THE DEVELOPMENT OF A XENOGRAFT-BASED SCAFFOLD FOR TENDON AND LIGAMENT RECONSTRUCTION BY THORSTEN MARKUS SEYLER A Dissertation Submitted to the Graduate Faculty of WAKE FOREST UNIVERSITY GRADUATE SCHOOL OF ARTS AND SCIENCES in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY MOLECULAR MEDICINE AND TRANSLATIONAL SCIENCE May 2014 Winston-Salem, North Carolina Approved By George J. Christ, Ph.D. (Committee Chair) Thomas L. Smith, Ph.D. (Doctoral Thesis Advisor) Gary G. Poehling, M.D. Mark E. Van Dyle, Ph.D. Michael F. Callahan, Ph.D.
Transcript
THE DEVELOPMENT OF A XENOGRAFT-BASED SCAFFOLD FOR TENDON AND LIGAMENT RECONSTRUCTION
BY
THORSTEN MARKUS SEYLER
A Dissertation Submitted to the Graduate Faculty of
WAKE FOREST UNIVERSITY GRADUATE SCHOOL OF ARTS AND SCIENCES
in Partial Fulfillment of the Requirements
for the Degree of
DOCTOR OF PHILOSOPHY
MOLECULAR MEDICINE AND TRANSLATIONAL SCIENCE
May 2014
Winston-Salem, North Carolina
Approved By
George J. Christ, Ph.D. (Committee Chair)
Thomas L. Smith, Ph.D. (Doctoral Thesis Advisor)
Gary G. Poehling, M.D.
Mark E. Van Dyle, Ph.D.
Michael F. Callahan, Ph.D.
ii
DEDICATION
There are a number of people without whom this thesis might not have been written, and
to whom I am greatly indebted. I dedicate this thesis to my parents Evelyn and Heinz
Werner. I hope that this achievement will complete their dream when they chose to give
me the best education they could and their unequivocal support throughout the years. I also
dedicate this thesis to my loving and supportive wife Tasha, who provided endless words
of encouragement and supported me each step of the way. She was always there cheering
me up and stood by me through the good times and bad times. Thank you for providing me
strength, courage and support that urge me to strive to achieve my goals in life.
iii
ACKNOWLEDGEMENTS
Foremost, I would like to express my sincere gratitude to my advisors Thomas L. Smith,
Ph.D., Mark E. Van Dyke, Ph.D., Gary G. Poehling, M.D. for their continuous support of
my research, for their patience, motivation, enthusiasm, and immense knowledge. Their
guidance helped me in all the time of research and writing of this thesis. I could not have
imagined better advisors and mentors for my Ph.D. study.
Besides my advisors, I would like to thank Patrick W. Whitlock, M.D. Ph.D. for being a
friend and incredible resource on both an academic and a personal level, for which I am
extremely grateful. This thesis would not have been possible without his help, support, and
insightful comments. I would like to acknowledge the rest of my thesis committee: Gary
G. Poehling, M.D., Michael F. Callahan, Ph.D., and George J. Christ, Ph.D. for their
encouragement, insightful comments, and hard questions.
My sincere thanks also goes to Beth P. Smith, Ph.D., for her personal support and great
patience during my time in the orthopaedic research laboratories.
Last but not the least, I would like to thank my fellow residents and lab mates in the
Orthopaedic Surgery Department at Wake Forest: Sandeep Mannava, M.D., Ph.D.;
Johannes F. Plate, M.D. PhD.; Daniel N. Bracey, M.D.; and Peter J. Apel, M.D., Ph.D. for
their support and friendship, the stimulating discussions, the sleepless nights we were
working together, and for all the fun we have had in the last few years. Thank you!
Acknowledgements...................................................................................................................iiiTable of Contents.................................................................................................................................ivList of Figures and Tables.................................................................................................................viList of Abbreviations.........................................................................................................................viiThesis Abstract......................................................................................................................................x
Rationale for Decellularization in Tendon and Ligament Tissue Engineering............................2Description of Decellularization Modalities...........................................................................................7References.......................................................................................................................................................11Figures and Tables........................................................................................................................................18
Chapter 2....................................................................................................................................19A Tissue-engineered Approach to Tendon and Ligament Reconstruction: Current Trends...................................................................................................................................................19
Abstract............................................................................................................................................................21Introduction.....................................................................................................................................................22Clinical Significance of Tissue Engineered Solutions......................................................................24Natural Alternative Graft Choices for Tendon and Ligament Reconstruction: Xenografts..29Summary and Conclusions.........................................................................................................................35References.......................................................................................................................................................37Figures and Tables........................................................................................................................................48
Chapter 3....................................................................................................................................51A Novel Process for Optimizing Musculoskeletal Allograft Tissue: Improving Safety, Ultra-Structural Properties and Cell Infiltration......................................................................51
Abstract............................................................................................................................................................53Introduction.....................................................................................................................................................55Materials & Methods...................................................................................................................................57Results..............................................................................................................................................................64Discussion.......................................................................................................................................................68References.......................................................................................................................................................72Figures and Tables........................................................................................................................................78
Chapter 4....................................................................................................................................87The Development of a Xenograft-derived Scaffold for Tendon and Ligament Reconstruction....................................................................................................................................87
Abstract............................................................................................................................................................89Introduction.....................................................................................................................................................91Material & Methods.....................................................................................................................................94Results...........................................................................................................................................................105Discussion....................................................................................................................................................111Conclusions..................................................................................................................................................115References....................................................................................................................................................116Figures and Tables.....................................................................................................................................124
v
Chapter 5.................................................................................................................................134The Effect of Cyclic Strain on the Tensile Properties of a Naturally-Derived, Decellularized Tendon Scaffold Seeded with Allogeneic Tenocytes and Associated mRNA Expression.........................................................................................................................................134
Abstract.........................................................................................................................................................136Introduction..................................................................................................................................................137Material & Methods..................................................................................................................................140Discussion....................................................................................................................................................148Conclusions..................................................................................................................................................154Competing interests...................................................................................................................................155Acknowledgements...................................................................................................................................156References....................................................................................................................................................157Figures and Tables.....................................................................................................................................162
Summary of Doctoral Thesis..................................................................................................................167Clinical Relevance to Orthopaedic Surgery.......................................................................................169Future Direction for Research................................................................................................................171References....................................................................................................................................................172
75. Whitlock PW, Seyler TM, Northam C, Smith TL, Van Dyke ME, Poehling GG, et
al. The Effect of Cyclic Strain on the Tensile Properties of a Naturally-Derived,
Decellularized Tendon Scaffold Seeded with Allogeneic Tenocytes and Associated mRNA
Expression. J Surg Orthop Adv. 2012;Accepted for publication.
76. Cousineau-Pelletier P, Langelier E. Relative contributions of mechanical
degradation, enzymatic degradation, and repair of the extracellular matrix on the response
of tendons when subjected to under- and over- mechanical stimulations in vitro. J Orthop
Res. 2010 Feb;28(2):204-10. Epub 2009/09/03.
77. Eriksson E. Sports injuries of the knee ligaments: their diagnosis, treatment,
rehabilitation, and prevention. Med Sci Sports. 1976 Fall;8(3):133-44. Epub 1976/01/01.
48
FIGURES AND TABLES
Figure 1. Number of Pubmed listed publications on the topic of tissue engineering of ligaments. Search conducted on 04/24/2013 using the keywords “tissue engineering” and “ligament”. Thirteen publications found for the year 2013 so far which represents last data point in graph. Increased activity noted since 1999/2000.
49
Table I. Augmentation Devices for Rotator Cuff Repair
Name Material Source Comments Distributed by
Restore Small Intestinal Submucosa Porcine DePuy Orthopaedics CuffPatch Small Intestinal Submucosa Porcine cross-linked Biomet/Organogenesis GraftJacket Dermis Human Wright Medical Technology
ArthroFlex Dermis Human Arthrex Conexa Dermis Porcine α-Gal
Figure 1. Experimental flowchart detailing the total number of human Freeze-Dried
Achilles Tendon Allografts (hFDATAs) used and their subsequent processing and
analysis.
79
Figure 2. Histologic Analysis of hFDATA’s and hFDATA-derived Scaffolds. A) H&E-stained longitudinal section of hFDATA; B) DAPI-stained longitudinal section of hFDATA; C) H&E-stained section of scaffold; D) DAPI-stained section of scaffold. Panels A,C at 50X magnification. Panels B,D at 200X magnification.
80
Figure 3. Scanning Electron Microscopy of hFDATA’s and hFDATA-derived Scaffolds. A) Cross-section of hFDATA; B) Cross-section of scaffold. 150X magnification.
81
Figure 4. In Vitro Cyto-compatibility of hFDATA-derived Scaffolds: Direct Contact Method. A) Metabolic activity of cells exposed to scaffold and positive control (media alone). B) Cell viability of cells exposed to scaffold and positive control (media alone). Data expressed as mean + SEM.
82
Figure 5. Assessment of Virus-inactivating Efficacy During Production of FDATA-derived Scaffolds. Data expressed as mean + SEM.
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Figure 6. Histology of Implanted of hFDATA-derived Scaffolds at 12 Weeks. A) hFDATA 50x. B) hFDATA 200x. C) Scaffold 50x. D) Scaffold 200x. Cross-section centered on the graft tunnel with the surrounding graft-tunnel interface at the periphery of the frame, distal femur, Masson’s Trichrome stain.
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Table 1. Scoring Results for Histological Analysis of Implanted Scaffolds and hFDATA’s after 12 weeks. Data expressed as mean, (range).
Table 1. Scoring Results for Histological Analysis of Implanted Scaffolds and hFDATA’s after 12 weeks.
Table 2. Tensile Testing of hFDATA’s and hFDATA-derived Scaffolds: The ultimate tensile load at break (UTL, N), stiffness (N/mm), ultimate tensile stress at break (UTS, MPa), elastic modulus (E, MPa) % elongation at UTS (mm/mm*100) observed for the scaffolds (n=10) were 100.30% (p=0.99), 72.63% (p=0.15), 69.24% (p=0.17), 59.30% (p=0.09) and 138.47% (P=0.01) of the values observed for the hFDTAs (Mean + SEM, Student’s two-tailed t-test, equal variances).
Elastic Modulus + SEM [Mpa] 280.51 + 52.45 166.34 + 36.09 59.3 0.09 % Elongation at UTS + SEM [mm/mm*100] 23.91 + 1.57 33.11 + 2.79 138.47 0.01
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Table 3. Tensile testing of Implanted hFDATA’s and hFDATA-derived Scaffolds at 12 Weeks. The ultimate tensile load at break (UTL, N), stiffness (N/mm), ultimate tensile stress at break (UTS, MPa), elastic modulus (E, MPa) % elongation at UTS (mm/mm*100) observed for the scaffolds (n=10) were 116.16% (p=0.08), 105.24% (p=0.94), 96.75% (p=0.96), 107.94% (p=0.92) and 120.03% (P=0.66) of the values observed for the hFDTAs (Data expressed as mean + SEM, % of hFDATA. Student’s two-tailed t-test, equal variances).
Table 3. Tensile Properties In vivo Property hFDATA Scaffold % hFDATA p-value
Alpha-Gal on bioprostheses: xenograft immune response in cardiac surgery. European
123
journal of clinical investigation. 2005 Jan;35(1):17-23. PubMed PMID: 15638815. Epub
2005/01/11. eng.
124
FIGURES AND TABLES
Figure 1. Histologic Analysis of native porcine patellar tendon and porcine patellar tendon-derived scaffolds. (A) H&E-stained cross section of native patellar tendon; (B) H&E-stained cross section of scaffold; (C) Masson trichrome-stained cross section of native patellar tendon; (D) Masson trichrome-stained cross section of scaffold; (E) DAPI-stained cross section of native patellar tendon; (F) DAPI-stained cross section of scaffold. Removal of nuclear material and an increase in intra-fascicular and inter-fascicular space after oxidative treatment was observed. Panels a through F at 200X magnification.
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Figure 2. Scanning Electron Microscopy of native porcine patellar tendon and porcine patellar tendon-derived scaffolds. (A) Cross-section of native porcine patellar tendon; (B) Cross-section of porcine patellar tendon-derived scaffold. There was an increase in increase in inter-fascicular and intra-fascicular space. 50X magnification.
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Figure 3. Mercury intrusion porosimetry demonstrated a statistically significant increase in total porosimetry (A) (p<0.001) and mean pore size (B) (p<0.001) in decellularized and oxidized scaffolds compared to native tendons.
127
Figure 4. DNA detection assay revealed a significant decreased of mean DNA content in the porcine tendon-derived scaffold by 61% when compared to native tendon, p<0.001. Data expressed as the mean and standard deviation.
128
Figure 5. Within 24 hours of the oxidation step of the decellularization protocol, the peracetic acid was removed to a level below detection limits.
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Figure 6. ELISA inhibition assay performed with the monoclonal anti-Gal antibody M86. Data presented as % inhibition of M86 binding to α-gal-BSA in ELISA, after overnight incubation of M86 diluted 1/100. (A) Measuring α- gal epitope expression in native porcine patellar tendon [white circle] and porcine tendon-derived scaffold after decellularization and oxidation [black circle]. This was a reduction of approximately 66% of α-Gal epitopes expressed in the tissue at 50% inhibition of M86 binding. (B) As a standard to calculate epitope number, mouse myeloma SP2/0 cells at various concentrations were used because these cells to express approximately 106 α-gal epitopes per cell. Error bars represent standard deviation.
