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Where Industry meets Science
Station de Biologie Marine ConcarneauAugust 28th and 29th 2012
“Ifremer Technology Transfer Strategy”
Patrick Durand – Head of Biotechnology and Marine Resources Unit (RBE/BRM IFREMER Atlantic Center)[email protected]
The French Research Institute for Exploitation of the Sea
IFREMER’S MISSIONS
> Finalized research in the prospect of answering t o social expectations(climate change effects, marine biodiversity, pollu tions prevention, seafood quality…). The results are scientific knowledge and technological innovations, but also ocean observation and exploration systems. The partnership is The partnership is public, private or both.public, private or both.
> Oceans seas and coastal waters’ monitoring, in su pport with public policyfor the environment and its resources. In the form of notices or studies reports, the results provide the information about the environment state or the evaluation of a resource and permit the expertise o n sea-related questions.
�Development, management and open access of large re search infrastructures – fleet, calculation means, data centers, experiment al facilities– which are made available to the whole National and European scientific community, as well as to private partners.
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IFREMER’S SCIENTIFIC EXPERTISE
> Monitoring, use and promotion of coastal seas
> Monitoring and optimizing aquaculture products
> Promoting scientific advices on fisheries resources for a sustainable fishing
> Exploring the seafloor and the ocean biodiversity
> Forecasting ocean circulation, marine
ecosystems evolution
> Managing vessels and underwater vehicles for the whole scientific community
KEY FIGURES
> An annual budget of around 235 million euros
> Personnel : Ifremer 1,500 ; Genavir (ship owner) 375
> 5 centres (Brest, Manche/mer du Nord,
Mediterranée, Nantes, Tahiti)
> 26 locations along the entire coastline of
metropolitan France and in French overseas regions
> 8 research vessels (4 of them ocean class),
1 manned submarine, 1 Remotely-Operated Vehicle for deep sea exploration (- 6000 m), 2 AUVs
> A wide array of experimental facilities
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Organization chart :
Voir avec NS pour récupèrer organigramme ifremer (av ec DDVPE
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P1: Vision of physic oceanographers and geologists: P1: Vision of physic oceanographers and geologists: P1: Vision of physic oceanographers and geologists: P1: Vision of physic oceanographers and geologists: New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,New Frontiers, 3D Vision,
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vision of biological oceanographers : ocean of ocean of
biodiversity and a tremendous reservoir of genesbiodiversity and a tremendous reservoir of genes
Unit BRM :Biotechnologies
& Marine Resources
www.ifremer.fr/francais/org/bm.htm
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Biotechnologies & Marine Resources Unit is organised around :
A permanent staff of 50 people :
�30 researchers (engineers and PhD, 8 HDR)
�16 technicians
�4 administrative support
& a non permanent staff of 30 :
Trainees, PHD, Post-doctoral
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Three laboratories :
� 2 located in Nantes (Pays de la Loire Region)
� 1 bilocalized Nantes/Brest (Brittany Region)
Objectives :
“Develop enhanced value and use of biological resources through biotechnologies and bio-prospection”
Priorities of the 4-years contract and one of the 10 structuring orientations for 2020 Ifremer Strategic Plan
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Main objectives :
to organise and federate competences:
� To promote seafood development through research projects on food processing , in relation with food quality and safety
� To develop knowledge and tools for marine species identification
� To contribute to fishery by-product enhancement
� To bring to administration and professional organisations, scientific, technological and regulation knowledge through bibliographic review(Bibliomer)
1 Laboratory Science & Technology of Marine BiomassHead of the laboratory : Jean-Pascal Bergé BRM/STBM
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1 BRM/STBM Laboratory Science & Technology of Marine Bi omass
Interactions process/products Consumers
expectations
Food applications
TraceabilitySafetyRegulation
Resources
- Ingredients- Nutraceutical and functional food
Screening and characterisation of new raw material
By-products
Screening and characterisation of new molecules
Quality check
- Foodstuff
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Develop enhanced value and use of biological resour ces through biotechnologies and bio-prospection
2 Laboratory Biotechnology & Marine MoleculesHead of the laboratory : Sylvia Colliec-Jouault BRM/STBM
Micro algae…
Marine bacteria
Deep-sea hydrothermal vents …
Microbial mats in Polynesian atolls
PHA
Macro algae… EPS
Marine algae
Biopolymers of industrial interest from extreme ecosystem s
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� Glyco-biotechnology & Engineering
Project leader : Sylvia Colliec-Jouault
� Environmental Biotechnology
Project leader : Christelle Simon-Colin
2 BRM/BMM Laboratory Biotechnology & Marine Molecules
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Two complementary teams :
� Ecophysiology
� Molecular Biology
3 Laboratory Physiology and Biotechnology of AlgaeHead of the laboratory : Jean-Paul Cadoret BRM/PBA
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Ecophysiology
Molecular Biology
Metabolism (mineral nutrition)
Irradiance
Interaction of environmental factors
Molecular taxonomy
Cell Factory
BRM/PBA Laboratory Physiology and Biotechnology of Algae
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Equipment:
•Photobioreactors (200mL, 2L, 6L, 120L),Batch, fed batch, continuous cultures,DOE (Design of Experiment) unit,PCR, Q PCR,HPLC,CN analyzer,Ionic chromatography.
