Institute for Risk Assessment SciencesVeterinary Public Health
Biosecurity en AB resistentie
Len LipmanIRAS/VPHFaculteit diergeneeskunde4 oktober 2015
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
Waarom maken we ons druk over AB resistentie?
Eten van vlees veroorzaakt antibioticaresistentie!!!!(Door: Wans Horst)Resistentie tegen antibiotica kan verschrikkelijke gevolgen hebben voor de wereldwijde bevolking. Daarom moet de resistentie bij de wortel worden aangepakt en niet bij de oplossing, zoals de Wereldgezondheidsorganisatie wil.
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
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Institute for Risk Assessment SciencesPublic Health and Food Safety Division
Wat is een volksgezondheid risico ikv gebruik AB bij dieren?
1.Er ontstaan AB resistente bacterien in het dier en die komen
daarna op vlees/in millieu.
2.Er komen residuen van AB in het vlees
1 of 2, of beide of beide niet?
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
In de bestrijding van ABR moeten we?
1. Dus minder AB gebruiken!!!
2. En slachtdieren niet in contact laten komen met
AB resistente bacteriën.
1, 2, beide of beide niet?
En is er een Relatie met biosecurity?
Associations between biosecurity, herd characteristics, production parameters
and antimicrobial usage in pig production in four EU countries
Merel [email protected]
Unit for Veterinary Epidemiology, Faculty of Veterinary Medicine, Ghent University
MINAPIG consortium – www.minapig.eu21/04/23 8
Annette Backhans, Lucie Collineau, Svenja Lösken, Marie Sjölund, Catherine Belloc, Ulf Emanuelson, Elisabeth Grosse Beilage, Elisabeth Okholm Nielsen, Katharina D.C. Stärk and Jeroen Dewulf
Objective
21/04/23 9
Which associations can be found between:
Biosecurity level
Herd characteristics
Production parameters
Antimicrobial usage
Best practises for low antimicrobial usage level
with maximum pig health and production
Materials & Methods
21/04/23 10
227 pig herds, ≥ 70 sows, 500 finishersBelgium, France, Germany, SwedenCross-sectional Dec. 2012 – Dec. 2013
Questionnaire & interviewProduction parametersHerd characteristicsBiosecurity statusAntimicrobial usage
Multivariable modelsCausal path
Discussion
Multi-country comparison
Causality
Country specific habits/situations
21/04/23 11
Take home message
Room for improvement Biosecurity & AMU
Better biosecurity more piglets weaned
Better biosecurity lower AMU
Higher weaning age lower AMU
21/04/23 12
Tools to improve animal health & productivity
and to minimise antimicrobial usage
21/04/23 13
The MINAPIG consortium
MINAPIG consortium – www.minapig.eu
MRSA and ESBL/AmpC producing Enterobacteriaceae
in vermin on Dutch farmsName: Joeri Baak
Student number: 3580822
Supervisor: dr. S.A. Burt
Sept 2014 – Dec 2014
Introduction
– Aim: determine whether flies, wild birds, mice and rats may carry antibiotic resistant bacteria in the animal husbandry in the Netherlands.
– MRSA and ESBL/AmpC producing E.coli, Enterobacter spp and Klebsiella spp.
MRSA
– Van de Giessen et al. 2009 isolated MRSA from five out of 40 black rats(Rattus rattus), 3 negative brown rats (Rattus norvegicus)
– MRSA isolated from mice and urban rats, black and brown rats, in Canada and Belgium (Pletinckx et al. 2013, Himsworth et al. 2014)
– ESBL producing E.coli isolated from urban brown rats in Berlin and Hong kong (Guenther et al. 2012, Ho et al. 2013)
– Blaak et al. 2014 isolated ESBL producing E.coli from two out of 19 fly pools on two poultry farms
– Veldman et al. 2013 isolated ESBL producing E.coli from one out of 41 non-aquatic wild birds and from 64 out of 373 aquatic wild birds
MacConkey-cefotaxime
ESBL
Materials and methods
– Sample collection• Wild birds• Flies• Rodents at Tolakker Utrecht
– Isolation of MRSA• MH-6.5% NaCl broth• PHMB (75 mg/L aztreonam and 4 mg/L oxacillin)• MRSA 2 brilliance plate (Oxoid)
– Confirmation tests (MRSA)• Coagulase test• Catalase test
Materials and methods
– Isolation of ESBL/AmpC producing Enterobacteriaceae
• LB-CTX broth
• TBX-CTX and MacC-CTX plates
– Confirmation tests (ESBL/AmpC)• Indole test
• Oxidase test
Results: Presumptive MRSA and ESBL/AmpC producing Enterobacteriaceae
Discussion: MRSA
– MRSA was isolated from a black rat
– In line with results of Van de Giessen et al. 2009 From five out of 40 black rats MRSA was isolated
Results: ESBL/AmpC producing E.coli Enterobakter
– ESBL producing E. coli isolated from a brown rat
– AmpC producing Enterobacter spp. isolated from two fly pools
– AmpC producing Enterobacter spp. isolated from a brown rat
Conclusion research
–Vermin may carry MRSA and ESBL/AmpC producing E. coli and Enterobacter spp. in the Dutch animal husbandry
–Biosecurity!!!!!
–Small number of samples investigated
Institute for Risk Assessment SciencesPublic Health and Food Safety Division
Is er een Relatie met biosecurity?
Ja dus
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