130
Figure 7. Tumor necrosis factor (TNF-a) (A) and IL-6 (B) release by human acute monocytic leukemia (THP-1) cells exposed to porcine tendon-derived scaffold and native tendon. Treatment of native tendon with a novel decellularization and oxidation process resulted in a significantly (p<0.001) lower production of TNF-a release compared with native tendon and LPS stimulation. Similar results were observed in IL-6 release. LPS was used as a reference in both experiments with at two concentrations (10ng/ml). Results are displayed as the mean and standard deviation for each group.
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Figure 8. ELISA measurements of anti-Gal response in monkeys who received native porcine patellar tendon and monkeys who received porcine tendon-derived scaffolds. Response is depicted as fold-change from baseline (pre-implantation) for anti-Gal antibody at 50% maximal binding There was a greater than 40% increase in titer in the initial 2 to 4 week post implantation period in the monkeys receiving native tendon as compared with the monkey engrafted with tendon scaffold. No difference was noted at the 8 and 13 week time points. Error bars represent standard deviation.
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Table 1. Mass spectrometry results listing specific mass, accession number, protein description and score per sample.
UTL = ultimate tensile load. UTS = ultimate tensile stress. Values are given as the mean and the standard deviation of the mean. P values were calculated using Student t test (tw-tailed, equal variances) or Mann-Whitney Rank Sum test if normaility failed.
134
CHAPTER 5
THE EFFECT OF CYCLIC STRAIN ON THE TENSILE PROPERTIES OF A
33. Butler DL, Grood ES, Noyes FR: Biomechanics of ligaments and tendons. Exerc
Sport Sci Rev 1978, 6: 125-181
161
34. Fung YCB. Biomechanics: Mechanical Properties of Living Tissues. New York:
Springer-Verlag; 1981:222.
35. Kear M, Smith RN: A method for recording tendon strain in sheep during
locomotion. Acta Orthop Scand 1975, 46: 896-905
36. Woo SL, Danto MI, Ohland KJ, Lee TQ, Newton PO: The use of laser micrometer
system to determine the cross-sectional shape and area of ligaments: a comparative
study with two existing methods. J Biomech Eng 1990, 112:426-431
162
FIGURES AND TABLES
Figure 1. Tensile Properties (A), Young’s modulus of UNSEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons; (C), Stiffness of UNSEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons; (E), Ultimate Tensile Stress of UNSEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons. (B), Young’s modulus of SEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons; (D), Stiffness of SEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons; (F), Ultimate Tensile Stress of SEEDED scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx) and fresh-frozen FDP tendons. Bars represent significant differences, p<0.05. Cyclic strain parameters: f=1 Hz, duration=1 hour per day, time=7 days.
163
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Figure 2. mRNA Expression Relative gene expression by cells of seeded scaffolds after 48 hour initial culture (T0 No Strain [48 hrs]), 7 days in culture in the absence of cyclic strain (T7 No Strain), 7 days in culture in the presence of cyclic strain (T7 5% Strain BioRx). (A) Collagen I, (B) Collagen III, (C) MMP-2 (matrix metalloproteinase-2), (D) Tenomodulin. Gene expression was normalized. Bars represent significant differences, p<0.05. Cyclic strain parameters: f=1 Hz, duration=1 hour per day, time=7 days.
Ultimate Tensile Stress (UTS), Young’s modulus (Y), and Stiffness (S) of fresh-frozen FDP tendons, and seeded and un-seeded scaffolds after 48 hours (T0) and 7 days (T7) in culture in the presence (5% Strain BioRx) or absence (No Strain) of cyclic strain. Bars represent significant differences, p<0.05. Cyclic strain parameters: f=1 Hz, duration=1 hour per day, time=7 days.
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CHAPTER 6
SUMMARY, CLINICAL RELEVANCE, & FUTURE DIRECTIONS
Thorsten M. Seyler, M.D.
167
SUMMARY OF DOCTORAL THESIS
Decellularized tissues have been used successfully in several areas of tissue
engineering. Numerous decellularization protocols have been utilized contributing to the
knowledge of techniques that can be used to adequately decellularize tissues.36,62,67,70 The
data presented in this thesis suggest that a novel decellularization and oxidation protocol
that combines physical, chemical, and enzymatic modalities is effective for decellularizing
tendons from various sources including chicken flexor digitorum profundus tendons,
human Achilles tendon, and pig patellar tendons.43,66,69 This protocol is effective in
reducing cellular material and lipids in general and decreasing the expression of antigenic
material such as the α-Gal epitope in xenogenic sources. Both the removal of cellular nad
nuclear material as well as the reduced expression of the α-Gal epitope may reduce the
immunogenicity of scaffolds.9,20,36,59,62 Exposure to enzymatic treatment with trypsin and
oxidation with peracetic acid disrupts cell-matrix interactions and increases total tissue
porosity and pore size without compromising biomechanical properties of the source
tissue.43,66,69 The retention of the extracellular matrix and the modified scaffold structure
facilitate re-population by host cells.66 This process is critically important because the
maintenance of the biomechanical properties allows for the slow in vivo remodeling
process while preserving function.69 The incorporation of peracetic acid and lyophilization
steps in the protocol also have shown to be effective in virus inactivation and tissue
sterilization making this protocol a potential method for the preparation of human allograft
tissue.66
168
In summary, the tissue-engineered scaffolds produced using the novel
decellularization and oxidation protocol demonstrate:
(1) Decreased DNA content and amount of cellular material leading to reduced
immunogenic potential
(2) Non-cytotoxic and biocompatible in vitro and in vivo which make it an ideal
candidate for clinical translation
(3) Alterations in the micro-architecture including increased porosity and pore size
promoting cell infiltration and re-population
(4) Preservation of tensile properties which offers rapid functionality and suitability
for clinical translation
(5) Preservation of extracellular matrix serves as a structural and logistic template
for cell attachment which also facilitates site-specific differentiation and
remodeling
(6) Can be successfully seeded with progenitor cells and subsequently may permit
formation of functional tissues
(7) Mechanical loading of seeded scaffolds improves and maintains the tensile
properties which ultimately enhances the rate of tissue development
(8) Virus inactivation and tissue sterilization reducing the risk of disease
transmission
(9) Reduction of xenoantigens such as the α-gal epitope decreasing the risk for
xenogenic tissue rejection
169
CLINICAL RELEVANCE TO ORTHOPAEDIC SURGERY
Tendon and ligament injuries place a significant burden on the United States
economy, accounting for nearly 32 million orthopaedic musculoskeletal injuries
annually.50 These ligamentous and tendinous injuries include tears to the rotator cuff and
anterior cruciate ligament; tendon injuries of the hand and foot; and damage to the
medial/lateral collateral ligament of the elbow. The goals of orthopaedic surgery are to
restore normal joint motion, reduce pain, return patients to full function, and prevent further
degenerative disease. Despite many recent surgical advances, surgeons continue to seek
alternative reconstruction techniques and graft choices in an attempt to meet the patient’s
functional demands, reduce complications, and improve clinical outcomes.
The popularity and use of musculoskeletal allograft tissue in a wide variety of
orthopaedic procedures has dramatically increased over the last 20 years. In a statement
from 2011, the American Academy of Orthopaedic Surgeons (AAOS) believes that for
appropriate patients, musculoskeletal allografts represent a therapeutic alternative.61
Potential advantages of allografts include decreased donor site morbidity, immediate
availability of various graft sizes (off-the-shelf), less postoperative pain, better
postoperative function, and lower incidence of postoperative arthrofibrosis.
The greatest disadvantage of allografts is the potential risk of disease transmission.
Contamination of allografts can occur while the tissue remains confined to the donor’s
corpus and during or after tissue removal because of improper tissue processing
procedures.46,68
In the past, the two methods employed to avoid disease transmission were graft
sterilization using ethylene oxide treatment or gamma irradiation. Ethylene oxide is no
170
longer used because of associated post-operative synovitis and intra-articular graft
destruction. Gamma irradiation is thought to create free radicals and modify nucleic acids
leading to virus and bacterial destruction. However, it has been also associated with
disruption of the structural integrity of the graft.24 Initial concerns of graft weakness
associated with using ethylene oxide or high dose gamma irradiation sterilization have been
addressed by employing a multimodal process that involves sterile harvesting of tissue
from the donor, rigorous donor screening/testing, antibiotic soaking, low dose irradiation
(1.2-1.8 Mrad), and use of a final preservation technique such as lyophilization or
freezing.32,34 Despite these advances in graft sterilization, one of the major drawbacks of
using allografts is slow incorporation into host bone and graft remodeling, which may lead
to reduced graft strength resulting in early failure.11
Naturally derived extracellular-matrix scaffolds have evolved as a viable option for
tendon and ligament repair21. However, some materials, such as small intestinal
submucosa, lack the initial strength to adequately replace tissue structures; instead they
have been utilized successfully to augment soft tissue repairs.16,35,44,49 A decellularization
and oxidation protocol is presented that can produce a tissue-engineered scaffold that
possesses mechanical properties similar to the native tissue and can maintain its mechanical
properties until remodeling has occurred and improved functionality. Application of this
procedure may allow patients to have an expedited return to work and recreational activities
after undergoing orthopaedic ligament and tendon reconstruction surgery.
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FUTURE DIRECTION FOR RESEARCH
Our research efforts are centered on addressing clinical problems, and ultimately,
to improve patient care by translating this basic-science knowledge into clinical practice.
Tendon and ligament injuries pose a significant clinical problem that is inadequately
addressed by current orthopaedic interventions. Tissue-engineered scaffolds offer a
promising alternative to current graft options by delivering a graft with favorable
mechanical properties to the site of injury, providing functional utilization of reconstructed
tendons and ligaments immediately after surgery, as well as graft integration and healing
in the months to years following surgery. The experiments in this thesis demonstrated that
a novel decellularization and oxidation protocol that combines physical, chemical, and
enzymatic modalities can be used to produce naturally derived scaffolds with significant
clinical potential. Research using a small animal study and an ectopic model of
xenotransplantation in hon-human primates has been successful. Although this represents
a critical step in building a foundation for future studies and demonstrating the feasibility
of using a tissue-engineered scaffold for ligament and tendon reconstruction, several
challenges must be addressed prior to translation of this method into the clinic. Among
these future studies, scaffold processing and exploration of tissue sources are among the
most important variables that impact the host response and remodeling outcome. For
clinical translation, many hurdles remain to be overcome such a defining the optimal donor
source and addressing the key concern when using xenograft tissues; immunogenicity and
its impact on graft rejection and long-term graft survival.
172
REFERENCES
1. Weiss, A. B.; Blazina, M. E.; Goldstein, A. R.; and Alexander, H.: Ligament
replacement with an absorbable copolymer carbon fiber scaffold--early clinical experience.
Clin Orthop Relat Res, (196): 77-85, 1985.
2. Bolton, C. W., and Bruchman, W. C.: The GORE-TEX expanded
polytetrafluoroethylene prosthetic ligament. An in vitro and in vivo evaluation. Clin Orthop
Relat Res, (196): 202-13, 1985.
3. Nikolaou, P. K.; Seaber, A. V.; Glisson, R. R.; Ribbeck, B. M.; and Bassett, F. H.,
70. Arenas-Herrera, J. E.; Ko, I. K.; Atala, A.; and Yoo, J. J.: Decellularization for
whole organ bioengineering. Biomed Mater, 8(1): 014106, 2013.
182
CURRICULUM VITAE
Thorsten M. Seyler, M.D.
EDUCATION
Wake Forest University, Graduate School of Arts and Sciences, Winston-Salem, NC Ph.D., Molecular Medicine and Translational Science Graduate Program: 2007 – 2012
Emory University School of Medicine, Atlanta, GA Visiting Student, International Exchange Scholarship Program, Ruprecht-Karls-University, School of Medicine, 2004
Mayo Medical and Graduate School of Medicine, Rochester MN Visiting Student, International Exchange Scholarship Program, Ruprecht-Karls-University, School of Medicine, 2002-2003
Ruprecht-Karls-University, School of Medicine, Heidelberg, Germany M.D., 2000 – 2005 Semmelweis University for Medical Science, Budapest, Hungary
Premedical Sciences, 1998 – 2000
183
POST GRADUATE EDUCATION & TRAINING
Wake Forest University, Department of Orthopaedic Surgery, Winston-Salem, NC Resident, Orthopaedic Surgery: July 2007 – present Program Director: Jason E. Lang, M.D. Wake Forest University, Department of General Surgery, Winston-Salem, NC
Resident (Intern), General Surgery: July 2009 – June 2010 Program Director: Jason E. Lang, M.D. Wake Forest University, Graduate School of Arts and Sciences, Winston-Salem,
NC Graduate Student (Ph.D.), Molecular Medicine and Translational Science Graduate Program: August 2007 – December 2012 Program Directors: Richard F. Loeser, Jr., M.D. & Kathleen B. Brosnihan, Ph.D.
Sinai Hospital of Baltimore, Rubin Institute for Advanced Orthopedics, Baltimore, MD Research Fellow, Center for Joint Preservation and Reconstruction: July 2005 – June 2007 Program Director: Michael A. Mont, M.D.
