Enzymatic Synthesis of Valuable Compounds Using Microbial CellsStep 1: Isolation and selection of microorganisms

· Screening of target enzyme· Evaluation of enzyme activity· Enhancement of the activity

Step 2: Enzyme characterization and gene cloning

Natural environment

Purecultures

Functional analysis Structural analysis

· Analyses of enzyme’s function and structure· Enzyme engineering· Construction of recombinant microbe

Geneticrecombination

Isolation ofmicroorganisms

Enzyme purification

Genecloning

Step 3: Enzymatic production of valuable compoundsMicrobial cellsof wild strainor recombinant

Rawmaterials

Enzymatic conversion

Valuableproducts

· Enzymatic synthesis under mild conditions　　　　　without by-products· Environmentally friendly system

Over-expressing cells