CURRICULUM VITAE OF WEI GUO
TITLE: ASSISTANT PROFESSOR Address: Department of Animal Science University of Wyoming 1000 E. University Ave. (Dept. 3684) Laramie, WY 82071 Office: (307) 766-3429; Mobile: 608-239-4375 Email: [email protected] or [email protected] PERSONAL STATEMENT I have tenure-track appointment as Assistant Professor in the Department of Animal Science with 67.5% research, 27.5% teaching, and 5% service. A major focus of Guo lab (Muscle Biology Laboratory, MBL) is to understand the molecular basis and cell signaling pathways underlying cardiomyopathies, heart failure and skeletal muscle myopathy. Another major focus of MBL is to study the life course impact of fetal programming on cardiac and skeletal muscle development and function. Understanding of these mechanisms may help to develop novel therapeutic strategies for treatment of cardiac and skeletal muscle diseases, and also help to improve meat quality and quantity in livestock. My three ongoing projects are to 1) decipher the molecular mechanisms of RNA binding motif 20 (RBM20)-mediated myocardial stiffness in heart failure with preserved ejection fraction (HFpEF); 2) understand the molecular mechanisms regulating skeletal muscle function and metabolism; and 3) determine the impact of maternal obesity on striated muscle function of offspring during life course. My teaching philosophy is to provide a classroom environment where students are encouraged to express their own ideas and participate in their education experience. Although I stress individual responsibility for learning in the class, I also provide explanation and guidance regarding the course materials and subject matter. I want students to enjoy their interaction in my classroom and to be challenged by the intellectual discussion of course materials. EDUCATION 2009 Postdoctoral Fellow in Animal Science/Nutritional Science, University of Wisconsin-Madison, WI. 2004 Ph.D. in Molecular/Cellular Biology China Agricultural University, Beijing, China. 1999 B.S. in Biotechnology, China Agricultural University, Beijing, China. PROFESSIONAL EXPERIENCE 2013-Present Assistant professor, Animal Science, University of Wyoming, Laramie, WY 2013-present Faculty member of Molecular Cellular Life Science Program, University of Wyoming, Laramie,
WY 2013-present Faculty member of Biomedical Science Program, University of Wyoming, Laramie, WY 2013-present Faculty member of the Center for Fetal Programming, University of Wyoming, Laramie, WY 2013-present Faculty member of the Center for Cardiovascular Research and Alternative Medicine, University
of Wyoming, Laramie, WY 2013 Research Scientist, Cell and Regenerative Biology at School of Medicine and Public Health,
University of Wisconsin-Madison, WI 2011-2013 Research Scientist, Minitube of America, Inc., Verona, WI 2009-2011 Research Scientist, Animal Science, University of Wisconsin-Madison, Madison, WI 2005-2009 Research Associate, Animal Science and Nutritional Science, University of Wisconsin-Madison,
WI 2004-2005 Research Assistant Professor, National Institute of Communicable Disease Control and
Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
HONORS AND AWARDS 2018 Wyoming AES 2018 Outstanding Young Scientist Award 2017 Session chair in the NIH IDeA Western Regional Conference 2016 Wyoming INBRE Bioinformatics Program Award 2015 Outstanding Poster Presentation Award at American Heart Association in the China-USA
Cardiovascular Symposium, Orlando, FL 2014 Young Investigator Outstanding Presentation Award at the 9th Annual ACRE/CNAHA Cardiovascular
Research Symposium of American Heart Association, Chicago, IL. 2014 Session Chair at Xi’an International Cardiovascular Forum and the 19th Xijing- Mayo Clinic
Cardiovascular Symposium, Xi’an, China. 2011-2013 Honorary fellow, Animal Science, University of Wisconsin, Madison, WI 2012 Abstract and Oral presentation Award for Excellence at International Meeting of Myofilament
Protein Function, Madison, WI 2005 Second Place Award for Science and Technology Award from Beijing
Municipality, Beijing, China 2004 First Place Award for Scientific and Technological Achievements from Chinese Academy of
Agricultural Sciences (CAAS), Beijing, China 2003 Award for Technology Excellence at Annual meeting of State Key Lab, Beijing, China 1996-1999 First-class Scholarship at College of Biological Sciences of China Agriculture University, Beijing,
China PROFESSIONAL SERVICE 2018-oresent Ad hoc reviewer for Michigan Diabetes Research Center Regional Grant program 2018-present Ad-Hoc grant reviewer for National Natural Science Foundation of China 2018-present Grant reviewer for the American University of Beirut (AUB) grant review, Lebanon 2018-present Ad-Hoc reviewer for the Scientific Peer Advisory and Review Services division of the American
Institute of Biological Science 2016-present Ad-Hoc reviewer for the Mountain West Clinical and Translational Research Infrastructure Network
(CTR-IN) Pilot Grant 2016-present Abstract reviewer for the American Society of Cell Biology (ASCB) 2016-present Abstract reviewer for the American Heart Association (AHA) 2015-present Study section member for the American Heart Association (AHA) Funding Program 2010-present Journal reviewer for over 15 peer-reviewed journals including Gene, Proteomics-Clinical Applications,
BBA-Molecular Cell Research, BBA-Molecular Basis of Disease, Cellular Physiology and Biochemistry, Cardiovascular Toxicology, Nutrition, Metabolism and Cardiovascular Diseases, Applied Cell Biology, Cell Cycle, International Journal of Biological Science, Journal of Cellular Biochemistry, PlosOne, Physiological Genomics, Molecular and Cellular Biochemistry, Meat Science and FEBS Letters etc.
