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CD40-PC5.5 PNB20025Antibody
Specificity: HumanCD40Clone: MAB89Isotype: IgG1mouse
uKatira, A., Holder, M., Pound, J., Gordon, J. “CD40 workshoppanelreport”,1995,inLeucocyteTypingV,Schlossman,S.F.,etalEds,OxfordUniversityPress,547-550.
uChalla, A., Holder, M., Baker, M., Pound, J., Gordon, J., «CD40Workshop Panel report», 1997, Leucocyte Typing VI, WhiteCellDifferentiationAntigens.Kishimoto,T.,etal.,Eds.,GarlandPublishing,Inc.,159-161.
uRousset, F., Garcia, E., Banchereau, J., «Cytokine-inducedproliferation and immunoglobulin production of human Blymphocytes triggered through their CD40 Antigen», 1991, J.Exp.Med.,173,705-710.
uRousset,F.,Garcia,E.,Defrance,T.,Péronne,C.,Vezzio,N.,Hsu,D.H.,Kastelein,R.,Moore,K.W.,BanchereauJ.,«Interleukin10isapotentgrowthanddifferentiationfactorforactivatedhumanBlymphocytes»,1992,Proc.Natl.Acad.Sci.USA.,89,1890-1893.
uLane, P., Traunecker, A., Hubele, S., Inui, S., Lanzavecchia, A.,Gray, D., «Activated human T cells express a ligand for thehuman B cell-associated antigen CD40 which participates inT cell-dependent activation of B lymphocytes», 1992, Eur. J.Immunol.,22,2573-2578.
uBrugnoni,D.,Airo,P.,Graf,D.,Marconi,M.,Molinari,C.,Braga,D.,Malacarne,F.,Soresina,A.,Ugazio,A.G.,Cattaneo,R.,Kroczek,R.A., Notarangelo, L.D., «Ontogeny of CD40 expression byactivated peripheral blood lymphocytes in humans», 1996,Immunol.Letters,49,27-30.
uHenn, V., Slupsky, J.R., Gräfes, M., Anagognostopoulos, I.,Förster, R., Müller-Bergahaus, G., Kroczek, R.A., «CD40 ligandon activated platelets triggers an inflammatory reaction ofendothelialcells»,1998,Nature,391,591-594.
uVallé, A., Zuber, C.E., Defrance, T., Djossou, O., De Rie, M.,Banchereau, J., “Activation on human B lymphocytes throughCD40andinterleukin4”,1989,Eur.J.Immunol.,19,1463-1467.
Antigen Characteristics
References
OtherName: Bp50,TNFreceptor5GeneLocation: 20q12-q13.2GeneName: TNFRSF5MolecularWeight: 44-48kDa
Antibody Characteristics
TheMAB89monoclonalantibodyhasbeenassignedtotheCD40clusterofdifferentiationatthe6thInternationalWorkshoponHumanLeucocyteDifferentiationAntigensinKobe,Japan,in1996(WSCode:CD40.1)
Reagent
Line: IOTestFormat: LiquidSize: 0.5mLStatus: ASR
Example of Results
DS-17464A B2012-13594
Outside the US version
BeckmanCoulter,thestylizedlogoandIOTestaretrademarksofBeckmanCoulter,Inc.andareregisteredintheUSPTO.
ASR:AnalyteSpecificReagent.Analyticalandperformancecharacteristicsarenotestablished.
Normalwholebloodsample
Size Volume Line Status Part#Purified 0.2 mg - - ASR IM1374PE 100 tests 2 mL IOTest ASR IM1936UPC5.5 50 tests 0.5 mL IOTest ASR B20025
Formats available
The CD40 antigen is a type I integral membrane protein of the tumor necrosis factorreceptor(TNFR)superfamily.ThisantigenisfoundonBcelllines,itisstronglyexpressedby interdigitatingcells (IDC),basalepithelialcells,and isalsopresentonmacrophages,someendothelialcells,andfolliculardendriticcells.Itisapan-Bmarker,absentonlyfromterminallydifferentiatedplasmacells.CD40 is implicated in the process of B cell selection in the germinal centre. Studiesdemonstrated that CD40 monoclonal antibodies induce strong homotypic adhesions inrestingBcellsand,togetherwithinterleukin-4(IL-4)maintainthecellcycleofblastsoftheBlineage.TheyalsocanpromotetheswitchtoIgEsecretion.ActivatedBcellsviaCD40antigeninthepresenceofIL-10differentiateintoplasmacellsandsecretelargeamountsof immunoglobulins.CD40bindstoCD154(CD40ligand)onTcells, resulting inBcellgrowthanddifferentiation.
