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Week 11 August 20, 2016 Digested Globin pgRNA using BstX1 and Xho1 Overnight 11ul Water 1ul Template 5ul xHo1 1ul BstX1 2 ul 3.1 Buffer August 21, 2016 Globin pgRNA Gel Lane 1 – Ladder Lane 2 - Empty Lane 3 – Negative Control Lane 4 – Empty Lane 5 – pgRNA Gibson Product Analysis The negative control ran where it should have but the Globin pgRNA did not show up at all. We will run again in gel with new Gibson product.