PRESENTATIONS 2018 Presentation in ECI, 2018 Myofilament meeting, Madison, WI, May 19th 2018 2018 Invited presentation in 2nd International Caparica Conference in Splicing, Lisbon, Portugal, July 18th
2018 2018 Invited presentation titled “A molecular bungee cord and its regulator in muscle tissues”, Department
of Animal Science, Northwest Agriculture and Forestry University, Yangling, China.
2017 Invited presentation titled “Titin, a giant protein in Cardiomyopathies”, National Center for Cardiovascular disease, Beijing, China.
2016 Invited presentation titled “Titin and Cardiomyopathies”, National Center for Cardiovascular disease, Beijing, China.
2015 Invited presentation titled “Protein isoform transition in cardiovascular disease, fetal programming and meat quality”, China Agricultural University, Beijing, China.
2015 Presentation at Early Career Scientific Symposium in the International Society for Heart Research (ISHR), Seattle, WA.
2014 Presentation at Early Career Investigator Symposium in the Myofilament Meeting, Madison, WI. 2014 Invited presentation in the 15th Tokyo RNA Club at Noncoding RNA Regulation at RIKEN in Japan. 2014 Invited presentation at Xi’an International Cardiovascular Forum and The 19th Xijing-Mayo Clinic
Cardiovascular Symposium in Xi’an China. Title: “Effect of isoform transition of sarcomeric protein-Titin on cardiomyopathies”.
2014 Invited presentation at Department of cardiology at Xijing Hospital, Fourth Military Medical University, Xi’an, China. Title: “Gene Alternative Splicing and Heart Failure”.
2014 Invited Presentation from Biomedical Science Program for the Chinese delegation. Title: “Sarcomeric protein isoform and heart muscle disease”.
2013 Invited presentation titled “Alternative splicing and heart disease- titin as an example”, China Agricultural University, Beijing, China.
PROFESSIONAL MEMBERSHIPS 2018-present Member, American Society for Biochemistry and Molecular Biology (ASBMB) 2015-present Member, International Society for Heart Research (ISHR) 2013-present Member, International Society for Animal Genetics (ISAG) 2012-present Member, The American Society for Cell Biology (ASCB) 2010-present Member, American Heart Association (AHA) 2007-present Member, American Biophysical Society (ABS) 2004-present Member, Animal Gene Mapping Discussion Group (ANGENMAP) SERVICE ON CAMPUS 2014-present Member for Department Academic Affairs Committee 2014-present Member for Department Search Committee 2014-present Member for Graduate Admission Committee of MCLS Ph.D. Program 2014-present Member for Graduate Committee (for ten graduate students) 2014-present Chair for Graduate Committee (for three graduate students) TEACHING 2014-present ANSC4050/5050, Animal Growth and Development, 3 credits 2014-present ANSC4061/5061, Cell Signaling, 3 credits FUNDING SUPORT ONGOING SUPPORT Multi-state Hatch Grant, USDA/NIFA, Guo (PI) 10/25/2015 – 10/24/2020 Title: Molecular Mechanisms Regulating Skeletal Muscle Function and Metabolism
AES Competitive Grant, Agriculture Experiment Station (AES) of University of Wyoming, USDA-NIFA, Guo (PI) 1/1/2016-12/31/2018 Title: Molecular Mechanisms Mediating the Effects of Maternal Obesity on Cardiac Function and Development in Fetuses and Offspring of Obese Mothers National Science Foundation (NSF), Guo (co-PI) 7/1/2016-6/30/2019 Title: Removal of time and fidelity constraints in MALDI-MS imaging of proteins in tissue UW-College Collaborative Grant, NIH-Wyoming INBRE; NIH P20 NIGMS 103432, Guo (co-PI) 9/10/2018 – 5/1/2020 Title: Restoring Cardiac Function in Failing RBM20-/- Rats Neogen GeneSeek