 similar to ribosomal protein L23a 1.65Tnfrsf25 tumor necrosis factor receptor superfamily, member 25 1.65Polr2j polymerase 1.65Prps1 phosphoribosyl pyrophosphate synthetase 1 1.65B230342M21Rik RIKEN cDNA B230342M21 gene 1.64Pycrl pyrroline-5-carboxylate reductase-like 1.64Psma4 proteasome (prosome, macropain) subunit, alpha type 4 1.63Mrpl52 mitochondrial ribosomal protein L52 1.63Prkcd protein kinase C, delta 1.62Pex6 peroxisomal biogenesis factor 6 1.62Vkorc1l1 vitamin K epoxide reductase complex, subunit 1-like 1 1.61Elovl5 ELOVL family member 5, elongation of long chain fatty acids 1.61Txnl4a thioredoxin-like 4A 1.61Caly calcyon neuron-specific vesicular protein 1.61Sri sorcin 1.61Arl3 ADP-ribosylation factor-like 3 1.61Stk32c serine/threonine kinase 32C 1.6Lsm2 LSM2 homolog, U6 small nuclear RNA associated 1.6Appbp1 amyloid beta precursor protein binding protein 1 1.66330403K07Rik RIKEN cDNA 6330403K07 gene 1.59Palmd palmdelphin 1.59Grik1 glutamate receptor, ionotropic, kainate 1 1.59Gstp1 glutathione S-transferase, pi 1 1.59

Commd1 COMM domain containing 1 1.59Nrsn2 neurensin 2 1.59Hectd2 HECT domain containing 2 1.59Sec24d SEC24 related gene family, member D 1.59BC056474 cDNA sequence BC056474 1.592810428I15Rik RIKEN cDNA 2810428I15 gene 1.59Mrpl13 mitochondrial ribosomal protein L13 1.58Muted muted 1.58Lpl lipoprotein lipase 1.58Cdo1 cysteine dioxygenase 1, cytosolic 1.58LOC100044862 similar to Fbxl3 protein 1.58Plod3 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 1.57Prnpip1 prion protein interacting protein 1 1.57Abhd3 abhydrolase domain containing 3 1.57Dtna dystrobrevin alpha 1.56Mcph1 microcephaly, primary autosomal recessive 1 1.56Tgm2 transglutaminase 2, C polypeptide 1.56C1qbp complement component 1, q subcomponent binding protein 1.56Rps27l ribosomal protein S27-like 1.56Anapc2 anaphase promoting complex subunit 2 1.56Tdrd7 tudor domain containing 7 1.55Faim Fas apoptotic inhibitory molecule 1.55Chi3l1 chitinase 3-like 1 1.55Nagk N-acetylglucosamine kinase 1.55Lrrn1 leucine rich repeat protein 1, neuronal 1.55LOC676420 similar to ceramide kinases 1.54Cbr1 carbonyl reductase 1 1.54Prkab1 protein kinase, AMP-activated, beta 1 non-catalytic subunit 1.53Gpr162 G protein-coupled receptor 162 1.53Tspan17 tetraspanin 17 1.53Tnfrsf21 tumor necrosis factor receptor superfamily, member 21 1.53Cugbp2 CUG triplet repeat, RNA binding protein 2 1.53Elavl2 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B), transcript variant 3 1.52Med21 mediator complex subunit 21 1.52Ddx51 DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 1.52Ndufa8 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 1.52Copg coatomer protein complex, subunit gamma 1.52Robo1 roundabout homolog 1 1.51Vgf VGF nerve growth factor inducible 1.51Cox6c cytochrome c oxidase, subunit VIc 1.5Pknox2 Pbx/knotted 1 homeobox 2 -1.51Gde1 glycerophosphodiester phosphodiesterase 1 -1.51Pex11b peroxisomal biogenesis factor 11b -1.51Phldb1 pleckstrin homology-like domain, family B, member 1 -1.51Kcnc4 potassium voltage gated channel, Shaw-related subfamily, member 4 -1.51Pip5k1a phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha -1.51Lrif1 ligand dependent nuclear receptor interacting factor 1, transcript variant 3 -1.52Cux2 cut-like homeobox 2 -1.52Rassf1 Ras association -1.52Phka1 phosphorylase kinase alpha 1 -1.53BC046404 cDNA sequence BC046404 -1.53Tprkb Tp53rk binding protein -1.53Coq7 demethyl-Q 7 -1.53Trafd1 TRAF type zinc finger domain containing 1 -1.53

Faah fatty acid amide hydrolase -1.53Rab6 RAB6, member RAS oncogene family -1.54Wdr70 WD repeat domain 70, transcript variant 3 -1.55Wiz widely-interspaced zinc finger motifs -1.56LOC100045343 similar to CDNA sequence BC046404 -1.56Map2k3 mitogen activated protein kinase kinase 3 -1.56Fgf12 fibroblast growth factor 12 -1.57Zbtb17 zinc finger and BTB domain containing 17 -1.57Eprs glutamyl-prolyl-tRNA synthetase -1.58Fbrsl1 fibrosin-like 1, transcript variant 2 -1.58Ypel1 yippee-like 1 -1.58Prkcz protein kinase C, zeta -1.59Tmem132e transmembrane protein 132E -1.59Stard7 START domain containing 7 -1.59Kbtbd2 kelch repeat and BTB -1.59Pou6f1 POU domain, class 6, transcription factor 1 -1.59Bckdhb branched chain ketoacid dehydrogenase E1, beta polypeptide -1.6Ddx6 DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 -1.6Bzrap1 benzodiazapine receptor associated protein 1 -1.6Eif2ak1 eukaryotic translation initiation factor 2 alpha kinase 1 -1.6Nphp1 nephronophthisis 1 (juvenile) homolog (human) -1.6Adcy8 adenylate cyclase 8 -1.61Sesn1 sestrin 1 -1.61Cnp 2',3'-cyclic nucleotide 3' phosphodiesterase -1.61Clcn7 chloride channel 7 -1.63Cant1 calcium activated nucleotidase 1 -1.63Phactr1 phosphatase and actin regulator 1 -1.63Prpf39 PRP39 pre-mRNA processing factor 39 homolog -1.64Copa coatomer protein complex subunit alpha -1.64Ccp110 centriolar coiled coil protein 110 -1.65Tnip1 TNFAIP3 interacting protein 1 -1.65Rin3 Ras and Rab interactor 3 -1.65Ift122 intraflagellar transport 122 homolog -1.66Pfkp phosphofructokinase, platelet -1.67Med10 mediator of RNA polymerase II transcription, subunit 10 homolog -1.67Fmo1 flavin containing monooxygenase 1 -1.67Trrap transformation/transcription domain-associated protein -1.67Tom1 target of myb1 homolog -1.67Atp6ap2 ATPase, H+ transporting, lysosomal accessory protein 2 -1.67Pigz phosphatidylinositol glycan anchor biosynthesis, class Z -1.69Mboat2 membrane bound O-acyltransferase domain containing 2 -1.69Pcif1 PDX1 C-terminal inhibiting factor 1 -1.69Zadh1 zinc binding alcohol dehydrogenase, domain containing 1 -1.69Paip2b pol -1.69Rab7 RAB7, member RAS oncogene family -1.69Nrg3 neuregulin 3, transcript variant 1 -1.7Tsc2 tuberous sclerosis 2 -1.7Mfap1b microfibrillar-associated protein 1B -1.71Rpo1-3 RNA polymerase 1-3 -1.71Anapc5 anaphase-promoting complex subunit 5 -1.72Igsf3 immunoglobulin superfamily, member 3 -1.72Cyp2j9 cytochrome P450, family 2, subfamily j, polypeptide 9 -1.72Ift81 intraflagellar transport 81 homolog -1.73Exoc2 exocyst complex component 2 -1.73

Slc35e1 solute carrier family 35, member E1 -1.73Aste1 asteroid homolog 1 -1.73Rbm18 RNA binding motif protein 18 -1.73Ccrk cell cycle related kinase -1.75Dact2 dapper homolog 2, antagonist of beta-catenin -1.75Hprt1 hypoxanthine guanine phosphoribosyl transferase 1 -1.75Tsc22d4 TSC22 domain family 4 -1.75LOC100045887 similar to PTB-associated splicing factor -1.76Kif5a kinesin family member 5A -1.76Crkl v-crk sarcoma virus CT10 oncogene homolog -1.76Khdrbs1 KH domain containing, RNA binding, signal transduction associated 1 -1.76Gnptg N-acetylglucosamine-1-phosphotransferase, gamma subunit -1.762700081O15Rik RIKEN cDNA 2700081O15 gene -1.77Nit1 nitrilase 1 -1.78Ube2o ubiquitin-conjugating enzyme E2O -1.78Med8 mediator of RNA polymerase II transcription, subunit 8 homolog -1.78Mobp myelin-associated oligodendrocytic basic protein -1.78Cecr6 cat eye syndrome chromosome region, candidate 6 homolog -1.79Cdkn2c cyclin-dependent kinase inhibitor 2C -1.79Rims3 regulating synaptic membrane exocytosis 3 -1.79Zfp87 zinc finger protein 87 -1.79Otx1 orthodenticle homolog 1 -1.796430571L13Rik RIKEN cDNA 6430571L13 gene -1.8Hr hairless -1.81Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 -1.83Dcx doublecortin -1.83Obfc2b oligonucleotide/oligosaccharide-binding fold containing 2B -1.84Dusp26 dual specificity phosphatase 26 -1.84Nsmaf neutral sphingomyelinase (N-SMase) activation associated factor -1.84Hist1h2ad histone cluster 1, H2ad -1.85Ivns1abp influenza virus NS1A binding protein -1.87Bmpr1a bone morphogenetic protein receptor, type 1A -1.87Gpc5 glypican 5 -1.88Tardbp TAR DNA binding protein -1.89Sox21 SRY-box containing gene 21 -1.91Krt222 keratin 222 -1.92Tshz3 teashirt zinc finger family member 3 -1.92Fn3k fructosamine 3 kinase -1.92Cacng3 calcium channel, voltage-dependent, gamma subunit 3 -1.93Gpr17 G protein-coupled receptor 17 -1.93Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform -1.94Pcyox1l prenylcysteine oxidase 1 like -1.95Hspa2 heat shock protein 2 -1.95Ociad1 OCIA domain containing 1 -1.96Lmna lamin A -1.98Islr2 immunoglobulin superfamily containing leucine-rich repeat 2 -1.98Mrpl3 mitochondrial ribosomal protein L3 -1.98Rnf6 ring finger protein (C3H2C3 type) 6 -1.99Apcdd1 adenomatosis polyposis coli down-regulated 1 -1.99Decr2 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal -2.01Centb5 centaurin, beta 5 -2.02Lix1 limb expression 1 homolog -2.02Epm2aip1 EPM2A -2.03Ppm1m protein phosphatase 1M -2.03

Fads2 fatty acid desaturase 2 -2.04Rab6 RAB6, member RAS oncogene family -2.05Dctd dCMP deaminase -2.05Mrps7 mitchondrial ribosomal protein S7 -2.06Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A -2.06Klc4 kinesin light chain 4 -2.09Prkcz protein kinase C, zeta -2.09Itm2a integral membrane protein 2A -2.1Prkaa2 protein kinase, AMP-activated, alpha 2 catalytic subunit -2.1Ncald neurocalcin delta -2.11Ccno cyclin O -2.11LOC100047619 similar to solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 -2.118430419L09Rik RIKEN cDNA 8430419L09 gene -2.11Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide -2.12Kif1b kinesin family member 1B -2.12Chac1 ChaC, cation transport regulator-like 1 -2.12Sorbs1 sorbin and SH3 domain containing 1 -2.12Prune prune homolog -2.12Kif21a kinesin family member 21A -2.15Smox spermine oxidase -2.16Hexdc hexosaminidase -2.16Dusp18 dual specificity phosphatase 18 -2.16Galnt1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 -2.17Acpl2 acid phosphatase-like 2 -2.18Slc8a2 solute carrier family 8 -2.2Mrp63 mitochondrial ribosomal protein 63 -2.2Zeb2 zinc finger E-box binding homeobox 2 -2.2Tspan2 tetraspanin 2 -2.2Wnt10a wingless related MMTV integration site 10a -2.21Mfn2 mitofusin 2 -2.22Khdrbs1 KH domain containing, RNA binding, signal transduction associated 1 -2.23Rabl2a RAB, member of RAS oncogene family-like 2A -2.24Cpsf3l cleavage and polyadenylation specific factor 3-like -2.26AI413582 expressed sequence AI413582 -2.27Zfp160 zinc finger protein 160 -2.27Pik3ip1 phosphoinositide-3-kinase interacting protein 1 -2.28Cyp51 cytochrome P450, family 51 -2.28Itpka inositol 1,4,5-trisphosphate 3-kinase A -2.28Zfp213 zinc finger protein 213 -2.29Hagh hydroxyacyl glutathione hydrolase -2.29Mal myelin and lymphocyte protein, T-cell differentiation protein -2.3Cox6a2 cytochrome c oxidase, subunit VI a, polypeptide 2 -2.31Acot7 acyl-CoA thioesterase 7 -2.32Zfp691 zinc finger protein 691 -2.34Tmem8 transmembrane protein 8 (five membrane-spanning domains) -2.34Cd200 CD200 antigen -3.44Npr2 natriuretic peptide receptor 2 -2.36Gldc glycine decarboxylase -2.39Ddit4l DNA-damage-inducible transcript 4-like -2.39Lrrc20 leucine rich repeat containing 20 -2.39Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -2.4Gbp2 guanylate nucleotide binding protein 2 -2.4Atf4 activating transcription factor 4 -2.42Id4 inhibitor of DNA binding 4 -2.43

Trak2 trafficking protein, kinesin binding 2 -2.43Mef2c myocyte enhancer factor 2C -2.43Med23 mediator complex subunit 23 -2.44Cxcl12 chemokine (C-X-C motif) ligand 12, transcript variant 3 -2.44Itfg2 integrin alpha FG-GAP repeat containing 2 -2.44LOC100047934 hypothetical protein LOC100047934 -2.45Ttc17 tetratricopeptide repeat domain 17 -2.45Bok BCL2-related ovarian killer -2.47Phlda3 pleckstrin homology-like domain, family A, member 3 -2.47Actb actin, beta, cytoplasmic -2.49Ivd isovaleryl coenzyme A dehydrogenase -2.49Ccndbp1 cyclin D-type binding-protein 1 -2.51Ndufs2 NADH dehydrogenase (ubiquinone) Fe-S protein 2 -2.51Bex4 brain expressed gene 4 -2.51Axud1 AXIN1 up-regulated 1 -2.51LOC100045439 similar to testis-specific adriamycin sensitivity protein -2.52Pex19 peroxisome biogenesis factor 19 -2.53Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1 -2.54Psmd9 proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 -2.55Pdlim2 PDZ and LIM domain 2 -2.55Ank2 ankyrin 2, brain -2.56Ccnd1 cyclin D1 -2.64Ehd3 EH-domain containing 3 -2.57Fbxl18 F-box and leucine-rich repeat protein 18 -2.61Scnm1 sodium channel modifier 1, transcript variant 1 -2.62Ccrn4l CCR4 carbon catabolite repression 4-like -2.65Extl2 exostoses (multiple)-like 2 -2.66Cxcl12 chemokine (C-X-C motif) ligand 12, transcript variant 1 -2.67Rgl1 ral guanine nucleotide dissociation stimulator,-like 1 -2.67Sytl2 synaptotagmin-like 2 -2.7Ttyh2 tweety homolog 2 -2.72Mog myelin oligodendrocyte glycoprotein -2.73Anxa3 annexin A3 -2.73Sh3gl2 SH3-domain GRB2-like 2 -2.74E430002G05Rik RIKEN cDNA E430002G05 gene -2.75Scarf2 scavenger receptor class F, member 2 -2.76Dennd6b DENN/MADD domain containing 6B -2.78Ivns1abp influenza virus NS1A binding protein -2.78Sv2a synaptic vesicle glycoprotein 2 a -2.78C230078M08Rik RIKEN cDNA C230078M08 gene -2.79LOC100043257 similar to RNA binding motif protein 3 -2.8Hrbl HIV-1 Rev binding protein-like -2.82Traf7 Tnf receptor-associated factor 7 -2.82Ttyh2 tweety homolog 2 (Drosophila) -2.83Abcd3 ATP-binding cassette, sub-family D -2.83Trem2 triggering receptor expressed on myeloid cells 2 -2.84Socs6 suppressor of cytokine signaling 6 -2.84Actl6b actin-like 6B -2.85Usp21 ubiquitin specific peptidase 21 -2.86Caskin1 CASK interacting protein 1 -2.87Mto1 mitochondrial translation optimization 1 homolog -2.87Fbxl18 F-box and leucine-rich repeat protein 18 -2.99Tfrc transferrin receptor -2.99Ifit3 interferon-induced protein with tetratricopeptide repeats 3 -3.01

Cckbr cholecystokinin B receptor -3.06Rnf41 ring finger protein 41 -3.06LOC100041290 hypothetical protein LOC100041290 -3.07Bach2 BTB and CNC homology 2 -3.08Pdlim2 PDZ and LIM domain 2 -3.09Hebp1 heme binding protein 1 -3.1Rnd3 Rho family GTPase 3 -3.1H2-T23 histocompatibility 2, T region locus 23 -3.14Zfp367 zinc finger protein 367 -3.19Anxa3 annexin A3 -3.21Syt17 synaptotagmin XVII -3.21Bok Bcl-2-related ovarian killer protein -3.21Fbxo44 F-box protein 44 -3.23Med8 mediator of RNA polymerase II transcription, subunit 8 homolog -3.24Pdlim2 PDZ and LIM domain 2 -3.24Dusp7 dual specificity phosphatase 7 -3.25Zfp101 zinc finger protein 101 -3.25Fam82a2 family with sequence similarity 82, member A2 -3.3Them4 thioesterase superfamily member 4 -3.3Hexdc hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing -3.36AI316807 expressed sequence AI316807 -3.41Rasl10a RAS-like, family 10, member A -3.41Arl6ip1 ADP-ribosylation factor-like 6 interacting protein 1 -3.42Mog myelin oligodendrocyte glycoprotein -3.47Tprkb Tp53rk binding protein -3.47Yipf4 Yip1 domain family, member 4 -3.52Ppox protoporphyrinogen oxidase -3.56Tmc7 transmembrane channel-like gene family 7 -3.63Chd5 chromodomain helicase DNA binding protein 5 -3.67Msr2 macrophage scavenger receptor 2 -3.7Tmod1 tropomodulin 1 -3.72Pik3r4 phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 -3.74Mrps7 mitchondrial ribosomal protein S7 -3.76Rshl2a radial spokehead-like 2A -3.79Abcc10 ATP-binding cassette, sub-family C (CFTR/MRP), member 10, transcript variant mrp7B -3.79Vangl2 vang-like 2 (van gogh, Drosophila) -3.8Dhrsx dehydrogenase/reductase (SDR family) X chromosome -3.81Ddr1 discoidin domain receptor family, member 1 -3.81Ush2a Usher syndrome 2A (autosomal recessive, mild) homolog (human) -3.82Slc35a5 solute carrier family 35, member A5 -3.89Zfp157 zinc finger protein 157 -3.95Tfrc transferrin receptor -3.97Rasgrp1 RAS guanyl releasing protein 1 -4.1Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -4.22 Fastkd3 FAST kinase domains 3 -4.22Arsj arylsulfatase J -4.27Aldh1a1 aldehyde dehydrogenase family 1, subfamily A1 -4.28Usf1 upstream transcription factor 1 -4.41Sbf1 SET binding factor 1 -4.43Med23 mediator complex subunit 23 -4.45Actl6b actin-like 6B -4.6LOC100046039 similar to histone deacetylase HD1 -4.66Cort cortistatin -4.68BC017643 cDNA sequence BC017643 -4.69

Atp2c1 ATPase, Ca++-sequestering -4.69Smco3 single-pass membrane protein with coiled-coil domains 3 -4.74Spink8 serine peptidase inhibitor, Kazal type 8 -4.75Lars2 leucyl-tRNA synthetase, mitochondrial -4.76Agxt2l1 alanine-glyoxylate aminotransferase 2-like 1 -4.93Arl5a ADP-ribosylation factor-like 5A -5.68Arhgef19 Rho guanine nucleotide exchange factor -5.06Klhl21 kelch-like 21 -5.17Snapc3 small nuclear RNA activating complex, polypeptide 3 -5.23Arl5a ADP-ribosylation factor-like 5A -5.24Slc25a18 solute carrier family 25 (mitochondrial carrier), member 18 -5.26Fgfr1op2 FGFR1 oncogene partner 2 -5.26Atpbd1b ATP binding domain 1 family, member B -5.27Tagap T-cell activation Rho GTPase-activating protein -5.29Tsc2 tuberous sclerosis 2 -5.39LOC433801 similar to RIKEN cDNA 6330416L07 gene -5.47Lgals3bp lectin, galactoside-binding, soluble, 3 binding protein -5.5Mod1 malic enzyme, supernatant -5.53Cort cortistatin -5.53Agxt2l1 alanine-glyoxylate aminotransferase 2-like 1 -5.6LOC545056 ubiquitin-conjugating enzyme E2, J2 homolog pseudogene -5.66Rab3b RAB3B, member RAS oncogene family -5.89Lrrc57 leucine rich repeat containing 57 -6.1Epm2aip1 EPM2A (laforin) interacting protein 1 -6.16Atp8a1 ATPase, aminophospholipid transporter -6.27Rpl29 ribosomal protein L29 -6.3Ccp110 centriolar coiled coil protein 110 -6.31Bsdc1 BSD domain containing 1 -6.39Agpat5 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) -7.17Pex19 peroxisome biogenesis factor 19 -7.43Rps15a ribosomal protein S15a -7.65Myoc myocilin -7.68Ccl27 chemokine (C-C motif) ligand 27, transcript variant 1 -8.43Zfp68 zinc finger protein 68 -8.57Actb actin, beta, cytoplasmic -8.86Ccl27 chemokine (C-C motif) ligand 27, transcript variant 2 -9.34Uap1 UDP-N-acetylglucosamine pyrophosphorylase 1 -9.4Zfp365 zinc finger protein 365 -9.72Clstn1 calsyntenin 1, transcript variant 1 -10.22Mgst1 microsomal glutathione S-transferase 1 -10.42Ccng2 cyclin G2 -10.59Taf6 TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor -10.87Ccl21b chemokine (C-C motif) ligand 21b -11.55Cfap69 cilia and flagella associated protein 69 -11.79Actl6b actin-like 6B -12.37B3galt6 UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 -14.45Zfp68 zinc finger protein 68 -15.07Fcer1g Fc receptor, IgE, high affinity I, gamma polypeptide -22.31

Table S3. Gene transcripts significantly and differentially regulated in T1R3KO hypothalamus compared to WT control cortex. For each significantly-regulated transcript the Official Gene Symbol, transcript description and expression Z ratio, T1R3KO versus WT are represented.

Gene Symbol Description Z ratio T1R3KO:WTH2-Bl histocompatibility 2, blastocyst 16.47Tmem87a transmembrane protein 87A 12.85Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog 7.99Cap1 CAP, adenylate cyclase-associated protein 1 7.75Npvf neuropeptide VF precursor 7.52Scp2 sterol carrier protein 2, liver 6.57Nubp2 nucleotide binding protein 2 6.47Pgk1 phosphoglycerate kinase 1 6.24Rbm45 RNA binding motif protein 45 6.12Rgs5 regulator of G-protein signaling 5 5.99Ndufb10 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 5.38Gnb1 guanine nucleotide binding protein (G protein), beta 1 5.27Wars2 tryptophanyl tRNA synthetase 2 (mitochondrial) 4.99Fcgr3 Fc receptor, IgG, low affinity III 4.69Hyi hydroxypyruvate isomerase homolog 4.51Hdhd3 haloacid dehalogenase-like hydrolase domain containing 3 4.5Snapc3 small nuclear RNA activating complex, polypeptide 3 4.43Mns1 meiosis-specific nuclear structural protein 1 4.41Vps39 vacuolar protein sorting 39 4.38Vps39 vacuolar protein sorting 39 4.38Frmd3 FERM domain containing 3, transcript variant 1 4.35Itgb3bp integrin beta 3 binding protein (beta3-endonexin) 4.28Nubp2 nucleotide binding protein 2 4.24Apoc1 apolipoprotein C-I 4.06Dnaic1 dynein, axonemal, intermediate chain 1 3.94Cox18 COX18 cytochrome c oxidase assembly homolog 3.83Snx30 sorting nexin family member 30 3.76Alad aminolevulinate, delta-, dehydratase 3.59Rec8 REC8 homolog 3.58Lmo3 LIM domain only 3 3.55Habp4 hyaluronic acid binding protein 4 3.45Hmgn2 high mobility group nucleosomal binding domain 2 3.45Coq5 coenzyme Q5 homolog, methyltransferase 3.44Apoc1 apolipoprotein C-I 3.43Gpr19 G protein-coupled receptor 19 3.38Gins4 GINS complex subunit 4 (Sld5 homolog) 3.34Dnajb4 DnaJ (Hsp40) homolog, subfamily B, member 4 3.34Vars2 valyl-tRNA synthetase 2, mitochondrial 3.33Glipr2 GLI pathogenesis-related 2 3.23Asah3l N-acylsphingosine amidohydrolase 3-like 3.22Mrps10 mitochondrial ribosomal protein S10 3.22Ankrd56 ankyrin repeat domain 56 3.212310002B06Rik RIKEN cDNA 2310002B06 gene 3.21Ppt1 palmitoyl-protein thioesterase 1 3.2Clec16a C-type lectin domain family 16, member A 3.16Vip vasoactive intestinal polypeptide 3.15Dtna dystrobrevin alpha 3.09

Lck lymphocyte protein tyrosine kinase 3.04Lbh limb-bud and heart 3.01Eno1 enolase 1, alpha non-neuron 2.98Extl1 exostoses (multiple)-like 1 2.97Pfdn2 prefoldin 2 2.93Ebna1bp2 EBNA1 binding protein 2 2.93Chchd8 coiled-coil-helix-coiled-coil-helix domain containing 8 2.89Adamts4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 2.81Gfer growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration) 2.79Vps33a vacuolar protein sorting 33A 2.74Mrps10 mitochondrial ribosomal protein S10 2.72Coq5 coenzyme Q5 homolog, methyltransferase 2.72Nmu neuromedin U 2.67Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 2.67Tnfrsf21 tumor necrosis factor receptor superfamily, member 21 2.66Sorl1 sortilin-related receptor, LDLR class A repeats-containing 2.64Fgf1 fibroblast growth factor 1 2.64Nol4 nucleolar protein 4 2.63Gng10 guanine nucleotide binding protein (G protein), gamma 10 (Gng10) 2.63LOC100044159 hypothetical protein LOC100044159 2.6Coq5 coenzyme Q5 homolog, methyltransferase 2.58Pi16 peptidase inhibitor 16 2.56Ttll12 tubulin tyrosine ligase-like family, member 12 2.5Rnaseh2c ribonuclease H2, subunit C 2.5Clic6 chloride intracellular channel 6 2.48Sepn1 selenoprotein N, 1 2.48Crhbp corticotropin releasing hormone binding protein 2.43Haus8 4HAUS augmin-like complex, subunit 8 2.42Gstm6 glutathione S-transferase, mu 6 2.39Ngb neuroglobin 2.39Kptn kaptin 2.37Gstm6 glutathione S-transferase, mu 6 2.36B4galt3 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 2.36Gm129 gene model 129, 2.324930539E08Rik RIKEN cDNA 4930539E08 gene 2.31Vgf VGF nerve growth factor inducible 2.3Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase 2.28Arhgap27 Rho GTPase activating protein 27 2.28Pvrl3 poliovirus receptor-related 3, transcript variant beta 2.28

GnasGNAS (guanine nucleotide binding protein, alpha stimulating) complex locus, transcript variant 9, transcrib 2.26

Ccdc28a coiled-coil domain containing 28A 2.25Tctex1d2 Tctex1 domain containing 2 2.24Amn amnionless 2.24Zxdc ZXD family zinc finger C 2.22Fgf1 fibroblast growth factor 1 2.21Ganc glucosidase, alpha; neutral C 2.25133401N09Rik RIKEN cDNA 5133401N09 gene 2.2Lix1 limb expression 1 homolog (chicken) 2.2Poll polymerase (DNA directed), lambda (Poll) 2.21110017D15Rik RIKEN cDNA 1110017D15 gene 2.19Mycbpap Mycbp associated protein 2.18Immp2l IMP2 inner mitochondrial membrane peptidase-like 2.18Rpl22 ribosomal protein L22 2.16Rwdd3 RWD domain containing 3 2.15

Vti1a vesicle transport through interaction with t-SNAREs homolog 1A 2.13Pcsk2 proprotein convertase subtilisin/kexin type 2 2.12C4b complement component 4B (Childo blood group) 2.11Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor 1 2.07Clip1 CAP-GLY domain containing linker protein 1 2.07Dtna dystrobrevin alpha 2.07Slc5a5 solute carrier family 5 (sodium iodide symporter), member 5 2.06Sdhc succinate dehydrogenase complex, subunit C, integral membrane protein 2.05A930025D01Rik RIKEN cDNA A930025D01 gene 2.04Ddit3 DNA-damage inducible transcript 3 2.04Atp13a4 ATPase type 13A4 2.03As3mt arsenic (+3 oxidation state) methyltransferase 2.03Pex6 peroxisomal biogenesis factor 6 2.03Creb3 cAMP responsive element binding protein 3 2.02Unc45a unc-45 homolog A 1.99Camk2d calcium/calmodulin-dependent protein kinase II, delta 1.97Ctnnd2 catenin (cadherin associated protein), delta 2 1.96Cenpl centromere protein L 1.96Ctps cytidine 5'-triphosphate synthase 1.96Rims1 regulating synaptic membrane exocytosis 1 1.95Pacrg Park2 co-regulated 1.95Dclk3 doublecortin-like kinase 3 1.95Phactr1 phosphatase and actin regulator 1 1.95Pvalb parvalbumin 1.95Pdia4 protein disulfide isomerase associated 4 1.94Faim2 Fas apoptotic inhibitory molecule 2 1.93D4Ertd22e DNA segment, Chr 4, ERATO Doi 22, expressed 1.931110017D15Rik RIKEN cDNA 1110017D15 gene 1.93Irak2 interleukin-1 receptor-associated kinase 2 1.93Ccrk cell cycle related kinase 1.93Phactr4 phosphatase and actin regulator 4 1.91Ap4m1 adaptor-related protein complex AP-4, mu 1 1.91Shf Src homology 2 domain containing F 1.9Slc6a15 solute carrier family 6 (neurotransmitter transporter), member 15 1.9Dhps deoxyhypusine synthase 1.89Synj2bp synaptojanin 2 binding protein 1.882610524H06Rik RIKEN cDNA 2610524H06 gene 1.87Rimkla ribosomal modification protein rimK-like family member A 1.86Ebna1bp2 EBNA1 binding protein 2 1.86Preb prolactin regulatory element binding 1.85Abcg1 ATP-binding cassette, sub-family G (WHITE), member 1 1.85Tmem62 transmembrane protein 62 1.85Lphn3 latrophilin 3 1.84Rabggta Rab geranylgeranyl transferase, a subunit 1.83Ddx51 DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 1.83Tarsl2 threonyl-tRNA synthetase-like 2 1.83Rtn2 reticulon 2 (Z-band associated protein), transcript variant B 1.8Ethe1 ethylmalonic encephalopathy 1 1.79Donson downstream neighbor of SON 1.78Gmpr guanosine monophosphate reductase 1.78Habp4 hyaluronic acid binding protein 4 1.78Eif3s1 eukaryotic translation initiation factor 3, subunit 1 alpha 1.78 Ncs1 neuronal calcium sensor 1 1.77Il17d interleukin 17D 1.76

Mcph1 microcephaly, primary autosomal recessive 1 1.76Fgf12 fibroblast growth factor 12 1.75Peg10 paternally expressed 10 1.742610042L04Rik RIKEN cDNA 2610042L04 gene 1.74Tjap1 tight junction associated protein 1 1.74Nrip3 nuclear receptor interacting protein 3 1.74Ttll1 tubulin tyrosine ligase-like 1 1.73Dbpht2 DNA binding protein with his-thr domain 1.73Gfm1 G elongation factor, mitochondrial 1 1.72Srprb signal recognition particle receptor, B subunit 1.72Nudc nuclear distribution gene C homolog 1.72Rftn1 raftlin lipid raft linker 1 1.72Rps6ka1 ribosomal protein S6 kinase polypeptide 1 1.72Capza1 capping protein (actin filament) muscle Z-line, alpha 1 1.72Mrpl3 mitochondrial ribosomal protein L3 1.71Pnpo pyridoxine 5'-phosphate oxidase 1.71Acy1 aminoacylase 1 1.71Gpr89 G protein-coupled receptor 89 1.7Mapk3 mitogen activated protein kinase 3 1.69Ubxd1 UBX domain containing 1 1.68Chchd5 coiled-coil-helix-coiled-coil-helix domain containing 5 1.68Fbxo6 F-box protein 6 1.67Krtcap3 keratinocyte associated protein 3 1.66Aurkaip1 aurora kinase A interacting protein 1 1.66Cpne9 copine family member IX 1.66Adam22 a disintegrin and metallopeptidase domain 22 1.65Slc7a3 solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 1.65Mrpl17 mitochondrial ribosomal protein L17 1.64H1fx H1 histone family, member X 1.64Tprgl transformation related protein 63 regulated like 1.63Lsm2 LSM2 homolog, U6 small nuclear RNA associated 1.63Tex264 testis expressed gene 264 1.62Ngb neuroglobin 1.62Ars2 arsenate resistance protein 2 1.61Pvrl3 poliovirus receptor-related 3 1.6LOC100048083 similar to Tect2 1.6Sumf2 sulfatase modifying factor 2 1.6Gpx3 glutathione peroxidase 3 1.6Lgals3 lectin, galactose binding, soluble 3 1.6Jazf1 JAZF zinc finger 1 1.6Hbegf heparin-binding EGF-like growth factor 1.59Dync1i1 dynein cytoplasmic 1 intermediate chain 1 1.59Hnrpc heterogeneous nuclear ribonucleoprotein C, transcript variant 1 1.59Zfp612 zinc finger protein 612 1.59Tex264 testis expressed gene 264 1.59Stmn1 stathmin 1 1.58Car11 carbonic anhydrase 11 1.58Tmem143 transmembrane protein 143 1.58Peli3 pellino 3 1.58Trim11 tripartite motif-containing 11 1.57Garnl4 GTPase activating RANGAP domain-like 4 1.57Ttc39a tetratricopeptide repeat domain 39A 1.57Nptx2 neuronal pentraxin 2 1.56Eid2 EP300 interacting inhibitor of differentiation 2 1.56

Tbc1d5 TBC1 domain family, member 5 1.56Mad2l2 MAD2 mitotic arrest deficient-like 2 1.56Zfp398 zinc finger protein 398 1.56Dzip1l DAZ interacting protein 1-like 1.55Pcsk2 proprotein convertase subtilisin/kexin type 2 1.54Adgrb3 adhesion G protein-coupled receptor B3 1.54Cog6 component of oligomeric golgi complex 6 1.532610024G14Rik RIKEN cDNA 2610024G14 gene 1.53Tsr2 TSR2, 20S rRNA accumulation, homolog 1.53Zfand2b zinc finger, AN1 type domain 2B 1.53Tmem186 transmembrane protein 186 1.52Dvl1 dishevelled, dsh homolog 1 (Drosophila) 1.52Sppl3 signal peptide peptidase 3 1.52Tmco3 transmembrane and coiled-coil domains 3 1.51Srm spermidine synthase 1.51Cds2 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 1.51Copg coatomer protein complex, subunit gamma, transcript variant 2 1.51Hn1 hematological and neurological expressed sequence 1 1.51Zfp704 zinc finger protein 704 1.51Prkab1 protein kinase, AMP-activated, beta 1 non-catalytic subunit 1.5Poldip3 polymerase (DNA-directed), delta interacting protein 3 1.5Traf7 Tnf receptor-associated factor 7 -1.51Arid5b AT rich interactive domain 5B -1.51Dusp26 dual specificity phosphatase 26 -1.51Enkur enkurin, TRPC channel interacting protein -1.52Hs3st1 heparan sulfate (glucosamine) 3-O-sulfotransferase 1 -1.53Echdc2 enoyl Coenzyme A hydratase domain containing 2 -1.53Evi2a ecotropic viral integration site 2a -1.54Rell1 RELT-like 1 -1.55Them2 thioesterase superfamily member 2 -1.55Usp10 ubiquitin specific peptidase 10 -1.56Prei3 preimplantation protein 3 -1.56Nudt4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 -1.56Hspa2 heat shock protein 2, transcript variant 2 -1.57Emp2 epithelial membrane protein 2 -1.57Usp21 ubiquitin specific peptidase 21 -1.581810035L17Rik RIKEN cDNA 1810035L17 gene -1.59Scarf2 scavenger receptor class F, member 2 -1.62Dnaja2 DnaJ (Hsp40) homolog, subfamily A, member 2 -1.62Timp3 tissue inhibitor of metalloproteinase 3 -1.62Trib2 tribbles homolog 2 (Drosophila) -1.63Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -1.64Hexdc hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing -1.64Peci peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase -1.65Hist1h2af histone cluster 1, H2af -1.66Sypl synaptophysin-like protein -1.66Tcf4 transcription factor 4 -1.67Actl6b actin-like 6B -1.68Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog -1.69Dpysl4 dihydropyrimidinase-like 4 -1.69Mapk4 mitogen-activated protein kinase 4 -1.69Fastkd5 FAST kinase domains 5 -1.7Dctd dCMP deaminase -1.7Cox6c cytochrome c oxidase, subunit VIc -1.71

Ttyh2 tweety homolog 2 (Drosophila) -1.71Myh9 myosin, heavy polypeptide 9, non-muscle, transcript variant 1 -1.72Lass2 LAG1 homolog, ceramide synthase 2 -1.73Litaf LPS-induced TN factor -1.73Cml4 camello-like 4 -1.73Arpc1b actin related protein 2/3 complex, subunit 1B -1.74Slc12a2 solute carrier family 12, member 2 -1.74Adam22 a disintegrin and metallopeptidase domain 22 -1.74Pcca propionyl-Coenzyme A carboxylase, alpha polypeptide -1.75Lamp2 lysosomal membrane glycoprotein 2 -1.75Armc9 armadillo repeat containing 9 -1.75Sirt2 sirtuin 2 (silent mating type information regulation 2, homolog) 2 -1.76Rras2 related RAS viral (r-ras) oncogene homolog 2 -1.76Itm2b integral membrane protein 2B -1.77Evi2a ecotropic viral integration site 2a -1.77Swap70 SWA-70 protein -1.772610002J02Rik RIKEN cDNA 2610002J02 gene -1.77Lass2 LAG1 homolog, ceramide synthase 2 -1.77Hfe hemochromatosis -1.77Gja1 gap junction membrane channel protein alpha 1 -1.79Unc5b unc-5 homolog B -1.8Mrpl50 mitochondrial ribosomal protein L50 -1.8Bzw1 basic leucine zipper and W2 domains 1 -1.81Lyrm5 LYR motif containing 5 -1.82Ufc1 ubiquitin-fold modifier conjugating enzyme 1 -1.82Mag myelin-associated glycoprotein -1.82Extl2 exostoses (multiple)-like 2 -1.82Fa2h fatty acid 2-hydroxylase -1.83Ttc17 tetratricopeptide repeat domain 17 -1.84Lims2 LIM and senescent cell antigen like domains 2 -1.84LOC100044779 similar to prothymosin alpha -1.85Rgs7 regulator of G protein signaling 7 -1.85Slc12a2 solute carrier family 12, member 2 -1.86Wscd1 WSC domain containing 1 -1.87Pldn pallidin -1.88S100b S100 protein, beta polypeptide, neural -1.9Slc4a2 solute carrier family 4 (anion exchanger), member 2 -1.92Purb purine rich element binding protein B -1.94Nhsl1 NHS-like 1 -1.96Cct5 chaperonin subunit 5 -1.98Rgma RGM domain family, member A -1.98Slc16a6 solute carrier family 16 (monocarboxylic acid transporters), member 6, transcript variant 2 -1.99Rrp1 ribosomal RNA processing 1 homolog -1.99Acaa2 acetyl-Coenzyme A acyltransferase 2 -1.99Ehd3 EH-domain containing 3 -2Riok1 RIO kinase 1 -2.02Gosr2 golgi SNAP receptor complex member 2 -2.03Ifit3 interferon-induced protein with tetratricopeptide repeats 3 -2.04Car14 carbonic anhydrase 14 -2.04Nit1 nitrilase 1 -2.04Il11ra1 interleukin 11 receptor, alpha chain 1 -2.05Clic4 chloride intracellular channel 4 (mitochondrial) -2.05Bcas1 breast carcinoma amplified sequence 1 -2.05Necap2 NECAP endocytosis associated 2 -2.06

Ank2 ankyrin 2, brain -2.07Plekhg3 pleckstrin homology domain containing, family G -2.08Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 -2.08Lamp2 lysosomal-associated membrane protein 2 -2.09Clcn7 chloride channel 7 -2.1Ccrn4l CCR4 carbon catabolite repression 4-like -2.11Ivd isovaleryl coenzyme A dehydrogenase -2.12Copa coatomer protein complex subunit alpha -2.121700084C01Rik RIKEN cDNA 1700084C01 gene -2.165Emc7 ER membrane protein complex subunit 7 -2.16Cryzl1 crystallin, zeta (quinone reductase)-like 1 -2.16Mal myelin and lymphocyte protein, T-cell differentiation protein -2.16Acaa2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) -2.22Hexdc hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing -2.22Id4 inhibitor of DNA binding 4 -2.24Tmod1 tropomodulin 1 -2.252310016C16Rik RIKEN cDNA 2310016C16 gene -3.24Dci dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenyme A isomerase) -2.26Cd59a CD59a antigen -2.28Zfp36l1 zinc finger protein 36, C3H type-like 1 -2.29Rnf41 ring finger protein 41 -2.29Med8 mediator of RNA polymerase II transcription, subunit 8 homolog -2.31Them4 thioesterase superfamily member 4 -2.32Ociad1 OCIA domain containing 1 -2.35S100a10 S100 calcium binding protein A10 (calpactin) -2.35Mobp myelin-associated oligodendrocytic basic protein -2.39Mog myelin oligodendrocyte glycoprotein -2.4H2-M3 histocompatibility 2, M region locus 3 -2.41Rab3c RAB3C, member RAS oncogene family -2.42Med23 mediator complex subunit 23 -2.43Cpsf3l cleavage and polyadenylation specific factor 3-like -2.43Dab2 disabled homolog 2 (Drosophila), transcript variant 1 -2.45Ppox protoporphyrinogen oxidase -2.45Arl6ip1 ADP-ribosylation factor-like 6 interacting protein 1 -2.46Iigp2 interferon inducible GTPase 2 -2.46Gpr17 G protein-coupled receptor 17 -2.5Med8 mediator of RNA polymerase II transcription, subunit 8 homolog -2.51Grb14 growth factor receptor bound protein 14 -2.52Pllp plasma membrane proteolipid -2.53Dusp7 dual specificity phosphatase 7 -2.53B2m beta-2 microglobulin -2.55Gpr177 G protein-coupled receptor 177 -2.55Darc Duffy blood group, chemokine receptor -2.58Ptprb protein tyrosine phosphatase, receptor type, B -2.59B2m beta-2 microglobulin -2.6Hist1h1c histone cluster 1, H1c -2.66Gnptg N-acetylglucosamine-1-phosphotransferase, gamma subunit -2.68E130012A19Rik RIKEN cDNA E130012A19 gene -2.69Rbmx RNA binding motif protein, X chromosome -2.71Rps3a ribosomal protein S3a -2.76Rnf41 ring finger protein 41 -2.781700029J07Rik RIKEN cDNA 1700029J07 gene -2.78Pgm2 phosphoglucomutase 2 -2.78Slc7a11 solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 -2.79

Caskin1 CASK interacting protein 1 -2.79Pdlim2 PDZ and LIM domain 2 -2.81Agtrap angiotensin II, type I receptor-associated protein -2.88Abcd3 ATP-binding cassette, sub-family D -2.88Sparcl1 SPARC-like 1 -2.89Spsb3 splA/ryanodine receptor domain and SOCS box containing 3 -2.9Gbp2 guanylate nucleotide binding protein 2 -2.9Cbwd1 COBW domain containing 1 -2.91Fbxo44 F-box protein 44 -2.93AI316807 expressed sequence AI316807 -2.94Rabl2a RAB, member of RAS oncogene family-like 2A -2.95Pdlim2 PDZ and LIM domain 2 -2.96Anxa2 annexin A2 -2.97Trem2 triggering receptor expressed on myeloid cells 2 -2.98Pdlim2 PDZ and LIM domain 2 -2.98Bid BH3 interacting domain death agonist -2.99Pkd2l1 polycystic kidney disease 2-like 1 -3.02Elavl4 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D), transcript variant 1 -3.03LOC100047749 similar to cAMP-specific cyclic nucleotide phosphodiesterase PDE8; MMPDE8 -3.03Epm2aip1 EPM2A (laforin) interacting protein 1 -3.07Bckdhb branched chain ketoacid dehydrogenase E1, beta polypeptide -3.08Gnptg N-acetylglucosamine-1-phosphotransferase, gamma subunit -3.11Yipf4 Yip1 domain family, member 4 -3.12Hist2h2be histone cluster 2, H2be -3.14Slc35a5 solute carrier family 35, member A5 -3.15Tfrc transferrin receptor -3.15LOC100044190 hypothetical protein LOC100044190 -3.18Pex19 peroxisome biogenesis factor 19 -3.22Ush2a Usher syndrome 2A -3.24Tfrc transferrin receptor -3.28Ccndbp1 cyclin D-type binding-protein 1 -3.29Sbf1 SET binding factor 1 -3.31Lgals3bp lectin, galactoside-binding, soluble, 3 binding protein -3.32Tceanc2 transcription elongation factor A (SII) N-terminal and central domain containing 2 -3.34Tspan2 tetraspanin 2 -3.36Edg2 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 -3.42Mrps7 mitchondrial ribosomal protein S7 -3.42Usf1 upstream transcription factor 1 -3.43S100a11 S100 calcium binding protein A11 -3.44LOC100046039 similar to histone deacetylase HD1 -3.51Ddr1 discoidin domain receptor family, member 1 -3.55Tsc2 tuberous sclerosis 2 -3.55Zeb2 zinc finger E-box binding homeobox 2 -3.572810432L12Rik RIKEN cDNA 2810432L12 gene -3.6Pla2g7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) -3.61Ptp4a2 protein tyrosine phosphatase 4a2 -3.63Samd9l sterile alpha motif domain containing 9-like, transcript variant 1 -3.69Pebp1 phosphatidylethanolamine binding protein 1 -3.71Arl5a ADP-ribosylation factor-like 5A -3.78Rab3b RAB3B, member RAS oncogene family -3.8Ifi27 interferon, alpha-inducible protein 27 -3.81Slc38a2 solute carrier family 38, member 2 -3.84Cldn11 claudin 11 -3.88Bgn biglycan -3.9

Atpbd1b ATP binding domain 1 family, member B -3.92Ppic peptidylprolyl isomerase C -3.99Arhgap29 Rho GTPase activating protein 29 -4Xkr8 X Kell blood group precursor related family member 8 homolog -4.04Lars2 leucyl-tRNA synthetase, mitochondrial -4.17Snapc3 small nuclear RNA activating complex, polypeptide 3 -4.27Olfml3 olfactomedin-like 3 -4.28Iap intracisternal A particles -4.37Arl5a ADP-ribosylation factor-like 5A -4.76Pik3r4 phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 -4.64Arhgef19 Rho guanine nucleotide exchange factor (GEF) 19 -4.67Mal myelin and lymphocyte protein, T-cell differentiation protein -4.69Srgn serglycin -4.76BC017643 cDNA sequence BC017643 -4.77H2-T23 histocompatibility 2, T region locus 23 -4.79Vangl2 vang-like 2 (van gogh, Drosophila) -4.79Klhl21 kelch-like 21 -4.97Fgfr1op2 FGFR1 oncogene partner 2 -5Rshl2a radial spokehead-like 2A -5.01Lyzs lysozyme -5.04Mod1 malic enzyme, supernatant -5.09Fam82a2 family with sequence similarity 82, member A2 -5.11Atp2c1 ATPase, Ca++-sequestering -5.17Anxa3 annexin A3 -5.2Dhrsx dehydrogenase/reductase (SDR family) X chromosome -5.2Anxa3 annexin A3 -5.42Aldh1a1 aldehyde dehydrogenase family 1, subfamily A1 -5.49Lrrc57 leucine rich repeat containing 57 -5.49LOC545056 ubiquitin-conjugating enzyme E2, J2 homolog pseudogene -5.63Bsdc1 BSD domain containing 1 -5.7Smco3 single-pass membrane protein with coiled-coil domains 3 -5.73Epm2aip1 EPM2A (laforin) interacting protein 1 -5.92Ccl27 chemokine (C-C motif) ligand 27 , transcript variant 1 -5.93Pex19 peroxisome biogenesis factor 19 -6.05Ccp110 centriolar coiled coil protein 110 -6.25Lyz lysozyme -6.26Hbb-b1 hemoglobin, beta adult major chain -6.38Gjb2 gap junction protein, beta 2 -6.42Oasl2 2'-5' oligoadenylate synthetase-like 2 -6.42Thbd thrombomodulin -7.23Nupr1 nuclear protein 1 -7.83Myoc myocilin -7.87Zfp365 zinc finger protein 365 -8.04Ccl27 chemokine (C-C motif) ligand 27, transcript variant 2 -8.25Uap1 UDP-N-acetylglucosamine pyrophosphorylase 1 -8.62Zfp68 zinc finger protein 68 -9.01Clstn1 calsyntenin 1, transcript variant 1 -9.86Rps15a ribosomal protein S15a -10.02Taf6 TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor -10.15Ccng2 cyclin G2 -11.1Actl6b actin-like 6B -11.37LOC433801 similar to RIKEN cDNA 6330416L07 gene -11.52Cfap69 cilia and flagella associated protein 69 -12.71Zfp68 zinc finger protein 68 -13.62

B3galt6 UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 -15.07Gramd4 GRAM domain containing 4 -18.99

Table S4. Venn diagram-based dissection of T1R3KO-specific transcriptomic expression patterns between cortical, hippocampal and hypothalamic tissues. For each identified gene transcript (significantly and differentially regulated between T1R3KO and WT mice) the Official Gene Symbol is given and the Z ratio of transcript expression (T1R3KO:WT) is given.