184
MEDICAL LICENSURES
Resident training license, State of North Carolina, 2007 (active) Full license, Federal Republic of Germany, 2005 (active)
185
PROFESSIONAL SOCIETY MEMBERSHIPS
Orthopaedic Research Society: Associate Member 007 - present Southern Orthopaedic Association: Resident Member
2007 - present American Academy of Orthopaedic Surgeons: Resident Member
2007 - present American Association of Hip and Knee Surgeons: Resident Member
2012 - present
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HONORS & AWARDS
2003 Nominated for the ACR/ARHP Student Achievement Award at the 67th American College of Rheumatology Annual Scientific Meeting, Orlando, FL (Lead-author)
2006 Award for Best Resident/Fellow Clinical Research Paper, 65th Annual Meeting Maryland Orthopaedic Association, Baltimore, MD (Co-author)
2008 Award for Best Basic Science Research Paper, Resident Research Day, Department of Orthopaedic Surgery, Wake Forest University School of Medicine, Winston-Salem, NC (Lead-author)
2009 Award of Excellence for Scientific Exhibits, 76th Annual Meeting of the American Academy of Orthopaedic Surgeons, Las Vegas, NV (Co-author)
2012 Runner-up Best Clinical Science Resident Research Paper, 32nd Annual Oscar Miller Day Symposium, Charlotte, NC (Lead-author)
2012 Best Clinical Science Resident Research Paper, 32nd Annual Oscar Miller Day Symposium, Charlotte, NC (Co-author)
2012 Best Research Paper, 100th Annual Meeting of The Clinical Orthopaedic Society (COS), The Palmer House, Chicago, IL (Co-author)
2012 Best Research Paper, Annual Meeting North Carolina Orthopaedic Association, Pinehurst Resort, Village of Pinehurst, NC (Co-author)
187
EDITORIAL ACTIVITIES
Editorial Advisory Board Member, Open Bone Journal 2009 - present
Associate Editor, Bone & Joint Research 2012 - present
Consultant Reviewer, Clinical Orthopaedics and Related Research 2008 - present
Consultant Reviewer, Journal of Joint and Bone Surgery Am. Vol. 2006 - present
Consultant Reviewer, Journal of Arthroplasty 2006 - present
Guest Editor, Proceedings of the 1st Annual United States Comprehensive Course on Total Hip Resurfacing Arthroplasty, Journal of Bone and Joint Surgery Am. Vol., Volume 90-A, Supplement 3, Pages 1-148, 2008
Guest Editor, Proceedings of the 2nd Annual United States Comprehensive Course on Total Hip Resurfacing Arthroplasty, Bulletin of the NYU Hospital for Joint Diseases, Volume 62 (2), Pages 101-192, 2009
Guest Editor, Symposium on Advanced Techniques for Rehabilitation after Total Hip and Knee Arthroplasty, Clinical Orthopaedics and Related Research, Volume 476 (6), Pages 1395-1500, 2009
188
COMMITTEES
Program Director, First Annual U.S. Comprehensive Course on Total Hip Resurfacing Arthroplasty, November 8-10, 2007, Annapolis, MD
Program Director, Second Annual U.S. Comprehensive Course on Total Hip Arthroplasty, October 24-25, 2008, Los Angeles, CA
Program Director, Third Annual U.S. Comprehensive Course on Total Hip Arthroplasty, September 4-5, 2009, Baltimore, MD
Program Director, Fourth Annual Hip Joint Course, Treatment for Young Patients with Hip Arthritis, August 27-29, 2010, Baltimore, MD
Program Director, Sixth Annual Joint Course, New Technology for the Treatment of Adult Hip and Knee Disorders, August 24-25, 2012, Baltimore, MD
Voting Delegate, International Consensus Meeting on Periprosthetic Joint Infection, August 1st, 2013, Philadelpia, PA
189
PEER-REVIEWED PUBLICATIONS
1. Marulanda, G. A.; Ragland, P. S.; Seyler, T. M.; and Mont, M. A.: Reductions in blood loss with use of a bipolar sealer for hemostasis in primary total knee arthroplasty. Surg Technol Int, 14: 281-6, 2005.
2. Seyler, T. M.; Bonutti, P. M.; Ragland, P. S.; Marulanda, G. A.; and Mont, M. A.: Minimally invasive lateral approach to total knee arthroplasty. Seminars in Arthroplasty, 16(3): 223-226, 2005.
3. Weyand, C. M.; Seyler, T. M.; and Goronzy, J. J.: B cells in rheumatoid synovitis. Arthritis Res Ther, 7 Suppl 3: S9-12, 2005.
4. Park, Y. W.; Pryshchep, S.; Seyler, T. M.; Goronzy, J. J.; and Weyand, C. M.: B cells as a therapeutic target in autoimmune diseases. Expert Opin Ther Targets, 9(3): 431-45, 2005.
5. Seyler, T. M.; Park, Y. W.; Takemura, S.; Bram, R. J.; Kurtin, P. J.; Goronzy, J. J.; and Weyand, C. M.: BLyS and APRIL in rheumatoid arthritis. J Clin Invest, 115(11): 3083-92, 2005.
6. Mont, M. A.; Bonutti, P. M.; Seyler, T. M.; Plate, J. F.; Delanois, R. E.; and Kester, M.: The Future of High Performance Total Knee Arthroplasty. Seminars in Arthroplasty, 17(2): 80-87, 2006.
7. Mont, M. A.; Bonutti, P. M.; Seyler, T. M.; Plate, J. F.; Delanois, R. E.; and Kester, M.: The Future of High Performance Total Hip Arthroplasty. Seminars in Arthroplasty, 17(2): 88-92, 2006.
8. Seyler, T. M.; Etienne, G.; Plate, J. F.; Fisher, P.; and Mont, M. A.: Use of modular large femoral heads without liners in hip arthroplasty. Surg Technol Int, 15: 217-20, 2006.
9. Seyler, T. M.; Marulanda, G. A.; Delanois, R. E.; and Mont, M. A.: Limited Approach Surface Replacement Total Hip Arthroplasty. Oper Tech Orthop, 16(2): 120-125, 2006.
10. Seyler, T. M.; Mont, M. A.; Ragland, P. S.; Kachwala, M. M.; and Delanois, R. E.: Sports activity after total hip and knee arthroplasty: specific recommendations concerning tennis. Sports Med, 36(7): 571-83, 2006.
11. Mont, M. A.; Ragland, P. S.; Etienne, G.; Seyler, T. M.; and Schmalzried, T. P.: Hip resurfacing arthroplasty. J Am Acad Orthop Surg, 14(8): 454-63, 2006.
12. Leadbetter, W. B.; Seyler, T. M.; Ragland, P. S.; and Mont, M. A.: Indications, contraindications, and pitfalls of patellofemoral arthroplasty. J Bone Joint Surg Am, 88 Suppl 4: 122-37, 2006.
13. Diaz-Borjon, A.; Seyler, T. M.; Chen, N. L.; and Lim, S. S.: Bisphosphonate-associated arthritis. J Clin Rheumatol, 12(3): 131-3, 2006.
14. Marulanda, G.; Seyler, T. M.; Sheikh, N. H.; and Mont, M. A.: Percutaneous drilling for the treatment of secondary osteonecrosis of the knee. J Bone Joint Surg Br, 88(6): 740-6, 2006.
15. Bonutti, P. M.; Seyler, T. M.; Kester, M.; McMahon, M.; and Mont, M. A.: Minimally invasive revision total knee arthroplasty. Clin Orthop Relat Res, 446: 69-75, 2006.
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16. Mont, M. A.; Seyler, T. M.; Marulanda, G. A.; Delanois, R. E.; and Bhave, A.: Surgical treatment and customized rehabilitation for stiff knee arthroplasties. Clin Orthop Relat Res, 446: 193-200, 2006.
17. Bonutti, P. M.; Seyler, T. M.; Delanois, R. E.; McMahon, M.; McCarthy, J. C.; and Mont, M. A.: Osteonecrosis of the knee after laser or radiofrequency-assisted arthroscopy: treatment with minimally invasive knee arthroplasty. J Bone Joint Surg Am, 88 Suppl 3: 69-75, 2006.
18. Mont, M. A.; Seyler, T. M.; Marker, D. R.; Marulanda, G. A.; and Delanois, R. E.: Use of metal-on-metal total hip resurfacing for the treatment of osteonecrosis of the femoral head. J Bone Joint Surg Am, 88 Suppl 3: 90-7, 2006.
19. Mont, M. A.; Seyler, T. M.; Plate, J. F.; Delanois, R. E.; and Parvizi, J.: Uncemented total hip arthroplasty in young adults with osteonecrosis of the femoral head: a comparative study. J Bone Joint Surg Am, 88 Suppl 3: 104-9, 2006.
20. Seyler, T. M.; Bonutti, P. M.; Shen, J.; Naughton, M.; and Kester, M.: Use of an alumina-on-alumina bearing system in total hip arthroplasty for osteonecrosis of the hip. J Bone Joint Surg Am, 88 Suppl 3: 116-25, 2006.
21. Mont, M. A.; Marulanda, G. A.; Seyler, T. M.; Plate, J. F.; and Delanois, R. E.: Core decompression and nonvascularized bone grafting for the treatment of early stage osteonecrosis of the femoral head. Instr Course Lect, 56: 213-20, 2007.
22. Akbar, M.; Seyler, T. M.; Abel, R.; and Gerner, H. J.: [Heterotope Ossifikation bei Querschnittlähmung und Schädel-Hirn-Trauma]. Phys Rehab Kur Med, 17(3): 156-171, 2007.
23. Seyler, T. M.; Cui, Q.; Mihalko, W. M.; Mont, M. A.; and Saleh, K. J.: Advances in hip arthroplasty in the treatment of osteonecrosis. Instr Course Lect, 56: 221-33, 2007.
24. Mont, M. A.; Jones, L. C.; Seyler, T. M.; Marulanda, G. A.; Saleh, K. J.; and Delanois, R. E.: New treatment approaches for osteonecrosis of the femoral head: an overview. Instr Course Lect, 56: 197-212, 2007.
25. Childress III, L.; Ulrich, S. D.; Seyler, T. M.; Delanois, R. E.; Marker, D. R.; and Mont, M. A.: Role of Metal-on-Metal Resurfacing in the Treatment of Late Stage Osteonecrosis. Seminars in Arthroplasty, 18(3): 216-219, 2007.
26. Diaz Jr, L. A.; Foss, C. A.; Thornton, K.; Nimmagadda, S.; Endres, C. J.; Uzuner, O.; Seyler, T. M.; Ulrich, S. D.; Conway, J.; Bettegowda, C.; Agrawal, N.; Cheong, I.; Zhang, X.; Ladenson, P. W.; Vogelstein, B. N.; Mont, M. A.; Zhou, S.; Kinzler, K. W.; Vogelstein, B.; and Pomper, M. G.: Imaging of musculoskeletal bacterial infections by [124I]FIAU-PET/CT. PLoS ONE, 2(10), 2007.
27. Delanois, R. E.; Seyler, T. M.; Essner, A.; Schmidig, G.; and Mont, M. A.: Cementation of a polyethylene liner into a metal shell. J Arthroplasty, 22(5): 732-7, 2007.
28. Boyd, H. S.; Ulrich, S. D.; Seyler, T. M.; Marulanda, G. A.; and Mont, M. A.: Resurfacing for Perthes disease: an alternative to standard hip arthroplasty. Clin Orthop Relat Res, 465: 80-5, 2007.
29. Mont, M. A.; Seyler, T. M.; Ulrich, S. D.; Beaule, P. E.; Boyd, H. S.; Grecula, M. J.; Goldberg, V. M.; Kennedy, W. R.; Marker, D. R.; Schmalzried, T. P.; Sparling, E. A.; Vail, T. P.; and Amstutz, H. C.: Effect of changing indications and techniques on total hip resurfacing. Clin Orthop Relat Res, 465: 63-70, 2007.
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30. Mont, M. A.; Seyler, T. M.; Ragland, P. S.; Starr, R.; Erhart, J.; and Bhave, A.: Gait analysis of patients with resurfacing hip arthroplasty compared with hip osteoarthritis and standard total hip arthroplasty. J Arthroplasty, 22(1): 100-8, 2007.
31. Mont, M. A.; Marker, D. R.; Seyler, T. M.; Gordon, N.; Hungerford, D. S.; and Jones, L. C.: Knee arthroplasties have similar results in high- and low-activity patients. Clin Orthop Relat Res, 460: 165-73, 2007.
32. Ulrich, S. D.; Mont, M. A.; Bonutti, P. M.; Seyler, T. M.; Marker, D. R.; and Jones, L. C.: Scientific evidence supporting computer-assisted surgery and minimally invasive surgery for total knee arthroplasty. Expert Rev Med Devices, 4(4): 497-505, 2007.
33. Mont, M. A.; Ulrich, S. D.; and Seyler, T. M.: Role of thrombotic and fibrinolytic alterations in the pathogenesis and treatment of osteonecrosis. J Rheumatol, 34(3): 466-8, 2007.
34. Ulrich, S. D.; Bhave, A.; Marker, D. R.; Seyler, T. M.; and Mont, M. A.: Focused rehabilitation treatment of poorly functioning total knee arthroplasties. Clin Orthop Relat Res, 464: 138-45, 2007.
35. Ulrich, S. D.; Bonutti, P. M.; Seyler, T. M.; Marker, D. R.; Jones, L. C.; and Mont, M. A.: Outcomes-based evaluations supporting computer-assisted surgery and minimally invasive surgery for total hip arthroplasty. Expert Rev Med Devices, 4(6): 873-83, 2007.
36. Seyler, T. M.; Bonutti, P. M.; Ulrich, S. D.; Fatscher, T.; Marker, D. R.; and Mont, M. A.: Minimally invasive lateral approach to total knee arthroplasty. J Arthroplasty, 22(7 Suppl 3): 21-6, 2007.
37. Marker, D. R.; Seyler, T. M.; Jinnah, R. H.; Delanois, R. E.; Ulrich, S. D.; and Mont, M. A.: Femoral neck fractures after metal-on-metal total hip resurfacing: a prospective cohort study. J Arthroplasty, 22(7 Suppl 3): 66-71, 2007.