Corp. Donation Funds for Research Support, Guo (PI) 2/28/2018-3/1/2020 Title: Optimal Genomic Selection in Cattle PENDING SUPPORT NIH R01HL141453 (score 33 for first submission), Guo (PI) 12/1/2018-11/30/2023 Title: Regulatory Mechanisms of RBM20 in TTN splicing during heart failure progression AHA 18IPA34170430, Guo (PI) 7/1/2018-6/30/2020 Title: The novel insight of RNA binding proteins in cardiac muscle function COMPLETED FUNDING SUPPORT INBRE3 Thematic Pilot Grant, NIH-Wyoming INBRE; NIH P20 NIGMS 103432, Guo (PI) 5/1/2015-4/30/2018 Title: Role of RBM20 in the regulation of cardiac gene splicing in heart failure Beginning Grant-In-Aid Grant, American Heart Association (AHA), Guo (PI) 1/1/2016-12/31/2017 Title: The role of posttranslational modification of RBM20 in regulating titin isoform transition UW-College Collaborative Grant, NIH-Wyoming INBRE; NIH P20 NIGMS 103432, Guo (PI) 9/30/2014 – 8/31/2015 Title: Cardiac function and molecular mechanisms of phenotype diffs in RBM20-/- Rats Faculty Grant-in-Aid, Research Office of University of Wyoming, Guo (PI) 7/1/2015-6/30/2016 Title: Effect of Maternal Obesity on Cardiac Function in Offspring of Obese Mothers PATENTS
Wei Guo, Marion Greaser, Michael Gotthardt, Norbert Hubner, (equally contribution). polynucleotides for diagnostic and prognostic of a cardiac disease. European Patent: application #: 10736605.6-1222/ Patent #: EP2446059 BOOK CHAPTER 1. Greaser, M.L. and Guo Wei. 2011. “Post-mortem Muscle Chemistry” In: “Handbook of Meat and Meat
Processing, Second Edition”, ed. YH Hui, CRC Press, Boca Raton, FL, pp70-78. 2. Greaser, M.L. and Guo Wei. 2014. “Proteins” In “Handbook of Muscle Foods Analysis, Second Edition”, ed,
Leo M.L.Nollet, Fidel Toldra, CRC Press, Boca Raton, FL, PP357-368 3. Guo Wei and Greaser, M.L. 2016. “Muscle Structure, Proteins and Meat Quality” in “New aspects of meat
quality-from genes to ethics”, ed. Peter Purslow, Elsevier, Philadelphia, PA, PP13-32 MANUSCRIPT UNDER REVIEW 1. Lin Z., Wei LM., Cai W., Wei G., Ford PF and Ying G. Simultaneous Quantification of Protein Expression
and Modifications by Top-down Targeted Proteomics: A Case of Sarcomeric Subproteome. Analytical Chemistry. 2018. Under revision (MS ID: ac-2017-03772u)
2. J. W. Schneider, S. Oommen, S.C. Goetsch, W. Guo, A.W. Coutts, S.L. Edgerton, K. Holst, Y. Kureshi, B. Edwards, B. Rasmussen, D.A. Webster, T. Melkamu, S.C. Fahrenkrug, H. Sun, L. Steinmetz, D. Frantz, D. Carlson, T. J. Nelson. Pathogenic RNA granule pathway to heart failure in RBM20 cardiomyopathy gene-edited pigs. Nature Medicine. 2018. Under revision (MS ID: NMED-A91898)
PUBLICATIONS 1. Qiurong Wang, Chaoqun Zhu., Mingming Sun., Rexiati Maimaiti., Stephen P. Ford, Peter W. Nathanielsz,
Jun Ren and Wei Guo. Maternal obesity impairs fetal cardiomyocytes contractile function in sheep. FASEB J. 2018. Accepted.
2. Mingming Sun, Ying Tan, Maimaiti Rexiati, Maolong Dong, and Wei Guo. Obesity is as a Common Soil for Premature Cardiac Aging and Heart Diseases - Role of Autophagy. Biochimica et biophysica acta Mol Basis Dis. 2018. DOI: 10.1016/j.bbadis.2018.09.004
3. Chen Z., Maimaiti R., Zhu C., Cai H., Stern A., Mozdziak P., Ge Y., Ford SP., Nathanielsz PW., and Guo W. Z-band and M-band titin splicing and regulation by RBM20 in striated muscles. J Cell Biochem., 2018. doi: 10.1002/jcb.27328. PMID: 30133019.
4. Wei Guo, Chaoqun Zhu, Zhiyong Yin, Qiurong Wang, Mingming Sun, Huojun Cao and Marion L. Greaser. 2018. Splicing factor Rbm20 regulates transcriptional network of titin associated and calcium handling genes in the heart. Int J Biol Sci; 14(4): 369-380.