Gene Symbol Cortex Hippocampus HypothalamusTmem87a 9.14 11.09 12.85Kras 6.13 8.05 7.99Cap1 6.84 7.1 7.75Ndufb10 6.01 7.55 5.38Nubp2 5.54 6.83 6.47Rgs5 6.25 6.04 5.99Fcgr3 6.68 6.48 4.69Hyi 5.11 7.08 4.51Rbm45 4.44 4.22 6.12Scp2 3.19 4.93 6.57Itgb3bp 4.7 5.05 4.28Wars2 4.4 4.27 4.99Cox18 4.41 5.27 3.832310002B06Rik 5.41 4.34 3.21Gnb1 3.93 2.79 5.27Alad 4.13 4.09 3.59Dnaic1 3.53 3.92 3.94Clec16a 3.7 4.26 3.16Vars2 3.66 4.11 3.33Hdhd3 2.6 3.96 4.5Hmgn2 3.81 3.75 3.45Adamts4 3.59 4.6 2.81Snapc3 3.31 2.61 4.43Gpr19 3.34 3.53 3.38Asah3l 2.41 4.05 3.22Mrps10 2.71 3.51 3.22Gins4 2.94 3.14 3.34Sdhc 2.94 4.31 2.05Extl1 2.82 2.93 2.97Coq5 2.29 2.98 3.44Gfer 2.63 3.24 2.79C4b 3.1 3.44 2.11Habp4 2.4 2.59 3.45Lbh 2.1 3.17 3.01Hmgcl 2.38 3.38 2.28Phactr4 2.49 3.46 1.91Ebna1bp2 2.06 2.41 2.93Nol4 2.28 2.39 2.63Pfdn2 1.71 2.56 2.93

Pex6 2.6 2.5 2.03Gng10 2.4 2.09 2.63Eif2ak2 1.74 2.68 2.67Aurkaip1 1.62 3.59 1.66Sepn1 2.05 2.04 2.48Creb3 2.44 1.93 2.02Rnaseh2c 2.09 1.75 2.5Fbxo6 2.03 2.63 1.67D4Ertd22e 2.1 1.99 1.93Tctex1d2 1.51 2.02 2.24Sumf2 2.25 1.8 1.6Tsr2 1.77 2.11 1.53Rasgrf1 1.57 1.68 2.07Ddx51 1.66 1.52 1.83

B3galt6 -11.72 -14.45 -15.07A330021E22Rik -9.25 -11.79 -12.71Ccng2 -10.09 -10.59 -11.1Clstn1 -9.55 -10.22 -9.86Taf6 -7.92 -10.87 -10.15Uap1 -9.6 -9.4 -8.62Zfp365 -8.66 -9.72 -8.04LOC433801 -8.02 -5.47 -11.52Zfp68 -7.33 -8.57 -9.01Rps15a -5.82 -7.65 -10.02Ccl27 -5.58 -8.43 -5.93Lrrc57 -6.21 -6.1 -5.49Bsdc1 -5.06 -6.39 -5.7LOC545056 -4.74 -5.66 -5.63Mod1 -4.59 -5.53 -5.092810410P22Rik -5.57 -5.05 -4.44Klhl21 -4.83 -5.17 -4.97Arhgef19 -4.25 -5.06 -4.67Arl5a -4.93 -5.24 -3.78Atp2c1 -3.9 -4.69 -5.17Atpbd1b -4.14 -5.27 -3.92BC017643 -3.78 -4.69 -4.77Aldh1a1 -2.91 -4.28 -5.49Lars2 -3.6 -4.76 -4.17Fgfr1op2 -2 -5.26 -5H2-T23 -4.06 -3.14 -4.79Dhrsx -2.81 -3.81 -5.2Lgals3bp -2.86 -5.5 -3.321200015F23Rik -3.21 -3.3 -5.11Rab3b -1.93 -5.89 -3.8Vangl2 -2.93 -3.8 -4.79

Rshl2a -2.58 -3.79 -5.01Sbf1 -3.63 -4.43 -3.31LOC100046039 -2.46 -4.66 -3.51Pik3r4 -2.02 -3.74 -4.64Tfrc -4.23 -2.99 -3.15Epm2aip1 -4.89 -2.03 -3.07Yipf4 -3.3 -3.52 -3.12Slc35a5 -2.74 -3.89 -3.15Anxa3 -1.8 -2.73 -5.26330503K22Rik -1.75 -1.65 -6.25Mal -5.1 -2.3 -2.16Ddr1 -2.18 -3.81 -3.55Mog -4.27 -2.73 -2.4AI316807 -2.78 -3.41 -2.94Mrps7 -3.54 -2.06 -3.42Tspan2 -3.39 -2.2 -3.36Trem2 -2.9 -2.84 -2.98Fbxo44 -2.5 -3.23 -2.93Dusp7 -2.68 -3.25 -2.53Pdlim2 -3.02 -2.55 -2.81Rnf41 -3.02 -3.06 -2.29Caskin1 -2.66 -2.87 -2.79Arl6ip1 -2.35 -3.42 -2.46Tsc2 -2.96 -1.7 -3.55Ppox -2.18 -3.56 -2.45Ccndbp1 -2.31 -2.51 -3.29Pex19 -2.32 -2.53 -3.22Abcd3 -2.33 -2.83 -2.88Them4 -2.37 -3.3 -2.32Extl2 -3.33 -2.66 -1.82Actl6b -3.27 -2.85 -1.68Rabl2a -2.58 -2.24 -2.95Bckdhb -3.01 -1.6 -3.08Ank2 -2.74 -2.56 -2.07Gpr17 -2.81 -1.93 -2.5Med23 -2.26 -2.44 -2.43Ttyh2 -2.59 -2.72 -1.71Ivd -2.36 -2.49 -2.12Ifit3 -1.91 -3.01 -2.04Med8 -2.45 -1.78 -2.31Spsb3 -2.29 -2.4 -1.64Enpp4 -2.31 -1.83 -2.08Ttc17 -1.88 -2.45 -1.84Mobp -2 -1.78 -2.39Ociad1 -1.72 -1.96 -2.35Copa -2 -1.64 -2.12

Hexdc -1.92 -2.16 -1.64Dctd -1.76 -2.05 -1.7

Mns1 4.94 4.41Vps33a 3.89 2.74Cpne9 3.66 1.66Snx30 3.48 3.76B4galt3 2.85 2.36Nptx2 2.75 1.56Nudc 2.67 1.722610524H06Rik 2.6 1.87A930025D01Rik 2.28 2.04Pvalb 2.09 1.95Rftn1 2.05 1.72Poldip3 1.91 1.5Zfp612 1.87 1.59Ppt1 1.85 3.2Gmpr 1.81 1.78Atp13a4 1.77 2.03Lsm2 1.6 1.63Dtna 1.56 3.09Mcph1 1.56 1.76Prkab1 1.53 1.5Tnfrsf21 1.53 2.66Copg 1.52 1.51Vgf 1.51 2.3

Camk2d 4.59 1.971110017D15Rik 4.24 2.19Hn1 3.06 1.514922503N01Rik 2.84 1.57Acy1 2.25 1.71H1fx 2.24 1.64Pacrg 2.12 1.95Frmd3 1.94 4.35Rwdd3 1.91 2.15Unc45a 1.9 1.99LOC100048083 1.82 1.6Chchd8 1.79 2.89Chchd5 1.72 1.68LOC100044159 1.7 2.6Abcg1 1.63 1.85Zxdc 1.63 2.22Stmn1 1.63 1.58Eno1 1.57 2.98

Rps6ka1 1.55 1.72

BC032265 10.89 13.56Stbd1 7.47 10.35Trim2 5.2 6.26Col9a2 5.12 2.11Sfrp1 4.81 6.02Epha3 4.16 2.07Tmem91 4 3.34Pgam2 3.72 2.78Ntng1 3.63 2.02Ttl 3.52 3.11BC048546 3.5 4.02Nrsn2 3.48 1.59Moxd1 3.41 2.14Wnt7b 3.11 1.77Ddo 2.96 2.09Sap130 2.83 1.93Top1mt 2.81 2.48Chga 2.76 1.81Cdkal1 2.67 4.96Col4a2 2.57 1.8Lrrc33 2.39 1.79Rgs12 2.38 1.87LOC677317 2.35 3.22Dapk1 2.21 3.22Tspo 2.2 3.09Wnt2 2.2 2.98Polb 2.08 2.04BC056474 2.04 1.59Rnps1 2.01 1.9Mmab 2.01 1.71700037H04Rik 1.99 1.65Muted 1.96 1.58Epha7 1.96 1.68Cadm3 1.96 3.07Unc5c 1.89 2.29Gpr162 1.82 1.53Tdrd7 1.81 1.55Grip1 1.8 1.72LOC100048046 1.76 1.93Ahdc1 1.73 2.851810009N02Rik 1.71 1.82Nhlrc1 1.66 1.77Mybbp1a 1.65 1.7Zfp692 1.56 2.24

Sec14l1 1.56 1.98Banf1 1.55 1.67Nme3 1.53 1.88Haghl 1.5 2.48

Clcn7 -1.63 -2.1Gnptg -1.76 -2.68Nit1 -1.78 -2.04Dusp26 -1.84 -1.51Hspa2 -1.95 -1.57Zeb2 -2.2 -3.57Cpsf3l -2.26 -2.43Gbp2 -2.4 -2.9Id4 -2.43 -2.24Ehd3 -2.57 -2Ccrn4l -2.65 -2.11Scarf2 -2.76 -1.62Traf7 -2.82 -1.51Usp21 -2.86 -1.58Tmod1 -3.72 -2.25Ush2a -3.82 -3.24C030030A07Rik -4.74 -5.73Myoc -7.68 -7.87

Plekhg3 -1.57 -2.08Bzw1 -1.59 -1.81Lass2 -1.62 -1.731700084C01Rik -1.65 -2.152310016C16Rik -1.68 -2.25Evi2a -1.73 -1.54Slc12a2 -1.77 -1.74Fa2h -1.78 -1.83Rgs7 -1.78 -1.85Pebp1 -1.91 -3.71Hist1h1c -1.96 -2.66Ufc1 -2.02 -1.82Pla2g7 -2.02 -3.61Grb14 -2.06 -2.52Iigp2 -2.09 -2.46Mag -2.14 -1.82B2m -2.18 -2.55Emp2 -2.23 -1.57Darc -2.26 -2.58Acaa2 -2.35 -1.99Bcas1 -2.37 -2.05Cbwd1 -2.6 -2.91

Lims2 -2.63 -1.84Nudt4 -2.9 -1.56Cldn11 -2.97 -3.88LOC100044190 -3.08 -3.18Arid5b -3.44 -1.51Hbb-b1 -3.67 -6.38Iap -4.46 -4.37

Itm2a -1.57 -2.1Faah -1.61 -1.53Trak2 -1.63 -2.43Ncald -1.64 -2.11Lmna -1.66 -1.98Actb -1.69 -2.494933421E11Rik -1.71 -1.52Kbtbd2 -1.71 -1.59Ppp3ca -1.74 -1.94Mfn2 -1.76 -2.22Hebp1 -1.8 -3.1Ppm1m -1.86 -2.03Eif2ak1 -1.93 -1.6Pou6f1 -2 -1.59Exoc2 -2.01 -1.73Krt222 -2.02 -1.92Zbtb17 -2.02 -1.57Cnp -2.08 -1.61Cyp51 -2.09 -2.28Rab6 -2.14 -1.54Atf4 -2.2 -2.428430419L09Rik -2.22 -2.11Pdk1 -2.23 -2.54Socs6 -2.43 -2.84Bok -2.45 -2.47Hr -2.68 -1.81Itpka -2.78 -2.28Rasgrp1 -2.81 -4.1LOC100041290 -2.84 -3.07Zfp160 -2.87 -2.27Zfp101 -2.96 -3.25Kif5a -3.04 -1.76LOC100043257 -3.24 -2.8Pigz -3.34 -1.69Sytl2 -3.39 -2.7Ddx6 -3.43 -1.6Cd200 -3.45 -2.352310010B21Rik -3.61 -4.22

Cort -3.64 -4.68Zfp691 -3.64 -2.34Tmc7 -4.06 -3.63Kif21a -4.15 -2.15Zfp367 -4.43 -3.19Axud1 -4.49 -2.51Mef2c -4.79 -2.43Tagap -4.87 -5.29Rpl29 -4.87 -6.3Agxt2l1 -5.01 -4.93Slc25a18 -5.21 -5.26Atp8a1 -6.48 -6.27Agpat5 -6.5 -7.17Kif1b -7.34 -2.12Ccl21b -13.85 -11.55

Hpcal1 6.64Smpdl3b 5.71Trhr 5.2EG245297 4.61Marcksl1 4.3Wfs1 4.19Ly6g6e 4.02Dcn 3.97Lpin2 3.87Apaf1 3.76Inpp4b 3.58Sez6 3.43Tnnt1 3.41Fibcd1 3.23Tbc1d8 3.09Cacna1h 2.99Odz3 2.99Gpr83 2.98Pnck 2.96Cmbl 2.86LOC100046744 2.84Cpne6 2.8Fahd2a 2.76Grb7 2.74Cachd1 2.71Cdh13 2.64Syt9 2.63Mcm6 2.62Cpne2 2.62Mrps15 2.57

Crtac1 2.57Mettl1 2.54Dlx2 2.53Tmem35 2.51Pdyn 2.48Tnfaip8 2.47Slc29a1 2.464932409I22Rik 2.46Tiam1 2.44Dpysl3 2.41Gnai2 2.41Emid2 2.4Usp11 2.39Kirrel3 2.38Ssbp3 2.37Gfap 2.36Lamc1 2.34Lin7b 2.34Reln 2.23Rsph1 2.23Slc25a1 2.22Lrrc1 2.22Rgs17 2.22Palm 2.22Syngr3 2.21

3/8/2012 0:00 2.21Dgkg 2.2Tmem150 2.19Igsf9 2.18Tmem176b 2.18Taf10 2.182310003L22Rik 2.17Mlp 2.16Lgals1 2.15Ntsr1 2.15Ass1 2.14Hexim2 2.13Gas2l1 2.13Casc4 2.13Lrrfip1 2.09Bzw2 2.09Tmsb10 2.07Klhl2 2.06Acvr2a 2.06Cpsf1 2.05Csf2ra 2.05

Tsen2 2.05Srp14 2.04Bloc1s1 2.02Mrpl54 2.02Mapk12 2.01Sox12 2.01Cdc42se2 2Chl1 21500010J02Rik 1.99Mctp1 1.99Plch2 1.98Slc9a6 1.98Acot8 1.96Igsf11 1.96St6galnac5 1.94Igfbp2 1.94Gm1673 1.93Fkbp5 1.92Smarcc1 1.92BC004004 1.91A030009H04Rik 1.9Trim9 1.9Setd6 1.89Ercc1 1.89BC068157 1.87Mapbpip 1.87Aloxe3 1.87Asb3 1.87BC021381 1.86Th1l 1.86Alcam 1.85U2af2 1.84Zcchc3 1.84Emr1 1.83Acsf3 1.83Nup88 1.82Med11 1.81Trps1 1.8Ifngr1 1.82410022L05Rik 1.8Cog1 1.79E4f1 1.79Elmo1 1.78Rapgef5 1.782510006D16Rik 1.78

Ldoc1l 1.77Drctnnb1a 1.771110039B18Rik 1.76Psmc3ip 1.75Mbd4 1.75Zfp385a 1.74B230373P09Rik 1.741700030K09Rik 1.74Ncdn 1.74Samd10 1.73Apitd1 1.73Lrpap1 1.73Zbtb39 1.73Ccdc23 1.72Pum1 1.721200011O22Rik 1.72Xrcc6 1.72Lgtn 1.72Zfp318 1.71Hook2 1.71Scamp3 1.71Lrrc16b 1.7Cntn6 1.7Pfn1 1.69Atp2b4 1.69Dmrtc1a 1.68Mtap7d1 1.68Dlx1 1.68Kcnip3 1.67Dhcr7 1.67Hcn3 1.66Mccc1 1.66Fgfrl1 1.65Prkrip1 1.65Ssh3 1.65Calca 1.64Lst1 1.64Smyd3 1.64Efs 1.63Pom121 1.63Prdx5 1.63Trpt1 1.62Sla 1.62Gsk3a 1.62Caln1 1.621200013P24Rik 1.62

Pak3 1.62Nsmce1 1.61Tha1 1.616720458F09Rik 1.61Kif21b 1.6St7 1.6Bola2 1.59Spire2 1.59Ifitm2 1.59Slc39a6 1.59Gtpbp6 1.59Map3k3 1.59Tmem117 1.59Hist1h2bk 1.58Ovgp1 1.58Rai14 1.58Galntl4 1.58Mtvr2 1.56Igsf21 1.56Efemp2 1.54Psme1 1.54Fkbp2 1.54Slc5a6 1.54Mtx1 1.54Tgs1 1.54Spsb4 1.53Gspt2 1.53Lca5 1.52Actr8 1.52Trappc4 1.52Syf2 1.52Fpgs 1.52Tmem107 1.511700025G04Rik 1.51Pacsin1 1.51Scarb2 1.5

Gabrd -1.5Ccdc127 -1.5Mdga2 -1.51Stard8 -1.51Inppl1 -1.52Acsl6 -1.52Igfbp6 -1.52Klhdc3 -1.53Rgs6 -1.53

Yaf2 -1.54Dmrta2 -1.55LOC100047651 -1.552300002D11Rik -1.55Mrpl9 -1.56Ndufaf1 -1.56Prei4 -1.57Epdr1 -1.58Dguok -1.59Irf9 -1.59Ppm1b -1.59Srd5a3 -1.6Idh3g -1.6Cyb5r4 -1.61Nars -1.61Gpr34 -1.62Arhgap15 -1.62Stt3b -1.63Necab3 -1.63Fbxo7 -1.63Napa -1.63Herpud1 -1.64Wdr8 -1.64Rtn4 -1.65Ugt8a -1.66Snrpd1 -1.67Camkk1 -1.67Lrrc6 -1.67AW549877 -1.68Spire1 -1.68Pcgf5 -1.68Golm1 -1.69Mtap7d2 -1.69Asf1a -1.69Fbxo27 -1.7Cacng2 -1.71Bcor -1.72Kcnb1 -1.73Fbxo32 -1.73Gria3 -1.74Iars2 -1.74LOC382010 -1.76Rpl7l1 -1.77Mgll -1.78Msc -1.78Dpm1 -1.8

Psat1 -1.8Mapk10 -1.8Jdp2 -1.81Cadm2 -1.81Mrpl48 -1.84Aldh1l1 -1.85Ap1s1 -1.86Wdr22 -1.86Scd2 -1.86Fez2 -1.87Pde1a -1.87Kcnk4 -1.88Stmn4 -1.88Cadps2 -1.88Ppm2c -1.88Enpp6 -1.89Fndc5 -1.9Arid4b -1.91Gabrg2 -1.91Stk17b -1.93Mkks -1.93Cdc2l1 -1.94Parva -1.94LOC100046035 -1.96Wipi1 -1.96Msi2h -1.97Kcnu1 -1.97Sbds -1.98C130038G02Rik -1.98Frmd6 -1.99Rab5a -1.99Tceb1 -2.01Dfna5h -2.01Strbp -2.02Ephb6 -2.02Ddef2 -2.05Ola1 -2.08Dbc1 -2.092610034M16Rik -2.11Tmem49 -2.12E2f1 -2.14Tek -2.15Slc40a1 -2.16Crebl2 -2.22Lsm12 -2.22Ccdc132 -2.23

Ep300 -2.24Hsd17b7 -2.27Snx13 -2.28LOC100046781 -2.29Rora -2.31Ncam2 -2.32Igtp -2.32Lgi3 -2.33Cacnb4 -2.35Frag1 -2.37Ing3 -2.37Cited4 -2.38Iqgap1 -2.38C1qdc2 -2.39D1Bwg0212e -2.39Ykt6 -2.41Tssc1 -2.41Mat2b -2.42Slc45a1 -2.45Ufsp2 -2.46Centg1 -2.48Nos1ap -2.49Rsrc2 -2.49Zfp326 -2.5Ubxd4 -2.5Syt1 -2.52Akap7 -2.55Ifngr2 -2.56Rcan2 -2.59Pgm2l1 -2.61Camkk2 -2.65Rpap1 -2.661700040I03Rik -2.68Atf7ip -2.68Eps15 -2.68Gpr135 -2.69Cbx5 -2.74Kcnip4 -2.75Myl4 -2.78Ociad2 -2.8Pak7 -2.84Grin1 -2.88Klhl9 -2.91Ramp3 -2.96Rapgef4 -2.97Lrg1 -3

Rock2 -3.02Camk1d -3.05Ctsk -3.07Hnrph2 -3.16Zfp91 -3.17AI593442 -3.172310010M24Rik -3.21Csnrp3 -3.36Morf4l1 -3.44Pik3ca -3.47Zfp238 -3.5Ccdc85a -3.56Tmprss7 -3.57Llgl2 -3.6Tmem10 -3.63Rorb -3.64Dkk3 -3.76Tmem132d -3.84Zmynd11 -3.93Rnmt -3.98Cnksr2 -4Zfp91-cntf -4.05Ash1l -4.07Zfp758 -4.21Plcb4 -4.21Cit -4.24Ubtd2 -4.33D17Wsu92e -4.34Creg2 -4.36Ankrd12 -4.38Bach1 -4.4Flrt2 -4.431110057K04Rik -4.44Zfp106 -4.61Spag9 -4.79Tex9 -4.88Osbpl6 -5.15Lsamp -5.43Abcd2 -5.44R3hdm1 -5.66Btbd3 -5.95Slc7a14 -6.54Scn1a -6.61Nsd1 -7.3Zzz3 -7.38Kitl -8.16

Megf9 -8.18Zbtb7a -8.29Atp2b2 -8.35Nell2 -9.55Kcna1 -9.57Ccl21c -12.31

Atp10d 7.88Cd74 4.43Igsf1 4.06Wbp11 4.03Nts 3.87Rbm43 3.82Osbpl3 3.78Ogn 3.616330406I15Rik 3.51D8Ertd82e 3.26Rerg 3.15Uchl5 3.13Nefm 3.1Sncg 2.83Pea15 2.77LOC100045304 2.7Smc5l1 2.67Slc17a5 2.64Col16a1 2.59Itgb4 2.57Emp1 2.52Tmsb4x 2.52Cdh22 2.5Olfml2b 2.472210016L21Rik 2.44Fip1l1 2.43Rreb1 2.41810074P20Rik 2.31Arl6ip4 2.3Cidea 2.3Nola3 2.29Bcl6 2.27Gad1 2.27N6amt1 2.25Tpd52l1 2.24Ndufb5 2.24Pkp2 2.22Tmem74 2.21LOC100046996 2.21

Scyl1 2.19Pafah1b3 2.171500032D16Rik 2.17Hectd2 2.16LOC100045780 2.16Ghitm 2.15Sc5d 2.14Rangrf 2.13Gmpr2 2.12Slc2a1 2.1Tesk1 2.093632451O06Rik 2.06Magee1 2.02Raver2 2.01Per2 2.01Brdt 2Golga3 1.99Inhba 1.99Srxn1 1.98Abca3 1.97Cib2 1.96Ufsp1 1.95Vti1b 1.95Pmp22 1.94BC002163 1.941810043G02Rik 1.93Chrnb2 1.92Zmpste24 1.910610007P22Rik 1.91Ntn2l 1.89Stard3 1.89Mpp6 1.89Cdkl2 1.88Lefty1 1.86Trappc1 1.85Brsk2 1.84Smarcd3 1.84Abat 1.83Lgals8 1.832310016E02Rik 1.82Nicn1 1.8Fkbp1b 1.8Glrx2 1.8Zcchc12 1.78Aven 1.78Sdcbp 1.78

Hus1 1.77Psmb10 1.76B020018G12Rik 1.76Pftk1 1.76Mrps21 1.75Rhbdl7 1.75Xpa 1.75Tceb2 1.73Tmem85 1.73Prdm8 1.73Rdm1 1.71Dtnb 1.7Fabp3 1.7Frs3 1.7Slc35b1 1.69C85492 1.68Mtpn 1.67Cnot7 1.67Arpc5 1.67Psip1 1.66Cdc2l5 1.66Pih1d1 1.66LOC100039571 1.65Tnfrsf25 1.65Polr2j 1.65Prps1 1.65B230342M21Rik 1.64Pycrl 1.64Psma4 1.63Mrpl52 1.63Prkcd 1.62Vkorc1l1 1.61Elovl5 1.61Txnl4a 1.61Caly 1.61Sri 1.61Arl3 1.61Stk32c 1.6Appbp1 1.66330403K07Rik 1.59Palmd 1.59Grik1 1.59Gstp1 1.59Commd1 1.59Sec24d 1.59

2810428I15Rik 1.59Mrpl13 1.58Lpl 1.58Cdo1 1.58LOC100044862 1.58Plod3 1.57Prnpip1 1.57Abhd3 1.57Tgm2 1.56C1qbp 1.56Rps27l 1.56Anapc2 1.56Faim 1.55Chi3l1 1.55Nagk 1.55Lrrn1 1.55LOC676420 1.54Cbr1 1.54Tspan17 1.53Cugbp2 1.53Elavl2 1.52Med21 1.52Ndufa8 1.52Robo1 1.51

Pknox2 -1.51Gde1 -1.51Pex11b -1.51Phldb1 -1.51Kcnc4 -1.51Pip5k1a -1.51Cux2 -1.52Rassf1 -1.52Phka1 -1.53BC046404 -1.53Tprkb -1.53Coq7 -1.53Trafd1 -1.53Wdr70 -1.55Wiz -1.56LOC100045343 -1.56Map2k3 -1.56Eprs -1.582410025L10Rik -1.58Ypel1 -1.58Prkcz -1.59

Stard7 -1.59Bzrap1 -1.6Nphp1 -1.6Adcy8 -1.61Sesn1 -1.61Cant1 -1.63Prpf39 -1.64Tnip1 -1.65Ift122 -1.66Pfkp -1.67Med10 -1.67Fmo1 -1.67Trrap -1.67Tom1 -1.67Atp6ap2 -1.67Mboat2 -1.69F730014I05Rik -1.69Zadh1 -1.69Paip2b -1.69Rab7 -1.69Nrg3 -1.7Mfap1b -1.71Rpo1-3 -1.71Anapc5 -1.72Igsf3 -1.72Cyp2j9 -1.72Ift81 -1.73Slc35e1 -1.73Aste1 -1.73Rbm18 -1.73Dact2 -1.75Hprt1 -1.75Tsc22d4 -1.75LOC100045887 -1.76Crkl -1.76Khdrbs1 -1.762700081O15Rik -1.77Ube2o -1.78Cecr6 -1.79Cdkn2c -1.79Rims3 -1.79Zfp87 -1.79Otx1 -1.796430571L13Rik -1.8Dcx -1.83Obfc2b -1.84

Nsmaf -1.84Hist1h2ad -1.85Ivns1abp -1.87Bmpr1a -1.87Gpc5 -1.88Tardbp -1.89Sox21 -1.91Tshz3 -1.92Fn3k -1.92Cacng3 -1.93Pcyox1l -1.95Islr2 -1.98Rnf6 -1.99Apcdd1 -1.99Decr2 -2.01Centb5 -2.02Fads2 -2.04Sema3a -2.06Klc4 -2.09Prkaa2 -2.1Ccno -2.11LOC100047619 -2.11Atp1a2 -2.12Chac1 -2.12Sorbs1 -2.12Prune -2.12Smox -2.16Dusp18 -2.16Galnt1 -2.17Acpl2 -2.18Slc8a2 -2.2Mrp63 -2.2Wnt10a -2.21AI413582 -2.27Pik3ip1 -2.28Zfp213 -2.29Hagh -2.29Cox6a2 -2.31Acot7 -2.32Tmem8 -2.34Npr2 -2.36Gldc -2.39Ddit4l -2.39Lrrc20 -2.39Cxcl12 -2.44Itfg2 -2.44

LOC100047934 -2.45Phlda3 -2.47Ndufs2 -2.51Bex4 -2.51LOC100045439 -2.52Psmd9 -2.55Ccnd1 -2.57Fbxl18 -2.61Scnm1 -2.62Rgl1 -2.67Sh3gl2 -2.74E430002G05Rik -2.751700027J05Rik -2.78Sv2a -2.78C230078M08Rik -2.79Hrbl -2.82Mto1 -2.87Cckbr -3.06Bach2 -3.08Rnd3 -3.1Syt17 -3.21Rasl10a -3.41Chd5 -3.67Msr2 -3.7Abcc10 -3.792610020C11Rik -3.95Arsj -4.27Spink8 -4.75Mgst1 -10.42Fcer1g -22.31

H2-Bl 16.47Npvf 7.52Pgk1 6.24Vps39 4.38Apoc1 4.06Rec8 3.58Lmo3 3.55Dnajb4 3.34Glipr2 3.23Ankrd56 3.21Vip 3.15Lck 3.04Nmu 2.67Sorl1 2.64

Fgf1 2.64Pi16 2.56BC055368 2.5Clic6 2.48Crhbp 2.432410004L22Rik 2.42Gstm6 2.39Ngb 2.39Kptn 2.37Gm129 2.324930539E08Rik 2.31Arhgap27 2.28Pvrl3 2.28Gnas 2.26Ccdc28a 2.25Amn 2.24Ganc 2.25133401N09Rik 2.2Poll 2.2Mycbpap 2.18Immp2l 2.18Rpl22 2.16Vti1a 2.13Pcsk2 2.12Clip1 2.07Slc5a5 2.06Ddit3 2.04As3mt 2.03Ctnnd2 1.96Cenpl 1.96Ctps 1.96Dclk3 1.95Pdia4 1.94Faim2 1.93Irak2 1.93Ap4m1 1.91Shf 1.9Slc6a15 1.9Dhps 1.89Synj2bp 1.88BC057371 1.86Preb 1.85Tmem62 1.85Lphn3 1.84Rabggta 1.83Tarsl2 1.83

Rtn2 1.8Ethe1 1.79Donson 1.78Eif3s1 1.78Freq 1.77Il17d 1.76Peg10 1.742610042L04Rik 1.74Tjap1 1.74Nrip3 1.74Ttll1 1.73Dbpht2 1.73Gfm1 1.72Srprb 1.72Capza1 1.72Pnpo 1.71Gpr89 1.7Mapk3 1.69Ubxd1 1.68Krtcap3 1.66Adam22 1.65Slc7a3 1.65Mrpl17 1.64Tprgl 1.63Tex264 1.62Ars2 1.61Gpx3 1.6Lgals3 1.6Jazf1 1.6Hbegf 1.59Dync1i1 1.59Hnrpc 1.59Car11 1.58Tmem143 1.58Peli3 1.58Trim11 1.57Garnl4 1.57Eid2 1.56Tbc1d5 1.56Mad2l2 1.56Zfp398 1.562610524A10Rik 1.55Bai3 1.54Cog6 1.532610024G14Rik 1.53Zfand2b 1.53

Tmem186 1.52Dvl1 1.52Sppl3 1.52B230339H12Rik 1.51Srm 1.51Cds2 1.51Zfp704 1.51

4933434I06Rik -1.52Hs3st1 -1.53Echdc2 -1.53Rell1 -1.55Them2 -1.55Usp10 -1.56Prei3 -1.561810035L17Rik -1.59Dnaja2 -1.62Timp3 -1.62Peci -1.65Hist1h2af -1.66Sypl -1.66Tcf4 -1.67Dpysl4 -1.69Mapk4 -1.69Fastkd5 -1.7Myh9 -1.72Litaf -1.73Cml4 -1.73Arpc1b -1.74Pcca -1.75Lamp2 -1.75Armc9 -1.75Sirt2 -1.76Rras2 -1.76Itm2b -1.77Swap70 -1.772610002J02Rik -1.77Hfe -1.77Gja1 -1.79Unc5b -1.8Mrpl50 -1.8Lyrm5 -1.82LOC100044779 -1.85Wscd1 -1.87Pldn -1.88S100b -1.9

Slc4a2 -1.92Purb -1.94Nhsl1 -1.96Cct5 -1.98Rgma -1.98Slc16a6 -1.99Rrp1 -1.99Riok1 -2.02Gosr2 -2.03Car14 -2.04Il11ra1 -2.05Clic4 -2.05Necap2 -2.062900064A13Rik -2.16Cryzl1 -2.16Dci -2.26Cd59a -2.28Zfp36l1 -2.29S100a10 -2.35H2-M3 -2.41Rab3c -2.42Dab2 -2.45Pllp -2.53Gpr177 -2.55Ptprb -2.59E130012A19Rik -2.69Rbmx -2.71Rps3a -2.761700029J07Rik -2.78Pgm2 -2.78Slc7a11 -2.79Agtrap -2.88Sparcl1 -2.89Anxa2 -2.97Bid -2.99Pkd2l1 -3.02Elavl4 -3.03LOC100047749 -3.03Hist2h2be -3.142210012G02Rik -3.34Edg2 -3.42S100a11 -3.442810432L12Rik -3.6Ptp4a2 -3.63Samd9l -3.69Ifi27 -3.81

Slc38a2 -3.84Bgn -3.9Ppic -3.99Arhgap29 -4Xkr8 -4.04Olfml3 -4.28Srgn -4.76Lyzs -5.04Lyz -6.26Gjb2 -6.42Oasl2 -6.42Thbd -7.23Nupr1 -7.83Gramd4 -18.99

Tmem132e 3.91 -1.59Rin3 1.59 -1.65Pcmt1 -2.07 1.97Zfp828 -2.25 1.91Socs2 -2.63 2.03

Rims1 -2.1 1.95

Usf1 3.38 -3.43Trib2 2.83 -1.63Cox6c 1.5 -1.71Fgf12 -1.57 1.75Phactr1 -1.63 1.95Mrpl3 -1.98 1.71

Ccrk 2.23 -1.75 1.93Lix1 -2.03 -2.02 2.2

Table S5. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO cortex compared to WT controls. Significantly-populated canonical signaling pathways, generated using the transcripts significantly and differentially regulated in T1R3KO cortex compared to WT controls, are depicted. The pathway enrichment probability (stated as –log 10(p-value)) and enrichment ratio are stated. The predicted z-score (where available), indicating a preference for ‘activation’ (positive scores) or ‘inhibition’ (negative scores) of the specific pathways are stated. The percentages of the total pathway genelist (indicated as number of experimental transcripts identified/total transcripts linked to the pathway) populated by down- (Downregulated) or upregulated (Upregulated) transcripts from the input datasets are also indicated. A percentage pathway population, by the experimentally identified transcripts is indicated in parentheses.

Ingenuity Canonical Pathways -log(p-value) Ratio z-score Downregulated Upregulated Calcium Signaling 5.70E+00 1.01E-01 -1.732 13/178 (7%) 5/178 (3%)Role of NFAT in Cardiac Hypertrophy 4.46E+00 8.94E-02 -0.775 9/179 (5%) 7/179 (4%)CREB Signaling in Neurons 4.13E+00 8.77E-02 -0.277 7/171 (4%) 8/171 (5%)CXCR4 Signaling 4.12E+00 9.21E-02 -0.577 7/152 (5%) 7/152 (5%)Signaling by Rho Family GTPases 4.08E+00 7.69E-02 -1.807 10/234 (4%) 8/234 (3%)Cardiac Hypertrophy Signaling 3.84E+00 7.62E-02 -1 9/223 (4%) 8/223 (4%)NGF Signaling 3.77E+00 1.03E-01 0 6/107 (6%) 5/107 (5%)GNRH Signaling 3.65E+00 9.30E-02 0.577 5/129 (4%) 7/129 (5%)Ephrin Receptor Signaling 3.50E+00 8.05E-02 6/174 (3%) 8/174 (5%)B Cell Receptor Signaling 3.45E+00 7.95E-02 7/176 (4%) 7/176 (4%)14-3-3-mediated Signaling 3.43E+00 9.40E-02 5/117 (4%) 6/117 (5%)CCR3 Signaling in Eosinophils 3.43E+00 9.40E-02 5/117 (4%) 6/117 (5%)P2Y Purigenic Receptor Signaling Pathway 3.37E+00 9.24E-02 -0.632 5/119 (4%) 6/119 (5%)Synaptic Long Term Potentiation 3.37E+00 9.24E-02 6/119 (5%) 5/119 (4%)Angiopoietin Signaling 3.37E+00 1.21E-01 0.447 5/66 (8%) 3/66 (5%)G-Protein Coupled Receptor Signaling 3.14E+00 6.64E-02 11/256 (4%) 6/256 (2%)PAK Signaling 3.14E+00 1.01E-01 -0.333 5/89 (6%) 4/89 (4%)Axonal Guidance Signaling 3.04E+00 5.54E-02 10/433 (2%) 14/433 (3%)FLT3 Signaling in Hematopoietic Progenitor Cells 3.04E+00 1.08E-01 4/74 (5%) 4/74 (5%)cAMP-mediated signaling 2.97E+00 6.85E-02 -0.832 10/219 (5%) 5/219 (2%)Dendritic Cell Maturation 2.88E+00 7.26E-02 9/179 (5%) 4/179 (2%)Leucine Degradation I 2.82E+00 3.33E-01 1/9 (11%) 2/9 (22%)Calcium Transport I 2.82E+00 3.33E-01 2/9 (22%) 1/9 (11%)ERK5 Signaling 2.79E+00 1.11E-01 0.378 3/63 (5%) 4/63 (6%)Estrogen-Dependent Breast Cancer Signaling 2.79E+00 1.11E-01 -0.816 5/63 (8%) 2/63 (3%)Paxillin Signaling 2.74E+00 8.91E-02 -1 6/101 (6%) 3/101 (3%)Melanocyte Development and Pigmentation Signaling 2.68E+00 9.52E-02 -0.707 5/84 (6%) 3/84 (4%)Interferon Signaling 2.66E+00 1.47E-01 0 3/34 (9%) 2/34 (6%)Neurotrophin/TRK Signaling 2.63E+00 1.04E-01 -0.378 4/67 (6%) 3/67 (4%)Thrombin Signaling 2.62E+00 6.81E-02 -0.302 6/191 (3%) 7/191 (4%)Breast Cancer Regulation by Stathmin1 2.62E+00 6.81E-02 7/191 (4%) 6/191 (3%)Role of NFAT in Regulation of the Immune Response 2.57E+00 7.02E-02 -0.632 6/171 (4%) 6/171 (4%)Molecular Mechanisms of Cancer 2.57E+00 5.48E-02 10/365 (3%) 10/365 (3%)Agrin Interactions at Neuromuscular Junction 2.56E+00 1.01E-01 0 3/69 (4%) 4/69 (6%)UVA-Induced MAPK Signaling 2.55E+00 9.09E-02 0 4/88 (5%) 4/88 (5%)RhoGDI Signaling 2.53E+00 6.94E-02 6/173 (3%) 6/173 (3%)Chemokine Signaling 2.49E+00 9.86E-02 0.378 3/71 (4%) 4/71 (6%)Renal Cell Carcinoma Signaling 2.49E+00 9.86E-02 -0.447 5/71 (7%) 2/71 (3%)STAT3 Pathway 2.42E+00 9.59E-02 4/73 (5%) 3/73 (4%)

AMPK Signaling 2.41E+00 6.70E-02 -1 9/179 (5%) 3/179 (2%)tRNA Charging 2.40E+00 1.28E-01 3/39 (8%) 2/39 (5%)Wnt/Ca+ pathway 2.38E+00 1.07E-01 -0.816 4/56 (7%) 2/56 (4%)Cholesterol Biosynthesis I 2.32E+00 2.31E-01 2/13 (15%) 1/13 (8%)Cholesterol Biosynthesis II (via 24,25-dihydrolanosterol) 2.32E+00 2.31E-01 2/13 (15%) 1/13 (8%)Cholesterol Biosynthesis III (via Desmosterol) 2.32E+00 2.31E-01 2/13 (15%) 1/13 (8%)Myc Mediated Apoptosis Signaling 2.31E+00 1.03E-01 3/58 (5%) 3/58 (5%)Gα12/13 Signaling 2.31E+00 7.69E-02 -1.667 6/117 (5%) 3/117 (3%)FcγRIIB Signaling in B Lymphocytes 2.30E+00 1.22E-01 -0.447 3/41 (7%) 2/41 (5%)ILK Signaling 2.28E+00 6.45E-02 -2.111 9/186 (5%) 3/186 (2%)ATM Signaling 2.27E+00 1.02E-01 0.447 4/59 (7%) 2/59 (3%)Neuropathic Pain Signaling In Dorsal Horn Neurons 2.21E+00 8.00E-02 -1.414 6/100 (6%) 2/100 (2%)GM-CSF Signaling 2.16E+00 9.68E-02 0 3/62 (5%) 3/62 (5%)Prostate Cancer Signaling 2.15E+00 8.54E-02 5/82 (6%) 2/82 (2%)PCP pathway 2.13E+00 9.52E-02 0.816 2/63 (3%) 4/63 (6%)Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis 2.08E+00 5.37E-02 8/298 (3%) 8/298 (3%)FGF Signaling 2.06E+00 8.24E-02 -0.378 4/85 (5%) 3/85 (4%)TR/RXR Activation 2.06E+00 8.24E-02 6/85 (7%) 1/85 (1%)ErbB Signaling 2.04E+00 8.14E-02 4/86 (5%) 3/86 (3%)fMLP Signaling in Neutrophils 2.02E+00 7.41E-02 -0.378 4/108 (4%) 4/108 (4%)Ephrin A Signaling 2.01E+00 1.04E-01 3/48 (6%) 2/48 (4%)Fatty Acid β-oxidation I 2.01E+00 1.29E-01 3/31 (10%) 1/31 (3%)Zymosterol Biosynthesis 1.98E+00 3.33E-01 2/6 (33%) 0/6 (0%)Huntington's Disease Signaling 1.97E+00 5.68E-02 7/229 (3%) 6/229 (3%)Type I Diabetes Mellitus Signaling 1.97E+00 7.27E-02 5/110 (5%) 3/110 (3%)Tec Kinase Signaling 1.93E+00 6.33E-02 -1.134 5/158 (3%) 5/158 (3%)Circadian Rhythm Signaling 1.92E+00 1.21E-01 3/33 (9%) 1/33 (3%)Melatonin Signaling 1.91E+00 8.57E-02 -0.816 3/70 (4%) 3/70 (4%)IL-8 Signaling 1.90E+00 5.98E-02 -0.632 6/184 (3%) 5/184 (3%)Germ Cell-Sertoli Cell Junction Signaling 1.89E+00 6.25E-02 6/160 (4%) 4/160 (3%)Dopamine-DARPP32 Feedback in cAMP Signaling 1.88E+00 6.21E-02 -1.667 7/161 (4%) 3/161 (2%)SAPK/JNK Signaling 1.84E+00 7.45E-02 0.378 3/94 (3%) 4/94 (4%)Ephrin B Signaling 1.83E+00 8.22E-02 2/73 (3%) 4/73 (5%)tRNA Splicing 1.83E+00 1.14E-01 2/35 (6%) 2/35 (6%)Phospholipase C Signaling 1.83E+00 5.44E-02 -0.632 8/239 (3%) 5/239 (2%)Prolactin Signaling 1.83E+00 8.22E-02 0.447 5/73 (7%) 1/73 (1%)Role of IL-17A in Arthritis 1.80E+00 9.26E-02 3/54 (6%) 2/54 (4%)Stearate Biosynthesis I (Animals) 1.79E+00 1.11E-01 3/36 (8%) 1/36 (3%)Granzyme A Signaling 1.79E+00 1.50E-01 2/20 (10%) 1/20 (5%)Actin Cytoskeleton Signaling 1.78E+00 5.53E-02 7/217 (3%) 5/217 (2%)IGF-1 Signaling 1.77E+00 7.22E-02 0.447 5/97 (5%) 2/97 (2%)Amyotrophic Lateral Sclerosis Signaling 1.74E+00 7.14E-02 6/98 (6%) 1/98 (1%)Glioblastoma Multiforme Signaling 1.72E+00 6.16E-02 0.707 5/146 (3%) 4/146 (3%)Protein Kinase A Signaling 1.69E+00 4.66E-02 10/386 (3%) 8/386 (2%)Docosahexaenoic Acid (DHA) Signaling 1.67E+00 1.03E-01 2/39 (5%) 2/39 (5%)HIF1α Signaling 1.66E+00 6.86E-02 5/102 (5%) 2/102 (2%)Heme Biosynthesis II 1.63E+00 2.22E-01 1/9 (11%) 1/9 (11%)Rac Signaling 1.62E+00 6.73E-02 -0.378 4/104 (4%) 3/104 (3%)Hereditary Breast Cancer Signaling 1.59E+00 6.20E-02 6/129 (5%) 2/129 (2%)Melanoma Signaling 1.56E+00 9.52E-02 3/42 (7%) 1/42 (2%)Antiproliferative Role of Somatostatin Receptor 2 1.54E+00 7.94E-02 0 2/63 (3%) 3/63 (5%)Renin-Angiotensin Signaling 1.52E+00 6.42E-02 -0.378 4/109 (4%) 3/109 (3%)Pyridoxal 5'-phosphate Salvage Pathway 1.51E+00 7.81E-02 1/64 (2%) 4/64 (6%)Role of IL-17F in Allergic Inflammatory Airway 1.50E+00 9.09E-02 2/44 (5%) 2/44 (5%)

DiseasesNatural Killer Cell Signaling 1.50E+00 6.36E-02 4/110 (4%) 3/110 (3%)FAK Signaling 1.49E+00 6.90E-02 4/87 (5%) 2/87 (2%)ERK/MAPK Signaling 1.48E+00 5.35E-02 -0.632 6/187 (3%) 4/187 (2%)RANK Signaling in Osteoclasts 1.47E+00 6.82E-02 4/88 (5%) 2/88 (2%)Virus Entry via Endocytic Pathways 1.45E+00 6.74E-02 5/89 (6%) 1/89 (1%)GDNF Family Ligand-Receptor Interactions 1.42E+00 7.35E-02 -0.447 3/68 (4%) 2/68 (3%)Role of Osteoblasts, Osteoclasts and Chondrocytes in Rheumatoid Arthritis 1.41E+00 5.02E-02 6/219 (3%) 5/219 (2%)Glucocorticoid Receptor Signaling 1.40E+00 4.73E-02 8/275 (3%) 5/275 (2%)Superpathway of Cholesterol Biosynthesis 1.40E+00 1.07E-01 2/28 (7%) 1/28 (4%)CCR5 Signaling in Macrophages 1.39E+00 7.25E-02 1/69 (1%) 4/69 (6%)Acyl-CoA Hydrolysis 1.39E+00 1.67E-01 1/12 (8%) 1/12 (8%)Growth Hormone Signaling 1.39E+00 7.25E-02 0.447 4/69 (6%) 1/69 (1%)Type II Diabetes Mellitus Signaling 1.38E+00 5.98E-02 6/117 (5%) 1/117 (1%)Salvage Pathways of Pyrimidine Ribonucleotides 1.37E+00 6.45E-02 1/93 (1%) 5/93 (5%)PEDF Signaling 1.35E+00 7.04E-02 3/71 (4%) 2/71 (3%)Glioma Signaling 1.34E+00 6.32E-02 -0.816 4/95 (4%) 2/95 (2%)Leukocyte Extravasation Signaling 1.34E+00 5.05E-02 -1.667 8/198 (4%) 2/198 (1%)Gαi Signaling 1.33E+00 5.83E-02 0 2/120 (2%) 5/120 (4%)HMGB1 Signaling 1.33E+00 5.83E-02 -0.378 4/120 (3%) 3/120 (3%)IL-17 Signaling 1.33E+00 6.94E-02 3/72 (4%) 2/72 (3%)Endothelin-1 Signaling 1.33E+00 5.23E-02 -0.333 5/172 (3%) 4/172 (2%)JAK/Stat Signaling 1.33E+00 6.94E-02 0.447 4/72 (6%) 1/72 (1%)Role of p14/p19ARF in Tumor Suppression 1.32E+00 1.00E-01 3/30 (10%) 0/30 (0%)Oleate Biosynthesis II (Animals) 1.32E+00 1.54E-01 2/13 (15%) 0/13 (0%)LPS-stimulated MAPK Signaling 1.31E+00 6.85E-02 -0.447 3/73 (4%) 2/73 (3%)Gαq Signaling 1.31E+00 5.44E-02 -1.134 6/147 (4%) 2/147 (1%)

Table S6. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO hippocampus compared to WT controls. Significantly-populated canonical signaling pathways, generated using the transcripts significantly and differentially regulated in T1R3KO hippocampus compared to WT controls, are depicted. The pathway enrichment probability (stated as –log10(p-value)) and enrichment ratio are stated. The predicted z-score (where available), indicating a preference for ‘activation’ (positive scores) or ‘inhibition’ (negative scores) of the specific pathways are stated. The percentages of the total pathway genelist (indicated as number of experimental transcripts identified/total transcripts linked to the pathway) populated by down- (Downregulated) or upregulated (Upregulated) transcripts from the input datasets are also indicated. A percentage pathway population, by the experimentally identified transcripts is indicated in parentheses.

Ingenuity Canonical Pathways -log(p-value) Ratio z-score Downregulated Upregulated AMPK Signaling 3.53E+00 7.26E-02 -0.707 9/179 (5%) 4/179 (2%)Phospholipase C Signaling 3.30E+00 6.28E-02 0 8/239 (3%) 7/239 (3%)Ephrin Receptor Signaling 3.10E+00 6.90E-02 4/174 (2%) 8/174 (5%)Axonal Guidance Signaling 2.88E+00 4.85E-02 7/433 (2%) 14/433 (3%)Methylglyoxal Degradation I 2.80E+00 6.67E-01 1/3 (33%) 1/3 (33%)P2Y Purigenic Receptor Signaling Pathway 2.73E+00 7.56E-02 0 4/119 (3%) 5/119 (4%)HER-2 Signaling in Breast Cancer 2.71E+00 9.21E-02 4/76 (5%) 3/76 (4%)CREB Signaling in Neurons 2.65E+00 6.43E-02 0 4/171 (2%) 7/171 (4%)Acyl-CoA Hydrolysis 2.63E+00 2.50E-01 2/12 (17%) 1/12 (8%)Role of NFAT in Cardiac Hypertrophy 2.49E+00 6.15E-02 -1.265 7/179 (4%) 4/179 (2%)NRF2-mediated Oxidative Stress Response 2.47E+00 6.11E-02 -0.378 7/180 (4%) 4/180 (2%)fMLP Signaling in Neutrophils 2.43E+00 7.41E-02 0.378 3/108 (3%) 5/108 (5%)Tec Kinase Signaling 2.40E+00 6.33E-02 -0.707 5/158 (3%) 5/158 (3%)α-Adrenergic Signaling 2.38E+00 8.05E-02 -0.447 3/87 (3%) 4/87 (5%)Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis 2.36E+00 5.03E-02 8/298 (3%) 7/298 (2%)Corticotropin Releasing Hormone Signaling 2.36E+00 7.21E-02 5/111 (5%) 3/111 (3%)Virus Entry via Endocytic Pathways 2.33E+00 7.87E-02 4/89 (4%) 3/89 (3%)Glutamate Degradation III (via 4-aminobutyrate) 2.29E+00 4.00E-01 0/5 (0%) 2/5 (40%)Molecular Mechanisms of Cancer 2.26E+00 4.66E-02 12/365 (3%) 5/365 (1%)Salvage Pathways of Pyrimidine Ribonucleotides 2.22E+00 7.53E-02 2/93 (2%) 5/93 (5%)Type II Diabetes Mellitus Signaling 2.22E+00 6.84E-02 5/117 (4%) 3/117 (3%)IL-3 Signaling 2.21E+00 8.45E-02 4/71 (6%) 2/71 (3%)Prolactin Signaling 2.15E+00 8.22E-02 0 3/73 (4%) 3/73 (4%)Glioblastoma Multiforme Signaling 2.14E+00 6.16E-02 0.333 5/146 (3%) 4/146 (3%)Cholecystokinin/Gastrin-mediated Signaling 2.03E+00 6.93E-02 -1.134 5/101 (5%) 2/101 (2%)CXCR4 Signaling 2.03E+00 5.92E-02 0 5/152 (3%) 4/152 (3%)Prostate Cancer Signaling 1.92E+00 7.32E-02 3/82 (4%) 3/82 (4%)tRNA Charging 1.90E+00 1.03E-01 2/39 (5%) 2/39 (5%)ErbB4 Signaling 1.89E+00 8.33E-02 -0.447 3/60 (5%) 2/60 (3%)mTOR Signaling 1.89E+00 5.32E-02 0 6/188 (3%) 4/188 (2%)Oxidative Phosphorylation 1.86E+00 6.42E-02 2/109 (2%) 5/109 (5%)FGF Signaling 1.84E+00 7.06E-02 -1.633 5/85 (6%) 1/85 (1%)Mechanisms of Viral Exit from Host Cells 1.83E+00 9.76E-02 3/41 (7%) 1/41 (2%)ErbB Signaling 1.82E+00 6.98E-02 4/86 (5%) 2/86 (2%)Antiproliferative Role of Somatostatin Receptor 2 1.81E+00 7.94E-02 2/63 (3%) 3/63 (5%)ERK5 Signaling 1.81E+00 7.94E-02 0 3/63 (5%) 2/63 (3%)Estrogen-Dependent Breast Cancer Signaling 1.81E+00 7.94E-02 -0.447 3/63 (5%) 2/63 (3%)Pyridoxal 5'-phosphate Salvage Pathway 1.78E+00 7.81E-02 2/64 (3%) 3/64 (5%)Neuregulin Signaling 1.78E+00 6.82E-02 0.447 4/88 (5%) 2/88 (2%)

Leucine Degradation I 1.76E+00 2.22E-01 1/9 (11%) 1/9 (11%)Heme Biosynthesis II 1.76E+00 2.22E-01 1/9 (11%) 1/9 (11%)Huntington's Disease Signaling 1.73E+00 4.80E-02 4/229 (2%) 7/229 (3%)Neurotrophin/TRK Signaling 1.70E+00 7.46E-02 -0.447 3/67 (4%) 2/67 (3%)Macropinocytosis Signaling 1.68E+00 7.35E-02 0 2/68 (3%) 3/68 (4%)Synaptic Long Term Potentiation 1.67E+00 5.88E-02 4/119 (3%) 3/119 (3%)Fcγ Receptor-mediated Phagocytosis in Macrophages and Monocytes 1.67E+00 6.45E-02 0 3/93 (3%) 3/93 (3%)CCR5 Signaling in Macrophages 1.65E+00 7.25E-02 2/69 (3%) 3/69 (4%)Growth Hormone Signaling 1.65E+00 7.25E-02 -0.447 3/69 (4%) 2/69 (3%)B Cell Receptor Signaling 1.65E+00 5.11E-02 5/176 (3%) 4/176 (2%)Glioma Signaling 1.63E+00 6.32E-02 -0.447 4/95 (4%) 2/95 (2%)PPARα/RXRα Activation 1.61E+00 5.03E-02 -0.378 6/179 (3%) 3/179 (2%)JAK/Stat Signaling 1.58E+00 6.94E-02 -0.447 3/72 (4%) 2/72 (3%)LPS-stimulated MAPK Signaling 1.56E+00 6.85E-02 -0.447 3/73 (4%) 2/73 (3%)NF-κB Activation by Viruses 1.56E+00 6.85E-02 0.447 2/73 (3%) 3/73 (4%)STAT3 Pathway 1.56E+00 6.85E-02 3/73 (4%) 2/73 (3%)Gap Junction Signaling 1.54E+00 5.16E-02 6/155 (4%) 2/155 (1%)Estrogen Receptor Signaling 1.54E+00 5.51E-02 4/127 (3%) 3/127 (2%)Clathrin-mediated Endocytosis Signaling 1.53E+00 4.86E-02 7/185 (4%) 2/185 (1%)GNRH Signaling 1.50E+00 5.43E-02 -0.378 4/129 (3%) 3/129 (2%)Hereditary Breast Cancer Signaling 1.50E+00 5.43E-02 4/129 (3%) 3/129 (2%)Oleate Biosynthesis II (Animals) 1.45E+00 1.54E-01 1/13 (8%) 1/13 (8%)Cholesterol Biosynthesis I 1.45E+00 1.54E-01 1/13 (8%) 1/13 (8%)Cholesterol Biosynthesis II (via 24,25-dihydrolanosterol) 1.45E+00 1.54E-01 1/13 (8%) 1/13 (8%)Cholesterol Biosynthesis III (via Desmosterol) 1.45E+00 1.54E-01 1/13 (8%) 1/13 (8%)HGF Signaling 1.44E+00 5.71E-02 -0.816 4/105 (4%) 2/105 (2%)Human Embryonic Stem Cell Pluripotency 1.43E+00 5.22E-02 3/134 (2%) 4/134 (3%)Thrombopoietin Signaling 1.41E+00 7.27E-02 0 2/55 (4%) 2/55 (4%)NGF Signaling 1.41E+00 5.61E-02 0 3/107 (3%) 3/107 (3%)Circadian Rhythm Signaling 1.39E+00 9.09E-02 1/33 (3%) 2/33 (6%)Fc Epsilon RI Signaling 1.38E+00 5.50E-02 -0.816 4/109 (4%) 2/109 (2%)ErbB2-ErbB3 Signaling 1.36E+00 7.02E-02 3/57 (5%) 1/57 (2%)Natural Killer Cell Signaling 1.36E+00 5.45E-02 3/110 (3%) 3/110 (3%)Type I Diabetes Mellitus Signaling 1.36E+00 5.45E-02 4/110 (4%) 2/110 (2%)Androgen Signaling 1.35E+00 5.41E-02 2/111 (2%) 4/111 (4%)Role of NANOG in Mammalian Embryonic Stem Cell Pluripotency 1.35E+00 5.41E-02 3/111 (3%) 3/111 (3%)phagosome formation 1.35E+00 5.41E-02 4/111 (4%) 2/111 (2%)Melanocyte Development and Pigmentation Signaling 1.34E+00 5.95E-02 -0.447 3/84 (4%) 2/84 (2%)Mitochondrial Dysfunction 1.33E+00 4.68E-02 2/171 (1%) 6/171 (4%)Role of NFAT in Regulation of the Immune Response 1.33E+00 4.68E-02 -0.816 4/171 (2%) 4/171 (2%)Cell Cycle Regulation by BTG Family Proteins 1.33E+00 8.57E-02 2/35 (6%) 1/35 (3%)eNOS Signaling 1.32E+00 4.93E-02 -0.447 5/142 (4%) 2/142 (1%)RhoGDI Signaling 1.30E+00 4.62E-02 4/173 (2%) 4/173 (2%)NF-κB Signaling 1.30E+00 4.62E-02 -0.707 6/173 (3%) 2/173 (1%)Colorectal Cancer Metastasis Signaling 1.30E+00 4.24E-02 -0.333 5/236 (2%) 5/236 (2%)Stearate Biosynthesis I (Animals) 1.30E+00 8.33E-02 2/36 (6%) 1/36 (3%)

Table S7. Canonical Pathway Signaling analysis for transcripts differentially regulated in T1R3KO hypothalamus compared to WT controls. Significantly-populated canonical signaling pathways, generated using the transcripts significantly and differentially regulated in T1R3KO hypothalamus compared to WT controls, are depicted. The pathway enrichment probability (stated as –log10(p-value)) and enrichment ratio are stated. The predicted z-score (where available), indicating a preference for ‘activation’ (positive scores) or ‘inhibition’ (negative scores) of the specific pathways are stated. The percentages of the total pathway genelist (indicated as number of experimental transcripts identified/total transcripts linked to the pathway) populated by down- (Downregulated) or upregulated (Upregulated) transcripts from the input datasets are also indicated. A percentage pathway population, by the experimentally identified transcripts is indicated in parentheses.

