Natuzyme A Mul- Enzymes Product
About Bioproton
• Bioproton is an Australia-‐ based biotechnology
company, with specializa:on in Feed Enzymes
• Founded in 1984 in Finland, but in 1993 relocated to
Brisbane, Australia
• Currently exports feed enzyme formula:ons to over
+40countries
• Produc:on and R&D facili:es are in Brisbane
Bioproton Partners
• Australian Ins:tute for Bioengineering
Nanotechnology (AIBN), in the Univ. of Queensland
• AIBN
– World renowned research centre
– Over 360 researchers housed in a $75 million state-‐of-‐the-‐art
building complimented by an extensive suite of facili:es.
• Bioproton-‐ Recipient of Australian Government’s
Australian Research Council Grants
Bioproton Quality Assurance
Enzymes in Animal Feed
• Enzymes being added to animal feed since 1980s in response to
Economic, Health and Environmental concerns
• Total enzyme use in animal feed is ~USD600M with 10% pa growth
• Phytase is the most common enzyme used worldwide, accoun:ng for
50% of all sales. Other enzymes, mainly NSP Degrading Enzymes,
account for the rest.
Pressure on Growers Today
What are NSPs? • NSP-‐ Non starch polysaccharides
• Old name-‐ Crude fibers
• Cellulose, Hemicellulose, Pec:ns and Oligosaccharides
• Water-‐ soluble and water-‐ insoluble NSPs
• NSPs harm the bird indirectly by-‐
a) Increased viscosity of the digest, resul:ng in lower passage rate of nutrients.
b) Entrapment of Nutrient ( Cage-‐effect). Entrapping other nutrients
mainly starch, fat and protein
c) Influence on energy density (Dilu:on effect)
NSPs Viscosity increasing polysaccharides
Increased intestinal viscosity
Reduced passage rate of digesta
Reduced feed intake Increased growth of intestinal flora
Nutrient competition between host and microflora
Reduced nutrient intake Increased intestinal viscosity
Reduced nutrient availability
Deterioration of growth performance and feed conversion (FCR)
Increase Viscosity-‐
NSPs
b) Encapsula-on (Cage effect)-‐
Entrap other nutrients mainly starch, fat and protein
This is applied to the insoluble parts of the NSP which are present
in the various cell wall structures
NSPs
c) Influence Energy Density (Dilu-on effect)-‐
Poorly digestible
Dilute the metabolizable energy
Dilute the nutrient content of feed
NSP and Energy
• Feed energy content determines the price of feed.
• NSP content inversely propor:onal energy content, so high NSP content feed is cheaper.
Role of Phytase
Role of Phosphorus • A vital and expensive macronutrient
• Forma:on and maintenance of skeletal structure (bone growth and calcifica:on)
• Metabolic processes (energy release and forma:on of amino acids)
• 3rd most expensive nutrient in feed (aeer energy and protein/amino acids)
• Adequate dietary phosphorus is required for rapid and efficient growth
• Availability of phosphorus from different dietary sources is variable
• Inorganic phosphorus – 70 -‐100% available
• Organic phosphorus (phytates) ~ 30% available • High Ca:P ra:o can lower phosphorus absorp:on especially if diets are marginal in phosphorus
• Only 18-‐40% of total phosphorus consumed is actually digested
Effects of P Deficiency • Reduced live weight due to loss of appetite
• Leg disorders
• Stiff joints and muscular weakness
• Increased downgrades due to bone breakage
• Reduced hatchability
• Reduced fertility
• Dysfunction of the ovaries
• Inhibition, depression and irregularity of Estrus (livestock)
• Increased vulnerability to disease
P and the Environment • Only ~ 30% dietary phosphorus is retained by poultry
• 70% excretion to environment
• If the phosphorus load on the environment exceeds plant uptake then the
excess will be flushed in to lakes and rivers leading to overgrowth of algae
• Phosphorus is the major pollutant in animal farming areas
• Strategies that reduce environmental impact of phosphorus from animal production
• Use of highly digestible sources of dietary phosphorus e.g. MCP/ DCP
• Phase feeding to more closely match dietary phosphorus to animal needs
• Use Phytase to improve efficiency of phytate phosphorus utilisation from
feed
Phytate
Phytate is the main storage form of phosphorus in plant seeds
• Phytates are phytic acid-mineral complexes (salts of phytic acid)
• Phytate has an important role in physiological functions during dormancy and
germination of seeds
• Phytate is not uniformly distributed throughout the seed, being most prevalent in:
Aleurone layer (bran) of wheat and barley
Germ of corn
Throughout soybean
About Natuzyme
• Premium mul:-‐ac:vity feed enzyme formulated for broiler, layer, duck,
milking cows and aqua feeds.
• Natuzyme contains cellulase, xylanase, beta-‐glucanase, alpha-‐amylase,
protease, pec:nase and phytase and other enzymes.
• Natuzyme enzymes have a wide pH range, stability and temperature
tolerance and high levels of ac:vity.
• Natuzyme has been successfully applied to animal feeds around the
world with excellent results under wide ranging condi:ons.
Enzyme Func-ons
Broiler – Trial Data A • Trial Protocol: To evaluate the effec:veness of different enzyme products
– 1. Natuzyme
– 2. Enzyme R + Phytase
– 3. Enzyme A + Phytase)
– Nega:ve Control Diet vs. Posi:ve Control Diet over a 42 day period.
• Posi:ve Control (PC) Diet: a typical corn / soybean meal used in Mid-‐West USA.