5. Zhilong Chen, Jiangping Song, Liang Chen, Hanfang Cai, Chaoqun Zhu, Mingming Sun, Allysa Stern, Paul Mozdziak, Ying Ge, Warrie J. Means and Wei Guo. 2018. Characterization of TTN novex splicing variants across species and the role of RBM20 in novex specific exon splicing. Genes (Basel). 9(2): 86-100. PMID: 29438341
6. Maimaiti Rexiati, Mingming Sun and Wei Guo. 2018. Muscle-specific mis-splicing and heart disease exemplified by RBM20. Genes. 9(1): 18-31. PMID: 29304022
7. Zhu C, Guo W. 2017. Detection and quantification of the giant protein titin by SDS-agarose gel electrophoresis. MethodsX. 4:320-327.
8. Ren J, Yang L, Zhu L, Xu X, Ceylan AF, Guo W, Yang J, Zhang Y. 2017. Akt2 ablation prolongs life span and improves myocardial contractile function with adaptive cardiac remodeling: role of Sirt1-mediated autophagy regulation. Aging cell. 16(5):976-987. PMID: 28681509
9. Guo W, Sun M. 2018. RBM20, a potential target for treatment of cardiomyopathy via titin isoform switching. Biophys Rev. 10(1): 15-25. PMID: 28577155
10. Zhu C, Yin Z, Tan B, Guo W. 2017. Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner. Biochimica et biophysica acta Mol Basis Dis. 1863(9): 2363-2371. PMID: 28676430
11. Zhu C, Chen Z, Guo W. 2017. Pre-mRNA mis-splicing of sarcomeric genes in heart failure. Biochimica et biophysica acta. 1863(8):2056-2063. PMID: 27825848
12. Guo W, Sanchez Walk AR. 2017. Is RBM20 a Promising Target for HFpEF Therapy? Current Trends in Biomedical Engineering & Biosciences. 3(5):555625
13. Shuyi Wang, Wei Guo and Jun Ren. 2016. Stress signaling in paraquat-induced target organ toxicity. ROS. 1(2): 131-140.
14. Jipeng Ma, Linhe Lu, Wei Guo, Jun Ren and Jian Yang. 2016. Emerging Role for RBM20 and its Splicing Substrates in Cardiac Function and Heart Failure. Curr Pham Des. Pp. 4744-4751. PMID: 27396593.
15. Zachery R. Gregorich, Ying Peng, Jeremy Wolff, Wei Guo, Huseyin Guner, Justin Doop, Timothy A, Hacker, Ying Ge. 2015. Comprehensive assessment of chamber-specific and transmural heterogeneity in myofilament protein phosphorylation by top-down mass spectrometry, J Mol Cell Cardiol. 87: 102-112. PMID: 26268593
16. Dale C. Guenther, Grace H. Anderson, Saswata Karmakar, Brooke A. Anderson, Bradly A. Didion, Wei Guo, John P. Verstegen, and Patrick J. Hrdlicka. 2015. Invader probes: harnessing the energy of intercalation to facilitate recognition of chromosomal DNA for diagnostic applications. Chem. Sci. 6(8): 5006-5015. PMID: 26240741
17. Chaoqun Zhu, Zhiyong Yin, Jun Ren, Richard J. McCormick, Stephen P. Ford and Wei Guo. 2015. RBM20 is an essential factor for thyroid hormone-regulated titin isoform transition. J Mol Cell Biol. 7(1): 88-90. PMID: 25573899
18. Zhiyong Yin, Jun Ren and Wei Guo. 2015. Sarcomeric protein isoform transitions in cardiac muscle: A journey to heart failure. BBA-Molecular Basis of Disease. 1852(1): 47-52. PMID: 25446994
19. Wei Guo, Jonathan M. Pleitner, Kurt W. Saupe and Marion L. Greaser. 2013. Pathophysiological defects and transcriptional profiling in the Rbm20-/- rat model. PLOS ONE. 8(12): e84281. PMID: 24367651
20. Li S, Guo W, Dewey CN, Greaser ML. 2013. Rbm20 regulates titin alternative splicing as a splicing repressor. Nucleic Acids Res. 41(4): 2659-2672. PMID: 23307558 (Featured Article)
21. Wei Guo, Schafer S, Greaser ML, Radke MH, Liss M, Govindarajan Gotthardt M et al. 2012. RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing. Nat. Med. 18: 766-773. PMID: 22466703 (Article received an Editorial in Nat. Med. (18: 660-661) and Dis. Model. Mech. (5:287).)
