1
World Renowned HPLC MediaReinvented for UHPLC!
New Luna Omega 1.6 µm • Staggering UHPLC Efficiencies and Performance
• Extreme Ruggedness and Mechanical Strength
• Perfect Complement to Kinetex® Core-Shell Technology
NEWPolar C18 and C18 Selectivities
2
Enhanced with 20 Years of Technology,
Innovation, and Experience
One of the world’s leading HPLC brands, now enhanced for incredible UHPLC performance! Luna Omega 1.6 µm UHPLC columns culminate 20 years of technological prowess, advancements, and innovation from Phenomenex!
With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, Luna Omega columns will take your UHPLC
experience to a new level.
Luna® Omega UHPLC columns will boost your UHPLC instrumentation!
3
Cutting Edge UHPLC ..................................................................... p. 4
Astounding Performance .............................................................. p. 5
Separation Muscle ......................................................................... p. 6
Inert Foundation ............................................................................. p. 7
Excellent Reproducibility ........................................................ pp. 8-9
C18 Selectivity .............................................................................. p. 10
Utilizing Hydrophobicity .............................................................. p. 11
Polar C18 Selectivity .................................................................... p. 12
Enhanced Polar Retention ......................................................... p. 13
100% Aqueous Stability .............................................................. p. 14
Polar and Non-polar Selectivity ................................................. p. 15
Upgrading C18 Methods ............................................................. p. 16
Improving Existing Polar Methods ............................................. p. 17
Polar Case Study: Catecholamines and PMETs ....................... p. 18
Polar Case Study: ETG/ETS ........................................................ p. 19
C18 and Polar C18 - Complementary Work Horses ........ pp. 20-21
Pairing With Core-Shell Technology .................................. pp. 22-23
Column Lifetime ........................................................................... p. 24
Sample Preparation ..................................................................... p. 25
Column Protection – Guard Cartridge System ........................ p. 26
Ordering Information ................................................................... p. 27
Why Should Luna® Omega be in your lab
Om
eg
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18P
ola
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18R
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© 2016 Phenomenex, Inc. All rights reserved.
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4
Within the novel manufacturing process of Luna Omega 1.6 µm silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity.
Most importantly, through our proprietary process, we eliminate micropores, further improving column efficiency, inertness, and reproducibility.
Consistent Porosity
Absence of Micropores
Luna® is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, repro-ducibility and dependability for a wide range of analyses. The new Luna Omega 1.6 μm builds upon this legacy with an innovative yet rugged UHPLC silica particle architecture, designed and manufactured by Phenomenex based on more than 20 years of applied knowledge, invention, and customer experience.
Novel Design and Manufacturing Process
Cutting-Edge 1.6 μm Silica Particle
TMSTMS
PolarPolar
TMSTMS
Thermal Modified Pore
Structure
Luna Omega C18
Luna Omega Polar C18
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5
Consistent Porosity
Absence of Micropores
The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hy-brid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.
UHPLC Efficiency Comparison
Comparative separations may not be representative of all applications.
Efficiency (plates/meter)
Luna® Omega 1.6 µm C18
Thermo Scientific® Hypersil GOLD® 1.9 µm C18
Agilent® ZORBAX® Eclipse 1.8 µm XDB-C18
Thermo Scientific Syncronis™ 1.7 µm C18
Waters® ACQUITY® HSS 1.8 µm C18
Waters ACQUITY BEH 1.7 µm C18
AkzoNobel® Kromasil® 1.8 µm C18
ACE® Excel™ 2 µm C18
YMC®-Triart 1.9 µm C18
Agilent ZORBAX 1.8 µm SB-C18
0 50,000 100,000 150,000 200,000 250,000 300,000 350,000
Conditions for all columns:Dimension: 50 x 2.1 mm
Mobile Phase: Acetonitrile/Water (65:35)Flow Rate: 0.5 mL/min
Temperature: AmbientDetection: UV @ 254 nm
System: ACQUITY UPLC®
Astounding PerformanceCutting-Edge 1.6 μm Silica Particle
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6
01.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6
1 2 3 4 5 6 min
23441
1 2 3 4 5 6 min0.0
1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6
5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e57.0e57.5e5
1 2 3 4 5 6 min0.0
23440
Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.
Comparative separations may not be representative of all applications.
Conditions for all columns:Columns: Luna Omega 1.6 µm C18
ZORBAX 1.8 µm XDB-C18ACE Excel 2 µm C18-AR
Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in MethanolGradient: Time (min)
07
% B390
Flow Rate: 0.3 mL/minTemperature: 30 °C
Detection: MS/MSSample: 1. Succinic acid
2. MMA3. Glutaric acid4. Methylsuccinate5. Ethylmalonic acid6. Hippuric acid7. Homovanillic acid8. Suberic acid
Luna® Omega 1.6 µm C18
ACE® Excel™ 2 µm C18-AR
Agilent® ZORBAX® 1.8 µm XDB-C18
Ap
p ID
234
39A
pp
ID 2
3441
Ap
p ID
234
40
Inte
nsity
, cp
sIn
tens
ity, c
ps
Inte
nsity
, cp
s
Separation Muscle
Much Greater Resolution!
