Date post: | 08-Apr-2018 |
Category: |
Documents |
Upload: | sanka-de-silva |
View: | 223 times |
Download: | 0 times |
of 39
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
1/39
Delayed Fluorescence andLuminescence: A Versatile Tool
for Risk Assessment andMonitoring in Sustainable
DevelopmentAlexis Zrimec 1, Luka Drinovec 1, and
Maja Berden Zrimec 2
International Brokerage Event and Scientific Conference
"Sustainable Land Management and Vulnerable Ecosystems"15-17 January 2003, Ljubljana, SLOVENIA
1 Institute of Physical Biology, Slovenia2 Institute Bion, Slovenia
Keywords: light emission, stress detection, ecotoxicology, genotoxicity, nondestructive bioassays
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
2/39
The Problem
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
3/39
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
4/39
it is necessary to develop nondestructive,objective, and quantitative, stress and quality
indicators.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
5/39
The methods used range from purely physico-chemical measurements to biological assays and
ecological surveys
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
6/39
The methods used range from purely physico-chemical measurements to biological assays and
ecological surveys
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
7/39
The methods used range from purely physico-chemical measurements to biological assays and
ecological surveys
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
8/39
The methods used range from purely physico-chemical measurements to biological assays and
ecological surveys
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
9/39
There is a need to develop methods which would
share the sensitivity and quantitative nature of physico-chemical methods with the relevance of
biological methods and at the same time beversatile enough to be used in many areas of
environmental research
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
10/39
There is a need to develop methods which would
share the sensitivity and quantitative nature of physico-chemical methods with the relevance of
biological methods and at the same time beversatile enough to be used in many areas of
environmental research
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
11/39
There is a need to develop methods which would
share the sensitivity and quantitative nature of physico-chemical methods with the relevance of
biological methods and at the same time beversatile enough to be used in many areas of
environmental research
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
12/39
Possible Solution
Delayed Fluorescence
and Luminescence
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
13/39
Delayed fluorescence is the long-lived lightemission from previously illuminated biological
material, whereas luminescence is spontaneous,nonstimulated, ultra-weak light emission from living
cells (several orders of magnitude weaker thandelayed fluorescence).
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
14/39
Delayed fluorescence is the long-lived lightemission from previously illuminated biological
material, whereas luminescence is spontaneous,nonstimulated, ultra-weak light emission from living
cells (several orders of magnitude weaker thandelayed fluorescence).
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
15/39
Delayed fluorescence of chlorophyll is correlatedwith chlorophyll content, photosynthetic activity,plant stress, crop quality, and taxonomic group
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
16/39
Delayed fluorescence of chlorophyll is correlatedwith chlorophyll content, photosynthetic activity,plant stress, crop quality, and taxonomic group
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
17/39
Delayed fluorescence of chlorophyll is correlatedwith chlorophyll content, photosynthetic activity,plant stress, crop quality, and taxonomic group
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
18/39
Delayed fluorescence of chlorophyll is correlatedwith chlorophyll content, photosynthetic activity,
plant stress, crop quality, and taxonomic group
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
19/39
Delayed fluorescence can be used as anecotoxicological stress indicator, a physiologicalindicator of plant viability, a monitoring tool for
phytoplankton succession, density, composition andactivity
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
20/39
Delayed fluorescence can be used as anecotoxicological stress indicator, a physiologicalindicator of plant viability, a monitoring tool for
phytoplankton succession, density, composition andactivity
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
21/39
Delayed fluorescence can be used as anecotoxicological stress indicator, a physiologicalindicator of plant viability, a monitoring tool for
phytoplankton succession, density, composition andactivity
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
22/39
Delayed fluorescence of DNA can be used as agenotoxicity test, because the fluorescence signalcorrelates with stress to nuclear DNA - which can
be induced by genotoxic substances
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
23/39
Luminescence measurements from living cells arecorrelated with the elevated reactive oxygen
species concentrations and the cell cycle.Its uses can be in nondestructive detection of plantviral infections (or other stresses), growth rates and
tumors.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
24/39
The advantage of delayed fluorescence as well asluminescence measurements is the possibility of field measurements of intact organisms in situ as
well as laboratory assays and studies.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
25/39
The Innovation
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
26/39
There are numerous publications reporting the useof delayed fluorescence or luminescence for
addressing different issues in physiology, ecology,agronomy and medicine. However, the
methodology is still not standardised, nor thereexists a versatile device for routine measurements.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
27/39
There are numerous publications reporting the useof delayed fluorescence or luminescence for
addressing different issues in physiology, ecology,agronomy and medicine. However, the
methodology is still not standardised, nor thereexists a versatile device for routine measurements.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
28/39
Our proposal is to review all the uses of delayed
fluorescence and luminescence in order to design aversatile tool for risk assessment and monitoring
and to design and construct the necessary
measuring equipment. The device based on light-emission detection from cells, tissues and
organisms in the field or in the laboratory could thenbe used for a wide spectrum of environmental
studies.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
29/39
Our proposal is to review all the uses of delayed
fluorescence and luminescence in order to design aversatile tool for risk assessment and monitoring
and to design and construct the necessary
measuring equipment. The device based on light-emission detection from cells, tissues and
organisms in the field or in the laboratory could thenbe used for a wide spectrum of environmental
studies.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
30/39
Our proposal is to review all the uses of delayed
fluorescence and luminescence in order to design aversatile tool for risk assessment and monitoring
and to design and construct the necessary
measuring equipment. The device based on light-emission detection from cells, tissues and
organisms in the field or in the laboratory could thenbe used for a wide spectrum of environmental
studies.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
31/39
Our Contribution
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
32/39
Our main scientific interest is in studying light
phenomena in living matter. For that purpose wedesigned and constructed a system for measuringweak photon emission from cells, tissues or whole
organisms.
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
33/39
Our main scientific interest is in studying light
phenomena in living matter. For that purpose wedesigned and constructed a system for measuringweak photon emission from cells, tissues or whole
organisms.
motor
high voltage
photon countingunit
PMT
cooling systemshutter
sample
thermostat
light
lightguide
shutterp
filter weel
personalcomputer
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
34/39
We apply the system for measuring delayed
fluorescence of algae and higher plants in order todetect stress and measure some parameters of photosynthesis.
W h d ig d id bi t t f
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
35/39
We have designed a rapid biotest for ecotoxicological screening of environmental
contamination using the delayed fluorescence of Lemna minor and (a marine alga) Skeletonemacostatum . The methodology will also be used in
assessing the quality of soil, water, vegetation andcrops for ecofarming or precision farming.
We have designed a rapid biotest for
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
36/39
We have designed a rapid biotest for ecotoxicological screening of environmental
contamination using the delayed fluorescence of Lemna minor and (a marine alga) Skeletonemacostatum . The methodology will also be used in
assessing the quality of soil, water, vegetation andcrops for ecofarming or precision farming.
W f d l d d fl
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
37/39
We perform delayed and prompt fluorescencemeasurements of marine algae to set up a systemfor monitoring the toxic algal populations and algal
blooms
Expected Contribution of the
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
38/39
Expected Contribution of the
Partners joint research and development of the
methodologies in the different fields of useand/or development of the necessaryinstrumentation
manufacturing and/or marketing of theproposed new technology
8/6/2019 Zrimec Delayed Fluorescence and Luminescence
39/39
Thank You