Ingenuity Canonical Pathways -log(p-value) Ratio z-score Downregulated Upregulated fMLP Signaling in Neutrophils 3.16E+00 7.41E-02 0.378 3/108 (3%) 5/108 (5%)Melanocyte Development and Pigmentation Signaling 3.13E+00 8.33E-02 1.134 2/84 (2%) 5/84 (6%)Antiproliferative Role of Somatostatin Receptor 2 3.06E+00 9.52E-02 0.447 2/63 (3%) 4/63 (6%)G Beta Gamma Signaling 3.01E+00 7.95E-02 1/88 (1%) 6/88 (7%)CREB Signaling in Neurons 3.01E+00 5.85E-02 1.414 2/171 (1%) 8/171 (5%)Neurotrophin/TRK Signaling 2.92E+00 8.96E-02 0.816 2/67 (3%) 4/67 (6%)P2Y Purigenic Receptor Signaling Pathway 2.89E+00 6.72E-02 1.134 2/119 (2%) 6/119 (5%)Gαi Signaling 2.87E+00 6.67E-02 3/120 (3%) 5/120 (4%)phagosome maturation 2.87E+00 6.67E-02 3/120 (3%) 5/120 (4%)Mouse Embryonic Stem Cell Pluripotency 2.82E+00 7.37E-02 -0.378 4/95 (4%) 3/95 (3%)NF-κB Activation by Viruses 2.73E+00 8.22E-02 0.816 2/73 (3%) 4/73 (5%)FLT3 Signaling in Hematopoietic Progenitor Cells 2.70E+00 8.11E-02 2/74 (3%) 4/74 (5%)CNTF Signaling 2.65E+00 9.62E-02 0.447 2/52 (4%) 3/52 (6%)Breast Cancer Regulation by Stathmin1 2.65E+00 5.24E-02 3/191 (2%) 7/191 (4%)IL-2 Signaling 2.61E+00 9.43E-02 0.447 2/53 (4%) 3/53 (6%)G Protein Signaling Mediated by Tubby 2.58E+00 1.21E-01 0/33 (0%) 4/33 (12%)Oncostatin M Signaling 2.53E+00 1.18E-01 0 2/34 (6%) 2/34 (6%)Glioma Invasiveness Signaling 2.47E+00 8.77E-02 0.447 3/57 (5%) 2/57 (4%)Role of Tissue Factor in Cancer 2.46E+00 6.36E-02 2/110 (2%) 5/110 (5%)Role of NFAT in Regulation of the Immune Response 2.45E+00 5.26E-02 1.134 2/171 (1%) 7/171 (4%)autophagy 2.44E+00 1.11E-01 2/36 (6%) 2/36 (6%)Phospholipase C Signaling 2.42E+00 4.60E-02 1.414 3/239 (1%) 8/239 (3%)Ephrin Receptor Signaling 2.40E+00 5.17E-02 2/174 (1%) 7/174 (4%)Antigen Presentation Pathway 2.39E+00 1.08E-01 3/37 (8%) 1/37 (3%)α-Adrenergic Signaling 2.34E+00 6.90E-02 1 1/87 (1%) 5/87 (6%)Epithelial Adherens Junction Signaling 2.34E+00 5.48E-02 4/146 (3%) 4/146 (3%)GM-CSF Signaling 2.32E+00 8.06E-02 0.447 2/62 (3%) 3/62 (5%)CCR3 Signaling in Eosinophils 2.31E+00 5.98E-02 2/117 (2%) 5/117 (4%)tRNA Charging 2.31E+00 1.03E-01 1/39 (3%) 3/39 (8%)Virus Entry via Endocytic Pathways 2.30E+00 6.74E-02 4/89 (4%) 2/89 (2%)Estrogen-Dependent Breast Cancer Signaling 2.29E+00 7.94E-02 0.447 2/63 (3%) 3/63 (5%)Thyroid Cancer Signaling 2.27E+00 1.00E-01 2/40 (5%) 2/40 (5%)IL-8 Signaling 2.24E+00 4.89E-02 1.414 2/184 (1%) 7/184 (4%)Regulation of the Epithelial-Mesenchymal Transition Pathway 2.24E+00 4.89E-02 4/184 (2%) 5/184 (3%)Systemic Lupus Erythematosus Signaling 2.21E+00 4.55E-02 4/220 (2%) 6/220 (3%)IL-15 Signaling 2.20E+00 7.58E-02 2/66 (3%) 3/66 (5%)Melanoma Signaling 2.19E+00 9.52E-02 2/42 (5%) 2/42 (5%)PEDF Signaling 2.07E+00 7.04E-02 3/71 (4%) 2/71 (3%)Ovarian Cancer Signaling 2.05E+00 5.34E-02 4/131 (3%) 3/131 (2%)

Ephrin B Signaling 2.02E+00 6.85E-02 0/73 (0%) 5/73 (7%)Leucine Degradation I 1.98E+00 2.22E-01 1/9 (11%) 1/9 (11%)Heme Biosynthesis II 1.98E+00 2.22E-01 1/9 (11%) 1/9 (11%)PDGF Signaling 1.92E+00 6.49E-02 0.447 2/77 (3%) 3/77 (4%)NGF Signaling 1.92E+00 5.61E-02 0.816 2/107 (2%) 4/107 (4%)Acute Myeloid Leukemia Signaling 1.88E+00 6.33E-02 -0.447 3/79 (4%) 2/79 (3%)Endometrial Cancer Signaling 1.87E+00 7.69E-02 2/52 (4%) 2/52 (4%)Natural Killer Cell Signaling 1.86E+00 5.45E-02 2/110 (2%) 4/110 (4%)Dendritic Cell Maturation 1.83E+00 4.47E-02 4/179 (2%) 4/179 (2%)Role of NFAT in Cardiac Hypertrophy 1.83E+00 4.47E-02 1.134 2/179 (1%) 6/179 (3%)Prostate Cancer Signaling 1.81E+00 6.10E-02 2/82 (2%) 3/82 (4%)Thrombopoietin Signaling 1.79E+00 7.27E-02 0 2/55 (4%) 2/55 (4%)G-Protein Coupled Receptor Signaling 1.78E+00 3.91E-02 4/256 (2%) 6/256 (2%)Fatty Acid β-oxidation I 1.76E+00 9.68E-02 2/31 (6%) 1/31 (3%)FGF Signaling 1.75E+00 5.88E-02 1.342 1/85 (1%) 4/85 (5%)14-3-3-mediated Signaling 1.74E+00 5.13E-02 3/117 (3%) 3/117 (3%)ErbB2-ErbB3 Signaling 1.73E+00 7.02E-02 2/57 (4%) 2/57 (4%)Heparan Sulfate Biosynthesis 1.73E+00 7.02E-02 3/57 (5%) 1/57 (2%)Acyl-CoA Hydrolysis 1.73E+00 1.67E-01 1/12 (8%) 1/12 (8%)ErbB Signaling 1.73E+00 5.81E-02 2/86 (2%) 3/86 (3%)Cytotoxic T Lymphocyte-mediated Apoptosis of Target Cells 1.73E+00 9.38E-02 2/32 (6%) 1/32 (3%)PKCθ Signaling in T Lymphocytes 1.73E+00 5.08E-02 2/118 (2%) 4/118 (3%)CXCR4 Signaling 1.72E+00 4.61E-02 0.816 2/152 (1%) 5/152 (3%)mTOR Signaling 1.71E+00 4.26E-02 0.378 4/188 (2%) 4/188 (2%)Bladder Cancer Signaling 1.71E+00 5.75E-02 1/87 (1%) 4/87 (5%)Synaptic Long Term Potentiation 1.71E+00 5.04E-02 1/119 (1%) 5/119 (4%)Myc Mediated Apoptosis Signaling 1.71E+00 6.90E-02 3/58 (5%) 1/58 (2%)Neuregulin Signaling 1.69E+00 5.68E-02 1.342 2/88 (2%) 3/88 (3%)UVA-Induced MAPK Signaling 1.69E+00 5.68E-02 0.447 2/88 (2%) 3/88 (3%)Thrombin Signaling 1.68E+00 4.19E-02 0.816 2/191 (1%) 6/191 (3%)Crosstalk between Dendritic Cells and Natural Killer Cells 1.67E+00 5.62E-02 3/89 (3%) 2/89 (2%)Apoptosis Signaling 1.67E+00 5.62E-02 -1.342 2/89 (2%) 3/89 (3%)ErbB4 Signaling 1.66E+00 6.67E-02 0 2/60 (3%) 2/60 (3%)Signaling by Rho Family GTPases 1.61E+00 3.85E-02 -0.816 3/234 (1%) 6/234 (3%)Colorectal Cancer Metastasis Signaling 1.59E+00 3.81E-02 0.707 3/236 (1%) 6/236 (3%)ERK5 Signaling 1.59E+00 6.35E-02 1 1/63 (2%) 3/63 (5%)Role of JAK1 and JAK3 in γc Cytokine Signaling 1.59E+00 6.35E-02 2/63 (3%) 2/63 (3%)SAPK/JNK Signaling 1.58E+00 5.32E-02 0.447 2/94 (2%) 3/94 (3%)Glioma Signaling 1.57E+00 5.26E-02 0.447 2/95 (2%) 3/95 (3%)GNRH Signaling 1.56E+00 4.65E-02 1.633 1/129 (1%) 5/129 (4%)Non-Small Cell Lung Cancer Signaling 1.55E+00 6.15E-02 0 2/65 (3%) 2/65 (3%)T Cell Receptor Signaling 1.53E+00 5.15E-02 2/97 (2%) 3/97 (3%)Angiopoietin Signaling 1.53E+00 6.06E-02 3/66 (5%) 1/66 (2%)Erythropoietin Signaling 1.51E+00 5.97E-02 2/67 (3%) 2/67 (3%)CDK5 Signaling 1.50E+00 5.05E-02 0.447 2/99 (2%) 3/99 (3%)GDNF Family Ligand-Receptor Interactions 1.49E+00 5.88E-02 0 2/68 (3%) 2/68 (3%)Neuroprotective Role of THOP1 in Alzheimer's Disease 1.47E+00 7.50E-02 2/40 (5%) 1/40 (3%)Endothelin-1 Signaling 1.47E+00 4.07E-02 -0.378 4/172 (2%) 3/172 (2%)Agrin Interactions at Neuromuscular Junction 1.47E+00 5.80E-02 1/69 (1%) 3/69 (4%)Role of MAPK Signaling in the Pathogenesis of Influenza 1.47E+00 5.80E-02 2/69 (3%) 2/69 (3%)HIF1α Signaling 1.45E+00 4.90E-02 3/102 (3%) 1/2 (50%)FcγRIIB Signaling in B Lymphocytes 1.44E+00 7.32E-02 2/41 (5%) 2/102 (2%)IL-3 Signaling 1.43E+00 5.63E-02 2/71 (3%) 1/41 (2%)

Renal Cell Carcinoma Signaling 1.43E+00 5.63E-02 0 2/71 (3%) 2/71 (3%)Chemokine Signaling 1.43E+00 5.63E-02 1 1/71 (1%) 2/71 (3%)Rac Signaling 1.42E+00 4.81E-02 -0.447 3/104 (3%) 3/71 (4%)B Cell Receptor Signaling 1.42E+00 3.98E-02 2/176 (1%) 2/104 (2%)UVC-Induced MAPK Signaling 1.42E+00 7.14E-02 1/42 (2%) 5/176 (3%)IL-17 Signaling 1.41E+00 5.56E-02 2/72 (3%) 2/42 (5%)JAK/Stat Signaling 1.41E+00 5.56E-02 0 2/72 (3%) 2/72 (3%)Actin Cytoskeleton Signaling 1.39E+00 3.69E-02 4/217 (2%) 2/72 (3%)LPS-stimulated MAPK Signaling 1.39E+00 5.48E-02 0 2/73 (3%) 4/217 (2%)Prolactin Signaling 1.39E+00 5.48E-02 0 2/73 (3%) 2/73 (3%)STAT3 Pathway 1.39E+00 5.48E-02 2/73 (3%) 2/73 (3%)Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis 1.39E+00 3.36E-02 3/298 (1%) 2/73 (3%)Role of IL-17F in Allergic Inflammatory Airway Diseases 1.37E+00 6.82E-02 0/44 (0%) 7/298 (2%)Renin-Angiotensin Signaling 1.35E+00 4.59E-02 0 2/109 (2%) 3/44 (7%)Gαs Signaling 1.35E+00 4.59E-02 2 0/109 (0%) 3/109 (3%)Cardiac Hypertrophy Signaling 1.34E+00 3.59E-02 1.134 2/223 (1%) 5/109 (5%)HER-2 Signaling in Breast Cancer 1.34E+00 5.26E-02 3/76 (4%) 6/223 (3%)VEGF Family Ligand-Receptor Interactions 1.34E+00 5.26E-02 0 2/76 (3%) 1/76 (1%)Gαq Signaling 1.32E+00 4.08E-02 1.342 2/147 (1%) 2/76 (3%)EIF2 Signaling 1.32E+00 3.78E-02 0.447 3/185 (2%) 4/147 (3%)Role of NANOG in Mammalian Embryonic Stem Cell Pluripotency 1.32E+00 4.50E-02 0 2/111 (2%) 4/185 (2%)Granzyme A Signaling 1.32E+00 1.00E-01 1/20 (5%) 3/111 (3%)

Table S8. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO cortex compared to WT controls. For each specified BioFunction/Disease annotation significantly populated the probability of enrichment (scored as –log10(p-value)) is stated along with the predicted activation state (Increased or Decreased), activation z-score (positive indicates activation and negative indicates inhibition) and number (#) of experimentally-derived input transcripts falling into the specific BioFunction/Disease pathway.

Diseases or Functions Annotation -log10(p-value) Activation State Activation z-score # Transcripts

morphology of nervous tissue 9.342944 44

morphology of nervous system 8.847712 64

Movement Disorders 8.818156 1.843 79

morphology of neurons 8.8041 42

development of neurons 7.415669 1.561 59

abnormal morphology of nervous system 7.170696 56

formation of plasma membrane projections 6.954677 1.824 48

organization of cytoskeleton 6.924453 0.521 90

abdominal neoplasm 6.832683 1.368 407

organization of cytoplasm 6.759451 0.531 96

abdominal cancer 6.500313 1.159 402

morphology of neurites 6.49485 19neuritogenesis 6.465974 Increased 2.082 46

formation of cellular protrusions 6.340084 0.725 63

morphology of cells 6.232844 115

abnormal morphology of neurons 6.178486 33

disorder of basal ganglia 5.931814 55

neurological signs 5.735182 0.447 48

tumorigenesis of tissue 5.679854 1.155 410

microtubule dynamics 5.605548 0.423 75

neoplasia of epithelial tissue 5.431798 0.98 403

abnormal morphology of neurites 5.390406 15

long-term potentiation 5.36251 0.807 23exocytosis 5.29073 Decreased -2.166 19

neuromuscular disease 5.28735 59

cell death 5.268411 -0.464 173

glioma cancer 5.21254 23

epithelial cancer 5.167491 1.208 399

Huntington's Disease 5.076238 42

plasticity of synapse 4.995679 0.277 14

dyskinesia 4.982967 44

behavior 4.939302 -0.317 64

digestive organ tumor 4.869666 0.549 348

abnormal morphology of cells 4.869666 77abnormal morphology of plasma membrane 4.866461 18

female genital tract serous cancer 4.847712 27

abnormal morphology of head 4.8041 55

digestive system cancer 4.692504 0.902 345

candidemia 4.692504 3

morphology of dendrites 4.605548 11

apoptosis 4.477556 -0.445 138

long-term potentiation of brain 4.476254 -0.066 14

abnormal morphology of axons 4.465974 11

internalization of fatty acid 4.449772 4

abnormal morphology of brain 4.44855 33

morphology of head 4.442493 56abnormal morphology of central nervous system 4.404504 35

morphology of brain 4.402305 34long-term potentiation of hippocampal CA1 region 4.30103 0.293 8

uterine serous papillary cancer 4.25649 20

morphogenesis of neurons 4.243364 1.649 31

exocytosis by cells 4.230623 -0.864 13

febrile seizure 4.176526 7

morphology of central nervous system 4.175874 36autosomal recessive Charcot-Marie-Tooth disease type 2 4.098542 3

ataxia 4.041914 1.588 21

glioblastoma cancer 4.037157 20

morphogenesis of neurites 3.9914 1.649 30

long-term potentiation of hippocampus 3.982967 0.33 12

cognition 3.978811 -0.944 32

seizures 3.946922 1.653 29

abdominal adenocarcinoma 3.853872 274

transcription 3.832683 1.325 105

amyloidosis 3.829738 38

necrosis 3.785156 -0.586 131autosomal dominant epilepsy with febrile seizures plus 3.709965 3

growth of corticospinal axon 3.709965 3

growth of raphespinal axon 3.709965 3

seizure disorder 3.694649 1.654 32neurotransmission 3.669586 Decreased -2.137 29

serous neoplasm 3.643974 37

neurodegeneration of neurites 3.642065 0.771 10

Alzheimer's disease 3.634512 36

transcription of RNA 3.630784 1.728 98organismal death 3.621602 Increased 2.032 122

excitatory postsynaptic potential 3.619789 12

Rac protein signal transduction 3.617983 6

learning 3.614394 -1.126 29

gastrointestinal carcinoma 3.59176 217

neuronal cell death 3.585027 -0.876 43

endocytosis of protein 3.570248 -0.218 5

porphyria 3.488117 4

idiopathic generalized epilepsy 3.473661 5

internalization of lipid 3.473661 5

cleavage of acyl-coenzyme A 3.416801 3central nervous system tumor 3.402305 Increased 2.183 51

quantity of cells 3.400117 -1.481 94

gastrointestinal adenocarcinoma 3.383 205

hyperplasia of hematopoietic progenitor cells 3.381952 0 5

inhibition of neurons 3.381952 -0.152 5

synaptic transmission 3.357535 -1.463 24

gastrointestinal tract cancer 3.336299 0.655 237

Gastrointestinal Tract Cancer and Tumors 3.330683 -0.479 239

dendritic growth/branching 3.316053 1.813 17

peroxisomal disorder 3.294992 5

nurture 3.272459 6

central nervous system cancer 3.270835 46

apoptosis of neurons 3.242604 -0.571 29

neuroepithelial tumor 3.229885 1.455 45

potentiation of synapse 3.226945 1.585 13

Rasmussen's encephalitis 3.226214 4

branching of cerebral cortex cells 3.226214 1.982 4

extension of plasma membrane projections 3.221126 1.549 15

quantity of neurons 3.216096 -0.385 28

neurodegeneration of axons 3.208309 1.08 9

epileptic seizure 3.20621 1 14

guanine nucleotide exchange of GTP 3.183096 3

expression of RNA 3.166216 1.625 108

demyelination 3.159894 1.143 11

transitional cell epithelial cancer 3.134304 12

Schizophrenia 3.133122 33

development of central nervous system 3.128427 -1.109 39

G0/G1 phase transition of fibroblasts 3.126679 2

Hutchinson-Gilford progeria syndrome 3.126679 2

anal squamous cell cancer 3.126679 2

degradation of 2-arachidonoylglycerol 3.126679 2

degradation of anandamide 3.126679 2

development of endoderm progenitors 3.126679 2

inhibition of dorsal root ganglion cells 3.126679 2

quantity of alpha granules 3.126679 2

recruitment of synaptic vesicles 3.126679 2

regulation of 2-arachidonoylglycerol 3.126679 2

severe myoclonic epilepsy borderline 3.126679 2

specification of neuronal progenitor cells 3.126679 2

thickness of optic nerve 3.126679 2

Zellweger syndrome 3.110698 4

function of oligodendrocytes 3.110698 4

morphology of brain cells 3.102373 11

malignant solid tumor 3.097453 1.299 463

formation of dendrites 3.080922 13

extension of neurites 3.070581 1.866 14

gliomatosis 3.06148 1.953 44

cancer 3.041914 1.827 468

shape change of neurites 3.009217 1.34 20

ruffling 2.995679 -0.239 10

autosomal dominant mental retardation 2.982967 5

astrocytoma 2.978811 37

demyelination of nerves 2.978811 0.896 6

branching of neurons 2.962574 1.519 20

excitatory postsynaptic current 2.958607 8

function of central nervous system 2.958607 8

metabolism of porphyrin 2.924453 6

gastroesophageal adenocarcinoma 2.906578 72

cell movement 2.879426 0.113 106

degeneration of nervous system 2.869666 -0.267 20

perinatal death 2.853872 -0.138 36

emotional behavior 2.853872 0.326 18

childhood absence epilepsy 2.821023 3

chronic granulomatous disease 2.821023 3

homeostasis of metal ion 2.818156 14

Charcot-Marie-Tooth disease type 2 2.809668 4

organization of peroxisomes 2.809668 4

major depression 2.798603 14

demyelinating peripheral neuropathy 2.790485 7

branching of cells 2.787812 0.9 24

cell death of connective tissue cells 2.787812 0.347 36

cell death of fibroblast cell lines 2.782516 0.355 27

branching of neurites 2.782516 1.333 19

long-term potentiation of synapse 2.769551 1.36 12

gastro-esophageal carcinoma 2.747147 75

lichen planus 2.744727 9

allergic pulmonary eosinophilia 2.723538 5

status epilepticus 2.723538 5

internalization of protein 2.714443 -0.186 9

pelvic cancer 2.707744 201

pelvic tumor 2.679854 202

branching morphogenesis of lung 2.675718 3

branching of nerves 2.675718 3

formation of peroxisomes 2.675718 3

genital tumor 2.67162 188

cleavage of fatty acid 2.66354 -0.809 5

Charcot-Marie-Tooth disease type 2A 2.657577 2

co-localization of blood cells 2.657577 2

conduction block 2.657577 2

dysplasia of exocrine cells 2.657577 2

function of vestibular hair cells 2.657577 2

generation of cortical interneurons 2.657577 2

infection of peritoneal macrophages 2.657577 2

internalization of eicosanoid 2.657577 2

long-term potentiation of mossy fiber cells 2.657577 2

lung carcinoid tumor 2.657577 2

lung sarcomatoid carcinoma 2.657577 2progressive experimental autoimmune encephalomyelitis 2.657577 2

sporadic adenoma 2.657577 2

anaplastic glioma 2.640165 4

chemically-elicited antinociception 2.640165 1.067 4

morphology of central nervous system cells 2.636388 12

axonogenesis 2.616185 1.506 16

mucinous neoplasm 2.605548 5

length of plasma membrane projections 2.581699 -0.128 8

epilepsy 2.570248 1.342 20

migration of cells 2.567031 -0.789 94

maturation of intercellular junctions 2.562249 4metabolism of nucleic acid component or derivative 2.55752 -0.26 31

heart conduction disorder 2.551294 5abnormal morphology of double-positive thymocyte 2.545155 3

dysplasia of cells 2.545155 3quantity of phosphatidylinositol 3,4-diphosphate 2.545155 3

Parkinson's disease 2.543634 17

migration of neurons 2.528708 0.158 17

transport of molecule 2.521434 -0.857 79

depressive disorder 2.514279 17bodily balance 2.50307 Decreased -2.219 6

Lennox-Gastaut syndrome 2.497573 5

maternal nurturing 2.497573 5

intestinal polyposis 2.489455 4

redistribution of lipid 2.489455 1.982 4

activation of Protein kinase 2.485452 18

upper gastrointestinal tract cancer 2.484126 87

differentiation of cells 2.467246 0.323 109

Neurodegeneration 2.463442 -0.267 20

organization of cell-cell contacts 2.455932 7

colorectal carcinoma 2.44855 173

development of epithelial cell lines 2.446117 -0.277 5

size of muscle cells 2.441291 10

apoptosis of brain cells 2.438899 -0.832 11

Encephalitis 2.438899 -0.497 19

cell death of brain 2.438899 -0.578 19abnormal morphology of cerebral ventricles 2.438899 9

gastric adenocarcinoma 2.436519 59

gastric carcinoma 2.430626 61

differentiation of muscle cell lines 2.430626 0.447 12

cell death of brain cells 2.429457 -0.291 18

abnormal auditory evoked potential 2.429457 3

apoptosis of retinal ganglion cells 2.429457 3

damage of aorta 2.429457 3

import of lipid 2.429457 3

priming of synaptic vesicles 2.429457 3

spike-wave discharge 2.429457 3

thickness of cerebral cortex 2.429457 3

cell death of central nervous system cells 2.424812 -0.079 19

formation of cellular membrane 2.424812 -0.755 17

organization of organelle 2.423659 31

migration of neuroblasts 2.419075 -1.091 4

homeostasis of Ca2+ 2.417937 10

long term depression 2.412289 -0.346 9

morphology of cerebral cortex 2.411168 14

myelination 2.411168 0.184 14

shape change of axons 2.402305 0.911 8

gastroesophageal cancer 2.400117 79

homeostasis of divalent cations 2.396856 11

vocalization 2.396856 5

length of neurites 2.38934 -0.555 7

G2/M phase of carcinoma cell lines 2.364516 2

acinar to ductal metaplasia 2.364516 2

area of neointima 2.364516 2

development of GABAergic neurons 2.364516 2

differentiation of projection neurons 2.364516 2entry into S phase of hematopoietic progenitor cells 2.364516 2

episodic ataxia 2.364516 2

hydrolysis of ADP 2.364516 2

hydrolysis of glycerol 2.364516 2

import of fatty acid 2.364516 2

internalization of long chain fatty acid 2.364516 2

interphase of vascular smooth muscle cells 2.364516 2

lack of tunnel of Corti 2.364516 2

mixed Mullerian endometrial cancer 2.364516 2

serrated adenoma 2.364516 2

upper gastrointestinal tract tumor 2.361511 89

urogenital cancer 2.355561 1.026 203

tumorigenesis of genital organ 2.355561 180

apoptosis of sensory neurons 2.353596 -1 4

object recognition memory 2.353596 4

organization of synapse 2.347754 5

adenocarcinoma 2.342944 0.359 290

G2/M phase transition of fibroblasts 2.324222 3

bobbing of head 2.324222 3

colorectal polyposis 2.324222 3

quantity of plasma membrane projections 2.317855 -1.463 8

sensory neuropathy 2.317855 8

genital tract cancer 2.313364 178

abnormal morphology of cerebral cortex 2.308919 13

colorectal adenocarcinoma 2.308919 168

organization of nervous tissue 2.306273 6

ingestion of ethanol 2.30103 -1.342 5

morphology of membranous labyrinth 2.30103 5

recovery of mice 2.30103 5

progressive motor neuropathy 2.300162 30

migration of brain cells 2.29073 -0.728 8

specification of neurons 2.289883 4

endocytosis 2.283162 -1.216 21abnormal morphology of lateral cerebral ventricle 2.269218 6

G2 phase 2.269218 -0.577 17

gliosis 2.262807 -0.814 10

synaptic depression 2.262807 -0.655 10

antinociception 2.255707 1.536 5

closure of neural tube 2.241088 10

organization of cellular membrane 2.241088 10

pervasive developmental disorder 2.240332 13

social behavior 2.238072 -1.741 8

long term depression of synapse 2.233587 6

mean corpuscular volume 2.233587 -0.114 6

accumulation of glycogen 2.229885 -1.067 4

synthesis of heme 2.229885 4

S phase of epithelial cells 2.228413 3

hypersensitive reaction of ear 2.228413 3

production of male germ cells 2.228413 3

size of growth cone 2.228413 3

Charcot-Marie-Tooth disease 2.211832 5endocytosis of vesicles 2.211832 Decreased -2 5

organization of plasma membrane 2.211832 8

sprouting 2.209012 1.133 23

benign neoplasia 2.204815 1.533 43

cellular homeostasis 2.204815 -1.627 75

early-onset neurological disorder 2.198596 6

size of cells 2.193142 25

abnormal morphology of telencephalon 2.189096 12

mental retardation 2.184422 14

Mood Disorders 2.183759 26

peripheral neuropathy 2.178486 11

shape change of tumor cell lines 2.178486 0.437 11

synaptic transmission of cells 2.177178 -1.463 16

prepulse inhibition 2.173925 -1.982 7arrest in G1 phase of breast cancer cell lines 2.171985 4

autosomal recessive peripheral neuropathy 2.171985 4

autosomal recessive polyneuropathy 2.171985 4

cardiovascular disorder of cardiac valve 2.171985 4

hyperpolarization of membrane 2.171985 4

valvular regurgitation 2.171985 4

myoclonus 2.169411 5

nociception 2.154282 -0.447 12

angiogenesis of carcinoma 2.150581 2

attraction of hematopoietic progenitor cells 2.150581 2

beta-oxidation of very long chain fatty acid 2.150581 2

branching morphogenesis of nerves 2.150581 2

degeneration of optic nerve 2.150581 2

development of gonadal tumor 2.150581 2

epidermal nevus 2.150581 2

hydrolysis of arachidonic acid 2.150581 2

juvenile myoclonic epilepsy 2.150581 2

lack of stapedial artery 2.150581 2

lethargic behavior 2.150581 2

metabolism of UDP-N-acetyl-D-galactosamine 2.150581 2

metabolism of dolichol 2.150581 2

metabolism of protoporphyrinogen 2.150581 2

quantity of brain cancer cell lines 2.150581 2

regulation of dendritic cells 2.150581 2

survival of mesangial cells 2.150581 2

tumorigenesis of thymic lymphoma 2.150581 2

benign neoplasm of female genital organ 2.150581 22

transport of Ca2+ 2.150581 9

formation of plasma membrane 2.149967 -0.755 16

branching of axons 2.145087 0.861 7

maturation of neurons 2.145087 -0.651 7abnormal morphology of stria vascularis of cochlea 2.140261 3metabolism of nucleoside diphosphate sugar 2.140261 3

quantity of excitatory synapses 2.140261 3

senescence of tumor cells 2.140261 3

survival of glomerular cells 2.140261 3

multiple congenital anomalies 2.133713 24

oligodendroglioma 2.116907 4

gastric cancer 2.110138 65

mineralization of bone 2.104577 0.277 11

colorectal neoplasia 2.101824 195

transmembrane potential 2.097997 -0.42 17

proliferation of lymphoblastoid cell lines 2.097453 6

progressive neurological disorder 2.0846 -0.52 9

autosomal recessive disease 2.07263 44

oligomerization of protein 2.071604 15

size of body 2.070581 -1.927 45

convulsion 2.063989 4

quantity of astrocytes 2.063989 1.98 4

hyperactive behavior 2.059484 1.82 10

quantity of tumor cell lines 2.059484 -1.154 10

abnormal morphology of endocardium 2.058489 3

bullous pemphigoid 2.058489 3

elongation of filaments 2.058489 3

hyperpigmentation 2.058489 3

metabolism of cyclic GMP 2.058489 3

proliferation of peroxisomes 2.058489 3

contraction of heart 2.053057 -0.669 12

hypoactivity of mice 2.05061 11

size of brain 2.05061 0.923 11

organic aciduria 2.048177 5

proliferation of neuronal cells 2.036684 0.864 32

abnormal morphology of neuroglia 2.035269 7

autism 2.035269 7

memory 2.030584 -1.205 16

transport of metal 2.019542 -0.562 19

docking of vesicles 2.013228 4

heart block 2.013228 4

mucinous adenocarcinoma 2.013228 4

tumorigenesis of glioma 2.013228 4

nausea and vomiting 2.010105 5

organization of actin cytoskeleton 2.007889 18

binding of GTP 2.003051 1.131 6

quantity of synapse 2.003051 0.965 6regeneration of neurites 2.003051 Increased 2.213 6

breast or colorectal cancer 1.9914 222

cell transformation 1.987163 -0.437 26proliferation of fibroblast cell lines 1.987163 Increased 2.206 24

accumulation of lipid 1.982967 -1.116 18

cell spreading of cervical cancer cell lines 1.982967 3

condensation of cells 1.982967 3

destabilization of microtubules 1.982967 3transmembrane receptor protein serine/threonine kinase signaling pathway 1.982967 3G1/S phase transition of smooth muscle cells 1.982967 2

S phase of epidermal cells 1.982967 2

abnormal morphology of arteriole 1.982967 2

beta-oxidation of lignoceric acid 1.982967 2

branching of cortical neurons 1.982967 2

colony formation of carcinoma cells 1.982967 2colony formation of kidney cancer cell lines 1.982967 2

development of ganglion cell layer 1.982967 2

development of subpallium 1.982967 2

formation of GW body 1.982967 2

formation of joint 1.982967 2

hepatic porphyria 1.982967 2

hydrolysis of 2-arachidonoylglycerol 1.982967 2

hydrolysis of long chain fatty acid 1.982967 2

hypomyelination of axons 1.982967 2

influx of chloride 1.982967 2

iron overload of liver 1.982967 2

lack of metanephros 1.982967 2

lack of palatine processes 1.982967 2

loss of retinal ganglion cells 1.982967 2

low grade bladder tumor 1.982967 2proliferation of invariant natural killer T cells 1.982967 2

quantity of GW body 1.982967 2

redistribution of phosphatidic acid 1.982967 2

colorectal cancer 1.982967 193

diffuse large B-cell lymphoma 1.982967 11

autophagy of cells 1.978811 -0.648 16

degeneration of neurons 1.978811 -0.084 15

abnormal morphology of dendrites 1.974694 5

long-term potentiation of brain cells 1.974694 5

morphology of Purkinje cells 1.974694 5

cell spreading of tumor cell lines 1.970616 0.365 8

locomotion 1.970616 1.455 20

transcription of DNA 1.966576 0.381 74

hyperplasia of exocrine cells 1.966576 4

hydrolysis of nucleotide 1.958607 -0.314 9

hydrocephalus 1.958607 -1.387 7

liver cancer 1.946922 0.083 195

epileptic encephalopathy 1.943095 6

organization of intercellular junctions 1.943095 6

female genital neoplasm 1.931814 161

hereditary motor and sensory neuropathy 1.931814 7small GTPase mediated signal transduction 1.924453 Increased 2 12

polymerization of protein 1.924453 21

proliferation of connective tissue cells 1.920819 -0.19 32

congenital anomaly of genital system 1.920819 0.555 9

transport of vesicles 1.920819 9

activation of enzyme 1.917215 23

allergy 1.917215 1.218 23

development of granulocytes 1.917215 4

transformation of breast cell lines 1.917215 4

degradation of filaments 1.91364 3

immune thrombocytopenic purpura 1.91364 3

infectious lung disease 1.91364 3

laryngismus 1.91364 3

pectus excavatum 1.91364 3

pure red cell aplasia 1.91364 3

quantity of hepatocytes 1.91364 3

retinoblastoma cancer 1.91364 3

mature B-cell lymphoma 1.910095 12

size of neurons 1.906578 7

mating behavior 1.90309 5

cell death of breast cancer cell lines 1.899629 0.124 19

quantity of central nervous system cells 1.89279 1.129 10

hepatocellular carcinoma 1.88941 189

conditioning 1.886057 -0.726 13

cell movement of cancer cells 1.876148 -0.563 10

muscle contraction 1.872895 -1.108 15

colon adenocarcinoma 1.866461 157

cognitive impairment 1.860121 1 18

tumorigenesis of intestine 1.856985 169

thermal nociception 1.856985 -1 6

formation of membrane processes 1.847712 3

long-term potentiation of CA1 neuron 1.847712 3

development of gap junctions 1.844664 13abnormal morphology of marginal stria vascularis cells 1.844664 2

anxiety disorders with panic attacks 1.844664 2

atrophy of myotube 1.844664 2

endocochlear potential 1.844664 2

formation of clathrin-coated vesicles 1.844664 2

interphase of antigen presenting cells 1.844664 2

lack of otoliths 1.844664 2

metabolism of L-aspartic acid 1.844664 2

mild anemia 1.844664 2

myelination of optic nerve 1.844664 2

phagocytosis by Sertoli cells 1.844664 2phosphorylation of phosphatidylinositol-3-phosphate 1.844664 2

receptor-mediated endocytosis of vesicles 1.844664 2

repair of pyrimidine dimer 1.844664 2

temporal lobe epilepsy 1.844664 2

hypersensitive reaction 1.841638 1.111 24

anxiety 1.835647 0.933 12

bipolar I disorder 1.832683 5

function of neuroglia 1.832683 5

proliferation of cells 1.829738 1.451 164

abnormal morphology of interneurons 1.829738 4

clear cell ovarian cancer 1.829738 4

female genital tract cancer 1.829738 153

growth of connective tissue 1.826814 -0.326 34

cyanosis 1.826814 -0.156 9

homo-oligomerization of protein 1.826814 9

viability 1.812479 0.494 7abnormal morphology of rhombencephalon 1.806875 10

tumorigenesis of reproductive tract 1.8041 154

Table S9. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO hippocampus compared to WT controls. For each specified BioFunction/Disease annotation significantly populated the probability of enrichment (scored as –log10(p-value)) is stated along with the predicted activation state (Increased or Decreased), activation z-score (positive indicates activation and negative indicates inhibition) and number (#) of experimentally-derived input transcripts falling into the specific BioFunction/Disease pathway.

Diseases or Functions Annotation -log10(p-value) Activation State Activation z-score # Transcripts

organismal death 7.769551079 0.697 124

demyelinating peripheral neuropathy 5.749579998 10Movement Disorders 5.514278574 Decreased -2.213 61

morphology of cells 5.450996738 98

paralysis of limb 5.346787486 -1.254 7

Charcot-Marie-Tooth disease 5.242603971 8

organization of cytoplasm 5.127843727 0.39 79

neurological signs 4.675717545 0 40

quantity of carbohydrate 4.543633967 0.745 33

proliferation of cells 4.485452247 1.589 159

organization of cytoskeleton 4.467245621 0.39 71

paralysis of hindlimb 4.427128398 -0.97 6

abnormal morphology of cells 4.37161107 66

disorder of basal ganglia 4.229884705 44

organization of peroxisomes 4.228412519 5

abnormal morphology of nervous system 4.218244625 42

cell death of connective tissue cells 4.100726813 -0.495 36

Ras protein signal transduction 4.09420412 8

synthesis of GABA 4.077274542 4

organization of actin cytoskeleton 3.982966661 21

abnormal morphology of head 3.950781977 46

size of plasma membrane projections 3.910094889 4

amyloidosis 3.899629455 34

neuronal cell death 3.869666232 -0.798 39

dyskinesia 3.850780887 36

formation of focal adhesions 3.850780887 0.956 13

cell death 3.826813732 -0.239 143

quantity of cells 3.806875402 -1.435 84

pulmonary adenoma 3.782516056 0.418 6

formation of cellular protrusions 3.779891912 0.53 48

expression of RNA 3.744727495 -0.046 97

morphology of head 3.694648631 47

Dementia 3.661543506 33

morphology of nervous system 3.647817482 43

Alzheimer's disease 3.630784143 32

peroxisomal disorder 3.617982957 5

synthesis of protein 3.614393726 0.759 27

hereditary motor and sensory neuropathy 3.609064893 9

abnormal morphology of neurons 3.598599459 24

abnormal morphology of neurites 3.519993057 11

localization of protein 3.501689446 0.896 14

atrophy of muscle 3.425968732 12

quantity of mitochondria 3.403402904 -0.447 6

neuromuscular disease 3.390405591 46

Zellweger syndrome 3.375717904 4

proliferation of peroxisomes 3.375717904 4

size of head 3.373659633 0.415 15

apoptosis 3.297569464 -0.325 114Charcot-Marie-Tooth disease with deafness 3.26760624 2

G0/G1 phase transition of fibroblasts 3.26760624 2

Hutchinson-Gilford progeria syndrome 3.26760624 2

accumulation of skin cancer cell lines 3.26760624 2

development of endoderm progenitors 3.26760624 2

hyperplasia of mucus neck cell 3.26760624 2

mandibuloacral dysostosis 3.26760624 2

migration of cytotrophoblastic cells 3.26760624 2

phagocytosis of gonadal cell lines 3.26760624 2

shape of nucleus 3.26760624 2

depolarization of cellular membrane 3.26760624 8

peripheral neuropathy 3.234331445 12formation of plasma membrane projections 3.211831629 0.527 33

perinatal death 3.202040356 -0.24 33

internalization of fatty acid 3.19314197 3

migration of keratinocyte cancer cell lines 3.163675884 0.152 5

apoptosis of fibroblasts 3.148741651 -0.497 15

cell death of fibroblast cell lines 3.124938737 -0.478 25

transcription of RNA 3.116906641 0.181 83

transcription 3.108462542 -0.035 88

proliferation of neuronal cells 3.107905397 1.972 32

autosomal dominant myopathy 3.091514981 8

neuritogenesis 3.088842391 0.861 32

development of neurons 3.088842391 0.604 40

size of brain 3.077793723 0.365 12

cell death of fibroblasts 3.025028006 -0.421 17

adhesion of venule 3.024568191 3

migration of naive T lymphocytes 3.024568191 3

necrosis of epithelial tissue 3.003926346 0.692 32

morphology of neurons 3 25

quantity of male germ cells 2.995678626 -0.469 9

catabolism of fatty acid 2.982966661 4

concentration of D-glucose 2.966576245 1.217 20extension of plasma membrane projections 2.935542011 1.847 13small GTPase mediated signal transduction 2.935542011 1 13

abnormal morphology of axons 2.928117993 8

abnormal morphology of spermatids 2.924453039 7

abnormal morphology of interneurons 2.913640169 5

microtubule dynamics 2.903089987 0.84 56

development of head 2.903089987 -0.88 48abdominal cancer 2.89279003 Increased 2.345 326

abdominal neoplasm 2.89279003 1.558 329

autosomal recessive disease 2.88941029 42

synaptic transmission of nervous tissue 2.886056648 -0.447 9

abnormal morphology of right atrium 2.876148359 3

branching morphogenesis of lung 2.876148359 3

formation of peroxisomes 2.876148359 3

running 2.876148359 3

size of neurites 2.876148359 3

Huntington's Disease 2.863279433 31

formation of smooth muscle 2.860120914 5

digestive system cancer 2.850780887 1.408 285

morphogenesis of neurons 2.850780887 -0.206 24

Growth Failure 2.850780887 -0.402 32

pervasive developmental disorder 2.841637508 13

quantity of nervous tissue 2.838631998 0.596 24

morphology of cardiovascular system 2.818156412 35

Viral Infection 2.809668302 0.443 73

Charcot-Marie-Tooth disease type 2A 2.798602876 2arrest in cell cycle progression of hepatocytes 2.798602876 2

chemoattraction of thymocytes 2.798602876 2

co-localization of blood cells 2.798602876 2

concentration of lactic acid 2.798602876 2

development of medullary collecting duct 2.798602876 2

infection of peritoneal macrophages 2.798602876 2

lethal restrictive dermopathy 2.798602876 2lymphoepithelial sialadenitis of Sjogrens syndrome 2.798602876 2migration of chronic lymphocytic leukemia cells 2.798602876 2

progeroid form Ehlers-Danlos syndrome 2.798602876 2

redistribution of phosphatidylinositol 2.798602876 2

spontaneous fracture of rib 2.798602876 2

sporadic adenoma 2.798602876 2

contraction of striated muscle 2.785156152 -0.753 10

extension of cellular protrusions 2.777283529 1.469 14

digestive organ tumor 2.764471553 1.479 286

apoptosis of connective tissue cells 2.754487332 0.3 18

concentration of 22:6(n-3) fatty acids 2.747146969 3

internalization of lipid 2.744727495 4

sporadic tumor 2.744727495 4

progressive motor neuropathy 2.739928612 28

extension of neurites 2.732828272 1.631 12

Schizophrenia 2.730487056 28

morphology of heart 2.709965389 23

epithelial cancer 2.705533774 1.189 328

development of body axis 2.701146924 -0.88 50

metabolism of amino acid analogs 2.690369833 6

accumulation of vesicles 2.649751982 0.378 5

central nervous system tumor 2.643974143 0.553 42

import of lipid 2.628932138 3

morphogenesis of neurites 2.603800653 -0.206 23

myelination 2.595166283 -0.979 13

necrosis 2.590066877 -0.413 107

abnormal morphology of skull 2.583359493 14

extension of axons 2.581698709 1.097 8

mass of brain 2.576754126 0.128 6

demyelination 2.559090918 0.218 9

injury of heart 2.554395797 0.277 5

lymphadenopathy 2.554395797 -1.387 8abnormal morphology of reproductive system 2.552841969 27

quantity of neurons 2.552841969 0.794 23

expression of mRNA 2.55129368 0.152 15

autoimmune hemolytic anemia 2.540607512 4

proliferation of fibroblast cell lines 2.53165267 1.9 23

porphyria 2.522878745 3

degeneration of nervous system 2.517126416 0.198 17abnormal morphology of plasma membrane 2.515700161 12

neuroepithelial tumor 2.511449283 -0.321 37

borderline tumor 2.503070352 2

breakdown of fatty acid 2.503070352 2

carcinoma of ampulla of Vater 2.503070352 2

demyelination of sciatic nerve 2.503070352 2

development of plasma cells 2.503070352 2

import of fatty acid 2.503070352 2

juvenile gastrointestinal polyposis 2.503070352 2

morphology of delta islet cells 2.503070352 2

polarity of T lymphocytes 2.503070352 2

recruitment of M2 macrophages 2.503070352 2

shape change of nucleus 2.503070352 2

muscle contraction 2.493494968 -0.377 15

synthesis of heme 2.480172006 4

abnormal morphology of brain 2.477555766 24

metabolism of amino acids 2.474955193 1.154 11

glandular intraepithelial neoplasm 2.468521083 9

DNA damage checkpoint 2.46470588 5

metabolism of porphyrin 2.46470588 5

migration of cells 2.447331784 0.172 81

glioma cancer 2.437707136 15autosomal dominant Charcot-Marie-Tooth disease type 2 2.424812155 3

cell movement of leukemia cells 2.424812155 3

metastasis of lymph node 2.420216403 4

tumorigenesis of tissue 2.412289035 0.857 333

urothelial neoplasm 2.401209493 6

neurodegeneration of axons 2.381951903 -0.555 7

differentiation of gonadal cells 2.380906669 5

function of Th1 cells 2.380906669 5

concentration of lipid 2.378823718 -0.727 39

lymph node cancer 2.367542708 6

infertility 2.36251027 19

uterine serous papillary cancer 2.360513511 14

gliomatosis 2.339134522 -0.321 36

development of cytoplasm 2.336299075 -1.51 24

Rasmussen's encephalitis 2.335358024 3

chemotaxis of brain cells 2.335358024 3

chemotaxis of neurons 2.335358024 3

differentiation of male germ cells 2.335358024 3

migration of trophoblast cells 2.335358024 3

morphology of lung cancer cell lines 2.335358024 3

release of blood cells 2.335358024 3

cell polarity formation 2.335358024 6

catabolism of amino acids 2.303643611 6

quantity of epithelial cells 2.298432015 -0.194 9

metabolism of carbohydrate 2.297569464 0.531 31abnormal morphology of zymogen granules 2.288192771 2

conversion of placental cells 2.288192771 2

cross-linkage of protein 2.288192771 2

degeneration of optic nerve 2.288192771 2

endoreduplication of DNA 2.288192771 2

homing of cancer cells 2.288192771 2

left pulmonary isomerism 2.288192771 2

localization of fatty acid 2.288192771 2metabolism of UDP-N-acetyl-D-galactosamine 2.288192771 2

metabolism of protoporphyrinogen 2.288192771 2

organization of myofilaments 2.288192771 2

ovarian cystadenocarcinoma 2.288192771 2

passive systemic anaphylaxis 2.288192771 2

release of hematopoietic progenitor cells 2.288192771 2

size of axons 2.288192771 2

survival of mesangial cells 2.288192771 2

Sjogren's syndrome 2.262807357 8

morphology of cellular protrusions 2.254144805 12

spermiation 2.253365801 3

transitional cell bladder cancer 2.253365801 3

laminopathy 2.241845378 6

abnormal morphology of male germ cells 2.240332155 8

gliosis 2.238072162 -1.605 9

function of T lymphocytes 2.227678293 15

cell death of embryonic cell lines 2.223298816 0.172 14

seizure disorder 2.218963061 0.537 24

lipodystrophy 2.207608311 4

size of body 2.205511953 -1.211 40

translation 2.202040356 0.656 15

endoplasmic reticulum stress response 2.179142011 0.492 9

development of central nervous system 2.179142011 -0.889 31

cell spreading of cervical cancer cell lines 2.176525771 3chronic inflammatory demyelinating polyradiculoneuropathy 2.176525771 3

condensation of cells 2.176525771 3

initiation of chemoattraction of cells 2.176525771 3

metabolism of neutral amino acid 2.176525771 3

incidence of benign tumor 2.17327748 1.212 7

kyphosis 2.17327748 -1.026 7

cell movement of hematopoietic cell lines 2.17327748 -1.057 7

Neurodegeneration 2.170696227 0.212 17

quantity of gonadal cells 2.166852888 -0.429 11

growth of axons 2.164943898 1.131 10

esterification of lipid 2.151810883 -0.849 5polarity of cells 2.151810883 Decreased -2 5

motor dysfunction 2.148741651 -1.949 7

secretion of neurotransmitter 2.148130399 -1.206 10

mass of organism 2.147520006 -1.198 18

abnormal morphology of cerebellum 2.141462802 9

cell death of epithelial cells 2.140861703 0.803 25

damage of heart 2.125518182 0.64 6accumulation of phosphatidylinositol 4,5-diphosphate 2.118615343 2

adhesion of naive T lymphocytes 2.118615343 2

chemotaxis of naive T lymphocytes 2.118615343 2colony formation of kidney cancer cell lines 2.118615343 2

conversion of chondrocytes 2.118615343 2

decarboxylation of alpha-amino acid 2.118615343 2

formation of GW body 2.118615343 2

hepatic porphyria 2.118615343 2

hydrolysis of 2-arachidonoylglycerol 2.118615343 2

hypertelorism 2.118615343 2

metabolism of vitamin K 2.118615343 2

onset of motor neuron disease 2.118615343 2

priming of Th2 cells 2.118615343 2

quantity of GW body 2.118615343 2

release of blood platelets 2.118615343 2

repair of gene 2.118615343 2transepithelial electrical resistance of bronchial epithelial cells 2.118615343 2

cell cycle progression of epithelial cells 2.112945622 4

expansion of stem cells 2.112945622 4

central nervous system cancer 2.111820506 36abnormal morphology of cerebral neocortex 2.104577454 3

abnormal skeletal muscle fiber type ratio 2.104577454 3

immune thrombocytopenic purpura 2.104577454 3

infectious lung disease 2.104577454 3

proliferation of mesothelioma cell lines 2.104577454 3

pure red cell aplasia 2.104577454 3

catecholamine-secreting tumor 2.104025268 9

dysplasia of skeleton 2.104025268 9

experimentally-induced diabetes 2.101274818 7

tumorigenesis of benign tumor 2.101274818 -0.262 7abnormal morphology of cardiovascular system 2.090979146 30

development of exocrine gland 2.089375595 13

apoptosis of embryonic cell lines 2.088842391 -0.057 11

synaptic transmission of synapse 2.083019953 5

infection by RNA virus 2.078313525 1.523 40

morphogenesis of dendrites 2.077793723 7congenital anomaly of cardiovascular system 2.076238039 0.84 13

autosomal dominant disease 2.075204004 29

prostatic intraepithelial neoplasia 2.07109231 8

respiratory failure 2.07109231 -1.488 8

synaptic transmission of cells 2.069560405 -0.728 14

tumorigenesis of papilloma 2.068033885 4

endocytosis 2.064996849 1.157 18synthesis of reactive oxygen species 2.057495894 Increased 2.105 24congenital anomaly of musculoskeletal system 2.047207557 -0.081 34

function of lymphocytes 2.044793462 18

infection of cells 2.038578906 1.811 37

chemorepulsion of cells 2.037157319 3

formation of renal tubule 2.037157319 3

hyperbilirubinemia 2.037157319 3

hypotrichosis 2.037157319 3

size of corpus callosum 2.037157319 3

quantity of glycogen 2.032452024 -1.254 7

quantity of amino acids 2.028724151 1.176 11

formation of cytoplasmic aggregates 2.025028006 4

heart conduction disorder 2.025028006 4

neurotransmission 2.018181393 -0.342 21

urothelial cancer 2.017276612 5

injury of cells 2.017276612 -0.03 6

paralysis 2.015472687 -1.58 10

replication of RNA virus 2.012333735 -1.827 25

colony formation of cells 2.008773924 0.684 24cellular homeostasis 1.995678626 Decreased -2.03 64

esterification of cholesterol 1.982966661 4

congenital heart disease 1.982966661 1.226 12

size of lymph follicle 1.982966661 -1.067 4

adhesion of postcapillary venule 1.978810701 2

anoikis of lung cancer cell lines 1.978810701 2

appearance of hair 1.978810701 2

branching of fibroblast cell lines 1.978810701 2

expansion of endothelial cells 1.978810701 2

formation of clathrin-coated vesicles 1.978810701 2

infiltration by plasma cells 1.978810701 2

localization of antibody 1.978810701 2

metastasis of adenocarcinoma 1.978810701 2

proliferation of uterine cell lines 1.978810701 2

Ehlers-Danlos syndrome 1.974694135 3

Stevens-Johnson syndrome 1.974694135 3

common variable immunodeficiency 1.974694135 3

concentration of GABA 1.974694135 3

conversion of connective tissue cells 1.974694135 3

malocclusion 1.974694135 3

situs inversus 1.974694135 3

sporadic motor neuron disease 1.974694135 3

embryonal tumor 1.974694135 13

neonatal death 1.974694135 0.789 22

neuroblastoma 1.966576245 7

protein kinase cascade 1.958607315 -0.616 21

cell death of kidney cells 1.954677021 0.021 18differentiation of osteoblasts 1.954677021 Decreased -2.234 15

formation of cytoskeleton 1.946921557 -1.684 19

differentiation of cells 1.946921557 -1.739 91

accumulation of phosphoinositide 1.943095149 4

benign neoplasm of liver 1.943095149 4

morphogenesis of lung 1.943095149 4

growth of neurites 1.943095149 1.293 23

gastrointestinal carcinoma 1.935542011 175

female genital tract serous cancer 1.928117993 17

dysgenesis 1.928117993 0.455 26

squamous cell skin cancer 1.924453039 5

quantity of germ cells 1.920818754 -0.745 10

abnormal morphology of sperm midpiece 1.913640169 3

celiac disease 1.913640169 3

deposition of Ca2+ 1.913640169 3

injury of epithelial tissue 1.913640169 3

morphology of carcinoma cell lines 1.913640169 3

transmission of lipid 1.913640169 3

translation of protein 1.913640169 0.994 14

B-cell neoplasm 1.910094889 0.832 19

cell movement 1.910094889 0.032 86

abnormal morphology of mitochondria 1.903089987 4HIV infection 1.899629455 Increased 2.068 33

hydrolysis of nucleotide 1.899629455 -0.863 8reorganization of cytoskeleton 1.899629455 Decreased -2.588 11autosomal dominant Emery-Dreifuss muscular dystrophy 1.896196279 5

seizures 1.896196279 0.537 20

abnormal morphology of cerebellar cortex 1.89279003 6

activation of microglia 1.882728704 0 7

branching of neurons 1.879426069 -0.25 15

transformation of fibroblasts 1.879426069 -0.562 8

dendritic growth/branching 1.876148359 -0.792 12

arrest in cell cycle progression 1.869666232 17

abnormal morphology of testis 1.869666232 14

Hypertrophy 1.869666232 -0.211 23

function of helper T lymphocytes 1.866461092 6

abnormal morphology of skull vault 1.866461092 4

intestinal polyp 1.866461092 4abnormal morphology of anterior segment of the eye 1.860120914 7

formation of hair follicle 1.860120914 7

apoptosis of dermal fibroblasts 1.860120914 2

catabolism of acidic amino acid 1.860120914 2

chemorepulsion of brain cells 1.860120914 2

chemorepulsion of neurons 1.860120914 2

cytokinesis of fibroblast cell lines 1.860120914 2

development of Purkinje cells 1.860120914 2

development of naive T lymphocytes 1.860120914 2

differentiation of duct cells 1.860120914 2

eyelids fail to open 1.860120914 2

hereditary hypotrichosis 1.860120914 2

hyperplasia of retina 1.860120914 2

injury of epithelial cells 1.860120914 2onset of experimental autoimmune encephalomyelitis 1.860120914 2

refractory acute myeloid leukemia 1.860120914 2

respiration of fibroblast cell lines 1.860120914 2

size of bone cancer cell lines 1.860120914 2

size of skin tumor 1.860120914 2

uptake of eicosanoid 1.860120914 2

expression of protein 1.860120914 1.523 17

endocytosis of protein 1.8569852 3

lipofuscinosis 1.8569852 3

paroxysmal movement disorder 1.8569852 3

type M3 acute myeloid leukemia 1.8569852 3

glioblastoma cancer 1.850780887 13

mass of gonad 1.850780887 1.29 12

dystrophy of muscle 1.850780887 -0.784 12

morphology of cytoskeleton 1.841637508 7

outgrowth of axons 1.841637508 7

tumorigenesis of adenoma 1.838631998 0.456 5

synthesis of carbohydrate 1.838631998 0.313 21

apoptosis of oligodendrocytes 1.829738285 4

concentration of cyclic GMP 1.829738285 -0.812 4

reorganization of actin cytoskeleton 1.826813732 8

Gastrointestinal Tract Cancer and Tumors 1.818156412 1.268 194

intestinal polyposis 1.804100348 3

metabolism of amino sugar 1.804100348 3

morphology of lens tissue 1.804100348 3

proliferation of kidney cell lines 1.804100348 -0.57 10

colony formation 1.801342913 0.508 25

cell movement of brain cells 1.801342913 -1.673 7

organization of organelle 1.795880017 25

hydrolysis of acylglycerol 1.793174124 1.122 4

activation of neuroglia 1.793174124 0 8

amyotrophic lateral sclerosis 1.790484985 12

gastrointestinal tract cancer 1.790484985 1.109 192

outgrowth of neurites 1.787812396 0.911 20

Hypoplasia 1.785156152 0.634 24

synaptic transmission 1.785156152 -0.342 17

incidence of adenoma 1.782516056 1.35 5

abnormal morphology of genital organ 1.779891912 20

metabolism of protein 1.779891912 1.467 42

abnormal morphology of heart 1.777283529 19

congenital anomaly of skin 1.777283529 10

cell death of brain 1.764471553 -1.013 15

abnormal morphology of bent tail 1.759450752 4

demyelination of nerves 1.759450752 4

activation of sympathetic nerve 1.759450752 2

acute immune thrombocytopenic purpura 1.759450752 2

apoptosis of germ cell tumor cell lines 1.759450752 2

arousal 1.759450752 2arrest in cell cycle progression of muscle cells 1.759450752 2

catabolism of branched chain amino acids 1.759450752 2

chronic brain damage 1.759450752 2chronic immune thrombocytopenic purpura 1.759450752 2

concentration of docosahexaenoic acid 1.759450752 2

concentration of phosphocreatine 1.759450752 2

congenital agammaglobulinemia 1.759450752 2

discriminatory learning 1.759450752 2

extended lifespan 1.759450752 2

familial combined hyperlipidemia 1.759450752 2

familial partial lipodystrophy 1.759450752 2

fetal erythroblastosis 1.759450752 2

formation of external granular layer 1.759450752 2

formation of granule cells 1.759450752 2

infection of fibroblasts 1.759450752 2

meteorism 1.759450752 2

pancreatic cyst 1.759450752 2

production of histamine 1.759450752 2

recurrent sinopulmonary infection 1.759450752 2

redox signal response 1.759450752 2

replication of Sendai virus 1.759450752 2

sporadic psychological disorder 1.759450752 2

swelling of axons 1.759450752 2

transformation of tumor cells 1.759450752 2

transmission of sterol 1.759450752 2

transport of sugar acid 1.759450752 2

incidence of carcinoma 1.759450752 0.941 8

quantity of dendritic spines 1.759450752 -0.187 4

actinic keratosis 1.754487332 3

cell movement of thymocytes 1.754487332 3

light chain associated amyloidosis 1.754487332 3

Substance-Related Disorders 1.754487332 9

skin abnormality 1.754487332 12cell movement of cervical cancer cell lines 1.754487332 0.026 6differentiation of erythroid progenitor cells 1.754487332 -0.562 6

astrocytoma 1.749579998 28

apoptosis of neurons 1.749579998 -1.493 21

G2/M phase transition 1.744727495 7

transport of carboxylic acid 1.744727495 1.912 7

activation of antigen presenting cells 1.744727495 -0.407 16

organization of cellular membrane 1.742321425 8

outgrowth of cells 1.742321425 0.714 21

abnormal morphology of gonad 1.735182177 18

formation of eye 1.735182177 21

import of D-glucose 1.730487056 -0.152 5

morphology of body cavity 1.728158393 51

size of tumor cell lines 1.725842151 4

release of histamine 1.725842151 0 4

myoclonus 1.725842151 -1.067 4

translation of mRNA 1.721246399 0.537 10

Parkinson's disease 1.716698771 13

size of neurons 1.71219827 6

apoptosis of dermal cells 1.71219827 0.391 6

development of malignant tumor 1.709965389 9

development of sensory organ 1.709965389 26infection by HIV-1 1.709965389 Increased 2.247 28

aphagia 1.705533774 3proliferation of rhabdomyosarcoma cell lines 1.705533774 3

Table S10. Ingenuity-based BioFunction/Disease analysis for analysis for transcripts differentially regulated in T1R3KO hypothalamus compared to WT controls. For each specified BioFunction/Disease annotation significantly populated the probability of enrichment (scored as –

log10(p-value)) is stated along with the predicted activation state (Increased or Decreased), activation z-score (positive indicates activation and negative indicates inhibition) and number (#) of experimentally-derived input transcripts falling into the specific BioFunction/Disease pathway.