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7
0.05
0.1
0.15
0.2
0.25AU
0
min0 1 2 3 4
1
4
5,2
6
Inert Foundation
Luna Omega UHPLC columns contain a unique silica modified using a propri-etary, post-synthetic thermal treatment process to provide extraordinary me-chanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that nega-tively affect peak shape, allowing for greater method accuracy.
Waters® ACQUITY® BEH 1.7 µm C18
Thermo Scientific® Hypersil GOLD® 1.9 µm C18
Comparative separations may not be representative of all applications.
Conditions for all columns:Columns: Luna Omega 1.6 µm C18
ACQUITY BEH 1.7 µm C18Hypersil GOLD 1.9 µm C18
Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)
056
6.18
% B5959555
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: 1. Pindolol
2. Chlormipramine3. Nortripytline 4. 3-Methyl-4-nitrobenzoic acid5. 5-Methylsalicylate6. Hexanophenone
Luna® Omega 1.6 µm C18
Poor Peak Shape
Separation Muscle
0.025
0.05
0.075
0.1
0.125
0.15
0.175
AU
0
min0 1
1
2
3
4
5
6
2 3 4
Excellent Peak Shape
Ap
p ID
234
29A
pp
ID 2
3431
0.05
0.1
0.15
0.2
min0 1 2 3 4
AU
0
1
2
3
4
5
6
Ap
p ID
234
30
Tailing Has Caused Poor Quantitation
Peak #3 is Adsorbed
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8
Consistent Results – C18
Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.
Luna Omega C18 – Batch A
0
0.2
0.4
0.6
0.8
1
1.2
1.4
10 2 3 4 5 min
1
23 4
56
7
8
Ap
p ID
235
23
Luna Omega C18 – Batch B
0
0.2
0.4
0.6
0.8
1
1.2
1.4
10 2 3 4 5 min
1
23 4
56
7
8A
pp
ID 2
3523
Luna Omega C18 – Batch C
0
0.2
0.4
0.6
0.8
1
1.2
1.4
10 2 3 4 5 min
1
23 4
56
7
8
Ap
p ID
235
23
Conditions for all columns:Columns: Luna Omega 1.6 µm C18
Dimension: 50 x 2.1 mmPart No.: 00B-4742-AN
Mobile Phase: A: WaterB: Acetonitrile
Gradient: Time (min)06
6.018
% B20602020
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 220 nmSample: 1. Estriol
2. Prednisolone3. Hydrocortisone4. Cortisone5. Cortisone Acetate6. 21-Hydroxycortisone7. 17-Hydroxycortisone8. Deoxycorticosterone
Peak Capacity
270
Peak Capacity
263
Peak Capacity
270
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9
Consistent Results – Polar C18
Just like Luna® Omega C18, the Luna Omega Polar C18 is also stringently test-ed to make sure both batch-to-batch and column-to-column quality stays at the highest level before it reaches your lab bench.
Luna Omega Polar C18 – Batch A
25
0
50
75
100
120
100
150
175
10 2 3 4 min
12
34
5 6
7
Ap
p ID
235
70
Luna Omega Polar C18 – Batch B
25
0
50
75
100
120
100
150
175
10 2 3 4 min
1 2
34
5 6
7A
pp
ID 2
3570
Luna Omega Polar C18 – Batch C
25
0
50
75
100
120
100
150
175
10 2 3 4 min
1 2
34
5 6
7
Ap
p ID
235
70
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Dimension: 50 x 2.1 mmPart No.: 00B-4748-AN
Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile
Gradient: Time (min)0 5
% B595
Flow Rate: 0.4 mL/minTemperature: 30 °C
Detection: UV @ 254 nmSample: 1. Uracil
2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methylsalicylate7. Hexanophenone
Peak Capacity
163
Peak Capacity
166
Peak Capacity
162
QUALITYMANAGEMENT SYSTEMCERTIFIED BY DNV GL
9001:2008
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10
Selectivity Highlight Luna Omega C18
Luna® Omega C18 is an excellent first choice for chromatographers who are just starting method de-velopment or attempting to improve upon existing chromatographic results with other C18s. With its higher performance potential, excellent retention profile, and greater inertness, the Luna Omega C18 was designed to be the new all-purpose UHPLC solution for industries all over the world.
Phase C18
Particle Size 1.6 µm
Pore Size 100 Å
pH Range 1.5 - 8.5*
Surface Area 260 m2/g
Carbon Load 11 %
Pressure Limit 1000 bar
USP Listing L1
Greater Retention and Better ResultsHigher efficiency levels in combination with excellent stationary phase coverage and greater particle inert-ness, translates to improved separation power for you. Now you can utilize the greater retention of Luna Omega C18 to tackle both easy and difficult separations.
