Laser desorption supersonic jet spectroscopy of hydrated tyrosine
June 18th, 2013
Hikari OBA
Tokyo Tech, Japan
Cooperative effect of hydrogen bond
*R. Nathaniel Pribble, et al.J. Chem. Phys., 106, 2145 (1997).
Bz-(MeOH)1
Bz-(MeOH)2
Why tyrosine-water cluster ?
Phenylalanine X
Tyrosine XR
Dopa
different ! almost same
[1] L. C. Snoek, et al., CPL, 2000 321 49. [2] Y. Shimozono, et al., PCCP, 2013, 15, 5163. [3] S. Ishiuchi, et al., PCCP, 2011, 13, 7812
Why tyrosine-water cluster ?
Stronger H-bond
To Investigate cooperative effect of separate hydrogen bond
more enhanced ?
Dopa
weaker H-bond
Tyr XR-(H2O)1
Laser desorption supersonic jet
REMPI and IR dip spectroscopyREMPI IR dip
REMPI spectra
bands 1bands 2
[1] Y. Shimozono, et al., PCCP, 2013, 15, 5163
[1]
REMPI
A)
B)
Different structures!
IR dip spectra of Tyr-(H2O)1 cluster
Assignment of water binding site
free OHH-bonded OH
* T. Ebata, et al., International Journal of Mass Spectrometry and Ion Processes, 1996, 159, 111.
*
A)
B)
Tyr-(H2O)1
Phenol
Phenol-(H2O)1
*
Assignment of water binding site
bands 1bands 2 (phenolic OH bound)
Assignment of conformation of tyrosine-(H2O)1
Tyr XR-(H2O)1
Tyr XR
Tyr XR-(H2O)1
[1]
[1] Y. Shimozono, et al., PCCP, 2013, 15, 5163
phenolic OH bound
Phe X
Tyr XR
Dopa
Tyr XR-(H2O)1
Comparison of the effect on hydrogen bond in amino acid
stronger H-bond
weaker H-bond
This work !
?
Importance of direction of dipole moments
Dopa
Tyr XR-(H2O)1
Different direction !
Summary
REMPI and IR dip spectra of tyrosine-(H2O)1 were measured.
Direction of dipole moments is important to enhance the hydrogen bond strength in cooperative effect.
Acknowledgment
Merci beaucoup !
Ms. Yoko Shimozono
Dr. Shun-ichi Ishiuchi
Prof. Masaaki Fujii Chemical Resources Lab., Tokyo Tech.
Chemical Resources Lab., Tokyo Tech.
Chemical Resources Lab., Tokyo Tech.
Dr. Pierre Çarçabal Institut des Sciences Moléculaires d’Orsay, U. Paris-Sud.
IR-UV Hole Burning spectra
Dipole moment of amino acid site and OH site
REMPI spectra of Tyr, Tyr-(H2O)1, Dopa
Assignment of conformation
A)
B)
Amino acid bound Phenolic OH bound
IR spectra of 12 conformers of tyrosine [1]
Assignment of amino acid chain
[1] Y. Shimozono, et al., PCCP, 2013.
