V o l u m e 1 I s s u e 1

Which compound can be analyzed with an

11-minute method using online sample extraction?

F e a t u r e d A p p l i c a t i o n N o t e :

Additional Application Notes:

Quantitation of Fentanyl and Norfentanyl fromUrine Using On-line High Throughput System

Francois A. Espourteille, Ph. D., Thermo Fisher Scientific

Increasing demand in clinical research laboratories for higher sample throughput has put the emphasis on automated methods and platforms that have the ability to quickly ramp up throughput tomeet demand. The AriaTM TLX-4 system powered by Thermo ScientificTurboFlow technology provides a fast, efficient, and automated online separation technology for the extraction and analysis of fentanyl and its metabolite, norfentanyl.

Rapid Analysis of Opiates from Low Volume Whole Blood Samples by LC-MS/MS Utilizing TurboFlow Methods

Peter Ashton, Alex Allan, Bob Ardrey, Triple A Forensics Ltd.

Shane McDonnell, Sarah Robinson, Thermo Fisher Scientific

The analysis of free- and protein-bound opiate analytes in human wholeblood by LC-MS/MS is routinely done after rigorous sample clean up viasolid phase extraction or liquid-liquid extraction in order to minimize ionsuppression in the ionization source of the mass spectrometer. These clean up steps can be lengthy, laborious and expensive. Here we present a method to quantitatively analyze opiate compounds present in wholeblood utilizing a simple, fast, low-volume extraction procedure followed by online extraction and chromatography coupled with selected reactionmonitoring tandem mass spectrometry.

Yang Shi, Catherine Lafontaine, Chris Esposito,Francois Espourteille, Thermo Fisher Scientific

Current clinical research methods usually involve labor-intensive, time-consuming offline sample preparation. In this study, a sensitive, selective LC-MS/MS method was developed using the Thermo Scientific Aria TLX-1 system powered by TurboFlowTM technology for online sample extractioncoupled with the Thermo Scientific TSQ Vantage triple stage quadrupole mass spectrometer. The results demonstrate the method’s suitability for clinical researchers to measure MN and NMN.

Read or download the full studies on thermo.com/clinicallycurrent

Plasma-free Metanephrine and Normetanephrine Quantitation Using Online Sample Extraction Coupled with Tandem Mass Spectrometry

Maximum roller coaster G-force approaches that of the

Space Shuttle on take-off.

The International Association ofForensic Toxicologists (TIAFT)Geneva, SwitzerlandAugust 23 - 27, 2009

International Association of Therapeutic Monitoring andClinical Toxicology (IATDMCT)Montreal, CanadaOctober 3 - 8, 2009

Society of Forensic Toxicologists (SOFT)Oklahoma City, Oklahoma, U.S. October 18 - 23, 2009

Be sure to visit Thermo Fisher Scientific if you’re attending any of these events:

Recent webinars available on thermo.com/clincallycurrent• Why LC-MS/MS for Forensic Toxicology Screening?• High Throughput in the LC-MS/MS Laboratory

Find out the answer, and why, on thermo.com/clinicallycurrent

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Thermo Fisher Scientific is committed tohelping Clinical Research and ForensicToxicology Laboratories by optimizingworkflows and integrating solutions toachieve unprecedented success.

Our dedicated team of sales professionalsuses a consultative approach to helpyou determine the best solution to meet and exceed your goals.

Whether it’s helping you optimize your LC/MS workflow by using our mul-tiplexing and TurboFlow™ technology,or helping you decide which platform ofour broad range of analytical instrumen-tation – from AA and ICP-MS to GC/MSand LC/MS – is best suited for your lab, we are here for you.

In addition to offering our technical solutions, we can help you with thebusiness side of science. Whether it’s working through an ROI with your CFO or helping you work out details of your service requirements, we arehere to help you.

We are committed to your success andlook forward to working with you.

If you are located outside of the US, please contact +44 1442 233555 oranalyze.uk@thermo.com for help in locating your local sales representative.

How do I improve the productivity of LC-MS/MS assays in my clinical research lab?

The major advantages of using liquid chromatography-mass spectrometry (LC-MS/MS) in clinical research are fast assay development, low cost-per-analysis, high specificity and superior sensitivity. These major advantages are sometimes overshadowed by thelengthy sample preparation associated with traditionalLC-MS/MS methods. Many clinical research labs arenow adopting a new, completely automated method including online sample extraction and multiplexing to dramatically improve productivity of their LC-MS/MS assays.

Thermo Scientific TurboFlow technology is a superioronline sample extraction technique for complex biological matrices such as blood, urine and saliva.This online, automated process combines the principles of diffusion, chemistry and size exclusion toquickly eliminate matrix interferences while capturinganalytes of interest. With this technology, relativelycrude biological samples can be analyzed directlyavoiding interference from even the high levels of proteins, salts and phospholipids in biological matrices which are normally very challenging for mass spectrometry analysis. Labs who have adopted TurboFlow™ technology have seen a reduction in sample preparation by up to 95%.

Thermo Scientific multiplexing technology is unique in that it offers the productivity of up to four separate, parallel liquid chromatography systems to a single massspectrometer. With its proprietary software, each channeloperates independently, so you can run a single method or multiple methods simultaneously. Sample introductionis staggered in such a way that analytes emerge from eachof the channels serially without overlap between channels. This critical feature improves mass spectrometer efficiency, unlike traditional single-channel LC systems whose detectors are idle more than 75% of the time. Labs whohave adopted this proprietary multiplexing technique have seen an increase in sample throughput by up to 4 times.

Save money effortlessly and boost productivity – without compromising data quality or sensitivity.

Learn more about TurboFlow technology and multiplexing at thermo.com/clinicallycurrent

Q

A

pCommercial Corner

Mass Spec in Monthly Payments

Is your lab conserving cash? Are you in need of new equipment?Whether you need a gas chromato-graph, mass spectrometer, centrifugeor one of the hundreds of otherproducts and supplies you use to do your work, our Clinical Research & Forensic Toxicology Sales Specialists will work to meet your financing challenges.

We strive to provide our customerswith class leading financial solu-tions and where possible, we workhard to meet the needs of emergingcompanies and new labs by provid-ing financial support for the acquisi-tion of your scientific equipment.

Companies fitting the following profile may be among thoseawarded credit approval:

• A solid cash position

• Strong existing collaborations

• Promising science

• Experienced management team

Thermo Fisher Financial Services can source a number of different financial solutions which shouldmeet with your requirements. These may vary by country and currency, and range from conven-tional installment credit through finance leasing and tax based “off balance sheet” facilities.

“At Thermo Fisher Scientific we are proud of our mission, which is to

enable customers to make the world a healthier, cleaner, and safer

place. Our lease and finance programs offer our customers a solution

to acquire the equipment they need to make this happen.”

Marc Casper, CEOThermo Fisher Scientific