RT-PCR WT E-I WT E-E 0.4 mM E-I 0.4 mM E-E 0.6 mM E-I0.6 mM E-E
0.8 mM E-I0.8 mM E-E 1.0 mM E-I 1.0 mM E-E 1.2 mM E-I1.2 mM E-E
Genomic E-I Genomic E-E 500 bp 100 bp 1000 bp Experimental Design
Control Water Injected 0.8 mM 4.1B-a MO 1.2mM 4.1B-a MO Acridine
Orange
Slide 14
IHC CaP VaP Colocalization Colocalization at CaP & VaP
Pre-Synaptic Marker Synapsin 1,2 Post-Synaptic Marker Pan MAGUK p
< 0.05 n = 6 n = 14 10 m
Slide 15
Touch Response Spinal Cord RB CoPA RDIN? PMN
Slide 16
Touch Response Control Response Morpholino Injected Response 40
ms 70 ms
Slide 17
Touch Response p < 0.02 n = 11 Control Morpholino
Injected
Slide 18
4.1B-a Knock-down: Reduces Excitatory Synapse Number in Primary
Motor Neurons Reduces Velocity of Touch Evoked Tail Swing Increases
Latency for Response to Touch 4.1B-a is Required for Proper
Development of the Touch-Response Neuronal Circuit Conclusions
Slide 19
Rescue Experiments of 4.1B-a Overexpression Experiments of
4.1B-a Analyze: Synapse Number at CoPA Cells Spontaneous Tail
Coiling Free Swimming Behavior at 3 dpf 4.1B-b Knock-Down
Experiments Future Directions
Slide 20
Phil Washbourne Acknowledgments Daniel Gibson Spencer Larson
Dan Fowler Renee Arias Francesco Argenton Domenico Cieri Yuqing Kou
Raluca Chebac Neena Leggett Paola Pizzo
Slide 21
Special Thanks UO SPUR Peter ODay NICHD R25 Summer Research
Program (NIH-1R25HD070817) Javier Fierro Jr.