Diseases or Functions Annotation -log10(-pvalue) Activation State Activation z-score # Transcripts

morphology of cells 6.90E+00 84

gliosis 5.84E+00 -0.404 13

demyelination of nerves 5.01E+00 1.912 7

Cytosis 4.24E+00 -0.973 19

hepatic porphyria 3.97E+00 3

abnormal morphology of cells 3.92E+00 52

gliosis of hippocampus 3.82E+00 4

chronic sinusitis 3.74E+00 3

myelination 3.67E+00 -1.157 13

quantity of macrophages 3.67E+00 -0.883 13

cell death 3.60E+00 0.912 112

morphology of neurons 3.52E+00 22

organization of peroxisomes 3.51E+00 4

G0/G1 phase transition of fibroblasts 3.50E+00 2

cecum adenoma 3.50E+00 2

porphyria variegata 3.50E+00 2

pseudomyxoma peritonei 3.50E+00 2

pyloric-like gland adenoma 3.50E+00 2

sporadic colorectal carcinoma 3.50E+00 2

mucinous neoplasm 3.44E+00 5

infiltration 3.44E+00 0.618 22

demyelination 3.38E+00 1.349 9

sinusitis 3.34E+00 4

gliosis of central nervous system 3.34E+00 -0.152 6

quantity of cells 3.25E+00 -0.671 65

necrosis 3.24E+00 0.265 88

formation of peroxisomes 3.22E+00 3

permeabilization of liposome 3.22E+00 3

astrocytosis 3.20E+00 -0.092 7

lack of ovarian follicle 3.17E+00 5

female genital tract serous cancer 3.11E+00 17

glioma cancer 3.11E+00 14

Schizophrenia 3.08E+00 24

astrocytosis of hippocampus 3.08E+00 3

cell movement 3.04E+00 0.729 74

peroxisomal disorder 3.04E+00 4

adenocarcinoma of the appendix 3.03E+00 2

apoptosis of activated T lymphocyte 3.03E+00 2communication of nervous tissue cell lines 3.03E+00 2

diverticular disease 3.03E+00 2

infection of peritoneal macrophages 3.03E+00 2

intraductal papillary biliary neoplasm 3.03E+00 2

myxoma 3.03E+00 2

pancreatic intraductal papillary mucinous tumor 3.03E+00 2

whisker trimming 3.03E+00 2

infiltration by mononuclear leukocytes 3.01E+00 0.244 11

size of lesion 3.01E+00 -0.792 14

organization of cytoplasm 3.01E+00 0.806 56

myelination of cells 3.01E+00 8

cell death of epithelial cell lines 2.99E+00 -0.144 15

proliferation of cells 2.99E+00 1.654 118

fusion of endosomes 2.97E+00 4

central nervous system tumor 2.93E+00 0.152 35

cell movement of tumor cell lines 2.92E+00 0.687 34

endocytosis 2.90E+00 1.382 17

activation of leukocyte cell lines 2.89E+00 1 5

abnormal morphology of inner hair cells 2.86E+00 3

colorectal polyposis 2.86E+00 3

toxic epidermal necrolysis 2.86E+00 3

neuronal cell death 2.86E+00 0.279 29

glioblastoma cancer 2.82E+00 13

adhesion of kidney cell lines 2.76E+00 0.152 5

morphology of Purkinje cells 2.76E+00 5

transport of molecule 2.76E+00 1.129 56

atherosclerosis of aortic root 2.76E+00 3

lack of CD8+ T lymphocyte 2.76E+00 3

area of neointima 2.74E+00 2

communication of endothelial cell lines 2.74E+00 2

duodenal adenoma 2.74E+00 2

hyperplasia of alpha islet cells 2.74E+00 2idiopathic sensorineural hearing impairment 2.74E+00 2

import of fatty acid 2.74E+00 2lipopolysaccharide hepatocellular cholestasis 2.74E+00 2morphology of keratinocyte cancer cell lines 2.74E+00 2cell movement of mononuclear leukocytes 2.73E+00 0.567 21

infiltration of cells 2.73E+00 0.618 19

plasticity of synapse 2.71E+00 8

mucinous adenocarcinoma 2.68E+00 4

morphology of tumor cell lines 2.66E+00 11

apoptosis of epithelial cell lines 2.65E+00 0.552 12

infiltration of leukocytes 2.64E+00 0.515 18

migration of cells 2.59E+00 0.753 65

Zellweger syndrome 2.58E+00 3

binding of kidney cell lines 2.58E+00 0.6 5

binding of tumor cell lines 2.53E+00 -0.23 10

delay in myelination 2.52E+00 2

duodenal adenocarcinoma 2.52E+00 2metabolism of UDP-N-acetyl-D-galactosamine 2.52E+00 2

organization of myofilaments 2.52E+00 2

quantity of neuroblastoma cell lines 2.52E+00 2

quantity of renal lesion 2.52E+00 2

rectal adenoma 2.52E+00 2

transport of oxygen 2.52E+00 2

differentiation of cells 2.51E+00 -0.349 75

cystitis 2.50E+00 3

activation of microglia 2.50E+00 -0.112 7

benign neoplasm of female genital organ 2.49E+00 17

cell death of fibroblast cell lines 2.49E+00 -0.466 19

clear cell ovarian cancer 2.48E+00 4

activation of neuroglia 2.47E+00 0.213 8

localization of protein 2.44E+00 0.339 10

cholangiocarcinoma 2.44E+00 5

refolding of protein 2.43E+00 3

morphology of nervous system 2.42E+00 31small GTPase mediated signal transduction 2.40E+00 10

Encephalitis 2.40E+00 0.249 14

neurocutaneous syndrome 2.39E+00 4

abnormal morphology of neurons 2.38E+00 17

endoplasmic reticulum stress response 2.37E+00 8

adhesion of kidney cells 2.37E+00 0.651 6

central nervous system cancer 2.37E+00 30organismal death 2.36E+00 Increased 2.523 78

aggregation of mitochondria 2.36E+00 3

cytotoxicity of cytotoxic T cells 2.35E+00 -0.651 4

breakage of Plasma Membrane 2.35E+00 2

cell-cell adhesion of kidney cell lines 2.35E+00 2

cleavage of acyl-coenzyme A 2.35E+00 2

excursion of D-glucose 2.35E+00 2

gastric adenoma 2.35E+00 2

hypomyelination of axons 2.35E+00 2

obstructive cholestasis 2.35E+00 2

quantity of gastric parietal cells 2.35E+00 2

adenoma 2.34E+00 19

metabolism of protein 2.33E+00 0.937 36

quantity of metal 2.32E+00 0.835 21

gliomatosis 2.30E+00 29

benign ovarian tumor 2.30E+00 8

Stevens-Johnson syndrome 2.30E+00 3

apoptosis of peritoneal macrophages 2.30E+00 3

hypogammaglobulinemia 2.30E+00 3

plasticity of neuronal synapse 2.30E+00 3

cell movement of monocytes 2.27E+00 0.246 9

gastrointestinal stromal tumor 2.27E+00 4

apoptosis of kidney 2.25E+00 1.195 7

behavior 2.25E+00 0.658 37

Kawasaki's disease 2.23E+00 4

size of islets of Langerhans 2.23E+00 4

insulin-dependent diabetes mellitus 2.23E+00 19

abnormal morphology of ovarian follicle 2.23E+00 6

migration of blood cells 2.22E+00 0.907 33

abnormal morphology of superficial fascia 2.21E+00 2

branching of fibroblast cell lines 2.21E+00 2

colon tubulovillous adenoma 2.21E+00 2

differentiation of hippocampal neurons 2.21E+00 2

inhibition of natural killer cells 2.21E+00 2

pancreatic adenoma 2.21E+00 2

resealing of plasma membrane 2.21E+00 2

quantity of phagocytes 2.21E+00 -1.688 18

Wound 2.19E+00 11

cognition 2.18E+00 0.152 19

endocytosis of protein 2.18E+00 3

growth of lung carcinoma 2.18E+00 3

Duchenne muscular dystrophy 2.17E+00 7

receptor-mediated endocytosis 2.17E+00 8

binding of cAMP response element 2.12E+00 3

differentiation of breast cell lines 2.12E+00 3

hematopoiesis of spleen 2.12E+00 3

infection of macrophages 2.12E+00 3

mucinous ovarian cancer 2.12E+00 3

sporadic tumor 2.12E+00 3

atherosclerosis of aorta 2.12E+00 4

benign neoplasia 2.11E+00 30

infertility 2.10E+00 15

cell movement of leukocytes 2.10E+00 0.777 29

experimentally-induced diabetes 2.09E+00 6

invasion of lung cancer cell lines 2.09E+00 -0.7 6

adenomatous goiter 2.09E+00 2

binding of lung cancer cell lines 2.09E+00 2childhood T acute lymphoblastic leukemia 2.09E+00 2

formation of multivesicular bodies 2.09E+00 2

fusion of late endosomes 2.09E+00 2onset of experimental autoimmune encephalomyelitis 2.09E+00 2proliferation of non-small-cell lung cancer cells 2.09E+00 2

transformation of intestinal cell lines 2.09E+00 2

translocation of cholesterol 2.09E+00 2

paired-pulse facilitation of synapse 2.09E+00 0.277 4

Movement Disorders 2.08E+00 1.203 38

systemic autoimmune syndrome 2.08E+00 0.14 38

colony formation of leukemia cell lines 2.07E+00 3

modulation of cells 2.07E+00 3

growth of dendrites 2.07E+00 0.492 5

hearing loss 2.06E+00 1.195 10

abnormal morphology of Purkinje cells 2.05E+00 4

migration of tumor cell lines 2.05E+00 1.017 26

intrahepatic cholestasis 2.04E+00 5

cellular homeostasis 2.04E+00 1.809 51

abnormal morphology of ovary 2.03E+00 7

growth of neurites 2.02E+00 0.45 19

fibrin clot 2.02E+00 3

fusion of lysosome 2.02E+00 3

internalization of protein 2.01E+00 6

translation 2.01E+00 -1.016 12

apoptosis 2.01E+00 0.108 83

function of heart 2.00E+00 -1.912 7

infiltration by lymphocytes 2.00E+00 0.216 8

leukocyte migration 2.00E+00 1.059 32abnormal morphology of CD8-positive alpha-beta cytotoxic T cells 1.98E+00 2acute immune thrombocytopenic purpura 1.98E+00 2

autophagy of cancer cells 1.98E+00 2catabolism of branched chain amino acids 1.98E+00 2chronic immune thrombocytopenic purpura 1.98E+00 2

colonic polyposis 1.98E+00 2

congenital agammaglobulinemia 1.98E+00 2development of alpha-beta T lymphocytes 1.98E+00 2

discriminatory learning 1.98E+00 2

fetal erythroblastosis 1.98E+00 2

hemochromatosis 1.98E+00 2

infection of fibroblasts 1.98E+00 2

phagocytosis of kidney cell lines 1.98E+00 2

production of histamine 1.98E+00 2

proliferation of adipoblasts 1.98E+00 2

recurrent sinopulmonary infection 1.98E+00 2

peripheral neuropathy 1.98E+00 8

colony formation of tumor cell lines 1.98E+00 0.984 12

X-linked hereditary disease 1.97E+00 13

function of cardiovascular system 1.97E+00 -1.912 13

activation of cells 1.97E+00 1.363 35

apoptosis of bladder cancer cell lines 1.97E+00 3

loss of axons 1.97E+00 3

proliferation of liver cell lines 1.97E+00 3

scar tissue 1.97E+00 3

rheumatoid arthritis 1.96E+00 26

homeostasis of metal ion 1.96E+00 9

invasion of carcinoma cell lines 1.95E+00 -0.436 7

accumulation of leukocytes 1.94E+00 0.057 12

cell death of embryonic cell lines 1.94E+00 -0.419 11

morphogenesis of neurites 1.94E+00 -0.93 17

learning 1.93E+00 17

familial brain tumor syndrome 1.93E+00 3

idiopathic dilated cardiomyopathy 1.93E+00 3

iron overload 1.93E+00 3

movement of rodents 1.93E+00 10

cell death of connective tissue cells 1.91E+00 -0.958 23

damage of kidney 1.90E+00 0.98 7glucose metabolism disorder 1.90E+00 Decreased -2.008 44

cytotoxicity of lymphocytes 1.90E+00 -0.863 8

binding of cells 1.90E+00 -0.248 19

X-linked agammaglobulinemia 1.89E+00 2

accumulation of anion 1.89E+00 2arrest in interphase of hepatoma cell lines 1.89E+00 2

atrophy of ovary 1.89E+00 2

cell cycle progression of myeloid cells 1.89E+00 2

cervical adenocarcinoma 1.89E+00 2

chronic interstitial cystitis 1.89E+00 2

exocytosis of dense-core vesicles 1.89E+00 2

formation of sarcomere 1.89E+00 2newly diagnosed Ph positive chronic myeloid leukemia in chronic phase 1.89E+00 2

transport of L-proline 1.89E+00 2

tuberous sclerosis 1.89E+00 2abnormal morphology of placental spongiotrophoblast layer 1.88E+00 3apoptosis of macrophage cancer cell lines 1.88E+00 3

microcytic anemia 1.88E+00 3

thrombus 1.88E+00 5

apoptosis of renal tubule 1.87E+00 0 4

function of blood vessel 1.87E+00 4

astrocytoma 1.86E+00 23development of neurons 1.85E+00 Decreased -2.382 28

cell movement of phagocytes 1.85E+00 -0.052 21

adhesion of eosinophils 1.84E+00 3

differentiation of epithelial cell lines 1.84E+00 3

inborn error of metal metabolism 1.84E+00 3

myelination of nerves 1.84E+00 3

serous ovarian carcinoma 1.84E+00 7

nonischemic cardiomyopathy 1.84E+00 10

disorder of basal ganglia 1.84E+00 28secretion of protein 1.82E+00 Increased 2.236 8

proliferation of neuronal cells 1.82E+00 0.272 22

apoptosis of islet cells 1.82E+00 1 5

apoptosis of gonadal cell lines 1.81E+00 4

morphology of kidney cells 1.81E+00 4

polyneuropathy 1.81E+00 6

acidemia 1.81E+00 2

congenital heart block 1.81E+00 2

conversion of fibroblasts 1.81E+00 2

cystadenoma 1.81E+00 2growth of Candida albicans ATCC 90028 1.81E+00 2

guanine nucleotide exchange 1.81E+00 2

infarction of heart 1.81E+00 2

neonatal alloimmune thrombocytopenia 1.81E+00 2

paternal imprinting 1.81E+00 2

transport of essential amino acids 1.81E+00 2

whooping cough 1.81E+00 2

branching of cells 1.81E+00 -0.846 15

myelination of neurons 1.80E+00 3

diabetes mellitus 1.80E+00 37abnormal morphology of cerebellar cortex 1.79E+00 5

invasion of cells 1.79E+00 0.706 30

dilation of heart chamber 1.79E+00 1 4

myelination of nervous system 1.77E+00 5

organization of cytoskeleton 1.77E+00 0.806 46

urological disorder of kidney 1.76E+00 4

urothelial cancer 1.76E+00 4

neurological signs 1.75E+00 24

Rho protein signal transduction 1.75E+00 5Table S11. Proteins altered in expression between T1R3KO and WT hippocampal tissues. For each protein reliabily identified as being differentially regulated between T1R3KO and WT hippocampus the protein accession, Gene Symbol, Description and the log2 transform of the T1R3KO:WT iTRAQ ratio is displayed. Proteins demonstrating a difference of expression outside of the 95% confidence limits are highlighted in red (upregulated in T1R3KO compared to WT) or green (downregulated in T1R3KO compared to WT).

Protein Accession

Gene Symbol Protein Description Log2 iTRAQ ratio

81916660Cadm3

Cell adhesion molecule 31.060526336

18203574Uchl5

Ubiquitin carboxyl-terminal hydrolase isozyme L51.051728731

2829482Slc8a1

Sodium/calcium exchanger 11.039097993

123788967Fam92b

Protein FAM92B1.01090024

47117306Rps19

40S ribosomal protein S190.987158528

51315949Fbxo2

F-box only protein 20.900195816

20139259Pin1

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 10.832141687

54038801Ppp2ca

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform0.818459445

90110410Mpst

3-mercaptopyruvate sulfurtransferase0.801215378

21263374Acly

ATP-citrate synthase0.795662875

341942150Rpl3

60S ribosomal protein L30.789571332

56404602Ablim2

Actin-binding LIM protein 20.778720389

62511116Rap2a

Ras-related protein Rap-2a0.776670565

50400458Cnksr2

Connector enhancer of kinase suppressor of ras 20.771420348

77416862Tubgcp3

Gamma-tubulin complex component 30.71827828

34098594Eppk1

Epiplakin0.703465122

25453324Vps29

Vacuolar protein sorting-associated protein 290.649988186

81878370Arhgap18

Rho GTPase-activating protein 180.642050743

47117239Scamp1

Secretory carrier-associated membrane protein 10.638303729

85681904Uso1

General vesicular transport factor p1150.636255459

549061Cct6a

T-complex protein 1 subunit zeta0.607832677

55976513Eif4b

Eukaryotic translation initiation factor 4B0.598571905

2499837Psmb1

Proteasome subunit beta type-10.592903473

462064Fabp5

Fatty acid-binding protein, epidermal0.587444575

20522240Tpm1

Tropomyosin alpha-1 chain0.558142738

5915871Calu

Calumenin0.555574557

46395932Gprc5b

G-protein coupled receptor family C group 5 member B0.544592639

341942152Rimbp2

RIMS-binding protein 20.540528654

172044688Ehd3

EH domain-containing protein 30.534631122

2829840Atp5j

ATP synthase-coupling factor 6, mitochondrial0.534474025

24211558Clic4

Chloride intracellular channel protein 40.52942874

118573056Smek2

Serine/threonine-protein phosphatase 4 regulatory subunit 3B0.522844133

147644461Gpd1l

Glycerol-3-phosphate dehydrogenase 1-like protein0.51301227

55976533Matr3

Matrin-30.508543619

62286597Diras2

GTP-binding protein Di-Ras20.507628505

29840839Pebp1

Phosphatidylethanolamine-binding protein 10.502746802

166223488Stox2

Storkhead-box protein 20.495488902

123788828Olfm4

Olfactomedin-40.491471891

41688568Slc9a3r1

Na(+)/H(+) exchange regulatory cofactor NHE-RF10.48625935

52783597Gng3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-30.473430716

32363497Ezr

Ezrin0.468320791

341942049Snx6

Sorting nexin-60.466591416

9910790Tagln3

Transgelin-30.459376954

1350914Rxrg

Retinoic acid receptor RXR-gamma0.44643791

45477305Sept8

Septin-80.440794921

52782768D10Jhu81e

ES1 protein homolog, mitochondrial0.439949367

81906255Aif1l

Allograft inflammatory factor 1-like0.436491441

51701335Ctdsp2

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 20.433191734

116849Cfl1

Cofilin-10.432117719

51702253Cplx1

Complexin-10.430221883

77416861Gak

Cyclin-G-associated kinase0.429597367

15214055Eif4h

Eukaryotic translation initiation factor 4H0.424378219

37077864Auh

Methylglutaconyl-CoA hydratase, mitochondrial0.422168819

257051017Hagh

Hydroxyacylglutathione hydrolase, mitochondrial0.420851606

122065897Plec1

Plectin0.418621639

81879780Sncb

Beta-synuclein0.413574556

146345428Gpd2

Glycerol-3-phosphate dehydrogenase, mitochondrial0.410231249

341941034Kif5c

Kinesin heavy chain isoform 5C0.409371666

81896015Samm50

Sorting and assembly machinery component 50 homolog0.407396487

8134322 Atp8a1 Probable phospholipid-transporting ATPase IA0.396925095

146345497Atic

Bifunctional purine biosynthesis protein PURH0.391891742

48429104Hnrnpk

Heterogeneous nuclear ribonucleoprotein K0.390504703

85541765Blvrb

Flavin reductase (NADPH)0.387710362

14548072Ide

Insulin-degrading enzyme0.383775992

51338605Rab3c

Ras-related protein Rab-3C0.379576579

3041732Sod2

Superoxide dismutase [Mn], mitochondrial0.378816715

81901517Sdsl

Serine dehydratase-like0.375475077

37082114Pnpo

Pyridoxine-5'-phosphate oxidase0.372351321

21263432Atp5c1

ATP synthase subunit gamma, mitochondrial0.371347811

12585517Atp6v1g2

V-type proton ATPase subunit G 20.371113967

47117273Ndufs2

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial0.370835941

341940932Me1

NADP-dependent malic enzyme0.36457626

67460966Nono

Non-POU domain-containing octamer-binding protein0.361943827

47116926Lancl2

LanC-like protein 20.36057064

13431527Fibp

Acidic fibroblast growth factor intracellular-binding protein0.358478848

118572643Epn1

Epsin-10.354502462

30580818Ak3

GTP:AMP phosphotransferase, mitochondrial0.353602328

1346910Adssl1

Adenylosuccinate synthetase isozyme 10.353391981

54037163Ganab

Neutral alpha-glucosidase AB0.352800311

341940734Gucy1a3

Guanylate cyclase soluble subunit alpha-30.350361088

76364170Ddx1

ATP-dependent RNA helicase DDX10.34751402

131884Nras

GTPase NRas0.34474228

1351942Anxa5

Annexin A50.341838068

400275Map2k1

Dual specificity mitogen-activated protein kinase kinase 10.340485875

52782789Fam49b

Protein FAM49B0.340406627

41017053Clint1

Clathrin interactor 10.337929994

20137987Dclk1

Serine/threonine-protein kinase DCLK10.337669041

51317407Hpca

Neuron-specific calcium-binding protein hippocalcin0.33597829

88911344Itgad

Integrin alpha-D0.335886058

56748875Dynll2

Dynein light chain 2, cytoplasmic0.332296423

90110719Igsf8

Immunoglobulin superfamily member 80.329670488

30316201Hnrnpa3

Heterogeneous nuclear ribonucleoprotein A30.328638748

50401108Mrgpra2b

Mas-related G-protein coupled receptor member A20.326135517

2497507Dlg3

Disks large homolog 30.324775475

2493731 Clta Clathrin light chain A0.324639304

341940297Cab39

Calcium-binding protein 390.322587404

32130423Acadl

Long-chain specific acyl-CoA dehydrogenase, mitochondrial0.318806316

23503090Ndufa4

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 40.317129304

81881319Rufy3

Protein RUFY30.313158817

52783073Lsamp

Limbic system-associated membrane protein0.313132474

20141789Prdx5

Peroxiredoxin-5, mitochondrial0.306822668

61246343Snrpd3

Small nuclear ribonucleoprotein Sm D30.305360725

32469605Cap2

Adenylyl cyclase-associated protein 20.30500198

1352004Atp1b2

Sodium/potassium-transporting ATPase subunit beta-20.304512659

81869786Tenm1

Teneurin-10.304167421

2829481Idh3g

Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial0.301419643

46397834Rab7

Ras-related protein Rab-7a0.298733433

6647866 Ube3a Ubiquitin-protein ligase E3A0.294757435

51317305Gnb2

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-20.294010267

51317403Hpcal1

Hippocalcin-like protein 10.293760566

49065658Timm10

Mitochondrial import inner membrane translocase subunit Tim100.293459923

3023548Cox7b

Cytochrome c oxidase subunit 7B, mitochondrial0.292730531

17380130Nipsnap1

Protein NipSnap homolog 10.29210561

22653628Akr1a1

Alcohol dehydrogenase [NADP+]0.291261138

118105Ppia

Peptidyl-prolyl cis-trans isomerase A0.291122663

1709255Ncan

Neurocan core protein0.289424335

266414Ckmt1

Creatine kinase U-type, mitochondrial0.288098908

10719868Add1

Alpha-adducin0.287343104

341941800Rasal1

RasGAP-activating-like protein 10.284991732

341940603Anks1b

Ankyrin repeat and sterile alpha motif domain-containing protein 1B0.283604961

112984Got2

Aspartate aminotransferase, mitochondrial0.282085028

18314334Fkbp4

Peptidyl-prolyl cis-trans isomerase FKBP40.279771009

302595876Ubb

Polyubiquitin-B0.275554868

122063307Soga3

Uncharacterized protein C6orf174 homolog0.272968622

47117301Ndufb7

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 70.272939315

51704260Bsg

Basigin0.272587496

52782731Ppp2r1a

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform0.270929324

3287958Cpe

Carboxypeptidase E0.269725672

73620958Ddx3y

ATP-dependent RNA helicase DDX3Y0.268864757

55976751Atp1a1

Sodium/potassium-transporting ATPase subunit alpha-10.260110498

81886983Thumpd3

THUMP domain-containing protein 30.257366026

47117296Ndufc2

NADH dehydrogenase [ubiquinone] 1 subunit C20.255621757

6225769Nmt1

Glycylpeptide N-tetradecanoyltransferase 10.254513535

182636954Slc12a5

Solute carrier family 12 member 50.254274013

341940743Ggt7

Gamma-glutamyltransferase 70.254028019

97536879Nefl

Neurofilament light polypeptide0.249579942

51317340 Hist1h4a Histone H40.246140918

117029COX2

Cytochrome c oxidase subunit 20.245803029

66773932Basp1

Brain acid soluble protein 10.239816036

52783095Ppa1

Inorganic pyrophosphatase0.236832353

14916570G3bp2

Ras GTPase-activating protein-binding protein 20.234218138

20138411 Ptk2b Protein-tyrosine kinase 2-beta0.234207399

20140091Sfxn3

Sideroflexin-30.233659876

119348Eno2

Gamma-enolase0.232839529

121557Gpd1

Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic0.231332903

146345477Pafah1b2

Platelet-activating factor acetylhydrolase IB subunit beta0.229543832

2501665Wnt10a

Protein Wnt-10a0.228165054

81902047Lgalsl

Galectin-related protein A0.226040337

61215307 Ptprd Receptor-type tyrosine-protein phosphatase delta0.225392986

20454881Dbn1

Drebrin0.22484877

20141407Slc1a3

Excitatory amino acid transporter 10.222265994

2506545Hspa5

78 kDa glucose-regulated protein0.222001609

294862498Oxr1

Oxidation resistance protein 10.218731143

417489Pcmt1

Protein-L-isoaspartate(D-aspartate) O-methyltransferase0.217894592

81865997Ofd1

Oral-facial-digital syndrome 1 protein homolog0.214623139

24418394Aldh1l1

Cytosolic 10-formyltetrahydrofolate dehydrogenase0.211250792

126048Ldha

L-lactate dehydrogenase A chain0.211079562

25453239Ola1

Obg-like ATPase 10.210166371

56405010Eef1a1

Elongation factor 1-alpha 10.20388967

13432217Snca

Alpha-synuclein0.203300683

28376965Acot7

Cytosolic acyl coenzyme A thioester hydrolase0.20211934

78099820Atp5d

ATP synthase subunit delta, mitochondrial0.201564919

57012946Tppp

Tubulin polymerization-promoting protein0.201461234

52783579Dynll1

Dynein light chain 1, cytoplasmic0.20144008

81906185Apoo

Apolipoprotein O0.201020036

49037483Calm

Calmodulin0.20009906

1174545Syt1

Synaptotagmin-10.19973611

1352528 Notch1 Neurogenic locus notch homolog protein 10.198465028

22653715Dpp6

Dipeptidyl aminopeptidase-like protein 60.197260758

81873664Sv2b

Synaptic vesicle glycoprotein 2B0.196906512

730956Tkt

Transketolase0.196422227

48427970Abat

4-aminobutyrate aminotransferase, mitochondrial0.196045933

54039315Rps10

40S ribosomal protein S100.195297296

117128Cox7c

Cytochrome c oxidase subunit 7C, mitochondrial0.194182195

2507330Eif4a2

Eukaryotic initiation factor 4A-II0.193251912

341941109Eps15l1

Epidermal growth factor receptor substrate 15-like 10.192812717

18266844Sars

Seryl-tRNA synthetase, cytoplasmic0.190950151

68565610Idh3a

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial0.188987032

127982Nme1

Nucleoside diphosphate kinase A0.187619794

81879524Smap1

Stromal membrane-associated protein 10.187129823

3219774Prdx6

Peroxiredoxin-60.186052149

54036519Syngr3

Synaptogyrin-30.183975703

147744591 Sept3

Neuronal-specific septin-30.183910628

129392Sub1

Activated RNA polymerase II transcriptional coactivator p150.183233471

41019154Plp1

Myelin proteolipid protein0.182337594

20138723Slc25a11

Mitochondrial 2-oxoglutarate/malate carrier protein0.182232649

38258249Nt5c

5'(3')-deoxyribonucleotidase, cytosolic type0.182030617

3024764Usp5

Ubiquitin carboxyl-terminal hydrolase 50.178954494

34222626Slc25a22

Mitochondrial glutamate carrier 10.176849481

1170151Hist1h1e

Histone H1.40.176782041

51704193Vamp2

Vesicle-associated membrane protein 20.176341166

81906105Tprgl

Tumor protein p63-regulated gene 1-like protein0.175747601

9978489Hapln1

Hyaluronan and proteoglycan link protein 10.174375138

38257686Gdap1l1

Ganglioside-induced differentiation-associated protein 1-like 10.173541976

341940599Maoa

Amine oxidase [flavin-containing] A0.170741854

114393Atp1b1

Sodium/potassium-transporting ATPase subunit beta-10.170096867

14916553Dnaja2

DnaJ homolog subfamily A member 20.169230946

82592512Nckap1

Nck-associated protein 10.16626953

126042Ldhb

L-lactate dehydrogenase B chain0.164479066

22001904Rpl17

60S ribosomal protein L170.164245595

60391192Ywhae

14-3-3 protein epsilon0.163995248

52782785Sdha

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial0.162987287

113607Aldoa

Fructose-bisphosphate aldolase A0.161072856

68566306Dnm1l

Dynamin-1-like protein0.160788912

32470593Aldoc

Fructose-bisphosphate aldolase C0.158709835

10720404Vdac1

Voltage-dependent anion-selective channel protein 10.158605038

38372295Nfasc

Neurofascin0.157345496

81897852Pithd1

PITH domain-containing protein 10.157236804

14916635Pygm

Glycogen phosphorylase, muscle form0.156359407

120975Gnao1

Guanine nucleotide-binding protein G(o) subunit alpha0.15544103

22002065Rpl5

60S ribosomal protein L50.155101169

50403629Rps4x

40S ribosomal protein S4, X isoform0.153645741

81903322Acsbg1

Long-chain-fatty-acid--CoA ligase ACSBG10.152064233

55977481Tubb4b

Tubulin beta-2C chain0.149904004

81888798Ermn

Ermin0.14871394

205830666Ncam1

Neural cell adhesion molecule 10.148031728

121747Gstp1

Glutathione S-transferase P 10.147817254

128101Gap43

Neuromodulin0.14768218

68566157Ywhaq

14-3-3 protein theta0.147174095

51702275Hspa8

Heat shock cognate 71 kDa protein0.147022821

52000726 Kiaa0513 Uncharacterized protein KIAA05130.146502868

166199463Hacd3

3-hydroxyacyl-CoA dehydratase 30.145178051

81881321Ttyh1

Protein tweety homolog 10.14453373

48428644Synpo

Synaptopodin0.14435015

399833Slc2a3

Solute carrier family 2, facilitated glucose transporter member 30.142997526

30581069Sfxn5

Sideroflexin-50.140529301

73621117Phf24

Protein KIAA10450.139633044

55977057 Hist1h3a Histone H3.10.139472047

66773992Atp1a2

Sodium/potassium-transporting ATPase subunit alpha-20.139407754

51338761Cnp

2',3'-cyclic-nucleotide 3'-phosphodiesterase0.13888995

2498308Reep5

Receptor expression-enhancing protein 50.137019905

2493662Hspe1

10 kDa heat shock protein, mitochondrial0.135516459

548378Grin1

Glutamate [NMDA] receptor subunit zeta-10.134681609

341941247Bcan

Brevican core protein0.134388306

342187320Wdr47

WD repeat-containing protein 470.134169519

13124192Eef1d

Elongation factor 1-delta0.133801982

342187361Wdr7

WD repeat-containing protein 70.13067182

146291078Vcp

Transitional endoplasmic reticulum ATPase0.129654179

81903631Atp5g1

ATP synthase lipid-binding protein, mitochondrial0.129625181

52782750Atp5l

ATP synthase subunit g, mitochondrial0.129323382

59797974Arpc1a

Actin-related protein 2/3 complex subunit 1A0.129316

118542Glud1

Glutamate dehydrogenase 1, mitochondrial0.128898092

54039458Rps15a

40S ribosomal protein S15a0.128808072

55977478Tuba4a

Tubulin alpha-4A chain0.126232755

81893041Sdk2

Protein sidekick-20.125931706

13633250Prep

Prolyl endopeptidase0.125885799

30316333Gpm6b

Neuronal membrane glycoprotein M6-b0.125136332

3024586Slc1a4

Neutral amino acid transporter A0.121469599

145559539Atp6v1a

V-type proton ATPase catalytic subunit A0.120119861

22653923Palm

Paralemmin-10.118419336

47116573Actr3

Actin-related protein 30.11830822

20178035Pgam1

Phosphoglycerate mutase 10.117817327

20141656Stx1a

Syntaxin-1A0.117591951

59799776Prkacb

cAMP-dependent protein kinase catalytic subunit beta0.116793569

29427667Sirpa

Tyrosine-protein phosphatase non-receptor type substrate 10.115690236

46397725Snap25

Synaptosomal-associated protein 250.11503382

17380315Napa

Alpha-soluble NSF attachment protein0.114411395

19862081Uqcrh

Cytochrome b-c1 complex subunit 6, mitochondrial0.113214584

1703188Slc25a5

ADP/ATP translocase 20.1124402

115311320Camkv

CaM kinase-like vesicle-associated protein0.110392689

14917005 Hspa9 Stress-70 protein, mitochondrial0.110212591

47117288Rap1b

Ras-related protein Rap-1b0.109566352

341941972Arhgap5

Rho GTPase-activating protein 50.109196311

48428679Oxct1

Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial0.108020345

50402098Eef1a2

Elongation factor 1-alpha 20.107831136

21363012Trf

Serotransferrin0.106800601

46396509Pdhb

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial0.106128114

322510016Cdc42

Cell division control protein 42 homolog0.105505616

17378829Mbp

Myelin basic protein0.104084057

20141804 Ppp5c Serine/threonine-protein phosphatase 50.103689339

341940511Dctn1

Dynactin subunit 10.10231559

46577661Ube2n

Ubiquitin-conjugating enzyme E2 N0.100859452

81879688Pip4k2c

Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma0.100201636

46397704Rps20

40S ribosomal protein S200.098441117

353526354Tpi1

Triosephosphate isomerase0.098164838

32130449Rpl12

60S ribosomal protein L120.097183197

116918Cntn1

Contactin-10.096179411

81879424Hnrnpu

Heterogeneous nuclear ribonucleoprotein U0.095243336

61217662Ddx39b

Spliceosome RNA helicase Ddx39b0.095044798

48428722Ywhag

14-3-3 protein gamma0.093046308

121430Gria1

Glutamate receptor 10.092496878

205830867Nfu1

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial0.091755338

73919260Dync1li1

Cytoplasmic dynein 1 light intermediate chain 10.090362891

152031595Dmxl2

DmX-like protein 20.088558323

38258292Rem1

GTP-binding protein REM 10.088201899

117502Calr

Calreticulin0.088059467

81901032Paf1

RNA polymerase II-associated factor 1 homolog0.087209628

3023546Cox6b1

Cytochrome c oxidase subunit 6B10.086891242

547881Gpm6a

Neuronal membrane glycoprotein M6-a0.086380174

119362Hsp90b1

Endoplasmin0.085909161

341940485Ecm1

Extracellular matrix protein 10.084587724

399310Ctnnb1

Catenin beta-10.084463235

146345470Nsf

Vesicle-fusing ATPase0.083866419

42559891Srcin1

SRC kinase signaling inhibitor 10.083711105

13637776Eno1

Alpha-enolase0.083374649

20178348 Sept6

Septin-60.082111075

81896262Iars2

Isoleucyl-tRNA synthetase, mitochondrial0.08044518

54036496Tmsb10

Thymosin beta-100.079695809

41019466Syp

Synaptophysin0.079004491

55976720Trim46

Tripartite motif-containing protein 460.078793023

32172431Dnm1

Dynamin-10.078494053

54038800Ppp3ca

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform0.077031999

146345481Pgk1

Phosphoglycerate kinase 10.076502521

14916528Add2

Beta-adducin0.076271648

341940235Anxa7

Annexin A70.076222283

118572640Dld

Dihydrolipoyl dehydrogenase, mitochondrial0.076202583

341941236Pde1a

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A0.075234686

13959400Ak1

Adenylate kinase isoenzyme 10.07491936

20455323Tubb3

Tubulin beta-3 chain0.07326884

1709972Rpl10a

60S ribosomal protein L10a0.072920532

52001076Mapk1

Mitogen-activated protein kinase 10.072560801

8928228Ndrg2

Protein NDRG20.072533209

34395652 Rapgef2 Rap guanine nucleotide exchange factor 20.07097247

112804Slc3a2

4F2 cell-surface antigen heavy chain0.070362554

59798433Polk

DNA polymerase kappa0.070339284

52783455Aldh5a1

Succinate-semialdehyde dehydrogenase, mitochondrial0.069014306

22256950Pacsin1

Protein kinase C and casein kinase substrate in neurons protein 10.068683504

146345457Mdh2

Malate dehydrogenase, mitochondrial0.068177466

341941270Plcb1

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-10.067093927

51702779Pfn1

Profilin-10.065753344

13629369Pfkp

6-phosphofructokinase type C0.065717237

67460582Gpr37l1

Endothelin B receptor-like protein 20.064681879

9910830Psma4

Proteasome subunit alpha type-40.064590021

1729865Tcp1

T-complex protein 1 subunit alpha0.064035584

3914439Psmb4

Proteasome subunit beta type-40.063177511

110825706Arpc2

Actin-related protein 2/3 complex subunit 20.060567028

52000885Ywhaz

14-3-3 protein zeta/delta0.060452222

57012721Cs

Citrate synthase, mitochondrial0.060289474

341941780Uqcrc1

Cytochrome b-c1 complex subunit 1, mitochondrial0.060194386

20455200Ppp2r4

Serine/threonine-protein phosphatase 2A activator0.06019164

51338651Rps26

40S ribosomal protein S260.059927678

12643614Atp2a2

Sarcoplasmic/endoplasmic reticulum calcium ATPase 20.058964786

90110042Osbpl1a

Oxysterol-binding protein-related protein 10.058860554

22001911Rpl4

60S ribosomal protein L40.058818739

341941040Kbtbd11

Kelch repeat and BTB domain-containing protein 110.057849345

20454828Atp5f1

ATP synthase subunit b, mitochondrial0.055944404

166200058Rogdi

Protein rogdi homolog0.054566945

1350822Hnrnpa1

Heterogeneous nuclear ribonucleoprotein A10.053986481

2506252Pdcd6

Programmed cell death protein 60.053855247

24638218Sh3bgrl3

SH3 domain-binding glutamic acid-rich-like protein 30.053322267

13123946Actn4

Alpha-actinin-40.053077597

83305642 Sept5

Septin-50.051545159

47117291Ndufs5

NADH dehydrogenase [ubiquinone] iron-sulfur protein 50.05150326

18266783Copb2

Coatomer subunit beta'0.05058272

189045497Ccdc173

Uncharacterized protein C2orf77 homolog0.050559089

20138857Fxyd1

Phospholemman0.049583377

21903382Slc25a4

ADP/ATP translocase 10.048057635

71153505Dhx9

ATP-dependent RNA helicase A0.04662112

18202285Eef2

Elongation factor 20.045676951

20178336Tpm3

Tropomyosin alpha-3 chain0.045633732

66773801Cltc

Clathrin heavy chain 10.045109747

81871880Arhgap44

Rho GTPase-activating protein 440.042415487

10719959Cpne6

Copine-60.042103215

116243094Ckap4

Cytoskeleton-associated protein 40.041924119

341940992Mink1

Misshapen-like kinase 10.041803392

341942067Cttn

Src substrate cortactin0.041509649

47117840Napb

Beta-soluble NSF attachment protein0.040813747

134614Sod1

Superoxide dismutase [Cu-Zn]0.040613235

52000687Atp1a3

Sodium/potassium-transporting ATPase subunit alpha-30.040132628

62287163Necap1

Adaptin ear-binding coat-associated protein 10.039933509

47117625Atp6v1f

V-type proton ATPase subunit F0.039069772

54039312Rps13

40S ribosomal protein S130.037702112

67460489Cand1

Cullin-associated NEDD8-dissociated protein 10.037617472

55977479Tuba1a

Tubulin alpha-1A chain0.037456275

47605546Atp5o

ATP synthase subunit O, mitochondrial0.03745236

341941063Hk1

Hexokinase-10.037034781

32363402Ndufa8

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 80.034131689

1346597Atoh1

Protein atonal homolog 10.032731835

146291096Atp6v1c1

V-type proton ATPase subunit C 10.032633738

2495342Hspa4

Heat shock 70 kDa protein 40.031702587

21759079Cyc1

Cytochrome c1, heme protein, mitochondrial0.031651193

52788305Sucla2

Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial0.030361057

60391212Aco2

Aconitate hydratase, mitochondrial0.02893157

298286902Nptn

Neuroplastin0.02858903

29427692Immt

Mitochondrial inner membrane protein0.028161076

122028Hist1h2bm

Histone H2B type 1-M0.027281113

22095581Dctn2

Dynactin subunit 20.02671361

81878911H2afj

Histone H2A.J0.024939025

46577330Hnrnph2

Heterogeneous nuclear ribonucleoprotein H20.024782209

145559451Cbr1

Carbonyl reductase [NADPH] 10.024433208

25453322Vps35

Vacuolar protein sorting-associated protein 350.023269075

52783422Slc38a3

Sodium-coupled neutral amino acid transporter 30.022942536

47117242Ndufs8

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial0.022915962

341942137Slc26a5

Prestin0.021835381

51317303Gnb1

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-10.021264933

146345448Pkm

Pyruvate kinase isozymes M1/M20.021121521

117949769Pcsk1n

ProSAAS0.020593223

20455479Atp5b

ATP synthase subunit beta, mitochondrial0.019419214

17380333Uqcrb

Cytochrome b-c1 complex subunit 70.01765482

47117308Ndufb8

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial0.017366645

59798463Lin7a

Protein lin-7 homolog A0.017193824

45477158Acsl6

Long-chain-fatty-acid--CoA ligase 60.017155347

1172773Pura

Transcriptional activator protein Pur-alpha0.016998457

62296680Rpl6

60S ribosomal protein L60.01696029

223634791Ank2

Ankyrin-20.016728273

25089776Atp5h

ATP synthase subunit d, mitochondrial0.016047685

52783213Serbp1

Plasminogen activator inhibitor 1 RNA-binding protein0.014876848

41016844Amph

Amphiphysin0.012007681

416677Atp5a1

ATP synthase subunit alpha, mitochondrial0.011477322

66773987Krt77

Keratin, type II cytoskeletal 1b0.010497233

223634732Suclg1

Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial0.010418094

205830863Map6

Microtubule-associated protein 60.010233724

13432200Mapt

Microtubule-associated protein tau0.009647737

341940935Map2

Microtubule-associated protein 20.00963116

48429206Stxbp1

Syntaxin-binding protein 10.009471844

8928560Cox7a2

Cytochrome c oxidase subunit 7A2, mitochondrial0.009201831

44888293Pdxp

Pyridoxal phosphate phosphatase0.008245011

341940637Cap1

Adenylyl cyclase-associated protein 10.008236539

338817898Got1

Aspartate aminotransferase, cytoplasmic0.005785987

54036698Actc1

Actin, alpha cardiac muscle 10.004112246

543921Canx

Calnexin0.00348337

51702788Rac1

Ras-related C3 botulinum toxin substrate 10.003225187

54039390Rps17

40S ribosomal protein S170.002675657

47117658Arf3

ADP-ribosylation factor 30.000275041

146345462Ndufs3

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial-6.84711E-05

548409Pdha1

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial-0.000717312

81862370Cyfip2

Cytoplasmic FMR1-interacting protein 2-0.00111654

341942263Prps1

Ribose-phosphate pyrophosphokinase 1-0.001799497

146345383Car2

Carbonic anhydrase 2-0.002317924

81867523Lrp1

Prolow-density lipoprotein receptor-related protein 1-0.00248415

52000877Uqcrfs1

Cytochrome b-c1 complex subunit Rieske, mitochondrial-0.002872224

81870005Ncdn

Neurochondrin-0.003049876

146345422Gpi1

Glucose-6-phosphate isomerase-0.005572129

124056467Camk2a

Calcium/calmodulin-dependent protein kinase type II subunit alpha-0.005761008

68565939Nudt3

Diphosphoinositol polyphosphate phosphohydrolase 1-0.006346265

47117649Actr2

Actin-related protein 2-0.006553535

46397808Coro1a

Coronin-1A-0.007166607

1169460Slc1a2

Excitatory amino acid transporter 2-0.007531097

22654291Cct2

T-complex protein 1 subunit beta-0.008754924

46577639Rab10

Ras-related protein Rab-10-0.010222192

120223Fkbp1a

Peptidyl-prolyl cis-trans isomerase FKBP1A-0.010916835

81893945Nadsyn1

Glutamine-dependent NAD(+) synthetase-0.011804297

73920802Syn1

Synapsin-1-0.011947181

17380576Tspan7

Tetraspanin-7-0.012843921

46576352Cacna2d1

Voltage-dependent calcium channel subunit alpha-2/delta-1-0.013089888

51702234Cycs

Cytochrome c, somatic-0.014055481

119370262Anp32-ps

Putative acidic leucine-rich nuclear phosphoprotein 32 family member C-0.014651995