22. S.J. Li, Wei Guo, B. Schmitt, M. L. Greaser. 2012. Comprehensive Analysis of Titin Protein Isoform and Alternative Splicing in Normal and Mutant Rats. J Cell Biochem. 113(4): 1265-1273. PMID: 22105831
23. Jin Zhang, Wei Guo, Liangcai Shen, Qiuyue Liu, Xuemei Deng, Xiaoxiang Hu and Ning Li. 2012. Development of a porcine cDNA microarray: Analysis of clenbuterol responding genes in pig (Sus scrofa) internal organs. Journal of Integrative Agriculture. 11(11): 1877-1883.
24. Qiuyue Liu, Jin Zhang, Wei Guo, Yiqiang Zhao, Xiaoxiang Hu and Ning Li. 2012. Identifying lipid metabolism genes in pig liver after clenbuterol administration. Frontiers in Bioscience.E4: 2605-2612. PMID: 22652664
25. Wei Guo, Sheila J. Bharmal, Karla Esbona, and Marion L. Greaser. 2010. Titin Diversity-Alternative Splicing Gone Wild. J Biomed Biotechnol. 2010. PMID: 20339475.
26. Yuanhai You, Xun Zeng, Wei Guo, Yan Yin, Maojun Zhang and Jianzhong Zhang. 2009. Development of a multiplex PCR-microarray method for detection of important enteropathogen. China Biotechnology. 29(12): 79-84.
27. Marion L. Greaser, Chad M. Warren, Karla Esbona, Wei Guo,Yingli Duan, Amanda M. Parrish, Paul R. Krzesinski, Holly S. Norman, Sandra Dunning, Daniel P. Fitzsimons, and Richard L. Moss. 2008. Mutation that Dramatically Alters Rat Titin Isoform Expression and Cardiomyocyte Passive Tension. J Mol Cell Cardiol. 44(6), 983-91. PMID: 18387630 (Article received an Editorial: J Mol Cell Cardiol. 44(6): 976-978.)
28. W. Guo, S.H. Wang, H.J. Cao, K. Xu, J Zhang, Z. L. Du, W. Lu, J.D. Feng, Li Ning and C.H. Wu. 2008. Microarray comparison of gene expression in adipose tissue between Chinese Xiang pig and Large White pig. Asian-Aust. J. Anim. Sci. 21(1): 11-18.
29. J Zhang, W. Guo, BL Fan, WJ Zhu, LB Zheng, QY Liu, XM Deng, XX Hu and N Li. 2008. Apoliprotein D up-regulated in pig adipose tissue with administration of clenbuterol. Animal Husbandry & Veterinary Medicine. 1: 3-7.
30. Jin Zhang, Qiang He, Qiu Y. Liu, Wei Guo, Xue M. Deng, Wei W. Zhang, Xiao X. Hu, Ning Li. 2007. Differential gene expression profile in pig adipose tissue treated with/without clenbuterol. BMC Genomics. 8:433, PMID: 18039366
31. Q Wang, H.M. Xu, W. Guo. 2005. C8orf4 gene expression profile in Human Colorectal Cancer with different differentiation grades. Acad. J. PLA. P.G. Med. Sch. 26(6): 456-458.
32. W. Guo, M.X. Chu, X.M. Deng, J.D. Feng, Ning Li and Changxin Wu; 2004. Association of a single codon deletion in bone morphogenetic protein 15 gene with prolificacy in Small Tail Han Sheep. Asian-Aust. J.
Anim. Sci. 17(11): 1491-1495. 33. X Zhao, N Li, W Guo, X Hu, Z Liu, G Gong, A Wang, J Feng and C Wu. 2004. Further Evidence for
Paternal Inheritance of Mitochondrial DNA in the Sheep (Ovis aries); Heredity. 93(4): 399-403. PMID: 15266295
34. Mingxing Chu, Jinhua Cheng and Wei Guo. 2001. Preliminary studies of microsatellite markers OarAE101 and BM1329 in five sheep breeds. Acta Genetica Sinica., 28(6): 510�517. PMID: 11431983.
ABSTRACT 1. Chaoqun Zhu, Sreejayan Nair, Jun Ren and Wei Guo. RBM20 Knockout Ameliorates Angiotensin II-
Induced Hypertension and Cardiac Hypertrophy. 2018 Basic Cardiovascular Sciences (BCVS) meeting, San Antonio, TX, July 30th –August 2nd 2018.
2. Wei Guo, Chaoqun Zhu, Vikram Chhatre and Qiurong Wang. Diverse splicing mode of a giant gene TTN and its splicing regulation. The 2nd –international Caparica Conference in Splicing, Caparica, Portugal, July 16th-19th 2018.