Luna Omega 1.6 µm C18
Waters® ACQUITY® BEH 1.7 µm C18
min0 0.5 1 1.5 2 2.5
AU
0
11
2 3
4
5 6
7
9
8
min0 0.5 1 1.5 2 2.5
AU
0
1
12
3
4
5 6
7
9
8
Ap
p ID
234
85
Ap
p ID
234
82
*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
TMSTMS
Conditions for all columns:Columns: Luna Omega 1.6 μm C18
ACQUITY BEH 1.7 μm C18Dimension: 50 x 2.1 mm
Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile
Gradient: Time (min)0 3
3.5 3.51
5
% B10 55 55 10 10
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 205 nmSample: 1. Acetaminophen
2. 4-Aminobenzoic Acid3. 4-Hydroxybenzoic Acid4. 2-Acetaminophenol5. 3-Hydroxybenzoic Acid6. Salicylicamide7. Phenol8. Benzoic Acid9. Salicylic Acid
Peak Capacity
196
Peak Capacity
160
Greater Peak Capacity and Retention
Comparative separations may not be representative of all applications.
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11
Small and Large Compound Mixtures
Strong and focused hydrophobic retention, incredible efficiency and valuable in-ertness of Luna® Omega C18 columns make them an excellent choice for small mixtures of compounds differing in hydrophobicity as well as large mixtures of compounds like impurity/degradation profiles and peptide maps.
Phenols
OTC Drug - Pill Formulation Profile
Synthetic Cannabinoids
Peptide Map – Digested BSA
AU
-0.005
0
0.005
0.01
0.015
0.02
0.025
min0 1
1
2
2
3
3
45
67
4 50.0
1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e6
3 4 5 6 7 8 9 min
Ap
p ID
234
42
Columns: Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm
Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)
067
7.19
% B5505055
Flow Rate: 0.4 mL/minTemperature: 22 °C
Detection: UV @ 270 nmSample: 1. 3-Hydroxyphenol
2. Phenol3. 4-Nitrophenol4. 4-Methylphenol5. 2-Methylphenol6. 2,4-Dimethylphenol7. 1-Naphthol
Columns: Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm
Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)
06
% B50100
Flow Rate: 0.3 mL/minTemperature: 30 °C
Detection: MS/MS (SCIEX API 4000™)Sample: 1. JWH-073 Butanoic acid metabolite
2. JWH-073 3-hydroxybutyl metabolite 3. JWH-018 Pentanoic acid metabolite 4. JWH-073 4-hydroxybutyl metabolite 5. JWH-018 4-hydroxypentyl metabolite 6. AM-2201 4-hydroxypentyl metabolite 7. JWH-018 5-hydroxypentyl metabolite 8. AM-694 9. AM-220110. JWH-073 11. JWH-018
Ap
p ID
234
34
0.01
0.02
0.03
0.04
0
AU
0 1 2 3 4 min0.00
1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9
10 20 30 min
Ap
p ID
236
00
Columns: Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm
Part No.: 00B-4742-ANMobile Phase: A: 20 mM Potassium Phosphate pH 7.2
B: AcetonitrileGradient: Time (min)
056
6.18
% B5707055
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: OTC Drug Pill
Columns: Luna Omega 1.6 µm C18Dimension: 100 x 2.1 mm
Part No.: 00D-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)
050
50.1
% B3503
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000)Sample: Tryptic digest of BSA
Ap
p ID
235
17
Inte
nsity
, cp
s
Comparative separations may not be representative of all applications.
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12
Selectivity Highlight Luna Omega Polar C18
Luna® Omega Polar C18 is a novel UHPLC station-ary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-pur-pose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides en-hanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.
Phase Polar C18
Particle Size 1.6 µm
Pore Size 100 Å
pH Range 1.5 - 8.5*
Surface Area 260 m2/g
Carbon Load 9 %
Pressure Limit 1000 bar
USP Listing L1
A C18, But DifferentLuna Omega Polar C18 is a uniquely modified C18-based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize UHPLC for mixtures of polar and non-polar compounds.
Luna Omega C18 silica surface
TMSTMS
Luna Omega Polar C18 silica surface
TMS
TMS
*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
PolarPolar
TMSTMS
PolarPolar
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13
Greater Polar Retention
With its polar modified surface, the Luna® Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.
Nicotine and Metabolites
Catecholamines
02.0e5
2 min1
4.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e6
1+2 3
4
5
1.0e5
2.0e5
3.0e64.0e6
5.0e6
6.0e67.0e6
8.0e6
02 min
2
3 4
5
1
1
2.0e54.0e56.0e58.0e61.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e6
02 min1
1+23
4
5
1.0e5
2.0e5
3.0e6
4.0e6
5.0e6
6.0e6
7.0e6
8.0e6
02 min
2
34
5
1
1
Ap
p ID
235
95A
pp
ID 2
3596
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
ACQUITY BEH 1.7 µm C18Dimension: 100 x 2.1 mm
Mobile Phase: A: Water with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid
Gradient: Time (min)03
3.1
% B0900
Flow Rate: 0.4 mL/minTemperature: 50 °C
Detection: MS/MS (SCIEX API 4000)Sample: 1. Norepinephrine
2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine
Ap
p ID
235
94A
pp
ID 2
3591
Inte
nsity
, cp
sIn
tens
ity, c
ps
Inte
nsity
, cp
sIn
tens
ity, c
ps
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
CORTECS 1.6 µm C18Dimension: 50 x 2.1 mm
Mobile Phase: A: 10 mM Ammonium Formate with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid
Gradient: Time (min)03
3.1
% B2902
Flow Rate: 0.4 mL/minTemperature: 25 °C
Detection: MS/MS (SCIEX API 4000™)Sample: 1. Nornicotine
2. 3-Hydroxycotinine3. Nicotine4. Cotinine5. Anabasine
Greater Retention
Greater Retention
Luna Omega 1.6 μm Polar C18
Waters® CORTECS®
1.6 µm C18
Waters® ACQUITY® BEH 1.7 µm C18
Luna Omega 1.6 µm Polar C18
Comparative separations may not be representative of all applications.