125987842Myo18a

Myosin-XVIIIa-0.016400725

134047901Rtn3

Reticulon-3-0.016511142

129261Spp1

Osteopontin-0.016972056

122065442Map1a

Microtubule-associated protein 1A-0.017196441

21759257Slc25a3

Phosphate carrier protein, mitochondrial-0.017786205

81905494Fam162a

Protein FAM162A-0.018208478

81881914Sv2a

Synaptic vesicle glycoprotein 2A-0.018694473

341940472Dync1h1

Cytoplasmic dynein 1 heavy chain 1-0.020587804

44888264Otub1

Ubiquitin thioesterase OTUB1-0.022488211

1350769Rpl7

60S ribosomal protein L7-0.022981122

1346207Gstm5

Glutathione S-transferase Mu 5-0.025827651

417208Ckb

Creatine kinase B-type-0.026557083

46397828Ranbp1

Ran-specific GTPase-activating protein-0.027334027

3023549Cox17

Cytochrome c oxidase copper chaperone-0.027713226

109818808Dnajc6

Putative tyrosine-protein phosphatase auxilin-0.028105426

30913117Scrn1

Secernin-1-0.028442062

17380314Napg

Gamma-soluble NSF attachment protein-0.028506677

17432986 Epb41l2 Band 4.1-like protein 2-0.028636121

135835Ptma

Prothymosin alpha-0.028804805

13638207Pfkm

6-phosphofructokinase, muscle type-0.029403237

94730394Camk2b

Calcium/calmodulin-dependent protein kinase type II subunit beta-0.029815784

54038837Phb

Prohibitin-0.029986588

547923Prdx1

Peroxiredoxin-1-0.030661958

41688724Mrps36

28S ribosomal protein S36, mitochondrial-0.031972473

341941110Ep400

E1A-binding protein p400-0.032080254

52000832Acat1

Acetyl-CoA acetyltransferase, mitochondrial-0.032510948

150438863Cadm1

Cell adhesion molecule 1-0.033270848

97537229Sptbn1

Spectrin beta chain, brain 1-0.033565486

47117767Stx1b

Syntaxin-1B-0.033887854

134047752Epb4.1l1

Band 4.1-like protein 1-0.034786118

81882150Ap3d1

AP-3 complex subunit delta-1-0.036566318

341940296Casp8ap2

CASP8-associated protein 2-0.037173946

12585446Atp6v1d

V-type proton ATPase subunit D-0.037459094

92087001Mdh1

Malate dehydrogenase, cytoplasmic-0.03780606

1170384Hsp90aa1

Heat shock protein HSP 90-alpha-0.038214291

122066202Sptan1

Spectrin alpha chain, brain-0.039348698

51317306Gnb5

Guanine nucleotide-binding protein subunit beta-5-0.040305854

81905699Ociad2

OCIA domain-containing protein 2-0.041205223

59797879Dlg2

Disks large homolog 2-0.041418597

3913376Crym

Thiomorpholine-carboxylate dehydrogenase-0.041898263

117066mt-Co3

Cytochrome c oxidase subunit 3-0.04299442

81885886Nap1l4

Nucleosome assembly protein 1-like 4-0.04375094

416827Cox4i1

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial-0.046129587

21362640Glo1

Lactoylglutathione lyase-0.047680305

20138079Epb4.1l3

Band 4.1-like protein 3-0.049746136

56749655 Sept9

Septin-9-0.050869417

54036535Skp1a

S-phase kinase-associated protein 1-0.051825862

13124470Pfn2

Profilin-2-0.055005083

62288910Rhpn2

Rhophilin-2-0.056737829

94730353Ina

Alpha-internexin-0.059156246

1708288Hprt

Hypoxanthine-guanine phosphoribosyltransferase-0.061421496

54036445Stip1

Stress-induced-phosphoprotein 1-0.06145064

61229841Pdxk

Pyridoxal kinase-0.061574589

78099781Itpr3

Inositol 1,4,5-trisphosphate receptor type 3-0.062830169

1345668Capzb

F-actin-capping protein subunit beta-0.063095752

81894445Smap2

Stromal membrane-associated protein 2-0.064458149

54039385Rps18

40S ribosomal protein S18-0.065982773

341940423Dync1i1

Cytoplasmic dynein 1 intermediate chain 1-0.066537845

266608Nme2

Nucleoside diphosphate kinase B-0.066616591

32363396Ndufa2

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2-0.0680427

341940931Me3

NADP-dependent malic enzyme, mitochondrial-0.068287871

67460396Tufm

Elongation factor Tu, mitochondrial-0.068865018

1711560Stmn1

Stathmin-0.069016168

1351911Akr1b3

Aldose reductase-0.070282308

41017503Nsfl1c

NSFL1 cofactor p47-0.070986963

341940983Myo5a

Myosin-Va-0.071977993

20141353Gsta4

Glutathione S-transferase A4-0.073181873

145559476Glul

Glutamine synthetase-0.074450395

146345468Nefm

Neurofilament medium polypeptide-0.074637267

117104Cox5b

Cytochrome c oxidase subunit 5B, mitochondrial-0.075399763

3024089Lasp1

LIM and SH3 domain protein 1-0.075535795

18203578Ehd1

EH domain-containing protein 1-0.075567787

14548302Uqcrc2

Cytochrome b-c1 complex subunit 2, mitochondrial-0.077725396

46396900Pygb

Glycogen phosphorylase, brain form-0.077738069

166897986Cox5a

Cytochrome c oxidase subunit 5A, mitochondrial-0.077756746

464506Pcx

Pyruvate carboxylase, mitochondrial-0.078713694

61216668Rtn1

Reticulon-1-0.078866881

135831Thy1

Thy-1 membrane glycoprotein-0.079175856

97051843Gprin1

G protein-regulated inducer of neurite outgrowth 1-0.07976062

94730376Dpysl2

Dihydropyrimidinase-related protein 2-0.081453433

150438864Cadm2

Cell adhesion molecule 2-0.081844618

47116230Grhpr

Glyoxylate reductase/hydroxypyruvate reductase-0.0819569

18202309Opa1

Dynamin-like 120 kDa protein, mitochondrial-0.082162201

14549168Grin2b

Glutamate [NMDA] receptor subunit epsilon-2-0.082487367

14285350Atp2b2

Plasma membrane calcium-transporting ATPase 2-0.083538802

6707708Ak4

Adenylate kinase isoenzyme 4, mitochondrial-0.084008751

9789726 Sept7

Septin-7-0.084394205

341940212Rnpep

Aminopeptidase B-0.084943466

125199Prkar2a

cAMP-dependent protein kinase type II-alpha regulatory subunit-0.08495461

46395721Actn1

Alpha-actinin-1-0.085028664

342187144Atp6v0a1

V-type proton ATPase 116 kDa subunit a isoform 1-0.08548954

38372441Nrcam

Neuronal cell adhesion molecule-0.088633663

62510833Dlst

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial -0.088841253

68775966Tubb6

Tubulin beta-6 chain-0.089545815

1352217Gad2

Glutamate decarboxylase 2-0.09003141

60390186Mapre3

Microtubule-associated protein RP/EB family member 3-0.091435264

46396175Nrgn

Neurogranin-0.091894624

54037693Prkcg

Protein kinase C gamma type-0.091996453

38258917Rab5c

Ras-related protein Rab-5C-0.092073085

55976655Ywhah

14-3-3 protein eta-0.092342244

1729962Tst

Thiosulfate sulfurtransferase-0.09237535

254763263Coro2b

Coronin-2B-0.092778067

341940631Arhgef2

Rho guanine nucleotide exchange factor 2-0.093849653

342187037Tnr

Tenascin-R-0.093985125

341942109Spg11

Spatacsin-0.094001487

341940634Bsn

Protein bassoon-0.094059599

2506794Kif1a

Kinesin-like protein KIF1A-0.094804007

81911483Camk2d

Calcium/calmodulin-dependent protein kinase type II subunit delta-0.095411286

2492687Snap91

Clathrin coat assembly protein AP180-0.096853077

121716Gstm1

Glutathione S-transferase Mu 1-0.098550049

31077176Hspa4l

Heat shock 70 kDa protein 4L-0.101299837

23396478Mpc2

Brain protein 44-0.101338328

143811473Atp6v1e1

V-type proton ATPase subunit E 1-0.102812833

84029467Slc4a4

Electrogenic sodium bicarbonate cotransporter 1-0.103739146

21759130Arhgdia

Rho GDP-dissociation inhibitor 1-0.104212409

20139162Ndufa13

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-0.104619737

32469783Lgi1

Leucine-rich glioma-inactivated protein 1-0.10586298

47606041Phyhip

Phytanoyl-CoA hydroxylase-interacting protein-0.106399298

267190Uba1

Ubiquitin-like modifier-activating enzyme 1-0.107177059

78099814Atp6v1h

V-type proton ATPase subunit H-0.107951773

145566796Myl12b

Myosin regulatory light chain 12B-0.10949155

549057Cct4

T-complex protein 1 subunit delta-0.111749452

51338706Atp6v1b2

V-type proton ATPase subunit B, brain isoform-0.112256788

146345423Gfap

Glial fibrillary acidic protein-0.112307383

341940436Ddx5

Probable ATP-dependent RNA helicase DDX5-0.113352596

160406731Sh3gl2

Endophilin-A1-0.114946928

341942254Pgm2

Phosphoglucomutase-1-0.115097836

1709998Rab2a

Ras-related protein Rab-2A-0.115782604

22653814Mapk8

Mitogen-activated protein kinase 8-0.115914925

143955288Mcu

Calcium uniporter protein, mitochondrial-0.116192866

20141252Atp5j2

ATP synthase subunit f, mitochondrial-0.116587926

51338599Ran

GTP-binding nuclear protein Ran-0.118388211

51316977Ap2m1

AP-2 complex subunit mu-0.122134751

2498751Pea15a

Astrocytic phosphoprotein PEA-15-0.122831995

341941260Pik3cd

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform-0.125550004

54037523Csnk2b

Casein kinase II subunit beta-0.12739058

3122044Dpysl4

Dihydropyrimidinase-related protein 4-0.129591994

49065818Psmc1

26S protease regulatory subunit 4-0.129969654

41017259Mok

MAPK/MAK/MRK overlapping kinase-0.133435691

81900953Coq9

Ubiquinone biosynthesis protein COQ9, mitochondrial-0.134532656

51701351Ap2b1

AP-2 complex subunit beta-0.13474657

32363397Ndufb4

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4-0.136388841

23396508Cndp2

Cytosolic non-specific dipeptidase-0.136562078

52783747Hmgb1

High mobility group protein B1-0.138360843

32363403Ndufb10

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-0.139186642

24212071Tomm22

Mitochondrial import receptor subunit TOM22 homolog-0.139466997

160409932Sh3glb2

Endophilin-B2-0.140263712

52783229Psmd11

26S proteasome non-ATPase regulatory subunit 11-0.141102265

52001083Pcp4

Purkinje cell protein 4-0.142849438

81881592Arpc5l

Actin-related protein 2/3 complex subunit 5-like protein-0.143648878

341940933Map1b

Microtubule-associated protein 1B-0.144603351

114041Apoe

Apolipoprotein E-0.145567789

1352173Cox6a1

Cytochrome c oxidase subunit 6A1, mitochondrial-0.14563512

124028629Hnrnpa2b1

Heterogeneous nuclear ribonucleoproteins A2/B1-0.146154084

9910832Psmb2

Proteasome subunit beta type-2-0.14617781

146291087Tmod2

Tropomodulin-2-0.14691154

47605664Hapln4

Hyaluronan and proteoglycan link protein 4-0.147122922

50400980Lrrc7

Leucine-rich repeat-containing protein 7-0.148599827

108935937Tceal3

Transcription elongation factor A protein-like 3-0.152910085

113334Ap2a1

AP-2 complex subunit alpha-1-0.154393298

38604983Abhd12

Monoacylglycerol lipase ABHD12-0.15613578

20140777Uqcrq

Cytochrome b-c1 complex subunit 8-0.157366745

60390207Mapre2

Microtubule-associated protein RP/EB family member 2-0.160265171

146345384Ppp3r1

Calcineurin subunit B type 1-0.16060945

21759002Ndufab1

Acyl carrier protein, mitochondrial-0.161469758

46396655Set

Protein SET-0.162684603

341942266Pygl

Glycogen phosphorylase, liver form-0.164425575

51701449Sdhb

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial-0.167663046

5915682Alb

Serum albumin-0.168028339

47117166Ndufa12

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12-0.16910016

52783594Gng2

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2-0.169959192

549059Cct3

T-complex protein 1 subunit gamma-0.170892316

146345463Ndufv2

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial-0.171808434

3334470Mag

Myelin-associated glycoprotein-0.173455158

146345407Ctbp1

C-terminal-binding protein 1-0.173530011

116248181Fam131b

Protein FAM131B-0.175087636

73920803Syn2

Synapsin-2-0.175100716

51316667Rps27

40S ribosomal protein S27-0.176264283

39931169Bphl

Valacyclovir hydrolase-0.178913876

32699478Nvl

Nuclear valosin-containing protein-like-0.179387453

51316976Ap2s1

AP-2 complex subunit sigma-0.179529465

47605479Slc25a12

Calcium-binding mitochondrial carrier protein Aralar1-0.180608948

341941065Hsp90ab1

Heat shock protein HSP 90-beta-0.181119834

146345480Pdia3

Protein disulfide-isomerase A3-0.18232729

62510940Letm1

LETM1 and EF-hand domain-containing protein 1, mitochondrial-0.183442078

51338653Rps28

40S ribosomal protein S28-0.184366885

94730399Nefh

Neurofilament heavy polypeptide-0.185877179

78103425Wasf1

Wiskott-Aldrich syndrome protein family member 1-0.189259153

81901722Snap47

Synaptosomal-associated protein 47-0.189817756

32363398Ndufa6

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6-0.190498675

20138335Gng12

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12-0.190869347

122441Hba-a1

Hemoglobin subunit alpha-0.192846289

46577115Ndufb9

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9-0.193324342

51702798Rab3a

Ras-related protein Rab-3A-0.196672064

147721201Ppm1e

Protein phosphatase 1E-0.198211362

110279022Iqsec1

IQ motif and SEC7 domain-containing protein 1-0.19840257

13626388Eef1g

Elongation factor 1-gamma-0.199512608

251757493Gnaq

Guanine nucleotide-binding protein G(q) subunit alpha-0.19953074

2497313Mog

Myelin-oligodendrocyte glycoprotein-0.200679512

81898294Vps26b

Vacuolar protein sorting-associated protein 26B-0.200783874

146325019Ogt

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit-0.201585488

23822162Snta1

Alpha-1-syntrophin-0.202134143

81875980Asrgl1

L-asparaginase-0.202772672

81886139Cep120

Centrosomal protein of 120 kDa-0.202810921

120702Gapdh

Glyceraldehyde-3-phosphate dehydrogenase-0.203425466

14916536Bin1

Myc box-dependent-interacting protein 1-0.203575911

46397334Actb

Actin, cytoplasmic 1-0.203709208

94730421Rtn4

Reticulon-4-0.204840557

398990Dbi

Acyl-CoA-binding protein-0.205931285

9910725Gda

Guanine deaminase-0.207839279

68566057Sfpq

Splicing factor, proline- and glutamine-rich-0.20821486

2501457Usp17la

Ubiquitin carboxyl-terminal hydrolase DUB-1-0.210905157

12643842Acot9

Acyl-coenzyme A thioesterase 9, mitochondrial-0.212458413

3122030Crmp1

Dihydropyrimidinase-related protein 1-0.213192343

51316996Cplx2

Complexin-2-0.216017143

120982Gna11

Guanine nucleotide-binding protein subunit alpha-11-0.21882669

22653706Dmtn

Dematin-0.21891236

27923753Cgn

Cingulin-0.219557341

341940817Idh1

Isocitrate dehydrogenase [NADP] cytoplasmic-0.220904089

334305788Gnai1

Guanine nucleotide-binding protein G(i) subunit alpha-1-0.221128705

341941131Pfkl

6-phosphofructokinase, liver type-0.221320207

146345417Etfa

Electron transfer flavoprotein subunit alpha, mitochondrial-0.221497211

341941160Ndufs1

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial-0.223196501

341941159Ndufa9

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial-0.22557774

81902092Agap3

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3-0.227203596

254765198Tagap

T-cell activation Rho GTPase-activating protein-0.229978151

1709737Pitpna

Phosphatidylinositol transfer protein alpha isoform-0.230089253

56404944Park7

Protein DJ-1-0.233870392

8928249Ptges3

Prostaglandin E synthase 3-0.234938931

81895995Enoph1

Enolase-phosphatase E1-0.235214114

52000925Kcna2

Potassium voltage-gated channel subfamily A member 2-0.235953156

38257365Gdap1

Ganglioside-induced differentiation-associated protein 1-0.235970203

3929369 Srsf5 Serine/arginine-rich splicing factor 5-0.23808411

334302763 Ankdd1b Ankyrin repeat and death domain-containing protein 1B-0.23852631

341941093Fh1

Fumarate hydratase, mitochondrial-0.241220073

47117855Gdi1

Rab GDP dissociation inhibitor alpha-0.242261309

341941223Pabpc1

Polyadenylate-binding protein 1-0.242417873

341940231Ap2a2

AP-2 complex subunit alpha-2-0.244482929

3334247C1qbp

Complement component 1 Q subcomponent-binding protein, mitochondrial-0.244893508

18203410Uchl1

Ubiquitin carboxyl-terminal hydrolase isozyme L1-0.250921366

54040727Fasn

Fatty acid synthase-0.251011733

341941239P4hb

Protein disulfide-isomerase-0.25298495

115503759Aak1

AP2-associated protein kinase 1-0.25341696

27734429Mpp2

MAGUK p55 subfamily member 2-0.255822641

124056472Kifc3

Kinesin-like protein KIFC3-0.255888843

1346600Mtx1

Metaxin-1-0.255992104

50401075Itpka

Inositol-trisphosphate 3-kinase A-0.256167524

51703309Rps3

40S ribosomal protein S3-0.256571609

341942264Psmd12

26S proteasome non-ATPase regulatory subunit 12-0.256899737

146345472Ogdh

2-oxoglutarate dehydrogenase, mitochondrial-0.257672587

6685313Cldn11

Claudin-11-0.261591239

150421540Dnajb6

DnaJ homolog subfamily B member 6-0.262523443

20140237Efhd2

EF-hand domain-containing protein D2-0.263293993

13626886Gdi2

Rab GDP dissociation inhibitor beta-0.263502634

51316454Hpcal4

Hippocalcin-like protein 4-0.263562532

549060Cct7

T-complex protein 1 subunit eta-0.264017447

66773929Rcn2

Reticulocalbin-2-0.268465949

51702252Hspd1

60 kDa heat shock protein, mitochondrial-0.268540392

21431839Rph3a

Rabphilin-3A-0.270232179

6166129Cryab

Alpha-crystallin B chain-0.271675093

81881843Aldh6a1

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial-0.272285087

342187017Slc17a7

Vesicular glutamate transporter 1-0.27388924

57012952Pdhx

Pyruvate dehydrogenase protein X component, mitochondrial-0.274976827

300669660Npepps

Puromycin-sensitive aminopeptidase-0.277521769

81894883Ahcyl1

Putative adenosylhomocysteinase 2-0.278450858

341940405Csnk2a1

Casein kinase II subunit alpha-0.280036751

3122052Dpysl3

Dihydropyrimidinase-related protein 3-0.284246807

46395722Ctdp1

RNA polymerase II subunit A C-terminal domain phosphatase-0.286347696

18202836Ywhab

14-3-3 protein beta/alpha-0.288267439

146345529Tubb4a

Tubulin beta-4 chain-0.290874932

1710815S100b

Protein S100-B-0.292282052

30913410Ubxn6

UBX domain-containing protein 6-0.293945544

8134844Slc30a3

Zinc transporter 3-0.297714753

55977306Psmc6

26S protease regulatory subunit 10B-0.303869118

341940560Cadps

Calcium-dependent secretion activator 1-0.306141601

46576875Rab8b

Ras-related protein Rab-8B-0.31012696

6093647Pak1

Serine/threonine-protein kinase PAK 1-0.310687973

33112324Hspa12a

Heat shock 70 kDa protein 12A-0.312534564

54041237Prkar2b

cAMP-dependent protein kinase type II-beta regulatory subunit-0.314898785

1345696Capza2

F-actin-capping protein subunit alpha-2-0.315056287

81882145Pitpnm1

Membrane-associated phosphatidylinositol transfer protein 1-0.315184306

83305135Ndufs6

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial-0.316179045

38258618Sirt2

NAD-dependent deacetylase sirtuin-2-0.318787877

125550Prkca

Protein kinase C alpha type-0.319603222

71152969Myh10

Myosin-10-0.31999929

341941233Pccb

Propionyl-CoA carboxylase beta chain, mitochondrial-0.327694452

231557Apoa1

Apolipoprotein A-I-0.329998472

341940825Ikzf3

Zinc finger protein Aiolos-0.331366817

342187059Tomm70a

Mitochondrial import receptor subunit TOM70-0.332345378

2500582Npm1

Nucleophosmin-0.333362229

56754803Tubb5

Tubulin beta-5 chain-0.338428655

51702142Ube2v2

Ubiquitin-conjugating enzyme E2 variant 2-0.339452999

32363386Ndufa10

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial-0.344575197

134035345Ube2z

Ubiquitin-conjugating enzyme E2 Z-0.344682331

41017420Lypla2

Acyl-protein thioesterase 2-0.351617572

73921210Ndfip2

NEDD4 family-interacting protein 2-0.3588888

62510502 Chchd6 Coiled-coil-helix-coiled-coil-helix domain-containing protein 6-0.361072338

81903636Fam136a

Protein FAM136A-0.362851338

341940371Cntnap1

Contactin-associated protein 1-0.363527701

114151682Alkbh5

Probable alpha-ketoglutarate-dependent dioxygenase ABH5-0.366359695

21759077Cotl1

Coactosin-like protein-0.367542608

52001483Mapk3

Mitogen-activated protein kinase 3-0.368671842

138536Vim

Vimentin-0.373062493

47117040Mgll

Monoglyceride lipase-0.380835896

12229953Psma5

Proteasome subunit alpha type-5-0.381067896

47606029Pip4k2b

Phosphatidylinositol-5-phosphate 4-kinase type-2 beta-0.38111917

42559420Pcbp1

Poly(rC)-binding protein 1-0.381540619

110825702Adh5

Alcohol dehydrogenase class-3-0.382830308

23831273Ppp1r1b

Protein phosphatase 1 regulatory subunit 1B-0.383298173

2499458Nptx1

Neuronal pentraxin-1-0.391257945

52783239Rpl14

60S ribosomal protein L14-0.391972163

38372626Arpc4

Actin-related protein 2/3 complex subunit 4-0.392214643

50400892Kctd12

BTB/POZ domain-containing protein KCTD12-0.396546632

12230747Wdr1

WD repeat-containing protein 1-0.402813693

51316035Blzf1

Golgin-45-0.405659682

17380131Gbas

Protein NipSnap homolog 2-0.410109796

51338697Vsnl1

Visinin-like protein 1-0.414033605

341940828Kpnb1

Importin subunit beta-1-0.425861051

47117859Aqp4

Aquaporin-4-0.427168917

81910090Tom1l2

TOM1-like protein 2-0.438950356

46577316Ube2d1

Ubiquitin-conjugating enzyme E2 D1-0.439389499

110816418Slirp

SRA stem-loop-interacting RNA-binding protein, mitochondrial-0.440310503

341940488Eif3f

Eukaryotic translation initiation factor 3 subunit F-0.443976318

132626Rrm2

Ribonucleoside-diphosphate reductase subunit M2-0.444061095

45476974Ddah1

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1-0.447638587

18203409Dstn

Destrin-0.449411949

152031548Mllt4

Afadin-0.452231111

9910829Psma6

Proteasome subunit alpha type-6-0.452778456

126752Marcks

Myristoylated alanine-rich C-kinase substrate-0.456751528

81901922Sgip1

SH3-containing GRB2-like protein 3-interacting protein 1-0.460581434

21362536Dpysl5

Dihydropyrimidinase-related protein 5-0.465184953

47606442Myl6

Myosin light polypeptide 6-0.478918517

51316048Epm2aip1

EPM2A-interacting protein 1-0.485083143

46576641Cacybp

Calcyclin-binding protein-0.487723192

338817851Add3

Gamma-adducin-0.491204536

50401563 Sept11

Septin-11-0.492066464

97046551Agap2

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2-0.497694153

117949796Tenm4

Teneurin-4-0.499963014

46396281Kcnma1

Calcium-activated potassium channel subunit alpha-1-0.5032319

47117298Ndufb5

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial-0.510030052

20981714Syngr1

Synaptogyrin-1-0.522227011

51315842Dbnl

Drebrin-like protein-0.525557084

62510660Cyb5b

Cytochrome b5 type B-0.527305635

81892818Ppp1r9b

Neurabin-2-0.531211744

20141319Gnaz

Guanine nucleotide-binding protein G(z) subunit alpha-0.532824867

17368615Mlf2

Myeloid leukemia factor 2-0.533711592

20978552Psmd6

26S proteasome non-ATPase regulatory subunit 6-0.537390416

341941148Gnai2

Guanine nucleotide-binding protein G(i) subunit alpha-2-0.541814826

341940408Cspg5

Chondroitin sulfate proteoglycan 5-0.5562479

85700953Pdss1

Decaprenyl-diphosphate synthase subunit 1-0.558598176

122513Hbb-b1

Hemoglobin subunit beta-1-0.561727314

2498952Spr

Sepiapterin reductase-0.562432199

81902298Brk1

Protein BRICK1-0.567691528

18202587Icam5

Intercellular adhesion molecule 5-0.573148962

41018346Synj1

Synaptojanin-1-0.595786704

81885934Tubb2a

Tubulin beta-2A chain-0.601153874

269849735Tle3

Transducin-like enhancer protein 3-0.61610973

61212993Appl1

DCC-interacting protein 13-alpha-0.620416865

73620902Frem1

FRAS1-related extracellular matrix protein 1-0.627906812

341940968Mpp3

MAGUK p55 subfamily member 3-0.638041447

48428638Slc6a17

Sodium-dependent neutral amino acid transporter SLC6A17-0.644043772

126986Prdx3

Thioredoxin-dependent peroxide reductase, mitochondrial-0.671887484

20177853Ctnnd2

Catenin delta-2-0.678544878

55976587Cops7a

COP9 signalosome complex subunit 7a-0.678759493

45476916Ipo5

Importin-5-0.679115063

187608891Psmb5

Proteasome subunit beta type-5-0.682690527

146345421Fscn1

Fascin-0.683093286

143341965Isoc2a

Isochorismatase domain-containing protein 2A, mitochondrial-0.688909422

341941177Nlgn3

Neuroligin-3-0.705928324

13637964Calb2

Calretinin-0.709645342

81906333Agk

Acylglycerol kinase, mitochondrial-0.724823504

294862528Aldh7a1

Alpha-aminoadipic semialdehyde dehydrogenase-0.739798228

62510641Qdpr

Dihydropteridine reductase-0.741245732

205371802Myh9

Myosin-9-0.759023756

8928227Ndrg3

Protein NDRG3-0.775680724

2495231Hint1

Histidine triad nucleotide-binding protein 1-0.794455526

21363036Trim2

Tripartite motif-containing protein 2-0.848578698

3023608Cyb5a

Cytochrome b5-0.858707624

25453098Cisd1

CDGSH iron-sulfur domain-containing protein 1-0.878522177

73920060Inpp4a

Type I inositol-3,4-bisphosphate 4-phosphatase-0.935804513

55583934Txlng

Gamma-taxilin-0.942858786

52782717Ppp2r2a

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform-0.954919786

76363295Phb2

Prohibitin-2-0.982419879

341942102Stxbp5

Syntaxin-binding protein 5-0.999837848

3219832Gpr143

G-protein coupled receptor 143-1.675131451

Table S12. Canonical signaling pathway analysis of proteins significantly and differentially regulated in T1R3KO mice hippocampus compared to WT control mouse hippocampus. For each significantly populated (p<0.05, n≥2 proteins per signaling pathway) the pathway description, the negative log10 transform of pathway population probability, the pathway enrichment ratio, percentage of downregulated or upregulated proteins populating the given signaling pathway are reported.

Canonical Signaling Pathway -log(p-value) Enrichment Ratio Downregulated Upregulated Cell Cycle Regulation by BTG Family Proteins 2.49E+00 1.00E-01 1/20 (5%) 1/20 (5%)Dopamine Receptor Signaling 2.89E+00 5.88E-02 2/51 (4%) 1/51 (2%)Wnt/β-catenin Signaling 3.26E+00 4.44E-02 2/90 (2%) 2/90 (2%)Mitotic Roles of Polo-Like Kinase 2.06E+00 6.06E-02 1/33 (3%) 1/33 (3%)Cyclins and Cell Cycle Regulation 1.82E+00 4.55E-02 1/44 (2%) 1/44 (2%)Cardiac β-adrenergic Signaling 2.26E+00 3.53E-02 1/85 (1%) 2/85 (2%)ILK Signaling 2.12E+00 3.16E-02 2/95 (2%) 1/95 (1%)Calcium Signaling 2.04E+00 2.94E-02 1/102 (1%) 2/102 (2%)Ceramide Signaling 1.64E+00 3.64E-02 1/55 (2%) 1/55 (2%)HIPPO signaling 1.57E+00 3.33E-02 1/60 (2%) 1/60 (2%)Telomerase Signaling 1.52E+00 3.12E-02 1/64 (2%) 1/64 (2%)CDK5 Signaling 1.47E+00 2.94E-02 1/68 (1%) 1/68 (1%)p70S6K Signaling 1.47E+00 2.94E-02 1/68 (1%) 1/68 (1%)PI3K/AKT Signaling 1.40E+00 2.70E-02 1/74 (1%) 1/74 (1%)Synaptic Long Term Depression 1.31E+00 2.41E-02 1/83 (1%) 1/83 (1%)

Table S13. BioFunction/Disease pathway analysis of proteins significantly and differentially regulated in T1R3KO mice hippocampus compared to WT control mouse hippocampus. For each significantly populated (p<0.05, n≥2 proteins per pathway) the BioFunction pathway description, the negative log10 transform of pathway population probability and the populating protein Gene Symbols are reported.

Diseases or Functions Annotation -log(p-value) Proteins

neuritogenesis 2.38E+00CTNND2,NLGN3,PPP2CA,RAP2A

dendritic growth/branching 1.87E+00 CTNND2,NLGN3,RAP2Aquantity of neurons 1.35E+00 NLGN3,RAP2A

Table S14. Proteins altered in expression between T1R3KO and WT cortical tissues. For each protein reliabily identified as being differentially regulated between T1R3KO and WT cortex the protein accession, Gene Symbol, Description and the log2 transform of the T1R3KO:WT iTRAQ ratio is displayed. Proteins demonstrating a difference of expression outside of the 95% confidence limits are highlighted in red (upregulated in T1R3KO compared to WT) or green (downregulated in T1R3KO compared to WT).

