Multiple Sclerosis (MS) is an autoimmune-mediated demyelination disease whose precise molecular mechanism are still unknown. Proteolipid protein (PLP) and Myelin Oligodendrocyte Glycoprotein (MOG) are suspected antigens in the pathogenesis of MS. As such, these myelin proteins may be used as antigens to induce Experimental Autoimmune Encephalomyelitis (EAE), an animal model of the central nervous system (CNS) demyelinating diseases including MS.
AnaSpec, EGT Group supports MS/EAE research by providing a suite of peptides, assay kits and antibodies that facilitate the advancement of this research. Every lot of MOG (35-55) (Cat# AS-60130) peptide is tested for EAE induction in mice. ELISA assays for quantifying IgG of MOG or PLP are the first in the industry. As well, antibodies against MOG, PLP and MBP are available. n
Multiple Sclerosis & EAE The MOG and PLP
Sensolyte® Assay kits were carefully developed to
quantify by ELISA auto-antibodies associated with
Multiple Sclerosis.
BenefitsP Convenient Format
> Pre-coated and pre-blocked 96-well strip plate
> Ready-to-use substrate solutions and other assay components
P Minimal Sample Size> Requires only 0.5-1 μl of serum or
cerebrospinal fluid
P High Sensitivity
> Detects as low as 100 pg of anti-PLP
(139-151 or 178-191) IgG 100 pg anti-MOG (1-125) IgG 100 pg anti-MOG (35-55) IgG
P Broad Dynamic Range
> Detection of 8 to 500 ng antibody/ml serum (depending on the kit and the colorimetric
developing time)
Results
0
0.5
1
1.5
2
2.5
3
3.5
4
0 5 10 15 20
Aver
age
EAE
Scor
e
Day after peptide administration
MOG (35-55) EAE Graph
Industry’s first provider of anti-MOG and anti-PLP kits
© S
ebas
tian
Kau
litzk
i - F
otol
ia.c
om
© S
ebas
tian
Kau
litzk
i - F
otol
ia.c
om
Description
Dynamic Range
Detection Limit
Main kit contains Assay time Number of Assays in
96-well plate
Reference
SensoLyte® anti-MOG & anti-PLP Assay KitsMOG (Myelin Oligodendrocyte Glycoportein) ELISA KitSensoLyte® Anti-Mouse/Rat MOG (35-55) AssayColorimetric
8-500 ng/ ml 100 pgMOG (35-55) plate (12 x 8 strips)Mouse Anti-MOG (35-55) IgG Standard
2-3 hours 96 assays AS-54465
SensoLyte® Anti-Human MOG (1-125) Assay Colorimetric
1.56-100 µg/ml (AS-55153-H)
8-500 µg/ml (AS-55153-R; AS-55153-M)
1 ng/mL
Human MOG (1-125) coated plate (12 x 8 strips)Rat Anti-Human MOG (1-125) IgG Standard or Mouse Anti-Human MOG (1-125) IgG Standard or Human Anti-Human MOG (1-125) IgG Standard
2-3 hours 96 assaysAS-55153-RAS-55153-MAS-55153-H
SensoLyte® Anti-Mouse MOG (1-125) Assay Colorimetric
8-500 ng/ ml 100 pgMouse MOG (1-125) coated plate (12 x 8 strips)Anti-Mouse MOG (1-125) IgG Standard
2-3 hours96 assays AS-55156
SensoLyte® Anti-Rat MOG (1-125) Assay Colorimetric
8-500 ng/ ml 100 pgRat MOG (1-125) coated plate (12 x 8 strips)Anti-Rat MOG (1-125) IgG Standard
2-3 hours 96 assays AS-55157
PLP (Proteolipid Protein) ELISA Kit
SensoLyte® Anti-PLP (139-151) Assay Colorimetric
8-500 ng/ml 100 pgPLP (139-151) coated plate (12 x 8 strips)Mouse anti-PLP (139-151) IgG Standard
2-3 hours 96 assays AS-55524
SensoLyte® Anti-PLP (178-191) Assay Colorimetric
8-500 ng/ml 100 pgPLP (178-191) coated plate (12 x 8 strips)Mouse anti-PLP (178-191) IgG Standard
2-3 hours 96 assays AS-55523
Description
PR
O-M
ULT
IPLE
-041
5-V3
For more information and related products, please contact your local sales
representative.
s e r v i c e @ a n a s p e c . c o m w w w. a n a s p e c . c o m
Multiple Sclerosis & EAE
Related ProductsMOG Recombinant Proteins
Recombinant mouse MOG (1-125)
100 μg AS-55150-100
500 μg AS-55150-500
1000 μg AS-55150-1000
Recombinant rat MOG (1-125)
100 μg AS-55152-100
500 μg AS-55152-500
1000 μg AS-55152-1000
Recombinant human MOG (1-125)
100 μg AS-55158-100
500 μg AS-55158-500
1000 μg AS-55158-1000
MOG Peptides Every lot of MOG (35-55) peptide is tested for EAE induction
MOG (35-55), mouse, rat 1 mg AS-60130-1
MEVGWYRSPFSRVVHLYRNGK 5 mg AS-60130-5
10 mg AS-60130-10