38. Seyler, T. M.; Marker, D. R.; Bhave, A.; Plate, J. F.; Marulanda, G. A.; Bonutti, P. M.; Delanois, R. E.; and Mont, M. A.: Functional problems and arthrofibrosis following total knee arthroplasty. J Bone Joint Surg Am, 89 Suppl 3: 59-69, 2007.
39. Akbar, M.; Abel, R.; Seyler, T. M.; Bedke, J.; Haferkamp, A.; Gerner, H. J.; and Mohring, K.: Repeated botulinum-A toxin injections in the treatment of myelodysplastic children and patients with spinal cord injuries with neurogenic bladder dysfunction. BJU Int, 100(3): 639-45, 2007.
40. Bhave, A.; Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; Plate, J. F.; and Mont, M. A.: Functional problems and treatment solutions after total hip arthroplasty. J Arthroplasty, 22(6 Suppl 2): 116-24, 2007.
41. Kolisek, F. R.; Seyler, T. M.; Ulrich, S. D.; Marker, D. R.; Jessup, N. M.; and Mont, M. A.: A Comparison of the Minimally Invasive Dual-Incision versus Posterolateral Approach in Total Hip Arthroplasty. Surg Technol Int, 17: 253-8, 2008.
42. Mont, M. A.; Ulrich, S. D.; Seyler, T. M.; Smith, J. M.; Marker, D. R.; McGrath, M. S.; Hungerford, D. S.; and Jones, L. C.: Bone scanning of limited value for diagnosis of symptomatic oligofocal and multifocal osteonecrosis. J Rheumatol, 35(8): 1629-34, 2008.
43. Seyler, T. M.; Lai, L. P.; Sprinkle, D. I.; Ward, W. G.; and Jinnah, R. H.: Does computer-assisted surgery improve accuracy and decrease the learning curve in hip resurfacing? A radiographic analysis. J Bone Joint Surg Am, 90 Suppl 3: 71-80, 2008.
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44. Bonutti, P. M.; McGrath, M. S.; Ulrich, S. D.; McKenzie, S. A.; Seyler, T. M.; and Mont, M. A.: Static progressive stretch for the treatment of knee stiffness. Knee, 15(4): 272-6, 2008.
45. Mont, M. A.; McGrath, M. S.; Ulrich, S. D.; Seyler, T. M.; Marker, D. R.; and Delanois, R. E.: Metal-on-metal total hip resurfacing arthroplasty in the presence of extra-articular deformities or implants. J Bone Joint Surg Am, 90 Suppl 3: 45-51, 2008.
46. McGrath, M. S.; Desser, D. R.; Ulrich, S. D.; Seyler, T. M.; Marker, D. R.; and Mont, M. A.: Total hip resurfacing in patients who are sixty years of age or older. J Bone Joint Surg Am, 90 Suppl 3: 27-31, 2008.
47. Robinson, Y.; Heyde, C. E.; Tschoke, S. K.; Mont, M. A.; Seyler, T. M.; and Ulrich, S. D.: Evidence supporting the use of bone morphogenetic proteins for spinal fusion surgery. Expert Rev Med Devices, 5(1): 75-84, 2008.
48. Delanois, R. E.; McGrath, M. S.; Ulrich, S. D.; Marker, D. R.; Seyler, T. M.; Bonutti, P. M.; and Mont, M. A.: Results of total knee replacement for isolated patellofemoral arthritis: when not to perform a patellofemoral arthroplasty. Orthop Clin North Am, 39(3): 381-8, vii, 2008.
49. Akbar, M.; Mont, M. A.; Heisel, C.; Marker, D. R.; Ulrich, S. D.; and Seyler, T. M.: [Resurfacing for osteonecrosis of the femoral head]. Orthopade, 37(7): 672-8, 2008.
50. Bonutti, P. M.; Seyler, T. M.; Bianco, P. D.; Ulrich, S. D.; and Mont, M. A.: Inventing in orthopaedics: from idea to marketed device. J Bone Joint Surg Am, 90(6): 1385-92, 2008.
51. Marulanda, G. A.; Ulrich, S. D.; Seyler, T. M.; Delanois, R. E.; and Mont, M. A.: Reductions in blood loss with a bipolar sealer in total hip arthroplasty. Expert Rev Med Devices, 5(2): 125-31, 2008.
52. Stiehl, J. B.; Ulrich, S. D.; Seyler, T. M.; Bonutti, P. M.; Marker, D. R.; and Mont, M. A.: Bone morphogenetic proteins in total hip arthroplasty, osteonecrosis and trauma surgery. Expert Rev Med Devices, 5(2): 231-8, 2008.
53. Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; Srivastava, S.; and Mont, M. A.: Do modern techniques improve core decompression outcomes for hip osteonecrosis? Clin Orthop Relat Res, 466(5): 1093-103, 2008.
54. Seyler, T. M.; Marker, D. R.; Ulrich, S. D.; Fatscher, T.; and Mont, M. A.: Nonvascularized bone grafting defers joint arthroplasty in hip osteonecrosis. Clin Orthop Relat Res, 466(5): 1125-32, 2008.
55. Bonutti, P. M.; Dethmers, D.; Ulrich, S. D.; Seyler, T. M.; and Mont, M. A.: Computer navigation-assisted versus minimally invasive TKA: benefits and drawbacks. Clin Orthop Relat Res, 466(11): 2756-62, 2008.
56. Marker, D. R.; Seyler, T. M.; McGrath, M. S.; Delanois, R. E.; Ulrich, S. D.; and Mont, M. A.: Treatment of early stage osteonecrosis of the femoral head. J Bone Joint Surg Am, 90 Suppl 4: 175-87, 2008.
57. McGrath, M. S.; Ulrich, S. D.; Bonutti, P. M.; Smith, J. M.; Seyler, T. M.; and Mont, M. A.: Evaluation of static progressive stretch for the treatment of wrist stiffness. J Hand Surg [Am], 33(9): 1498-504, 2008.
58. Seyler, T. M.; Smith, B. P.; Marker, D. R.; Ma, J.; Shen, J.; Smith, T. L.; Mont, M. A.; Kolaski, K.; and Koman, L. A.: Botulinum neurotoxin as a therapeutic modality
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in orthopaedic surgery: more than twenty years of experience. J Bone Joint Surg Am, 90 Suppl 4: 133-45, 2008.
59. Sikes, C. V.; Lai, L. P.; Schreiber, M.; Mont, M. A.; Jinnah, R. H.; and Seyler, T. M.: Instability after total hip arthroplasty: treatment with large femoral heads vs constrained liners. J Arthroplasty, 23(7 Suppl): 59-63, 2008.
60. Ulrich, S. D.; Seyler, T. M.; Bennett, D.; Delanois, R. E.; Saleh, K. J.; Thongtrangan, I.; Kuskowski, M.; Cheng, E. Y.; Sharkey, P. F.; Parvizi, J.; Stiehl, J. B.; and Mont, M. A.: Total hip arthroplasties: what are the reasons for revision? Int Orthop, 32(5): 597-604, 2008.
61. Mont, M. A.; Marker, D. R.; Seyler, T. M.; Jones, L. C.; Kolisek, F. R.; and Hungerford, D. S.: High-impact sports after total knee arthroplasty. J Arthroplasty, 23(6 Suppl 1): 80-4, 2008.
62. Seyler, T. M.; Jinnah, R. H.; Koman, L. A.; Marker, D. R.; Mont, M. A.; Ulrich, S. D.; and Bhave, A.: Botulinum toxin type A injections for the management of flexion contractures following total knee arthroplasty. J Surg Orthop Adv, 17(4): 231-8, 2008.
63. Mont, M. A.; Schmalzried, T. P.; Zywiel, M. G.; McGrath, M. S.; and Seyler, T. M.: Perceptions concerning hip resurfacing from attendees at the Second Annual U.S. Comprehensive Course on Total Hip Resurfacing Arthroplasty. Bull NYU Hosp Jt Dis, 67(2): 102-7, 2009.
64. Bhave, A.; Zywiel, M. G.; Ulrich, S. D.; McGrath, M. S.; Seyler, T. M.; Marker, D. R.; Delanois, R. E.; and Mont, M. A.: Botulinum toxin type A injections for the management of muscle tightness following total hip arthroplasty: a case series. J Orthop Surg Res, 4: 34, 2009.
65. Marker, D. R.; Mont, M. A.; Seyler, T. M.; McGrath, M. S.; Kolisek, F. R.; and Bonutti, P. M.: Does functional improvement following TKA correlate to increased sports activity? Iowa Orthop J, 29: 11-6, 2009.
66. Shields, J. S.; Seyler, T. M.; Maguire, C.; and Jinnah, R. H.: Computer-assisted navigation in hip resurfacing arthroplasty - a single-surgeon experience. Bull NYU Hosp Jt Dis, 67(2): 164-7, 2009.
67. Kolisek, F. R.; McGrath, M. S.; Marker, D. R.; Jessup, N.; Seyler, T. M.; Mont, M. A.; and Lowry Barnes, C.: Posterior-stabilized versus posterior cruciate ligament-retaining total knee arthroplasty. Iowa Orthop J, 29: 23-7, 2009.
68. Seyler, T. M.; Mont, M. A.; Lai, L. P.; Xie, J.; Marker, D. R.; Zywiel, M. G.; and Bonutti, P. M.: Mid-term results and factors affecting outcome of a metal-backed unicompartmental knee design: a case series. J Orthop Surg Res, 4: 39, 2009.
69. Maguire, C. M.; Seyler, T. M.; Boyd, H. S.; Lai, L. P.; Delanois, R. E.; and Jinnah, R. H.: Hip resurfacing-keys to success. Bull NYU Hosp Jt Dis, 67(2): 142-5, 2009.
70. Strassmair, M.; Mont, M. A.; Seyler, T. M.; Bosebeck, H.; Marker, D. R.; and Laporte, D. M.: The use of a type-I lyophilisate collagen as an osteoinductive factor in pseudarthroses of the forearm. Surg Technol Int, 18: 213-8, 2009.
71. Zywiel, M. G.; McGrath, M. S.; Seyler, T. M.; Marker, D. R.; Bonutti, P. M.; and Mont, M. A.: Osteonecrosis of the knee: a review of three disorders. Orthop Clin North Am, 40(2): 193-211, 2009.
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72. Marker, D. R.; Mont, M. A.; Seyler, T. M.; Laporte, D. M.; and Frassica, F. J.: Current literature: an educational tool to study osteonecrosis for the orthopaedic in-training examination? Orthop Clin North Am, 40(2): 299-304, 2009.
73. Bonutti, P. M.; Zywiel, M. G.; Seyler, T. M.; Lee, S. Y.; McGrath, M. S.; Marker, D. R.; and Mont, M. A.: Minimally invasive total knee arthroplasty using the contralateral knee as a control group: a case-control study. Int Orthop, 2009.
74. Marulanda, G. A.; Krebs, V. E.; Bierbaum, B. E.; Goldberg, V. M.; Ries, M.; Ulrich, S. D.; Seyler, T. M.; and Mont, M. A.: Hemostasis using a bipolar sealer in primary unilateral total knee arthroplasty. Am J Orthop (Belle Mead NJ), 38(12): E179-83, 2009.
75. Marulanda, G. A.; Minniti, C. P.; Ulrich, S. D.; Seyler, T. M.; and Mont, M. A.: Perioperative management for orthopaedic patients with sickle cell anaemia. J Orthop Surg (Hong Kong), 17(3): 346-50, 2009.
76. Kolisek, F. R.; Mont, M. A.; Seyler, T. M.; Marker, D. R.; Jessup, N. M.; Siddiqui, J. A.; Monesmith, E.; and Ulrich, S. D.: Total knee arthroplasty using cementless keels and cemented tibial trays: 10-year results. Int Orthop, 33(1): 117-21, 2009.
77. Saenz, C. L.; McGrath, M. S.; Marker, D. R.; Seyler, T. M.; Mont, M. A.; and Bonutti, P. M.: Early failure of a unicompartmental knee arthroplasty design with an all-polyethylene tibial component. Knee, 17(1): 53-6, 2009.
78. McGrath, M. S.; Suda, A. J.; Bonutti, P. M.; Zywiel, M. G.; Marker, D. R.; Seyler, T. M.; and Mont, M. A.: Techniques for managing anatomic variations in primary total knee arthroplasty. Expert Rev Med Devices, 6(1): 75-93, 2009.
79. McGrath, M. S.; Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; and Mont, M. A.: Surface replacement is comparable to primary total hip arthroplasty. Clin Orthop Relat Res, 467(1): 94-100, 2009.
80. Harreld, K. L.; Marulanda, G. A.; Ulrich, S. D.; Marker, D. R.; Seyler, T. M.; and Mont, M. A.: Small-diameter percutaneous decompression for osteonecrosis of the shoulder. Am J Orthop (Belle Mead NJ), 38(7): 348-54, 2009.
81. Mont, M. A., and Seyler, T. M.: Advanced techniques for rehabilitation after total hip and knee arthroplasty. Clin Orthop Relat Res, 467(6): 1395, 2009.
82. Mont, M. A.; McGrath, M. S.; Bonutti, P. M.; Ulrich, S. D.; Marker, D. R.; Seyler, T. M.; and Suda, A. J.: Anatomic and physiologic rationale for various technologies for primary total hip arthroplasty. Expert Rev Med Devices, 6(2): 169-86, 2009.