Protein Accession Gene Symbol Protein Description Log2 iTRAQ ratio83305086

Nrxn1Neurexin-1-alpha

1.626226549152032607

Sbf1Myotubularin-related protein 5

1.6228291041174621

Cct8T-complex protein 1 subunit theta

1.41841844551316522

Cers4Ceramide synthase 4

1.386286476187608891

Psmb5Proteasome subunit beta type-5

1.381218148341940723

GabpaGA-binding protein alpha chain

1.38051661318202672

NptxrNeuronal pentraxin receptor

1.369249415122065941

Rab12Ras-related protein Rab-12

1.28629123630315914

Ahsa1Activator of 90 kDa heat shock protein ATPase homolog 1

1.25702704578099089

Pip5k1cPhosphatidylinositol-4-phosphate 5-kinase type-1 gamma

1.237180188341941106

Fam169aProtein FAM169A

1.23553900244888326

SyncripHeterogeneous nuclear ribonucleoprotein Q

1.23372806130580482

Pcyt2Ethanolamine-phosphate cytidylyltransferase

1.1697228559910830

Psma4Proteasome subunit alpha type-4

1.151804091341941121

Gnl1Guanine nucleotide-binding protein-like 1

1.144079118416630

Aplp1Amyloid-like protein 1

1.12886503314916554

Dpy30Protein dpy-30 homolog

1.066464302146345507

Rpsa40S ribosomal protein SA

1.05007222150403739

Grin2aGlutamate [NMDA] receptor subunit epsilon-1

1.04683418413633865

RhoaTransforming protein RhoA

1.01089639981913122

Eif5a2Eukaryotic translation initiation factor 5A-2

1.008935146341940488

Eif3fEukaryotic translation initiation factor 3 subunit F

1.00589466581910090

Tom1l2TOM1-like protein 2

1.00427109847116230

GrhprGlyoxylate reductase/hydroxypyruvate reductase

0.96088467360390645

Cyb5r3NADH-cytochrome b5 reductase 3

0.95720166481900953

Coq9Ubiquinone biosynthesis protein COQ9, mitochondrial

0.94598016481898160

Dnm3Dynamin-3

0.907988065131884

NrasGTPase NRas

0.904437962166214952

MndaInterferon-activable protein 205-B

0.89982133138257365

Gdap1Ganglioside-induced differentiation-associated protein 1

0.8853280822495231

Hint1Histidine triad nucleotide-binding protein 1

0.873019484117066

Cox3Cytochrome c oxidase subunit 3

0.8717769637999666

Atl1Atlastin-1

0.867706368341941239

P4hbProtein disulfide-isomerase

0.8627237132363497

EzrEzrin

0.846424348122834

AladDelta-aminolevulinic acid dehydratase

0.829666868341942175

PtpraReceptor-type tyrosine-protein phosphatase alpha

0.810065808

1346247H1f0

Histone H1.00.794315271

81882902Arhgap1

Rho GTPase-activating protein 10.785501841

18266783Copb2

Coatomer subunit beta'0.728258707

47117303Eif1b

Eukaryotic translation initiation factor 1b0.725616756

38258292Rem1

GTP-binding protein REM 10.717370315

3914345Pip4k2a

Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha0.71252079

20138800Itsn1

Intersectin-10.710157522

52782717Ppp2r2a

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform 0.691809708

3914939Psap

Sulfated glycoprotein 10.672427166

13124192Eef1d

Elongation factor 1-delta0.649489085

73920250Vbp1

Prefoldin subunit 30.647436133

62900285Echs1

Enoyl-CoA hydratase, mitochondrial0.634455812

1709972Rpl10a

60S ribosomal protein L10a0.628638212

51338697Vsnl1

Visinin-like protein 10.61714349

54039806Ube2l3

Ubiquitin-conjugating enzyme E2 L30.59728252

45476974Ddah1

N(G),N(G)-dimethylarginine dimethylaminohydrolase 10.594500946

50401188Cnpy2

Protein canopy homolog 20.59350951

123787495 TMEM131L protein

Transmembrane protein 131-like0.588795176

68053169Xpo1

Exportin-10.587066566

61246343Snrpd3

Small nuclear ribonucleoprotein Sm D30.577554931

1346600Mtx1

Metaxin-10.569183907

51702252Hspd1

60 kDa heat shock protein, mitochondrial0.560069432

257051017Hagh

Hydroxyacylglutathione hydrolase, mitochondrial0.550304969

122066700Vapa

Vesicle-associated membrane protein-associated protein A0.546853911

160409932Sh3glb2

Endophilin-B20.539956669

81905699Ociad2

OCIA domain-containing protein 20.520729909

2500582Npm1

Nucleophosmin0.513664556

341940968Mpp3

MAGUK p55 subfamily member 30.510113964

22001911Rpl4

60S ribosomal protein L40.506876293

38372441Nrcam

Neuronal cell adhesion molecule0.500255809

125987842Myo18a

Myosin-XVIIIa0.48865828

81896015Samm50

Sorting and assembly machinery component 50 homolog0.488036696

341940599Maoa

Amine oxidase [flavin-containing] A0.484979929

46577689Rab21

Ras-related protein Rab-210.484735508

114151682Alkbh5

Probable alpha-ketoglutarate-dependent dioxygenase ABH50.483111717

47117682Cops2

COP9 signalosome complex subunit 20.475283157

61212993Appl1

DCC-interacting protein 13-alpha0.472693445

334302763 Ankdd1b Ankyrin repeat and death domain-containing protein 1B0.469336411

342187017Slc17a7

Vesicular glutamate transporter 10.461950019

341940603Anks1b

Ankyrin repeat and sterile alpha motif domain-containing protein 1B0.461377624

51702258Dnaja1

DnaJ homolog subfamily A member 10.459228687

341940436Ddx5

Probable ATP-dependent RNA helicase DDX50.455439639

24638218Sh3bgrl3

SH3 domain-binding glutamic acid-rich-like protein 30.453015417

341941270Plcb1

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-10.450036637

50400892Kctd12

BTB/POZ domain-containing protein KCTD120.449132782

81892818Ppp1r9b

Neurabin-20.437609927

81911483Camk2d

Calcium/calmodulin-dependent protein kinase type II subunit delta0.436307904

1709737Pitpna

Phosphatidylinositol transfer protein alpha isoform0.430331526

34395652 Rapgef2 Rap guanine nucleotide exchange factor 20.428761125

38604983Abhd12

Monoacylglycerol lipase ABHD120.404140643

54038801Ppp2ca

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform0.402531035

81913130Tiprl

TIP41-like protein0.402414935

38372626Arpc4

Actin-related protein 2/3 complex subunit 40.388881151

18314334Fkbp4

Peptidyl-prolyl cis-trans isomerase FKBP40.388584912

68775966Tubb6

Tubulin beta-6 chain0.376321211

8928249Ptges3

Prostaglandin E synthase 30.376150432

51702253Cplx1

Complexin-10.368642311

90110410Mpst

3-mercaptopyruvate sulfurtransferase0.368104225

81879688Pip4k2c

Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma0.363368098

55976720Trim46

Tripartite motif-containing protein 460.363327645

290457610Uba5

Ubiquitin-like modifier-activating enzyme 50.362667305

47117909Rpl15

60S ribosomal protein L150.360489316

81906657Noa1

Uncharacterized protein C4orf14 homolog0.357655879

55976655Ywhah

14-3-3 protein eta0.354428192

81906185Apoo

Apolipoprotein O0.349720535

341941138Lta4h

Leukotriene A-4 hydrolase0.348668284

338817897Pzp

Alpha-2-macroglobulin0.344546965

81903322Acsbg1

Long-chain-fatty-acid--CoA ligase ACSBG10.343840167

29428127Slc32a1

Vesicular inhibitory amino acid transporter0.3421687

28376967Cend1

Cell cycle exit and neuronal differentiation protein 10.339929732

97046551Agap2

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 20.339552924

48474741Sec22b

Vesicle-trafficking protein SEC22b0.333037897

341940817Idh1

Isocitrate dehydrogenase [NADP] cytoplasmic0.33046269

47117859Aqp4

Aquaporin-40.328141588

81886045Fsd1

Fibronectin type III and SPRY domain-containing protein 10.325444689

1352217Gad2

Glutamate decarboxylase 20.322925206

54039390Rps17

40S ribosomal protein S170.32260334

59797879Dlg2

Disks large homolog 20.321140509

341941223Pabpc1

Polyadenylate-binding protein 10.318770819

3023549Cox17

Cytochrome c oxidase copper chaperone0.318661136

81901922Sgip1

SH3-containing GRB2-like protein 3-interacting protein 10.3133065

146345480Pdia3

Protein disulfide-isomerase A30.313164397

341940743Ggt7

Gamma-glutamyltransferase 70.310407552

341941260Pik3cd

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform0.308107612

341940297Cab39

Calcium-binding protein 390.306891294

27151768Gad1

Glutamate decarboxylase 10.306581084

20177853Ctnnd2

Catenin delta-20.306189146

73919260Dync1li1

Cytoplasmic dynein 1 light intermediate chain 10.300887958

71153045Pgm2l1

Glucose 1,6-bisphosphate synthase0.300353827

108885300H2afv

Histone H2A.V0.295603403

231557Apoa1

Apolipoprotein A-I0.292550424

3915671Des

Desmin0.292402852

55583934Txlng

Gamma-taxilin0.292182067

67460420Cds2

Phosphatidate cytidylyltransferase 20.2899247

14916553Dnaja2

DnaJ homolog subfamily A member 20.288151208

341940408Cspg5

Chondroitin sulfate proteoglycan 50.28688602

341941109Eps15l1

Epidermal growth factor receptor substrate 15-like 10.285784633

110825706Arpc2

Actin-related protein 2/3 complex subunit 20.281590355

341940932Me1

NADP-dependent malic enzyme0.279189352

3219774Prdx6

Peroxiredoxin-60.275516256

29427667Sirpa

Tyrosine-protein phosphatase non-receptor type substrate 10.275419845

341940634Bsn

Protein bassoon0.27085166

1352173Cox6a1

Cytochrome c oxidase subunit 6A1, mitochondrial0.269975604

251757493Gnaq

Guanine nucleotide-binding protein G(q) subunit alpha0.269900461

81905494Fam162a

Protein FAM162A0.268356821

20978552Psmd6

26S proteasome non-ATPase regulatory subunit 60.266230949

23831273Ppp1r1b

Protein phosphatase 1 regulatory subunit 1B0.264145777

32363397Ndufb4

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 40.263635256

59797853Dlg1

Disks large homolog 10.259963153

266414Ckmt1

Creatine kinase U-type, mitochondrial0.258936

1729865Tcp1

T-complex protein 1 subunit alpha0.256350433

81882145Pitpnm1

Membrane-associated phosphatidylinositol transfer protein 10.255460762

29840839Pebp1

Phosphatidylethanolamine-binding protein 10.254097137

123786110 Kiaa1468 LisH domain and HEAT repeat-containing protein KIAA14680.252782361

146345407Ctbp1

C-terminal-binding protein 10.249916749

51338651Rps26

40S ribosomal protein S260.248804667

342187361Wdr7

WD repeat-containing protein 70.247445778

24211558Clic4

Chloride intracellular channel protein 40.246773564

41017503Nsfl1c

NSFL1 cofactor p470.245203742

22095581Dctn2

Dynactin subunit 20.244748135

81869786Tenm1

Teneurin-10.244634081

6225769Nmt1

Glycylpeptide N-tetradecanoyltransferase 10.244476451

81901722Snap47

Synaptosomal-associated protein 470.244421202

51316048Epm2aip1

EPM2A-interacting protein 10.244180689

146345423Gfap

Glial fibrillary acidic protein0.242489404

2829482Slc8a1

Sodium/calcium exchanger 10.241299604

129392Sub1

Activated RNA polymerase II transcriptional coactivator p150.240475576

32130449Rpl12

60S ribosomal protein L120.236578537

83305135Ndufs6

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial0.235813266

20141656Stx1a

Syntaxin-1A0.235713833

25453324Vps29

Vacuolar protein sorting-associated protein 290.235547358

2498751Pea15a

Astrocytic phosphoprotein PEA-150.231740985

45476916Ipo5

Importin-50.228762992

51701449Sdhb

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial0.227828431

81906333Agk

Acylglycerol kinase, mitochondrial0.227821507

52783213Serbp1

Plasminogen activator inhibitor 1 RNA-binding protein0.227424234

2493731CLTA

Clathrin light chain A0.21992096

1350769Rpl7

60S ribosomal protein L70.219239707

3913376Crym

Thiomorpholine-carboxylate dehydrogenase0.219129944

20141804Ppp5c

Serine/threonine-protein phosphatase 50.217038911

52783239Rpl14

60S ribosomal protein L140.216169482

51338605Rab3c

Ras-related protein Rab-3C0.213253402

20141319Gnaz

Guanine nucleotide-binding protein G(z) subunit alpha0.209251202

20139259Pin1

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 10.208657396

32469605Cap2

Adenylyl cyclase-associated protein 20.205296646

50402098Eef1a2

Elongation factor 1-alpha 20.204845538

121430Gria1

Glutamate receptor 10.203793506

2506252Pdcd6

Programmed cell death protein 60.202356643

38257686Gdap1l1

Ganglioside-induced differentiation-associated protein 1-like 10.195303658

123788967 Fam92b Protein FAM92B0.194337277

158518416Idh2

Isocitrate dehydrogenase [NADP], mitochondrial0.194076714

342187144Atp6v0a1

V-type proton ATPase 116 kDa subunit a isoform 10.193792545

549060Cct7

T-complex protein 1 subunit eta0.190592482

51704260Bsg

Basigin0.189980465

13959400Ak1

Adenylate kinase isoenzyme 10.189840881

266608Nme2

Nucleoside diphosphate kinase B0.189382024

14549168Grin2b

Glutamate [NMDA] receptor subunit epsilon-20.186636845

3929369 Srsf5 Serine/arginine-rich splicing factor 50.185644951

2501665Wnt10a

Protein Wnt-10a0.185338425

2829481Idh3g

Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial0.183298916

114978 Sept4

Septin-40.182962088

56404602Ablim2

Actin-binding LIM protein 20.182717191

341940296Casp8ap2

CASP8-associated protein 20.179881527

119348Eno2

Gamma-enolase0.17839815

1346597Atoh1

Protein atonal homolog 10.177868349

13431527Fibp

Acidic fibroblast growth factor intracellular-binding protein0.176390761

23396508Cndp2

Cytosolic non-specific dipeptidase0.176329636

62510502 Chchd6 Coiled-coil-helix-coiled-coil-helix domain-containing protein 60.175512111

9910790Tagln3

Transgelin-30.173106425

48428638Slc6a17

Sodium-dependent neutral amino acid transporter SLC6A170.171382588

56404944Park7

Protein DJ-10.171311777

81916660Cadm3

Cell adhesion molecule 30.169224724

341942004Slc6a11

Sodium- and chloride-dependent GABA transporter 30.168653831

46577639Rab10

Ras-related protein Rab-100.168378264

547881Gpm6a

Neuronal membrane glycoprotein M6-a0.168136323

41017420Lypla2

Acyl-protein thioesterase 20.167254668

71153505Dhx9

ATP-dependent RNA helicase A0.167035114

294862498Oxr1

Oxidation resistance protein 10.166400268

49037483Calm2

Calmodulin0.165533802

33112324Hspa12a

Heat shock 70 kDa protein 12A0.165362932

1351942Anxa5

Annexin A50.165067316

52000925Kcna2

Potassium voltage-gated channel subfamily A member 20.164709524

152031595Dmxl2

DmX-like protein 20.164434269

81886139Cep120

Centrosomal protein of 120 kDa0.163519798

417489Pcmt1

Protein-L-isoaspartate(D-aspartate) O-methyltransferase0.16211733

341940511Dctn1

Dynactin subunit 10.161169709

52783594Gng2

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-20.160727308

17368615Mlf2

Myeloid leukemia factor 20.159911043

14916536Bin1

Myc box-dependent-interacting protein 10.158973167

1705943Pde1b

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B0.158797366

341940560Cadps

Calcium-dependent secretion activator 10.158205123

52782785Sdha

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial0.154681049

54036519Syngr3

Synaptogyrin-30.152816975

341942101Stx16

Syntaxin-160.151767415

73917637Baiap2

Brain-specific angiogenesis inhibitor 1-associated protein 20.150519809

67460966Nono

Non-POU domain-containing octamer-binding protein0.150332851

2492687Snap91

Clathrin coat assembly protein AP1800.150222329

126986Prdx3

Thioredoxin-dependent peroxide reductase, mitochondrial0.149547652

20455200Ppp2r4

Serine/threonine-protein phosphatase 2A activator0.148444101

94730399Nefh

Neurofilament heavy polypeptide0.147540155

13633250Prep

Prolyl endopeptidase0.143419024

46397704Rps20

40S ribosomal protein S200.141670776

97051843Gprin1

G protein-regulated inducer of neurite outgrowth 10.141641744

38258249Nt5c

5'(3')-deoxyribonucleotidase, cytosolic type0.136828745

13638207Pfkm

6-phosphofructokinase, muscle type0.134940932

45477158Acsl6

Long-chain-fatty-acid--CoA ligase 60.131887598

115503759Aak1

AP2-associated protein kinase 10.130337811

52782789Fam49b

Protein FAM49B0.129688955

110816418Slirp

SRA stem-loop-interacting RNA-binding protein, mitochondrial0.129577524

135835Ptma

Prothymosin alpha0.129421977

12643614Atp2a2

Sarcoplasmic/endoplasmic reticulum calcium ATPase 20.128592429

1710815S100b

Protein S100-B0.127738912

85541765Blvrb

Flavin reductase (NADPH)0.123401451

51317305Gnb2

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-20.123102701

118572640Dld

Dihydrolipoyl dehydrogenase, mitochondrial0.122714396

55976751Atp1a1

Sodium/potassium-transporting ATPase subunit alpha-10.121128856

172044688Ehd3

EH domain-containing protein 30.121057171

120223Fkbp1a

Peptidyl-prolyl cis-trans isomerase FKBP1A0.120951587

13959548Rasa2

Ras GTPase-activating protein 20.120749959

145566796Myl12b

Myosin regulatory light chain 12B0.120445159

205830863Map6

Microtubule-associated protein 60.120210469

189045497Ccdc173

Uncharacterized protein C2orf77 homolog0.117056208

62510660Cyb5b

Cytochrome b5 type B0.116238944

47117101Rpl27

60S ribosomal protein L270.115412026

59799776Prkacb

cAMP-dependent protein kinase catalytic subunit beta0.114064535

21362640Glo1

Lactoylglutathione lyase0.113325433

20981714Syngr1

Synaptogyrin-10.113267678

205830666Ncam1

Neural cell adhesion molecule 10.111677823

1351911Akr1b3

Aldose reductase0.111581122

81888798Ermn

Ermin0.109774707

52782768C21orf33

ES1 protein homolog, mitochondrial0.109285552

126752Marcks

Myristoylated alanine-rich C-kinase substrate0.108510072

54041237Prkar2b

cAMP-dependent protein kinase type II-beta regulatory subunit0.108509977

51702142Ube2v2

Ubiquitin-conjugating enzyme E2 variant 20.107841186

50400458Cnksr2

Connector enhancer of kinase suppressor of ras 20.106473552

14916635Pygm

Glycogen phosphorylase, muscle form0.105659814

341942152Rimbp2

RIMS-binding protein 20.105048894

3334269Ncam2

Neural cell adhesion molecule 20.104972352

30316201Hnrnpa3

Heterogeneous nuclear ribonucleoprotein A30.102926252

51703309Rps3

40S ribosomal protein S30.102823868

48427970Abat

4-aminobutyrate aminotransferase, mitochondrial0.102704642

52000726 Kiaa0513 Uncharacterized protein KIAA05130.102392091

14916570G3bp2

Ras GTPase-activating protein-binding protein 20.101710806

341941969Rgs7

Regulator of G-protein signaling 70.100874947

41688568Slc9a3r1

Na(+)/H(+) exchange regulatory cofactor NHE-RF10.100394828

150438863Cadm1

Cell adhesion molecule 10.099287107

51317403Hpcal1

Hippocalcin-like protein 10.098877473

81902047Lgalsl

Galectin-related protein A0.098410157

81901560Cd177

CD177 antigen0.098387053

41018346Synj1

Synaptojanin-10.098292126

113607Aldoa

Fructose-bisphosphate aldolase A0.097607926

166897986Cox5a

Cytochrome c oxidase subunit 5A, mitochondrial0.096598329

41016844Amph

Amphiphysin0.096386526

108935937Tceal3

Transcription elongation factor A protein-like 30.095715691

52783422Slc38a3

Sodium-coupled neutral amino acid transporter 30.092864726

67460489Cand1

Cullin-associated NEDD8-dissociated protein 10.091626313

549057Cct4

T-complex protein 1 subunit delta0.091507688

117502Calr

Calreticulin0.09112116

134614Sod1

Superoxide dismutase [Cu-Zn]0.08990271

12230747Wdr1

WD repeat-containing protein 10.089798714

47117767Stx1b

Syntaxin-1B0.08881287

17378829Mbp

Myelin basic protein0.088042894

59798463Lin7a

Protein lin-7 homolog A0.088021142

3023548Cox7b

Cytochrome c oxidase subunit 7B, mitochondrial0.087645469

269849735Tle3

Transducin-like enhancer protein 30.085228712

51338653Rps28

40S ribosomal protein S280.084397959

10719868Add1

Alpha-adducin0.084304256

205371802Myh9

Myosin-90.084087165

341940933Map1b

Microtubule-associated protein 1B0.084074113

97537229Sptbn1

Spectrin beta chain, brain 10.084024124

341940314Casp4

Caspase-40.083606384

81865997Ofd1

Oral-facial-digital syndrome 1 protein homolog0.082781695

14917005 Hspa9 Stress-70 protein, mitochondrial0.082171537

48429104Hnrnpk

Heterogeneous nuclear ribonucleoprotein K0.081393838

125550Prkca

Protein kinase C alpha type0.079800144

23503090Ndufa4

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 40.079128531

37082114Pnpo

Pyridoxine-5'-phosphate oxidase0.078122857

3024764Usp5

Ubiquitin carboxyl-terminal hydrolase 50.077003324

51316976Ap2s1

AP-2 complex subunit sigma0.076891055

25089776Atp5h

ATP synthase subunit d, mitochondrial0.076858408

85681904Uso1

General vesicular transport factor p1150.075001067

20454881Dbn1

Drebrin0.074761286

59797974Arpc1a

Actin-related protein 2/3 complex subunit 1A0.073645197

20178035Pgam1

Phosphoglycerate mutase 10.073576711

55977481Tubb4b

Tubulin beta-2C chain0.073305325

223634791Ank2

Ankyrin-20.072828451

543921Canx

Calnexin0.072506637

2493662Hspe1

10 kDa heat shock protein, mitochondrial0.071172422

464506Pcx

Pyruvate carboxylase, mitochondrial0.070043871

341941972Arhgap5

Rho GTPase-activating protein 50.069997893

341941093Fh1

Fumarate hydratase, mitochondrial0.069263663

52783085Ppa2

Inorganic pyrophosphatase 2, mitochondrial0.06895283

25453239Ola1

Obg-like ATPase 10.068458366

13123946Actn4

Alpha-actinin-40.067246087

22002065Rpl5

60S ribosomal protein L50.066702666

147744591 Sept3

Neuronal-specific septin-30.066540549

88911344Itgad

Integrin alpha-D0.065438831

122066202Sptan1

Spectrin alpha chain, brain0.06522653

134047901Rtn3

Reticulon-30.0651468

341940992Mink1

Misshapen-like kinase 10.064248376

205830867Nfu1

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial0.064153911

54038800Ppp3ca

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform0.064086603

46577661Ube2n

Ubiquitin-conjugating enzyme E2 N0.063907145

78099814Atp6v1h

V-type proton ATPase subunit H0.063844179

52783747Hmgb1

High mobility group protein B10.06357124

46577316Ube2d1

Ubiquitin-conjugating enzyme E2 D10.063442972

166223488Stox2

Storkhead-box protein 20.063385785

20138079Epb4.1l3

Band 4.1-like protein 30.063230373

59798433Polk

DNA polymerase kappa0.061964466

12585446Atp6v1d

V-type proton ATPase subunit D0.061703272

353526354Tpi1

Triosephosphate isomerase0.061510914

399310Ctnnb1

Catenin beta-10.060331979

298286902Nptn

Neuroplastin0.059926246

81867523Lrp1

Prolow-density lipoprotein receptor-related protein 10.059895716

114041Apoe

Apolipoprotein E0.059802066

52001083Pcp4

Purkinje cell protein 40.059520401

29427692Immt

Mitochondrial inner membrane protein0.059376482

146345463Ndufv2

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial0.058661077

18203410Uchl1

Ubiquitin carboxyl-terminal hydrolase isozyme L10.058432761

30913117Scrn1

Secernin-10.058363964

416677Atp5a1

ATP synthase subunit alpha, mitochondrial0.057805021

12643842Acot9

Acyl-coenzyme A thioesterase 9, mitochondrial0.057774317

47117239Scamp1

Secretory carrier-associated membrane protein 10.0570921

81895995Enoph1

Enolase-phosphatase E10.056068913

146291078Vcp

Transitional endoplasmic reticulum ATPase0.056030387

341941233Pccb

Propionyl-CoA carboxylase beta chain, mitochondrial0.055844525

73920803Syn2

Synapsin-20.054261595

123788828Olfm4

Olfactomedin-40.052744674

68565610Idh3a

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial0.052663829

2501457Usp17la

Ubiquitin carboxyl-terminal hydrolase DUB-10.052310297

166200058Rogdi

Protein rogdi homolog0.052027131

1730220Gng4

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-40.051629419

398990Dbi

Acyl-CoA-binding protein0.051338947

334305788Gnai1

Guanine nucleotide-binding protein G(i) subunit alpha-10.051083436

55977306Psmc6

26S protease regulatory subunit 10B0.050988437

129261Spp1

Osteopontin0.050949782

122028Hist1h2bm

Histone H2B type 1-M0.050351568

114393Atp1b1

Sodium/potassium-transporting ATPase subunit beta-10.048687185

41688724Mrps36

28S ribosomal protein S36, mitochondrial0.048538228

134035345Ube2z

Ubiquitin-conjugating enzyme E2 Z0.047394971

17380315Napa

Alpha-soluble NSF attachment protein0.047007098

46397725Snap25

Synaptosomal-associated protein 250.046972909

94730353Ina

Alpha-internexin0.046967894

146345422Gpi1

Glucose-6-phosphate isomerase0.045561306

182636954Slc12a5

Solute carrier family 12 member 50.044963751

6093647Pak1

Serine/threonine-protein kinase PAK 10.044301254

47117242Ndufs8

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial0.043693441

52783095Ppa1

Inorganic pyrophosphatase0.04333871

19862081Uqcrh

Cytochrome b-c1 complex subunit 6, mitochondrial0.043242714

81897852Pithd1

PITH domain-containing protein 10.043132004

46577330Hnrnph2

Heterogeneous nuclear ribonucleoprotein H20.043026793

341941034Kif5c

Kinesin heavy chain isoform 5C0.042796843

3122052Dpysl3

Dihydropyrimidinase-related protein 30.041099504

78099820Atp5d

ATP synthase subunit delta, mitochondrial0.040624084

47116573Actr3

Actin-related protein 30.040165607

5915871Calu

Calumenin0.039847255

46396175Nrgn

Neurogranin0.038994329

51338662Rpl19

60S ribosomal protein L190.037558164

37077864Auh

Methylglutaconyl-CoA hydratase, mitochondrial0.037291311

57012721Cs

Citrate synthase, mitochondrial0.037236573

118105Ppia

Peptidyl-prolyl cis-trans isomerase A0.03720576

1729962Tst

Thiosulfate sulfurtransferase0.036985396

47117658Arf3

ADP-ribosylation factor 30.036982014

54036496Tmsb10

Thymosin beta-100.036929229

81862370Cyfip2

Cytoplasmic FMR1-interacting protein 20.036493305

20454828Atp5f1

ATP synthase subunit b, mitochondrial0.035493616

3334470Mag

Myelin-associated glycoprotein0.034914492

42559420Pcbp1

Poly(rC)-binding protein 10.034498619

1709255Ncan

Neurocan core protein0.034479863

119362Hsp90b1

Endoplasmin0.033558133

341940631Arhgef2

Rho guanine nucleotide exchange factor 20.03219801

21263374Acly

ATP-citrate synthase0.031922907

21759079Cyc1

Cytochrome c1, heme protein, mitochondrial0.031501319

127982Nme1

Nucleoside diphosphate kinase A0.031341397

54038837Phb

Prohibitin0.031326725

3023608Cyb5a

Cytochrome b50.030123545

124028629Hnrnpa2b1

Heterogeneous nuclear ribonucleoproteins A2/B10.029478657

143955288Mcu

Calcium uniporter protein, mitochondrial0.029252238

94730394Camk2b

Calcium/calmodulin-dependent protein kinase type II subunit beta0.028390918

51701319Chd4

Chromodomain-helicase-DNA-binding protein 40.027787198

60390186Mapre3

Microtubule-associated protein RP/EB family member 30.027639301

68059559Cmas

N-acylneuraminate cytidylyltransferase0.027571762

116849Cfl1

Cofilin-10.023896153

20140777Uqcrq

Cytochrome b-c1 complex subunit 80.023651302

51317306Gnb5

Guanine nucleotide-binding protein subunit beta-50.023204183

66773801Cltc

Clathrin heavy chain 10.023127804

42559891Srcin1

SRC kinase signaling inhibitor 10.022710784

122065442Map1a

Microtubule-associated protein 1A0.021588893

2498308Reep5

Receptor expression-enhancing protein 50.021426431

549061Cct6a

T-complex protein 1 subunit zeta0.021318114

55977479Tuba1a

Tubulin alpha-1A chain0.021219639

17380333Uqcrb

Cytochrome b-c1 complex subunit 70.021146439

81903631Atp5g1

ATP synthase lipid-binding protein, mitochondrial0.020214598

32130423Acadl

Long-chain specific acyl-CoA dehydrogenase, mitochondrial0.019706176

417208Ckb

Creatine kinase B-type0.01940873

22653628Akr1a1

Alcohol dehydrogenase [NADP+]0.019389061

113334Ap2a1

AP-2 complex subunit alpha-10.018524806

22653923Palm

Paralemmin-10.018193972

52783229Psmd11

26S proteasome non-ATPase regulatory subunit 110.018103506

76364170Ddx1

ATP-dependent RNA helicase DDX10.017795624

121716Gstm1

Glutathione S-transferase Mu 10.017693528

399833Slc2a3

Solute carrier family 2, facilitated glucose transporter member 30.017332986

341940734Gucy1a3

Guanylate cyclase soluble subunit alpha-30.017302044

52000687Atp1a3

Sodium/potassium-transporting ATPase subunit alpha-30.016566518

166199463Hacd3

3-hydroxyacyl-CoA dehydratase 30.016446406

21759130Arhgdia

Rho GDP-dissociation inhibitor 10.016242857

20140091Sfxn3

Sideroflexin-30.015617885

46395721Actn1

Alpha-actinin-10.015444297

9910829Psma6

Proteasome subunit alpha type-60.015418548

8134844Slc30a3

Zinc transporter 30.015255235

146345384Ppp3r1

Calcineurin subunit B type 10.01460518

338817898Got1

Aspartate aminotransferase, cytoplasmic0.013829221

82592512Nckap1

Nck-associated protein 10.013107987

22256950Pacsin1

Protein kinase C and casein kinase substrate in neurons protein 10.012502159

51704193Vamp2

Vesicle-associated membrane protein 20.012333075

341941065Hsp90ab1

Heat shock protein HSP 90-beta0.012107671

48428722Ywhag

14-3-3 protein gamma0.010357754

112804Slc3a2

4F2 cell-surface antigen heavy chain0.010100323

81881914Sv2a

Synaptic vesicle glycoprotein 2A0.00966049

112984Got2

Aspartate aminotransferase, mitochondrial0.008807822

146345481Pgk1

Phosphoglycerate kinase 10.008026213

117104Cox5b

Cytochrome c oxidase subunit 5B, mitochondrial0.00794644

47605479Slc25a12

Calcium-binding mitochondrial carrier protein Aralar10.007671195

73620958Ddx3y

ATP-dependent RNA helicase DDX3Y0.006123276

73921210Ndfip2

NEDD4 family-interacting protein 20.00606406

117949769Pcsk1n

ProSAAS0.005636403

6093478Ndrg1

Protein NDRG10.005556889

38258618Sirt2

NAD-dependent deacetylase sirtuin-20.005198514

1708288Hprt

Hypoxanthine-guanine phosphoribosyltransferase0.004821835

52000959Gabrb3

Gamma-aminobutyric acid receptor subunit beta-30.004203529

20138857Fxyd1

Phospholemman0.004074215

341941247Bcan

Brevican core protein0.003635714

62900054Cntfr

Ciliary neurotrophic factor receptor subunit alpha0.003439434

54040727Fasn

Fatty acid synthase0.002975392

14548072Ide

Insulin-degrading enzyme0.002091725

66773992Atp1a2

Sodium/potassium-transporting ATPase subunit alpha-20.002070411

81901032Paf1

RNA polymerase II-associated factor 1 homolog0.000432772

54037693Prkcg

Protein kinase C gamma type-0.000753824

341940472Dync1h1

Cytoplasmic dynein 1 heavy chain 1-0.00078437

66773932Basp1

Brain acid soluble protein 1-0.000894073

117949796Tenm4

Teneurin-4-0.001196096

146345468Nefm

Neurofilament medium polypeptide-0.001570261

146345470Nsf

Vesicle-fusing ATPase-0.001864581

55977478Tuba4a

Tubulin alpha-4A chain-0.002380713

1174545Syt1

Synaptotagmin-1-0.003978808

124028616Lap3

Cytosol aminopeptidase-0.005033911

341941063Hk1

Hexokinase-1-0.005229517

341941236Pde1a

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A-0.005489831

90110042Osbpl1a

Oxysterol-binding protein-related protein 1-0.005636312

81893041Sdk2

Protein sidekick-2-0.006164619

77416862Tubgcp3

Gamma-tubulin complex component 3-0.00731429

12229953Psma5

Proteasome subunit alpha type-5-0.007475231

81910107Abr

Active breakpoint cluster region-related protein-0.008727006

6166129Cryab

Alpha-crystallin B chain-0.008920025

20522240Tpm1

Tropomyosin alpha-1 chain-0.009081572

21362536Dpysl5

Dihydropyrimidinase-related protein 5-0.010511566

47117166Ndufa12

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12-0.010566273

51701351Ap2b1

AP-2 complex subunit beta-0.011248926

6647866 Ube3a Ubiquitin-protein ligase E3A-0.011316712

341940935Map2

Microtubule-associated protein 2-0.011656067

20141407Slc1a3

Excitatory amino acid transporter 1-0.011706879

51316667Rps27

40S ribosomal protein S27-0.012149847

21759002Ndufab1

Acyl carrier protein, mitochondrial-0.01264993

1350914Rxrg

Retinoic acid receptor RXR-gamma-0.012890922

81916755Glod4

Glyoxalase domain-containing protein 4-0.01316596

2497507Dlg3

Disks large homolog 3-0.013877472

122441Hba-a1

Hemoglobin subunit alpha-0.014520344

5915682Alb

Serum albumin-0.01461533

54036698Actc1

Actin, alpha cardiac muscle 1-0.015059736

341940405Csnk2a1

Casein kinase II subunit alpha-0.015785894

215274769Rpl10l

60S ribosomal protein L10-like-0.016032477

61216668Rtn1

Reticulon-1-0.018156457

41019154Plp1

Myelin proteolipid protein-0.018208883

54039458Rps15a

40S ribosomal protein S15a-0.021036953

24212071Tomm22

Mitochondrial import receptor subunit TOM22 homolog-0.021813362

2498952Spr

Sepiapterin reductase-0.021961828

62510641Qdpr

Dihydropteridine reductase-0.022517106

81879780Sncb

Beta-synuclein-0.022670897

23396478Mpc2

Brain protein 44-0.022829424

117029COX2

Cytochrome c oxidase subunit 2-0.023189087

3023546Cox6b1

Cytochrome c oxidase subunit 6B1-0.023549809

14285350Atp2b2

Plasma membrane calcium-transporting ATPase 2-0.023695351

60391192Ywhae

14-3-3 protein epsilon-0.023925913

73621117Phf24

Protein KIAA1045-0.025108657

94730376Dpysl2

Dihydropyrimidinase-related protein 2-0.025216251

10720404Vdac1

Voltage-dependent anion-selective channel protein 1-0.026075552

68566157Ywhaq

14-3-3 protein theta-0.026084948

46396509Pdhb

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial-0.026362475

125199Prkar2a

cAMP-dependent protein kinase type II-alpha regulatory subunit-0.027097958

1169460Slc1a2

Excitatory amino acid transporter 2-0.027356332

22001904Rpl17

60S ribosomal protein L17-0.027475893

47117855Gdi1

Rab GDP dissociation inhibitor alpha-0.027794938

20141252Atp5j2

ATP synthase subunit f, mitochondrial-0.02797516

60390207Mapre2

Microtubule-associated protein RP/EB family member 2-0.028329327

51316977Ap2m1

AP-2 complex subunit mu-0.028696358

33516945Hnrnpd

Heterogeneous nuclear ribonucleoprotein D0-0.029539627

52001076Mapk1

Mitogen-activated protein kinase 1-0.029757829

341940931Me3

NADP-dependent malic enzyme, mitochondrial-0.029973037

13629369Pfkp

6-phosphofructokinase type C-0.031018927

60391212Aco2

Aconitate hydratase, mitochondrial-0.031139023

20455323Tubb3

Tubulin beta-3 chain-0.031469274

20140237Efhd2

EF-hand domain-containing protein D2-0.031540097

97536879Nefl

Neurofilament light polypeptide-0.03158758

32172431Dnm1

Dynamin-1-0.032015072

81885934Tubb2a

Tubulin beta-2A chain-0.032797439

52783597Gng3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3-0.032889638

47115849Actr1b

Beta-centractin-0.033085422

51702275Hspa8

Heat shock cognate 71 kDa protein-0.033256114

81906105Tprgl

Tumor protein p63-regulated gene 1-like protein-0.033812886

1346207Gstm5

Glutathione S-transferase Mu 5-0.033988199

81873664Sv2b

Synaptic vesicle glycoprotein 2B-0.03415446

21263432Atp5c1

ATP synthase subunit gamma, mitochondrial-0.034463312

62510940Letm1

LETM1 and EF-hand domain-containing protein 1, mitochondrial-0.034513798

81870005Ncdn

Neurochondrin-0.035279759

17380576Tspan7

Tetraspanin-7-0.035323586

2497313Mog

Myelin-oligodendrocyte glycoprotein-0.035417347

126048Ldha

L-lactate dehydrogenase A chain-0.03542728

41019466Syp

Synaptophysin-0.037662968

17380130Nipsnap1

Protein NipSnap homolog 1-0.038065965

18202836Ywhab

14-3-3 protein beta/alpha-0.038570454

145559539Atp6v1a

V-type proton ATPase catalytic subunit A-0.038628415

121747Gstp1

Glutathione S-transferase P 1-0.038673391

267190Uba1

Ubiquitin-like modifier-activating enzyme 1-0.038693845

30581069Sfxn5

Sideroflexin-5-0.039092902

28376965Acot7

Cytosolic acyl coenzyme A thioester hydrolase-0.039513487

2507330Eif4a2

Eukaryotic initiation factor 4A-II-0.039532478

51702779Pfn1

Profilin-1-0.039541981

341942150Rpl3

60S ribosomal protein L3-0.039833927

342187137Acaa2

3-ketoacyl-CoA thiolase, mitochondrial-0.04043008

549059Cct3

T-complex protein 1 subunit gamma-0.040921691

341942102Stxbp5

Syntaxin-binding protein 5-0.041147934

73620902Frem1

FRAS1-related extracellular matrix protein 1-0.041207555

118542Glud1

Glutamate dehydrogenase 1, mitochondrial-0.04205264

120975Gnao1

Guanine nucleotide-binding protein G(o) subunit alpha-0.04211588

12585517Atp6v1g2

V-type proton ATPase subunit G 2-0.0422941

68566057Sfpq

Splicing factor, proline- and glutamine-rich-0.04275303

81879424Hnrnpu

Heterogeneous nuclear ribonucleoprotein U-0.042828414

78103425Wasf1

Wiskott-Aldrich syndrome protein family member 1-0.042921564

254763263Coro2b

Coronin-2B-0.045031769

2506545Hspa5

78 kDa glucose-regulated protein-0.045909913

20455479Atp5b

ATP synthase subunit beta, mitochondrial-0.045962899

81906255Aif1l

Allograft inflammatory factor 1-like-0.046562609

1170384Hsp90aa1

Heat shock protein HSP 90-alpha-0.046850827

51317407Hpca

Neuron-specific calcium-binding protein hippocalcin-0.047313441

416827Cox4i1

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial-0.047630461

3334247C1qbp

Complement component 1 Q subcomponent-binding protein, mitochondrial-0.047816849

81885886Nap1l4

Nucleosome assembly protein 1-like 4-0.048051512

2829840Atp5j

ATP synthase-coupling factor 6, mitochondrial-0.049011217

46576875Rab8b

Ras-related protein Rab-8B-0.050084281

18202309Opa1

Dynamin-like 120 kDa protein, mitochondrial-0.050557383

109818808Dnajc6

Putative tyrosine-protein phosphatase auxilin-0.051077132

341940235Anxa7

Annexin A7-0.051560759

73920802Syn1

Synapsin-1-0.052598801

67460396Tufm

Elongation factor Tu, mitochondrial-0.053673782

57012952Pdhx

Pyruvate dehydrogenase protein X component, mitochondrial-0.054266534

146345383Car2

Carbonic anhydrase 2-0.054716708

1711560Stmn1

Stathmin-0.05510427

13432200Mapt

Microtubule-associated protein tau-0.055620263

341940371Cntnap1

Contactin-associated protein 1-0.056936934

120702Gapdh

Glyceraldehyde-3-phosphate dehydrogenase-0.056969345

341941159Ndufa9

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial -0.05785971

52783455Aldh5a1

Succinate-semialdehyde dehydrogenase, mitochondrial-0.058219644

81879524Smap1

Stromal membrane-associated protein 1-0.058787172

20137987Dclk1

Serine/threonine-protein kinase DCLK1-0.059026096

15214055Eif4h

Eukaryotic translation initiation factor 4H-0.059718439

54039312Rps13

40S ribosomal protein S13-0.059957781

160406731Sh3gl2

Endophilin-A1-0.060378102

47117625Atp6v1f

V-type proton ATPase subunit F-0.060956868

14548302Uqcrc2

Cytochrome b-c1 complex subunit 2, mitochondrial-0.061142753

3914098Impa1

Inositol monophosphatase 1-0.061779943

38372295Nfasc

Neurofascin-0.062209403

47117649Actr2

Actin-related protein 2-0.062930273

52788305Sucla2

Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial-0.063310684

47117291Ndufs5

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-0.063658013

32363386Ndufa10

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial -0.063970527

51316454Hpcal4

Hippocalcin-like protein 4-0.064273682

51315842Dbnl

Drebrin-like protein-0.064717363

2506794Kif1a

Kinesin-like protein KIF1A-0.064721756

3122030Crmp1

Dihydropyrimidinase-related protein 1-0.066044754

9789726 Sept7

Septin-7-0.066414082

47605546Atp5o

ATP synthase subunit O, mitochondrial-0.066636984

341942254Pgm2

Phosphoglucomutase-1-0.067607069

32470593Aldoc

Fructose-bisphosphate aldolase C-0.067792581

116918Cntn1

Contactin-1-0.067904114

341942067Cttn

Src substrate cortactin-0.068390379

81881592Arpc5l

Actin-related protein 2/3 complex subunit 5-like protein-0.068488519

68566306Dnm1l

Dynamin-1-like protein-0.068791005

22653814Mapk8

Mitogen-activated protein kinase 8-0.069541686

51702788Rac1

Ras-related C3 botulinum toxin substrate 1-0.069636153

56748875Dynll2

Dynein light chain 2, cytoplasmic-0.070027406

1345696Capza2

F-actin-capping protein subunit alpha-2-0.070656769

52001483Mapk3

Mitogen-activated protein kinase 3-0.071006205

146345417Etfa

Electron transfer flavoprotein subunit alpha, mitochondrial-0.072096087

341942137Slc26a5

Prestin-0.072136235

55977057Hist1h3h

Histone H3.1-0.073003621

62288910Rhpn2

Rhophilin-2-0.073820603

143811473Atp6v1e1

V-type proton ATPase subunit E 1-0.074865263

9978489Hapln1

Hyaluronan and proteoglycan link protein 1-0.075401693

81875980Asrgl1

L-asparaginase-0.075911606

1709998Rab2a

Ras-related protein Rab-2A-0.075934855

81881319Rufy3

Protein RUFY3-0.076154904

52000885Ywhaz

14-3-3 protein zeta/delta-0.076621357

52782731Ppp2r1a

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform -0.076769078

51338706Atp6v1b2

V-type proton ATPase subunit B, brain isoform-0.076837779

54036445Stip1

Stress-induced-phosphoprotein 1-0.077929382

1352004Atp1b2

Sodium/potassium-transporting ATPase subunit beta-2-0.077994309

57012946Tppp

Tubulin polymerization-promoting protein-0.078272644

254765198Tagap

T-cell activation Rho GTPase-activating protein-0.078553148

24418554Lamtor3

Ragulator complex protein LAMTOR3-0.078617446

341941131Pfkl

6-phosphofructokinase, liver type-0.078689106

341941780Uqcrc1

Cytochrome b-c1 complex subunit 1, mitochondrial-0.078700026

81873733Phyhipl

Phytanoyl-CoA hydroxylase-interacting protein-like-0.079937403

6707708Ak4

Adenylate kinase isoenzyme 4, mitochondrial-0.080028267

17432986 Epb41l2 Band 4.1-like protein 2-0.0803751

55976513Eif4b

Eukaryotic translation initiation factor 4B-0.081231875

117128Cox7c

Cytochrome c oxidase subunit 7C, mitochondrial-0.081329798

81884328Mars

Methionyl-tRNA synthetase, cytoplasmic-0.081826434

1170151Hist1h1e

Histone H1.4-0.082100373

77416861Gak

Cyclin-G-associated kinase-0.082417139

400275Map2k1

Dual specificity mitogen-activated protein kinase kinase 1-0.083135381

342187037Tnr

Tenascin-R-0.083945646

13432217Snca

Alpha-synuclein-0.084540579

342187059Tomm70a

Mitochondrial import receptor subunit TOM70-0.084636966

341940637Cap1

Adenylyl cyclase-associated protein 1-0.08498195

146345457Mdh2

Malate dehydrogenase, mitochondrial-0.08518248

20141353Gsta4

Glutathione S-transferase A4-0.08600761

122065897 Plec Plectin-0.0866929

126042Ldhb

L-lactate dehydrogenase B chain-0.086755998

23822162Snta1

Alpha-1-syntrophin-0.08692287

9910725Gda

Guanine deaminase-0.087400846

128101Gap43

Neuromodulin-0.087471

14916528Add2

Beta-adducin-0.087725651

52000832Acat1

Acetyl-CoA acetyltransferase, mitochondrial-0.088001801

32363398Ndufa6

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6-0.088332661

146345529Tubb4a

Tubulin beta-4 chain-0.089119478

50401563 Sept11

Septin-11-0.090946847

81915047Cadm4

Cell adhesion molecule 4-0.09165668

52783579Dynll1

Dynein light chain 1, cytoplasmic-0.09175292

51317340Hist1h4j

Histone H4-0.092663648

548409Pdha1

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial -0.093328858

48429206Stxbp1

Syntaxin-binding protein 1-0.093794679

54036535Skp1a

S-phase kinase-associated protein 1-0.094112195

13124470Pfn2

Profilin-2-0.094618365

62296680Rpl6

60S ribosomal protein L6-0.094774029

46396900Pygb

Glycogen phosphorylase, brain form-0.095401053

3914439Psmb4

Proteasome subunit beta type-4-0.09555196

18202285Eef2

Elongation factor 2-0.096071472

341940828Kpnb1

Importin subunit beta-1-0.096369216

20139162Ndufa13

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-0.097056785

67460582Gpr37l1

Endothelin B receptor-like protein 2-0.097616994

118573056Smek2

Serine/threonine-protein phosphatase 4 regulatory subunit 3B-0.099330412

48428679Oxct1

Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial-0.101357451

46577115Ndufb9

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9-0.102920903

13637776Eno1

Alpha-enolase-0.103074711

81882150Ap3d1

AP-3 complex subunit delta-1-0.104030356

302595876Ubb

Polyubiquitin-B-0.105642235

124056467Camk2a

Calcium/calmodulin-dependent protein kinase type II subunit alpha-0.105710487

21759257Slc25a3

Phosphate carrier protein, mitochondrial-0.107251246

47117840Napb

Beta-soluble NSF attachment protein-0.10796024

146345428Gpd2

Glycerol-3-phosphate dehydrogenase, mitochondrial-0.108373406

56754803Tubb5

Tubulin beta-5 chain-0.108397906

115558L1cam

Neural cell adhesion molecule L1-0.108616899

18202587 Icam5 Intercellular adhesion molecule 5-0.108756207

51338761Cnp

2',3'-cyclic-nucleotide 3'-phosphodiesterase-0.109109558

47606041Phyhip

Phytanoyl-CoA hydroxylase-interacting protein-0.111911442

47117301Ndufb7

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7-0.112273996

51702234Cycs

Cytochrome c, somatic-0.11311703

61215307 Ptprd Receptor-type tyrosine-protein phosphatase delta-0.113497519

81871880Arhgap44

Rho GTPase-activating protein 44-0.113710718

146345472Ogdh

2-oxoglutarate dehydrogenase, mitochondrial-0.114472482

38258917Rab5c

Ras-related protein Rab-5C-0.114576537

134047752Epb4.1l1

Band 4.1-like protein 1-0.114839175

730956Tkt

Transketolase-0.115363472

47117040Mgll

Monoglyceride lipase-0.115921272

20138411 Ptk2b Protein-tyrosine kinase 2-beta-0.11623829

17380314Napg

Gamma-soluble NSF attachment protein-0.117913673

92087001Mdh1

Malate dehydrogenase, cytoplasmic-0.117949607

97536358Hsph1

Heat shock protein 105 kDa-0.118831095

51316035Blzf1

Golgin-45-0.119386753

45476977Ipo7

Importin-7-0.119483105

46396655Set

Protein SET-0.119824779

18266844Sars

Seryl-tRNA synthetase, cytoplasmic-0.119953423

8928228Ndrg2

Protein NDRG2-0.12291506

146345448Pkm

Pyruvate kinase isozymes M1/M2-0.123457105

54037163Ganab

Neutral alpha-glucosidase AB-0.123746428

13626388Eef1g

Elongation factor 1-gamma-0.124606385

3287958Cpe

Carboxypeptidase E-0.126214743

341941800Rasal1

RasGAP-activating-like protein 1-0.126341885

81903585Slc25a46

Solute carrier family 25 member 46-0.126363983

81904702Serpinb1a

Leukocyte elastase inhibitor A-0.126413066

20141789Prdx5

Peroxiredoxin-5, mitochondrial-0.12672373

46397334Actb

Actin, cytoplasmic 1-0.128455759

121557Gpd1

Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic-0.128635519

116243094Ckap4

Cytoskeleton-associated protein 4-0.129593761

6685313Cldn11

Claudin-11-0.130266695

92090596Etfb

Electron transfer flavoprotein subunit beta-0.130550525

341941160Ndufs1

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial-0.130906871

84029467Slc4a4

Electrogenic sodium bicarbonate cotransporter 1-0.130948963

46397808Coro1a

Coronin-1A-0.131421821

2498425Grb2

Growth factor receptor-bound protein 2-0.132129541

47117296Ndufc2

NADH dehydrogenase [ubiquinone] 1 subunit C2-0.132173085

51338599Ran

GTP-binding nuclear protein Ran-0.132186742

119370262Anp32-ps

Putative acidic leucine-rich nuclear phosphoprotein 32 family member C-0.132264984

147644461Gpd1l

Glycerol-3-phosphate dehydrogenase 1-like protein-0.132575002

51317303Gnb1

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1-0.132687076

50401108Mrgpra2b

Mas-related G-protein coupled receptor member A2-0.132823388

547923Prdx1

Peroxiredoxin-1-0.132909725

30580818Ak3

GTP:AMP phosphotransferase, mitochondrial-0.13465187

1703188Slc25a5

ADP/ATP translocase 2-0.134862604

122513Hbb-b1

Hemoglobin subunit beta-1-0.136599453

38258239Rab3b

Ras-related protein Rab-3B-0.137586697

152031548Mllt4

Afadin-0.13806477

56405010Eef1a1

Elongation factor 1-alpha 1-0.13981873

146291096Atp6v1c1

V-type proton ATPase subunit C 1-0.139900159

81894445Smap2

Stromal membrane-associated protein 2-0.140064591

32363403Ndufb10

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-0.140329162

22653715Dpp6

Dipeptidyl aminopeptidase-like protein 6-0.142993215

90110719Igsf8

Immunoglobulin superfamily member 8-0.14349165

341941040Kbtbd11

Kelch repeat and BTB domain-containing protein 11-0.144335109

322510016Cdc42

Cell division control protein 42 homolog-0.14469077

47117273Ndufs2

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial-0.14587167

146345421Fscn1

Fascin-0.146232885

341940212Rnpep

Aminopeptidase B-0.147515759

81903636Fam136a

Protein FAM136A-0.147631125

20178336Tpm3

Tropomyosin alpha-3 chain-0.147811989

20138335Gng12

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12-0.149423101

25453322Vps35

Vacuolar protein sorting-associated protein 35-0.150845609

145559476Glul

Glutamine synthetase-0.151465313

150438864Cadm2

Cell adhesion molecule 2-0.151768376

76363295Phb2

Prohibitin-2-0.152580417

145559451Cbr1

Carbonyl reductase [NADPH] 1-0.153313794

31077176Hspa4l

Heat shock 70 kDa protein 4L-0.153848802

138536Vim

Vimentin-0.15406056

62511116Rap2a

Ras-related protein Rap-2a-0.154651527

81878911H2afj

Histone H2A.J-0.154920959

32363396Ndufa2

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2-0.156656304

66773987Krt77

Keratin, type II cytoskeletal 1b-0.158274213

116248181Fam131b

Protein FAM131B-0.158365407

44888264Otub1

Ubiquitin thioesterase OTUB1-0.158645393

223634732Suclg1

Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial-0.160913134

8928560Cox7a2

Cytochrome c oxidase subunit 7A2, mitochondrial-0.160951251

1172773Pura

Transcriptional activator protein Pur-alpha-0.161499544

41017053Clint1

Clathrin interactor 1-0.161951439

39931169Bphl

Valacyclovir hydrolase-0.16315295

32363402Ndufa8

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-0.163352329

47117298Ndufb5

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial-0.165342811

52783073Lsamp

Limbic system-associated membrane protein-0.16591771

122063307Soga3

Uncharacterized protein C6orf174 homolog-0.166455347

2495342Hspa4

Heat shock 70 kDa protein 4-0.167153504

21759077Cotl1

Coactosin-like protein-0.168501163

341942263Prps1

Ribose-phosphate pyrophosphokinase 1-0.169821614

9910832Psmb2

Proteasome subunit beta type-2-0.170618861

300669660Npepps

Puromycin-sensitive aminopeptidase-0.171745121

52782750Atp5l

ATP synthase subunit g, mitochondrial-0.171928241

81896262Iars2

Isoleucyl-tRNA synthetase, mitochondrial-0.17289586

55976533Matr3

Matrin-3-0.174917897

1352528 Notch1 Neurogenic locus notch homolog protein 1-0.176580891

46395932Gprc5b

G-protein coupled receptor family C group 5 member B-0.17658323

22654291Cct2

T-complex protein 1 subunit beta-0.176803831

81881713Mcts1

Malignant T cell-amplified sequence 1-0.177522963

50403629Rps4x

40S ribosomal protein S4, X isoform-0.177640178

18203409Dstn

Destrin-0.177858975

3041732Sod2

Superoxide dismutase [Mn], mitochondrial-0.178488279

83305642 Sept5

Septin-5-0.178794684

17380131Gbas

Protein NipSnap homolog 2-0.179595536

48428644Synpo

Synaptopodin-0.181100862

115311320Camkv

CaM kinase-like vesicle-associated protein-0.182456194

47116926Lancl2

LanC-like protein 2-0.186747455

51316996Cplx2

Complexin-2-0.188987613

51315949Fbxo2

F-box only protein 2-0.189037545

21431839Rph3a

Rabphilin-3A-0.189534867

3024089Lasp1

LIM and SH3 domain protein 1-0.190226353

94730421Rtn4

Reticulon-4-0.194293195

46576641Cacybp

Calcyclin-binding protein-0.194948442

341942109Spg11

Spatacsin-0.195332228

13637964Calb2

Calretinin-0.196367831

341941148Gnai2

Guanine nucleotide-binding protein G(i) subunit alpha-2-0.19846329

81881321Ttyh1

Protein tweety homolog 1-0.198759703

62287163Necap1

Adaptin ear-binding coat-associated protein 1-0.202646618

146345462Ndufs3

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial-0.203093512

18203578Ehd1

EH domain-containing protein 1-0.203242418

118572643Epn1

Epsin-1-0.203894377

62510833 Dlst Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate -0.204435632

dehydrogenase complex, mitochondrial341941110

Ep400E1A-binding protein p400

-0.20467788847117288

Rap1bRas-related protein Rap-1b

-0.207187299146291087

Tmod2Tropomodulin-2

-0.20730511630316333

Gpm6bNeuronal membrane glycoprotein M6-b

-0.20733792450401075

ItpkaInositol-trisphosphate 3-kinase A

-0.20795195921903382

Slc25a4ADP/ATP translocase 1

-0.208876719135831

Thy1Thy-1 membrane glycoprotein

-0.20951111749065818

Psmc126S protease regulatory subunit 4

-0.21108345832469783

Lgi1Leucine-rich glioma-inactivated protein 1

-0.21203963681898294

Vps26bVacuolar protein sorting-associated protein 26B

-0.212389291132626

Rrm2Ribonucleoside-diphosphate reductase subunit M2

-0.21316953281893945

Nadsyn1Glutamine-dependent NAD(+) synthetase

-0.21332887847606029

Pip4k2bPhosphatidylinositol-5-phosphate 4-kinase type-2 beta

-0.214082988146345477

Pafah1b2Platelet-activating factor acetylhydrolase IB subunit beta

-0.2157672321350822

Hnrnpa1Heterogeneous nuclear ribonucleoprotein A1

-0.21690908720138723

Slc25a11Mitochondrial 2-oxoglutarate/malate carrier protein

-0.21706789761217662

Ddx39bSpliceosome RNA helicase Ddx39b

-0.21806119550400980

Lrrc7Leucine-rich repeat-containing protein 7

-0.2228066555976587

Cops7aCOP9 signalosome complex subunit 7a

-0.22508218341940231

Ap2a2AP-2 complex subunit alpha-2

-0.22600313547117308

Ndufb8NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

-0.23173379671152969

Myh10Myosin-10

-0.2360619724418394

Aldh1l1Cytosolic 10-formyltetrahydrofolate dehydrogenase

-0.23627722861229841

PdxkPyridoxal kinase

-0.23753338210720281

Snx12Sorting nexin-12

-0.238624502341940983

Myo5aMyosin-Va

-0.23937320813626886

Gdi2Rab GDP dissociation inhibitor beta

-0.240224288147721201

Ppm1eProtein phosphatase 1E

-0.240430566341940891

Lamb3Laminin subunit beta-3

-0.243058948341941732

Psmc326S protease regulatory subunit 6A

-0.24392994562286597

Diras2GTP-binding protein Di-Ras2

-0.24600394521363012

TrfSerotransferrin

-0.246450085462064

Fabp5Fatty acid-binding protein, epidermal

-0.24782834481902092

Agap3Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

-0.24925496651702762

Pafah1b1Platelet-activating factor acetylhydrolase IB subunit alpha

-0.24987730234222626

Slc25a22Mitochondrial glutamate carrier 1

-0.25028686362510439

CltbClathrin light chain B

-0.251278094145559463

CaskPeripheral plasma membrane protein CASK

-0.25150350152000877

Uqcrfs1Cytochrome b-c1 complex subunit Rieske, mitochondrial

-0.25357385254039315

Rps1040S ribosomal protein S10

-0.25859190549065658

Timm10Mitochondrial import inner membrane translocase subunit Tim10

-0.260775779548378

Grin1Glutamate [NMDA] receptor subunit zeta-1

-0.263024282120982

Gna11Guanine nucleotide-binding protein subunit alpha-11

-0.263766857342187320

Wdr47WD repeat-containing protein 47

-0.26488104746397828

Ranbp1Ran-specific GTPase-activating protein

-0.26671001181901517

SdslSerine dehydratase-like

-0.268886618

81886983Thumpd3

THUMP domain-containing protein 3-0.270473351

2499837Psmb1

Proteasome subunit beta type-1-0.271011099

25453098Cisd1

CDGSH iron-sulfur domain-containing protein 1-0.279246853

30913410Ubxn6

UBX domain-containing protein 6-0.280210056

81878370Arhgap18

Rho GTPase-activating protein 18-0.280888189

46397834Rab7

Ras-related protein Rab-7a-0.288168636

338817851Add3

Gamma-adducin-0.28966494

56749655 Sept9

Septin-9-0.296865644

10719959Cpne6

Copine-6-0.301160783

46577103Rab14

Ras-related protein Rab-14-0.304198832

8928227Ndrg3

Protein NDRG3-0.310473386

146345497Atic

Bifunctional purine biosynthesis protein PURH-0.31195788

54039385Rps18

40S ribosomal protein S18-0.31197858

45477305 Sept8

Septin-8-0.312439895

81881843Aldh6a1

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial-0.315642615

2498700Omg

Oligodendrocyte-myelin glycoprotein-0.315846285

341940825Ikzf3

Zinc finger protein Aiolos-0.321791003

26006698Acadsb

Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial-0.322263341

66773929Rcn2

Reticulocalbin-2-0.323550053

27734429Mpp2

MAGUK p55 subfamily member 2-0.32598196

341940423Dync1i1

Cytoplasmic dynein 1 intermediate chain 1-0.330132295

78099781Itpr3

Inositol 1,4,5-trisphosphate receptor type 3-0.332107712

51702798Rab3a

Ras-related protein Rab-3A-0.33214035

8134322 Atp8a1 Probable phospholipid-transporting ATPase IA-0.335523767

1345668Capzb

F-actin-capping protein subunit beta-0.34081597

47115588Lancl1

LanC-like protein 1-0.345798654

47117844Slc6a1

Sodium- and chloride-dependent GABA transporter 1-0.347264879

21362402Sdhc

Succinate dehydrogenase cytochrome b560 subunit, mitochondrial-0.347393721

110279022Iqsec1

IQ motif and SEC7 domain-containing protein 1-0.351756506

81894883Ahcyl1

Putative adenosylhomocysteinase 2-0.354341716

21363036Trim2

Tripartite motif-containing protein 2-0.354694249

341942049Snx6

Sorting nexin-6-0.355345186

341940485Ecm1

Extracellular matrix protein 1-0.355909497

2499458Nptx1

Neuronal pentraxin-1-0.36687398

47605664Hapln4

Hyaluronan and proteoglycan link protein 4-0.368055309

47117306Rps19

40S ribosomal protein S19-0.36998421

20178348 Sept6

Septin-6-0.390923272

341941177Nlgn3

Neuroligin-3-0.391496457

257051075Trio

Triple functional domain protein-0.397800485

32699478Nvl

Nuclear valosin-containing protein-like-0.398857422

81902298Brk1

Protein BRICK1-0.407984714

3122044Dpysl4

Dihydropyrimidinase-related protein 4-0.409900879

47606442Myl6

Myosin light polypeptide 6-0.412572539

150421540Dnajb6

DnaJ homolog subfamily B member 6-0.420115108

54037523Csnk2b

Casein kinase II subunit beta-0.420998047

156630926Hepacam

Hepatocyte cell adhesion molecule-0.422815464

51701335Ctdsp2

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2-0.441436963

3024586Slc1a4

Neutral amino acid transporter A-0.44245347

34098594Eppk1

Epiplakin-0.458740371

67461050Gpr158

Probable G-protein coupled receptor 158-0.46828523

294862528Aldh7a1

Alpha-aminoadipic semialdehyde dehydrogenase-0.471374789

46396281Kcnma1

Calcium-activated potassium channel subunit alpha-1-0.477702868

27923753Cgn

Cingulin-0.48051489

3219832Gpr143

G-protein coupled receptor 143-0.502636946

81886758Gucy1b3

Guanylate cyclase soluble subunit beta-1-0.521154764

94730407Pclo

Protein piccolo-0.535359677

46576352Cacna2d1

Voltage-dependent calcium channel subunit alpha-2/delta-1-0.587241948

1346910Adssl1

Adenylosuccinate synthetase isozyme 1-0.593517907

54039310Rps11

40S ribosomal protein S11-0.613996614

68565939Nudt3

Diphosphoinositol polyphosphate phosphohydrolase 1-0.639043955

146325019Ogt

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit -0.650875526

44888293Pdxp

Pyridoxal phosphate phosphatase-0.740961259

60392581Kcnab2

Voltage-gated potassium channel subunit beta-2-0.746651928

81895966Actbl2

Beta-actin-like protein 2-0.746787953

143341965Isoc2a

Isochorismatase domain-containing protein 2A, mitochondrial-0.830766988

57015413Nckipsd

NCK-interacting protein with SH3 domain-0.923547803

Table S15. Canonical signaling pathway analysis of proteins significantly and differentially regulated in T1R3KO mice cortex compared to WT control mouse cortex. For each significantly populated (p<0.05, n≥2 proteins per signaling pathway) the pathway description, the negative log10 transform of pathway population probability, the pathway enrichment ratio, percentage of downregulated or upregulated proteins populating the given signaling pathway are reported.

Canonical Signaling Pathway -log(p-value) Enrichment Ratio Downregulated Upregulated Actin Cytoskeleton Signaling 3.61E+00 4.95E-02 0/101 (0%) 5/101 (5%)mTOR Signaling 3.48E+00 4.63E-02 0/108 (0%) 5/108 (5%)RhoA Signaling 3.38E+00 6.35E-02 0/63 (0%) 4/63 (6%)Rac Signaling 3.35E+00 6.25E-02 0/64 (0%) 4/64 (6%)Regulation of eIF4 and p70S6K Signaling 2.96E+00 4.94E-02 0/81 (0%) 4/81 (5%)Regulation of Actin-based Motility by Rho 2.66E+00 6.52E-02 0/46 (0%) 3/46 (7%)RhoGDI Signaling 2.64E+00 4.04E-02 0/99 (0%) 4/99 (4%)Ephrin Receptor Signaling 2.47E+00 3.64E-02 0/110 (0%) 4/110 (4%)Role of Tissue Factor in Cancer 2.33E+00 5.00E-02 1/60 (2%) 2/60 (3%)Signaling by Rho Family GTPases 2.29E+00 3.23E-02 0/124 (0%) 4/124 (3%)Regulation of Cellular Mechanics by Calpain Protease 1.93E+00 6.90E-02 0/29 (0%) 2/29 (7%)Glioma Invasiveness Signaling 1.88E+00 6.45E-02 0/31 (0%) 2/31 (6%)Actin Nucleation by ARP-WASP Complex 1.85E+00 6.25E-02 0/32 (0%) 2/32 (6%)EIF2 Signaling 1.83E+00 3.30E-02 0/91 (0%) 3/91 (3%)Chemokine Signaling 1.69E+00 5.13E-02 0/39 (0%) 2/39 (5%)Macropinocytosis Signaling 1.69E+00 5.13E-02 0/39 (0%) 2/39 (5%)PEDF Signaling 1.61E+00 4.65E-02 0/43 (0%) 2/43 (5%)Caveolar-mediated Endocytosis Signaling 1.61E+00 4.65E-02 0/43 (0%) 2/43 (5%)Breast Cancer Regulation by Stathmin1 1.59E+00 2.65E-02 0/113 (0%) 3/113 (3%)Agrin Interactions at Neuromuscular Junction 1.57E+00 4.44E-02 0/45 (0%) 2/45 (4%)Virus Entry via Endocytic Pathways 1.46E+00 3.85E-02 0/52 (0%) 2/52 (4%)Ceramide Signaling 1.44E+00 3.77E-02 0/53 (0%) 2/53 (4%)Ephrin B Signaling 1.44E+00 3.77E-02 0/53 (0%) 2/53 (4%)HMGB1 Signaling 1.37E+00 3.45E-02 0/58 (0%) 2/58 (3%)NGF Signaling 1.37E+00 3.45E-02 0/58 (0%) 2/58 (3%)Telomerase Signaling 1.36E+00 3.39E-02 0/59 (0%) 2/59 (3%)Cholecystokinin/Gastrin-mediated Signaling 1.36E+00 3.39E-02 0/59 (0%) 2/59 (3%)Gα12/13 Signaling 1.33E+00 3.28E-02 0/61 (0%) 2/61 (3%)CDK5 Signaling 1.31E+00 3.17E-02 0/63 (0%) 2/63 (3%)CCR3 Signaling in Eosinophils 1.31E+00 3.17E-02 0/63 (0%) 2/63 (3%)

Table S16. BioFunction/Disease pathway analysis of proteins significantly and differentially regulated in T1R3KO mice cortex compared to WT control mouse cortex. For each significantly populated (p<0.05, n≥2 proteins per pathway) the BioFunction pathway description, the negative log10 transform of pathway population probability and the populating protein Gene Symbols are reported.

Diseases or Functions Annotation -log(p-value) Proteinendocytosis of synaptic vesicles 3.33E+00 DNM3,PIP5K1Cdevelopment of hippocampal neurons 2.70E+00 EZR,RHOAlength of dendritic spines 2.56E+00 ITSN1,RHOAmorphology of neurons 2.09E+00 PTPRA,RHOA,SYNCRIPbranching of axons 2.06E+00 EZR,RHOAoutgrowth of neurites 1.97E+00 EZR,NRXN1,RHOAmorphology of cerebral cortex cells 1.73E+00 PTPRA,SYNCRIPlong-term potentiation of hippocampal CA1 region 1.65E+00 GRIN2A,PTPRAmigration of neurons 1.46E+00 PTPRA,RHOA

Table S17. Textrous!-based protein clustering of significantly-regulated proteins from T1R3KO hippocampus versus WT control hippocampus. For each specific cluster of related proteins Neuronal, Synapse, Endocytosis, Neurotransmission, Disease, Cytoskeleton the Gene Symbol and Log2 transformed iTRAQ expression ratio (T1R3KO:WT) is reported. For each cluster also the mean and sum of the protein expression ratios in that cluster are given.

NeuronalGene Symbol Log2 ratioCadm3 1.060526336Pin1 0.832141687Vps29 0.649988186Scamp1 0.638303729Uso1 0.636255459Cct6a 0.607832677Slc6a17 -0.644043772Prdx3 -0.671887484Ipo5 -0.679115063Nlgn3 -0.705928324Calb2 -0.709645342Trim2 -0.848578698Cisd1 -0.878522177Inpp4a -0.935804513

mean -0.117748379sum -1.648477299

SynapseGene Symbol Log2 ratioCadm3 1.060526336Cnksr2 0.771420348Scamp1 0.638303729Slc6a17 -0.644043772Nlgn3 -0.705928324Calb2 -0.709645342Trim2 -0.848578698Inpp4a -0.935804513Stxbp5 -0.999837848

mean -0.263732009sum -2.373588084

EndocytosisGene Symbol Log2 ratioCadm3 1.060526336Uchl5 1.051728731

Fbxo2 0.900195816Rap2a 0.776670565Cnksr2 0.771420348Vps29 0.649988186Scamp1 0.638303729Uso1 0.636255459Cct6a 0.607832677Appl1 -0.620416865Psmb5 -0.682690527Nlgn3 -0.705928324Cisd1 -0.878522177Inpp4a -0.935804513Stxbp5 -0.999837848

mean 0.151314773sum 2.269721593

NeurotransmissionGene Symbol Log2 ratioCadm3 1.060526336Fbxo2 0.900195816Cnksr2 0.771420348Nlgn3 -0.705928324Calb2 -0.709645342Trim2 -0.848578698Inpp4a -0.935804513Stxbp5 -0.999837848

mean -0.183456528sum -1.467652225

DiseaseGene Symbol Log2 ratioCadm3 1.060526336Uchl5 1.051728731Slc8a1 1.039097993Ppp2ca 0.818459445Ablim2 0.778720389Vps29 0.649988186Uso1 0.636255459Cct6a 0.607832677Frem1 -0.627906812Fscn1 -0.683093286Nlgn3 -0.705928324Calb2 -0.709645342Agk -0.724823504

Myh9 -0.759023756Ndrg3 -0.775680724Trim2 -0.848578698Cisd1 -0.878522177Inpp4a -0.935804513Phb2 -0.982419879

mean -0.104674621sum -1.988817799

CytoskeletonGene Symbol Log2 ratioUchl5 1.051728731Ppp2ca 0.818459445Ablim2 0.778720389Rap2a 0.776670565Uso1 0.636255459Cct6a 0.607832677Ctnnd2 -0.678544878Fscn1 -0.683093286Agk -0.724823504Myh9 -0.759023756Ndrg3 -0.775680724

mean 0.095318283sum 1.048501118

Table S18. Textrous!-based protein clustering of significantly-regulated proteins from T1R3KO cortex versus WT control cortex. For each specific cluster of related proteins Cytoskeleton, Monomeric G protein the Gene Symbol and Log2 transformed iTRAQ expression ratio (T1R3KO:WT) is reported. For each cluster also the mean and sum of the protein expression ratios in that cluster are given.

CytoskeletonGene Symbol Log2 ratio

Rab121.28629123

6

Pip5k1c1.23718018

8

Rhoa1.01089639

9

Eif3f1.00589466

5

Tom1l21.00427109

8

Coq90.94598016

4

Dnm30.90798806

5

Atl10.86770636

8

Ezr0.84642434

8

Arhgap10.78550184

1

Copb20.72825870

7

Eif1b0.72561675

6

Rem10.71737031

5Pip4k2a 0.71252079

Itsn10.71015752

2

Adssl1-

0.59351791

Pdxp-

0.74096126Nckipsd -0.9235478

mean0.62411286

1

sum11.2340314

9

Monomeric G protein

Gene Symbol Log2 ratio

Psmb51.38121814

8

Rab121.28629123

6

Pip5k1c1.23718018

8

Rhoa1.01089639

9

Eif3f1.00589466

5

Tom1l21.00427109

8

Dnm30.90798806

5

Nras0.90443796

2

Ezr0.84642434

8

Arhgap10.78550184

1

Copb20.72825870

7

Eif1b0.72561675

6

Rem10.71737031

5Pip4k2a 0.71252079

Itsn10.71015752

2

Nudt3-

0.63904396

Ogt-

0.65087553Nckipsd -0.9235478

mean0.65280893

1

sum11.7505607

6

Table S19. Textrous!-based extracted noun-phrases from differential hippocampal T1R3KO versus WT control protein expression data. For each noun-phrases extracted using Textrous! the associated Cosine Similarity Score is given.

Noun-Phrase Cosine Similarity Scoreaberrant axonal branching 0.199313559aberrant axonal segregation 0.155644835aberrant endocytic processing 0.176874639aberrant endosomal compartments 0.088948211aberrant ipsilateral axonal tract 0.150593214abnormal actin bundle assembly 0.380562234abnormal actin clusters 0.389997315abnormal actin structures 0.383540415abnormal axonal crossing 0.146168739abnormal axonal guidance 0.16624712abnormal axonal projections 0.167250271abnormal clustering 0.155425495abnormal cortical activity 0.268747153abnormal cytoskeletal actin organization 0.38567374abnormal endocytic trafficking 0.164157967abnormal endocytosis 0.23598628abnormal fiber connections 0.230557095abnormal flat cortex 0.210642154abnormal gabaergic neurotransmission 0.160229645abnormal neuronal migration 0.291628062abnormal projection 0.146998526absolute macrocephaly 0.223966092accessory olfactory bulb 0.194629854acidic endosomal vesicles 0.170306573actin bundle formation 0.387273819actin component 0.388439868actin crosslinking activity 0.387336471actin cytoskeletal abnormalities 0.384158182actin cytoskeletal network 0.385821996actin cytoskeletal organization 0.385566917actin cytoskeletal regulation 0.392775488actin cytoskeletal reorganization 0.385124944actin cytoskeleton 0.394470508actin cytoskeleton 0.394470508actin cytoskeleton organization 0.396071054actin cytoskeleton organization 0.396071054actin cytoskeleton rearrangement 0.380566681actin cytoskeleton rearrangement 0.380566681actin dynamics 0.382435314actin dysfunction 0.384415763

actin expression 0.423886604actin fiber disassembly 0.40133861actin fibers 0.419013294actin filament 0.381032289actin filament bundles 0.385410885actin filament dynamics 0.380682032actin filament length 0.381096708actin filament network 0.381360044actin filament organization 0.382503705actin filament structures 0.380426267actin filaments 0.381087898actin filaments anchor 0.388414644actin fold 0.385267921actin interface 0.382654614actin isoforms 0.434813453actin kinetics 0.386974859actin microfilaments 0.383452792actin modeling 0.382808508actin module results 0.387799722actin molecule 0.423900383actin monomer 0.382461295actin motion 0.385162235actin myopathy 0.383710635actin network 0.38570066actin network disassembly 0.382980888actin networks 0.388099112actin organization 0.385548512actin polymerization studies 0.382545086actin reorganization 0.385229865actin sequestration 0.382223008actin staining 0.41850169actin stress fiber assembly 0.38931748actin stress fiber formation 0.394834798actin stress fibers 0.41250775actin structures 0.382977603actin thin filaments 0.387283738activated synapses 0.157854311activating immunologic synapse 0.097659094actomyosin cytoskeleton 0.386318624adaptive neural plasticity 0.081387705adrenal cortex 0.206640387adrenal cortex cells 0.206950681adrenal cortical cancer 0.306156574adrenal cortical cancers 0.3130291adult adrenal cortex 0.221217946adult cerebellar neurons 0.195596486

adult cerebellum 0.244331114adult cerebellum cdna library 0.114153477adult cerebral cortex 0.217674895adult cortical layers 0.268377581adult frontal cerebral cortex 0.190183299adult frontal cortex 0.182143119adult hippocampus 0.197718007adult hippocampus cdna libraries 0.112801437adult hippocampus cdna library 0.105988439adult human cortex 0.260728947adult human hippocampus 0.245299079adult motor neurons 0.191559864adult mouse cerebellum 0.129998533adult mouse hippocampus 0.128383899adult purkinje cells 0.206746008adult rat cerebellum 0.221632684adult rat cortex 0.208033255adult rat hippocampus 0.192863769adult synapses 0.211894779adult-onset cerebellar ataxia 0.215511101adult-onset cortical myoclonus 0.251175753adult-onset neurologic disorder 0.132950542adult-onset pyramidal symptoms 0.082771057advanced neurologic symptoms 0.189262819afferent synapses 0.185087352airway epithelial gabaergic system 0.180483408albumin endocytosis 0.232321153altered cortical processes 0.297641783altered electrophysiologic synaptic activity 0.203596522altered neuronal migration 0.304454969ampa currents 0.089105724ampa glutamate receptors 0.045727227ampa receptor-interacting protein 0.087369219ampa receptor-mediated synaptic transmission 0.127038063ampa receptors 0.045727227ampa stimulation 0.088894326ampa synaptic currents 0.137354408anterior cerebellum 0.122551609anterograde axonal transport 0.170101185antibody clustering 0.176271665apical cortical membrane 0.352046844apical proximal tubular endocytosis 0.227281668apical recycling endosomes 0.205021817apparent clustering 0.207591514artificial synapse formation assay 0.219434974associative synaptic plasticity 0.170909971

asymmetric cortical localization 0.366105298asymmetric synapses 0.244689013ataxic cerebral palsy accounts 0.151496262ataxic gait 0.319724294ataxic mouse mutants 0.151496262ataxic movements 0.325833025ataxic symptoms 0.084093377ataxic syndrome 0.103410085autism postmortem cortex samples 0.213022104autophagic vacuole formation 0.120416863autophagic vacuole fraction 0.070181291autosomal dominant neurologic condition 0.186682104autosomal dominant neurologic disorder 0.132650709avian motor neurons 0.184452041avian tensin 0.247451712axon projection 0.177986167axonal action potential conduction 0.168500852axonal anterograde transport 0.170101185axonal atrophy 0.142891022axonal bundles 0.202039309axonal cell adhesion molecules 0.260644583axonal crossing 0.152902292axonal cytoskeleton 0.339686148axonal cytoskeleton 0.339686148axonal defects 0.139937712axonal densities 0.165856502axonal diameters 0.163718068axonal distribution 0.18600983axonal edema 0.158504901axonal elongation 0.176123689axonal enlargement 0.170233437axonal extension 0.175343819axonal fasciculation 0.297821582axonal fasciculation 0.17751729axonal form 0.183923168axonal fragmentation 0.160430901axonal gpi-anchored protein 0.17044374axonal guidance 0.175933924axonal guidance events 0.164841769axonal guidance protein 0.149659723axonal injury 0.157507337axonal integrity 0.16744855axonal lengthening 0.155657869axonal loss 0.162662638axonal maintenance 0.148300917axonal marker 0.234464403

axonal membrane 0.30245733axonal membrane compartment 0.271825258axonal membranes 0.222491091axonal microtubule bundles 0.131152197axonal microtubules 0.144677143axonal migration 0.269063155axonal networks 0.181721346axonal neurofilament swelling 0.171307778axonal neuropathies 0.164094815axonal neuropathy 0.128261611axonal outgrowth 0.170080866axonal outgrowth inhibition 0.22663372axonal polyneuropathy 0.161098742axonal processes 0.187441608axonal projection sites 0.148870907axonal projection sites 0.148870907axonal projections 0.177889538axonal protection 0.163309675axonal regeneration 0.151362456axonal relative 0.170241928axonal retrograde transport 0.179577045axonal shortening 0.155175592axonal stimulation 0.161044777axonal surface glycoproteins 0.236604469axonal survival 0.220664759axonal swellings 0.167726823axonal targeting 0.293050356axonal targeting element 0.2170824axonal terminals 0.195120123axonal tracing experiments 0.176171812axonal tract formation 0.190870737axonal tracts 0.157628577axonal trafficking 0.182124353axonal translation 0.229520616axonal transport 0.158700101axonal transport mapping 0.16598854axonal transport studies 0.16411032axons senses 0.18157597basal synaptic strength 0.200831583basal synaptic transmission 0.185728304bidirectional synaptic plasticity 0.178946005bilateral cortical malformation 0.296773221binding pdz 0.021330321blocking nmda receptors 0.083166606bovine adrenal cortex capillary endothelial cells 0.245288411bovine brain cortex 0.218497402

bovine cerebellum 0.278709081brain cortex 0.198282769brain neuronal cell bodies 0.26702397brain plasticity 0.181164479brain purkinje cells 0.221857237brain synapses 0.211471532brain synaptic plasma membranes 0.201522182branched actin filaments 0.38170866branched actin network formation 0.385160331broad complex neurologic spectrum 0.234462879broad neurologic phenotypic spectrum 0.203155288brush border cytoskeleton 0.406750101cancer cell cytoskeleton 0.309928103canine cerebral cortex 0.24745388carboxy-terminal pdz ligand 0.030448461cardiac cytoskeleton 0.258977987cardiac purkinje fiber cells 0.26778954cell axons 0.245472759cell cortex 0.267064663cellular cortex 0.234447283cellular endocytosis machinery 0.212307073cellular plasticity 0.168415877cellular synapse protein 0.143436669center deletion mouse purkinje neurons 0.166412997central axons 0.170335257central excitatory synapses 0.129822287central pdz domain 0.1297374central postsynaptic hearing impairment 0.145029135central synapses 0.178422431cerebellar abnormalities 0.221115646cerebellar abnormality 0.241984215cerebellar anlage 0.22382967cerebellar architecture 0.261706383cerebellar ataxia 0.224919132cerebellar ataxia due 0.220221398cerebellar ataxia mapping 0.234352429cerebellar atrophy 0.216303605cerebellar cell death 0.236392848cerebellar coordination problems 0.244901984cerebellar cortex 0.258349621cerebellar cortex 0.258349621cerebellar cortex purkinje cells 0.260089542cerebellar cortex purkinje cells 0.260089542cerebellar cortex purkinje cells 0.260089542cerebellar cysts 0.243576201cerebellar dentate 0.232436864

cerebellar dysarthria 0.25265798cerebellar expression 0.262056916cerebellar external granular layer 0.280553401cerebellar external granular layer cells 0.212942995cerebellar granular cell layer 0.298056829cerebellar granular layer 0.276815978cerebellar granule cell cultures 0.286295265cerebellar granule cell lineage 0.230419111cerebellar granule cell neurons 0.249606039cerebellar granule cell neurons 0.249606039cerebellar granule cell precursors 0.269015403cerebellar granule neuron populations 0.224683308cerebellar granule neuron precursors 0.223199746cerebellar granule neurons 0.202265344cerebellar hemispheres 0.246613744cerebellar hypoplasia 0.209397132cerebellar internal granule layer 0.245946084cerebellar malformation 0.269001763cerebellar malformations 0.217738808cerebellar mossy fibers 0.326592918cerebellar mossy fibers 0.326592918cerebellar motor control 0.244203853cerebellar motor learning 0.27063032cerebellar neoplasia 0.208462167cerebellar neuronal cell layers 0.313141266cerebellar neuronal cell layers 0.313141266cerebellar neuronal number 0.259517307cerebellar neuronal number 0.259517307cerebellar neurons 0.192783781cerebellar origin 0.245148886cerebellar parallel fibers 0.325681994cerebellar physiology 0.249161042cerebellar purkinje cell 0.311715469cerebellar purkinje cell 0.311715469cerebellar purkinje cell layer 0.317786156cerebellar purkinje cell layer 0.317786156cerebellar purkinje cell loss 0.30211703cerebellar purkinje cell loss 0.30211703cerebellar purkinje cells 0.239190369cerebellar purkinje cells 0.239190369cerebellar purkinje neurons 0.222122193cerebellar purkinje neurons 0.222122193cerebellar purkinje neurons 0.222122193cerebellar sections 0.257631305cerebellar signs 0.250582989cerebellar tissue 0.256486751

cerebellum beginning 0.267887147cerebellum caudate nucleus 0.144848159cerebellum cdna library 0.089768013cerebellum granule layer 0.261609018cerebellum library 0.264154821cerebellum mrna 0.166170472cerebellum phage library 0.22097735cerebellum samples 0.256678606cerebellum tissue 0.254828566cerebral cortex 0.213038722cerebral cortex activity 0.212086145cerebral cortex defects 0.187760416cerebral cortex pyramidal cells 0.202939896cerebral cortex pyramidal cells 0.202939896cerebral cortex show 0.212351537cerebral cortex tissue 0.229015556cerebral cortical layers 0.26409127cerebral cortical neurons 0.208618982characteristic neurologic signs 0.225089916chemical connections 0.178037638chemical synapses 0.189989745chicken brain synaptic proteins 0.194616172chicken temporal retinal axons 0.072058042chicken tensin 0.247923523childhood-onset cerebellar ataxia 0.213887218cholinergic axons 0.108163cholinergic projection neurons 0.141457838cholinergic synapses 0.120798397cingulate cortex 0.188064782classic neurologic abnormalities 0.212370105classical endosomal markers 0.154965069clathrin endocytic machinery 0.184626299clathrin-associated endocytosis 0.228775197clathrin-coated synaptic vesicles 0.200505063clear clustering 0.187486452clustering algorithms 0.170235238cns axons 0.072764085cns synapse elimination 0.068056348cns synapses 0.095000526common n-terminal pdz domain 0.08732394common recycling endosomes 0.168718059competitive nmda antagonist 0.04751804complete neurologic correction 0.182164824complex neurologic phenotype 0.225698775complex neurologic picture 0.223981476concomitant early axonal involvement 0.1541287

congenital cerebellar ataxia 0.271754748congenital contractures 0.176788819congenital ovine neuronal ceroid lipofuscinosis 0.259579837conserved integral component 0.081640013constitutive endocytosis 0.212681398contacting axons 0.187435881contractile cytoskeleton 0.40495527control rat primary motor cortex 0.230660421cortex ipsilateral 0.195031526cortical actin 0.437379087cortical actin 0.437379087cortical actin assembly 0.429314458cortical actin assembly 0.429314458cortical actin cytoskeleton 0.442071727cortical actin cytoskeleton 0.442071727cortical actin cytoskeleton 0.442071727cortical actin cytoskeletons 0.438207677cortical actin cytoskeletons 0.438207677cortical actin filaments 0.429119869cortical actin filaments 0.429119869cortical actin formation 0.432595391cortical actin formation 0.432595391cortical actin skeleton 0.433424252cortical actin skeleton 0.433424252cortical actin structures 0.435587416cortical actin structures 0.435587416cortical actomyosin contraction 0.359842843cortical area 0.280353403cortical areas 0.282274203cortical axons 0.262774503cortical axons 0.262774503cortical basement membrane 0.390558235cortical blindness 0.281967615cortical capillaries 0.27888199cortical cataract formation 0.270683359cortical cataracts 0.267404488cortical cell layers 0.302095864cortical clusters 0.294503077cortical collecting duct 0.261147333cortical collecting ducts 0.258930124cortical columns 0.285347135cortical compartments 0.292999428cortical concentration 0.280789511cortical consolidation 0.269686031cortical cultured neurons 0.214907042cortical cytoskeleton 0.446447108

cortical cytoskeleton 0.446447108cortical disorganization 0.295417279cortical distal tubules 0.270181677cortical distribution 0.286927971cortical expression 0.283605955cortical f-actin 0.402662749cortical fibers 0.343890384cortical flow 0.302828509cortical gabaergic interneurons 0.212716949cortical gabaergic neurons 0.208862825cortical gabaergic neurons 0.208862825cortical gabaergic transmission 0.242062836cortical glutamatergic neurons 0.205660158cortical granule exocytosis 0.27958499cortical identity 0.272408152cortical inhibition 0.314339631cortical integration 0.278970255cortical laminae 0.268859812cortical layer 0.293391274cortical layers 0.282392442cortical lissencephaly 0.287093542cortical localization 0.330422514cortical malformation 0.297975257cortical map formation 0.257695594cortical mass 0.273389721cortical material 0.283278821cortical microfilaments 0.298498695cortical morphogenesis 0.26634257cortical motor neurons 0.225243747cortical nephrons 0.275757303cortical networks 0.292927777cortical neuron culture 0.261014683cortical neuron positioning 0.276222224cortical neuronal networks 0.273129901cortical neuronal networks 0.273129901cortical neurons 0.20641741cortical neuropil 0.286355734cortical opacities 0.28495091cortical organization 0.297069377cortical origin 0.277990859cortical plasticity 0.270039142cortical plasticity 0.270039142cortical plate expression 0.266262822cortical plate neurons 0.204648544cortical precursors 0.258096066cortical projection neurons 0.208468379

cortical projection neurons 0.208468379cortical pyramidal cells 0.219047932cortical pyramidal neurons 0.211898861cortical pyramidal neurons 0.211898861cortical region 0.277482997cortical steroids 0.300820869cortical structures 0.284285163cortical synapses 0.286322734cortical synapses 0.286322734cortical synchrony 0.279876779cortical thickness 0.282285582cortical tissue 0.277729002cortical tremor 0.274144971cortical tubular epithelium 0.285401383cortical tubules 0.285831046cortical volume 0.278450224corticospinal axons 0.19358118corticospinal tract axons 0.188420063corticostriatal synapses 0.174048732critical period plasticity 0.12295957crude synaptic vesicle fraction 0.185668341c-terminal cytosolic pdz domain 0.140542368c-terminal endosomal targeting motif 0.15438979c-terminal pdz domain 0.0989742c-terminal pdz ligand 0.031726423c-terminal pdz motif 0.133809623c-terminal pdz type ii binding sequence 0.029039186cultured cerebellar granule cells 0.207008121cultured cerebellar granule neurons 0.210660222cultured cerebellar granule neurons 0.210660222cultured cerebellar purkinje cells 0.24277936cultured cerebellar purkinje cells 0.24277936cultured cortical neurons 0.214907042cultured embryonic rat cortical neurons 0.218768577cultured embryonic rat hippocampal neurons 0.18896079cultured human neuronal cell lines 0.288104321cultured motor neurons 0.197794483cultured mouse cerebellar purkinje cells 0.195158082cultured mouse cortical neurons 0.186831494cultured mouse primary cortical neurons 0.19918454cultured olfactory bulb neurons 0.186851204cultured olfactory bulb neurons 0.186851204cultured primary cortical neurons 0.23106232cultured primary neurons 0.191076473cultured primary rat cortical neurons 0.241018849cultured primary rat hippocampal neurons 0.205270249

cultured purkinje cells 0.206007591cultured rat brain hippocampal neurons 0.193988519cultured rat cerebral cortical neurons 0.227364749cultured rat cortical neurons 0.229493714cultured rat hippocampal neurons 0.194058542cultured rat neurons 0.192241046cultured rat superior cervical neurons 0.198201849cultured rat sympathetic neurons 0.189371846cytoskeletal actin 0.38371683cytoskeletal actin bundle 0.387708256cytoskeletal actin organization 0.385566917cytoskeleton architecture 0.397368362cytoskeleton arrangement 0.39485198cytoskeleton linker protein 0.210461141cytoskeleton organization 0.393390419cytoskeleton proteins 0.246942892cytoskeleton regulation 0.368307431decreased gabaergic 0.245929984decreased neuronal 0.255353492decreased neurons 0.189579564decreased renal cortex expression 0.201251666deep cerebellar layer 0.275132595deep cerebellar nuclei 0.230661342deep cortex 0.241955848deep cortical layers 0.290764166defective apical endocytosis 0.224147407defective neuronal migration 0.288407203defective olfactory bulb morphogenesis 0.158525294defective spine synapse formation 0.241781834defective synapse formation 0.192916703defective synaptic plasticity 0.166418998defined synapses 0.171927019delayed endocytosis 0.186164291delayed neuronal migration 0.282269265dendritic spine plasticity 0.180999492dendritic spine synapses 0.205928294dendritic synapses 0.178071054depressing central synapses 0.179934451developing adrenal cortex 0.201381607developing cerebellar cortex 0.230495554developing cerebellar cortex 0.230495554developing cerebellum 0.196281964developing cerebral cortex 0.189479724developing cortex 0.185628732developing hippocampus 0.154548446developing human cortex 0.234844251

