EEAD
Eucarpia, Lleida, November2006
Marker-trait association for disease resistance in the
Spanish Barley Core Collection
EEAD
Eucarpia, Lleida, November2006
Background - 1
• Spanish Barley Core Collection: 159 inbredlines from landraces (148 6-row, 11 2-row), 16 old cultivars
• Accessions are landraces, mostly collected in the first half of 20th century, prior tointroduction of modern cultivars, many ofthem by H.V. Harlan
• Passport data available
EEAD
Eucarpia, Lleida, November2006
Background - 2
• Population structure detected (73 markers). Four populations:
– 2-row
– 6-row European-like
– 6-row Spanish
– 6-row Spanish
EEAD
Eucarpia, Lleida, November2006
Background - 3
• Collection phenotyped for over 30 agronomic, morphological and quality traits (poster), andfor disease resistance
• Association mapping, taking into accountpopulation structure: multiple regression ofmarkers on trait variables, with probabilitiesof ascription to each population (Q) forcedinto the model.
• Multiple testing: Bonferroni, FDR
EEAD
Eucarpia, Lleida, November2006
Population structure
Spanish 6-rowSpanish 6-rowSpanish 2-row andEuropean 6-row
6-row
EEAD
Eucarpia, Lleida, November2006
Whole sample
LD issues6-rowSpanishCentral Plains
6-rowSpanishSouth andMediterraneancoast
EEAD
Eucarpia, Lleida, November2006
0,00
0,02
0,04
0,06
0,08
0,10
0,12
0,14
0-10 cM >10 cM no ligados
225 genotiposPoblación 1Población 2Población 3Población 4
Whole sample
Population 2Population 3Population 4
Population 1
0-10 cM not linked>10 cM
r2
r2
Distancia genética (cM)
0.0
0.2
0.4
0.6
0.8
1.0
0 50 100 150 200Genetic distance
LD issues
EEAD
Eucarpia, Lleida, November2006
0
3
6
9
12
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 1 2 3 4 5 6 7 8 9 10 11 12 13
Ppd-
H2
Ppd-
H1
eam
6
dens
o
eam
10
Vrn-
H2
eam
8
Number of populations
Chromosomes - Bins1H 2H 3H 4H
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12
eam
5
Vrn-
H1
eam
7
5H 6H 7H
0
3
6
9
12N
Number of populations
Number of populations
Chromosomes - Bins
Chromosomes - Bins
Headingdate
EEAD
Eucarpia, Lleida, November2006
Barley Yellow Dwarf Virus
0
10
20
30
40
50
0 1 2 3 4 5 6 7 8 9
Score
Num
ber
of a
cces
sion
s
Grain weight per plant
Degree of attack
CoracleVixen
EEAD
Eucarpia, Lleida, November2006
Relative grain weight > 80%0
10
20
30
40
50
0 1 2 3 4 5 6 7 8 9
Score
Perc
enta
ge
Degree of attack
Grain weight per plant
CoracleVixen
Barley Yellow Dwarf Virus
EEAD
Eucarpia, Lleida, November2006
Netblotch (Drechslera teres)Scald (Rhynchosporium secalis)
0
30
60
90
120
resistant mod. resistant mod. susceptible susceptible
Score
Num
ber o
f acc
essi
ons
D. Teres reactionScald score
Camelot Alexis
EEAD
Eucarpia, Lleida, November2006
D. Teres reaction
0
30
60
90
120
resistant mod. resistant mod. susceptible susceptible
Score
Num
ber o
f acc
essi
ons
D. Teres reaction
Netblotch, Drechslera teres
Resistant and mod. resistant
EEAD
Eucarpia, Lleida, November2006
0
20
40
60
resistant mod. resistant mod. susceptible susceptible
Score
Num
ber o
f acc
essi
ons