83. Ulrich, S. D.; Bonutti, P. M.; Seyler, T. M.; Marker, D. R.; Morrey, B. F.; and Mont, M. A.: Restoring range of motion via stress relaxation and static progressive stretch in posttraumatic elbow contractures. J Shoulder Elbow Surg, 19(2): 196-201, 2009.
84. Akbar, M.; Bresch, B.; Seyler, T. M.; Wenz, W.; Bruckner, T.; Abel, R.; and Carstens, C.: Management of orthopaedic sequelae of congenital spinal disorders. J Bone Joint Surg Am, 91 Suppl 6: 87-100, 2009.
85. Marker, D. R.; LaPorte, D. M.; Seyler, T. M.; Ulrich, S. D.; McGrath, M. S.; Frassica, F. J.; and Mont, M. A.: Orthopaedic journal publications and their role in the preparation for the orthopaedic in-training examination. J Bone Joint Surg Am, 91 Suppl 6: 59-66, 2009.
86. Seyler, T. M.; Marker, D. R.; Boyd, H. S.; Zywiel, M. G.; McGrath, M. S.; and Mont, M. A.: Preoperative evaluation to determine candidates for metal-on-metal hip resurfacing. J Bone Joint Surg Am, 91 Suppl 6: 32-41, 2009.
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87. Marker, D. R.; Seyler, T. M.; Bhave, A.; Zywiel, M. G.; and Mont, M. A.: Does commitment to rehabilitation influence clinical outcome of total hip resurfacing arthroplasty? J Orthop Surg Res, 5: 20, 2010.
88. Marker, D. R.; Zywiel, M. G.; Johnson, A. J.; Seyler, T. M.; and Mont, M. A.: Are component positioning and prosthesis size associated with hip resurfacing failure? BMC Musculoskelet Disord, 11: 227, 2010.
89. Akbar, M.; Almatrod, S.; Furstenberg, C. H.; Hemmer, S.; Kretzer, J. P.; Abel, R.; Seyler, T. M.; Bruckner, T.; Carstens, C.; and Wiedenhofer, B.: [Kyphectomy in myelomeningocele patients. Longterm results, complications and risk analysis]. Orthopade, 39(8): 792-800, 2010.
90. Clohisy, J. C.; Oryhon, J. M.; Seyler, T. M.; Wells, C. W.; Liu, S. S.; Callaghan, J. J.; and Mont, M. A.: Function and fixation of total hip arthroplasty in patients 25 years of age or younger. Clin Orthop Relat Res, 468(12): 3207-13, 2010.
91. Akbar, M.; Balean, G.; Brunner, M.; Seyler, T. M.; Bruckner, T.; Munzinger, J.; Grieser, T.; Gerner, H. J.; and Loew, M.: Prevalence of rotator cuff tear in paraplegic patients compared with controls. J Bone Joint Surg Am, 92(1): 23-30, 2010.
92. Marulanda, G. A.; McGrath, M. S.; Ulrich, S. D.; Seyler, T. M.; Delanois, R. E.; and Mont, M. A.: Percutaneous drilling for the treatment of atraumatic osteonecrosis of the ankle. J Foot Ankle Surg, 49(1): 20-4, 2010.
93. Bonutti, P. M.; Zywiel, M. G.; Ulrich, S. D.; Stroh, D. A.; Seyler, T. M.; and Mont, M. A.: A comparison of subvastus and midvastus approaches in minimally invasive total knee arthroplasty. J Bone Joint Surg Am, 92(3): 575-82, 2010.
94. LaPorte, D. M.; Marker, D. R.; Seyler, T. M.; Mont, M. A.; and Frassica, F. J.: Educational resources for the Orthopedic In-Training Examination. J Surg Educ, 67(3): 135-8, 2010.
95. Akbar, M.; Bresch, B.; Raiss, P.; Furstenberg, C. H.; Bruckner, T.; Seyler, T. M.; Carstens, C.; and Abel, R.: Fractures in myelomeningocele. J Orthop Traumatol, 11(3): 175-82, 2010.
96. Maguire, C. M.; Seyler, T. M.; Jinnah, R. H.; and Ward, W. G.: Hip resurfacing with retention of existing hardware - case report. Bull NYU Hosp Jt Dis, 69 Suppl 1: S98-102, 2011.
97. Seyler, T. M.; Johnson, A. J.; Marker, D. R.; Mont, M. A.; and Bonutti, P. M.: Arthroscopic-assisted minimally invasive total knee arthroplasty. Arthroscopy, 27(2): 290-3, 2011.
98. Lang, J. E.; Mannava, S.; Floyd, A. J.; Goddard, M. S.; Smith, B. P.; Mofidi, A.; Seyler, T. M.; and Jinnah, R. H.: Robotic systems in orthopaedic surgery. J Bone Joint Surg Br, 93(10): 1296-9, 2011.
99. Mannava, S.; Plate, J. F.; Whitlock, P. W.; Callahan, M. F.; Seyler, T. M.; Koman, L. A.; Smith, T. L.; and Tuohy, C. J.: Evaluation of in vivo rotator cuff muscle function after acute and chronic detachment of the supraspinatus tendon: an experimental study in an animal model. J Bone Joint Surg Am, 93(18): 1702-11, 2011.
100. Bracey, D. N.; Seyler, T. M.; Shields, J. S.; Leng, X.; Jinnah, R. H.; and Lang, J. E.: A Comparison of Acetate and Digital Templating for Hip Resurfacing. Am J Orth, Under Review, 2012.
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101. Plate, J. F.; Seyler, T. M.; Halvorson, J. J.; Santago, A. C.; and Lang, J. E.: Non-Anatomic Closure of a Standard Parapatellar Knee Arthrotomy Leads to Patella Mal-Tracking and Decreased Range of Motion. Knee Surg Sports Traumatol Arthrosc, 2013 Jan 19. [Epub ahead of print]
102. Plate, J. F.; Seyler, T. M.; Stroh, A.; Akbar, M.; and Mont, M. A.: Risk of dislocation using large- vs. small-diameter femoral heads in total hip arthroplasty BMC Res Notes. Oct 5:553, 2012.
103. Schweppe, M. L.; Seyler, T. M.; Plate, J. F.; Swenson, R. D.; and Lang, J. E.: Does Surgical Approach in Total Hip Arthroplasty Affect Rehabilitation, Discharge Disposition, and Readmission Rate? Surg Technol Int, XXIII, 2013.
104. Seyler, T. M.; Whitlock, P. W.; Northam, C. N.; Smith, T. L.; Poehling, G. G.; Koman, L. A.; and Van Dyke, M. E.: The Effect of Cyclic Strain on the Tensile Properties of a Naturally-Derived, Decellularized Tendon Scaffold Seeded with Allogeneic Tenocytes and Associated mRNA Expression. J Surg Orthop Adv, 22,3:224-232, 2013.
105. Whitlock, P. W.; Seyler, T. M.; Parks, G. D.; Ornelles, D. A.; Smith, T. L.; Van Dyke, M. E.; and Poehling, G. G.: A Novel Process for Optimizing Musculoskeletal Allograft Tissue to Improve Safety, Ultrastructural Properties, and Cell Infiltration. J Bone Joint Surg Am, 94,16:1458-1467, 2012.
106. Mannava, S.; Plate, J. F.; Tuohy, C. J.; Seyler, T. M.; Whitlock, P. W.; Curl, W. W.; Smith, T. L.; and Saul, K. R.: The science of rotator cuff tears: translating animal models to clinical recommendations using simulation analysis. Knee Surg Sports Traumatol Arthrosc, 21,7:1610-1619, 2013.
107. Plate, J. F.; Mofidi, A.; Mannava, S.; Lorentzen, C. M.; Smith, B. P.; Seyler, T. M.; McTighe, T.; and Jinnah, R. H.: Unicompartmental Knee Arthroplasty: Past, Present, Future. Reconstructive Review, 2(2): 52-62, 2012.
108. Seyler, T. M.; Lorentzen, C. M.; and Jinnah, R. H. Femoro-acetabular Impingement: Prevalence and Importance in Patients Undergoing Hip Resurfacing. J Surg Orthop Adv, Under review, 2013.
109. Plate, J. F.; Pace, L. A.; Seyler, T. M.; Moreno, R. J.; Smith, T. L.; Tuohy, C. J.; and Mannava, S. Age-related Changes Affect Rat Rotator Cuff Muscle Function. J Shoulder Elbow Surg, 23,1:91-98, 2014.
110. Brown, P. J.; Sikes, C. V.; Mannava, S; Seyler, T. M.; Lang, J. E.; Stitzel, J. D. Biomechanical Comparison of A Single Large Diameter and Multiple Small Diameter Femoral Head Core Decompression Techniques. J Bone Joint Surg Am, Under Review, 2013.
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BOOK CHAPTERS
1. Frey, M. E.; O'Young, B. J.; Marulanda, G. A.; Mont, M. A.; and Seyler, T. M.: The Knee: Anatomy, Pathology, Diagnosis, Treatment, and Rehabilitation. In Physical Medicine and Rehabilitation, pp. 376-391. Edited by O'Young, B. J.; Young, M. A.; and Stiens, S. A., 376-391, Philadelphia, PA, Elsevier Mosby Saunders 2007.
2. Mont, M. A.; Marulanda, G. A.; Delanois, R. E.; Seyler, T. M.; and Friedman, A.: The Hip: Anatomy, Pathology, Diagnosis, Treatment, and Rehabilitation. In Physical Medicine and Rehabilitation, pp. 365-375. Edited by O'Young, B. J.; Young, M. A.; and Stiens, S. A., 365-375, Philadelphia, PA, Elsevier Mosby Saunders, 2007.
3. Seyler, T. M.; Marker, D. R.; and Mont, M. A.: Osteonecrosis. In Primer on the Rheumatic Diseases, pp. 565-572. Edited by Klippel, J. H.; Stone, J. H.; Crofford, L. J.; and White, P. H., 565-572, New York, NY, Springer Science + Business Media, LLC, 2007.
4. Seyler, T. M.; Mont, M. A.; Plate, J. F.; and Bonutti, P. M.: Valgus Approach to Total Knee Arthroplasty. In Navigation and MIS in Orthopaedic Surgery, pp. 240-247. Edited by Stiehl, J. B.; Konermann, W. H.; Haaker, R. G.; and DiGioia III, A. M., 240-247, Heidelberg, Germany, Springer Medizin Verlag, 2007.
5. Ulrich, S. D.; Mont, M. A.; Marker, D. R.; and Seyler, T. M.: Minimally Invasive Approach to Metal-on-Metal Total Hip Resurfacing Arthroplasty. In Minimally Invasive Surgery in Orthopedics, pp. 195-204. Edited by Scuderi, G. R., and Tria, A. J., 195-204, New York, NY, Springer Science + Business Media, LLC, 2010.
6. Lai, L. P.; Seyler, T. M.; and Mont, M. A.: Osteonecrosis of the Hip. In Total Hip Arthroplasty: The Essentials. Edited by Parvizi, J., and Klatt, B. A., Thorofare, NJ, SLACK Incorporated, 2012.
7. Marker, D. R.; Seyler, T. M.; Mont, M. A.; and McCarthy, E. F.: Osteonecrosis and Transient Osteoporosis: Diagnosis, Etiology, Treatment Options. In Surgery of the Hip. Edited by Berry, D. M., and Lieberman, J. R., Philadelphia, PA, Elsevier Mosby Saunders, 2012.
8. Whitlock, P. W.; Seyler, T. M.; Mannava, S.; and Poehling, G. G.: A Tissue-Engineered Approach to Tendon and Ligament Reconstruction. In Sports Injuries. Prevention, Diagnosis, Treatment and Rehabilitation. Edited by Doral, M. M.; Tandoğan, R. N.; Mann, G.; and Verdonk, R., Heidelberg, Germany, Springer Medizin Verlag, 2012
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MEETING PRESENTATIONS
1. Seyler, T. M.; Takemura, S.; Kang, Y. M.; Bram, R. J.; Kurtin, P. J.; Trousdale, R. T.; Goronzy, J. J.; and Weyand, C. M.: Biological Functions of BLyS and APRIL in Rheumatoid Synovitis. Arthritis & Rheumatism, 2003, Volume 48, Number 9, 67th Annual Scientific Meeting American College of Rheumatology Orlando, FL, 2003.
2. Seyler, T. M.; Park, Y. W.; Takemura, S.; Kang, Y. M.; Bram, R. J.; Goronzy, J. J.; and Weyand, C. M.: BLyS and APRIL in Rheumatoid Synovitis. Clinical and Investigative Medicine, 2004, Volume 27, Number 4, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 2004.
3. Ragland, P. S.; Mont, M. A.; Marulanda, G. A.; Delanois, R. E.; and Seyler, T. M.: Use of Metal-on-metal Resurfacing Arthroplasty For Avascular Necrosis of the Hip. Podium Presentation, 13th Annual Meeting of Association Research Circulation Osseous (ARCO), Imperial College, London, United Kingdom, 2005.
4. Mont, M. A.; Ragland, P. S.; Marulanda, G. A.; Delanois, R. E.; Flowers, N. B.; and Seyler, T. M.: Core Decompression For Secondary Osteonecrosis Of the Knee Using a Small-Diameter Drilling Technique. Podium Presentation, 13th Annual Meeting of Association Research Circulation Osseous (ARCO), Imperial College, London, United Kingdom, 2005.
5. Ragland, P. S.; Mont, M. A.; Marulanda, G. A.; Delanois, R. E.; Flowers, N. B.; and Seyler, T. M.: Use of a Proximally Hydroxyapatite-Coated Tapered Cementless Stem for Avascular Necrosis of the Hip. Podium Presentation, 13th Annual Meeting of Association Research Circulation Osseous (ARCO), Imperial College, London, United Kingdom, 2005.
