Neon™transfection system

cell protocols

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PBMCThis protocol requires Bu�er T for Primary Cells.

Cell informationCell type/Description PBMC (peripheral blood mononuclear cell)/Primary cell

Characteristics/Species Suspension/Human

Tissue origin Blood

Media RPMI 1640 with L-glutamine and 10% FBS

Morphology Round single cell

Doubling time NA

Subculturing Medium renewal every 2 to 3 days

Culture conditions Temperature: 37°C; atmosphere: air 95%/carbon dioxide (CO2) 5%

Electroporation parametersPulse

voltage (v)Pulse

width (ms)Pulse

numberCell density

(cells/mL)Transfection

ViabilityTip

type

2,150 20 1 2 x 107 23% 95% 10 µL

Peripheral blood mononuclear cells (PBMCs) were transfected using the Neon™ transfection system and 1.0 µg of a plasmid encoding EGFP. 24 hours posttransfection, the cells were analyzed by light (A) and fluorescence microscopy (B).

A B