3. Yutong Jin, Mingming Sun, Chaoqun Zhu, Wei Guo and Ying Ge. 2018. Characterization of RNA Binding Motif 20 Phosphorylation by Middle-down Mass Spectrometry. 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego, CA. June 3th -7th 2018.
4. Chaoqun Zhu, Sreejayan Nair, Jun Ren and Wei Guo. Rbm20 Deficiency Prevents Hypertension and Heart Failure Progression by Regulating Titin Size in Smooth Muscle. 2018 Myofilament Meeting, Madison, WI, May 19th -23rd 2018. Poster# 32.
5. Mingming Sun, Yutong Jin, Chaoqun Zhu, Maimaiti Rexiati, Hanfang Cai, Zhilong Chen, Martin Liss, Michael Gotthardt, Ying Ge and Wei Guo. Identification of RBM20 Phosphorylation and Its Role in The Regulation of Posttranscriptional Process of Myofilament Gene Titin. 2018 Myofilament Meeting, Madison, WI, May 19th -23rd 2018. Poster# 36.
6. Wei Guo. Giant elastic protein titin and its splicing regulator RNA binding motif 20 in the onset and development of heart failure. 2018 Myofilament Meeting, Madison, WI, May 19th -23rd 2018. Poster# 55.
7. Hanfang Cai, Zhilong Chen, Chaoqun Zhu, Rexiati Maimaiti, Qiurong Wang, Vikram Chhatre and Wei Guo. Concerted Splicing Regulation of RBM20 with Splicing Factors in Titin Z-, I- and M-band. 2018 Myofilament Meeting, Madison, WI, May 19th -23rd 2018. Poster# 37.
8. Maimaiti Rexiati, Mingming Sun and Wei Guo. RBM20 Modulates Myofiber Maturation and Skeletal Muscle Regeneration. 2018 Myofilament Meeting, Madison, WI, May 19th -23rd 2018. Poster# 40.
9. Rexiati Maimaiti, Zhilong Chen, Chaoqun Zhu, Hanfang Cai, Allysa Stern, Paul Mozdziak, Ying Ge, Stephen P. Ford, Peter W. Nathanielsz and Wei Guo. 2018. Z-band and M-band titin splicing and regulation by RBM20 in striated muscles. The Experimental Biology meeting, San Diego, CA., April 21-25, 2018.
10. Mingming Sun, Yutong Jin, Chaoqun Zhu, Maimaiti Rexiati, Hanfang Cai, ZHilong Chen, Ying Ge and Wei Guo. 2018. Mass spectrum analysis of RBM20 phosphorylation and its role in titin splicing. Accepted. The Experimental Biology meeting, San Diego, CA., April 21-25, 2018.
11. Maimaiti Rexiati, Mingming Sun and Wei Guo. 2018. RBM20 deficiency postpones skeletal muscle regeneration after injury and promotes fibrotic tissues formation. Accepted. The Experimental Biology meeting, San Diego, CA., April 21-25, 2018.
12. Zhilong Chen and Wei Guo. 2018. Splicing variation of TTN novex isoforms across species and RBM20 does not regulate splicing of novex isoforms in cardiac muscle. Accepted. The Experimental Biology meeting, San Diego, CA., April 21-25, 2018.
13. Qiurong Wang, John F. Odhiambo, Chris Pankey, Adel Ghnenis,Peter W. Nathanielsz, Stephen P. Ford, Wei Guo. Molecular Basis of Maternal Obesity Induced Fetal Cardiac Contractile Dysfunction. The AHA scientific meeting, Anaheim, CA. November 11th -15th 2017.
14. Qiurong Wang1, John F. Odhiambo1, Peter W. Nathanielsz1, Stephen P. Ford1, Jun Ren2, Wei Guo1. RBM39 is Vital for Maternal Obesity-Induced Fetal Sheep Cardiac Contractile dysfunction by Regulating Myocardial Autophagy. The AHA scientific meeting, Anaheim, CA. November 11th -15th 2017.
15. Qiurong Wang1,2, Chaoqun Zhu1, John F. Odhiambo1,2, Guadalupe L Rodríguez-González3, Peter W. Nathanielsz1,2, Stephen P. Ford1,2, Jun Ren4 and Wei Guo1,2. Maternal Obesity Compromises Mitochondrial Bioenergetic Profile of Term Fetal Sheep Heart. 64th SRI annual Scientific meeting, Orlando, FL. March 15th -18th 2017. (Oral Presentation)
16. ZIQING LIN; Liming Wei; Wenxuan CAI; Wei Guo; Stephen P Ford; Gary M. Diffee; Ying Ge. A Robust Top-down LC/MS-Based Proteomics Platform for Simultaneous Quantification of Protein Expression and Modifications. 65th ASMS Conference, Indianapolis, IN. June 4th -8th 2017.