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14
100 % Aqueous Stability
No Stationary Phase CollapseTraditional C18 phases are known to collapse under 100% aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna® Omega Polar C18 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C18 in 100% aqueous buffer conditions for over 2 weeks.
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Dimension: 50 x 2.1 mmPart No.: 00B-4748-AN
Mobile Phase: 10 mM Ammonium Formate with 0.1 % Formic Acid
Flow Rate: 0.4 mL/min
Temperature: 22 °CDetection: UV @ 254 nm
Sample: 1. Adenine2. Guanosine-5-Monophosphate3. Thymine
Aqueous Stability of Luna Omega Polar C18
% o
f Ini
tial R
eten
tion
Tim
e
Adenine G-5-MP Thymine
100%110%
80%
60%
40%
20%
0%
Day 1
Day 25
Nucleosides in 100 % Aqueous Conditions
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
ACQUITY BEH 1.7 µm C18Dimension: 50 x 2.1 mm
Mobile Phase: 20 mM Ammonium Formate pH 3.0Flow Rate: 0.4 mL/min
Temperature: 22 °C
Detection: UV @ 285 nmSample: 1. Thiourea
2. 5-Fluorocytosine3. Guanosine-5-Monophosphate4. Adenine5. Thymine
0.080
0.0100.020
0.030
0.040
0.050
0.060
0.070
1
2
3 4 5
min0 1 2
0
0.080
0.0100.020
0.030
0.040
0.050
0.060
0.070
1
2
34 5
min0 1 2
0
23585
Ap
p ID
235
85
Ap
p ID
235
86
Ap
p ID
235
77
Use 100% aqueous conditions for the greatest polar acid/base retention.
Luna Omega 1.6 µm Polar C18
Waters® ACQUITY® BEH 1.7 µm C18
Comparative separations may not be representative of all applications.
Duration of Exposure
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15
100 % Aqueous Stability
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0 2 4 6 8 10 min
RT: 0.00 - 12.00
RT: 2.97AA: 96610SN: 1654
RT: 1.84AA: 7670SN: 36
RT: 1.93AA: 508SN: 4
RT: 2.68AA: 384SN: 10
RT: 8.23AA: 847097SN: 2491
RT: 9.77AA: 313SN: 10
RT: 7.62AA: 3628908SN: 4156
RT: 9.17AA: 167SN: 13
NL: 7.27E3TIC F: + c ESI SRM ms2 115.000 [98.099-98.101] MS ICIS Test03b
NL: 3.53E4TIC F: + c ESI SRM ms2 166.100 [149.099-149.101] MS ICIS Test03b
NL: 1.65E5TIC F: + c ESI SRM ms2 171.100 [125.099-125.101] MS ICIS Test03b
RT: 1.32AA: 373SN: 34
RT: 4.64AA: 179SN: 35
NL: 3.83E3TIC F: + c ESI SRM ms2 416.000 [194.099-194.101]MS ICIS Test03b
Versatility – Hydrophobic and Polar Interactions
Combined retention of polars and non-polars by the Luna® Omega Polar C18 gives you an incredible range of potential success when working with methods from just about any industry. Here we illustrate the useful-ness of these interaction mechanisms with water soluble vitamins from drink and tablet, a multi-compound antibiotic screen, and a UHPLC/MS/MS separation of algal toxins.
Antibiotic Screen
Find complete method at Phenomenex.comApplication ID 23569
1.0e6
2.0e6
3.0e6
4.0e6
min0 1 2 3 4 50
Ap
p ID
235
87
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Dimension: 50 x 2.1 mmPart No.: 00B-4748-AN
Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)04
4.17
% B09000
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000™)Sample: 1. Pyridoxamine
2. Thiamine3. Nicotinic acid4. Pyridoxine
5. Pantothenic acid6. Folic acid7. Riboflavin
Inte
nsity
, cp
s
2.0e5
1.0e5
3.0e5
5.0e56.0e5
4.0e5
2 3 4 min10
2 3
4
5
1.0e6
2.0e6
3.0e6
4.0e6
4.5e6
2 3 4 min10
2
4
5
6 71
Ap
p ID
235
78A
pp
ID 2
3579
Inte
nsity
, cp
s
Rel
ativ
e A
bun
dan
ce
Inte
nsity
, cp
s
02.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e6
2
2
3
3
4
4
56
7
min1
1
Ap
p ID
235
84
Standards
Uracil-d4
Vitamin Enriched Drink
Anatoxin-a
Vitamin Tablet
L-Phenylalanine-d5
Cylindrospermopsin
Columns: Luna Omega 1.6 µm Polar C18Dimension: 50 x 2.1 mm
Part No.: 00B-4748-ANMobile Phase: A: 10 mM Ammonium formate with
0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)0
0.55
5.1
% B09500
Flow Rate: 0.5 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000)
Water Soluble Vitamins Algal Toxins (EPA 545)
Comparative separations may not be representative of all applications.
