Keywords: importance of size, scaling, collagen, ascorbate, hydroxyproline
• Reading = Ch. 40 in Campbell 6th edition
Objectives of the second half of the course
• Learn how animals, plants, and bacteria work.
• Understanding of relationship between organism function and physical principles
• Linkages between biochemistry/cell biology and whole organism function/ecology
In the second half will discuss the basic functional needs of
organisms• What is an animal –animal diversity circulation and gas exchange
• nutrition• control of internal environment• chemical signaling• reproduction• nervous systems• sensory and motor mechanisms
This section introduces overall themes we will come back to:
• 1) Organisms have similar functional needs
• 2) Organisms must obey physical laws
• 3) Understanding how an organism works involves consideration of biochemistry, cell biology, physiology, ecology and evolution
Example: scaling of skeletons
• Is it possible to have 12 foot tall humans?
• Have to consider scaling effects
Cross sectional area = πr2
Mass increases to the third power of the linear dimension
What happens if you double thelinear dimension of an animal?
tissue
skeleton
To avoid weaker skeletons on large animals, the skeleton size
increases disproportionately
This Not This
The relationship
between skeleton size
and body mass for a variety of mammals
Body weight
Ske
leto
n w
eigh
t
1:1 relationship
actual
mouse
rat
man
elephant
A mouse-sized elephant
would have a skeleton
around 5 times heavier than a
mouse
Body weight
Ske
leto
n w
eigh
t
1:1 relationship
actual
mouse
rat
man
elephant
3) To understand how the functional needs of organisms are met, we need to integrate
information about:• Biochemistry
• Cell biology
• Physiology
• Evolution and Ecology
Collagen
• most abundant protein of mammals
• skin, bone, tendon, cartilage, and teeth
• Great tensile strength
• 3 helical polypeptides nearly 1000 residues long
• repeated (...glycine-x-x-glycine-x-x…) amino acid sequence
• Often Glycine-proline-hydroxyproline
What happens when there is faulty collagen: Scurvy
• Jacques Cartier 1536 exploration of the Saint Lawrence River
• “Some did lose all their strength, and could not stand on their feet.. Others also had all their skins spotted with spots of blood of a purple colour then did it ascend up to their ankles, knees, thighs, shoulders, arms, and necks. Their mouths became stinking, their gums so rotten, that all the flesh did fall off, even to the roots of the teeth, which did also almost all fall out.”
Why did this happen?
• Primates and guinea pigs cannot synthesize ascorbate (Vitamin C)
• Ascorbate is vital for the enzymatic conversion of proline (pro) to hydroxyproline (hyp)
• In scurvy patients, collagen has an amino acid sequence of gly-X-pro rather than gly-X-hyp
Why does the improper amino acid sequence have deleterious
effects?
• Collagen of scurvy patients has a low melting temperature
• Melting temp = 24° C for gly-X-pro in scurvy patients compared with 58° C for gly-X-hyp in normal people
What about animals living at high temperatures?
• The pompeii worm lives on undersea volcanoes at temperatures reported to be as high as 80°C making it the hottest living metazoan (multicellular animal) known.
• This is well above the melting temperature for normal collagen -- efforts are underway to determine the biochemical basis for high temperature collagen in these worms.