www.nt.gov.au
Varietal screening trial to identify
banana varieties highly tolerant or
resistant to Fusarium oxysporum f.sp
cubense Tropical Race 4
Sharl Mintoff
DEPARTMENT OF PRIMARY INDUSTRY AND RESOURCES
www.nt.gov.au
Varietal trail screening for TR4 resistance
• Banana Plant Protection Program
– BA10020
• Assessing 27 Banana varieties
for resistance to TR4
• Trial taking place at Coastal
Plains Research Farm (NT)
• Planting occurred in June 2016
www.nt.gov.au
Varietal trail screening for TR4 resistance
• Currently 15 months into trial and
26 of 27 varieties now in first ratoon
• Trial includes 4 reference varieties
– Williams (Susceptible)
– GCTCV 218 (Tolerant)
– FHIA 01 (Resistant)
– FHIA 25 (Highly resistant)
• This talk mainly focuses on the
health of the mother plants
Variety GCTCV 105/6
Cav
end
ish
Williams GCTCV 215
DPM25 GCTCV 217
Dwarf Nathan GCTCV 218 (Formosana)
Dwarf Parfitt Offtype CJ19
SH-3362 Auto-tetraploid SH-3436
Pare
nts
/ H
ybri
ds
SH-3142 SH-3641
SH-3217 SH-3656
SH-3362 SH-3748
FHIA-01 (Goldfinger) FHIA-18
FHIAFHIA-02 FHIA-25
FHIA-03 FHIA-26
Senorita Pisang Gajih Merah
Oth
er
Dwarf Ducasse M. acuminata ssp. banksii
www.nt.gov.au
Varietal trail screening for TR4 resistance
• Currently 15 months into trial and
26 of 27 varieties now in first ratoon
• Trial includes 4 reference varieties
– Williams (Susceptible)
– GCTCV 218 (Tolerant)
– FHIA 01 (Resistant)
– FHIA 25 (Highly resistant)
• This talk mainly focuses on the
health of the mother plants
Variety GCTCV 105/6
Cav
end
ish
Williams GCTCV 215
DPM25 GCTCV 217
Dwarf Nathan GCTCV 218 (Formosana)
Dwarf Parfitt Offtype CJ19
SH-3362 Auto-tetraploid SH-3436
Pare
nts
/ H
ybri
ds
SH-3142 SH-3641
SH-3217 SH-3656
SH-3362 SH-3748
FHIA-01 (Goldfinger) FHIA-18
FHIAFHIA-02 FHIA-25
FHIA-03 FHIA-26
Senorita Pisang Gajih Merah
Oth
er
Dwarf Ducasse M. acuminata ssp. banksii
www.nt.gov.au
Symptoms of Foc
• External symptoms
– Splitting of the pseudostem
– Yellowing and eventual wilting
of older leaves
– Death of leaves
– Death of plant
• Internal symptoms
– Discoloration of xylem
tissue
www.nt.gov.au
Criteria assessed
Disease assessments
• Assessments done monthly,
once infections became
promininantly fortnightly
• Presence of external
symptoms
• Death dates of plant
• Presence of internal
symptoms upon death
Agronomic assessments
• Date of bunch emergence
• Plant height at bunch
emergence
• Date of harvest
• Bunch weights
• Presence of internal
symptoms
www.nt.gov.au
Susceptible varieties
• Extensive disease symptoms
• Williams
- Death before harvest
• DPM25
- Death before
bunching/harvest
• Dwarf Parfitt offtype
– Death before bunchingDPM 25 D. Parfitt
OfftypeWilliams
www.nt.gov.au
Susceptible varieties (continued)
FHIA 26 SH-3656Senorita
• Senorita
- Death before bunching
• FHIA 26
- Death before bunching
• SH-3656
– Death before harvest
• Few made it harvest
www.nt.gov.au
• Majority of plants make it to
harvest
• No internal or external symptoms
observed
Resistant varieties
– FHIA 01
– FHIA 02
– FHIA 03
– FHIA 18
– FHIA 25
– Pisang Gajih
Merah
– SH-3362
– SH-3217
– SH-3142
– SH-3748
– GCTCV 215
– CJ19
FHIA 01 FHIA 25PG Merah
CJ19 GCTCV 215SH-3217
www.nt.gov.au
• SH-3362 Auto-tetraploid
– No signs of bunching, cut down 12
months after planting
– No symptom observed
• M. acuminata ssp. banksii
– North QLD Native
– Approx. 40% died in the first 6
months (unlikely due to TR4)
– Remaining plants severely
stressed
Problematic varieties
www.nt.gov.au
Susceptibility to Foc TR4
0
20
40
60
80
100
% inte
rnal sym
pto
ms
Variety
0
20
40
60
80
100
Will
iam
s
DP
M25
Dw
arf
Nath
an
Dw
arf
Parf
itt…
GC
TC
V 1
05
/6
GC
TC
V 2
15
GC
TC
V 2
17
GC
TC
V 2
18
…
CJ19
FH
IA-0
1…
FH
IA-0
2
FH
IA-0
3
FH
IA-1
8
FH
IA-2
5
FH
IA-2
6
SH
-343
6
SH
-364
1
SH
-365
6
SH
-374
8
SH
-336
2 A
uto
-…
SH
-314
2
SH
-321
7
SH
-336
2
Sen
orita
Dw
arf
Du
casse
Pis
ang
Ga
jih…
M.
acum
ina
ta…
Pe
rce
nt d
ea
th
Variety
Death (TR4) Death (other)
www.nt.gov.au
0
10
20
30
40
50
60
70
80
90
100
Pe
rce
nt
pla
nt
su
rviv
al
to h
arv
es
t
Variety
Harvested Remaining
Survival of plants
www.nt.gov.au
0
10
20
30
40
50
60
70
80
90
100
Pe
rce
nt
of
sym
pto
m p
res
en
ce
/ a
bs
en
ce
Variety
Present Absent
Survival of plants (internal symptoms at harvest)
www.nt.gov.au
Weeks to bunch emergence
0
10
20
30
40
50
60W
eeks p
ost
pla
nti
ng
Variety
www.nt.gov.au
Plant height at bunch emergence
0
50
100
150
200
250
300
350
400H
eig
ht (c
m)
Variety
www.nt.gov.au
Weeks to harvest
0
10
20
30
40
50
60W
eeks p
ost pla
nting
Variety
www.nt.gov.au
Bunch weight
0
5
10
15
20
25
30
35
40
Weig
ht (K
g)
Variety
www.nt.gov.au
Conclusions
Assessments of 1st ratoon plants is ongoing
– Determine if resistance seen in Mother plants also apparent in ratoon
plants
Several varieties have shown promising resistance to TR4
– Agronomic characteristics may not be ideal in their current state
– Resistance vs. acceptable market quality (need both)
– Promising candidates have been selected for Mutagenesis in attempt
agronomic qualities of resistant plants in BA14014
www.nt.gov.au
Acknowledgements
Vu Tuan Nguyen (DPIR)
Chris Kelly (DPIR)
Lucy Tran-Nguyen (DPIR)
Sam Cullen (DPIR)
Cassie McMaster
Gavin Hunter
Sharon Hamill (DAF)
Stewart Lindsay (DAF)
Jeff Daniells (DAF)
Tony Pattison(DAF)
www.nt.gov.au
Thank you