developing human neocortex 0.215670363developing mouse cerebellum 0.105583582developing murine hippocampus 0.123378392developing neocortex 0.157772136developing rat cerebellum 0.198250633developing somatosensory cortex 0.18509692differentiated motor neurons 0.193872675diffuse axonal staining 0.243767461diffuse neurologic abnormalities 0.232565162digital contractures 0.205487249direct connections 0.177059803discrete cortical areas 0.27051677disease connections 0.183189682disorganized actin cytoskeleton 0.397281539disorganized actin cytoskeleton 0.397281539disorganized anterior cerebellum 0.131260176dissociated embryonic cortical neurons 0.193723284dissociated rat cerebellar purkinje neurons 0.247367219dissociated rat cerebellar purkinje neurons 0.247367219dissociated rat cerebellar purkinje neurons 0.247367219dissociated rat hippocampal neurons 0.193089773distal axonal loss 0.150219568distal axons 0.164112709distal purkinje cell dendrites 0.309592383disturbed plasticity 0.148166512dominant cerebellar ataxia 0.221324056dopamine synapses 0.047542422dorsolateral cerebral cortex 0.206095641drug-induced plasticity 0.152037319dynamic recycling 0.247143593dynamin central region 0.223373138dynamin subfamily 0.32867916dynamin subfamily members 0.293381498dynamin superfamily 0.229229169dystrophic axons 0.206164946early contractures 0.056756123early endocytic compartment 0.102581035early endocytic compartments 0.159484393early endocytic events 0.138390389early endocytic pathway 0.140336601early endocytic transport pathway 0.14369396early endocytosis 0.20960571early endosomal canine 0.103081303early endosomal compartment 0.004754781early endosomal compartments 0.054534601early endosomal fusion 0.032839675

early endosomal marker 0.160582847early endosomal markers 0.125491483early endosomal structures 0.06422657early postnatal cerebellar external granular layer 0.220740151early postnatal cerebellum 0.188285588early-onset macrocephaly 0.104242742early-onset neurologic phenotype 0.159168994early-onset severe neurologic phenotype 0.154368424ectopic axons 0.091213059ectopic mossy fiber synapses 0.182780537ectopic mossy fiber synapses 0.182780537ectopic projection 0.03177272ectopic synapses 0.109329666elastic connections 0.160598907electric organ postsynaptic membrane 0.277700703electrical synapses 0.186826047elevated endosomal cholesterol content 0.052832474embryonic cortical neurons 0.190966306embryonic murine cerebellum 0.047982353embryonic neuronal migration 0.268533506embryonic olfactory bulb 0.125759668embryonic rat cortical neurons 0.216508505embryonic rat hippocampal neurons 0.18610359embryonic rat neocortex 0.17286775embryonic xenopus spinal axons 0.017030231endocytic accessory protein 0.128416713endocytic activity 0.196780807endocytic apparatus 0.203680805endocytic carrier vesicles 0.21625804endocytic coated vesicles 0.228208164endocytic codes 0.194741895endocytic compartment 0.121080119endocytic compartments 0.182415451endocytic cycle 0.0324endocytic delivery pathway 0.157234436endocytic dendritic cell compartments 0.20544649endocytic event 0.195593219endocytic lysosomal pathways 0.041290964endocytic machinery 0.196159217endocytic membrane 0.267085279endocytic membrane fusion 0.191093233endocytic membrane retrieval 0.260135615endocytic membrane trafficking 0.230054149endocytic membranes 0.24968456endocytic multivesicular bodies 0.197812882endocytic organelles 0.217809323

endocytic pathway 0.150222199endocytic pathway factors 0.035083919endocytic pathways 0.122150583endocytic process 0.206983119endocytic processes 0.230936652endocytic protein 0.128350022endocytic protein complexes 0.110033072endocytic protein scaffolds 0.13870741endocytic proteins 0.125648082endocytic receptors 0.03144endocytic recycling 0.231647686endocytic recycling 0.231647686endocytic recycling compartment 0.166053902endocytic recycling compartment 0.166053902endocytic recycling machinery 0.223657925endocytic recycling machinery 0.223657925endocytic recycling pathway 0.186043894endocytic recycling pathway 0.186043894endocytic route 0.208731444endocytic system 0.17700508endocytic traffic 0.194789344endocytic trafficking 0.151038558endocytic transport 0.132747171endocytic uptake 0.091498818endocytic vacuoles 0.186001184endocytic vesicles 0.218360322endocytic zone 0.208263264endocytosis inhibition 0.263037745endocytosis modulation 0.233728601endocytosis pathway 0.178103096endogenous ampa glutamate receptors 0.04665121endogenous gabaergic differentiation 0.131125757endogenous nmdar activation 0.002572018endogenous synapses 0.155813287endosomal activation 0.001512251endosomal aggregate formation 0.142528645endosomal biogenesis 0.147477362endosomal cathepsin proteases 0.004754781endosomal chloride concentration 0.106130726endosomal cholesterol transport 0.025339796endosomal clustering 0.117140288endosomal clustering 0.117140288endosomal compartment 0.018347221endosomal compartments 0.071515401endosomal dynamics 0.153654741endosomal expression 0.1559022

endosomal group 0.07839218endosomal lipid transfer proteins 0.044559958endosomal localization 0.156686857endosomal marker protein 0.134934906endosomal markers 0.141986628endosomal membrane 0.21899673endosomal membranes 0.145718944endosomal pathway 0.087123694endosomal protein 0.057926988endosomal protein sorting 0.076302206endosomal recycling 0.13409992endosomal recycling 0.13409992endosomal recycling compartment 0.090936812endosomal recycling compartment 0.090936812endosomal recycling pathway 0.13096486endosomal recycling pathway 0.13096486endosomal recycling system 0.121739665endosomal recycling system 0.121739665endosomal retention 0.03075851endosomal sorting 0.079807927endosomal sorting complex 0.083112049endosomal sorting complex protein 0.0772863endosomal sorting motif 0.086724025endosomal sorting proteins hrs 0.096648627endosomal structures 0.084891197endosomal system 0.049562708endosomal targeting 0.233945372endosomal tlr activation 0.04769593endosomal trafficking 0.077999765endosomal transport 0.083901062endosomal transportation 0.052777702endosomal tubules 0.09640493endosomal vacuoles 0.042609982endosomal vesicle protein 0.104701857endosomal vesicles 0.149493418endothelial cytoskeleton 0.225171833enhanced actin fiber formation 0.409038215enhanced actin filament 0.391029902enhanced ampa receptor-mediated transmission 0.082795701enhanced clustering 0.153615739enhanced synaptic activity 0.193800775enhanced synaptic plasticity 0.179084555enlarged bulb region 0.157397829entorhinal cortex 0.238476505entorhinal cortex 0.238476505entorhinal cortical neurons 0.22224247

entorhinal cortical neurons 0.212924092erythrocyte cytoskeleton 0.407066557erythrocyte membrane cytoskeleton 0.379166952erythroid cytoskeleton 0.153813613evoked postsynaptic potentials 0.132684846evoked transmitter release 0.050422385exceptional familial clustering 0.017063477excessive actin stress fibers 0.410248406excitatory cortical neurons 0.1947213excitatory glutamatergic synapses 0.125487915excitatory postsynaptic current properties 0.129451095excitatory postsynaptic currents 0.110224462excitatory postsynaptic sites 0.104912539excitatory projection neurons 0.154518214excitatory pyramidal cells 0.133831026excitatory synapse development 0.106189536excitatory synapse formation 0.181951314excitatory synapse number 0.150364491excitatory synapses 0.132239124expressed parvalbumin 0.168019668extraordinary plasticity 0.146674156facial motor neurons 0.187268034f-actin cytoskeleton 0.368256797familial clustering 0.0214873fast anterograde axonal transport 0.189632743fast axonal transport 0.178905682fast motor neurons 0.198747725fast retrograde axonal transport 0.198791821fast synaptic inhibition 0.221379975fast synchronous transmitter release 0.1171989fetal cerebellum 0.165221347fetal neocortex 0.107337229fiber synapses 0.245531461filamentous actin 0.380682796filamentous actin cytoskeleton 0.391911828filamentous actin cytoskeleton 0.391911828focal neurologic deficits 0.155303645focal neurologic symptoms 0.125393943forebrain gabaergic interneurons 0.13132262forebrain gabaergic neurons 0.164781745form functional synapses 0.199130152form synapses 0.20396334frontal cortex pyramidal neurons 0.18301174frontal cortex pyramidal neurons 0.18301174frontal cortex samples 0.184342726frontal cortex tissue 0.204295826

frontal neocortex 0.15856375frozen frontal cortex brain tissue 0.197272789functional ampa receptors 0.073894511functional nicotinic synapses 0.140387625functional nmda receptors 0.081877879functional plasticity 0.150940143functional postsynaptic response 0.098596825functional synapses 0.185252482functional synaptic connections 0.184025101functional synaptic connections 0.184025101functionally mature rat hippocampal pyramidal neurons 0.204601994functionally mature rat hippocampal pyramidal neurons 0.204601994functionally normal synapses 0.208794813future cerebral cortex 0.21430968gaba-a receptor-mediated postsynaptic inhibition 0.152880976gabaergic amacrine cells 0.149749186gabaergic blockade 0.156004569gabaergic boutons 0.197884245gabaergic cortical interneurons 0.212716949gabaergic currents 0.134599116gabaergic granule cells 0.188771829gabaergic inhibition 0.229996074gabaergic inhibitory interneurons 0.144673939gabaergic inhibitory neurons 0.171246039gabaergic interneurons 0.142474596gabaergic markers 0.232189127gabaergic neurons 0.171930961gabaergic neurotransmission 0.149947628gabaergic synaptic transmission 0.183478555gabaergic synaptic transmission 0.183478555gabaergic system 0.159708557gabaergic terminals 0.176596765gabaergic transmission 0.15929536gabaergic vesicle distribution 0.22680209general endocytic machinery 0.176701135general synapse organization 0.266917839genome plasticity 0.053629336giant axonal neuropathy 0.132408429glutamate ampa receptor-mediated synaptic transmission 0.080618136glutamate ampa receptors 0.045727227glutamate n-methyl-d-aspartate receptors 0.074623819glutamatergic presynaptic short-term plasticity 0.115226054glutamatergic synapses 0.155838445glycinergic synapses 0.187797334glycinergic synaptic activity 0.190438735granule cell axons 0.268981686

gtpase dynamin 0.296814017guide neuronal cell migration 0.329585514hair bulb 0.095511842hair cell afferent synapse 0.237688711hair cortex 0.195249174hair follicle bulb 0.089631372healthy adult human hippocampus 0.177036179hepatocyte cytoskeleton 0.323496297heterotopic purkinje cells 0.200954335high chromatin plasticity 0.109086599high synaptic activity 0.191335458higher neurologic severity 0.181896019high-frequency signal transmitter 0.063304238highly conserved pdz domain 0.112835744highly specialized cytoskeleton 0.394296679hippocampal ampa receptors 0.031755613hippocampal mossy fiber axons 0.218452227hippocampal mossy fiber axons 0.218452227hippocampal mossy fiber long-term potentiation 0.16692603hippocampal mossy fibers 0.253683329hippocampal nmda receptors 0.042956959hippocampal plasticity 0.142350851hippocampal pyramidal cell layer 0.262568805hippocampal pyramidal cell layers 0.249793921hippocampal pyramidal cells 0.171236849hippocampal pyramidal neurons 0.17006254hippocampal synapses 0.167061198hippocampal synaptic plasticity 0.170115331hippocampus blocks long-term potentiation induction 0.138601003hippocampus cdna library 0.08186273hippocampus expression library 0.210313636hippocampus fire 0.177139399hippocampus formation 0.220030975hippocampus library 0.178887299hippocampus mrna 0.149584372homogeneous severe neurologic phenotype 0.214237558homologous integral membrane 0.177146838host cell actin reorganization 0.399690791human adrenal cortex library 0.243468201human adult hippocampus cdna library 0.136461905human brain cortex microglia 0.238059479human cerebellar 0.287582171human cerebellar cortex 0.297898976human cerebellar cortex 0.297898976human cerebellar granule cell lineage 0.2478135human cerebellar purkinje cells 0.256061305

human cerebellar purkinje cells 0.256061305human cerebellum 0.286106897human cerebellum cdna library 0.123940915human cerebellum library 0.275389667human cerebral cortex 0.258229762human cerebral cortex library 0.256011143human cerebral cortex mrna 0.19980266human cortex 0.265373632human cortical dysplasias 0.323685017human cortical networks 0.330475183human cortical neurons 0.239421651human developing cortical neurons 0.228251911human fetal adrenal cortex 0.211376139human forebrain cortex library 0.231804679human frontal cortex gray matter 0.187855993human frontal cortex library 0.218438092human hippocampus 0.235845701human hippocampus cdna 0.123259261human hippocampus cdna libraries 0.123378392human hippocampus cdna library 0.116236095human hippocampus library 0.230237407human kidney cortex 0.23157356human kidney cortex microsomes 0.315687492human neurologic disorders 0.234629232human neuronal cell death 0.27243271human neuronal cell lines 0.295473938human neuronal ceroid lipofuscinosis 0.269055268human neuronal diseases 0.255961489human neuronal migration defects 0.300271716human neurons 0.200195584human paraneoplastic neurologic disorders 0.229100963human parietal neocortex 0.21660129human temporal cortex 0.238162961human tensin 0.302899187hypoplastic cerebellum 0.241199284immature immunologic synapse 0.164468975immature synapses 0.156734805immune clustering 0.017063477immune synapse 0.048689522immune synapse formation 0.09505328immune synapses 0.089213823immunoglobulin-like integral membrane glycoproteins 0.222651375immunologic synapse 0.251037392immunologic synapse formation 0.266518571immunologic synapses 0.198387736immunological synapse 0.24194668

impaired axonal transport 0.146746636impaired clustering 0.06791266impaired endocytic activity 0.151328996impaired endocytosis 0.175099195impaired gaba synaptic inhibition 0.200813285impaired hippocampal plasticity 0.130540366impaired neuronal migration 0.282273221impaired pdz 0.162930736impaired plasticity 0.111666808impaired protein endocytosis 0.139389307impaired recycling 0.121451043impaired sg assembly 0.119174843impaired synaptic plasticity 0.166187283inappropriate synaptic connections 0.178746625increased ampa currents 0.109397022increased cerebral cortical surface area 0.28898919increased gabaergic activity 0.192342923increased local actin polymerization 0.385941421increased presynaptic transmitter release 0.119755297increased synapses 0.208023191increased synaptic norepinephrine release 0.189622667independent neurologic deficits 0.206318953individual hippocampal pyramidal cells 0.171293756individual neuronal migration 0.299505782individual synapses 0.18315734infantile neurologic onset 0.188182971infantile neuronal ceroid lipofuscinosis 0.255069421inferior cerebellar hypoplasia 0.211203534inhibitory gabaergic differentiation 0.140729725inhibitory gabaergic interneurons 0.144673939inhibitory gabaergic nerve terminals 0.174273897inhibitory gabaergic neurotransmission 0.145249898inhibitory gabaergic system 0.150263359inhibitory postsynaptic current frequency 0.096743214inhibitory postsynaptic membrane formation 0.264740277inhibitory synapse 0.175981422inhibitory synapse development 0.130037278inhibitory synapses 0.172985162insoluble cytoskeleton 0.37184195integral balance 0.052956979integral branch 0.145177354integral cell membrane 0.270295216integral cell membrane glycoprotein 0.2212403integral component 0.183361097integral components 0.08734528integral erythrocyte membrane protein 0.209831022

integral golgi membrane protein 0.17972124integral growth component 0.007694002integral intermediate filament component 0.189370259integral member 0.145654193integral membrane 0.250573847integral membrane domain 0.189273634integral membrane glycoprotein 0.177566526integral membrane glycoproteins 0.249766754integral membrane microsomal enzyme 0.322924693integral membrane part 0.251105232integral membrane protein 0.195735822integral membrane protein ii 0.143374538integral membrane proteins 0.194980701integral membrane proteoglycans 0.24905803integral membrane receptors 0.004693671integral membrane transporter protein 0.077913859integral mitochondrial membrane protein 0.171996532integral number 0.190256653integral part 0.180669913integral parts 0.172764532integral peroxisomal membrane protein 0.174535565integral protein 0.060281359integral proteins 0.065488115integral proteolytic fragment 0.00654026integral regulatory component 0.042595024integral role 0.118022821integral roles 0.095424234integral structural component 0.162175993integral subunit 0.004693671integral transmembrane protein 0.078076733integral type ii membrane glycoproteins 0.098596565integrin endocytosis 0.02657575intermediate filament cytoskeleton 0.273460785intermediate recycling pathway 0.159253014internal cytoskeleton 0.383136856interphase cytoskeleton 0.31144983intracellular actin 0.397290847intracellular actin microfilaments 0.397918533intracellular recycling 0.165916196intrinsic glutamatergic connections 0.13924021ion channel clustering 0.005770448ipsilateral projection 0.089212812iron recycling 0.01369191irregular connections 0.236518431isolated actin 0.381032289isolated axons 0.184134208

isolated cerebellar 0.249202484joint contractures 0.090593729juvenile-onset cortical cataract mapping 0.258954523kainate receptor-mediated excitatory postsynaptic currents 0.084836288keratin cytoskeleton 0.124575756kidney cortex 0.190763229laminar projection 0.271565062lamprey central synapse 0.230874958late endocytic membranes 0.232727553late endocytic organelles 0.199346055late endocytic pathway 0.144373767late endocytic structures 0.191042893late endocytic traffic 0.176476633late endocytic trafficking 0.142630778late endocytic trafficking defects 0.121240492late endocytic transport 0.12857886late endocytic vesicles 0.206563732late endosomal 0.043171855late endosomal compartment 0.011228752late endosomal membranes 0.133306671late endosomal protein 0.051834367late endosomal structures 0.072111956late neurologic presentation 0.196758259late-onset cerebellar 0.231602619late-onset cerebellar ataxia 0.21388339late-onset neurologic syndrome 0.151279089late-onset pure cerebellar ataxia 0.213887218lateral cerebellar 0.229050035lateral motor column neurons 0.184452041lateral septal cortex 0.184342726lens cortical fiber cell membranes 0.277038577ligand endocytosis 0.039358041lineage plasticity 0.05588728local cortical blood flow 0.258614566localized actin assembly 0.381072421localized neuronal migration disorder 0.281310234long actin fibers 0.420288445long actin stress fibers 0.413399464long axons 0.188040418long-lasting inhibitory postsynaptic potentials 0.133633382long-term plasticity 0.136482882long-term plasticity independent 0.128753871long-term synaptic plasticity 0.168978201low-density lipoprotein endocytosis 0.028652058lower cortical layers 0.281019336lower motor neurons 0.190641016

lumbar lateral motor neurons 0.183767826lysosomal integral membrane glycoprotein 0.138537734macromolecular pdz protein 0.15850452macrophage recycling 0.005372161main olfactory bulb 0.194897401major axonal tracts 0.152683122major conserved integral membrane proteins 0.166317709major cortical axonal tracts 0.251167558major projection areas 0.173277932major synaptic vesicle membrane 0.239548959mammalian cerebral cortex 0.19351644mammalian neocortex 0.155368712mating projection defect 0.032477727mature cerebellar purkinje cells 0.238211321mature cerebellar purkinje cells 0.238211321mature cerebellar purkinje neurons 0.227148687mature cerebellar purkinje neurons 0.227148687mature cerebellar purkinje neurons 0.227148687mature cerebellum 0.272198769mature cortex 0.235971184mature cortical networks 0.293798147mature immunologic synapse forms 0.242445011mature motor neurons 0.195556127mature neuronal marker 0.284754906mature olfactory neuronal phenotype 0.264693618mature purkinje cells 0.200815743mature synapses 0.202073864medium spiny projection neurons 0.161776072melanoma clustering 0.24318955membrane recycling 0.268962071membrane-associated spectrin cytoskeleton 0.285038483membrane-bound vacuole 0.093599721microtubule cytoskeleton 0.201960388mild axonal distal neuropathy 0.126672722mild cerebellar 0.262966111mild cerebellar ataxia 0.235316581mild cerebellar atrophy 0.220559772mild cerebellar findings 0.260719735mild neurologic abnormalities 0.207661305mild neurologic involvement 0.228327026mild permanent cerebellar 0.245415965mild pyramidal signs 0.222496452miniature excitatory postsynaptic current frequency 0.085509161miniature excitatory postsynaptic currents 0.114638103mitochondrial axonal transport 0.15468674mitochondrial clustering 0.098848862

moderate cerebellar atrophy 0.21486549modified cytoskeleton 0.390910582modulating synaptic plasticity 0.18098639monomeric actin 0.382176745monosynaptic connections 0.131267663mossy cells 0.180221306mossy fiber axons 0.245898908mossy fiber axons 0.245898908mossy fiber long-term potentiation 0.180221306mossy fiber projection 0.274768567mossy fiber projection 0.274768567mossy fiber stimulation 0.182251032mossy fiber synapses 0.244278552mossy fiber synapses 0.244278552mossy fiber synaptic strength 0.215002628mossy fiber synaptic strength 0.215002628mossy fiber synaptic transmission 0.199869723mossy fiber synaptic transmission 0.199869723mossy fiber terminal complexes 0.214434587mossy fiber terminals 0.251220888mossy fibers 0.326475919motor axons 0.228029683motor cortex 0.269397958motor neuron axonal degeneration 0.146211828motor neuron axons 0.212516258motor neurons 0.188354697motor synaptic terminals 0.20859464mouse ampa glutamate receptors 0.031584545mouse axonal fiber tracts 0.143837933mouse cerebellum 0.1155389mouse cerebellum extracts 0.102356774mouse hippocampus 0.113770952mouse lateral olfactory tract axons 0.132988876mouse motor axons 0.152597931mouse neocortex 0.155368712mouse neurologic mutation 0.105159343mouse neuronal synapses 0.205525977mouse olfactory bulb expression library 0.147658756mouse primary cortical neurons 0.193747941mouse purkinje cells 0.160595605mouse sensory axons 0.113536352multiple axons 0.18644488multiple congenital contractures 0.182306626multiple contractures 0.138358348multiple contractures mapping 0.146309164multiple dilated cortical tubules 0.322523796

multiple joint contractures 0.101111108multiple neuronal cell types 0.283223415multiple neuronal markers 0.268086081multiple pdz domains 0.178823141multiple synapses 0.190824861multiple synaptic forms 0.183610563multiple synaptic vesicles 0.200708287murine cerebellum 0.04667727muscle actin 0.381744363muscle contractures 0.224369377mutant actin isoforms 0.391128474mutant cerebellum 0.09714789mutant purkinje cells 0.159212749mutant synapses 0.107645478mutational clustering 0.063404437myelinated axonal segments 0.131308989myelinated axons 0.169503038nasal axons 0.166180353native ampa receptors 0.080071431native synaptic vesicles 0.196761003neocortex impaired neuronal positioning 0.24882271neonatal rat cortical neurons 0.21144096nephrogenic cortex 0.211454047nervous system plasticity 0.107250754neural connections 0.05354191neural membrane recycling 0.242373734neural plasticity 0.100285397neural transmitter release 0.074896394neurologic abnormalities 0.201269449neurologic actions 0.180144586neurologic complications 0.19669029neurologic conditions 0.228157727neurologic death 0.159701503neurologic decline 0.209700052neurologic defect 0.170769411neurologic defects 0.173808601neurologic deficit 0.210143172neurologic deficits 0.215909443neurologic development 0.154693459neurologic diseases 0.22133722neurologic disorder 0.137848963neurologic disorders 0.190350183neurologic disorders mapping 0.202636407neurologic distress 0.204094591neurologic disturbances 0.215929459neurologic dysfunction 0.189261456

neurologic evaluation 0.238239756neurologic examination 0.224601207neurologic illness 0.20451674neurologic impairment 0.220589958neurologic improvement 0.218643202neurologic involvement 0.217629399neurologic manifestations 0.218725515neurologic motor impairment 0.26430372neurologic mutation 0.105980571neurologic outcome 0.190837774neurologic phenotype 0.233581422neurologic phenotypes 0.170563558neurologic point 0.221218955neurologic problems 0.221762648neurologic recovery 0.215830601neurologic signs 0.226113832neurologic status 0.183970699neurologic symptoms 0.173595695neurologic syndrome 0.155387142neurologic tissues 0.243535043neurologic variant 0.231958737neuromuscular synapse 0.275299918neuromuscular synapses 0.222076572neuronal adhesion 0.26736847neuronal adhesion molecule 0.275090485neuronal branching 0.244575144neuronal cell adhesion 0.287666486neuronal cell adhesion molecule 0.290172996neuronal cell adhesion molecules 0.288460224neuronal cell bodies 0.283267086neuronal cell body 0.279036073neuronal cell contact 0.299072998neuronal cell culture 0.284060993neuronal cell cultures 0.292271672neuronal cell death 0.258505798neuronal cell development 0.255463349neuronal cell extracts 0.274816184neuronal cell guidance 0.291636157neuronal cell injury 0.268084449neuronal cell layers 0.295760215neuronal cell lineages 0.27853939neuronal cell lines 0.29102248neuronal cell loss 0.277253011neuronal cell migration 0.321718186neuronal cell polarity 0.274990385neuronal cell populations 0.28510871

neuronal cell proliferation 0.266753685neuronal cell subtype 0.259211688neuronal cell surface 0.30173476neuronal cell survival 0.295229538neuronal cell types 0.287219774neuronal connections 0.230550181neuronal cytoskeleton 0.365209694neuronal cytoskeleton 0.365209694neuronal enzyme 0.30511453neuronal expression 0.261081103neuronal fibers 0.29892556neuronal fibers characteristic 0.297733297neuronal inhibition 0.254653124neuronal intermediate filaments 0.269967254neuronal isoform 0.265714096neuronal layer 0.254819507neuronal localization 0.26897334neuronal markers 0.26798688neuronal membrane protein 0.278628335neuronal membranes 0.261323909neuronal migration 0.300696815neuronal migration abnormalities 0.293033647neuronal migration defect 0.285441022neuronal migration defects 0.28151902neuronal migration disorder 0.267978176neuronal migration errors 0.294224489neuronal molecules 0.271748398neuronal plasma membrane marker 0.294315816neuronal plasticity 0.232947604neuronal postsynaptic densities 0.229621602neuronal primary cultures 0.261939879neuronal surface 0.274439587neuronal surface membranes 0.299154647neuronal survival 0.270701812neuronal survival machinery 0.275356757neuronal survival signal 0.259035359neuronal synapse 0.260958282neuronal synapse 0.260958282neuronal synapses 0.249198997neuronal synaptic activity 0.239766838neuronal synaptic plasticity 0.223572529neuronal synaptic plasticity 0.223572529neuronal synaptic transmission 0.221181666neuronal targeting 0.305073496neuronal wiskott-aldrich syndrome protein 0.270539682nicotinic fast excitatory postsynaptic currents 0.140626644

nk cell-activating immunologic synapse 0.097659094nmda agonist 0.039433723nmda channels 0.027359534nmda currents 0.043482141nmda glutamate receptors 0.054113736nmda receptor-mediated excitatory postsynaptic potentials 0.069744966nmda receptor-mediated excitatory postsynaptic potentials 0.069744966nmda receptor-mediated field responses 0.007466745nmda receptor-mediated glutamate toxicity 0.021341451nmda receptor-mediated neurotransmission 0.029458585nmda receptor-mediated synaptic currents 0.098412713nmda receptors 0.07677242nmda responses 0.0176918nmda synaptic receptors 0.002380303nmda toxicity 0.04991284nmdar activation 0.006925786nmdar activity 0.094089258nmdar channel 0.012712314nmdar channel activity 0.031244425nmdar complex 0.076905526nmdar excitatory currents 0.060341617nmdar hypofunction 0.062619612nmdar maximum open probability 0.067026848nmdar responses 0.002472727nmdar stimulation 0.055704642nmdar subunit 0.015649119nmdar tyrosine phosphorylation 0.002343727n-methyl-d-aspartate receptors 0.056276676n-methyl-d-aspartate stimulation 0.056276676n-methyl-d-aspartate subtype 0.000654246normal actin organization 0.38873884normal adrenal cortex 0.214571614normal basal synaptic transmission 0.195241254normal cerebellar 0.221996158normal cerebellar tissue 0.229657832normal cerebellum 0.217477622normal cerebral cortex 0.211901296normal clustering 0.136661712normal fetal adrenal cortex 0.183818324normal human cerebral cortex 0.241674979normal human neurologic development 0.188466836normal human ovarian cortex 0.206498781normal human temporal cortex 0.216271427normal mature cortical neurons 0.214201118normal motor neurons 0.192105417normal neonatal neurologic examination 0.149388198

normal neurologic 0.198450274normal neurologic development 0.147818399normal olfactory bulb architecture 0.190127193normal projection 0.133918511normal short-term synaptic plasticity 0.174288296normal synapse 0.220332285normal synapse formation 0.233175638normal synapses 0.204295254normal synaptic properties 0.197870238n-terminal pdz domain 0.08815706nuclear clustering 0.004437874observed decreased tubular endocytosis 0.265791165observed neurologic benefit 0.222474658ocular dominance plasticity 0.144708219oculomotor axons 0.177162643olfactory axons 0.210579576olfactory bulb 0.19625425olfactory bulb defects 0.136235121olfactory bulb interneuron diversity 0.156632388olfactory bulb interneurons 0.157459771olfactory bulb mitral cells 0.152030463olfactory bulb neurogenesis 0.090785565olfactory bulb neurons 0.177403847olfactory cortex 0.240580121olfactory nerve projection 0.18188071onion bulb formation 0.200532855organized actin filaments 0.381791771osteoclast cytoskeleton 0.151818708outer cortex 0.241224907parallel actin bundles 0.39085878paraneoplastic neurologic diseases 0.217393754paraneoplastic neurologic disorder 0.135222974paraneoplastic neurologic disorders 0.186873833paraneoplastic neurologic symptoms 0.170626523partial gabaergic blockade 0.149583747parvalbumin characteristic 0.17286775patient cerebellar tissue 0.282525202patient frontal cortex 0.19870909pdz adaptors 0.185912491pdz domain 0.133576575pdz domain binding region 0.029039186pdz domain interaction site 0.100102562pdz domain-containing golgi protein 0.136502977pdz domain-containing phosphoprotein 0.211044085pdz domain-containing protein 0.140529842pdz domain-containing proteins 0.137576626

pdz domains 0.181699258pdz interactions 0.152712546pdz ligand 0.030632408pdz motif 0.197118827pdz motifs 0.204557054pdz proteins 0.148377058pdz region 0.195207034pdz sequences 0.119323739pdz type ii ligand motif 0.012972628pediatric neurologic service 0.201538775perinatal human cortex 0.252078369peripheral axonal motor 0.18043879peripheral axons 0.136736083peripheral cell cortex 0.223587214peroxisomal integral membrane protein 0.174535565phagocytic synapse 0.352734242phagocytic vacuole 0.195670099photoreceptor cell axons 0.113859764photoreceptor cortical actin cytoskeleton 0.332297056photoreceptor cortical actin cytoskeleton 0.332297056photoreceptor synapse 0.027036467photoreceptor synapses 0.04942179physiologic synaptic activity 0.191536023pigment cell-specific integral membrane glycoprotein 0.188286778piriform cortex 0.211535472plasma membrane recycling 0.185365248plasticity mechanisms 0.153817336plasticity process 0.163903497platelet integral membrane glycoprotein 0.082523052podocyte cytoskeleton 0.346925175polarized actin bundles 0.395682794posterior cerebellum 0.119945722postmortem cortex samples 0.234651124postmortem cortical tissue 0.278499367postmortem hippocampus 0.185838003postnatal cortex 0.196630653postnatal glutamatergic synapse development 0.159638798postnatal motor neurons 0.186096567postnatal mouse cerebellar granule cell neurons 0.208943406postnatal mouse cerebellar granule cell neurons 0.208943406postsynaptic action 0.141679295postsynaptic activity 0.169753811postsynaptic adhesion 0.236290163postsynaptic ampa receptors 0.025974822postsynaptic ampa receptors 0.022227272postsynaptic apparatus 0.158031071

postsynaptic atp-sensitive potassium channels 0.023337608postsynaptic calcium 0.187393441postsynaptic calcium channels 0.080585465postsynaptic calcium ion sensor 0.160989009postsynaptic calcium transients 0.205818699postsynaptic cells 0.165524992postsynaptic central nervous system neurons 0.157939895postsynaptic clathrin-coated vesicles 0.20185994postsynaptic compartment 0.132156968postsynaptic components 0.150727994postsynaptic congenital myasthenic syndrome 0.183258277postsynaptic congenital myasthenic syndromes 0.211200354postsynaptic current 0.14423682postsynaptic currents 0.127310419postsynaptic cytoskeleton 0.311504103postsynaptic cytoskeleton 0.311504103postsynaptic decrease 0.164463132postsynaptic densities 0.149161819postsynaptic density 0.159265091postsynaptic density complex 0.165925588postsynaptic density fraction 0.166603435postsynaptic density fractions 0.168352965postsynaptic density preparations 0.163458823postsynaptic density proteins 0.155479136postsynaptic density scaffold 0.165443464postsynaptic differentiation 0.14790977postsynaptic domain 0.132861341postsynaptic dopaminergic neurons 0.132646484postsynaptic elements 0.099838853postsynaptic endocytic events 0.1968123postsynaptic endocytic events 0.1968123postsynaptic enzymes 0.208104498postsynaptic events 0.141025486postsynaptic expression 0.213953236postsynaptic glycine 0.141190652postsynaptic inhibition 0.198650889postsynaptic junctions 0.152106644postsynaptic layer 0.20104773postsynaptic lipid raft fractions 0.134804348postsynaptic localization 0.208820212postsynaptic markers 0.205098971postsynaptic maturation 0.138731834postsynaptic membrane 0.269545823postsynaptic membrane trafficking 0.246221539postsynaptic membranes 0.197083185postsynaptic molecules 0.218992814

postsynaptic neuromuscular junction 0.176911445postsynaptic neuron 0.172013009postsynaptic neurons 0.177157914postsynaptic nmda receptors 0.037196003postsynaptic nmda receptors 0.02117744postsynaptic plasma membranes 0.159441756postsynaptic plasticity 0.153003711postsynaptic plasticity 0.153003711postsynaptic pool 0.154143235postsynaptic protein sorting 0.158478682postsynaptic proteins 0.145982224postsynaptic reception apparatus 0.154982199postsynaptic region 0.15341515postsynaptic regions 0.123949721postsynaptic responses 0.084618493postsynaptic role 0.159983474postsynaptic scaffolding 0.181192921postsynaptic side 0.159814087postsynaptic signaling 0.049662375postsynaptic signaling mechanisms 0.055712031postsynaptic site 0.143134895postsynaptic sites 0.127857728postsynaptic specialization 0.151516786postsynaptic spectrin cytoskeletons 0.15348763postsynaptic spikes 0.148851526postsynaptic spiral ganglion neurons 0.176585787postsynaptic structures 0.162023885postsynaptic surface expression 0.245687744postsynaptic target 0.218060231postsynaptic terminal 0.159726398postsynaptic terminals 0.155342039postsynaptic trafficking 0.157939443potassium recycling 0.019280188potential endocytosis motif 0.249031352potential endocytosis signal 0.197010153predominant cerebellar ataxia 0.230125859premature axonal differentiation 0.136276293presynaptic excitatory synapses 0.126929947presynaptic plasticity 0.126000259presynaptic short-term plasticity 0.109086599presynaptic short-term synaptic plasticity 0.153099025presynaptic synapses 0.152802006presynaptic transmitter release 0.095588832primary axons 0.238737958primary cortical areas 0.3276174primary cortical cell cultures 0.310267912

primary cortical cultures 0.329592037primary cortical neurons 0.223909189primary cultured motor neurons 0.215485663primary cultured neurons 0.191076473primary cultured rat hippocampal neurons 0.205270249primary enteric rat neurons 0.19180192primary frontal cortex cell cultures 0.265559915primary human neurons 0.218321189primary mouse cortical neurons 0.193747941primary neuronal cell cultures 0.302628176primary neuronal cultures 0.261939879primary rat cerebellar granule cells 0.239905629primary rat cortical neurons 0.23976673primary rat hippocampal neurons 0.203021804primary rat neurons 0.203762334primary rat sympathetic neurons 0.20005599primary somatosensory cortex 0.271982947primary synaptic clefts 0.200798052primary visual cortex 0.244514344priming synaptic vesicles 0.187928308principal actin isoform 0.403241667principal excitatory synapses 0.138198837progressive joint contractures 0.05114242progressive macrocephaly 0.111291356progressive neurologic condition 0.199842976progressive neurologic developmental disorder 0.105159996progressive neurologic disorder 0.124170903progressive neurologic dysfunction 0.167059939progressive neurologic impairment 0.184892216progressive neurologic phenotype 0.213669488progressive neurologic phenotypes 0.151566392progressive neurologic symptoms 0.152436251progressive severe contractures 0.062223011project axons 0.183033832projection fiber bundles 0.334469476projection mouse neurons 0.144802936projection neuron dendrites 0.21884397projection neurons 0.164315795proper clustering 0.184353641proper neuronal migration 0.299534107proper synaptic connections 0.183189682proper synaptic connections 0.183189682proprioceptive afferent axons 0.170640382protein integral 0.060281359proximal axonal enlargement 0.177973252proximal axonal segments 0.145647534

proximal axons 0.182932665proximal motor axons 0.228616422proximal tubular endocytosis 0.217428541punctate endocytic structures 0.216448381pure cerebellar 0.249822009pure cerebellar ataxia 0.225330059pure cerebellar signs 0.250584489purkinje cell 0.297821582purkinje cell antigens 0.200779936purkinje cell axon terminals 0.317155156purkinje cell axonal swellings 0.301288729purkinje cell axonal swellings 0.301288729purkinje cell bodies 0.294855173purkinje cell cytoplasm 0.296091859purkinje cell death 0.264463226purkinje cell degeneration 0.217545892purkinje cell degeneration phenotype 0.234364757purkinje cell dendrites 0.320533465purkinje cell dendritic trees 0.261320615purkinje cell development 0.252864478purkinje cell layer 0.312838353purkinje cell layers 0.303304429purkinje cell lineage 0.238334696purkinje cell loss 0.286324117purkinje cell maturation 0.265348627purkinje cell nuclei 0.282782309purkinje cell pathology 0.274395999purkinje cell precursor number 0.279940767purkinje cell spiking 0.298759309purkinje cells 0.201216274purkinje cell-specific deletion 0.171289357purkinje cell-specific promoter 0.195158082purkinje fibers 0.333721554purkinje layer 0.285072275purkinje neuron axonal swellings 0.243335186purkinje neuron axonal swellings 0.243335186purkinje neurons 0.206623865purkinje neurons 0.206623865pyramidal cell dendrites 0.266615157pyramidal cell layer 0.264524281pyramidal cell layers 0.243306329pyramidal cells 0.127498931pyramidal cortical layer 0.293142682pyramidal cortical layer 0.293142682pyramidal damage 0.075767375pyramidal distal dendrites 0.223030385

pyramidal dysfunction 0.120286761pyramidal layer 0.274416024pyramidal layers 0.251564147pyramidal neuron dendritic spines 0.207206195pyramidal neurons 0.170665536pyramidal shape 0.277070674pyramidal signs 0.220352293pyramidal symptoms 0.0918871pyramidal tract involvement 0.149674309pyramidal tract signs 0.18421886pyramidal tracts 0.189529657rabbit olfactory bulb 0.127336124radial neuronal migration 0.298851816rapid actin polymerization 0.384359273rapid cytoskeleton binding 0.133946299rapid endosomal tubular movement 0.142216894rapid neurologic regression 0.165520269rapid retrograde synaptic modification 0.184545075rat brain cerebellum microsomal membranes 0.262289661rat brain cortical neurons 0.221928017rat brain cytoskeleton 0.271501906rat brain synapses 0.213591253rat brain synaptic soluble fractions 0.224881637rat brain synaptic vesicles 0.238469894rat brainstem neurons 0.185572155rat cerebellar cortex 0.248791884rat cerebellar cortex 0.248791884rat cerebellar granular neurons 0.222856608rat cerebellar granular neurons 0.222856608rat cerebellar granule neurons 0.226068649rat cerebellar granule neurons 0.226068649rat cerebellar sections 0.223531419rat cerebellar tissues 0.222857342rat cerebellum 0.207404418rat cerebellum cdna expression library 0.157180492rat cerebellum cdna library 0.133904014rat cerebral cortex 0.197710526rat cerebral cortex membrane fractions 0.274727099rat cerebral cortex synaptosomes 0.209435856rat cortex 0.194269239rat cortical neurons 0.227260339rat embryonic cortical neurons 0.216508505rat embryonic hippocampal neuronal cell bodies 0.261756339rat glutamatergic neurons 0.19192235rat hippocampal neurons 0.190979621rat hippocampal neurons multiple sumoylation targets 0.205522983

rat hippocampal pyramidal neurons 0.196642133rat hippocampal pyramidal neurons 0.196642133rat hippocampal synaptic vesicles 0.222806775rat hippocampus 0.175451498rat hippocampus cdna library 0.12382857rat hippocampus library 0.17425286rat integral membrane glycoprotein 0.213838165rat kidney cortex 0.182650825rat neocortex 0.178814708rat neuronal cell cultures 0.284599464rat neuronal cell lines 0.285787738rat neurons 0.188844544rat olfactory bulb 0.190958296rat olfactory bulb output neurons 0.202840312rat olfactory bulb output neurons 0.202840312rat primary cortical neurons 0.23976673rat primary hippocampal neurons 0.203021804rat pyramidal neurons 0.198203747rat pyramidal neurons 0.198203747rat superior cervical ganglion neurons 0.192482792rat sympathetic neurons 0.18589519receptor-mediated endocytic functions 0.098399844receptor-mediated endocytic pathway 0.117676416receptor-mediated endocytosis 0.152743614reciprocal inhibitory synapses 0.170125997recycling endocytic pathway 0.186043894recycling endocytic pathway 0.186043894recycling endosome 0.204335431recycling endosome compartment 0.117139922recycling endosome marker 0.292231003recycling endosomes 0.170355536recycling endosomes supply ampa receptors 0.090936812recycling endosomes supply ampa receptors 0.045742091recycling pathways 0.091819774reduced cerebellar mass 0.257031306reduced endocytosis 0.240889328reduced neuronal cell volume 0.289461014reeler neuronal migration defects 0.299465504regulated endocytosis 0.259850214regulatory synapse 0.09079237relative macrocephaly 0.230083065repetitive synaptic activity 0.183630405residual cortex 0.223631782retinal axons 0.072328791retinal ganglion cell axonal projections 0.144492315retinal ganglion cell axons 0.137361914

retinal projection 0.033240261retrograde axonal transport 0.179577045retrograde synaptic 0.190814093ribosome recycling 0.132161064rodent cerebral cortex 0.196920737rodent cortex 0.200157717rodent hippocampus 0.159195772rodent neuronal cell cultures 0.284177868rodent neuronal precursor cell lines 0.273027368screening adult human occipital cortex 0.244387783secondary axonal loss 0.151664586selective neuronal cell loss 0.259134556sensorimotor axonal polyneuropathy 0.164722541sensorimotor connections 0.171596265sensory afferent axons 0.149871435sensory ataxic neuropathy 0.098758274sensory axons 0.154225141sensory cerebellar cortices 0.224791602sensory cortex 0.202088234severe ataxic phenotype 0.176035194severe autosomal recessive neurologic disorder 0.135857024severe cerebellar ataxia 0.216490857severe cerebellar granule cell loss 0.266757219severe cobblestone cortex 0.220609933severe contractures 0.10834695severe infantile neurologic form 0.258816412severe neurologic 0.213030978severe neurologic defects 0.16556648severe neurologic deficits 0.20270992severe neurologic disorder 0.13459457severe neurologic dysfunction 0.181876754severe neurologic impairment 0.20610963severe neurologic phenotype 0.217697225severe neurologic symptoms 0.166279965severe neurologic syndrome 0.148150096severe neuronal cell death 0.258547046severe paraneoplastic cerebellar ataxia 0.21287032sg assembly 0.209871952sg formation 0.193672035sg mutation 0.1148632sg phenotype 0.159481661short actin clusters 0.392311958short actin fibers 0.421394837short actin filaments 0.383561216short-term plasticity 0.10942456short-term plasticity characteristics 0.116717201

short-term plasticity properties 0.138646784short-term synaptic plasticity 0.163013773signaling connections 0.004602208single motor neurons 0.184404246single pdz domain 0.121595804site-directed actin assembly 0.380857486skeletal motor neurons 0.213812654small cerebellum 0.351996189small cerebral cortex 0.262569975small clear synaptic vesicles 0.220390861small cortex 0.297276351small integral membrane 0.285341263small integral membrane glycoproteins 0.281195383small integral membrane subunit 0.152126753small ipsilateral projection 0.262025107small stable actin patches 0.394555003small synaptic vesicles 0.21933167smooth muscle actin 0.394376246soluble integral component 0.096652976somatic synapses 0.165781867somatosensory cortex 0.219782459sort integral membrane 0.250893168specialized postsynaptic endocytic mechanism 0.221083485specialized postsynaptic endocytic mechanism 0.221083485specific axons 0.178123441specific excitatory synapses 0.133218712specific neurologic syndromes 0.240033283specific neuronal membrane proteins 0.269930471specific phosphoinositides direct actin polymerization 0.382339765spinal motor axons 0.165588481spinal respiratory plasticity 0.098471475spinal sensory axons 0.108269384spine plasticity 0.20381026spine structural plasticity 0.219989723spine synapses 0.22145727spontaneous gabaergic inhibitory postsynaptic currents 0.130744806spontaneous gabaergic inhibitory postsynaptic currents 0.130744806spontaneous inhibitory postsynaptic responses 0.059311737spontaneous miniature excitatory postsynaptic current frequency 0.058724864stable immunologic synapse formation 0.26475024structural clustering 0.172287137structural plasticity 0.167754948subcortical actin cytoskeleton 0.401420882subcortical actin cytoskeleton 0.401420882subcortical axonal projections 0.15764208

superficial cortex 0.240905684superficial cortical layers 0.286652269surface clustering 0.216255716survival motor neurons protein complex 0.218187127sustained structural plasticity 0.160658607sustained synaptic activity 0.186621941sympathetic axonal projections 0.178341125synapse activation 0.037249609synapse assembly 0.28179592synapse development 0.134924369synapse expansion 0.18607296synapse formation 0.26368181synapse loss 0.19842883synapse modification 0.162897755synapse number 0.266483857synapse specificity 0.203489309synapse stability 0.228510076synapse ultrastructure 0.261491509synapses increases phosphorylation 0.105044424synaptic active zone 0.185041005synaptic activity 0.189342391synaptic activity inhibitors 0.185653712synaptic adhesion 0.257028967synaptic adhesions 0.185961594synaptic alignment 0.184286107synaptic ampa receptors 0.01593663synaptic basal lamina 0.205872316synaptic basement membranes 0.227962711synaptic calcium 0.197641268synaptic cell adhesion 0.281599351synaptic cell adhesion molecules 0.284329682synaptic clustering 0.181259433synaptic clusters 0.183022566synaptic complex critical 0.182774805synaptic complex formation 0.198917377synaptic connections 0.180656271synaptic contacts 0.191140067synaptic cytosol 0.193200366synaptic dysfunction phenotype 0.200951974synaptic expression 0.227163359synaptic fractions 0.181422222synaptic inhibition 0.212099461synaptic junctions 0.18962895synaptic layer 0.205393264synaptic level 0.185253736synaptic localization 0.212428277

synaptic markers 0.213541379synaptic membrane 0.250596197synaptic membrane fractions 0.253261004synaptic membrane fusion 0.214663688synaptic membrane proteins 0.236442661synaptic membranes 0.200417366synaptic motor neuron boutons 0.215103273synaptic nmda receptors 0.002380303synaptic nmdar localization 0.185451706synaptic nmdar localization 0.185451706synaptic n-methyl-d-aspartate receptors 0.007642349synaptic organization 0.187507846synaptic pdz proteins 0.190835941synaptic pdz proteins 0.190835941synaptic plasma membrane fraction 0.224962838synaptic plasma membrane vesicles 0.228031497synaptic plasma membranes 0.185142092synaptic plasticity 0.173021478synaptic plasticity processes 0.181855022synaptic regulation 0.193940118synaptic scaffolding proteins 0.191105208synaptic soluble fraction 0.182827197synaptic spines 0.193204201synaptic stress 0.184200466synaptic structures 0.183067535synaptic targeting 0.231658924synaptic vesicle assembly 0.194922809synaptic vesicle cluster 0.19348511synaptic vesicle clustering 0.18599845synaptic vesicle clustering 0.18599845synaptic vesicle clusters 0.187851174synaptic vesicle endocytosis 0.212878114synaptic vesicle endocytosis 0.212878114synaptic vesicle fraction 0.185822278synaptic vesicle fractions 0.187385227synaptic vesicle invagination 0.187169817synaptic vesicle marker 0.217694424synaptic vesicle marker recycling 0.231782065synaptic vesicle marker recycling 0.231782065synaptic vesicle membrane 0.241315731synaptic vesicle precursors 0.186534173synaptic vesicle recycling 0.198434553synaptic vesicle recycling 0.198434553synaptic vesicle surface 0.210870708synaptic vesicles 0.197652323synaptic vesicular membrane 0.261545332

tagged synapses 0.184230257target neurons 0.200660789temporal cortex 0.186276635temporal retinal axons 0.068676896tensin phosphorylation 0.2104142tensin polypeptide 0.212643176terminal actin filaments 0.381913048terminal axons 0.188332099terminally differentiated cerebellar 0.251106284thalamic area 0.108942635thalamic gaba-a receptors 0.108942635thalamic infusion 0.03736675thalamic interneurons 0.098651678thalamic neurons 0.157514573thalamic nuclei 0.043344421thalamic nucleus 0.028759722thalamic reticular nucleus 0.018902456thin axonal neurites 0.201444423thin axonal processes 0.231985797thin cortical plates 0.322066263thin long actin filaments 0.38864486thoracic level motor neurons 0.185286471tight clustering 0.257890756tonic neuronal inhibition 0.266237628total anomalous pulmonary venous connections 0.08631988transferrin endocytosis 0.060671221transferrin recycling 0.02343572transient macrocephaly 0.138073transmitter glutamate 0.095588832transmitter release 0.074215851tubular endocytosis 0.23766778tubular recycling endosomes 0.184624095type ii integral membrane 0.083074281type ii integral membrane protein 0.084112873type ii integral transmembrane protein 0.013265995type ii pdz domains 0.021179691type ii pdz ligand 0.021100797typical pyramidal shapes 0.23549249undifferentiated outer cortex 0.264299095unilateral cerebellar atrophy 0.222709624unilateral cerebellar hypoplasia 0.215565031unique neurologic disorder 0.142662407unmyelinated axons 0.184039586unmyelinated axons 0.184039586unmyelinated fibers 0.316716463unmyelinated nerve fibers 0.247506757

unmyelinated parallel fibers 0.314503476unmyelinated sensory axons 0.17272737unmyelinated sensory axons 0.155229096unrelated neurologic disorders 0.189226937unwanted synapses 0.184178437upper cortex region 0.223983728upper cortical layer neurons 0.221298544upper layer neurons 0.183758504upper motor neurons 0.190227107vacuole characteristics 0.0560081vacuole development 0.0512442vacuole formation 0.133522788vacuole membranes 0.154073335vacuole sorting protein 0.07409813variable neurologic 0.210906756variant late infantile neuronal ceroid lipofuscinosis 0.266219859vascular actin 0.399235391vascular endocytosis 0.262220094ventral root axons 0.089130509ventral root motor axons 0.153895398ventral tegmental area gabaergic neurons 0.137871614ventricular purkinje fibers 0.288458575vertebral visual projection 0.073110153vertebrate synapses 0.16584192vesicle endocytosis 0.212232595vesicle recycling 0.178292629visual cortex 0.198312575visual cortical plasticity 0.255380235visual plasticity 0.137489526visual system plasticity 0.131544029vocal plasticity 0.154096155xenopus axons 0.0142427yeast endosomal 0.055482204

Table S20. Textrous!-based extracted noun-phrases from differential cortical T1R3KO versus WT control protein expression data. For each noun-phrases extracted using Textrous! the associated Cosine Similarity Score is given.