1. Sulfaguanidine 2. Sulfadiazine 3. Cefalexin 4. Lincomycin 5. Amoxicillin 6. Sulfathiazole 7. Sulfapyridine 8. Cefazolin 9. Sulfamerazine 10. Ciprofloxacin 11. Sulfamethazine 12. Sulfamonomethoxine 13. Enrofloxacin 14. Difloxacin 15. Chlortetracycline 16. Sulfamethoxazole 17. Florfenicol 18. Sulfaquinoxaline 19. Oxacillin 20. Sulfadimethoxine
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16
Highly Useful Alternative to the Common C18
Unlike traditional UHPLC C18 stationary phases, the polar and hydrophobic ver-satility of Polar C18 combined with its high efficiency levels allow for enhanced separation power. Combine that with 100 % aqueous stability and you can really see how the Luna® Omega Polar C18 can potentially lead to more separation success compared to traditional C18 columns.
Ketotifen Impurity Profile
Beta Blockers
0.005
0.010
0.015
0.020
0.025
0.030
0
2 3 4 5 min1
0.005
0.010
0.015
0.020
0.025
0.030
0.010
0.015
0.020
0.025
0.030
0.035
02 3 4 5 min1
0.005
2
3
45
6
1
0.005
0.010
0.015
0.020
0.025
0.030
0
2 3 4 5 min1
0.005
0.010
0.015
0.020
0.025
0.030
0.010
0.015
0.020
0.025
0.030
0.035
0
0.005
2
2
3
3
4
4
5
56
min1
1
Ap
p ID
236
05A
pp
ID 2
3612
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Hypersil GOLD 1.9 µm C18Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)0
2.5610
10.0113
% B515203055
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: Ketotifen
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
ZORBAX 1.8 µm SB-C18Dimension: 100 x 2.1 mm
Mobile Phase: A: 20 mM Postassium Phosphate pH 7.2B: Acetonitrile
Gradient: Time (min)0
2.5
% B535
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: UV @ 280 nmSample: 1. Atenolol
2. Pindolol3. Timolol4. Metoprolol5. Labetolol6. Propranolol
Ap
p ID
236
04A
pp
ID 2
3611
Inte
nsity
, cp
sIn
tens
ity, c
ps
Inte
nsity
, cp
sIn
tens
ity, c
ps
Luna Omega 1.6 µm Polar C18
Luna Omega 1.6 µm Polar C18
Thermo Scientific® Hypersil GOLD® 1.9 µm C18
Agilent® ZORBAX® 1.8 µm SB-C18
Clean, sharp peaks and excellent resolution
Greater retention and resolution of impurities
Comparative separations may not be representative of all applications.
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17
Excellent Alternative to Existing Polar Columns
Luna® Omega Polar C18 is an incredibly high performing UHPLC column with a unique selectivity profile that can be used to upgrade existing methods or jumpstart new method development. Realize lower limits of detection with the increase in sensitivity levels or just utilize the efficiency gains to help resolve closely eluting peaks.
Mycotoxins Screen
SAMSHA Workplace Drug Testing Screen
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
1.0e5
0
1
3+4
256
78
1 2 3 4 5 6 min
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
1.0e5
01 2 3 4 5 6 min
23571
2
3+4
5
67 8
1
2.0e5
0
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e61.8e6
1 2 3 4 min
23589
2.0e5
0
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e61.8e6
23588
1 2 3 4 min
Ap
p ID
235
72
Ap
p ID
235
88
Ap
p ID
235
89
Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18
ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)023
3.1
% B1510010015
Flow Rate: 0.4 mL/minTemperature: 25 °C
Detection: MS/MS (SCIEX API 4000)Sample: 1. Morphine
2. Codeine 3. Amphetamine 4. MDA 5. Methamphetamine 6. 6-MAM 7. MDMA 8. MDEA 9. BZE 10. PCP 11. THC-COOH 12. THC
Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18
ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)04
4.16
% B20902020
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)
1. 15-Acetyldeoxynivalenol2. Aflatoxin G23. Aflatoxin G14. Aflatoxin B25. Aflatoxin B16. Aflatoxin M17. HT2 Toxin8. T2 Toxin
Ap
p ID
235
71
Inte
nsity
, cp
s
Inte
nsity
, cp
s
Inte
nsity
, cp
s
Luna Omega 1.6 µm Polar C18
Waters® ACQUITY® HSS 1.8 µm T3
Luna Omega 1.6 µm Polar C18
Waters® ACQUITY® HSS 1.8 µm T3
Comparative separations may not be representative of all applications.