• Nega:ve Control (NC) Diet: PC less 140 Kcal/kg ME and less than 0.1% Available
Phosphorous
• Trial Loca:on: USA, 2010
• Results: Natuzyme provided superior outcome without needing phytase.
Broiler – Trial Data A
Trial Loca:on: USA, 2010
Layer – Trial Data A
• Trial Protocol: Natuzyme was added “on-‐the-‐top” and compared to diet
without enzymes in layers over a 30d period.
• Diet: a basal diet with combina:on of wheat, barley, oat, soybean and
sunflower.
• Trial loca:on: Ukraine 2010
• Results: Natuzyme group increased egg produc:on by 3.6 % and reduced
the number of dirty eggs from 6.1% to 2.5%.
Trial Loca:on: Ukraine 2010
Layer – Trial Data A
Layer – Trial Data B
• Trial Protocol: 350g/Ton of Natuzyme v. 100g/Ton phytase.
• Diet: a basal diet with combina:on of wheat, barley, oat,
soybean and sunflower.
• Trial loca:on: Russia 2011
• Results: Natuzyme group was beper in all categories (profit
margin, produc:vity, dirty eggs, feed costs etc), resul:ng
improved profitability 6.07%.
Layer Trial – B Data
Measure UOM Control + 500 FTU Phytase
Control + 350g/T Natuzyme
Improvement with Natuzyme
Mortality % 2.25% 2.24% -‐0.44%
Av Wt of Egg gram 63.47 63.48 0.02%
Produc:vity % 92.46% 92.68% 0.24%
Feed Cost $US/day/bird 0.0462 0.0455 -‐1.48%
Value of Eggs $US/day/bird 0.0647 0.0649 0.24%
Profit $US/day/bird 0.0186 0.0194 4.50%
Profit Margin % 40.2% 42.7% 6.07%
Trial Loca:on: Russia 2011
Compara-ve Broiler Trial A • Trial Protocol: Compare FCR of leading NSP enzyme products in
commercial feeds
• Diets: Commercial Wheat and Sorghum diets.
• Loca:on: New Zealand 2012
• Results:
1. Wheat diets, Natuzyme was FCR was 0.9% beper than typical compe:tor
2. Sorghum diets, Natuzyme was FCR was 2.44% beper than typical
Compara-ve Broiler Trial A
New Zealand 2012
Compara-ve Broiler Trial B
Trial Loca:on: Massey University, New Zealand 2010
Diet Group AME, kJ/kg DM Improvement AME, kJ/kg DM
Improvement, %
Wheat
Control 3,035 -‐ -‐
Average 3,119 84 2.8%
Natuzyme 3,140 105 3.5%
Sorghum
Control 3,525 -‐ -‐
Average 3,556 31 0.9%
Natuzyme 3,593 68 1.9%
Swine -‐ Trial Data B Diet: Corn / Soybean Meal Species: Weanling pigs Loca:on: Kansas State University, 2007 Protocol: To evaluate the effec:veness of Natuzyme in Nega:ve
Control (NC) diets compared to Posi:ve Control (PC) in a 35d trial on weanling pig performance.
PC Diet: A typical corn / soybean meal used in Mid-‐West USA, NC Diet: PC but with 12.5% Soy Hulls and no an:bio:cs (165kcal/kg
less ME and 1.1% less Protein).
Results: Natuzyme enabled the use of cheaper and lower quality feeds (less energy and protein and no an:bio:cs) on a dose rate of 500g/ton.
Swine – Trial Data B
Trial Loca:on: Kansas State University, 2007
Milking Cows
• Enzyme use in monogastrics is very established science.
• Ruminants are the next stage.
• Pressure to reduce the cost of producing milk and meat
• NDF (Neutral Diges:ble Fibre) = 30—70% of feed. And only ~50% are
digested by animal.
• So poten:al is huge!
• Trial Protocol: Measure the increase in milk produc:on with Natuzyme use
in milking cows.
• Diet: Normal local grass fed diet in Egypt. Natuzyme added at 20g/head/
day
• Trial Loca:on: Egypt, 2009
• Results: Increases Milk Produc:on (2.43% and 1.67%) and Extend
Peak Milk Produc:on Period
Milking Cow – Trial Data
Milking Cow – Trial Data
Trial Loca:on: Egypt 2009, Natuzyme added at 20g/head/day
Milking Cow – Trial Data
• The data in table shows how Natuzyme increases daily milk produc:on and extends Peak Milk Produc:on period.
Source: Egypt 2009, Natuzyme added at 20g/head/day
Period Trial A Trial B
1 to 20d +0.09 % or +0.03 L/d +0.16 % or + 0.05 L/d
21 to 41d +4.57 % or +1.29 L/d +3.04 % or + 0.83 L/d
Average (1-‐41d) +2.43 % or +0.67 L/d +1.67 % or +0.45 L/d
Total (1-‐41d) + 27.7 L +18.4 L
Why Natuzyme?
• Simple -‐ Single product, no need to store or use different
enzymes
• Cost Effec-ve – No need to add phytase or other enzymes
• Flexible – Can be used in all feed scenarios, even if not all
feed quali:es are not known.
• Synergy – e.g. Phytase will work more effec:vely in
combina:on with other enzymes “Phytate availability”
Natuzyme Product Informa-on
• Micro-‐granulated enzymes
• Resistance to heat pelle:ng up to 90 deg C
• Storage for 24mths at room temperature
• 20kg Bags (3 paper and 1 polyethylene layers)
• Typical dosage:
– Broilers: 350 g/Ton of Feed
– Layers: 250g/Ton of Feed
– Milking Cows: 20g/Head/Day