Wound Healing Activity of Jasminum grandiflorum Linn.
M Syam Mohan*, J A J Sunilson, Y Hussein, J Thomas, T R Sekharan
*Presentation By:Y HusseinMasterskill College of Nursing and HealthMalaysia
Introduction Wound may be defined as loss or breaking of cellular and
anatomic or functional continuity of living tissue.
In the traditional system of medicine, the leaves of Jasminum
grandiflorum, which belongs to the family oleaceae are used
in the form of decoction against dermatitis and inflammation
in gums.
Objective
In the view of these, I have designed this present work to
study the possible effects of different extracts of the leaves of
Jasminum grandiflorum on wound healing, also knows as
Jasmin plant in Malaysia
A literature survey revealed that the plant leaves has not been
scientifically investigated for its wound healing activity.
The plant Jasminum grandiflorum
Materials
Collection:
• Trivandrum, Kerala, India in the
year 2003.
Study conducted at:
• S B. College of Pharmacy, Tamil
Nadu, India and repeated by a
student in Masterskill College
Method of study
Air dried leaves around 500 gm were reduced to coarse powder.
The leaves extracts were prepared in
Pet ether
Chloroform
Ethanol
Water
Apparatus used : Soxhlet apparatus
Base used : Simple oitment B.P
Animal Model : Excision model externally.
Qualitative Chemical Examination
Sl no
Plant constituents Extracts
Pet ether Chloroform Ethanol Aqueous
1 Alkaloids - + + +
2 Glycosides - - - +
3 Carbohydrates - - + +
4 Steroids - - + -
5 Tannins - - + -
6 Saponins - - + +
7 Flavanoids - - + +
8 Gums and mucilage - - + +
9 Fixed oil & Fat + - - -
10 Proteins - - - -
11 Volatile oils - - - -
‘+’ Present ‘-’ Absent
Thin layer chromatography – profile
The extracts were subjected to TLC.
Stationary phase: Silica gel-G
Saturation Time : 0.5 hr
Mobile phase : Benzene : Chloroform : Methanol
3 : 1 : 1
Spraying reagent : Dragendroff’s regent
Pharmacological screening
Animal used : Wistar Albino Rats
Groups : Total Six groups
Animal per group: Six
Excision wound model
Animals After infliction of wound
Effects of various extract Effect of PE ext of J. grandiflorum
0
100
200
300
400
500
600
0 2 4 6 8 10 12 14 16 18
Day
Dia
me
ter
of
wo
un
d
(mm
2)
Control Standard PE ext
Effect of CF ext of J. grandiflorum
0
100
200
300
400
500
600
0 2 4 6 8 10 12 14 16 18
Day
dia
met
er o
f w
ou
nd
(m
m2)
Control Standard CF ext
Effect of EtOH of J. grandiflorum
0
100
200
300
400
500
600
0 2 4 6 8 10 12 14 16 18
Day
dia
met
er o
f w
ou
nd
(m
m2)
Control Standard EtOH ext
Effect of aq. ext of J. grandiflorum
0
100
200
300
400
500
600
0 2 4 6 8 10 12 14 16 18
Day
dia
me
ter
of
wo
un
d
(mm
2)
Control Standard Aq ext
Animal after healing of wound
Discussion and conclusion
The results revealed that all extract showed a significant
wound contracting ability.
The pharmacological study substantiate the use of plant extract
as a wound healing agent.
This plant shows the activity owing to the presence of
alkaloids, steroids and tannins.
So necessary steps to be taken in future to identify the active
principles, study the toxicity and other pharmacological
property
Acknowledgement
S. B College of Pharmacy , India.
Masterskill college of nursing and health,
Malaysia.