6. Marulanda, G. A.; Seyler, T. M.; Delanois, R. E.; and Mont, M. A.: Percutaneous Drilling for the Treatment of Secondary Osteonecrosis of the Knee. Podium Presentation, 65th Annual Meeting Maryland Orthopaedic Society, Curtis National Hand Center, The Union Memorial Hospital, Baltimore, MD, 2006.
7. Marker, D. R.; Mont, M. A.; Seyler, T. M.; Hungerford, D. S.; and Jones, L. C.: Total Knee Arthroplasty in High Activity Patients. Best Resident/Fellow Research Award Maryland Orthopaedic Association, 65th Annual Meeting Maryland Orthopaedic Society, Curtis National Hand Center, The Union Memorial Hospital, Baltimore, MD, 2006.
8. Bhave, A.; Mont, M. A.; Seyler, T. M.; Marker, D. R.; and Delanois, R. E.: Functional Problems and Treatment Solutions Following Total Hip Arthroplasty. Podium Presentation., 16th Annual Fall Meeting American Association of Hip and Knee Surgeons, Gaylord Texan Resort, Dallas, TX, 2006.
9. Bonutti, P. M.; Seyler, T. M.; McMahon, M.; Marker, D. R.; and Mont, M. A.: Unicompartmental Knee Arthroplasty: Implant Survival & Risk Factors at 11-year Follow-up. Poster Presentation, 25th Annual Meeting Mid-America Orthopaedic Association, Boca Raton Resort & Club, Boca Raton, FL, 2007.
10. Bonutti, P. M.; Seyler, T. M.; and Mont, M. A.: Comparison of a Midvastus and Subvastus Approach to Total Knee Arthroplasty. Poster Presentation, 25th Annual Meeting Mid-America Orthopaedic Association, Boca Raton Resort & Club, Boca Raton, FL, 2007.
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11. Ulrich, S. D.; Mont, M. A.; Marker, D. R.; and Seyler, T. M.: Incidence and Reasons for Wasting Components in the Operative Room. Can this Problem be Reduced? Podium Presentation, 66th Annual Meeting Maryland Orthopaedic Society, University of Maryland Medical Center, Baltimore, MD, 2007.
12. Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; and Mont, M. A.: The Effectiveness of Gram Stains From Multiple Mediums in Total Joint Arthroplasty. Podium Presentation, 66th Annual Meeting Maryland Orthopaedic Society, University of Maryland Medical Center, Baltimore, MD, 2007.
13. Wenz, W.; Akbar, M.; Annefeld, M.; Marker, D. R.; Mont, M. A.; and Seyler, T. M.: Glucosamine Sulfate Therapy Treatment for Articular Damage in Canine Model. Poster Presentation, 53rd Annual Meeting Orthopaedic Research Society, San Diego, CA, 2007.
14. Lancin, P.; Essner, A.; Seyler, T. M.; Mont, M. A.; Wang, A.; and Delanois, R. E.: Effect of Acetabular Cup Abduction Angle on UHMWPE Wear in Hip Simulator Testing. Poster Presentation, 53rd Annual Meeting Orthopaedic Research Society, San Diego, CA, 2007.
15. Lee, D. K.; Hwang, S.; Choi, K. B.; Lee, H. Y.; Yi, Y.; Mont, M. A.; Seyler, T. M.; Meschter, C.; Copeland, O.; and Lee, K. H.: Preclinical Studies of Retrovirally-transduced Human Chondrocytes Expressing TGF-Beta1 (TG-C). Podium Presentation, 53rd Annual Meeting Orthopaedic Research Society, San Diego, CA, 2007.
16. Strassmair, M.; Bosebeck, H.; Mont, M. A.; and Seyler, T. M.: The Use of a Type I Lyophilisate Collagen as Osteoinductive Factor in Pseudoarthroses of the Forearm. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
17. Bhave, A.; Delanois, R. E.; Mont, M. A.; Paley, D.; Plate, J. F.; and Seyler, T. M.: Peroneal Nerve Dysfunction after TKA: Combined Surgical and Physical Therapy Protocol. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
18. Bhave, A.; Mont, M. A.; Starr, R.; Delanois, R. E.; Marker, D. R.; and Seyler, T. M.: Gait and Strength Analysis for Successful Correction of Functional Problems Following THA. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
19. Bhave, A.; Seyler, T. M.; Starr, R.; Plate, J. F.; and Mont, M. A.: Analysis and Correction of Pathological Gait Patterns and Functional Problems after TKA. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
20. Mont, M. A.; Delanois, R. E.; Seyler, T. M.; Plate, J. F.; and Bhave, A.: Botulinum Toxin Type A Injections for the Management of Flexion Contractures Following TKA. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
21. Marulanda, G. A.; Mont, M. A.; Seyler, T. M.; and Delanois, R. E.: Percutaneous Perforations for the Treatment of Osteonecrosis of the Ankle. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
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22. Akbar, M.; Balean, G.; Seyler, T. M.; Mont, M. A.; Ludwig, K.; Gerner, H. J.; and Loew, M.: Do Paraplegic Patients Walk on Their Arms? A Matched Comparison of Paraplegic Patients and Controls. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
23. Marulanda, G. A.; Mont, M. A.; Seyler, T. M.; and Delanois, R. E.: Technique and Results of Percutaneous Perforations for Avascular Necrosis of the Shoulder. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
24. Bonutti, P. M.; Seyler, T. M.; McMahon, M.; Marker, D. R.; and Mont, M. A.: Unicompartmental Knee Arthroplasty: Implant Survival and Risk Factors at 11-year Follow-up. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
25. Kolisek, F. R.; Monesmith, E. A.; Jessup, N.; Davis, K.; Seyler, T. M.; and Mont, M. A.: Total Knee Arthroplasty Utilizing Cementless Keels and Cemented Tibial Trays: Ten Year Results. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
26. Clohisy, J. C.; Seyler, T. M.; Kolisek, F. R.; Delanois, R. E.; Plate, J. F.; and Mont, M. A.: Total Hip Arthroplasty in Patients Younger Than 25 Years of Age. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
27. Delanois, R. E.; Seyler, T. M.; Plate, J. F.; Marker, D. R.; and Mont, M. A.: Selection Criteria for Joint Aspirations as a Diagnositic Test for Infection After Joint Arthroplasty. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
28. Ketterl, R.; Seyler, T. M.; and Mont, M. A.: Treatment of Bone Defects Using a Collagen Based Osteoinductive Scaffold. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
29. Seyler, T. M.; Bhave, A.; Plate, J. F.; Marker, D. R.; Marulanda, G. A.; Delanois, R. E.; and Mont, M. A.: Functional Problems and Arthrofibrosis Following Total Knee Arthroplasty. Scientific Exhibit Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
30. Bonutti, P. M.; Seyler, T. M.; Marker, D. R.; Plate, J. F.; and Mont, M. A.: Inventing Orthopaedics: From Design to Working Product. Scientific Exhibit Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
31. Seyler, T. M.; Bonutti, P. M.; Kester, M. A.; and Mont, M. A.: The Use of an Alumina-on-alumina Bearing System in THA for Avascular Necrosis of the Hip. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
32. Plate, J. F.; Seyler, T. M.; Marker, D. R.; Delanois, R. E.; and Mont, M. A.: Are Gram Stains Necessary for the Diagnosis of Infected TKA? Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
33. Mont, M. A.; Seyler, T. M.; Marker, D. R.; Marulanda, G. A.; and Delanois, R. E.: Metal-on-metal Hip Resurfacing in Avascular Necrosis of the Femoral Head. Poster
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Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
34. Marker, D. R.; Plate, J. F.; Seyler, T. M.; Delanois, R. E.; and Mont, M. A.: Diagnosing Total Joint Arthroplasty Infections: A Standardized Protocol. Scientific Exhibit Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
35. Mont, M. A.; Perez, O. A.; Moskal, J. T.; Hozack, W. J.; Steinberg, M. E.; Stiehl, J. B.; Hungerford, D. S.; Marulanda, G. A.; and Seyler, T. M.: Hip Surgery Complications: Multicenter Study of Femoral Stem Fractures after Total Hip Arthroplasty. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
36. Marker, D. R.; Seyler, T. M.; Delanois, R. E.; Plate, J. F.; and Mont, M. A.: The Sensitivity of Gram Stains from Multiple Sites in Patients with Infected Total Hip Arthroplasty. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
37. Mont, M. A.; Bonutti, P. M.; Marker, D. R.; Plate, J. F.; Marulanda, G. A.; and Seyler, T. M.: Minimally Invasive Knee Arthroplasty: Early Complications and Difficulties. Scientific Exhibit Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
38. Mont, M. A.; Delanois, R. E.; Plate, J. F.; and Seyler, T. M.: Femoral Neck Fractures Following Metal-on-metal Total Hip Resurfacing. Podium Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
39. Mont, M. A.; Amstutz, H. C.; Boyd, H. S.; Schmalzried, T. P.; Vail, T. P.; Sparling, E. A.; Kennedy, W. R.; Seyler, T. M.; and Goldberg, V. M.: Modern Generation Metal-on-metal Total Hip Resurfacings: Results of a Prospective FDA-IDE Study. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
40. Mont, M. A.; Jones, L. C.; Marulanda, G. A.; Seyler, T. M.; and Hungerford, D. S.: Effect of Epoetin Alfa on Blood Transfusions During Total Knee and Hip Arthroplasty. Poster Presentation, 74th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2007.
41. Bonutti, P. M.; Mont, M. A.; Plate, J. F.; and Seyler, T. M.: Osteonecrosis Of The Knee After Laser And Radiofrequency-Assisted Arthroscopy: Treatment With Minimally Invasive Knee Arthroplasty. E-poster Presentation, 6th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Fortezza da Basso, Florence, Italy, 2007.
42. Bonutti, P. M.; Mont, M. A.; McMahon, M.; Ragland, P. S.; and Seyler, T. M.: Minimally Invasive Total Knee Arthroplasty: Complications and Pitfalls. Podium Presentation, 6th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Fortezza da Basso, Florence, Italy, 2007.
43. Seyler, T. M.; Bonutti, P. M.; Ragland, P. S.; and Mont, M. A.: Minimally Invasive Valgus Approach to Total Knee Arthroplasty: A comparative Study. E-poster Presentation, 6th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Fortezza da Basso, Florence, Italy, 2007.
44. Mont, M. A.; Bonutti, P. M.; Sharkey, P. F.; Hozack, W. J.; Zelicof, S. B.; Kolisek, F. R.; Purtill, J. J.; Rothman, R. H.; and Seyler, T. M.: Clinical Experience Using A
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Minimally Invasive Surgical Approach for TKA: Early Results of a Prospective Randomized Multicenter Study compared to a Standard Approach. E-poster Presentation, 6th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Fortezza da Basso, Florence, Italy, 2007.
45. Mont, M. A.; Seyler, T. M.; and Ragland, P. S.: Functional Limits in Patients with Successful Total Knee Arthroplasty. E-poster Presentation, 6th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Fortezza da Basso, Florence, Italy, 2007.
46. Mont, M. A.; Marker, D. R.; Ulrich, S. D.; and Seyler, T. M.: The Effect of High Impact Sports on Total Knee Arthroplasties. Podium Presentation, 17th Annual Fall Meeting American Association of Hip and Knee Surgeons, Gaylord Texan Resort, Dallas, TX, 2007.
47. Bonutti, P. M.; Ulrich, S. D.; Marker, D. R.; Mont, M. A.; and Seyler, T. M.: Unicompartmental Knee Arthroplasty: Implant Survival and Risk Factors for Implant Failure at 11-year Follow-Up. Poster Presentation., 17th Annual Fall Meeting American Association of Hip and Knee Surgeons, Gaylord Texan Resort, Dallas, TX, 2007.
48. Seyler, T. M.; Ward, W. G.; Sprinkle, D. I.; and Jinnah, R. H.: Does Computer-assisted Surgery Aid in Shortening the Learning Curve in Metal-on-metal Surface Replacement? Poster Presentation, 20th Annual Congress of the International Society for Technology in Arthroplasty, Paris, France, 2007.
49. Marker, D. R.; Seyler, T. M.; Shilt, J. S.; LaPorte, D. M.; Mont, M. A.; and Frassica, F. J.: Current Literature: An Educational Tool to Study Osteonecrosis for the Orthopaedic In-Training Examination. Podium Presentation, 14th Annual Meeting of Association Research Circulation Osseous (ARCO), Baltimore, MD, 2007.
50. Boes, L.; Bosebeck, H.; Ulrich, S. D.; Mont, M. A.; and Seyler, T. M.: Collagen-Based Osteoinductive Bone Void Filler for MIS-Treatment in Osteochondritis dissecans and Osteonecrosis. Podium Presentation, 14th Annual Meeting of Association Research Circulation Osseous (ARCO), Baltimore, MD, 2007.
51. Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; Srivastava, S.; and Mont, M. A.: Do Modern Techniques Improve Core Decompression Outcomes for Hip Osteonecrosis? Podium Presentation, 14th Annual Meeting of Association Research Circulation Osseous (ARCO), Baltimore, MD, 2007.
52. Ketterl, R.; Denzel, C.; Bosebeck, H.; Mont, M. A.; Seyler, T. M.; and Ulrich, S. D.: Treatment of Fractures and Nonunions Using Type-I Lyophilisate Collagen as an Osteoinductive Factor. Poster Presentation, 23rd Annual Meeting Orthopaedic Trauma Association, Boston, MA, 2007.