17. Qiurong Wang, Chaoqun Zhu, Mingming Sun, Nan Hu, Stephen P. Ford, Peter W. Nathanielsz, Jun Ren, and Wei Guo. Maternal Obesity Causes Contractile Dysfunction of Term Fetal Sheep Cardiomyocytes. The AHA scientific meeting, New Orleans, LA. November 12th -16th 2016.
18. Zhilong Chen, Chaoqun Zhu, Mingming Sun, and Wei Guo. Characterization of transcriptional and posttranscriptional processing of Rbm20. 11th Annual Cardiovascular Research Symposium at the AHA scientific meeting, New Orleans, LA. November 12th -16th 2016.
19. Chaoqun Zhu, Zhiyong Yin and Wei Guo. Insulin Signaling Regulates Titin Splicing through RBM20. 6th Biennial National IDeA symposium of Biomedical Research Excellence (NISBRE), Washington, DC, June 26th-28th 2016. (Received Student Travel Award)
20. Chaoqun Zhu, Zhiyong Yin and Wei Guo. RBM20 is required for Insulin-induced pre-mRNA splicing of titin. Myofilament meeting, Madison, Wisconsin, June 17th -22nd 2016, Abs#17
21. Chaoqun Zhu, Stephen P. Ford, Peter W. Nathaielsz, Jun Ren and Wei Guo. Maternal Obesity Increases the stiffer, shorter titin isoform in the maternal and fetal heart. 63rd Annual Scientific Meeting of Society for Reproductive Investigation, Montreal, Canada, March 15-19, 2016, Abs#508.
22. Qiurong Wang, Chaoqun Zhu, Nan Hu, Stephen P. Ford, Petr W. Nathanielsz, Jun Ren and Wei Guo. Impact of Maternal Obesity and Overnutrition on Fetal Cardiac Function in Sheep. 63rd Annual Scientific Meeting of Society for Reproductive Investigation, Montreal, Canada, March 15-19, 2016, Abs#476.
23. Zhiyong Yin, Chaoqun Zhu, Jun Ren and Wei Guo. S107 improves RBM20 deficiency-induced cardiac dysfunction. 35th International Society for Heart Research-North American Section Meeting, Seattle, Washington, June 7-10, 2015, Abs#058. (Selected as Oral Presentation)
24. Chaoqun Zhu, Zhiyong Yin and Wei Guo. 2015. Insulin and Angiotension II regulate titin alternative splicing via Rbm20. Experimental Biology Meeting 2015, March 28-Apr 1, Boston, MS, Poster presentation. Abstract: FASEB J. 29: 562.16, 2015.
25. Andrea Sanchez Walk, Denise De Loera, Luiza Bosch, Wei Guo, Jun Ren and Herbert G Chew. 2015. Assessment of Cardiac Function Using Pressure Volume Loops in Rbm20-/- Rats. Experimental Biology Meeting 2015, March 28-Apr 1, Boston, MS, Poster presentation. Abstract: FASEB J. 29: 1047.7, 2015.
26. Chaoqun Zhu, Zhiyong Yin, Cun Li, Peter W. Nathanielsz and Wei Guo. 2015. IUGR alters the ratio of titin isoforms in term male baboon left atrium: potential impact on life-time cardiac function. The 62th SRI Annual Scientific Meeting, San Francisco, California, March 25-28, 2015. Poster Presentation, Abs650475
27. Chaoqun Zhua, Zhiyong Yina, Jun Ren, Richard J. McCormick, Stephen P. Ford and Wei Guo. 2014. RBM20 is an essential factor for thyroid hormone-regulated titin isoform transition via the PI3K/Akt pathway. The 9th Annual ACRE/CNAHA Cardiovascular Research Symposium of American Heart Association. Abs11. (Selected as poster oral presentation and received Outstanding Presentation Award)
28. Wei Guo, Chaoqun Zhu, Huojun Cao and Marion Greaser. 2014. Molecular signaling mechanism of striated muscle function and growth based on global gene expression analysis in Rbm20-/- rats (Rattus norvegicus). 34th International Society for Animal Genetics Conference, Xi’an, China, July 27-Aug. 1st, 2014, Abs648 (P3083).