Scald score
Scald, Rhynchosporium secalis
Resistant
EEAD
Eucarpia, Lleida, November2006
Leaf rust, Puccinia hordei
0
10
20
30
40
1 2 3 4 5 6 7 8 9
Score
Num
ber o
f acc
essi
ons
Rust score
EEAD
Eucarpia, Lleida, November2006
Leaf rust, Puccinia hordei
0
10
20
30
40
1 2 3 4 5 6 7 8 9
Score
Num
ber o
f acc
essi
ons
Rust score
Resistant, scores 0, 1
EEAD
Eucarpia, Lleida, November2006
Powdery mildew (Blumeria graminis)
0
30
60
90
120
150
0 1 2 3 4
Score
Num
ber o
f acc
essi
ons
Race 9 Race 19Race 30 Race 78Race 79 Race 117Race 126
Alexis
Powdery mildew (Blumeria graminis)
EEAD
Eucarpia, Lleida, November2006
0
30
60
90
120
150
0 1 2 3 4
ScoreNu
mbe
r of a
cces
sion
s
Race 9 Race 19Race 30 Race 78Race 79 Race 117Race 126
Alexis
Average mean score < 1.5
Powdery mildew (Blumeria graminis)
EEAD
Eucarpia, Lleida, November2006
Average mean score < 1.5 0
30
60
90
120
150
0 1 2 3 4
ScoreNu
mbe
r of a
cces
sion
s
Race 9 Race 19Race 30 Race 78Race 79 Race 117Race 126
Alexis
Score=0, Race 19
EEAD
Eucarpia, Lleida, November2006
BYDV Degree of attack
Grain weight per plant
HvBM5 n Mean Mean4850 93 6.17 a 25.9 b 1200 50 3.63 b 55.4 a 150 9 4.14 b 61.9 a 1900 10 3.29 b 48.1 ab Bmag013 149 20 54.3 ab 153 10 49.3 ab 155 38 52.9 ab 157 14 41.3 bc 159 14 44.0 abc 161 18 34.8 c 163 16 56.4 a 165 9 49.7 abc Bmag120 230 12 42.3 ab 232 9 73.0 a 234 20 47.8 ab 236 48 50.5 a 244 9 32.4 c 262 9 48.5 ab 264 21 40.5 bc R2 40.9 54.3
EEAD
Eucarpia, Lleida, November2006
Rhynchosporium secalis
HvALAAT n Mean197 10 1.90 c 198 14 2.01 c 199 29 2.48 bc 200 23 2.36 bc 202 74 2.81 ab 203 18 3.30 a R2 8.9
EEAD
Eucarpia, Lleida, November2006
Blumeria graminis, race 19 HvBKASI n Mean 198 24 3.51 a 199 113 3.35 a 200 33 2.72 b R2 10.0
EEAD
Eucarpia, Lleida, November2006
Blumeria graminis, average
Bmac156 n Mean 135 14 1.47 c 137 26 2.15 a 139 19 1.91 ab 141 19 1.76 bc 143 26 2.19 a R2 17.7
Correlationcoefficient withfield trial scoresin Spain: 0.50
EEAD
Eucarpia, Lleida, November2006
Powdery mildew(Blumeria graminis)
Pictures: Ralph Panstruga
EEAD
Eucarpia, Lleida, November2006
Next step
• Development of ad hoc biparental populations:
– Powdery mildew, RILs population through SSD, currently in F5-F6 (IER-BAZ)
– Scald, DH populations in development by G Schweizer (LdL Bavaria)
• Continue association mapping. Results encouraging. More markers, more individuals?
EEAD
Eucarpia, Lleida, November2006
Resources
Active website for Spanish barley core collection:
http://www.eead.csic.es/EEAD/barley
DIVA-GIS:
http://www.diva-is.rg
EEAD
Eucarpia, Lleida, November2006
D Kopahnke, A Habehuß, F OrdonInstitute of Epidemiology and Resistance Resources, IER-BAZ (Aschersleben, Germany)
G SchweizerInstitute for Crop Production and Plant Breeding, LdLBavaria (Germany)
A Casas, MP Gracia, JM Lasa, E Igartua, S Yahiaoui, A CuestaAula Dei Experimental Station, CSIC (Zaragoza, Spain)
JL Molina-Cano, M MoralejoCentre UdL-IRTA (Lleida, Spain)
F Ciudad, P CodesalInstitutoTecnológico Agrario (Valladolid, Spain)
EEAD
Eucarpia, Lleida, November2006