53. Lee, R. K.; Lancin, P.; Bhatt, M.; Essner, A.; Wang, A.; Mont, M. A.; and Seyler, T. M.: Effect of Lubricant Protein Concentration on the Wear of UHMWPE Tibial Inserts in Knee Simulation. Poster Presentation, 6th Combined Meeting of the Orthopaedic Research Societies, Honolulu, HI, 2007.
54. Seyler, T. M.; Jinnah, R. H.; Marker, D. R.; and Mont, M. A.: Accuracy Of Diagnosing Periprosthetic Infections By Both Cultures And Tissue Sampling. Podium Presentation, 25th Annual Meeting Southern Orthopaedic Association, The Homestead, Hot Spring, VA, 2008.
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55. Seyler, T. M.; Marker, D. R.; Jinnah, R. H.; and Mont, M. A.: Influence Of Athletic Activities On Metal-On-Metal Total Hip Resurfacing Arthroplasty Outcomes. Podium Presentation, 25th Annual Meeting Southern Orthopaedic Association, The Homestead, Hot Spring, VA, 2008.
56. Seyler, T. M.; Jinnah, R. H.; Lai, L. P.; Sprinkle, D. I.; and Ward, W. G.: Does Computer-Assisted Navigation Aid In Resident Education And Shorten The Learning Curve n Hip Resurfacing? Podium Presentation, 25th Annual Meeting Southern Orthopaedic Association, The Homestead, Hot Spring, VA, 2008.
57. Whitlock, P. W.; Seyler, T. M.; Smith, T. L.; Poehling, G. G.; and Koman, L. A.: Effect of Strain on a Novel Cell-Seeded Scaffold for Tendon and Ligament Reconstruction. Poster Presentation, 54th Annual Meeting Orthopaedic Research Society, San Francisco, CA, 2008.
58. Bonutti, P. M.; Seyler, T. M.; Marker, D. R.; Rudert, L.; Dethmers, D.; Ulrich, S. D.; and Mont, M. A.: Navigation-Assisted TKA versus Minimally Invasive TKA: Benefits and Drawbacks. Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
59. Kolisek, F. R.; Mont, M. A.; Jessup, N.; Marker, D. R.; Seyler, T. M.; and Barnes, C. L.: A Prospective Randomized Study of Posterior-Stabilized vs. Posterior Cruciate Ligament-Retaining TKA. Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
60. Marker, D. R.; Seyler, T. M.; Lee, M.; Ulrich, S. D.; Delanois, R. E.; Akbar, M.; and Mont, M. A.: Does Metal-on-Metal Resurfacing Provide Comparable Results to Standard THA? Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
61. Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; Martimbianco, A. L.; Delanois, R. E.; and Mont, M. A.: Influence of Athletic Activities on Metal-on-Metal Hip Resurfacing Arthroplasty Outcomes. Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
62. Marker, D. R.; Seyler, T. M.; Ulrich, S. D.; Martimbianco, A. L.; Srivastava, S.; and Mont, M. A.: High Impact Sports After THA: Is the Bearing Type an Independent Predictor of Activity Level? Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
63. Marker, D. R.; Seyler, T. M.; Delanois, R. E.; Ulrich, S. D.; Srivastava, S.; and Mont, M. A.: Treatment of Early-Stage Osteonecrosis of the Femoral Head. Scientific Exhibit Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
64. Mont, M. A.; Seyler, T. M.; Kolisek, F. R.; Ulrich, S. D.; Bonutti, P. M.; and Marker, D. R.: Is Functional Improvement After TKA Related to Increased Sports Activity in Osteoarthritis Patients? Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
65. Mont, M. A.; Seyler, T. M.; Vogelstein, B.; Diaz, L. A., Jr.; Conway, J. D.; Kinzler, K. W.; Foss, C. A.; Ulrich, S. D.; and Pomper, M. G.: Imaging of Musculoskeletal Bacterial Infections by [124]FIAU-PET/CT. Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
66. Mont, M. A.; Ulrich, S. D.; Delanois, R. E.; Marker, D. R.; Akbar, M.; and Seyler, T. M.: Accuracy of Cultures and Tissue Sampling in Diagnosing Periprosthetic
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Infections? Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
67. Srivastava, S.; Mont, M. A.; Marker, D. R.; Ulrich, S. D.; Delanois, R. E.; and Seyler, T. M.: Clinical Efficacy of rhBMP-2 and rhBMP-7: Meta-Analysis of the Literature. Scientific Exhibit Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
68. Seyler, T. M.; Marker, D. R.; Ulrich, S. D.; Akbar, M.; and Mont, M. A.: Nonvascularized Bone Grafting for Avascular Necrosis of the Femoral Head. Paper Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
69. Seyler, T. M.; Smith, B. P.; Marker, D. R.; Ma, J.; Shen, J.; Smith, T. L.; Mont, M. A.; and Koman, L. A.: Botulinum Neurotoxin as a Therapeutic Modality in Orthopaedics: 20 Years of Experience. Scientific Exhibit Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
70. Srivastava, S.; Ulrich, S. D.; Marker, D. R.; Mont, M. A.; and Seyler, T. M.: Scientific Evidence for the Use of rhBMP-2 and rhBMP-7. Paper Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
71. Ulrich, S. D.; Bonutti, P. M.; Marker, D. R.; Dethmers, D. A.; Reinhold, J. H.; Robinson, Y.; Jones, L. C.; Seyler, T. M.; and Mont, M. A.: Computer-Assisted Surgery in Total Joint Arthroplasty: Complications, Pitfalls, and Solutions. Scientific Exhibit Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
72. Ulrich, S. D.; Childress III, L.; Marker, D. R.; Seyler, T. M.; and Mont, M. A.: Intra-Articular Injections of the Knee: A Prospective, Randomized Study to Evaluate Pain Intensity. Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
73. Ulrich, S. D.; Mont, M. A.; Seyler, T. M.; Marker, D. R.; Morrey, B. F.; and Bonutti, P. M.: Evaluation of a 30-Minute Protocol to Restore Range of Motion in Posttraumatic Elbow contractures. Podium Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
74. Ulrich, S. D.; Mont, M. A.; Seyler, T. M.; Marker, D. R.; Robinson, Y.; Delanois, R. E.; and Bonutti, P. M.: Stress Relaxation and Static Progressive Stretch in the Treatment of Shoulder Contractures. Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
75. Bonutti, P. M.; Marker, D. R.; Ulrich, S. D.; McMahon, M.; Rudert, L.; Seyler, T. M.; and Mont, M. A.: Evaluation of an All-Polyethylene Tibial Component Design in UKA. Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
76. Bonutti, P. M.; Mont, M. A.; Seyler, T. M.; Childress III, L.; Marker, D. R.; Delanois, R. E.; and Ulrich, S. D.: The Use of Stress Relaxation and Static Progressive Stretch in Postoperative Wrist Contractures. Poster Presentation, 75th Annual Meeting American Academy of Orthopaedic Surgeons, San Francisco, CA, 2008.
77. Akbar, M.; Bresch, B.; Seyler, T. M.; Bruckner, T.; Carstens, C.; and Abel, R. F.: Fractures in Myelomeningocele: A Trend and Risk Analysis. Podium Presentation,
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76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
78. McGrath, M. S.; Ulrich, S. D.; Bonutti, P. M.; Smith, J.; Seyler, T. M.; and Mont, M. A.: Evaluation of Static Progressive Stretch For the Treatment of Wrist Stiffness. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
79. Ulrich, S. D.; Bhave, A.; McGrath, M. S.; Seyler, T. M.; Marker, D. R.; Delanois, R. E.; and Mont, M. A.: Botulinum Toxin Type A Injections for the Management of Muscle Tightness Following THA. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
80. Akbar, M.; Bresch, B.; Seyler, T. M.; Wentz, W.; Bruckner, T.; Abel, R. F.; and Carstens, C.: Management of Orthopaedic Sequelae of Congenital Spinal Disorders. Scientific Exhibit Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
81. Marker, D. R.; LaPorte, D. M.; Seyler, T. M.; McGrath, M. S.; Ulrich, S. D.; and Mont, M. A.: Orthopaedic Journal Publications and Their Role in the Preparation for the OITE. Scientific Exhibit Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
82. Akbar, M.; Brunner, M.; Seyler, T. M.; Grieser, T.; Gerner, H. J.; and Loew, M.: Risk Factors for Rotator Cuff Tears in Wheelchair Users? A Case Control Study. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
83. Bonutti, P. M.; Ulrich, S. D.; Marker, D. R.; Seyler, T. M.; McGrath, M. S.; McMahon, M.; and Mont, M. A.: A Comparison of Subvastus and Midvastus Approaches in Total Knee Arthroplasty. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
84. Marker, D. R.; Ulrich, S. D.; McGrath, M. S.; Seyler, T. M.; Delanois, R. E.; and Mont, M. A.: Factors that Influence Weight Gain or Loss Following Total Hip Arthroplasty. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
85. Seyler, T. M.; Marker, D. R.; Boyd, H. S.; McGrath, M. S.; and Mont, M. A.: Pre-operative Evaluation to Determine Surgical Candidates for Metal-on-metal Resurfacing. Scientific Exhibit Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
86. Bonutti, P. M.; Seyler, T. M.; Ulrich, S. D.; Delanois, R. E.; Marker, D. R.; McGrath, M. S.; Capello, W. N.; D'Antonio, J. A.; and Mont, M. A.: Effect of Obesity on Clinical Success and Patient Satisfaction in Total Hip Arthroplasty. Poster Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
87. Marker, D. R.; Seyler, T. M.; Blackett, J. W.; McGrath, M. S.; and Mont, M. A.: Patient Expectations Regarding Activity Level Following Total Hip Resurfacing Arthroplasty. Poster Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
88. Marker, D. R.; Seyler, T. M.; Bhave, A.; McGrath, M. S.; Ulrich, S. D.; and Mont, M. A.: Does Commitment to Rehabilitation Influence Clinical Outcome of Total Hip
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Resurfacing Arthroplasty? Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
89. McGrath, M. S.; Bonutti, P. M.; McKenzie, S. A.; Ulrich, S. D.; Seyler, T. M.; and Mont, M. A.: Static Progressive Stretch for the Treatment of Knee Stiffness Following Total Knee Arthroplasty. Podium Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
90. Whitlock, P. W.; Van Dyke, M. W.; Poehling, G. G.; Smith, T. L.; Marker, D. R.; Koman, L. A.; and Seyler, T. M.: Functional Tissue Engineering of Tendon and Ligaments. Scientific Exhibit Presentation, 76th Annual Meeting American Academy of Orthopaedic Surgeons, Las Vegas, NV, 2009.
91. Seyler, T. M.; Maguire, C. M.; Jinnah, R. H.; and Reichard, S.: Metal-on-metal Hip Resurfacing in Young Patients: The Role of Femoroacetabular Impingement. Poster Presentation, 26th Annual Meeting Southern Orthopaedic Association, Amelia Island Plantation, Amelia Island, FL, 2009.
92. Maguire, C. M.; Seyler, T. M.; and Jinnah, R. H.: Metal-on-metal Hip Resurfacing in Young Patients: The Role of Femoroacetabular Impingement. Podium Presentation, 22nd Annual Congress of the International Society for Technology in Arthroplasty, Big Island, HI, 2009.
93. Shields, J. S.; Seyler, T. M.; Maguire, C. M.; and Jinnah, R. H.: Computer-Assisted Navigation in Hip Resurfacing: Learning Curve in the Hands of an Experienced Hip Surgeon. Podium Presentation, 22nd Annual Congress of the International Society for Technology in Arthroplasty, Big Island, HI, 2009.
94. Smith, T. L.; Seyler, T. M.; Van Dyke, M. W.; Poehling, G. G.; and Whitlock, P. W.: Development, Characterization and Cell Seeding of a Naturally-Derived Scaffold for Anterior Cruciate Ligament Reconstruction. Poster Presentation, Annual Meeting Biomedical Engineering Society, Pittsburgh, PA, 2009.
95. Smith, T. L.; Whitlock, P. W.; Koman, L. A.; Van Dyke, M. W.; and Seyler, T. M.: Effect of Strain on a Novel Cell-Seeded Scaffold for Tendon and Ligament Reconstruction. Podium Presentation, Annual Meeting Biomedical Engineering Society, Pittsburgh, PA, 2009.
96. Mannava, S.; Callahan, M. F.; Koman, L. A.; Plate, J. F.; Seyler, T. M.; Smith, T. L.; and Tuohy, C. J.: Novel Use of Electromyography (EMG) to Evaluate In Vivo Rotator Cuff Muscle Function After Acute and Chronic Detachment of the Supraspinatus Tendon: An Experimental Study in an Animal Model Poster Presentation, 27th Annual Meeting Southern Orthopaedic Association, El Conquistador, Fajardo, Puerto Rico, 2010.
97. Mannava, S.; Callahan, M. F.; Koman, L. A.; Plate, J. F.; Seyler, T. M.; Smith, T. L.; and Tuohy, C. J.: Evaluation of In Vivo Rotator Cuff Muscle Function After Acute and Chronic Detachment of the Supraspinatus Tendon: An Experimental Study in an Animal Model. Podium Presentation, 27th Annual Meeting Southern Orthopaedic Association, El Conquistador, Fajardo, Puerto Rico, 2010.
98. Seyler, T. M., and Jinnah, R. H.: Clinical Outcomes of Lateral UKA Using an All-Polyethylene Design. Podium Presentation, 27th Annual Meeting Southern Orthopaedic Association, El Conquistador, Fajardo, Puerto Rico, 2010.