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18
Polar Case Study – Catecholamines and Metabolites
Current analytical tests for pheochromocytoma (tumor of chromaffin cells of the adrenal medulla) focus on measuring for elevated levels of plasma free metanephrine and normetanephrine, which are continually se-creted by the tumors. Supplementary methods also analyze the metabolites of metanephrine and normeta-nephrine, including vanillylmandelic acid. With such a range of polar compounds, the aqueous stability and enhanced polar retention of the Luna® Omega Polar C18, both play an strong part in generating excellent separation between all these important analytes.
Catecholamines (ESI+)
PMETs from Plasma (ESI+)
Catecholamine Metabolites (ESI-)Flow Rate: 0.3 mL/min
Ap
p ID
235
91A
pp
ID 2
3592
Ap
p ID
235
93
Inte
nsity
, cp
sIn
tens
ity, c
ps
Inte
nsity
, cp
s
1.0e5
2.0e5
3.0e6
4.0e6
5.0e6
6.0e6
7.0e6
8.0e6
02
2
3
3 4
5
6
4 min1
1
1.0e5
2.0e5
3.0e6
4.0e6
5.0e6
6.0e6
7.0e6
8.0e6
02 3
34
5
6
9
4 min1
1.0e5
2.0e5
3.0e6
4.0e6
5.0e6
02
8
7
9
3 min1
Conditions for all columns unless otherwise noted:Columns: Luna Omega 1.6 µm Polar C18
Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN
Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic acid
Gradient: Time (min)03
3.1
% B0900
Flow Rate: 0.4 mL/minTemperature: 50 °C
Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)
1. Norepinephrine2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine6. 3-Methoxy tyramineNegative Mode (ESI-)7. Vanillylmandelic acid (VMA)8. 5-Hydroxyindoleacetic acid (5-HIAA)9. Homovanillic acid (HVA)
Recommended Sample Preparation MethodProduct: Strata-X-CW Microelution 96-Well PlatePart No.: 8M-S035-4GA
Condition: 200 µL MethanolEquilibrate: 200 µL Water
Load Sample: 250 µL Plasma diluted with 250 µL WaterWash 1: 200 µL WaterWash 2: 200 µL 50:50 Acetonitrile/IPA
Dry for 1 minute at 10” Hg to remove excess wash solvent
Elute: 2 x 25 µL 85:15 Acetonitrile/Water with 2 % Formic acid
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19
Polar Case Study – ETG/ETS
Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites of ethanol that are used by analytical laboratories to detect recent ethanol ingestion. In this application, the Luna® Omega Polar C18 provides excellent separation between EtG and EtS, along with valuable separation from a urine interference peak and where other matrix suppression is common. Additionally, the high efficiency lev-els provided by the Luna Omega 1.6 µm allow for a highly sensitive, yet rapid method.ETG and ETS from Urine Sample
Ap
p ID
235
97A
pp
ID 2
3597
Ap
p ID
235
97
Inte
nsity
, cp
sIn
tens
ity, c
ps
Inte
nsity
, cp
s
2000400060008000
0
1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4
min2 31
2
3
1
200100
0
400300
600500
800700
9001000
12001100
13001365
2 3 min1
3
1
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN
Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)01
1.15
% B05000
Flow Rate: 0.3 mL/minTemperature: 25 °C
Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)
1. Urine interference2. ETS3. ETG
Overlaid XIC
XIC to ETS
XIC to ETG
2000400060008000
0
1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4
min2 31
2
1
ETS
ETG
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20
Complementary UHPLC Work Horses
Combine the versatile interaction mechanisms of the Luna® Omega C18 and Polar C18 to achieve successful separations and improve upon challenging ex-isting methods.
Natural Cannabinoids
Influence Selectivity with 100 % Aqueous Stability and Polar Interactions
Intact Peptides
Ap
p ID
235
81
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Luna Omega 1.6 µm C18Dimension: 100 x 2.1 mm
Mobile Phase: A: 20 mM Ammonium Formate pH 3.2B: Acetonitrile
Gradient: Time (min)01213
13.0115
% B6095956060
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: UV @ 256 nmSample: 1. CBVD
2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A
Conditions for all columns except where noted:Columns: Luna Omega 1.6 µm Polar C18
Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % TFAB: Acetonitrile with 0.1 % TFA
Gradient: Time (min)015
% B0065
Flow Rate: 0.6 mL/minTemperature: 25 °C
Detection: UV @ 210 nmSample: 1. Gly-Tyr
2. Val-Tyr-Val3. Met-Enkephalin4. Leu-Enkephalin5. Angiotensin II
Ap
p ID
235
80
Inte
nsity
, cp
sIn
tens
ity, c
ps
0
2 3 4 5 min1
200
400
600
mAU
0
2 3 4 5 min1
200
400
600
mAU
Ap
p ID
236
06
Ap
p ID
236
09
0
4 6 7 8 9 105 min3
0.010
0.020
0.030
0.040
0.050
0.060
0.070mAU
0
4 6 7 8 9 105 min3
0.010
0.020
0.030
0.040
0.050
0.060
0.070mAU
Use the 100 % aqueous stability of polar C18 to aid in better retention of polar compounds
Luna Omega 1.6 µm Polar C18
Greater hydrophobic retention
Luna Omega 1.6 µm C18
Luna Omega 1.6 µm Polar C18
Luna Omega 1.6 µm C18
Comparative separations may not be representative of all applications.
