Date post: | 13-Jan-2017 |
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AdAptA project:
Population size and population origin for
marginal/peripheral populations
• Alfredo García Fernández
• Carlos Lara-Romero
• Javier Morente-López
• Mª Luisa Rubio Teso
• Jose Carmen Soto
Correa
• José M. Iriondo Alegría
• COST ACTION MaP JACA 2014.
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
• High mountain ecosystems, highly endangered by global change.
• For many alpine species, Mediterranean mountains are southern limit of distribution
(glacial refugee).
• Upward/latitudinal migration is not an option.
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
• High mountain ecosystems, highly endangered by global change.
• For many alpine species, Mediterranean mountains are southern limit of distribution
(glacial refugee).
• Upward/latitudina migration is not an option.
• Adaptation as one of the main response against new environmental conditions.
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
• High mountain ecosystems, highly endangered by global change.
• For many alpine species, Mediterranean mountains are southern limit of distribution
(glacial refugee).
• Upward migration is not an option.
• Adaptation as one of the main response against new environmental conditions.
• Introduction of “altitude variable” in the consideration of Central - Periphery populations
e.g. One population in the southern distribution limit but at intermediate altitude could be
considered marginal population?
Local gradient VS Whole distribution.
Silene ciliata Pourret
• Perennial cushion plant.
• Mediterranean mountain plant
• Central System (Central Iberian Peninsula) southern
limit of species distribution.
• Small occupation area. Certain population isolation.
• Inhabit altitude range from 1900 to 2500 m.a.s.l.
o High phenotypic variability
o Population genetic
o Limited dispersal availability
o Demographic fitness
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Not a forest species
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Importance of gene flow and population size in evolutionary potential in marginal populations
FITNESSEvolutive potentialGenetic diversity
Marginal populations
Gene flow between populations of different size.BIG/SMALL
(Lemu & Fischer 2008)
Natural selection
Gene flow between populations CENTRER/PERIPHERY
(Sexton et. al. 2011)
?
?
OBJETIVES
Evaluate the effects of gene-flow between Center-Periphery VS Periphery-Periphery and
population size in the biological performance of individuals from marginal populations of S.
ciliata.
QUESTIONS:
(1) Can be improved the viability of marginal populations by gene flow with pollen from
populations that occur in the environmental optimum conditions of the species?
OR MAYBE
(2) Could be more effective the gene flow with pollen from other marginal populations that are
under similar selective pressures?
AND ALSO
(3) What is the influence of population size in the population viability?
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Definition of centrer and periphery
A critical step for our approach is the definition of center and periphery populations
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Ávila
SegoviaSalamanca
Madrid
• Environmental point of view
instead of geographic
• MAXENT approach with
environmental values that explain
the species distribution.
• Identification of center and
periphery populations
Definition of centrer and periphery
A critical step for our approach is the definition of center and periphery populations
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
GuadarramaGredosBéjar
• Central Big Population
• Peripheric Big Population
• Peripheric Small Population
EXPERIMENT 1:
1. In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-
populations).
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Planteamiento Diseño PreguntasIntroducción
Periphery Big from other mountain range
Periphery Small A
Central Big A
Periphery Big A
• Each periphery population received pollen from itself, from the central of its same mountain range and from other peripheral of the same or bigger population size.
Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Periphery effects
• Plant cuttings from 20 mother plants in each population
Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations).
• Manual crossings.
• Seed sowing in situ in periphery populations:• 3 types of crossings.• Control of intrapopulation variability. • Block design to avoid environmental heterogeneity
• Production of clonal families from cuttings.
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
180 mother plants!
14400 cuttings!!!
18000 crosses!!!
Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations).
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
24000 SEEDS !!!!!!
Béjar
Guadarrama
Gredos
¿?
¿?
¿?
Experiment 2: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Full effects.
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Periphery Small
Central Big
Periphery Big
Periphery Small
Central Big
Periphery Big
Periphery Small
Central Big
Periphery Big
Experiment 3: Transcriptome comparisons during the seedling stage
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Periphery population
Central population
Seed collection Greenhouse sowing ARN extraction and sequencing
Plant ARN after
drought treatment
Plant ARN control
Some ideas…
• Experiment 1: Common garden approach, Periphery effects:
Sowing design.
• Experiment 2: Common garden approach, Full effects:
Following the same design as experiment 1, > 50,000 seeds.
• Experiment 3: Transcriptome approach:
Experience in NGS?
Drought treatment?
Pool individuals
Ideas & ObjetivesDesigns &
ExperimentsQueriesIntroduction
Thanks for your attention
AdAptA project:
Population size and population origin for
marginal/peripheral populations
• Alfredo García Fernández
• Carlos Lara-Romero
• Javier Morente-López
• Mª Luisa Rubio Teso
• Jose Carmen Soto
Correa
• José M. Iriondo Alegría
• COST ACTION MaP JACA 2014.