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What is SV40 T-antigen?
What does it do?
Presented by 李柏毅Genome Engineering Laboratory,
Institute of Bioinformatics,National Yang-Ming University
2003/12/12
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Simian Virus 40
Hosts: monkeys and mice DNA virus with 5,227 base pairs
Origin
Early mRNAs Late mRNAs
5’ 5’
SV40
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SV40 T-antigen
AUG UUA UUAEarly pre-mRNA
Large T mRNA
Small T mRNA
AAAn
AAAn
Large T 94kd proteinSmall T 17kd protein
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Large T-antigen
Delete 1-126 a.a. Delete 136-C terminus Addition 127-132 a.a to
β-galactosidase and pyruvate kinase
Delete 127-132 a.a.
Immunofluorescence microscopy
Daniel Kalderon, Bruce L. Roberts, Alan E. Smith, A Short Amino Acid Sequence Able to Specify Nuclear Location, Cell 39:499-509, 1984
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Tumor Virus
SV40 Large T
Ad5 E1A 13S
Ad5 E1A 12S
Ad5 E1B 55K
Rb-binding P53-binding
Release transcription factor for Rb
Protein binding sites