29. Zhiyong Yin, Chaoqun Zhu, Xihui Xu, Nan Hu, Jun Ren, Marion Greaser and Wei Guo. 2014. Low thyroid induces Early disruption of cardiac function in Rbm20 deficient rats. Myofilament Meeting, Madison, Wisconsin, June 7-10, 2014. Abstract and Oral presentation, 7-Pos
30. Chaoqun Zhu, Zhiyong Yin and Wei Guo. 2014. T3-regulated titin isoform transition is Rbm20 dependent. 5th Biennial NISBRE meeting. Washington D.C., June 16-18, 2014, 133-Pos (Highlighted Poster and Received Student Travel award)
31. Zhiyong Yin, Chaoqun Zhu, Xihui Xu, Jun Ren and Wei Guo. 2014. Role of Rbm20 in cardiac dysfunction induced by low thyroid function. 5th Biennial NISBRE meeting. Washington D.C., June 16-18, 2014, 135-Pos (Highlighted Poster and Received Young Investigator Travel award)
32. Gregorich, Z., Guo, W., Hacker, T., and Y. Ge. 2013. Probing the transmural molecular heterogeneity of the heart using high-resolution top-down mass spectrometry. 61st American Society for Mass Spectrometry (ASMS) Conference, Minneapolis, Minnesota, June 9th–13th, 2013, Abstract, #WP 522.
33. Jonathan Pleitner, Jai Patel, Jill Koch, Timothy Hacker, Wei Guo, Marion Greaser. 2012. Effect of hyperthyroidism on wild type and mutant rats with altered cardiac titin expression. Myofilament Meeting, Madison, Wisconsin, June 11-15, 2012, Abstract, #36-Pos.
34. Wei Guo, Shijun Li, Sebastian Schafer, Herbert Schulz, Timothy Hacter, Hurt Saupe, Norbert Hubner, Michael Gotthardt, Marion Greaser. 2012. RBM20 regulates titin splicing, and defects result in cardiomyopathy. Myofilament Meeting, Madison, Wisconsin, June 11-15, 2012, Abstract and Oral presentation, 37-Pos.
35. Jonathan Pleitner, Wei Guo, Benjamin Schmitt, Jai Patel, Timothy Hacker, and Marion Greaser. 2012. Hypothyroidism Effects on Wild Type and Mutant Rats with Altered Cardiac Titin Expression. Biophys. J. Supplement, Abstract, 2841-Pos.
36. Wei Guo, Marion L. Greaser, Shijun Li, Herbert Schulz, Timothy A. Hacker, Kurt W. Saupe, Norbert Hubner, Michael Gotthardt. 2011. A Novel Splicing Factor that Affects Titin Alternative Splicing. Biophys. J. Supplement, Abstract, 2677-Pos.
37. Marion L. Greaser, J.R. Patel, W. Guo, H.S. Norman, S.Dunning, D.P.Fitzsimons, and R.L.Moss. 2010. Mechanical studies with hearts expressing a giant titin. Myofilament Meeting, Oral presentation. P17
38. Wei Guo, Jonathan M. Pleitner, Kurt W. Saupe, Timothy A. Hacker, and Marion L. Greaser. 2010. Characterization of a mutant rat model with altered titin isoform expression. Biophys. J. Supplement, Abstract, 2853-Pos.
39. Wei Guo, Shijun Li, Karla Esbona and Marion L. Greaser. 2008. Gene expression changes caused by mutation altering titin isoform splicing in rat cardiac muscle and skeletal muscle. Biophys. J. Supplement, Abstract, 1434-Pos.
40. Wei Guo, PhD and Marion Greaser, PhD. 2007. Messenger RNA changes in a rat strain with altered cardiac titin isoform expression. Biophys. J. Supplement, Abstract, 2963-Pos.
TRAINEES Student name Degree Training period Graduate status Current posiiton Chaoqun Zhu MS Fall 2013-summer 2015 Graduated student Mingming Sun PhD student Fall 2015-present Graduate in 2019 student Rexiati Maimaiti PhD student Fall 2016-present Graduate in 2021 student Chaoqun Zhu PhD student Fall 2015-present Graduate in 2019 student Postdocs Degree Current position Zhiyong Yin (2014.9-2015.9) PhD Associate Professor, Department of Cardiovascular
Medicine, Fourth Military Medical University, Xi’an, China Qiurong Wang (2015.6-present) PhD Postdoc Visiting Scholars Degree Current position Guorong Ruan (2015.10-2016.10) PhD Professor, Department head, Department of Animal Science,
Fujian Vocational College of Agriculture, Fuzhou, Fujian, China
ZHilong Chen (2015.12-2017.7) PhD Assistant professor, Department of Animal Science,
Northwest A&F University, Yangling, Shan’xi, China Wenliang Zha (2016.12-2017.5) PhD Associate Professor, School of Clinical Medicine, Hubei
University of Science and Technology, Xianning, Hubei, China
Hanfang Cai (2017.1-2019.1) MS PhD student Xin Jin (2017.7-present) MS visiting scholar
Undergraduate students Karly Higgins (2015.6-2016.12) Andrea Walk (2015. 2-2016.7) Denise J. De Loera (2015.6-present)