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21
Complementary UHPLC Work Horses
For the analysis of a large screen or mixture of compounds, you can benefit greatly from utilizing both the Luna® Omega C18 and Polar C18 during method development. The strong focused hydrophobic retention of the C18 will provide excellent non-polar retention and separation, while the Polar C18 provides a complementary hydro-phobic/polar combined selectivity that can retain and separate typically early eluting compounds.
Tryptic Digest - Peptide Map of BSA
Conditions same for all columns:Columns: Luna Omega 1.6 µm C18
Luna Omega 1.6 µm Polar C18Dimension: 100 x 2.1 mm
Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile
Gradient: Time (min)050
50.1
% B3503
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000™)Sample: Digested BSA
Inte
nsity
, cp
s
1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e92.00e93.00e94.00e9
14 16 18 20 22 24 26 28 30 32 34 36 38 min2 4 6 8 10 12
Ap
p ID
235
99
Inte
nsity
, cp
s
0.001.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9
10 20 30 min
Ap
p ID
236
00
Inte
nsity
, cp
s
Inte
nsity
, cp
s
018 19 min17
2.00e8
4.00e8
6.00e8
8.00e8
10.00e8
12.00e8
Ap
p ID
236
01
Ap
p ID
236
01
Luna Omega 1.6 µm Polar C18
Luna Omega 1.6 µm
Polar C18
Luna Omega 1.6 µm C18
Luna Omega 1.6 µm C18
Greater resolution of early, more polar eluters
Better resolution of more hydrophic compounds
Comparative separations may not be representative of all applications.
018 19 min17
2.00e8
4.00e8
6.00e8
8.00e8
10.00e8
12.00e8
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22
Luna Omega + Kinetex = Happy UHPLC
Increase your method development options by screening a combination of core-shell and fully porous selectivities.
Natural Cannabinoids
Inte
nsity
, cp
sIn
tens
ity, c
ps
0
4 6 7
1 2 37 8
910
4
5
6
8 9 105 min3
0.010
0.020
0.030
0.040
0.050
0.060
0.070mAU
0.010
0.020
0.030
0.040
0.050
0.060
0.070
0
4 6 7 8 9 105 min3
mAU
178
9 10
2-4
5
6
Ap
p ID
235
82A
pp
ID 2
3580
Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18
Kinetex 1.7 µm BiphenylKinetex 1.7 µm F5
Dimension: 100 x 2.1 mmMobile Phase: A: 20 mM Ammonium Formate pH 3.2
B: AcetonitrileGradient: Time (min)
01213
13.0115
% B6095956060
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: UV @ 256 nmSample: 1. CBVD
2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A
Luna® Omega 1.6 µm Polar C18
Kinetex 1.7 µm Biphenyl
TMSTMS
PolarPolar
TMSTMS
Biphenyl
Inte
nsity
, cp
s
0
0.010
0.020
0.030
0.040
0.050
0.060
0.070
4 6 7 8 9 105 min3
mAU
1 2 3 7 8
910
4
5
6
Ap
p ID
235
83
Kinetex® 1.7 µm F5
F5
F
F
F
F
F
TMSTMS
Excellent resolution
Faster analysis with similar peak order
Alternative peak elution order
Comparative separations may not be representative of all applications.
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23
Conditions for all columns except where noted:Columns: Luna Omega 1.6 µm C18
Kinetex 1.7 µm BiphenylKinetex 1.7 µm EVO C18
Dimension: 50 x 2.1 mmMobile Phase: Luna Omega 1.6 µm C18 and Kinetex 1.7 µm Biphenyl
A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in AcetonitrileKinetex 1.7 µm EVO C18A: 10 mM Ammonium Bicarbonate pH 10B: Acetonitrile
Gradient: Time (min)045
5.1
% B595955
Flow Rate: 0.4 mL/minTemperature: 40 °C
Detection: MS/MS (SCIEX API 4000™)Sample: Drugs of Abuse
Luna Omega + Kinetex =Happy UHPLC
Combining orthogonal UHPLC particle morphologies and selectivities like the Luna Omega C18, Kinetex Biphenyl, and Kinetex EVO C18 will drastically in-crease your probability of separation success!
