Microbiology Overview
KWWOA
April 09, 2014
Department for Environmental ProtectionEnergy & Environment Cabinet
Topics
Topics
o Proficiency Tests
o Chain of Custody
o Methods
o Membrane Filtration
Proficiency Tests
Proficiency Tests
o Drinking Water: WS StudySW sample for MPN
o Wastewater: DMR-QA WP Study
o KY Approved PT Providers List
Proficiency Tests
Proficiency Tests
Proficiency Tests
Proficiency Tests
Revised reports will not be accepted.
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Chain of Custody
Chain of Custody
• Client name & address
• Sampler name
• Sample location
• Collection date & time
• Sample type
(grab/composite)
• Matrix (DW/WW)
• Field sample ID
• Laboratory sample ID
• Container type, & size/volume
• Number of containers
• Preservative (ice, etc.)
• Field data (pH, TRC, etc.)
• Requested analysis
• Reason for rejection or
special remarks
• Signature, date & time for
each transaction
(relinquishing & receiving)
• Sample temperature
upon arrival at lab
BARF is NOT a COC
Methods
Microbiology Methods
Method Drinking Water Wastewater
Colilert E. coli
Colilert-18 E. coliFecal – ATP @ 44.5oC
Colisure
Coliscan
mFC NA-MUG Fecal
mEndo EC-MUG
mColiBlue-24 E. coli
Modified mTEC E. coli
17
Colilert-18 P/A
Colilert-18 pre-warming
18
Colilert-18 MPN
Membrane Filtration
Membrane Filtration
Dilutions:
Select appropriate volumes
Fecal Coliform or E. coli colonies: 20-60*
Total colonies: 200*Use range as specified in particular method.
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Membrane Filtration
Blanks:• Beginning of series
• End of series
• RECORD results of blanks
Membrane Filtration
Calculating Results:• Use dilution that is in range to
determine result.
• If multiple plates within range, average those plate counts.
• If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments.
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Membrane Filtration
Calculating Results:
• If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments.
• WW TNTC = 60,000
Contact Information
Tiffany Nolan
Kentucky Department for Environmental ProtectionDivision of Water
200 Fair Oaks Lane; 4th FloorFrankfort, KY 40601
Phone: (502) 564-3410
Email: [email protected]