BRM/PBA Laboratory Physiology and Biotechnologie of Algae
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our Offers in portfolio
� Marine Products andco- products Security/quality of seafoods, Upgrading-transformation, improvement of agrifood processes
� marine Biotechnology
extraction characterization of innovative marine molecules (biopolymers of microorganisms), bioremediation, biofuels and biodegradable plastics � Aquaculture
Detection system and risk prevention, analysis development of sustainable production systems, genetic selection
� halieuticCampaign for stock assessments, expertise and training in fishery selectivity, simulation software ...
� Underwater interventions
Research vessels, underwater vehicles, deep mapping, seismic studies in marine geosciences, energy resources, ..), recovery of objects
� testing facilities
Towing tanks, hyperbaric chambers, laboratory behavior of materials and metrology
� Coastal and offshoreMonitoring tools and measurement sensors forecasting model of the sea state, environmental impact studies, info systems. environmental GIZC
Potential value in biotechnology
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Transfer strategy
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Our research offers in biotechnologies
Food industryBioprotective bacteria (from seafood products)
Active molecules encapsulationpackaging, cosmetic formulation, adhesive molecules
- polyhydroxyalkanoates (PHA)
Cosmetic, nutraceuticalActive ingredient (marine by-product)
Tissue regeneration, cardiovascular, oncology, oil exploitation, biofilm prevention and fouling (industry et painting), bio-chelation
Bacterial biopolymers :- exopolysaccharides (EPS) + enzymatic processes for EPS modifications
Food industry, oncology (pigments), antimicrobial peptides (veterinary)CO2 sequestration
Microalgae (biomass valorization)
Biomolecular laboratory reagents (replication, DNA reparation)
Thermostable proteins & enzymes
Industrial sectors targetedStudied molecules
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Examples of transfers to industry
License to existing partner
MTA with license option
Spin off 2009
Environment
Pharma/cosmetic. POLYMARIS
Bacterial EPS+
EPS
License to existing partner MP BIOMEDICAL
Thermostable
Enzymes for PCR
Spin off 2008ALGENICSMicroalgal(bioproduction systems
Agrifood
biopreservation
THERAPOL
SEADEV
PROTEUS
Partner/ sector
License to existing partner
MTA with license option 2011
Bacteria coming from seafood matrix
Development intermediary v/v pharma companies / biotech (engineering science and technology)
Fucans from brown seaweeds
Start-up creation in 2004
JEI support 1999
hydrothermal bacterial
Collection
Hydrothermal Enzymes
ContextMolecules/
microorganisms
012345678
0 7 14 21 28
Time (days)
log
(cfu
/g/ g
)
Lm+ CdV41
Lm
Cd V41
4°C
8°C
Listeria monocytogenes growth(red,solid line) or in the présence ofCarnobacterium species LYOFLORATM
FP 18 (red, dotted line). (blue)
Collection of Bacteria coming from seafood matrix ; Utilization in Biopreservation
Transfert : license to existing partner MTA with license option
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Proteus uses two complementary approaches for the d esign of new biocatalysts :- Screening of microbial (gene) diversity - Protein engineering (using directed evolution)
L-Shuffling™
New Biocatalysts
Pool of selected
genes
Phenomics® screening
Microbial BiodiversityEnvironment gene mining
“Nature has more imagination than our dreams”“Nature has more imagination than our dreams”“Nature has more imagination than our dreams”“Nature has more imagination than our dreams”
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The Next Generation Platform for the Biomanufacturing of Recombinant Therapeutics
ALGENICS is a biopharmaceutical companybiopharmaceutical company leveraging a unique microalgae-based
technology to produceproduce recombinantrecombinant therapeuticstherapeutics for animal and human health.
Microalgal cell lines engineered using our platform AlgebiosysTM are available under
licensing agreement for production of glycosylated th erapeutics with preferential
applications in the field of monoclonal antibodies and viral subunits.
Our strategy is also to develop proprietary biotherape utics for human use benefiting
from our technology. Targets include recombinant produ cts with
enhanced biological activities for applications in on cology and virology.
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Food
Health
Energy
Environnement
Industry
MolecularBiology
Biochemistery
EcoPhysiology
BioInformatics
GeneticConservation
BRM/PBA Laboratory Physiology and Biotechnologie of Algae
IMA prefiguration: Institute of MicroAlgae
mixed structure (public private)
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Thank you for your attention