Noun-Phrase Cosine Similarity Score myosin storage myopathy 0.215058717aberrant cellular trafficking dynamics 0.098309978aberrant cofactor exchange mechanism 0.01559597aberrant collagen vi microfilament formation 0.01559597aberrant cytoskeletal organization 0.145688685abnormal contraction 0.007761689abnormal cytoskeletal actin organization 0.232166175abnormal exchange 0.040796114abnormal f-actin organization 0.208136623abnormal filopodia 0.124108352abnormal motility 0.111787255acetylated actin 0.251856759acid exchange 0.094471632actin anchorage sites 0.238522531actin component 0.239460345actin core 0.239638557actin crosslinking activity 0.252401021actin crosslinking protein 0.246291231actin cytoskeletal abnormalities 0.233097997actin cytoskeletal assembly 0.243214763actin cytoskeletal assembly 0.243214763actin cytoskeletal network 0.248461563actin cytoskeletal network 0.248461563actin cytoskeletal organization 0.248867461actin cytoskeletal organization 0.248867461actin cytoskeletal regulation 0.229819001actin cytoskeletal reorganization 0.253492724actin cytoskeletal reorganization 0.253492724actin cytoskeletal reorganization 0.253492724actin cytoskeleton 0.261755901actin cytoskeleton 0.261755901actin cytoskeleton organization 0.260144455actin cytoskeleton organization 0.260144455actin cytoskeleton rearrangement 0.246346241actin cytoskeleton rearrangement 0.246346241actin dynamics 0.245004586actin dynamics 0.245004586actin fiber disassembly 0.239265451actin fibers 0.261172828actin filament 0.239334535

actin filament bundles 0.239752197actin filament bundles 0.239752197actin filament dynamics 0.241477032actin filament dynamics 0.241477032actin filament length 0.239199696actin filament network 0.236458725actin filament organization 0.237799963actin filament structures 0.240492799actin filament subunits 0.373722589actin filaments 0.373722589actin filaments anchor 0.240933059actin fold 0.245610296actin interface 0.252729031actin isoforms 0.28440551actin kinetics 0.24082693actin microfilaments 0.240477624actin microspikes 0.244144854actin modeling 0.242524314actin module results 0.243562849actin molecule 0.262631241actin monomer 0.240035359actin motion 0.244083665actin myopathy 0.243019324actin network 0.240695594actin network disassembly 0.235976474actin networks 0.24151364actin organization 0.241338915actin polymerization 0.238618919actin polymerization 0.238618919actin polymerization studies 0.233536283actin polymers 0.240714987actin release 0.27277853actin reorganization 0.247152187actin reorganization 0.247152187actin sequence 0.243297408actin sequestration 0.236343729actin staining 0.267119101actin stress fibers 0.25395052actin structures 0.24446424actin subunits 0.393023983actin tails 0.244711036actin thin filaments 0.238463916actin thin filaments 0.238463916actin-binding ability 0.236883581actin-binding activity 0.227241088actin-binding domain 0.208232843

actin-binding domains 0.2657443actin-binding interfaces 0.251921712actin-binding molecules 0.182384161actin-binding motif 0.214675276actin-binding motifs 0.221180585actin-binding n-terminal domain 0.149271226actin-binding protein 0.182718031actin-binding proteins 0.148218592actin-binding proteins ezrin 0.257765595actin-binding region 0.205789786actin-binding repeat region 0.257765595actin-binding sequences 0.113905203actin-binding sites 0.167761443activated gtpase 0.187189381activation contraction 0.067962357activation dynamics 0.070442431active guanine nucleotide exchange factor 0.257765595active guanine nucleotide exchange factor 0.079649828actomyosin cytoskeleton 0.277777332actomyosin cytoskeleton 0.277777332actomyosin network 0.139167404actomyosin ring 0.128658554alpha-actin genotype 0.044166716alpha-actin synthesis 0.187242915alpha-synuclein polymerization 0.040488529altered channel dynamics 0.051945816altered ciliary motility 0.132023187altered contractility 0.091338642alternative nonmuscle exon 0.109555097amino acid exchange 0.088615938ancient outer transmembrane gtpase 0.143109837anion exchange 0.057803157anion exchange activity 0.002702916anion exchange inhibitor 0.017016605anterior maxillary protrusion 0.3452775antiviral guanine analogs 0.028581156aorta contractile responses 0.090574619aortic contraction 0.033967382apical bundles 0.001828914aplysia ras-related homologs 0.011597458atypical guanine nucleotide exchange factor 0.095665534autocrine motility factor 0.070854531autonomous contraction 0.104522794axon bundles 0.254987125axonal bundles 0.128192275axonal cytoskeleton 0.251333372

axonal microtubule bundles 0.028787713basal epithelial cell protrusions 0.038148649basal gtpase activity 0.188508851basal myocardial contractility 0.095555277base exchange 0.026632355beta cardiac myosin heavy chain genes 0.099000093beta human cardiac myosin heavy chain genes 0.093942697beta myosin heavy chain 0.193678171biliary tract motility 0.128315546bind actin 0.252121327bind f-actin 0.300170846bind nonmuscle actin filaments 0.249452404bind nonmuscle actin filaments 0.249452404bind phosphoinositides 0.171143642binding monomeric gtpases 0.124626154binding phosphoinositides 0.257567568bovine n-wasp cdna fragments 0.115550322brain gtpase-activating protein 0.191105417brush border cytoskeleton 0.268165183calcium dynamics 0.255645474canalicular cytoskeletal proteins 0.18376463cancer cell cytoskeleton 0.078409108cardiac alpha-actin 0.004478969cardiac contractile apparatus 0.008492615cardiac contractile performance 0.000848468cardiac contractile proteins 0.013352821cardiac contractile responses 0.001071327cardiac contractility 0.008088089cardiac contraction 0.008911679cardiac cytoskeleton 0.31122172cardiac muscle alpha-actin 0.060033266cardiac muscle contraction 0.050759369cardiac muscle myosin 0.156311722cardiac myofilament 0.31122172cardiac myofilaments 0.31122172cardiac myosin 0.147473068cardiac myosin heavy chain beta 0.132436242cardiac myosin heavy chain genes 0.11355501cardiac myosin heavy chains 0.152372153cardiac myosin heavy-chain dimer 0.153643003cation exchange chromatography 0.045757383caveolae dynamics 0.009740616cell contraction 0.013105441cell dynamics 0.014131891cell filopodia 0.051470189cell junction dynamics 0.06074936

cell membrane protrusions 0.066129958cell motility 0.048861531cell protrusion 0.006227017cellular bundles 0.095829012cellular contraction 0.11797879cellular exchange 0.070677952cellular filopodia 0.20832405cellular motility 0.169808462cellular myosin heavy chain 0.231870448cellular protrusions 0.116641877central gtpase domain 0.196407491central guanine nucleotide exchange factor domain 0.030250402central guanine nucleotide exchange factor domain 0.030250402central myosin tail homology region 0.252420592c-fos reorganization 0.00152928chain polymerization 0.142619803characteristic myosin homology region 0.241622239characteristic n-terminal myosin head 0.237701983chicken smooth muscle tropomyosin 0.168065694cholesteryl ester exchange 0.041280006chondroitin polymerization 0.205035458chromatin dynamics 0.065284283chromatin reorganization 0.215191974chromosomal dynamics 0.019709701chromosomal exchange 0.02719092chromosome binding dynamics 0.008587358cilia motility 0.171206149ciliary motility 0.160813977cilium motility 0.199950196class iii myosins 0.053090875class ix myosins 0.215191974clinically apparent motility disorders 0.097747681cochlear hair bundles 0.046388338cofactor exchange 0.096543363collagen bundles 0.013204619complete guanine nucleotide exchange factor catalytic cycle 0.072371679complete guanine nucleotide exchange factor catalytic cycle 0.072371679complex cytoskeletal networks 0.204224567conformational dynamics 0.216779815consensus gtpase motifs 0.159330577conserved gtpase domain 0.140782267conserved gtp-binding domain 0.181212221conserved gtp-binding domains 0.196023127conserved gtp-binding elongation factor signature 0.007274805conserved gtp-binding site 0.173859302conserved muscle contractile protein myosin light chain kinase 0.175513636

conserved muscle contractile protein myosin light chain kinase 0.175513636conserved n-terminal globular gtp-binding domain 0.160504212conserved n-terminal gtpase domain 0.116624945continuous actin-binding domain 0.205418176contractile activity 0.163898477contractile apparatus 0.097218617contractile bands 0.124132514contractile cycle 0.028672469contractile cytoskeleton 0.281033313contractile cytoskeleton 0.281033313contractile deficiency 0.032296509contractile dysfunction 0.028282243contractile element 0.027097036contractile elements 0.031214707contractile force 0.14651089contractile impulse 0.075731488contractile marker 0.083981464contractile myoepithelial cells 0.045327905contractile performance 0.132698938contractile phenotype 0.215191974contractile properties 0.170918957contractile protein 0.144416867contractile proteins 0.106625396contractile reserve 0.135024735contractile response 0.040941754contractile responses 0.066779892contractile ring 0.117543901contractile ring assembly 0.114726531contractile ring constriction 0.132470562contractile stimulus 0.192840948contractile strength 0.197230905contractile structures 0.139016267contractile structures results 0.124723627contractile system 0.089752768contractile tissue 0.131133385contractile work 0.117835493contraction frequencies 0.056843044core gtpase domain 0.175750169cortical actin 0.262999608cortical actin assembly 0.254799366cortical actin cytoskeleton 0.279453412cortical actin cytoskeleton 0.279453412cortical actin cytoskeletons 0.262968866cortical actin filaments 0.253719027cortical actin formation 0.255621727cortical actin skeleton 0.260378924

cortical actin structures 0.263769675cortical actomyosin contraction 0.188607086cortical actomyosin contraction 0.188607086cortical cytoskeleton 0.280902637cortical f-actin 0.278170128critical wiskott-aldrich syndrome region 0.056288219c-terminal actin-binding domain 0.199206209c-terminal golgi dynamics domain 0.160526605c-terminal gtpase 0.205232863c-terminal gtpase domain 0.193468741c-terminal gtp-binding domain 0.225572593c-terminal myosin tail 0.271533438c-terminal myosin tail motif 0.272837788cytoplasmic actin gels 0.237973717cytoplasmic cytoskeletal filaments 0.170342929cytoskeletal abnormalities 0.146675167cytoskeletal actin 0.250293891cytoskeletal actin 0.250293891cytoskeletal actin bundle 0.247670414cytoskeletal actin bundle 0.247670414cytoskeletal actin organization 0.248867461cytoskeletal actin organization 0.248867461cytoskeletal activity 0.25061595cytoskeletal adaptor 0.160488983cytoskeletal adaptor ezrin 0.239209863cytoskeletal alpha-actinin 0.316593772cytoskeletal architecture 0.233642897cytoskeletal assembly 0.208833775cytoskeletal attachment 0.242468802cytoskeletal attachments 0.235644854cytoskeletal behaviors 0.232236583cytoskeletal calyx 0.250381885cytoskeletal complex 0.211911585cytoskeletal components 0.201402939cytoskeletal core proteins 0.156030995cytoskeletal crosslinking protein 0.191819123cytoskeletal disruption 0.204086437cytoskeletal dynamics 0.242402176cytoskeletal dynamics 0.242402176cytoskeletal effector 0.127956634cytoskeletal elements 0.174974323cytoskeletal extracts 0.221425372cytoskeletal filament networks 0.175416083cytoskeletal filaments 0.187759735cytoskeletal fraction 0.254504911cytoskeletal framework 0.243865838

cytoskeletal interactions 0.226744142cytoskeletal intermediary 0.233299587cytoskeletal intermediate filaments 0.172622282cytoskeletal isoform 0.260069112cytoskeletal linker 0.206241405cytoskeletal localization 0.207130775cytoskeletal matrix 0.044827903cytoskeletal modeling 0.238390335cytoskeletal network 0.226363984cytoskeletal organization 0.230556233cytoskeletal pathways 0.151666469cytoskeletal polarity 0.122168325cytoskeletal polarization 0.243661717cytoskeletal protein 0.210275446cytoskeletal protein actin monomeric 0.25387402cytoskeletal protein actin monomeric 0.25387402cytoskeletal protein ankyrin 0.240490012cytoskeletal protein spectrin 0.208995822cytoskeletal protein talin 0.205229256cytoskeletal proteins 0.174374608cytoskeletal proteins actin 0.243676298cytoskeletal proteins actin 0.243676298cytoskeletal rearrangement 0.139737241cytoskeletal rearrangements 0.178192327cytoskeletal receptors 0.185457246cytoskeletal regulation 0.125773058cytoskeletal regulators 0.173282005cytoskeletal regulatory protein 0.124038439cytoskeletal regulatory proteins 0.101578063cytoskeletal remodeling 0.17626025cytoskeletal remodeling mechanisms 0.170863756cytoskeletal reorganization 0.252726089cytoskeletal reorganization 0.252726089cytoskeletal restructuring 0.241133387cytoskeletal scaffolds 0.255409365cytoskeletal stability 0.200228791cytoskeletal staining 0.265896145cytoskeletal structures 0.23699875cytoskeletal vimentin 0.19506205cytoskeleton architecture 0.295096592cytoskeleton arrangement 0.305080983cytoskeleton linker protein 0.231125388cytoskeleton organization 0.288782808cytoskeleton proteins 0.210893339cytoskeleton regulation 0.181539457cytosolic actin-binding proteins 0.100992469

dc motility 0.018285669de novo guanine nucleotide biosynthesis 0.001516531decreased contractility 0.141839029decreased gtpase activity 0.215191974decreased lamellipodia protrusion 0.207004614decreased lamellipodia protrusion 0.207004614decreased left ventricular contractility 0.006810736decreased wound contractile forces 0.078366735defective actin polymerization 0.215599606defective polymerization 0.005672405dendritic cell motility 0.075672958dendritic cellular protrusions 0.119833494dendritic protrusions 0.111948216dense bundles 0.1655463depressed contractility 0.144959333descending thin limbs 0.07512707detrusor muscle contraction 0.135438322dictyostelium myosin heavy chain kinase 0.16237774dimerization dynamics 0.1863891direct exchange 0.00548944disabled motility 0.204914298disorganized actin cytoskeleton 0.25939875disorganized actin cytoskeleton 0.25939875dna polymerization 0.215191974dna strand exchange activity 0.079343393domain exchange experiments 0.131883451dynamic cytoskeletal localization 0.21462575dynamic exchange 0.118366244dynamic f-actin structures 0.297764041dynein motility 0.146306414edge dynamics 0.23511707embryonic myosin 0.196683617embryonic skeletal myosin heavy chain 0.175633241emerging protrusion 0.124404724endogenous myosin heavy chain 0.209456959endogenous myosin va 0.217700332endosomal dynamics 0.09625166endosome motility 0.195802846endothelial cell filopodia 0.013543768endothelial cell motility 0.014584487endothelial cytoskeleton 0.057933829enhanced actin fiber formation 0.248586209enhanced actin filament 0.251694551enhanced basal contractility 0.118600268enhanced cardiac contractile performance 0.013540968enhanced contraction 0.16330288

enzyme dynamics 0.142702841epigenetic dynamics 0.029904443epithelial cell motility 0.012636025epithelial cell-specific gtpase 0.006704092epithelial protrusions 0.208422404erythrocyte cytoskeleton 0.310018775erythrocyte membrane cytoskeleton 0.230913848erythroid cytoskeleton 0.071076447erythroid-specific cytoskeletal protein 0.208755175essential cytoskeletal structures 0.153312936eukaryotic cytoskeletal machinery 0.212287933excess endothelial filopodia 0.017995504excessive actin stress fibers 0.254684877exchange activity 0.129332787exchange event 0.041628227exchange events 0.022902617exchange kinetics 0.057656683exchange l-serine 0.067040211exchange mechanism 0.067993037exchange points 0.083666541exchange proteins 0.07415911exchange rate 0.062431997exchange rates 0.059365231exchange reaction 0.101948066exchange substrates 0.039287069exchange transfusion 0.084069089f-actin abnormalities 0.205248755f-actin assembly 0.257998167f-actin binding 0.171462614f-actin binding domain 0.175796019f-actin bundles 0.29618114f-actin bundles 0.29618114f-actin conformational transition 0.280837493f-actin content 0.301506913f-actin content change 0.310173494f-actin core 0.237658791f-actin crosslinking 0.251634575f-actin cytoskeleton 0.311558627f-actin cytoskeleton 0.311558627f-actin dynamics 0.293551226f-actin dynamics 0.293551226f-actin filaments 0.231048959f-actin level 0.285807935f-actin organization 0.287173594f-actin recruitment 0.182889409f-actin staining 0.32482992

f-actin stress fibers 0.237123836f-actin structures 0.293250392fast skeletal muscle contractile protein genes 0.089197519fast voltage-dependent motility 0.241965853fetomaternal exchange 0.067293308fiber bundles 0.1139654fibroblast filopodia 0.209908939fibrous protein tropomyosin 0.152114023filament bundles 0.120217775filamentous actin 0.239603004filamentous actin crosslinking protein 0.242949596filamentous actin cytoskeleton 0.258507884filamentous actin cytoskeleton 0.258507884filamentous cytoskeletal network 0.208422404filamentous f-actin 0.27489941filopodia formation 0.204296327filopodia number 0.273388002flanking bundles 0.068922947frap analysis 0.208422404frap technique 0.18148842frontal thin bands 0.073662882functional gtpase 0.140840795fundamental f-actin cytoskeletal defects 0.190590328fundamental f-actin cytoskeletal defects 0.190590328gallbladder contraction 0.071921967gastric motility 0.041078998gastrointestinal motility 0.171376876gastrointestinal smooth muscle contractility 0.162703657gdp-gtp exchange 0.065311056gdp-gtp exchange activity 0.129944729gdp-gtp exchange reaction 0.10202073golgi dynamics domain 0.15143939golgi membrane dynamics 0.130270888greater cell motility 0.050539516growth cone motility 0.007412834gtp exchange 0.058415688gtp exchange protein 0.105513469gtpase accelerating protein 0.188320266gtpase activating protein 0.165945254gtpase activating protein activity 0.176492258gtpase activators 0.144699203gtpase activities 0.193387266gtpase activity 0.209908939gtpase activity assay 0.183666695gtpase binding 0.126742192gtpase binding site 0.124787736

gtpase controls nucleocytoplasmic transport 0.209908939gtpase coordination 0.185357959gtpase domain 0.194216455gtpase dynamin 0.30230145gtpase effector domain 0.146569456gtpase exchange 0.164246579gtpase exchange 0.164246579gtpase impairment 0.163781351gtpase motifs 0.18426297gtpase partner 0.188549969gtpase regulator 0.128599796gtpase regulators 0.131084664gtpase signaling cascades 0.003166799gtpase superfamily 0.150391614gtpase targets 0.164278007gtpase-activating domain 0.189397665gtpase-activating domain domain 0.168828527gtpase-activating protein 0.167913466gtpase-activating protein activity 0.176387616gtpase-activating protein domain 0.173530552gtpase-activating proteins 0.123190963gtpase-activating site 0.219892295gtp-binding activity 0.234794067gtp-binding assay 0.206382897gtp-binding cytoskeletal proteins 0.218435368gtp-binding cytoskeletal proteins 0.218435368gtp-binding domain 0.22487882gtp-binding domains 0.247220284gtp-binding elongation factor signature 0.007274805gtp-binding motif 0.214025739gtp-binding motifs 0.213715008gtp-binding protein 0.220048716gtp-binding protein domain 0.217255611gtp-binding proteins 0.186418316gtp-binding site 0.198323794gtp-dependent allosteric activators 0.198323794guanine deaminase 0.084883064guanine diphosphate 0.073253646guanine exchange factor 0.198323794guanine nucleotide 0.041134guanine nucleotide binding 0.045912358guanine nucleotide dissociation stimulator 0.066192716guanine nucleotide dissociation stimulators 0.066670965guanine nucleotide exchange 0.050729952guanine nucleotide exchange 0.050729952guanine nucleotide exchange activities 0.057929324

guanine nucleotide exchange activities 0.057929324guanine nucleotide exchange activity 0.093231383guanine nucleotide exchange activity 0.093231383guanine nucleotide exchange factor 0.093078669guanine nucleotide exchange factor activity 0.054905233guanine nucleotide exchange factor activity 0.054905233guanine nucleotide exchange factors 0.036681756guanine nucleotide exchange factors 0.036681756guanine nucleotide exchange protein 0.089629332guanine nucleotide exchange protein 0.089629332guanine nucleotide pathway 0.009760516guanine nucleotide regulatory protein 0.047549696guanine nucleotide release 0.140699269guanine nucleotide-binding protein 0.098516305guanine nucleotide-binding proteins 0.061531268guanine nucleotides 0.0818399guanine positions 0.084814281guanine residues 0.108218746guanine ring 0.080504964guanine-nucleotide exchange factor domain 0.053699373guanosine triphosphate exchange 0.125115387gut contractile activity 0.138782354gut motility 0.171411814hair bundles 0.019757095heart contractility 0.026534227heart muscle contractility 0.08917645helical bundles 0.054521178hepatic vascular dynamics 0.036735193hepatocyte cytoskeleton 0.24299936heterotrimeric gtp-binding protein 0.205596747heterotrimeric gtp-binding proteins 0.175203116heterotrimeric guanine nucleotide-binding protein 0.09347877heterotrimeric guanine nucleotide-binding proteins 0.060834748higher gtpase activities 0.181284141higher gtpase activity 0.198323794highly conserved gtp-binding domain 0.175188599highly conserved guanine 0.000320831highly conserved n-terminal gtpase domain 0.112762101highly related gtpases 0.176205573highly specialized cytoskeleton 0.286715252histone exchange 0.024385481histone methylation dynamics 0.198323794homologous class iii myosins 0.040952879host cell actin reorganization 0.186314154human cardiac actins 0.198323794human cytoskeletal isoform 0.196539814

human guanine nucleotide exchange factor 0.088766154human myosin 0.229551094human myosin heavy chain 0.213447178human myosin light chain kinase 0.153238592human myosin protein 0.241778725human myosin vi 0.209481691human nonmuscle myosin heavy chain 0.217495699human nonmuscle myosin heavy chain 0.217495699human radixin cdna 0.061600469human syncytiotrophoblast microvillus cell surface membranes 0.014430781human wasp sequence 0.192540554hydrogen exchange 0.123363883hydrogen exchange studies 0.073175808hydrolyzing phosphoinositides 0.181432223immune cell motility 0.004630201immunosuppressive drug rapamycin 0.098525517impaired beta-adrenergic contractile responses 0.054548527impaired cardiac contractility 0.014355317impaired cell motility 0.035644665impaired contractility 0.051581194impaired motility 0.140290533increased cell motility 0.072050673increased contractility 0.130347428increased filopodia density 0.221902734increased local actin polymerization 0.251506427increased local actin polymerization 0.251506427increased motility 0.201977052increased muscle contraction 0.154271726increased myosin specificity 0.244947229inhibited contraction 0.178078468inhibitory gtp-binding regulator 0.248891788inhibitory gtp-binding regulators 0.250064203insoluble cytoskeletal preparations 0.199075506insoluble cytoskeleton 0.265576197intergenic sequence exchange 0.040947491intermediate filament cytoskeletal network 0.156946762intermediate filament cytoskeleton 0.190720376intermediate sister chromatid exchange levels 0.049420777internal cytoskeleton 0.305775966internal exchange substrate 0.066137476interphase cytoskeleton 0.224452074intracellular actin 0.252915143intracellular actin microfilaments 0.250861906intracellular actin-binding proteins 0.143580953intracellular bundles 0.1014079intracellular cytoskeletal proteins 0.169058894

intracellular gtp-binding proteins 0.186416269intracellular motility 0.166385831intracellular retrograde motility 0.197643493intrinsic gtpase activity 0.207360222intrinsic gtpase defect 0.096702182irreversible exchange 0.098573569isolated actin 0.242806964isometric contractile strength 0.188697488isometric contractile strength 0.188697488isometric force 0.187052676isometric force generation 0.135685892isometric force production 0.107928572isotope exchange kinetics 0.05777jejunal contraction 0.070795299keratin cytoskeleton 0.065614876kinetochore microtubule dynamics 0.010114385labeled hair bundles 0.001846905lamellipodia formation 0.107928572lamellipodia protrusion 0.261444946lamellipodia protrusion 0.261444946lamellipodia tips 0.273236536latent gtpase activity 0.164905148left ventricular contractility 0.032919408leukocyte cytoskeletal organization 0.149835041listeria motility 0.133243252localized phosphorylation dynamics 0.132864318long actin fibers 0.256528559long actin stress fibers 0.249339063long cellular filopodia 0.179028782long filopodia 0.237577965low basal gtpase activity 0.177855498low contractile performance 0.109776193low gtpase activity 0.196601814low intrinsic gtpase activity 0.194946953lower intrinsic gtpase activity 0.205646373major contractile protein 0.129773999major cytoskeletal filament systems 0.16623052major cytoskeletal keratin 0.053512006major nonmuscle product 0.188785381mammalian cytoplasmic actins 0.032248711mammalian cytoskeletal proteins 0.137134364mammalian gtpase-activating protein 0.107928572mammalian myosin iiia 0.23538675mammalian sperm motility 0.091345302matrix reorganization 0.014103327mature actin protein 0.242961657

mature hair bundles 0.035425894maximal contractile response 0.049100237maximum isometric force 0.181845948melanin subunit polymerization 0.400045979membrane contractility 0.141840588membrane dynamics 0.139251746membrane gtpase activity 0.19128642membrane phosphoinositides 0.124961462membrane protrusion 0.121258826membrane protrusions 0.135658044membrane-associated spectrin cytoskeleton 0.317043668membrane-bound phosphoinositides 0.13398748microfilament assembly 0.107928572microfilament bundles 0.227613666microfilament bundles 0.227613666microfilament plasma membrane attachment sites 0.079611441microfilament system 0.059648706microtubule bundles 0.031635441microtubule cytoskeletal elements 0.227613666microtubule cytoskeleton 0.081886023microtubule dynamics 0.016852163microtubule polymerization 0.011500972microtubule-associated cytoskeletal proteins 0.074006153microvillus extracts 0.028642555microvillus inclusion disease 0.227613666microvillus inclusions 0.049515981missense exchange 0.053562894mitochondrial dynamics 0.038541757mitochondrial gtpase 0.016343227mitotic contraction 0.227613666mitotic sister chromatid exchange 0.070599296modified cytoskeleton 0.300881575monomeric actin 0.23833441monomeric gtpases 0.198915165monomeric gtp-binding proteins 0.179793153monomeric guanine nucleotide-binding protein 0.093804944monomeric guanine nucleotide-binding proteins 0.059629098motility apparatus 0.188627586motility defect 0.070926738motility defects 0.034487496motility restriction 0.156160921motility suppressor 0.025829965mouse myosin genes 0.227613666mouse myosin phosphatase 0.126905132mouse skeletal alpha-actin cdna 0.031270585mouse skeletal muscle alpha-actin promoter 0.188627586

mouse wasp locus 0.188627586muscle actin 0.241967606muscle actins 0.147847655muscle contraction 0.145596861muscle contraction results 0.147672824muscle f-actin 0.217773729muscle fiber contraction 0.148293596muscle myosin 0.236018434muscle myosins 0.168446367muscle thin filament 0.160832932mutant thin filaments 0.026013013mutant vestibular hair bundles 0.241967606mutated guanine 0.085000866mutational dynamics 0.001541865myocardial contractility 0.096244817myocardial contractility defects 0.018156067myocardial contraction 0.097196041myocyte contractile performance 0.132741777myocyte contractility 0.128206015myofibril necrosis 0.241967606myosin alkali light chain 0.22930925myosin alkali light chains 0.236603647myosin binding 0.241642153myosin filaments 0.240731919myosin genes 0.182030893myosin head 0.244475908myosin head domain 0.261755265myosin head region 0.242340968myosin heavy chain 0.226254421myosin heavy chain cdna clones 0.196506695myosin heavy chain genes 0.172020922myosin heavy chain sequences 0.2154835myosin heavy chains 0.232926649myosin ic 0.249082219myosin ii 0.248601776myosin ii activity 0.269790601myosin ii filaments 0.245982278myosin ii organization 0.248628676myosin iia 0.251064891myosin iib 0.247447337myosin iiia 0.244254896myosin light chain 0.227861966myosin light chain genes 0.168460587myosin light chain kinase 0.158103475myosin light chains 0.235101641myosin locus 0.209309262

myosin molecule 0.269124416myosin motor 0.266635408myosin motor domains 0.285526572myosin motor proteins 0.263970945myosin phosphatase 0.197004978myosin phosphatase activity 0.209446368myosin regulatory light chain 0.206790501myosin regulatory light chains 0.213360406myosin rod 0.226730707myosin rod domain 0.246903054myosin storage myopathy 0.242335897myosin substrate 0.246442404myosin substrates 0.238949836myosin superfamily 0.24111788myosin tail 0.250005624myosin tail domain 0.265643978myosin va 0.234749266myosin va genes 0.180362008myosin vi 0.222423066myosin viia 0.23795123myosin viia functions 0.232337685natural phosphoinositides 0.076246436nerve bundles 0.228960826network reorganization 0.180871452neural wasp 0.179767598neural wiskott-aldrich syndrome protein functions 0.066639512neuronal cytoskeleton 0.390817906neuronal growth cone filopodia 0.190916408neuronal wiskott-aldrich syndrome protein 0.205910283neuron-specific guanine nucleotide exchange factor 0.07997975neuron-specific tropomyosin isoform 0.213342591nonmuscle actins 0.232812295nonmuscle actins 0.232812295nonmuscle cell 0.039064077nonmuscle cell types 0.039461692nonmuscle cells 0.248418736nonmuscle cultured cells 0.006152265nonmuscle eukaryotic cells 0.024079946nonmuscle form 0.238511725nonmuscle isoform 0.223463774nonmuscle isoforms 0.227838117non-muscle isoforms 0.209117142nonmuscle myosin 0.245004337nonmuscle myosin 0.245004337nonmuscle myosin ii 0.250171754nonmuscle myosin ii 0.250171754

nonmuscle myosin ii heavy chain 0.232986916nonmuscle myosin ii heavy chain 0.232986916nonmuscle myosin iia 0.251657896nonmuscle myosin iia 0.251657896nonmuscle myosin iib 0.247914287nonmuscle myosin iib 0.247914287nonmuscle myosins 0.227100321nonmuscle myosins 0.227100321nonmuscle tissues 0.111643754non-muscle tissues 0.248418736normal contractility 0.024786normal cytoskeletal organization 0.11319419normal microtubule dynamics 0.05048171normal motility 0.05890564normal myocardial contractility 0.036608812normal myofibril organization 0.019066684n-terminal actin-binding domain 0.149271226n-terminal actin-binding domains 0.169903203n-terminal actin-binding region 0.115846901n-terminal filamentous actin-binding domain 0.150821543n-terminal gtpase activating protein domain homologous 0.133496107n-terminal gtpase domain 0.153442424n-terminal gtpase-activating domain 0.13527335n-terminal gtpase-activating protein domain 0.137900375n-terminal gtp-binding domain 0.192400418n-terminal gtp-binding site 0.190561062n-terminal myosin domain 0.251615844nuclear envelope dynamics 0.030931249nuclear gtpase 0.093396805nucleotide exchange 0.031584174nucleotide exchange activity 0.085677929nucleotide exchange cycle 0.035280098nucleotide exchange factor activity 0.070195316nucleotide exchange factors 0.06831388nucleotide exchange proteins 0.05750443nucleotide exchange reaction 0.04885208n-wasp activation 0.248418736n-wasp homolog 0.197519043n-wasp molecules 0.216931087n-wasp mrna 0.1308624n-wasp protein 0.214122488osteoclast actin cytoskeletal reorganization 0.205247169osteoclast actin cytoskeletal reorganization 0.205247169osteoclast cytoskeleton 0.099531564outer hair cell bundles 0.026412831oxygen exchange 0.092973548

palmitoylation dynamics 0.360279994parallel actin bundles 0.248418736parallel actin bundles 0.248418736peripheral actin microspikes 0.237604269photoreceptor cortical actin cytoskeleton 0.201780122physiologic contractile phenotype 0.073418522placenta microvillus extracts 0.066917902plasma membrane dynamics 0.084738856plasma membrane ras-related gtpase 0.121131096plasma membrane ras-related gtpase 0.121131096platelet contractility 0.033858348podocyte cytoskeleton 0.266675303podocyte intermediate cytoskeletal protein vimentin 0.183851119podocyte motility 0.19438799polarized actin bundles 0.241122187polarized actin bundles 0.241122187polarized f-actin 0.285656355polymerization reaction 0.177752217poor motility 0.172846665poorly contractile hearts 0.030281633porcine myosin 0.247392035porcine myosin vi 0.226337115positive motility factor 0.248418736postprandial gastrointestinal motility 0.147237412postsynaptic cytoskeleton 0.764891117potential actomyosin regulatory protein 0.248418736potential cytoskeletal proteins 0.164677286predominant myosin 0.245152712presynaptic cytoskeletal matrix 0.211911286primary antigen cardiac myosin 0.144281124primary cytoskeletal protein defects 0.13227992principal actin isoform 0.278124564process dynamics 0.115738927progressive motility 0.137227098projection fiber bundles 0.177284404protein n-wasp 0.214122488protein reorganization 0.143436367protrusion formation 0.085326685pulmonary gas exchange 0.13174581punctate f-actin staining 0.321270131purified actin filaments 0.244910862purified platelet f-actin 0.115738927purified recombinant gtpase-activating protein domain 0.174143854rabbit skeletal muscle alpha-actin 0.124862469rabbit skeletal muscle f-actin 0.162965595radixin associates 0.237405204

radixin deficiency 0.115738927rag gtpases 0.110370928ral guanine nucleotide dissociation stimulator 0.087385949random motility 0.198337179rapamycin activation 0.086833179rapamycin administration 0.15584979rapamycin analog 0.131878396rapamycin derivative 0.121092543rapamycin functions 0.108545138rapamycin inhibitor 0.121699341rapamycin signaling 0.121699341rapamycin treatment 0.126208609rapid actin polymerization 0.2406944rapid actin polymerization 0.2406944rapid cytoskeleton binding 0.17776604rapid exchange 0.065270099ras-related domain 0.144710192ras-related genes 0.121699341ras-related gtpase superfamily 0.152623579ras-related gtpase superfamily 0.152623579ras-related gtpases 0.21060997ras-related gtpases 0.21060997ras-related gtp-binding 0.214517688ras-related gtp-binding 0.214517688ras-related gtp-binding protein cdna 0.166546364ras-related gtp-binding protein cdna 0.166546364ras-related gtp-binding proteins 0.185737658ras-related gtp-binding proteins 0.185737658ras-related gtp-binding region 0.211930514ras-related gtp-binding region 0.211930514ras-related nuclear protein 0.048066045ras-related proteins 0.072556037ras-related signaling molecules 0.026484256ras-related small gtpases 0.201793136ras-related small gtpases 0.201793136ras-related small gtp-binding proteins 0.181400698ras-related small gtp-binding proteins 0.181400698rat brain cytoskeleton 0.36858052rat myosin heavy chain 0.324665247rat skeletal myosin light chain cdna 0.27277305reciprocal exchange 0.022112095recurrent plasma exchange 0.00202416reduced contractility 0.067294207reduced motility 0.152670623reduced sperm motility 0.112515379reduced sperm tail motility 0.139354199

regulated exchange 0.041752416regulating chromatin structural dynamics 0.036328049regulatory gtpases 0.079588491regulatory myofilament proteins 0.037048702related membrane-associated gtp-binding proteins 0.216083843reorganization downstream 0.147811265repeat contraction 0.007285168replication dynamics 0.059056182required exchange 0.026859364respiratory exchange ratio 0.068483386retinitis pigmentosa gtpase regulator 0.045295535rod domain actin-binding domain 0.15244371rudimentary protrusions 0.240926sarcomere contraction lead 0.198817249segmental exchange 0.022815181sequence exchange events 0.037174324serine base exchange 0.034128898serine exchange 0.049817839serine exchange activity 0.099644309serpin polymerization 0.084887587severe cardiac contractility defect 0.034128898severe wiskott-aldrich syndrome 0.238693077short actin clusters 0.250643497short actin fibers 0.260958265signal exchange 0.058411784signaling gtpase 0.003318933signaling gtpases 0.034128898signal-transducing guanine nucleotide-binding regulatory proteins 0.021896807simple exchange 0.066200608single actin-binding domain 0.193128828single exchange 0.046919128single guanine 0.06405699single nucleotide exchange 0.026963864single-cell motility 0.238693077sister chromatid exchange 0.055079167sister chromatid exchange formation 0.018164973site-directed actin assembly 0.238297549skeletal alpha-actin locus 0.037573419skeletal muscle alpha-actin 0.124705097skeletal muscle bundles 0.111837325skeletal muscle contraction 0.112339897skeletal muscle isometric contractile properties 0.119678002skeletal muscle isometric contractile properties 0.119678002skeletal muscle myosin 0.21096262skeletal muscle tropomyosin 0.234061362

skeletal myosin heavy chain protein levels 0.208247762slow motility band 0.162154616slow-twitch myosin 0.243993619small gtpase 0.182560083small gtpase critical 0.17504848small gtpase regulators 0.126612551small gtpases 0.205578849small gtp-binding protein 0.212748441small gtp-binding protein ral 0.222648627small gtp-binding proteins 0.18186374small guanine nucleotide exchange factors 0.030688636small guanine nucleotide exchange factors 0.030688636small guanine nucleotide-binding proteins 0.061692675small nuclear gtpase 0.09186996small soluble gtpases 0.168083733small stable actin patches 0.236976933smaller wasp 0.204116162smooth muscle actin 0.248215522smooth muscle alpha-actin 0.182191314smooth muscle alpha-actin transcripts 0.155043904smooth muscle aortic alpha-actin 0.139195213smooth muscle cell alpha-actin 0.144354479smooth muscle contractile genes 0.088136814smooth muscle contraction 0.168898976smooth muscle myosin 0.24300227smooth muscle myosin phosphatase 0.222756221sodium-independent exchange 0.026719583sodium-independent organic ion exchange system 0.044247666soluble phosphoinositides 0.063439492spatiotemporal dynamics 0.175352469specific cytoskeletal rearrangements 0.166034179specific exchange activity 0.113996271specific gefs 0.234061362specific guanine nucleotide dissociation stimulators 0.062801113specific guanine nucleotide exchange factor 0.088058117specific guanine nucleotide exchange factors 0.030594276specific guanine nucleotide exchange factors 0.030594276specific microfilament attachment sites 0.072222003specific nucleotide exchange activity 0.079604764specific phosphoinositides direct actin polymerization 0.23349628specific phosphoinositides direct actin polymerization 0.23349628sperm motility 0.139317179spindle dynamics 0.035535651splicing factor dynamics 0.045276727spontaneous contraction 0.080969933spontaneous polymerization 0.100886586

spontaneous sister chromatid exchange 0.039106302stereocilia bundles 0.089763064stimulating guanine nucleotide exchange 0.030751422stimulating guanine nucleotide exchange 0.030751422stimulatory guanine nucleotide-binding protein 0.071629988stimulatory guanine nucleotide-binding regulatory protein 0.027118384strand exchange 0.022596102strand exchange events 0.037285836striated muscle contraction 0.150153295structural dynamics 0.198533569subcortical actin cytoskeleton 0.264552118subcortical actin cytoskeleton 0.264552118subfragment cdna probes 0.234061362surface motility 0.179839068systolic contractile depression 0.126067782systolic contraction 0.040631865t-cell motility 0.03458714telomere dynamics 0.016774515telomere sister chromatid exchange 0.00586682tendon fiber bundles 0.121593543terminal actin subunit 0.410496664thin alveolar walls 0.005352818thin ascending loop 0.065104924thin axonal neurites 0.133653581thin axonal processes 0.138395817thin collagen fibrils 0.013747004thin corpus callosum 0.23260831thin cortical plates 0.159327446thin cortices 0.135520711thin descending limbs 0.07512707thin filament 0.129279978thin filament protein actin 0.251911362thin filament protein actin 0.251911362thin filaments 0.133360445thin hair 0.01137005thin layer 0.141517951thin layer chromatography 0.133716288thin limbs 0.075434295thin long actin filaments 0.234061362thin long bones 0.032164099thin myelinated nerve fibers 0.232131032thin nose 0.194066967thin posterior wall 0.234061362thin ragged coats 0.169412997thin retinas 0.007240191thin rods 0.035606372

thin sections 0.217110897thin skin 0.040706068thin stria vascularis 0.085637079thin superficial cortical layers ii 0.129873715thin ventricles 0.118613296thin ventricular myocardium 0.028153131thin ventricular walls 0.055707955thiol exchange reactions 0.094709513thiol-disulfide exchange 0.053604409total cellular f-actin content 0.262093971total progressive motility 0.138634257total serine exchange activity 0.10377024trachea cell motility 0.046516181tropomyosin isoforms 0.205020774tropomyosin molecule 0.162594797tubulin gtpase activity 0.149280949tubulin polymerization 0.036325261tumor cell motility 0.009821389tumor motility 0.005749414type ii fast myosin 0.250811225typical accessory atrioventricular bundles 0.040187971typical thin cartilage collagen fibrils 0.011447699ubiquitin polymerization 0.003768356unequal sister chromatid exchange 0.053365064uterine contraction 0.073112955vascular smooth muscle contractility 0.142426721ventricular essential myosin light chain 0.157343403vertebrate actins 0.038777733virus-induced cell motility 0.033912699visceral motility 0.14481125vitro actin polymerization 0.226158786vitro contraction patterns 0.047963665vitro gtpase assay 0.12629793vitro motility 0.136193893vitro motility assay 0.108160621volume contraction 0.125055027wasp carboxy-terminal domain 0.228419884wasp deficiency 0.156448706wasp dependent 0.204827569wasp expression 0.172934047wasp genotype 0.131445884wasp mrna 0.198186421wasp mutations 0.078300753wasp protein 0.227033212wasp proteins 0.210169226wiskott-aldrich protein 0.224224848

wiskott-aldrich syndrome 0.053733141wiskott-aldrich syndrome map 0.046845408wiskott-aldrich syndrome maps 0.054074058wiskott-aldrich syndrome protein 0.081353558xy exchange 0.049804158

Table S21. WriteWords-generated word frequency scores from Textrous!-extracted noun-phrases from differential protein expression data from T1R3KO hippocampus. For each word identified from the noun-phrase list (Table S19) its occurrence frequency score is reported.

Word Frequency Scorecortical 161neurons 141synaptic 136cerebellar 131actin 119neuronal 115cell 111cortex 109postsynaptic 108axonal 105neurologic 100rat 82endocytic 76purkinje 69endosomal 66membrane 65synapses 62plasticity 57integral 56human 55cytoskeleton 55axons 55recycling 45synapse 43pyramidal 41gabaergic 39cerebellum 38cells 38protein 37pdz 36motor 35primary 32hippocampal 32cultured 31endocytosis 30fiber 29cerebral 29receptors 28hippocampus 28adult 28

normal 26mouse 26formation 26projection 25clustering 25vesicle 24olfactory 24mossy 24layer 23granule 23excitatory 23bulb 23library 22severe 21nmda 21migration 21ampa 21late 20early 20fibers 19connections 19activity 19vesicles 17transport 17proteins 17mature 17inhibitory 17domain 17brain 17pathway 16membranes 16abnormal 16receptor 15nmdar 15developing 15term 14onset 14mediated 14layers 14impaired 14currents 14ataxia 14terminal 13structures 13multiple 13expression 13

embryonic 13cdna 13transmission 12phenotype 12organization 12neuron 12frontal 12contractures 12compartment 12short 11progressive 11long 11inhibition 11filaments 11disorder 11adrenal 11system 10small 10neocortex 10loss 10filament 10development 10cultures 10complex 10central 10assembly 10adhesion 10vacuole 9trafficking 9tissue 9symptoms 9sensory 9mild 9marker 9ii 9glutamate 9functional 9defects 9cytoskeletal 9type 8transmitter 8thalamic 8surface 8specific 8sorting 8presynaptic 8

markers 8localization 8interneurons 8immunologic 8density 8component 8ataxic 8unmyelinated 7tubular 7terminals 7signs 7release 7region 7postnatal 7plasma 7networks 7n 7machinery 7increased 7granular 7glutamatergic 7fractions 7fast 7compartments 7abnormalities 7visual 6tract 6targeting 6syndrome 6survival 6stress 6spine 6retinal 6proximal 6paraneoplastic 6number 6motif 6ligand 6glycoprotein 6fraction 6enhanced 6endosomes 6dysfunction 6dynamin 6distal 6disorders 6

dendritic 6defective 6death 6congenital 6c 6bundles 6tracts 5thin 5tensin 5temporal 5swellings 5structural 5stimulation 5sg 5rodent 5retrograde 5responses 5rapid 5projections 5processes 5photoreceptor 5neural 5network 5molecules 5methyl 5mapping 5major 5macrocephaly 5lines 5lateral 5impairment 5guidance 5glycoproteins 5events 5ectopic 5dissociated 5dendrites 5decreased 5d 5current 5clusters 5calcium 5bodies 5atrophy 5aspartate 5activation 5

aberrant 5upper 4tubules 4sympathetic 4spontaneous 4spinal 4sites 4samples 4pure 4proper 4precursors 4potentials 4postmortem 4polymerization 4neurotransmission 4neuropathy 4mutant 4lipofuscinosis 4lineage 4kidney 4ipsilateral 4involvement 4intermediate 4infantile 4immune 4hair 4ganglion 4frequency 4form 4fetal 4entorhinal 4endogenous 4differentiation 4deficits 4deep 4containing 4clathrin 4ceroid 4cellular 4binding 4basal 4areas 4area 4apical 4afferent 4ventral 3

subunit 3subcortical 3strength 3specialized 3somatosensory 3soluble 3site 3signaling 3signal 3reorganization 3regulation 3reduced 3properties 3potentiation 3potential 3phosphorylation 3peripheral 3pathways 3parallel 3nucleus 3nuclei 3neuromuscular 3nerve 3mutation 3muscle 3murine 3mrna 3molecule 3mitochondrial 3miniature 3microfilaments 3malformation 3joint 3isolated 3intracellular 3individual 3hypoplasia 3high 3gaba 3fusion 3functionally 3forebrain 3filamentous 3external 3erythrocyte 3endosome 3

dynamics 3dominant 3domains 3distribution 3disorganized 3diseases 3densities 3degeneration 3defect 3cytoskeletons 3conserved 3coated 3cns 3cholinergic 3chicken 3characteristic 3channels 3channel 3bundle 3bovine 3autosomal 3architecture 3apparatus 3anterograde 3altered 3zone 2xenopus 2volume 2vascular 2variant 2vacuoles 2unilateral 2types 2transmembrane 2transferrin 2traffic 2toxicity 2tissues 2target 2syndromes 2sustained 2supply 2superior 2superficial 2subtype 2subfamily 2

studies 2staining 2stable 2spines 2spectrum 2spectrin 2skeleton 2single 2sensorimotor 2segments 2sections 2scaffolding 2root 2role 2relative 2regulatory 2rearrangement 2process 2problems 2principal 2precursor 2potassium 2positioning 2populations 2polyneuropathy 2plate 2phenotypes 2phagocytic 2peroxisomal 2patient 2parvalbumin 2part 2output 2outgrowth 2outer 2origin 2organelles 2observed 2nicotinic 2nervous 2neonatal 2native 2myelinated 2myasthenic 2morphogenesis 2modification 2

microtubule 2microsomal 2mechanisms 2mechanism 2maturation 2mass 2mammalian 2lysosomal 2lower 2localized 2local 2lipid 2libraries 2level 2junctions 2isoforms 2isoform 2ion 2internal 2injury 2independent 2immature 2highly 2golgi 2glycinergic 2general 2forms 2focal 2flow 2fasciculation 2familial 2f 2extracts 2examination 2evoked 2enzyme 2enlargement 2endothelial 2disassembly 2direct 2diffuse 2differentiated 2deletion 2delayed 2culture 2crossing 2

critical 2corticospinal 2control 2condition 2concentration 2components 2complexes 2common 2collecting 2clear 2cholesterol 2chemical 2characteristics 2cervical 2cataract 2cardiac 2canine 2cancer 2broad 2branching 2branched 2boutons 2blockade 2basement 2axon 2avian 2autophagic 2asymmetric 2associated 2anterior 2actomyosin 2activating 2action 2accessory 2a 2yeast 1wiskott 1vocal 1vesicular 1vertebrate 1vertebral 1ventricular 1venous 1variable 1uptake 1unwanted 1

unrelated 1unique 1undifferentiated 1ultrastructure 1tyrosine 1typical 1tremor 1trees 1transporter 1transportation 1translation 1transients 1transient 1transfer 1tracing 1total 1tonic 1tlr 1tight 1thoracic 1thickness 1terminally 1tegmental 1targets 1tagged 1synchrony 1synchronous 1synaptosomes 1swelling 1superfamily 1sumoylation 1steroids 1status 1stability 1spiral 1spiny 1spiking 1spikes 1specificity 1specialization 1sort 1somatic 1smooth 1skeletal 1side 1show 1

shortening 1shapes 1shape 1severity 1service 1sequestration 1sequences 1sequence 1septal 1sensor 1sensitive 1senses 1selective 1segregation 1secondary 1screening 1scaffolds 1scaffold 1route 1roles 1ribosome 1retrieval 1reticular 1retention 1results 1response 1respiratory 1residual 1repetitive 1renal 1regulated 1regression 1regions 1regeneration 1reeler 1recovery 1reciprocal 1recessive 1reception 1raft 1radial 1rabbit 1punctate 1pulmonary 1proteolytic 1proteoglycans 1

protection 1proteases 1proprioceptive 1promoter 1proliferation 1project 1processing 1probability 1priming 1presentation 1preparations 1premature 1predominant 1posterior 1pool 1polypeptide 1polarized 1polarity 1point 1podocyte 1plates 1platelet 1piriform 1pigment 1picture 1physiology 1physiologic 1phosphoprotein 1phosphoinositides 1phenotypic 1phage 1permanent 1period 1perinatal 1pediatric 1pathology 1patches 1parts 1partial 1parietal 1palsy 1ovine 1ovarian 1outcome 1osteoclast 1organized 1

organ 1open 1opacities 1onion 1oculomotor 1ocular 1occipital 1nuclear 1norepinephrine 1nk 1neuropil 1neuropathies 1neurogenesis 1neurofilament 1neurites 1nephrons 1nephrogenic 1neoplasia 1nasal 1myopathy 1myoclonus 1mutational 1mutants 1multivesicular 1movements 1movement 1motion 1motifs 1monosynaptic 1monomeric 1monomer 1module 1modulation 1modulating 1modified 1moderate 1modeling 1mitral 1microtubules 1microsomes 1microglia 1members 1member 1melanoma 1medium 1maximum 1

matter 1mating 1material 1map 1manifestations 1malformations 1maintenance 1main 1macrophage 1macromolecular 1lumbar 1low 1lissencephaly 1lipoprotein 1linker 1lineages 1like 1lens 1lengthening 1length 1learning 1lasting 1lamprey 1laminar 1laminae 1lamina 1kinetics 1keratin 1kainate 1juvenile 1junction 1irregular 1iron 1invagination 1intrinsic 1interphase 1interneuron 1interface 1interactions 1interaction 1interacting 1integrity 1integrin 1integration 1insoluble 1inhibitors 1

infusion 1inferior 1induction 1induced 1increases 1inappropriate 1improvement 1immunological 1immunoglobulin 1illness 1identity 1hypoplastic 1hypofunction 1hrs 1host 1homologous 1homogeneous 1higher 1heterotopic 1hepatocyte 1hemispheres 1hearing 1healthy 1guide 1gtpase 1growth 1group 1gray 1gpi 1glycine 1giant 1genome 1gait 1future 1functions 1frozen 1fragmentation 1fragment 1follicle 1fold 1flat 1fire 1findings 1field 1factors 1facial 1

extraordinary 1extension 1expressed 1experiments 1expansion 1exocytosis 1excessive 1exceptional 1event 1evaluation 1erythroid 1errors 1epithelium 1epithelial 1enzymes 1enteric 1enlarged 1elongation 1elimination 1elevated 1elements 1element 1electrophysiologic 1electrical 1electric 1elastic 1edema 1dystrophic 1dysplasias 1dysarthria 1dynamic 1due 1ducts 1duct 1drug 1dorsolateral 1dopaminergic 1dopamine 1dominance 1diversity 1disturbed 1disturbances 1distress 1disorganization 1disease 1discrete 1

directed 1dilated 1digital 1diameters 1developmental 1depressing 1dentate 1delivery 1defined 1deficit 1decrease 1decline 1damage 1cytosolic 1cytosol 1cytoplasm 1cysts 1cycle 1crude 1crosslinking 1corticostriatal 1cortices 1correction 1coordination 1contraction 1contractile 1content 1contacts 1contacting 1contact 1constitutive 1consolidation 1conduction 1conditions 1concomitant 1complications 1complete 1competitive 1columns 1column 1codes 1cobblestone 1cluster 1clefts 1classical 1classic 1

cingulate 1chromatin 1chloride 1childhood 1center 1caudate 1cathepsin 1cataracts 1carrier 1carboxy 1capillary 1capillaries 1cancers 1brush 1branch 1brainstem 1bound 1border 1body 1blood 1blocks 1blocking 1blindness 1biogenesis 1bilateral 1bidirectional 1benefit 1beginning 1balance 1autism 1atp 1associative 1assay 1artificial 1arrangement 1apparent 1antigens 1antibody 1antagonist 1anomalous 1anlage 1anchored 1anchor 1amacrine 1alignment 1algorithms 1

aldrich 1albumin 1airway 1agonist 1aggregate 1advanced 1adhesions 1adaptors 1adaptive 1active 1activated 1actions 1acidic 1accounts 1absolute 1abnormality 1

Table S22. WriteWords-generated word frequency scores from Textrous!-extracted noun-phrases from differential protein expression data from T1R3KO cortex. For each word identified from the noun-phrase list (Table S21) its occurrence frequency score is reported.

Word Frequency Scoreactin 220exchange 120cytoskeletal 114myosin 107binding 90gtpase 78protein 63guanine 62motility 61nucleotide 59contractile 57cytoskeleton 53gtp 50dynamics 48domain 46muscle 44proteins 41thin 40activity 37f 36bundles 36contraction 33chain 32nonmuscle 30cell 29related 27terminal 25ras 25contractility 25cardiac 25polymerization 24n 24heavy 23wasp 22factor 21filament 20organization 18small 17reorganization 17filaments 17

skeletal 16membrane 16cortical 16alpha 16activating 16specific 15human 14light 13gtpases 13filopodia 13smooth 12conserved 12genes 11cellular 11wiskott 10regulatory 10rapamycin 10protrusion 10phosphoinositides 10network 10ii 10aldrich 10structures 9region 9protrusions 9microtubule 9isometric 9syndrome 8monomeric 8microfilament 8intracellular 8increased 8fibers 8cdna 8c 8assembly 8actomyosin 8tropomyosin 7sister 7myosins 7lamellipodia 7hair 7formation 7filamentous 7factors 7chromatid 7

actins 7tail 6site 6normal 6microvillus 6long 6fiber 6decreased 6abnormal 6vitro 5ventricular 5single 5signaling 5serine 5ring 5plasma 5performance 5myocardial 5mouse 5mammalian 5kinase 5isoform 5intermediate 5impaired 5highly 5force 5enhanced 5domains 5defects 5crosslinking 5chains 5bind 5beta 5basal 5axonal 5vi 4superfamily 4stress 4staining 4sperm 4sites 4sequence 4responses 4regulators 4reduced 4reaction 4

rapid 4radixin 4phosphatase 4nuclear 4motifs 4major 4low 4isoforms 4intrinsic 4heterotrimeric 4head 4epithelial 4endothelial 4dissociation 4dependent 4cycle 4core 4collagen 4central 4cells 4assay 4activities 4activation 4aberrant 4va 3total 3system 3strength 3strand 3spontaneous 3rod 3results 3regulator 3regulation 3rearrangement 3rat 3purified 3properties 3polarized 3podocyte 3osteoclast 3non 3neuronal 3networks 3myopathy 3myofilament 3

myofibril 3motor 3motif 3molecules 3molecule 3matrix 3locus 3limbs 3kinetics 3iib 3iia 3golgi 3gdp 3gastrointestinal 3functions 3frap 3fast 3extracts 3events 3elements 3dynamic 3direct 3dendritic 3deficiency 3defect 3cytoplasmic 3content 3class 3chromatin 3attachment 3associated 3apparatus 3anion 3altered 3abnormalities 3walls 2vimentin 2viia 2vascular 2typical 2tumor 2tubulin 2tissues 2thiol 2telomere 2systolic 2

surface 2subunits 2subunit 2substrates 2substrate 2subfragment 2subcortical 2studies 2structural 2storage 2stimulatory 2stimulators 2stimulator 2stimulating 2spectrin 2soluble 2sodium 2slow 2signature 2signal 2short 2severe 2sequences 2response 2repeat 2remodeling 2release 2rearrangements 2ral 2rabbit 2progressive 2primary 2potential 2porcine 2platelet 2phenotype 2parallel 2outer 2neuron 2neural 2nerve 2myofilaments 2myocyte 2mutant 2mrna 2modeling 2

mitotic 2mitochondrial 2microspikes 2microfilaments 2mechanism 2mature 2localization 2local 2linker 2levels 2left 2layer 2keratin 2internal 2insoluble 2inhibitory 2inhibitor 2independent 2iiia 2iii 2hydrogen 2homology 2homologous 2histone 2higher 2heart 2gut 2growth 2genotype 2gefs 2fundamental 2fibrils 2ezrin 2eukaryotic 2essential 2erythroid 2erythrocyte 2endogenous 2embryonic 2elongation 2effector 2dna 2disorganized 2disassembly 2descending 2defective 2

critical 2conformational 2cone 2complex 2complete 2cofactor 2ciliary 2chromosomal 2chromatography 2characteristic 2catalytic 2bundle 2brain 2base 2bands 2architecture 2aortic 2alkali 2adaptor 2active 2activators 2acid 2xy 1wound 1work 1wall 1volume 1voltage 1visceral 1virus 1vestibular 1vertebrate 1ventricles 1vascularis 1uterine 1unequal 1ubiquitin 1types 1type 1twitch 1triphosphate 1treatment 1transport 1transmembrane 1transition 1transfusion 1

transducing 1transcripts 1trafficking 1tract 1trachea 1tissue 1tips 1tendon 1technique 1targets 1talin 1tails 1t 1systems 1synuclein 1synthesis 1syncytiotrophoblast 1suppressor 1superficial 1striated 1stria 1stimulus 1stereocilia 1stable 1stability 1splicing 1spindle 1specificity 1specialized 1spatiotemporal 1smaller 1skin 1skeleton 1simple 1serpin 1sequestration 1segmental 1sections 1scaffolds 1sarcomere 1rudimentary 1rods 1retrograde 1retinitis 1retinas 1restructuring 1

restriction 1respiratory 1residues 1reserve 1required 1replication 1regulating 1regulated 1recurrent 1recruitment 1recombinant 1reciprocal 1receptors 1reactions 1ratio 1rates 1rate 1random 1ragged 1rag 1punctate 1pulmonary 1promoter 1projection 1production 1product 1processes 1process 1probes 1principal 1presynaptic 1preparations 1predominant 1postsynaptic 1postprandial 1posterior 1positive 1positions 1poorly 1poor 1polymers 1polarization 1polarity 1points 1plates 1placenta 1

pigmentosa 1physiologic 1photoreceptor 1phosphorylation 1peripheral 1patterns 1pathways 1pathway 1patches 1partner 1palmitoylation 1oxygen 1organic 1number 1nucleotides 1nucleocytoplasmic 1novo 1nose 1neurites 1necrosis 1natural 1myoepithelial 1myocardium 1myelinated 1mutations 1mutational 1mutated 1motion 1monomer 1module 1modified 1missense 1methylation 1membranes 1melanin 1mechanisms 1maximum 1maximal 1maxillary 1marker 1maps 1map 1machinery 1lower 1loop 1localized 1

listeria 1level 1leukocyte 1length 1lead 1layers 1latent 1labeled 1l 1kinetochore 1junction 1jejunal 1ix 1isotope 1isolated 1irreversible 1ion 1interphase 1intermediary 1intergenic 1interfaces 1interface 1interactions 1inhibited 1induced 1inclusions 1inclusion 1impulse 1impairment 1immunosuppressive 1immune 1ic 1hydrolyzing 1host 1homologs 1homolog 1hepatocyte 1hepatic 1helical 1hearts 1guanosine 1greater 1globular 1generation 1gels 1gastric 1

gas 1gallbladder 1functional 1frontal 1frequencies 1framework 1fragments 1fraction 1fos 1form 1forces 1fold 1flanking 1fibrous 1fibroblast 1fetomaternal 1expression 1experiments 1exon 1excessive 1excess 1event 1ester 1epigenetic 1enzyme 1envelope 1endosome 1endosomal 1emerging 1element 1edge 1dysfunction 1dynein 1dynamin 1drug 1downstream 1disulfide 1disruption 1disorders 1disease 1disabled 1directed 1diphosphate 1dimerization 1dimer 1dictyostelium 1

detrusor 1derivative 1depression 1depressed 1density 1dense 1deaminase 1de 1dc 1cytosolic 1cytoskeletons 1cultured 1cortices 1corpus 1coordination 1controls 1continuous 1constriction 1consensus 1components 1component 1cochlear 1coats 1clusters 1clones 1clinically 1cilium 1cilia 1chromosome 1chondroitin 1cholesteryl 1chicken 1channel 1change 1cenocepacia 1caveolae 1cation 1cascades 1cartilage 1carboxy 1cancer 1canalicular 1calyx 1callosum 1calcium 1brush 1

bovine 1bound 1border 1bones 1biosynthesis 1biliary 1behaviors 1band 1axon 1autonomous 1autocrine 1atypical 1attachments 1atrioventricular 1associates 1ascending 1arrangement 1apparent 1aplysia 1apical 1aorta 1antiviral 1antigen 1anterior 1ankyrin 1ancient 1anchorage 1anchor 1analysis 1analogs 1analog 1amino 1alveolar 1alternative 1allosteric 1adrenergic 1administration 1activated 1actinin 1acetylated 1accessory 1accelerating 1ability 1