Morphine
Drugs of Abuse Analysis Luna® Omega 1.6 µm C18
Kinetex® 1.7 µm Biphenyl
Kinetex 1.7 µm EVO C18A
pp
ID 2
3444
1 2 3 4 min
23443
1 2 3 4 min
1 2 3 4 min
Ap
p ID
234
43
pH 2.5
pH 2.5
pH 8.5
ADVANTAGE Excellent inertness and
strong hydrophobic retention
ADVANTAGE Extra aromatic (pi-pi)
and dipole-dipole retention
ADVANTAGE Excellent peak shape and
retention of bases at high pH
Ap
p ID
234
45
Comparative separations may not be representative of all applications.
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24
Excellent Lifetime
Luna Omega 1.6 µm media was designed to be durable and withstand the high pressures and rigors of UHPLC work in combination with providing excellent performance.
Accelerated Lifetime Study
% In
crea
se o
ver
Initi
al
Bac
kpre
ssur
e
Injection Cycle
Luna® Omega 1.6 µm C18 (AVG)
Waters® ACQUITY BEH 1.7 µm C18 (AVG)
0 10 20 30 40 50 600.0%
1.0%
2.0%
3.0%
4.0%
5.0%
6.0%
7.0%
8.0%
9.0%
10.0%
Ap
p ID
236
10
Conditions same for both columns:Columns: Luna Omega 1.6 µm C18
ACQUITY BEH 1.7 µm C18Dimension: 50 x 2.1 mm
Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile
Gradient: Time (min)04
4.1
% B5955
Flow Rate: 0.4 mL/minTemperature: 25 °C
Detection: UV @ 210 nmSample: Protein Matrix
Comparative separations may not be representative of all applications.
Extend UHPLC Column Lifetime Even More with SecurityGuard ULTRASee page 26 for details
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25
Further Improve Lifetime with Sample Preparation Both UHPLC instrumentation and columns can benefit from consistent removal of microparticulates/contaminants that may appear in solvents, samples or spe-cific matrices. Over time these problematic compounds can build up on instru-mentation and columns, leading to the need for costly maintenance and repair. Strata-X polymeric SPE, Novum liquid extraction, and Phenex syringe filters are excellent options to try.
Convenient and inexpensive way to remove particulates from LC samples
www.phenomenex.com/Phenex
novumsimplified liquid extraction
Polymeric SPE
Very targeted form of sample preparation that allows you to isolate your analyte of interest while removing any interfering compounds in your sample
www.phenomenex.com/strataX
Remove interferences such as proteins, phospholipids, salts, and more in a quick and simple procedure
www.phenomenex.com/Novum
Sample Preparation
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26
Protect any UHPLC Column
Protect your UHPLC column, including Luna® Omega columns, from dam-aging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system!
• Simple to use
• Extend column lifetime
• Pressure rated to 20,000 psi (1,378 bar)
• Fits virtually all manufacturers’ columns 2.1 to 4.6 mm ID
UHPLC Column Protection
See it in action: www.phenomenex.com/SecurityGuardULTRA
Secu
rityGu
ard™ U
LTR
AU
HP
LC C
olumn to D
etectorFrom
Injector
CartridgeHolder
SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder)
* Cartridge schematic not drawn to scale
Compounds of Interest
Contaminants and Particulates
Material Chamber
FritContaminants Trapped
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27
Ordering Information
See it in action: www.phenomenex.com/SecurityGuardULTRA
Buy Luna® Omega Now
Ordering Information
Luna for HPLCDon’t miss out on classically wonderful Luna HPLC columns in a variety of selectivities and particle sizes:
2.5 µm, 3 µm, 5 µm, 10 µm C18(2), Si(2), C8(2), C5, Phenyl-Hexyl, PFP(2), HILIC, CN, NH2, SCX
Find them at: www.phenomenex.com/Luna
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If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within 45 days FOR A FULL REFUND.
1.6 μm Minibore Columns (mm)SecurityGuard™
ULTRA Cartridges‡
Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 3/pk
Polar C18 00A-4748-AN 00B-4748-AN 00D-4748-AN 00F-4748-AN AJ0-9505C18 00A-4742-AN 00B-4742-AN 00D-4742-AN 00F-4742-AN AJ0-9502
for 2.1 mm ID‡ SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000
28
www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]
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Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.Trademarks Luna, Kinetex, and Strata are registered trademarks and SecurityGuard, Novum, and Phenex are trademarks of Phenomenex. ACE is a registered trademark and Excel is a trademark of Advanced Chromatography Technologies Limited. Waters, ACQUITY, UPLC, and CORTECS are registered trademarks of Waters Corporation. Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. Thermo Scientific is a registered trademark and Syncronis is a trademark of Thermo Fisher Scientific. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone LLC. AkzoNobel and Kromasil are registered trademarks of AkzoNobel Pulp and Performance Chemicals AB. YMC is a registered trademark of YMC Co., Ltd. API 4000 is a trademark of AB SCIEX Pte. Ltd. AB SCIEX™ is being used under license.Disclaimer Phenomenex is not affiliated with Advanced Chromatography Technologies Limited, Agilent Technologies, AkzoNobel, Thermo Fisher Scientific, YMC, or Waters Corporation.Comparative separations may not be representative of all applications.© 2016 Phenomenex, Inc. All rights reserved.B
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