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99. Seyler, T. M.; Jinnah, R. H.; and Plate, J. F.: The Effect of Bearing Type on Postoperative Activity Level After THA. Poster Presentation, 27th Annual Meeting Southern Orthopaedic Association, El Conquistador, Fajardo, Puerto Rico, 2010.
100. Seyler, T. M.; Bosebeck, H.; Buchner, H.; Vogt, S.; Hofmann, R.; Walter, G.; Hirschberger, W.; Stemberger, A.; and Kiokekli, M.: Preclinical and Clinical Experience of a Novel Calcium Sulfate/Carbonate Based Bone Substitute with Antibiotic Release. Poster Presentation, 56th Annual Meeting Orthopaedic Research Society, New Orleans, LA, 2010.
101. Marker, D. R.; Zywiel, M. G.; Stroh, A.; Strimbu, K.; McGrath, M. S.; Seyler, T. M.; and Mont, M. A.: The Use of Erythrocyte Sedimentation Rates and C-Reactive Protein Levels to Confirm TKA Infection. Poster Presentation, 77th Annual Meeting American Academy of Orthopaedic Surgeons, New Orleans, LA, 2010.
102. Akbar, M.; Doustdar, S.; Hemmer, S.; Seyler, T. M.; Furstenberg, C. H.; Lehner, B.; and Wiedenhofer, B.: Operative Treatment of Spinal Infections: Evaluation of Treatment Concepts from 1996-2008. Scientific Exhibit Presentation, 77th Annual Meeting American Academy of Orthopaedic Surgeons, New Orleans, LA, 2010.
103. Seyler, T. M.; Marker, D. R.; Bonutti, P. M.; Kolisek, F. R.; McGrath, M. S.; Zywiel, M. G.; Johnson, A. J.; and Mont, M. A.: Athletic Activity Following Total Hip Arthroplasty: Assessment and Outcomes. Scientific Exhibit Presentation, 77th Annual Meeting American Academy of Orthopaedic Surgeons, New Orleans, LA, 2010.
104. Hemmer, S.; Furstenberg, C. H.; Seyler, T. M.; Putz, C.; Ewerbeck, V.; Lehner, B.; Wiedenhofer, B.; and Akbar, M.: Primary and Metastatic Spinal Tumors: Evidence-based Treatment Strategies. Scientific Exhibit Presentation, 77th Annual Meeting American Academy of Orthopaedic Surgeons, New Orleans, LA, 2010.
105. Akbar, M.; Zastrow, R.; Bruckner, T.; and Seyler, T. M.: Upper Extremity Sequelae in Spinal Cord Injuries: An Epidemiological Survey Study of 670 Patients. Podium Presentation, 77th Annual Meeting American Academy of Orthopaedic Surgeons, New Orleans, LA, 2010.
106. Seyler, T. M.; Whitlock, P. W.; Smith, T. L.; Van Dyke, M. W.; and Poehling, G. G.: A Tissue-Engineered Scaffold Derived from Human Allograft Tissue for Use in ACL Reconstruction. Podium Presentation, OREF/ORS Atlantic Coast Region Resident Research Symposium, Durham, NC, 2010.
107. Mannava, S.; Saul, K. R.; Plate, J. F.; Seyler, T. M.; Koman, L. A.; Smith, T. L.; and Tuohy, C. J.: Rotator Cuff Dysfunction After Acute and Chronic Tears: Translating Animal Studies to Clinical Recommendations Using Simulation Analysis. Podium Presentation, OREF/ORS Atlantic Coast Region Resident Research Symposium, Durham, NC, 2010.
108. Mannava, S.; Saul, K. R.; Plate, J. F.; Seyler, T. M.; Koman, L. A.; Tuohy, C. J.; and Smith, T. L.: Rotator Cuff Dysfunction After Acute and Chronic Tears: Translating Animal Studies to Clinical Recommendations Using Simulation Analysis. Gold Medal Winner of Basic Science Award, Division of Surgical Sciences Research Day, Wake Forest University, School of Medicine, Winston-Salem, NC, 2010.
109. Seyler, T. M., and Jinnah, R. H.: Outcomes of Lateral UKA Using an All-Polyethylene Design. Podium Presentation, 41st Annual Meeting Eastern Orthopaedic Association, The Ritz Carlton Naples, Naples, FL, 2010.
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110. Smith, T. L.; Whitlock, P. W.; Seyler, T. M.; Northam, C. N.; Van Dyke, M. W.; Poehling, G. G.; and Koman, L. A.: Effect of Strain on the Tensile Properties and mRNA Expression of a Tissue-Engineered Flexor Tendon. Podium Presentation, 2010 Annual Meeting Biomedical Engineering Society, Austin Convention Center, Austin, TX, 2010.
111. Mannava, S.; Tuohy, C. J.; Seyler, T. M.; Stitzel, J. D.; Hayon, S.; Whitlock, P. W.; Smith, T. L.; and Saul, K. R.: The Science of Rotator Cuff Repairs: Translating Basic Science into Clinical Recommendations. Scientific Exhibit Presentation, 78th Annual Meeting American Academy of Orthopaedic Surgeons, San Diego, CA, 2011.
112. Mannava, S.; Seyler, T. M.; Pace, L. A.; Whitlock, P. W.; Smith, B. P.; Koman, L. A.; Smith, T. L.; and Tuohy, C. J.: Age-Related Changes Affect Supraspinatus Muscle Function: An Experimental Study in an Animal Model. Poster Presentation, 57th Annual Meeting Orthopaedic Research Society, Long Beach, CA, 2011.
113. Whitlock, P. W.; Seyler, T. M.; Smith, T. L.; Parks, G. D.; Van Dyke, M. W.; and Poehling, G. G.: A Tissue-Engineered Scaffold Derived From Human Allograft Tissue for Use in Anterior Cruciate Ligament Reconstruction. Poster Presentation, 57th Annual Meeting Orthopaedic Research Society, Long Beach, CA, 2011.
114. Sikes, C. V.; Brown, P. J.; Danelson, K. A.; Seyler, T. M.; Mannava, S.; Jinnah, R. H.; Stitzel, J. D.; and Lang, J. E.: Biomechanical Analysis of Hip Core Decompression Techniques and Fracture Risk. Poster Presentation, 57th Annual Meeting Orthopaedic Research Society, Long Beach, CA, 2011.
115. Mannava, S.; Koman, L. A.; Seyler, T. M.; Plate, J. F.; Tuohy, C. J.; Saul, K. R.; and Smith, T. L.: Translating Animal Studies to Clinical Recommendations with Computational Analysis for the Study of Rotator Cuff Dysfunction After Acute and Chronic Tears. Silver Medal Winner Integrative Science Category, Post-doctoral Research Day, Wake Forest University, Graduate School of Arts and Sciences, Winston-Salem, NC, 2011.
116. Whitlock, P. W.; Seyler, T. M.; Parks, G. D.; Smith, T. L.; Van Dyke, M. W.; and Poehling, G. G.: Development and Characterization of a Tissue-Engineered Scaffold For Anterior Cruciate Ligament Reconstruction Derived From Human Achilles Tendon Allograft. Podium Presentation, 8th Biennial Congress International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine, Riocentro Convention Center, Rio de Janeiro, Brazil, 2011.
117. Schweppe, M. L.; Goddard, M. S.; Seyler, T. M.; Plate, J. F.; Mannava, S.; and Lang, J. E.: A clinical comparative study of the direct anterior with the posterior approach in primary THA. Podium Presentation, Annual Meeting North Carolina Orthopaedic Association, Kiawah Island Golf Resort, Kiawah Island, SC, 2011.
118. Plate, J. F.; Mannava, S.; Seyler, T. M.; Saul, K. R.; Pace, L. A.; Smith, T. L.; and Tuohy, C. J.: Age influences Healing and Function of Rotator Cuff Muscles. Podium Presentation, Annual Meeting North Carolina Orthopaedic Association, Kiawah Island Golf Resort, Kiawah Island, SC, 2011.
119. Seyler, T. M.; Pulley, B.; and Lang, J. E.: A Multivariant Retrospective Analysis of Preoperative Nutritional Parameters as Predictors of Outcomes in Joint Arthroplasty. Podium Presentation, 31st Annual Oscar Miller Day Symposium, The Blake Hotel, Charlotte, NC, 2011.
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120. Plate, J. F.; Stone, A. V.; Seyler, T. M.; Marker, D. R.; Akbar, M.; and Mont, M. A.: Are Cultures and Frozen Sections Reliable in Diagnosing Periprosthetic Infections? Podium Presentation, 31st Annual Oscar Miller Day Symposium, The Blake Hotel, Charlotte, NC, 2011.
121. Plate, J. F.; Stone, A. V.; Seyler, T. M.; Marker, D. R.; Akbar, M.; and Mont, M. A.: Accuracy of diagnosing periprosthetic infections by both cultures and frozen sections. Podium Presentation, 99th Annual Meeting The Clinical Orthopaedic Society, Francis Marion Hotel, Charleston, SC, 2011.
122. Plate, J. F.; Stone, A. V.; Seyler, T. M.; Akbar, M.; and Mont, M. A.: Comparison of Dislocation Rates in Patients with large Diameter Metal-on-Metal and Small Diameter Metal-on-Polyethylene Bearings in Total Hip Arthroplasty. Podium Presentation, 99th Annual Meeting The Clinical Orthopaedic Society, Francis Marion Hotel, Charleston, SC, 2011.
123. Plate, J. F.; Seyler, T. M.; Halvorson, J. J.; Santago, A. C.; and Lang, J. E.: Non-Anatomic Closure of a Standard Parapatellar Knee Arthrotomy Leads to Patella Mal-Tracking and Decreased Range of Motion. Podium Presentation, Annual Meeting North Carolina Orthopaedic Association, Pinehurst Resort, Village of Pinehurst, NC, 2012.
124. Plate, J. F.; Seyler, T. M.; Halvorson, J. J.; Santago, A. C.; and Lang, J. E.: Non-Anatomic Closure of a Standard Parapatellar Knee Arthrotomy Leads to Patella Mal-Tracking and Decreased Range of Motion. Podium Presentation, 100th Annual Meeting The Clinical Orthopaedic Society (COS), The Palmer House, Chicago, IL, 2012.
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ELECTRONIC MEDIA
1. Mont, M.A.; Delanois, R.E.; Seyler, T.M.; Plate, J.F. New Treatment Approaches to Osteonecrosis of the Femoral Head. In: Marsh, J.L, ed. Instructional Course Lectures, Video Supplement. Vol. 56. Rosemont, IL: American Academy of Orthopaedic Surgeons, 2007.
2. Mont, M.A.; Marulanda, G.A.; Seyler, T.M.; Plate, J.F.; Delanois, R.E. Core Decompression and Nonvascularized Bone Grafting for the Treatment of Early-Stage Osteonecrosis of the Femoral Head. In: Marsh, J.L., ed. Instructional Course Lectures, Video Supplement. Vol. 56. Rosemont, IL: American Academy of Orthopaedic Surgeons, 2007.
3. Bonutti, P.M.; Mont, M.A.; Seyler, T.M.; Plate, J.F. Minimally Invasive Total Knee Arthroplasty: The Mini-Subvastus Approach. Orthopaedic Theaters. Vol. 8. Rosemont, IL: American Academy of Orthopaedic Surgeons, 2008.
4. Bonutti, P.M.; Mont, M.A.; Seyler, T.M.; Plate, J.F. Minimally Invasive Total Knee Arthroplasty: The Mini-Midvastus Approach. Orthopaedic Theaters. Vol. 8. Rosemont, IL: American Academy of Orthopaedic Surgeons, 2008.
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RESEARCH GRANTS
1. Seyler, T. M.; Poehling, G. G.; Whitlock, P. W.; Smith, T. L.; and Van Dyke, M. W.: A tissue-engineered approach to tendon and ligament reconstruction using human Achilles tendon allograft-derived scaffolds. Sponsor: Musculoskeletal Transplant Foundation. Amount: Non-Transplantable Tissue Program Grant. Duration: 2007.
2. Seyler, T. M.; Poehling, G. G.; Van Dyke, M. W.; Whitlock, P. W.; and Smith, T. L.: Tissue Engineering of Tendons and Ligaments Using Adipose-Derived Stem Cells and a Novel Naturally-Derived Scaffold. Sponsor: Orthopaedic Research and Education Foundation. Amount: $19,230.00. Duration: 7/2008 - 7/2008.
3. Seyler, T. M.; Poehling, G. G.; Whitlock, P. W.; Smith, T. L.; and Van Dyke, M. W.: A tissue-engineered approach to tendon and ligament reconstruction using human Achilles tendon allograft-derived scaffolds. Sponsor: Community Blood Center, Community Tissue Services, Dayton, Ohio. Amount: Non-Transplantable Tissue Program Grant. Duration: 2008.
4. Seyler, T. M.; Whitlock, P. W.; Smith, T. L.; Van Dyke, M. W.; and Poehling, G. G.: ACL Reconstruction in a Rabbit Model using a Novel, Biocompatible Human Achilles Allograft Tissue-derived Ligament Scaffold. Sponsor: Arthroscopy Association of North America. Amount: $24,980.00. Duration: 4/2008 - 4/2010.
5. Tuohy, C. J.; Mannava, S.; Seyler, T. M.; Smith, T. L.; Whitlock, P. W.; and Van Dyke, M. W.: Optimization of bioreactor preconditioning protocols for the maturation of seeded, naturally-derived scaffold. Sponsor: Wake Forest University Intramural Research Grant. Amount: $20,000.00. Duration: 3/2010 - 2/2012.
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