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Let’s think about bacteria...
Let’s think about bacteria...
Synthetic biology
Let’s talk about Biology’s Central
Dogma
DNA mRNA Protein Function
Vancouver HalifaxTransitStation
Function
Function
DNA mRNA Protein Function
General Rule:
Let’s break a rule
DNA mRNA Protein FunctionAll DNA have function
sensing
tln initiation stop signs
Images courtesy of Parts Registry (parts.mit.edu)
protein coding
Think of these as DNA parts
Images courtesy of Parts Registry (parts.mit.edu)
Think of these as DNA parts
Images courtesy of Parts Registry (parts.mit.edu)
We can put these parts together, and stick them into bacteria
Parts of DNA
Escherichia coli
arabinose-sensing
promoter
Aequorea victoria GFP & variants
Vibrio fischeri
terminator
+ + = ??
Images courtesy of Roger Tsien Lab (http://www.tsienlab.ucsd.edu/Images.htm)
Cutting & Pasting DNA
Reading DNAGenetic
Engineering
Let’s standardized these parts
Images courtesy of Parts Registry (parts.mit.edu)
BBa_J23100
BBa_B0034
BBa_K145015
BBa_B0014
Cutting & Pasting DNA
Reading DNA
Standardization
Abstraction & DesignSynthetic Biology
Genetic Engineering
Writing DNA
Assembling DNA is like programming!
Genetic Programs
this is the platform
so-so??
awesome!
so-so??
Genetic Programs
awesome!
so-so??
really exciting!
Cutting & Pasting DNA
Reading DNA
Standardization
Abstraction & Design
Platform
Synthetic Biology
Genetic Engineering
Writing DNA
Synthetic biology makes biotechnology
accessible
Standardization enables development
Biochemical Bags of Blood
+ + + +
Oxygen transport
Immunologically invisible chassis
Protein production controller
Genetic self-destruct
Freeze-drying
Images courtesy of UC Berkeley’s 2007 iGEM Team (http://parts.mit.edu/igem07/index.php/Berkeley_UC)
Video taken from Stricker et. al.; Nature 2008 vol. 456, 516-519; doi:10.1038/nature07389
Genetic Oscillators
Video taken from Stricker et. al.; Nature 2008 vol. 456, 516-519; doi:10.1038/nature07389
Genetic Oscillators
Genetic programming using Synthetic Biology
can impact the WORLD!
“Gas-Guzzling” bacteria can consume
greenhouse gases
We can do biological computation
We can do biological computation
12
3
AB
C
Friedland et. al., Science 2009
We can do biological computation
12
3
AB
C
Friedland et. al., Science 2009
001011001001
Sometime coming...?
Synthetic biology is exciting!
Synthetic biology is exciting!♥
interdisciplinary
fundamentally powerful
Synthetic biology can change the way we
think about life science education
Mens et Manus ::: Mind and Hand
2009!
2009!
Thank
s Dr.
Laga
lly!!!
complete research
experiencefunding
experiments
writeup
idea
Analog biosensor with thresholds for outputs
Applications in biomedical diagnostics,
therapeutics & environmental sensing
increasing arabinose
idea
complete research
experience
idea
funding
experiments
writeup
UBC Teaching & Learning Enhancement Fund
funding
complete research
experience
experiments
writeup funding
idea
experiments
complete research
experience
experiments
writeup
idea
funding
writeup
complete research
experience
experiments
writeup
idea
funding
We can finally move into an era of applied & problem-based learning
www.igem.orgwww.ubcigem.com
