Antibiotics – antibiotic resistance
1) is the cell sensitive?
- cell wall / membrane permeable?
- sensitive target present?
2) action at
- cell wall synthesis
- membrane functions
- replication
- transcription
- translation
3) resistance mechanism (nb. sometimes different mechanisms may be possible with the same antibiotic)
- block penetration of the cell (keep the cell free of antibiotic)
- degradation of the antibiotic (removal intra- or extracellularly)
- modification of the antibiotic (inactivate the agent)
- modification of the target (avoid the action)
- amplification of the target (titration of the agent : tolerance by excess target)
Dot-blot analysis of interferon-beta mRNA concentration.